Polygonatum sibiricum and poria cocos tea and preparation technology thereof
Through ethanol gas impact and activated carbon filtration technology, the problem of the impact of impurities in Polygonatum Poria tea is solved, the purity and usage experience of the tea are improved, and the effect of efficiently removing impurities is achieved.
Patent Information
- Application Number
- CN202411600256.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-11
- Publication Date
- 2025-01-17
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
In the preparation process of existing Polygonatum Poria tea, there are impurities that affect urine excretion, lead to urination difficulties, chest tightness, breathing difficulties, fatigue and lethargy, which affects normal work and study, and it is difficult to achieve high-purity instant tea preparation.
Using ethanol gas impact and activated carbon filtration, the Poria cocos powder and Polygonatum powder are mixed, and then extracted with deionized water and mixed with chloroform-n-butanol solution, sonicated, then mixed with activated carbon, filtered and dried, and finally ethanol is used. Gas wash to produce high-purity Polygonatum Poria cocos tea.
Effectively remove impurities, improve the purity of Polygonatum Poria tea, avoid negative health effects caused by impurities in the original tea beverage, facilitate portability and brewing, and enhance the product usage experience.
Smart Images
Figure BDA0005128099980000081 
Figure BDA0005128099980000091
Abstract
Description
Technical Field
[0001] The invention relates to the field of tea beverages, in particular to polygonatum tuckahoe tea and a preparation process thereof. Background Art
[0002] Polygonatum sibiricum is a yin-tonifying medicine. It is the dried rhizome of Yunnan Polygonatum sibiricum, Polygonatum sibiricum or Polygonatum sibiricum of the Liliaceae family. It has the effects of tonifying qi and nourishing yin, strengthening the spleen, moistening the lungs and benefiting the kidneys; Poria cocos is a diuretic and dampness-inducing medicine. It is the dried sclerotium of the Polyporaceae fungus Poria cocos. It has the functions of promoting diuresis and dampness-inducing, strengthening the spleen, and tranquilizing the mind. Both Polygonatum sibiricum and Poria cocos are rich in polysaccharides, among which Polygonatum sibiricum polysaccharide has the effect of scavenging free radicals, can help reduce cell damage, delay aging, and can also promote insulin secretion, lower blood sugar levels, prevent or assist in the treatment of diabetes. Poria cocos polysaccharide has the effects of reducing swelling and diuresis, nourishing the heart and tranquilizing the mind, strengthening the spleen, strengthening the stomach and improving immunity.
[0003] Soaking Polygonatum and Poria directly in water may cause some negative effects. Polygonatum and Poria not only contain Polygonatum polysaccharides and Poria polysaccharides that are beneficial to the body, but also contain some impurities that may affect the excretion of urine and lead to difficulty in urination; it may cause chest tightness and make it difficult to breathe; it may also make people feel tired and sleepy, affecting normal work and study. In order to facilitate carrying and brewing, this article introduces an instant Polygonatum and Poria tea with higher purity Polygonatum polysaccharides and Poria polysaccharides. Summary of the invention
[0004] The purpose of the present invention is to provide a polygonatum tiliaceus and tuckahoe tea and a preparation process thereof, so as to solve the problems existing in the prior art.
[0005] A polygonatum tiliaceum and tiliaceum tea, which is prepared by shocking a crude polygonatum tiliaceum and tiliaceum extract with ethanol gas, washing, and drying;
[0006] The crude extract of Polygonatum sibiricum and Poria cocos is prepared by mixing Poria cocos powder and Polygonatum sibiricum powder, crudely extracting with deionized water, mixing with chloroform-n-butanol solution, ultrasonicating, extracting the aqueous phase, mixing with activated carbon, filtering, and drying.
[0007] The polygonatum sibiricum powder is obtained by grinding polygonatum sibiricum;
[0008] The Poria cocos powder is obtained by grinding the mycelium to obtain Poria cocos fermentation liquid through fermentation and cultivation.
