A composition with oil control and anti-inflammatory efficacy, and a preparation method and application thereof

Abstract

The application belongs to the field of cosmetics, and discloses a composition with oil control and anti-inflammatory effects, and a preparation method and application thereof.The composition with oil control and anti-inflammatory effects comprises lotus seed extract, medicinal fomes fomentarius extract and myrtle fruit extract, and the mass ratio of the lotus seed extract, the medicinal fomes fomentarius extract and the myrtle fruit extract is (1-6):(0.05-0.2):(1-6).The components in the composition have a synergistic effect, can not only exhibit excellent instant effects, but also have remarkable long-term oil control effects, and can bring excellent oil control and anti-inflammatory effects to the skin.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetics, in particular to a composition with oil control and anti-inflammatory effects, and a preparation method and application thereof. BACKGROUND

[0002] Many people have encountered the phenomenon of excessive oil on the face or excessive oil in local areas (such as the T zone). Where does the oil on the skin come from? In fact, the oil on the surface of the skin is not really oil, but the product of secretion and excretion of sebaceous glands. Sebaceous glands are a special tissue, mainly derived from keratinocytes and sebaceous glands, which are most active during puberty and can secrete a large amount of oil, which can help lubricate the skin and keep it moist and soft.

[0003] However, there are many factors that affect sebaceous secretion, such as sebaceous gland size, location, race, genetics, age, gender, diet, season, and lifestyle, which can affect skin oil secretion. When sebaceous glands secrete too much, the skin will look oily, the pores will be enlarged, and many bacteria, fungi and parasites will have a good living environment, which can easily cause or aggravate a series of skin problems, such as acne, blackheads, whiteheads, and skin inflammation.

[0004] With the pursuit of beauty by the young generation and the popularity of the Internet, the consumption of cosmetics has been increasing year by year. Because everyone's skin condition is different, the demand for cosmetics is also different, among which, oil control and anti-inflammatory products are needed by more and more people. In addition to the demand for better skin oil control, consumers also hope to solve the problem of skin inflammation caused by excessive oil secretion to a greater extent. However, the oil control and anti-inflammatory products on the market have the defects of not obvious immediate effect, not outstanding long-term effect, and less improvement of skin inflammation. SUMMARY

[0005] The purpose of the present application is to overcome the shortcomings of the background art, and to provide a composition with oil control and anti-inflammatory effects, and a preparation method and application thereof. The components in the composition of the present application work synergistically, not only showing excellent immediate effect, but also having remarkable long-term oil control effect, and bringing excellent oil control and anti-inflammatory effect to the skin.

[0006] To achieve the purpose of the present application, the composition with oil control and anti-inflammatory effects of the present application comprises lotus seed extract, medicinal fomes officinalis extract and myrtle fruit extract.

[0007] Further, in some embodiments of the present application, the composition with oil control and anti-inflammatory effects consists of lotus seed extract, medicinal fomes officinalis extract and myrtle fruit extract.

[0008] Preferably, in some embodiments of the present application, the lotus seed extract, the phellinus baumii extract and the myrtle fruit extract are in a mass ratio of (1-6):(0.05-0.2):(1-6).

[0009] Further preferably, in some embodiments of the present application, the lotus seed extract, the phellinus baumii extract and the myrtle fruit extract are in a mass ratio of (1-4):(0.05-0.1):(1-4).

[0010] Further preferably, in some embodiments of the present application, the lotus seed extract, the phellinus baumii extract and the myrtle fruit extract are in a mass ratio of 4:(0.05-0.1):(3-4).

[0011] More preferably, the lotus seed extract, the phellinus baumii extract and the myrtle fruit extract are in a mass ratio of 4:0.05:3 or 4:0.1:4.

