Application of 3-decanone in repelling solenopsis invicta

Abstract

The invention discloses an application of 3-decanone in repelling red imported fire ants. According to the method, the chemical substance 3-decanone separated from the bootstrap abdominal ant is used for repelling the solenopsis invicta. Experimental results show that after 3-decanone with the concentration of 10 microliters / mL or above is treated for 1.5 h, the repelling rate of solenopsis invicta reaches 86% or above; and after 12 hours of treatment, a relatively high repelling effect can still be maintained. The 1, 3-decanone has a good repelling effect on solenopsis invicta, can keep certain repelling activity after a long time, is an efficient and environment-friendly compound, and can be used as a solenopsis invicta repellent in the future.

Description

Technical Field

[0001] The invention belongs to the technical field of prevention and treatment of red imported fire ants, and particularly relates to application of 3-decanone in repelling red imported fire ants. Background Art

[0002] Red fire ants (Solenopsis invicta Buren) are listed as one of the important invasive species in my country. They can cause serious harm to public safety, human health, agricultural and forestry production and the ecological environment. The feeding range of red fire ants includes: the nutritional organs of crops, other local insects, etc., which seriously threatens local biodiversity. At the same time, red fire ants can also cause great harm to crops by grazing aphids and other insects that produce honeydew. Red fire ants are extremely aggressive and will launch a fierce attack on the disturbers when their nests are disturbed. After being bitten by red fire ants, the human body will feel a burning pain, and blisters like burns will appear about one day after being bitten. In the areas where red fire ants occur in my country, about 30% of the population have experienced being stung by red fire ants, of which 10% of the victims have fever symptoms after being stung, a small number of people have allergic reactions such as dizziness, itching, and edema, and very few have shock symptoms, which seriously affects people's work and life.

[0003] So far, a variety of insecticides have been used to control red fire ants, and more than 30 contact pesticides have been registered in China for the control of red fire ants. However, some ecologically sensitive areas such as nature reserves, organic agricultural land, and indoor places such as medical education are not suitable for control with insecticides. In recent years, the concept of green agriculture has appeared more and more frequently, and the green control technology of red fire ants has also attracted more and more attention from researchers. More and more researchers are focusing on biological control directions such as natural plants and insects that compete with red fire ants, in order to develop new and environmentally friendly control methods. Although insecticides can successfully inhibit the spread of red fire ants, they cannot take effect quickly. Therefore, screening out compounds with repellent activity against red fire ants has received more and more attention. However, in my country, ant repellent research started late, and the existing insect repellents on the market are mostly for sanitary pests such as mosquitoes, flies, and mites. At present, almost no repellents for red fire ants have been commercialized and put into use.

[0004] One of the reasons why the currently effective repellents have not been commercialized is that they may have potential environmental hazards. The second reason is that most effective red fire ant repellents can only be effective for a short period of time, and their repellent effect lasts for a short time. Therefore, the development of effective and long-lasting red fire ant repellents is an important direction at present. Summary of the invention

[0005] The purpose of the present invention is to provide timely and effective protection, minimize the health hazards of red fire ants to humans and animals, develop a safe, timely, long-lasting red fire ant repellent with safe active ingredients and low environmental hazards, and apply it to daily protective measures.

[0006] The first object of the present invention is to provide the use of 3-decanone in repelling red fire ants.

[0007] Preferably, the red fire ants are worker ants of the red fire ant.

[0008] Preferably, the application is an application for repelling feeding of red fire ants and / or repelling digging behavior of red fire ants.

[0009] Preferably, the use comprises the following steps: applying 3-decanone to the area where red fire ants are to be repelled.

[0010] Preferably, the 3-decanone is used as an active ingredient for repelling red fire ants, and the concentration of 3-decanone is above 5 μL / mL.

[0011] Preferably, the 3-decanone is used as an active ingredient for repelling red fire ants, and the concentration of 3-decanone is above 10 μL / mL.