[0009] A preparation process of polygonatum tiliaceus and tiliaceus tea, the preparation process of polygonatum tiliaceus and tiliaceus tea mainly comprises the following preparation steps:
[0010] (1) inoculating the original Poria cocos seed into a solid culture medium, culturing at 25-27° C. for 6-8 days, inoculating in an ultra-clean workbench with an inoculation amount of 9-11%, placing in a shaker, and culturing in a shake flask for 6-8 days to obtain a primary seed bottle, inoculating the primary seed bottle into a secondary seed bottle in an ultra-clean workbench with an inoculation amount of 9-11%, placing in a shaker, and culturing in a shake flask for 3-5 days, inoculating the cultured bacteria in the secondary seed triangular flask into the fermentation medium on the ultra-clean workbench with an inoculation amount of 5-7%, placing in a shaker, and culturing in a shake flask for 5-6 days to obtain a Poria cocos fermentation liquid;
[0011] (2) filtering the Poria cocos fermentation liquid, taking the mycelium, centrifuging, washing with deionized water for 5 to 7 times, drying at -10 to 0°C for 22 to 26 hours, grinding to obtain Poria cocos powder; grinding Polygonatum sibiricum to obtain Polygonatum sibiricum powder; mixing Poria cocos powder and Polygonatum sibiricum powder in a mass ratio of 1:1, adding deionized water 10 to 20 times the mass of Poria cocos powder, soaking at 75 to 85°C and 200 to 300 r / min for 4 to 6 hours, filtering and taking the filtrate to obtain Polygonatum sibiricum Poria primary extract, adding Polygonatum sibiricum Poria primary extract by volume 0.2-0.3 times of chloroform-n-butanol solution, ultrasonicate for 30-40 minutes, centrifuge, take the aqueous phase, repeat the operation 8-10 times, add activated carbon with the mass of 0.1-0.2 times of the initial extract of Polygonatum sibiricum and Poria cocos, ultrasonicate for 2-4 hours, filter and take the filtrate, dry and grind to obtain the crude extract of Polygonatum sibiricum and Poria cocos; spread the crude extract of Polygonatum sibiricum and Poria cocos in a reaction container, impact the crude extract of Polygonatum sibiricum and Poria cocos with ethanol gas at 80-84°C for 22-26 hours, wash with ethanol 3-5 times, dry to obtain Polygonatum sibiricum and Poria cocos tea.
[0012] As an optimization, the culture conditions of the shake flask culture in step (1) are 100-200 rpm and 25-27°C.
[0013] As an optimization, the grinding condition in step (2) is grinding to 20-40 mesh.
[0014] As an optimization, the chloroform-n-butanol solution in step (2) is prepared by uniformly mixing chloroform and n-butanol in a volume ratio of 4:1.
[0015] As an optimization, the activated carbon in step (2) is 200-300 mesh.
[0016] As an optimization, the specific operation of the drying in step (2) is vacuum drying at -10 to 0°C for 48 to 50 hours.
[0017] Compared with the prior art, the beneficial effects achieved by the present invention are:
[0018] When preparing the polygonatum sibiricum and tuckahoe tea, the present invention comprises the following steps: fermenting and culturing tuckahoe to obtain tuckahoe fermentation liquid, grinding mycelium to obtain tuckahoe powder; grinding polygonatum sibiricum to obtain polygonatum sibiricum powder; mixing tuckahoe powder and polygonatum sibiricum powder, crudely extracting with deionized water, mixing with chloroform-n-butanol solution, ultrasonicating, extracting water phase, mixing with activated carbon, filtering, and drying to obtain a polygonatum sibiricum and tuckahoe crude extract; and shocking the polygonatum sibiricum and tuckahoe crude extract with ethanol gas, washing, and drying to obtain the polygonatum sibiricum and tuckahoe tea.
[0019] First, Poria cocos is fermented and cultured to obtain Poria cocos fermentation liquid, and the mycelium is ground to obtain Poria cocos powder; Polygonatum sibiricum is ground to obtain Polygonatum sibiricum powder; Poria cocos powder and Polygonatum sibiricum powder are mixed, and crude extraction is performed with deionized water, and then mixed with chloroform-n-butanol solution, ultrasonicated, and the aqueous phase is taken, and the Poria cocos powder and Polygonatum sibiricum powder are mixed, and crude extraction is performed with deionized water, so that the water-soluble polysaccharides in Polygonatum sibiricum and Poria cocos can be well extracted, which is convenient for brewing, and then mixed with chloroform-n-butanol solution, ultrasonicated, and the protein in the impurities in the extract can be dissolved in the chloroform-n-butanol solution, thereby removing the protein impurities therein.