[0012] Further, in some embodiments of the present application, the preparation method of the lotus seed extract comprises the following steps:

[0013] 1) selecting the crushed germinated lotus seeds to ferment at room temperature, and the fermentation time is 3-6 days;

[0014] 2) adding polyhydric alcohol in an amount of 10-20 times of the germinated lotus seeds, and the extraction temperature is 45-65°C, the extraction time is 3-5h, and the extraction times is 1-2 times;

[0015] 3) cooling the extraction liquid to a temperature of 25°C or below, and filtering the extraction liquid with 100-200 mesh gauze to obtain a primary filtrate;

[0016] 4) standing, centrifuging and filtering the primary filtrate to obtain a secondary filtrate;

[0017] 5) concentrating the secondary filtrate under reduced pressure to 1 / 4-1 / 2 of the original weight, adding deionized water in an amount of 1-10 times of the concentrated liquid, mixing, filtering, and collecting the filtrate to obtain the lotus seed extract.

[0018] Further, in some embodiments of the present application, the preparation method of the phellinus baumii extract comprises the following steps:

[0019] 1) selecting the dried phellinus baumii to crush and sieve to obtain phellinus baumii powder;

[0020] 2) adding deionized water in an amount of 1-10 times of the obtained phellinus baumii powder, and extracting 1-3 times at an extraction temperature of 45-60°C to collect the extraction liquid;

[0021] 3) The extract obtained in the previous step is cooled to 20-30℃, and filtered to obtain a primary filtrate;

[0022] 4) The primary filtrate obtained in the previous step is centrifuged, filtered, and the filtrate is collected to obtain the medicinal Fomes fomentarius extract.

[0023] Further, in some embodiments of the present application, the preparation method of the Myrtus communis fruit extract comprises the following steps:

[0024] 1) The fruits of Myrtus communis are selected and soaked in 10-20 times the amount of a polyol for 4-6 hours;

[0025] 2) The fruits are extracted by ultrasonic oscillation, filtered, and the filtrate is collected;

[0026] 3) A clarifying agent is added, and the mixture is left to stand at low temperature for 3-5 hours, and then filtered;

[0027] 4) The filtrate is concentrated under reduced pressure at a temperature of 50-60℃ to obtain a concentrated solution;

[0028] 5) 10-20 times the weight of propylene glycol is added to the concentrated solution, the mixture is mixed and filtered, and the filtrate is collected to obtain the Myrtus communis fruit extract.

[0029] On the other hand, the present application also provides a preparation method of the aforementioned composition with oil control and anti-inflammatory effects, which comprises weighing the lotus seed extract, the medicinal Fomes fomentarius extract, and the Myrtus communis fruit extract according to a certain proportion, and mixing them to obtain the composition.

[0030] Optionally, in the preparation method of the composition with oil control and anti-inflammatory effects, a solvent can be added before the lotus seed extract, the medicinal Fomes fomentarius extract, and the Myrtus communis fruit extract are mixed; preferably, the solvent is one or more of water, butylene glycol, glycerol, propylene glycol, and ethanol.

[0031] In another aspect, the present application also provides a cosmetic product containing the aforementioned composition of the present application.

[0032] Further, in some embodiments of the present application, the cosmetic product includes, but is not limited to, a water agent, an emulsion, a spray, a cream, a mask, and a foundation.

[0033] Compared with the prior art, the present application has the following advantages:

[0034] (1) The oil control and anti-inflammatory composition of the present application can significantly reduce the level of sebum secretion, reduce facial shine, and fundamentally solve the problem of oiliness, not only exhibiting excellent immediate effects, but also having remarkable long-term oil control effects.

[0035] (2) The oil control and anti-inflammatory composition of the present application can reduce the expression of inflammatory factors and inhibit the occurrence of inflammation, and the skin inflammation problem is improved to a greater extent.

