Application of stable silicic acid aqueous solution in preparation of product for treating acne

Abstract

The invention relates to an application of a stable silicic acid aqueous solution in preparation of a product for treating acne, and the mass concentration of the stable silicic acid aqueous solution is 0.5-2%. The product taking the stable silicic acid aqueous solution as an active ingredient can be used for treating acne or preventing and / or treating sebaceous gland homeostasis imbalance, can fade the color of sebaceous gland spots at the mass concentration of 0.5-2%, and has an anti-acne effect, and meanwhile, the stable silicic acid aqueous solution is low in raw material cost, environment-friendly, safe and suitable for industrial production. The tretinoin can be used as a traditional tretinoin substitute medicine with a good curative effect.

Description

Technical Field

[0001] The invention relates to the technical field of biomedicine, and in particular to the application of a stable silicic acid aqueous solution in the preparation of a product for treating acne. Background Art

[0002] Acne is a chronic inflammatory skin disease that occurs in the hair follicle sebaceous gland unit. In the early stage, it is mostly acne and inflammatory papules. After a long time, inflammation will occur, and pustules, nodules, and even non-removable scars can be seen. The course of the disease is long, and repeated attacks may leave pigmentation or scars. It is prone to occur in areas with strong sebum secretion such as the face and chest and back, which has a serious impact on the patient's physiology, psychology, and social interaction, and may cause depression or even suicide. It is one of the most common disfiguring skin diseases in dermatology. Some studies have shown that excessive sebum secretion and changes in lipid composition are the core of the occurrence and development of acne, and run through the entire process of acne. Sebaceous glands are holocrine glands. Androgens can stimulate the proliferation and differentiation of new sebaceous gland cells, while synthesizing lipids and moving to the center of the gland to form small lipid droplets. When the content of lipid droplets in the cytoplasm reaches a certain level, the sebaceous gland cells will disintegrate and release lipids.

[0003] Currently, in the clinical treatment of acne, the drugs used to treat sebaceous gland homeostasis are mainly retinoic acid. Although they are effective in inhibiting sebum secretion, long-term systemic use of such drugs may cause liver damage, intestinal mucosal damage, cardiovascular disease, teratogenicity, mental and psychological problems and many other adverse reactions. Summary of the invention

[0004] The purpose of the present invention is to address the problem of drugs for sebaceous gland homeostasis imbalance and propose an alternative drug, which utilizes a stable silicic acid aqueous solution to prepare a product for treating acne to obtain better therapeutic effects.

[0005] To achieve the above object, the present invention provides an application of a stable silicic acid aqueous solution in the preparation of a product for treating acne, wherein the mass concentration of the stable silicic acid aqueous solution is 0.5% to 2%.

[0006] Furthermore, the present invention provides an application of a stable silicic acid aqueous solution in the preparation of a product for preventing and / or treating sebaceous gland homeostasis imbalance, wherein the mass concentration of the stable silicic acid aqueous solution is 0.5% to 2%.

[0007] Preferably, when used for external application, the stable silicic acid aqueous solution is dispersed in an ointment base as an active ingredient.

[0008] Furthermore, the present invention provides a product for treating acne or for preventing and / or treating sebaceous gland homeostasis imbalance, wherein the active ingredient of the product is a stable silicic acid aqueous solution with a mass concentration of 0.5% to 2%, and the product is a cosmetic, a medical device or a drug.

[0009] Based on the above technical solution, the advantages of the present invention are:

[0010] The stable silicic acid aqueous solution of the present invention is used as an active ingredient. At a mass concentration of 0.5% to 2%, it can reduce the area of ​​sebaceous gland spots of golden hamsters, lighten the color of sebaceous gland spots, and has an anti-acne effect. At the same time, the raw material cost of the stable silicic acid aqueous solution is low, and it is environmentally friendly and safe, and can be used as a traditional retinoic acid alternative drug with good therapeutic effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] The drawings described herein are used to provide a further understanding of the present invention and constitute a part of this application. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the drawings:

[0012] Figure 1 These are photos of the appearance of sebaceous gland spots of golden hamsters in each group;

[0013] Figure 2 The appearance and size of the sebaceous gland spots of the golden hamsters in each group;

[0014] Figure 3 The figure is the histopathological manifestation of sebaceous gland spots in each group after medication. DETAILED DESCRIPTION

[0015] The technical solution of the present invention is further described in detail below through the accompanying drawings and embodiments.