[0020] Secondly, the extract is mixed with activated carbon, filtered and dried to obtain a crude extract of polygonatum and tuckahoe; the crude extract of polygonatum and tuckahoe is impacted with ethanol gas, washed and dried to obtain polygonatum and tuckahoe tea. The activated carbon can not only decolorize, but also adsorb impurities in the extract. The crude extract of polygonatum and tuckahoe is impacted with ethanol gas. Since both tuckahoe polysaccharide and polygonatum polysaccharide are insoluble in ethanol, and the extract is ground to increase the contact area with ethanol gas, some of the impurities soluble in ethanol will be separated along with the ethanol gas, thereby further achieving the purification effect. DETAILED DESCRIPTION
[0021] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.
[0022] Embodiment 1:
[0023] A preparation process of polygonatum tiliaceus and tuckahoe tea mainly comprises the following preparation steps:
[0024] (1) The original Poria cocos seed was inoculated into a solid culture medium, cultured at 25° C. for 6 days, inoculated in an ultra-clean workbench with an inoculation amount of 9%, placed in a shaker, and cultured in a shake flask for 6 days to obtain a primary seed bottle, inoculated the primary seed bottle into a secondary seed bottle in an ultra-clean workbench with an inoculation amount of 9%, placed in a shaker, and cultured in a shake flask for 3 days, and inoculated the cultured bacteria in the secondary seed triangular flask into the fermentation medium of the ultra-clean workbench with an inoculation amount of 5%, placed in a shaker, and cultured in a shake flask for 5 days to obtain a Poria cocos fermentation liquid;
[0025] (2) Filter the Poria fermentation liquid, take the mycelium, centrifuge, wash with deionized water for 5 times, dry at -10°C for 22 hours, grind to obtain Poria powder; grind Polygonatum to obtain Polygonatum powder; mix Poria powder and Polygonatum powder in a mass ratio of 1:1, add deionized water 10 times the mass of Poria powder, soak at 75°C, 200r / min for 4 hours, filter and take the filtrate to obtain Polygonatum and Poria primary extract, add chloroform-n-butanol solution 0.2 times the volume of Polygonatum and Poria primary extract, ultrasonicate for 30 minutes, centrifuge, take the aqueous phase, repeat the operation 8 times, add activated carbon 0.1 times the mass of Polygonatum and Poria primary extract, ultrasonicate for 2 hours, filter and take the filtrate, dry, grind to obtain Polygonatum and Poria crude extract; spread the Polygonatum and Poria crude extract in a reaction container, shock the Polygonatum and Poria crude extract with 80°C ethanol gas for 22 hours, wash with ethanol 3 times, dry, and obtain Polygonatum and Poria tea.
[0026] As an optimization, the culture conditions of the shake flask culture in step (1) are 100 rpm and 25°C.
[0027] As an optimization, the grinding condition in step (2) is grinding to 20 mesh.
[0028] As an optimization, the chloroform-n-butanol solution in step (2) is prepared by uniformly mixing chloroform and n-butanol in a volume ratio of 4:1.
[0029] As an optimization, the activated carbon in step (2) is 200 mesh.
[0030] As an optimization, the specific operation of the drying in step (2) is vacuum drying at -10°C for 48 hours.