[0036] (3) The oil-control, anti-inflammatory composition of the present application is compounded by three raw materials, and the components synergize to bring more excellent oil-control and anti-inflammatory effects to the skin, achieving excellent effects that cannot be achieved by adding a single component. BRIEF DESCRIPTION OF DRAWINGS

[0037] Figure 1 is a TNF-α content (pg / ml) graph of Example 3, Example 7, Example 8, Example 9, Comparative Example 1-3, and the control group in Experimental Example 2 of the present application;

[0038] Figure 2 is an IL-6 content (pg / ml) graph of Example 3, Example 7, Example 8, Example 9, Comparative Example 1-3, and the control group in Experimental Example 2 of the present application;

[0039] Figure 3 is a skin oil content (A.U.) graph of Example 3, Example 7, Example 8, Example 9, and Comparative Example 1-3 in Experimental Example 4 of the present application;

[0040] Figure 4 is a trans-epidermal water loss Tewl value (g / h·m 2 ) graph after using different compositions in Experimental Example 3 of the present application;

[0041] Figure 5 is a skin erythema index a* value (A.U.) graph after using different compositions in Experimental Example 3 of the present application. DETAILED DESCRIPTION

[0042] In order to make the objects, technical solutions, and advantages of the present application clearer, the present application is further described in detail below with reference to the drawings and examples. Additional aspects and advantages of the present application will be described in the following description, will become apparent from the following description, or will be learned from practice of the present application. It should be understood that the following description is merely intended to explain the present application and is not intended to limit the present application.

[0043] When a range, a preferred range, or a range defined by a series of upper preferred values and lower preferred values is used to express an equivalent, a concentration, or other value or parameter, it should be understood that all ranges formed by any pairings of an upper range limit or preferred value with a lower range limit or preferred value, regardless of whether the range is separately disclosed, are specifically disclosed. For example, when a range "1 to 5" is disclosed, the described range should be interpreted to include ranges "1 to 4", "1 to 3", "1 to 2", "1 to 2 and 4 to 5", "1 to 3 and 5", etc. When numerical ranges are described herein, unless otherwise stated, the range is intended to include the end values and all integers and fractions within the range, unless otherwise stated.

[0044] The indefinite articles "a" and "an," as used herein in the specification, unless clearly indicated to the contrary, should be understood to mean "at least one." The use of the term "optionally" with respect to any feature, structure, material, or characteristic means that such feature, structure, material, or characteristic is contemplated to be present or absent, and that the application can therefore comprise a composition, method, or article of manufacture without such feature, structure, material, or characteristic.

[0045] In addition, the terms "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," and so forth, as used herein, are not necessarily to be construed as referring to the same embodiment or example, unless otherwise specifically indicated. Moreover, various embodiments can be described herein using terminology such as "comprising", "including", "having", "characterized by", "including", "carrying", "or", "based on", "controlled by", "controlled to", "operated by", "operated to", "executed by", "executed to", "capable of", "configured to", "arranged to", "denoted by", "driven by", "established by", "executed by", "executed to", "operated by", "operated to", "performed by", "performed to", "provided by", "regulated by", "regulated to", "set by", "set to", "specified by", "utilized by", or the like. Such terminology, and similar terminology, is intended to be understood in the context as describing various embodiments without necessarily being limited to only a single embodiment or example.

[0046] The raw materials and devices used in the present application are all common raw materials and devices in the art, and are all commercially available products, unless otherwise specified. The methods used in the present application are all conventional methods in the art, unless otherwise specified.

[0047] The present application also has other various implementable technical solutions, which are not listed one by one here, and the technical solutions claimed in the claims of the present application are all implementable.

[0048] In the following examples and comparative examples of the present application, the composition with oil control and anti-inflammatory effects is prepared by the following process:

[0049] (1) Preparation of lotus seed extract: The crushed germinated lotus seeds are fermented at room temperature for 3 days. A polyol is added in an amount of 15 times the amount of the germinated lotus seeds, and the extraction is carried out at a temperature of 50°C for 3 hours, with 2 extraction times. The temperature of the extraction liquid is cooled to 25°C or below, and the initial filtrate is obtained by filtering through 100-200 mesh gauze. The initial filtrate is allowed to stand for 22 hours, centrifuged, and filtered to obtain the secondary filtrate. The secondary filtrate is concentrated under reduced pressure to 1 / 3 of the original weight, deionized water is added in an amount of 10 times the weight of the concentrated liquid, mixed, filtered, and the filtrate is collected to obtain the lotus seed extract.

