Traditional Chinese medicine fumigation-washing formula for treating peripheral ankylosing spondylitis

Abstract

A traditional Chinese medicine fumigation and washing prescription for treating peripheral ankylosing spondylitis belongs to the field of traditional Chinese medicine preparations for treating peripheral ankylosing spondylitis, and the traditional Chinese medicine fumigation and washing prescription comprises radix aconiti lateralis praeparata, radix paeoniae alba, cornu cervi pantotrichum, herba epimedii, radix salviae miltiorrhizae, pseudo-ginseng, radix stephaniae tetrandrae, herba siegesbeckiae, tripterygium hypoglaucum, pepper, curcuma zedoary, trogopterus dung, radix sophorae flavescentis and semen cuscutae. Fumigation is performed in a water vapor manner, and the efficacy is lasting and mild. The whole formula has the effects of warming kidney to strengthen yang, promoting blood circulation to remove blood stasis, dispelling wind and eliminating dampness, and activating meridians to stop pain.

Description

Technical Field

[0001] The invention belongs to the field of traditional Chinese medicine preparations for treating peripheral ankylosing spondylitis, and specifically relates to a traditional Chinese medicine fumigation and washing prescription for treating peripheral ankylosing spondylitis. Background Art

[0002] Ankylosing spondylitis belongs to the category of "kyphosis" in traditional Chinese medicine. The etiology and pathogenesis of kyphosis are relatively complex. Liver and kidney deficiency and insufficient qi and blood are the internal causes of this disease, and exposure to wind, cold and dampness are its inducing factors. Insufficient congenital endowment, weak constitution, insufficient liver and kidney essence and blood, and empty kidney governor. Wind, cold and dampness take advantage of the weakness to deeply invade the kidney governor and stay in the meridians and joints, blocking qi and blood. Or the course of the disease is long, qi and blood are insufficient, and the circulation of qi, blood and body fluids is weak, or cold and dampness are long-term and turn into heat, and the evil of wind, cold, dampness and heat stay in the meridians and joints, affecting the circulation of qi, blood and body fluids, leading to phlegm and blood stasis, and muscle spasm and bone damage, joint deformity, and back stiffness and disuse. Liver and kidney deficiency, qi and blood deficiency, cold and dampness, phlegm and blood stasis blocking the meridians are the key to the pathogenesis. Therefore, the treatment of kyphosis in traditional Chinese medicine should focus on warming the kidney and tonifying yang, promoting blood circulation and removing blood stasis, and unblocking meridians and relieving pain. Summary of the invention

[0003] The purpose of the present invention is to provide a Chinese medicine fumigation and washing prescription for effectively treating peripheral ankylosing spondylitis. The effective components of the Chinese medicine are extracted by heating, and the medicine is fumigated in the form of water vapor. The medicinal effect is long-lasting and mild, and the effects of warming the kidney, replenishing yang, and promoting blood circulation and removing blood stasis are achieved.

[0004] The technical solution adopted by the present invention is:

[0005] A Chinese medicine fumigation and washing prescription for treating peripheral ankylosing spondylitis, wherein the effective ingredients are prepared from the following Chinese medicine raw materials by weight:

[0006] Processed Aconite 5-15g, White Peony 5-10g, Deer Antler 5-15g, Epimedium 5-15g, Salvia miltiorrhiza 5-10g, Panax notoginseng 5-15g, Stephania tetrandra 5-15g, Siegesbeckia 5-15g, Malus 5-15g, Zanthoxylum bungeanum 5-10g, Curcuma 5-15g, Notopterygium wilfordii 5-10g, Sophora flavescens 5-10g, Cuscuta australis 5-15g.

[0007] Paofuzi, deer antler, epimedium, and dodder are warm-natured medicines, which play a role in warming and tonifying kidney yang in the formula. Modern pharmacological studies have found that the water-soluble extract of aconite can enhance humoral immunity, enhance the expression level of antigens on the surface of macrophages and improve their antigenic capacity, and promote the body's immune response. Some scholars have found through mouse foot swelling tests that deer antler collagen hydrolysate has an inhibitory effect on carrageenan-induced foot swelling. Deer antler polypeptides and deer antler polysaccharides can antagonize osteoporosis in rats, and deer antler alcohol extracts and water extracts can significantly increase the length and bone density of trabeculae in osteoporotic mice, increase ALP activity and blood calcium levels, and enhance anti-osteoporosis effects. Icaritin can regulate the production of inflammatory factors and interfere with the function of immune cells through multiple pathways and targets, and has low clinical toxicity. Cuscuta total extract and Cuscuta polysaccharide can effectively correct the imbalance of Th1 / Th2 inflammatory factors, inhibit the secretion of pro-inflammatory factor TNF-α, and upregulate the level of anti-inflammatory factor IL-10, thereby alleviating the inflammatory response.