[0031] Embodiment 2:
[0032] A preparation process of polygonatum tiliaceus and tuckahoe tea mainly comprises the following preparation steps:
[0033] (1) The original Poria cocos seed was inoculated into a solid culture medium, cultured at 26° C. for 7 days, inoculated in a clean bench with an inoculation amount of 10%, placed in a shaker, and cultured in a shaker for 7 days to obtain a primary seed bottle, inoculated the primary seed bottle into a secondary seed bottle in a clean bench with an inoculation amount of 10%, placed in a shaker, and cultured in a shaker for 4 days, and inoculated the cultured bacteria in the secondary seed triangular flask into the fermentation medium of the clean bench with an inoculation amount of 6%, placed in a shaker, and cultured in a shaker for 5.5 days to obtain a Poria cocos fermentation liquid;
[0034] (2) Filter the Poria fermentation liquid, take the mycelium, centrifuge, wash with deionized water for 6 times, dry at -5°C for 24 hours, grind to obtain Poria powder; grind Polygonatum to obtain Polygonatum powder; mix Poria powder and Polygonatum powder in a mass ratio of 1:1, add deionized water 15 times the mass of Poria powder, soak at 80°C, 250r / min for 5 hours, filter and take the filtrate to obtain Polygonatum and Poria primary extract, add chloroform-n-butanol solution 0.25 times the volume of Polygonatum and Poria primary extract, ultrasonicate for 35 minutes, centrifuge, take the aqueous phase, repeat the operation 9 times, add activated carbon 0.15 times the mass of Polygonatum and Poria primary extract, ultrasonicate for 3 hours, filter and take the filtrate, dry, grind to obtain Polygonatum and Poria crude extract; spread the Polygonatum and Poria crude extract in a reaction container, shock the Polygonatum and Poria crude extract with 82°C ethanol gas for 24 hours, wash with ethanol 4 times, dry, grind, and obtain Polygonatum and Poria tea.
[0035] As an optimization, the culture conditions of the shake flask culture in step (1) are 150 rpm and 26°C.
[0036] As an optimization, the grinding condition in step (2) is grinding to 30 mesh.
[0037] As an optimization, the chloroform-n-butanol solution in step (2) is prepared by uniformly mixing chloroform and n-butanol in a volume ratio of 4:1.
[0038] As an optimization, the activated carbon in step (2) is 250 mesh.
[0039] As an optimization, the specific operation of the drying in step (2) is vacuum drying at -5°C for 49 hours.
[0040] Embodiment 3:
[0041] A preparation process of polygonatum tiliaceus and tuckahoe tea mainly comprises the following preparation steps:
[0042] (1) The original Poria cocos seed was inoculated into a solid culture medium, cultured at 27° C. for 8 days, inoculated in an ultra-clean workbench with an inoculation amount of 11%, placed in a shaker, and cultured in a shake flask for 8 days to obtain a primary seed bottle, inoculated the primary seed bottle into a secondary seed bottle in an ultra-clean workbench with an inoculation amount of 11%, placed in a shaker, and cultured in a shake flask for 5 days, and inoculated the cultured bacteria in the secondary seed triangular flask into the fermentation medium of the ultra-clean workbench with an inoculation amount of 7%, placed in a shaker, and cultured in a shake flask for 6 days to obtain a Poria cocos fermentation liquid;
[0043] (2) Filter the Poria fermentation liquid, take the mycelium, centrifuge, wash with deionized water for 7 times, dry at 0°C for 26 hours, grind to obtain Poria powder; grind Polygonatum to obtain Polygonatum powder; mix Poria powder and Polygonatum powder in a mass ratio of 1:1, add deionized water 20 times the mass of Poria powder, soak at 85°C, 300r / min for 6 hours, filter and take the filtrate to obtain Polygonatum and Poria primary extract, add chloroform-n-butanol solution 0.3 times the volume of Polygonatum and Poria primary extract, ultrasonicate for 40 minutes, centrifuge, take the aqueous phase, repeat the operation 10 times, add activated carbon 0.2 times the mass of Polygonatum and Poria primary extract, ultrasonicate for 4 hours, filter and take the filtrate, dry, grind to obtain Polygonatum and Poria crude extract; spread the Polygonatum and Poria crude extract in a reaction container, shock the Polygonatum and Poria crude extract with 84°C ethanol gas for 26 hours, wash with ethanol 5 times, dry to obtain Polygonatum and Poria tea.
[0044] As an optimization, the culture conditions of the shake flask culture in step (1) are 200 rpm and 27°C.
[0045] As an optimization, the grinding condition in step (2) is grinding to 40 mesh.
[0046] As an optimization, the chloroform-n-butanol solution in step (2) is prepared by uniformly mixing chloroform and n-butanol in a volume ratio of 4:1.
[0047] As an optimization, the activated carbon in step (2) is 300 mesh.
[0048] As an optimization, the specific operation of the drying in step (2) is vacuum drying at 0°C for 50 hours.