[0050] (2) Preparation of medicinal Fomes fomentarius extract: Deionized water is added in an amount of 10 times the amount of the sieved medicinal Fomes fomentarius powder, and the extraction is carried out 3 times at a temperature of 55°C, and the extraction liquid is collected. The obtained extraction liquid is cooled to 25°C, and the initial filtrate is obtained by filtering. The obtained initial filtrate is centrifuged, filtered, and the filtrate is collected to obtain the medicinal Fomes fomentarius extract.

[0051] (3) Preparation of Myrtus communis fruit extract: take the fruit of Myrtus communis and soak in 20 times of polyhydric alcohol for 5 hours; extract by ultrasonic oscillation, filter and collect the filtrate; then add clarifying agent and stand for 4 hours at low temperature, filter; place the filtrate in a rotary evaporator and concentrate under reduced pressure, with the temperature being 60°C, to obtain concentrated solution; add 20 times of propylene glycol by weight of the concentrated solution, mix well, filter and collect the filtrate to obtain Myrtus communis fruit extract.

[0052] (4) Mix the solutions obtained in steps (1), (2) and (3) according to the desired ratio to obtain the composition for controlling oil and resisting inflammation.

[0053] Table 1 shows the composition ratio of each composition in Examples 1-10 and Comparative Examples 1-3 (the composition ratio of the lotus seed extract, the Phellinus linteus extract and the Myrtus communis fruit extract is by mass). According to the composition and ratio shown in Table 1, the compositions of each example and comparative example are prepared by using the above method, and the obtained compositions are subjected to efficacy test.

[0054] Table 1 shows the composition ratio of each composition in Examples 1-10 and Comparative Examples 1-3 (the composition ratio of the lotus seed extract, the Phellinus linteus extract and the Myrtus communis fruit extract is by mass). According to the composition and ratio shown in Table 1, the compositions of each example and comparative example are prepared by using the above method, and the obtained compositions are subjected to efficacy test.

[0055]

[0056]

[0057] Experimental Example 1

[0058] Cytotoxicity evaluation test:

[0059] MTT is a yellow powder chemical reagent, which is widely used in the detection of cytotoxicity or cell proliferation. The detection principle is that succinate dehydrogenase in the mitochondria of living cells can reduce water-soluble yellow salt MTT (3-(4, 5-dimethylthiazole-2)-2, 5-diphenyl tetrazolium bromide) to water-insoluble blue formazan, which is deposited in the cells, while dead cells have no such function. The generated formazan is generally dissolved in DMSO (dimethyl sulfoxide) after which the absorbance is measured. The specific experimental method is to inoculate 100 μl of DMEM medium containing 10% bovine serum and HaCaT cells in a 96-well plate at a density of 1X10 4 After 24 hours of culture, the medium was replaced with serum-free medium. After 24 hours of culture in serum-free medium, different concentrations of the composition were added for treatment. Then the medium was removed, 20 μl of MTT solution was added for treatment, and the reaction was allowed to proceed at 37°C for 4 hours. 200 μl of isopropyl alcohol was added to the cells from which the MTT solution was removed, and the mixture was gently shaken for 30 min to completely dissolve the formazan. The absorbance was measured at 570 nm, and the cell survival rate was calculated according to the following formula.

[0060]

[0061] The blank group did not add the composition for testing. Table 2 shows the cytotoxicity results of the compositions obtained in Examples 1-10 and Comparative Examples 1-3.