[0012] The second object of the present invention is to provide a red fire ant repellent, wherein 3-decanone is used as an active ingredient in the red fire ant repellent, and the concentration of 3-decanone in the red fire ant repellent is above 5 μL / mL.

[0013] Beneficial effects of the present invention:

[0014] The 3-decanone used for repelling red fire ants of the present invention can be separated from the black brown ant. As a natural extract, it is very environmentally friendly. Experiments have shown that the 3-decanone of the present invention can remain effective after 12 hours, with a long lasting effect, and the repellency rate of red fire ants can reach more than 96%. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a partial chromatographic peak diagram of the volatile sample of the black brown ant; among them: Peak 1 is 4-nonanone, Peak 2 is 3-nonanone, and Peak 3 is 3-decanone.

[0016] Figure 2 is a diagram of the experimental setup; wherein: a is the setup used in the feeding repellent experiment, and b is the setup used in the digging repellent experiment.

[0017] Figure 3 This is a graph showing the results of measuring the feeding repellent activity of 3-decanone against red imported fire ants.

[0018] Figure 4This is a graph showing the results of determining the repellent activity of 3-decanone on the digging behavior of red imported fire ants; different letters indicate significant differences between treatments (p<0.05). DETAILED DESCRIPTION

[0019] The following examples are provided to further illustrate the present invention, rather than to limit the present invention.

[0020] The 3-decanone used in the following examples was purchased from Shanghai MacLean Biochemical Technology Co., Ltd. with a purity of 98% and a dosage form of soluble solution; acetone was purchased from Guangdong Guangshi Reagent Technology Co., Ltd. with a purity of ≥99.5%.

[0021] Example 1

[0022] 1. Materials and Methods

[0023] 1.1 Isolation and identification of 3-decanone

[0024] To determine the volatile substances in the venom of the black-brown ant, about 200 black-brown ant workers were placed in a 250 mL triangular flask, sealed with a sealing film, and a layer of tin foil was wrapped around the mouth of the flask. The black-brown ant in the flask was disturbed by shaking to make it in an active attack state until the tail secreted venom droplets hanging on the stinger. The sample was immediately subjected to headspace solid phase microextraction (HS-SPME) at room temperature (26±1℃) for 30 minutes using a 57310-U manual solid phase microextraction device and a 65μm PDMS / DVB extraction fiber head. Before the sample measurement, the instrument was blanked and the fiber was cleaned in the GC injector for 30 minutes.

[0025] GC conditions: HP-5MS elastic quartz capillary column (30m×0.25mm×0.25μm). Inlet temperature: 280℃, helium as carrier gas, carrier gas flow rate: 1.00mL / min. Column heating program: initial temperature 50℃, hold for 2min, increase to 280℃ at 10℃ / min, hold for 5min. No split, injection volume 1μL. MS conditions: EI ionization mode, ion source temperature 230℃, electron energy 70eV, quadrupole temperature 150℃, mass scanning range 35~550m / z. Manual analysis of the spectrum, matching the obtained mass spectrum with the NIST.14 spectral library search, compound identification, and area normalization method to obtain the relative content of each component.