[0049] Comparative Example 1:
[0050] A preparation process of polygonatum tiliaceus and tuckahoe tea mainly comprises the following preparation steps:
[0051] (1) The original Poria cocos seed was inoculated into a solid culture medium, cultured at 26° C. for 7 days, inoculated in a clean bench with an inoculation amount of 10%, placed in a shaker, and cultured in a shaker for 7 days to obtain a primary seed bottle, inoculated the primary seed bottle into a secondary seed bottle in a clean bench with an inoculation amount of 10%, placed in a shaker, and cultured in a shaker for 4 days, and inoculated the cultured bacteria in the secondary seed triangular flask into the fermentation medium of the clean bench with an inoculation amount of 6%, placed in a shaker, and cultured in a shaker for 5.5 days to obtain a Poria cocos fermentation liquid;
[0052] (2) Filter the Poria fermentation liquid, take out the mycelium, centrifuge, wash with deionized water for 6 times, dry at -5°C for 24 hours, grind to obtain Poria powder; grind Polygonatum sibiricum to obtain Polygonatum sibiricum powder; mix Poria powder and Polygonatum sibiricum powder in a mass ratio of 1:1, add deionized water 15 times the mass of Poria powder, soak at 80°C, 250r / min for 5 hours, filter and take the filtrate to obtain Polygonatum sibiricum and Poria primary extract, add activated carbon 0.15 times the mass of Polygonatum sibiricum and Poria primary extract, ultrasonicate for 3 hours, filter and take the filtrate, dry, grind to obtain Polygonatum sibiricum and Poria crude extract; spread the Polygonatum sibiricum and Poria crude extract in a reaction container, impact the Polygonatum sibiricum and Poria crude extract with 82°C ethanol gas for 24 hours, wash with ethanol 4 times, dry, grind, and obtain Polygonatum sibiricum and Poria tea.
[0053] As an optimization, the culture conditions of the shake flask culture in step (1) are 150 rpm and 26°C.
[0054] As an optimization, the grinding condition in step (2) is grinding to 30 mesh.
[0055] As an optimization, the activated carbon in step (2) is 250 mesh.
[0056] As an optimization, the specific operation of the drying in step (2) is vacuum drying at -5°C for 49 hours.
[0057] Comparative Example 2
[0058] A preparation process of polygonatum tiliaceus and tuckahoe tea mainly comprises the following preparation steps:
[0059] (1) The original Poria cocos seed was inoculated into a solid culture medium, cultured at 26° C. for 7 days, inoculated in a clean bench with an inoculation amount of 10%, placed in a shaker, and cultured in a shaker for 7 days to obtain a primary seed bottle, inoculated the primary seed bottle into a secondary seed bottle in a clean bench with an inoculation amount of 10%, placed in a shaker, and cultured in a shaker for 4 days, and inoculated the cultured bacteria in the secondary seed triangular flask into the fermentation medium of the clean bench with an inoculation amount of 6%, placed in a shaker, and cultured in a shaker for 5.5 days to obtain a Poria cocos fermentation liquid;
[0060] (2) Filter the Poria fermentation liquid, take the mycelium, centrifuge, wash with deionized water for 6 times, dry at -5°C for 24 hours, grind to obtain Poria powder; grind Polygonatum to obtain Polygonatum powder; Poria powder and Polygonatum powder are mixed in a mass ratio of 1:1, add deionized water 15 times the mass of Poria powder, soak at 80°C, 250r / min for 5 hours, filter and take the filtrate to obtain Polygonatum and Poria primary extract, add chloroform-n-butanol solution 0.25 times the volume of Polygonatum and Poria primary extract, ultrasonicate for 35 minutes, centrifuge, take the aqueous phase, repeat the operation 9 times, dry, grind, and obtain Polygonatum and Poria crude extract; spread the Polygonatum and Poria crude extract in a reaction container, impact the Polygonatum and Poria crude extract with 82°C ethanol gas for 24 hours, wash with ethanol 4 times, dry, grind, and obtain Polygonatum and Poria tea.
[0061] As an optimization, the culture conditions of the shake flask culture in step (1) are 150 rpm and 26°C.
[0062] As an optimization, the grinding condition in step (2) is grinding to 30 mesh.
[0063] As an optimization, the chloroform-n-butanol solution in step (2) is prepared by uniformly mixing chloroform and n-butanol in a volume ratio of 4:1.
[0064] As an optimization, the specific operation of the drying in step (2) is vacuum drying at -5°C for 49 hours.