[0008] White peony root, salvia miltiorrhiza, Panax notoginseng, Curcuma zedoaria, and Rhizoma Atractylodis Macrocephalae are all blood-activating and blood-nourishing herbs. They play a role in promoting blood circulation and unblocking collaterals in the formula. White peony root has pharmacological effects such as analgesia and immune regulation. White peony root total glucosides are the main active ingredients of white peony root, which can regulate the function of immune cells and balance immune cell subsets. White peony root decoction can effectively alleviate the clinical symptoms of arthritis patients, increase anti-inflammatory cells Treg by regulating the NF-κB signaling pathway, inhibit the differentiation of T cells into Th17 cells, delay the process of joint damage, and enhance immune regulation. Salvianolic acid, a water-soluble component of salvia miltiorrhiza, can significantly reduce the expression of inflammatory factors such as TNF-α and IL-1β, thereby effectively inhibiting the inflammatory response. In addition, salvianolic acid can further reduce the inflammatory response by inhibiting the release of inflammatory mediators, such as PGE2 and the production of oxygen free radicals. Some scholars used tanshinone extract to treat rat arthritis models. The results showed that tanshinone extract can regulate the expression of SOD, MDA, IL-6, and TNF-α in the serum in vivo, thereby playing an anti-inflammatory role and improving the condition. Studies have shown that Protocatechuic acid, Caffeic acid, Quercetin and Luteolin in Panax notoginseng have significant inhibitory effects on anti-inflammatory factors such as NO, TNF-α and IL-6. The volatile oil of Curcuma zedoaria can effectively reduce the protein expression levels of serum inflammatory factors IL-2, hs-CRP and TNF-α, and has significant anti-inflammatory and antibacterial activities. The ethyl acetate extract of Penicillium truncatum can significantly reduce the PGE content in inflammatory tissues.

[0009] Stephania tetrandra, Herba Siegesbeckiae, and Herba Kunmingii are all drugs that can dispel wind and dampness and relieve pain. The immunomodulatory effect of tetrandra tetrandra is mainly manifested in inhibiting the proliferation and activation of inflammatory cells such as T lymphocytes, neutrophils, and mononuclear macrophages, resisting free radical damage, and inhibiting the release of inflammatory mediators. Herba Siegesbeckiae and its extracts have an inhibitory effect on pain. Some scholars found in the mouse heat pain reaction test that when Herba Siegesbeckiae was applied externally, the number of mouse twistings gradually decreased with the increase of Herba Siegesbeckiae concentration, indicating that Herba Siegesbeckiae has anti-inflammatory and analgesic effects. For Herba Kunmingii, it also has the effect of inhibiting inflammatory response. In the mouse model of plantar edema, the compound Herba Kunmingii granules can significantly reduce the plantar edema of rats.

[0010] Patients with ankylosing arthritis have different constitutions. In order to avoid allergies in patients with sensitive skin, pepper and sophora flavescens are added to the prescription. Pepper volatile oil can effectively relieve chronic localized skin itching and discomfort, and has high safety. Pepper volatile oil and its microemulsion gel have a good penetration-promoting effect on both hydrophilic and lipophilic drugs, which can promote the penetration of traditional Chinese medicine and reduce skin irritation, making the drug effect synergistic. Sophora flavescens can relieve itching and has a certain effect on skin itching. In addition, matrine can also relieve muscle atrophy and improve myoblast differentiation by inhibiting E3 ubiquitin ligase and activating Akt / mTOR / FoxO3α signaling pathway, and has a certain anti-inflammatory effect.

[0011] In the fumigation prescription proposed by the present invention, the roasted aconite is warm in nature, and plays the role of replenishing fire and yang, dispelling cold and removing dampness; the antler of deer antler replenishes kidney yang and strengthens tendons and bones. The "Compendium of Materia Medica" records: "Deer antler tastes sweet, sour, warm, slightly warm, non-toxic, and is mainly used to treat asthenia such as malaria, emaciation, sore limbs, and back pain." Cuscuta chinensis replenishes kidney yang and strengthens tendons and bones, and is the main drug together with epimedium and deer antler. Salvia miltiorrhiza, Panax notoginseng, Curcuma zedoaria, and Atractylodes macrocephala all have the function of promoting blood circulation, and Curcuma zedoaria can break blood with a stronger force, and together they play the functions of promoting blood circulation, unblocking collaterals, removing blood stasis and relieving pain, and are the minister drugs. Stephania tetrandra, Herba Siegesbeckiae, and Herba Hibisci Kunmingensis are all anti-rheumatic drugs. Stephania tetrandra dispels wind and relieves pain, promotes diuresis and reduces swelling; Herba Siegesbeckiae dispels wind and dampness, promotes blood circulation and activates collaterals; the three herbs work together to remove dampness, promote blood circulation and relieve pain, and are the guiding herbs; "Shennong's Herbal Classic" says: "Peony root is bitter and flat in taste. It is used to treat abdominal pain caused by evil qi, remove blood stasis, break up hard masses, cold and heat, hernia, relieve pain, promote urination, and invigorate qi", and it plays the role of nourishing blood, restraining yin, softening the liver and relieving pain in the prescription. It is supplemented with Radix Sophorae Flavescentis and Zanthoxylum bungeanum to dispel wind and relieve itching, warm the middle and relieve pain; the whole prescription has the functions of warming the kidney and supporting yang, promoting blood circulation and removing blood stasis, dispelling wind and dampness, and promoting blood circulation and relieving pain.