[0062] Table 2 Cytotoxicity results of the compositions obtained in Examples 1-10 and Comparative Examples 1-3

[0063]

[0064]

[0065] Experimental Example 2

[0066] Based on Raw264.7 cell anti-inflammatory test:

[0067] TNF-α, IL-6 are inflammatory factors, and the ELISA method is used to detect the inhibition of TNF-α, IL-6 inflammatory factors in the blank group, the lipopolysaccharide (LPS) induced Raw264.7 cell after modeling group, the TNF-α, IL-6 content after 2% wt concentration of Example 3, Example 7, Example 8, Example 9, Comparative Examples 1-3 in culture, and the anti-inflammatory effect of the composition is detected. After stimulating Raw264.7 cells with 4 μg / mL LPS diluted from 1 mg / mL LPS stock solution, the sample to be tested (10X) is added to the 96-well cell plate at 100 μl per well, and incubated in a 37°C 5% CO2 constant temperature incubator for 72 h. Each cell plate sets up a negative control group (cells + medium), and each concentration group has 3 replicate wells. The cell culture supernatant is collected, and the anti-inflammatory efficacy is evaluated by ELISA kit. The test results are shown in Table 4 and Table 5. Figure 1 and Figure 2 The content detected by the blank group is taken as the reference amount.

[0068] The results show that Examples 8-9 can well inhibit the production of inflammatory factors after LPS stimulation, and have significant anti-inflammatory effect, and the anti-inflammatory effect is better than that of Example 3, Example 7, Comparative Examples 1-3.

[0069] Experimental Example 3

[0070] Safety patch test

[0071] 0.020 mL of product is placed in the patch tester, and the control hole is blank control (pure water); the patch tester with the test substance is applied to the flexor of the forearm of the test subject with non-irritating tape, and the palm is lightly pressed to make it evenly adhere to the skin for 24 hours; the skin irritation and sensitization are observed at 30 min, 24 h, and 48 h after the test substance patch tester is removed, respectively, according to Table 3, and the observation results are recorded. The skin reaction grading standard of the skin occlusive patch test is shown in Table 3.

[0072] Table 3 Skin reaction grading criteria for patch test

[0073]

[0074] Experimental results: The compositions obtained in Examples 5 and 8 were subjected to human skin patch test, and the results are shown in Table 4. The results of the human skin patch test showed that there was no skin adverse reaction in 30 people.

[0075] Table 4

[0076]

[0077] Experimental Example 4

[0078] Thirty-two healthy volunteers were screened, 16 males and 16 females, aged 18-45 years. The amount of skin surface sebum was detected by a skin surface sebum tester Sebumeter at 0 h before product use, 2 h and 4 h after product use, respectively, using the emulsion containing 2% (by mass) of the composition of Example 3, Example 7 and the compositions of Comparative Examples 1-3. The higher the sebum measurement value, the higher the amount of skin surface sebum.

[0079] Experimental results: As shown in Figure 3 , the skin oil content of Examples 8-9 was lower than that of Example 3, Example 7 and Comparative Examples 1-3 after 2 hours and 4 hours of use, indicating that the composition of Examples 8-9 has a more excellent oil control effect than the combination of two components.

[0080] Experimental Example 5

[0081] Thirty-two healthy volunteers were screened, 16 males and 16 females, aged 18-45 years. The emulsion containing 2% (by mass) of the composition of Example 3, Example 7, Example 8, Example 9 and Comparative Examples 1-3 was used once a day in the morning and evening, for 4 weeks. The trans-epidermal water loss rate was detected by a trans-epidermal water loss tester Tewameter TM HEX, and the erythema index a* value was measured and analyzed by a skin color test probe Colorimeter CL400. The results are shown in Figure 4 and Figure 5 .