[0065] Comparative Example 3
[0066] A preparation process of polygonatum tiliaceus and tuckahoe tea mainly comprises the following preparation steps:
[0067] (1) The original Poria cocos seed was inoculated into a solid culture medium, cultured at 26° C. for 7 days, inoculated in a clean bench with an inoculation amount of 10%, placed in a shaker, and cultured in a shaker for 7 days to obtain a primary seed bottle, inoculated the primary seed bottle into a secondary seed bottle in a clean bench with an inoculation amount of 10%, placed in a shaker, and cultured in a shaker for 4 days, and inoculated the cultured bacteria in the secondary seed triangular flask into the fermentation medium of the clean bench with an inoculation amount of 6%, placed in a shaker, and cultured in a shaker for 5.5 days to obtain a Poria cocos fermentation liquid;
[0068] (2) Filter the Poria fermentation liquid, take the mycelium, centrifuge, wash with deionized water for 6 times, dry at -5°C for 24 hours, grind to obtain Poria powder; grind Polygonatum sibiricum to obtain Polygonatum sibiricum powder; mix Poria powder and Polygonatum sibiricum powder in a mass ratio of 1:1, add deionized water 15 times the mass of Poria powder, soak at 80°C, 250r / min for 5 hours, filter and take the filtrate to obtain Polygonatum sibiricum Poria primary extract, add chloroform-n-butanol solution 0.25 times the volume of Polygonatum sibiricum Poria primary extract, ultrasonicate for 35 minutes, centrifuge, take the aqueous phase, repeat the operation 9 times, add activated carbon 0.15 times the mass of Polygonatum sibiricum Poria primary extract, ultrasonicate for 3 hours, filter and take the filtrate, dry, grind, and obtain Polygonatum sibiricum Poria tea.
[0069] As an optimization, the culture conditions of the shake flask culture in step (1) are 150 rpm and 26°C.
[0070] As an optimization, the grinding condition in step (2) is grinding to 30 mesh.
[0071] As an optimization, the chloroform-n-butanol solution in step (2) is prepared by uniformly mixing chloroform and n-butanol in a volume ratio of 4:1.
[0072] As an optimization, the activated carbon in step (2) is 250 mesh.
[0073] As an optimization, the specific operation of the drying in step (2) is vacuum drying at -5°C for 49 hours.
[0074] Test Example 1:
[0075] Purity testing
[0076] The Huangjing Fuling tea was mixed with 80℃ deionized water at a mass ratio of 1:10 to prepare a diluent, and the diluent, 5% phenol by mass fraction and 98.3% concentrated sulfuric acid were mixed evenly at a volume ratio of 1:2:6, and allowed to stand for 30 minutes. The absorbance value was measured at a wavelength of 490nm, and the polysaccharide content was calculated, and then the purity was calculated, where the purity was polysaccharide mass / Huangjing Fuling tea mass × 100%. The results are shown in Table 1.
[0077] Table 1
[0078]
[0079]
[0080] From the comparison of the experimental data in Table 1, it can be found that the polygonatum sibiricum and tuckahoe tea prepared by the present invention has a higher purity.
[0081] From the comparison of the experimental data of Examples 1, 2, 3 and Comparative Example 1 in Table 1, it can be found that the purity of Examples 1, 2, and 3 is higher than that of Comparative Example 1, which indicates that after mixing with the chloroform-n-butanol solution and ultrasonication, the protein in the impurities in the extract can be dissolved in the chloroform-n-butanol solution, thereby removing the protein impurities therein, thereby improving the purity of the product;
[0082] From the comparison of the experimental data of Examples 1, 2, 3 and Comparative Example 2, it can be found that the purity of Examples 1, 2, 3 is higher than that of Comparative Example 2, which shows that the activated carbon can not only play a decolorizing role, but also can adsorb impurities in the extract, thereby improving the purity of the product;
[0083] From the comparison of the experimental data of Examples 1, 2, 3 and Comparative Example 3, it can be found that the purity of Examples 1, 2, 3 is higher than that of Comparative Example 3, which shows that the extract is ground to increase the contact area with the ethanol gas, and then the extract is impacted with ethanol gas. Since both Poria polysaccharide and Polygonatum sibiricum polysaccharide are insoluble in ethanol, some of the impurities soluble in ethanol will be separated with the ethanol gas, thereby improving the purity of the product.