[0026] 1.2 Repellency test of 3-decanone on red imported fire ants

[0027] 1.2.1 Food repellency experiment

[0028] To determine the repellent effect of 3-decanone on the feeding of red imported fire ants, a self-made device 1 (such as Figure 2a) in the test. Take two plastic boxes as the living area and the feeding area respectively, connect them with a connecting tube, and coat the edge of the box with polytetrafluoroethylene to prevent the escape of red fire ants. Before the experiment, the experimental red fire ant workers (collected from Huadu District, Guangzhou City, Guangdong Province) were starved for 12 hours. At the beginning of the experiment, 3±0.1g of experimental red fire ant workers were weighed and placed in the living area of ​​the device to adapt to the unfamiliar environment, and then 1±0.1g of ham sausage (Shuanghui, Henan Shuanghui Investment Development Co., Ltd.) was weighed and placed in the middle of a 7cm filter paper, and 600μL of the agent was dripped on the filter paper. The solvent of the agent is acetone with a concentration of ≥99.5%, and the solute is 3-decanone. Among them, in the set agent, the concentration gradient of 3-decanone is 0.1μL / mL, 1μL / mL, and 10μL / mL, and the dripping of acetone with a concentration of ≥99.5% is used as a control. Filter papers soaked with different concentration gradient agents were placed in the feeding area 10 cm away from the pipe opening of the area, and the position of the filter papers was adjusted to make the distance between adjacent filter papers equal. At the beginning of the experiment, the cover of the small connecting pipe was opened and the timing was started. The number of foraging red fire ant workers on the filter paper was observed and recorded every 0.5 hours, and the observation and recording was carried out for a total of 1.5 hours.

[0029] 1.2.2 Digging repellent experiment

[0030] To determine the repellent effect of 3-decanone on the digging behavior of red imported fire ants, a self-made device 2 (such as Figure 2 b) in the test. Take a 14 cm diameter culture dish, evenly apply polytetrafluoroethylene on the inner wall of the culture dish to prevent the red fire ants from escaping, fix a 5 mL sample bottle (purchased from Haimen Zhongrui Experimental Equipment Business Department) at 4 cm from the center of the circle on two vertical diameters at the bottom, a total of 4, use a small electric drill to drill a small hole from the side of the culture dish to the side of the sample bottle (enough for the red fire ants to pass through), and connect the culture dish and the sample bottle.

[0031] Quartz sand (20-40 mesh, Tianjin Huasheng Chemical Reagent Co., Ltd.) was dried to constant weight, and then 5 mL of the reagent was poured into 50 g of quartz sand and stirred evenly for 5 minutes, and then 5 mL of distilled water was added, and stirred evenly for 5 minutes to fully mix, and then stood for 5 minutes for standby. Among them, the solvent of the reagent is acetone with a concentration of ≥99.5%, and the solute is 3-decanone. The concentration gradient of 3-decanone is set to: 5 μL / mL, 10 μL / mL, 20 μL / mL, and acetone with a concentration of ≥99.5% is used as a control. Quartz sand treated with different reagents (5 μL / mL, 10 μL / mL, 20 μL / mL of 3-decanone, and acetone with a concentration of ≥99.5% is used as a control) was loaded into each sample bottle, and the weight of the quartz sand treated with the reagents was 3±0.1 g. Finally, 50 red fire ant workers for the experiment were placed in the culture dish, and the device 2 was placed in a dark place for 24 hours. The sample bottles were then removed and dried, and the weight of the remaining quartz sand in each bottle was weighed and recorded.

[0032] 1.3 Data processing and analysis

[0033] In the feeding repellent experiment, R = [(CT) / (C+T)] × 100%; R represents the repellent rate; C represents the number of insects staying on the control filter paper; T correspondingly represents the number of insects staying on the treated filter paper.

[0034] In the excavation and repellent experiment, ΔD=Di-Dp; ΔD represents the excavation amount, Di represents the mass of the initial quartz sand, and Dp represents the weight of the remaining quartz sand.

[0035] The Shapiro-Wilk test was used to test the normal distribution of the data of the number of red fire ant workers (T) and the amount of quartz sand excavation (ΔD) in the treatment area. The data that met the normal distribution were tested for homogeneity of variance using one-way analysis of variance. For homogeneity of variance, one-way analysis of variance was used, and multiple comparisons were performed using Tukey test. For those that did not meet the normal distribution and had unequal variance, the Kruskal-Wallis test was used. All data analysis programs were run on SPPSS27.0.

[0036] 2. Results

[0037] 2.1 Analysis of GC-MS results

[0038] The qualitative analysis of some compounds was obtained by comparing the chromatographic mass spectrum obtained by GC-MS with the spectral library. The peaks, molecular formulas and relative contents of the target compounds are shown in Table 1. The chromatogram of the standard sample of 3-decanone is shown in Figure 1 3 of 3.