[0012] As a preferred technical solution of the present invention, the effective ingredients of the Chinese medicine fumigation and washing prescription are prepared from the following Chinese medicine raw materials by weight:

[0013] 10g of cooked aconite root, 8g of white peony root, 10g of deer antler, 10g of epimedium, 8g of salvia miltiorrhiza, 10g of Panax notoginseng, 10g of Stephania tetrandra, 10g of Siegesbeckia herb, 10g of Kunming crabapple, 6g of Zanthoxylum bungeanum, 10g of Curcuma zedoaria, 8g of Notopterygium wilfordii, 6g of Sophora flavescens, and 10g of Cuscuta australis.

[0014] The specific preparation method is: mix the above medicinal materials, add 3-5 times the weight of water, soak for 30 minutes, and then boil for 30 minutes; fumigate with water vapor when using. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 Comparison of TCM syndrome scores between the two groups before and after treatment (*p<0.05, **p<0.01).

[0016] Figure 2 Comparison of knee joint function scores between the two groups before and after treatment (**p<0.01, ****p<0.0001). DETAILED DESCRIPTION

[0017] The following is a further detailed description of a traditional Chinese medicine fumigation and washing prescription for treating peripheral ankylosing spondylitis of the present invention in conjunction with the accompanying drawings and examples.

[0018] Example 1

[0019] The Chinese medicine fumigation and washing recipe is prepared from the following Chinese medicine raw materials by weight:

[0020] 10g of cooked aconite root, 8g of white peony root, 10g of deer antler, 10g of epimedium, 8g of salvia miltiorrhiza, 10g of Panax notoginseng, 10g of Stephania tetrandra, 10g of Siegesbeckia herb, 10g of Kunming crabapple, 6g of Zanthoxylum bungeanum, 10g of Curcuma zedoaria, 8g of Notopterygium wilfordii, 6g of Sophora flavescens, and 10g of Cuscuta australis.

[0021] Mix the above medicinal materials, add 630g of water, soak for 30 minutes, and then boil for 30 minutes; fumigate with water vapor when using.

[0022] Example 2

[0023] In this experiment, an ankylosing spondylitis mouse model was prepared to study the effects of Chinese medicine fumigation on TCM syndromes and knee joint function in model mice.

[0024] 1. Materials and Methods

[0025] 1.1 Animals

[0026] Twenty SPF male mice, aged 42 to 56 days, were purchased from Anhui Experimental Animal Research Center (License No.: SYXK(Wan)2023-005).

[0027] 1.2 Reagents

[0028] Bovine type II collagen (Chondrex), Freund's incomplete adjuvant (Chondrex), Freund's complete adjuvant (Sigma).

[0029] 1.3 Traditional Chinese medicine fumigation prescription

[0030] Chinese medicine fumigation formula: 10g of roasted aconite, 8g of white peony root, 10g of pilose antler, 10g of epimedium, 8g of salvia miltiorrhiza, 10g of Panax notoginseng, 10g of Stephania tetrandra, 10g of Siegesbeckia sibiricum, 10g of Kunming Mountain Begonia, 6g of Zanthoxylum bungeanum, 10g of Curcuma zedoaria, 8g of Pennisetum officinale, 6g of Sophora flavescens, and 10g of Cuscuta australis. This is the agreed formula of the Department of Rheumatology and Immunology of the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, and the Chinese medicinal materials used are provided by the Chinese medicine pharmacy of the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine. Mix the above medicinal materials, add 630g of water, soak for 30min, and then boil for 30min; fumigate with water vapor when using.

[0031] 1.4 Methods

[0032] 1.4.1 Modeling

[0033] On days 0, 21, and 42 of modeling, 200 μL of a solution containing 100 μg of proteoglycan emulsifier (100 μL PBS: 100 μL complete Freund's adjuvant) was injected intraperitoneally to induce ankylosing spondylitis mouse model.