[0084] The specific implementation methods described above further illustrate the objectives, technical solutions and beneficial effects of the present invention in detail. It should be understood that the above description is only a specific implementation method of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.
Claims
1. A polygonatum tiliaceus and tuckahoe tea, characterized in that: The polygonatum sibiricum and tuckahoe tea is prepared by shocking the crude polygonatum sibiricum and tuckahoe extract with ethanol gas, washing, and drying; The crude extract of Polygonatum sibiricum and Poria cocos is prepared by mixing Poria cocos powder and Polygonatum sibiricum powder, crudely extracting with deionized water, mixing with chloroform-n-butanol solution, ultrasonicating, extracting the aqueous phase, mixing with activated carbon, filtering, and drying. The polygonatum sibiricum powder is obtained by grinding polygonatum sibiricum; The Poria powder is obtained by grinding the mycelium to obtain Poria fermentation liquid through fermentation and cultivation of Poria.
2. A preparation process of Polygonatum sibiricum and Poria cocos tea, characterized in that: The preparation process of the polygonatum tuckahoe tea mainly includes the following preparation steps: (1) inoculating the original Poria cocos seed into a solid culture medium, culturing at 25-27° C. for 6-8 days, inoculating in an ultra-clean workbench with an inoculation amount of 9-11%, placing in a shaker, and culturing in a shake flask for 6-8 days to obtain a primary seed bottle, inoculating the primary seed bottle into a secondary seed bottle in an ultra-clean workbench with an inoculation amount of 9-11%, placing in a shaker, and culturing in a shake flask for 3-5 days, inoculating the cultured bacteria in the secondary seed triangular flask into the fermentation medium on the ultra-clean workbench with an inoculation amount of 5-7%, placing in a shaker, and culturing in a shake flask for 5-6 days to obtain a Poria cocos fermentation liquid; (2) filtering the Poria cocos fermentation liquid, taking the mycelium, centrifuging, washing with deionized water for 5 to 7 times, drying at -10 to 0°C for 22 to 26 hours, grinding to obtain Poria cocos powder; grinding Polygonatum sibiricum to obtain Polygonatum sibiricum powder; mixing Poria cocos powder and Polygonatum sibiricum powder in a mass ratio of 1:1, adding deionized water 10 to 20 times the mass of Poria cocos powder, soaking at 75 to 85°C and 200 to 300 r / min for 4 to 6 hours, filtering and taking the filtrate to obtain Polygonatum sibiricum Poria primary extract, adding Polygonatum sibiricum Poria primary extract by volume 0.2-0.3 times of chloroform-n-butanol solution, ultrasonicate for 30-40 minutes, centrifuge, take the aqueous phase, repeat the operation 8-10 times, add activated carbon with the mass of 0.1-0.2 times of the initial extract of Polygonatum sibiricum and Poria cocos, ultrasonicate for 2-4 hours, filter and take the filtrate, dry and grind to obtain the crude extract of Polygonatum sibiricum and Poria cocos; spread the crude extract of Polygonatum sibiricum and Poria cocos in a reaction container, impact the crude extract of Polygonatum sibiricum and Poria cocos with ethanol gas at 80-84°C for 22-26 hours, wash with ethanol 3-5 times, dry to obtain Polygonatum sibiricum and Poria cocos tea.
3. The preparation process of a polygonatum tuckahoe tea according to claim 2, characterized in that: The culture conditions of the shake flask culture in step (1) are 100-200 rpm and 25-27°C.
4. The preparation process of a polygonatum tuckahoe tea according to claim 2, characterized in that: The grinding condition in step (2) is grinding to 20-40 mesh.
5. The preparation process of a polygonatum tuckahoe tea according to claim 2, characterized in that: The chloroform-n-butanol solution in step (2) is prepared by uniformly mixing chloroform and n-butanol in a volume ratio of 4:
1.
6. The preparation process of a polygonatum tuckahoe tea according to claim 2, characterized in that: The activated carbon in step (2) has a mesh size of 200 to 300.
7. The preparation process of the polygonatum tuckahoe tea according to claim 2, characterized in that: The specific operation of the drying in step (2) is vacuum drying at -10 to 0°C for 48 to 50 hours.