[0039] Table 1 Analysis of some components of volatile samples of the black brown ant

[0040] Peak position / min Molecular formula Molecular weight Compound Relative content (%) 10.03 <![CDATA[C 10 H 20 O]]> 156.27 3-Decanone 0.38

[0041] 2.2 Repellent activity of 3-decanone preparation dilutions

[0042] Under indoor conditions, the feeding and digging repellent activities of 3-decanone at different concentrations against red imported fire ants were determined. The experimental setups were as follows: Figure 2 a in Figure 2 As shown in b.

[0043] In the feeding avoidance experiment (such as Figure 3), at the same observation time, after 0.5h treatment, the number of foraging red fire ant workers in the 10μL / mL concentration area was significantly lower than that in the control area (CK) and other concentration areas (df=35, P<0.01), and the feeding repellent rate reached more than 86%. There was no significant difference between the number of foraging red fire ant workers in the 1μL / mL and 0.1μL / mL concentrations and the control area (1μL / mL, df=35, P=0.141; 0.1μL / mL, df=35, P=0.795); at 1h and 1.5h treatment, the experimental results did not change significantly, and the 10μL / mL agent had the best effect. (Shapiro-Wilk test, 0.5h, df=12, P=0.278; 1h, df=12, P=0.228, 1.5h, df=12, P=0.336). At the same concentration, there was no significant difference in the repellent effect with time. After 12 hours of treatment, it was observed that the number of foraging red fire ant workers in the 10 μL / mL concentration area was significantly lower than that in the control area (df=11, F=17.202, P=0.02), and the repellent rate reached 96%.

[0044] In the digging repellent experiment (e.g. Figure 4 ), after 24 hours, a large amount of quartz sand was excavated in the control group (CK), while the excavated amount of quartz sand in the treatment group (5μL / mL, 10μL / mL, 20μL / mL of 3-decanone) was significantly lower than that in the control group (20μL / mL, df=7, F=70.415, P<0.01; 10μL / mL, df=7, F=70.023, P<0.01; 5μL / mL, df=7, F=69.856, P<0.01). There was no significant difference in the excavated amount between the treatment groups (df=11, F=0.157, P=0.857).

[0045] The above results show that after being treated with 3-decanone at a concentration of 10 μL / mL or more for 1.5 hours, the feeding repellency rate of red fire ant workers reached 86%; after 12 hours of treatment, it still maintained a good repellent effect (repellent rate 96%), indicating that 3-decanone has a long-lasting repellent activity against red fire ants. After being treated with 3-decanone at a concentration of 5 μL / mL or more for 24 hours, the digging habits of red fire ant workers can be significantly reduced. 3-decanone at a concentration of 10 μL / mL or more has a good repellent effect on red fire ants and can be used in red fire ant protective agents in the future; 3-decanone at a concentration of 5 μL / mL or more can be used as a soil repellent for red fire ants.

Claims

Application of 1.3-decanone in repelling red fire ants.

2. The use according to claim 1, characterized in that: The red fire ants are worker ants.

3. The use according to claim 1, characterized in that: Application for repelling feeding of red fire ants and / or repelling digging behavior of red fire ants.

4. The use according to claim 1, characterized in that: The following steps are involved: 3-Decanone is applied to the area to be repelled from red fire ants.

5. The use according to claim 1, characterized in that: The 3-decanone is used as an active ingredient for repelling red fire ants, and the concentration of the 3-decanone is above 5 μL / mL.

6. The use according to claim 5, characterized in that: The 3-decanone is used as an active ingredient for repelling red fire ants, and the concentration of the 3-decanone is above 10 μL / mL.

7. A red fire ant repellent, characterized in that: 3-Decanone is used as an active ingredient in the red fire ant repellent, and the concentration of 3-decanone in the red fire ant repellent is above 5 μL / mL.

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