[0034] 1.4.2 Administration

[0035] The control group was given methotrexate tablets (Shanghai SPH Xinyi Pharmaceutical Co., Ltd., National Medicine Approval No. H31020644), 10 mg / time, once a week by gavage; the research group was given a high-concentration Chinese medicine fumigation prescription on the basis of the control group, once a day for 6 weeks. The various indicators of the rats were observed every day during the six weeks.

[0036] 1.4 Observation indicators and detection methods

[0037] (1) Compare the TCM syndrome scores of the two groups before and after treatment. The higher the score, the more severe the symptoms.

[0038] (2) The knee joint function of mice before and after treatment was evaluated using the knee joint function assessment table. The full score is 100, and the higher the score, the better.

[0039] 2. Results

[0040] 2.1 Comparison of TCM Syndromes

[0041] The changes in toe volume of mice in each group were compared before and after 6 weeks of Chinese medicine fumigation. The scores of joint swelling, flexion and extension difficulties, and joint pain in the control group before treatment were (5.58±0.45) points, (5.41±0.17) points, and (5.31±0.12) points; the scores of joint swelling, flexion and extension difficulties, and joint pain in the study group before treatment were (5.65±0.52) points, (5.44±0.16) points, and (5.35±0.12) points; the scores of joint swelling, flexion and extension difficulties, and joint pain in the study group after treatment were (1.83±0.15) points, (2.60±0.31) points, and (1.99±0.19) points. After treatment, the scores of joint swelling, flexion and extension difficulties, and joint pain in the control group were significantly lower than those before treatment (p<0.05), and the scores of joint swelling, flexion and extension difficulties, and joint pain in the research group were significantly lower than those before treatment (p<0.01). After treatment, the scores of joint swelling, flexion and extension difficulties, and joint pain in the research group were significantly lower than those in the control group (p<0.05), and the difference was statistically significant (the results are shown in Figure 1 shown).

[0042] 2..2 Comparison of knee joint function

[0043] The knee joint function of mice in each group was compared before and after treatment. The knee joint function score of the control group was (48.10±4.84) ​​points before treatment and (32.6±3.06) points after treatment. The knee joint function score of the study group was (48.70±3.94) points before treatment and (16.20±3.70) points after treatment. The knee joint function score of the control group was significantly lower than that before treatment (p<0.01), and the knee joint function score of the study group was significantly lower than that before treatment (p<0.0001). The knee joint function score of mice in the study group after treatment was significantly lower than that in the control group after treatment (p<0.05), and the difference was statistically significant (results as shown in Figure 2 shown).

[0044] Peripheral ankylosing spondylitis mainly manifests itself in pain and swelling of large joints, such as the hip and knee joints, which may cause joint deformity and movement disorders in severe cases. This experiment found that Chinese medicine fumigation treatment can significantly improve the TCM syndrome and knee joint function symptoms of mice with peripheral ankylosing spondylitis, indicating that this Chinese medicine fumigation can improve the inflammation of the joints in mice with peripheral ankylosing spondylitis.

[0045] The above contents are merely examples and explanations of the concept of the present invention. The technicians in this technical field may make various modifications or additions to the specific embodiments described or replace them in a similar manner. As long as they do not deviate from the concept of the invention or exceed the scope defined by the claims, they should all fall within the protection scope of the present invention.

Claims

1. A Chinese medicine fumigation and washing prescription for treating peripheral ankylosing spondylitis, characterized in that: The active ingredients are prepared from the following weights of Chinese medicinal raw materials: Processed Aconite 5-15g, White Peony 5-10g, Deer Antler 5-15g, Epimedium 5-15g, Salvia miltiorrhiza 5-10g, Panax notoginseng 5-15g, Stephania tetrandra 5-15g, Siegesbeckia 5-15g, Malus 5-15g, Zanthoxylum bungeanum 5-10g, Curcuma 5-15g, Notopterygium wilfordii 5-10g, Sophora flavescens 5-10g, Cuscuta australis 5-15g.

2. The Chinese medicine fumigation and washing prescription according to claim 1, characterized in that: The active ingredients are prepared from the following weights of Chinese medicinal raw materials: 10g of cooked aconite root, 8g of white peony root, 10g of deer antler, 10g of epimedium, 8g of salvia miltiorrhiza, 10g of Panax notoginseng, 10g of Stephania tetrandra, 10g of Siegesbeckia herb, 10g of Kunming crabapple, 6g of Zanthoxylum bungeanum, 10g of Curcuma zedoaria, 8g of Notopterygium wilfordii, 6g of Sophora flavescens, and 10g of Cuscuta australis.

3. A method for preparing the Chinese medicine fumigation and washing prescription as claimed in claim 1 or 2, characterized in that: Mix the above medicinal materials, add 3-5 times the weight of water, soak for 30 minutes, and then boil for 30 minutes; fumigate with water vapor when using.

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