[0082] It is easily understood by those skilled in the art that the above description is only the preferred embodiment of the present application, and is not intended to limit the present application. Any modification, equivalent replacement and improvement made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A composition having oil-controlling and anti-inflammatory effects, characterized in that, The composition with oil-controlling and anti-inflammatory effects includes lotus seed extract, medicinal fusiforme extract and myrtle fruit extract. The mass ratio of lotus seed extract, medicinal blastocystis extract and myrtle fruit extract is (1-6):(0.05-0.2):(1-6); The preparation method of the lotus seed extract includes the following steps: 1) Select crushed germinated lotus seeds and ferment them at room temperature for 3-6 days; 2) Add 10-20 times the amount of polyol used for germinating lotus seeds for extraction. The extraction temperature is 45-65℃, the extraction time is 3-5 hours, and the extraction is repeated 1-2 times. 3) Cool the extract to 25°C or below and filter it through 100-200 mesh gauze to obtain the initial filtrate; 4) Allow the primary filtrate to stand, centrifuge, and filter to obtain the secondary filtrate; 5) Concentrate the secondary filtrate under reduced pressure to 1 / 4-1 / 2 of its original weight, add 1-10 times the weight of the concentrate of deionized water, mix well, filter, collect the filtrate, and obtain lotus seed extract. The preparation method of the medicinal Fomitopsis pinnatifida extract includes the following steps: 1) Select dried medicinal fumonis, pulverize and sieve to obtain medicinal fumonis powder; 2) Add 1-10 times the amount of deionized water as the obtained medicinal fumonis powder, extract 1-3 times at a temperature of 45-60℃, and collect the extract; 3) Cool the extract obtained in the previous step to 20-30℃ and filter to obtain the initial filtrate; 4) Centrifuge and filter the initial filtrate obtained in the previous step, collect the filtrate, and obtain the medicinal Fomitopsis pilosula extract; The preparation method of the myrtle fruit extract includes the following steps: 1) Select myrtle fruit and soak it in 10-20 times the amount of polyol for 4-6 hours; 2) Extraction was performed using ultrasonic vibration, followed by filtration and collection of the filtrate; 3) Add clarifying agent, let stand at low temperature for 3-5 hours, then filter; 4) Concentrate the filtrate under reduced pressure at a temperature of 50-60℃ to obtain a concentrated solution; 5) Add 10-20 times the weight of the concentrate in propylene glycol, mix well, filter, collect the filtrate, and obtain myrtle fruit extract.

2. The composition with oil-controlling and anti-inflammatory effects according to claim 1, characterized in that, The mass ratio of lotus seed extract, medicinal fusiforme extract and myrtle fruit extract is (1-4):(0.05-0.1):(1-4).

3. The composition with oil-controlling and anti-inflammatory effects according to claim 1, characterized in that, The mass ratio of lotus seed extract, medicinal fusiforme extract and myrtle fruit extract is 4:(0.05-0.1):(3-4).

4. The composition with oil-controlling and anti-inflammatory effects according to claim 1, characterized in that, The mass ratio of lotus seed extract, medicinal blastocystis extract, and myrtle fruit extract is 4:0.05:3 or 4:0.1:

4.

5. A method for preparing the composition with oil-controlling and anti-inflammatory effects according to any one of claims 1-4, characterized in that, The method involves weighing lotus seed extract, medicinal blastocystis extract, and myrtle fruit extract according to a certain ratio, and mixing them thoroughly.

6. The method for preparing the composition with oil-controlling and anti-inflammatory effects according to claim 5, characterized in that, In the preparation method of the composition with oil-controlling and anti-inflammatory effects, a solvent may be added before mixing lotus seed extract, medicinal blastocystis extract and myrtle fruit extract.

7. The method for preparing the composition with oil-controlling and anti-inflammatory effects according to claim 6, characterized in that, The solvent is one or more of water, butanediol, glycerol, propylene glycol, and ethanol.

8. A cosmetic product, characterized in that, The cosmetic contains the composition with oil-controlling and anti-inflammatory effects as described in any one of claims 1-4.

9. The cosmetic product according to claim 8, characterized in that, The cosmetics mentioned are aqueous solutions, emulsions, sprays, creams, masks, or foundations.

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