[0016] The invention provides an application of a stable silicic acid aqueous solution in preparing a product for treating acne, wherein the mass concentration of the stable silicic acid aqueous solution is 0.5% to 2%.

[0017] It should be noted that the stable silicic acid aqueous solution in the present invention is a stable stable silicic acid aqueous solution, wherein the stable silicic acid aqueous solution refers to a solution in a stable state without polymerization, and its main components include monosilicic acid (H4SiO4), metasilicic acid (H2SiO3) and hydrogen silicate (HSiO3 - ), the mass concentration of 0.5% to 2% in the present invention means that the total concentration of the main components is 0.5% to 2%.

[0018] In order to illustrate the application effect of the stable silicic acid aqueous solution in the preparation of the product for treating acne, this example takes the sebaceous gland spots of golden hamsters as an example to simulate human acne for illustration:

[0019] The golden hamster, also known as the Syrian hamster, has sebaceous gland spots on its back, which are mainly composed of hair follicles, sebaceous glands and melanin masses, and are androgen-dependent organs. The anatomical structure and physiological response to hormones are similar to those of humans, making it an ideal animal model for studying abnormal sebum metabolism in acne. Therefore, the golden hamster is often used as an animal model for skin pathology research, especially in the field of acne research. Sebaceous gland spots refer to small lumps formed when the sebaceous glands are overactive. They are particularly common on the flanks of golden hamsters and can be used to simulate the pathological process of human acne.

[0020] Experimental animals: 0.5%, 1%, 2% stable silicic acid aqueous solution as active ingredient, dispersed in the ointment matrix of commercial medical water-in-oil cream matrix, as the test drug for easy use; retinoic acid; 30 SPF-grade, male, 6-7 weeks old, weighing 110±10g golden hamsters. They were placed in SPF-grade animal rooms with a constant temperature of 22±2℃ and 12 hours per day and night, and were raised in cages and fed with special feed for golden hamsters.

[0021] Experimental grouping: 30 golden hamsters were adaptively raised for 10 days before the experiment. According to the random number method, the animals were divided into groups, 5 in each group, for a total of 6 groups. Blank group, matrix group, test drug (0.5%), test drug (1.0%), test drug (2.0%), positive control group (0.1% retinoic acid cream), each group was raised in two cages.

[0022] Modeling test index: Sebaceous gland spot area measurement. On the 0th day of medication, the size of the sebaceous gland spots on the back of the golden hamster was measured with a vernier caliper under natural light, expressed as the maximum horizontal diameter × the maximum vertical diameter, and the sebaceous gland spot area was calculated. The size of the sebaceous gland spot was measured every 3 days and photographed for archiving.

[0023] Pathological examination: HE staining of skin tissue was used to observe the pathological changes of sebaceous glands. On the 25th day of medication, the skin tissue of sebaceous glands on the back of the golden hamster was removed and fixed in 4% formaldehyde for 24 hours. After that, tissue dehydration, paraffin embedding, paraffin sectioning and HE staining were started to observe the thickness and area of ​​the sebaceous glands, the number of lobes, the number of overlapping layers of sebaceous glands and the looseness of the sebaceous glands. HE staining operation steps:

[0024] (1) Dehydration: Place the fixed skin tissue in a dehydration box and place it in a gradient of ethanol from low to high (75% ethanol, 90% ethanol, 90% ethanol, 100% ethanol I, 100% ethanol II, 100% ethanol III dehydration) for 1 hour each.

[0025] (2) Transparency: Xylene I, xylene II, and xylene III are transparent for 45 minutes each.

[0026] (3) Wax immersion: Paraffin I, Paraffin II, and Paraffin III for 1 hour each.

[0027] (4) Embedding: After the skin tissue is dipped in wax, place it vertically in an embedding frame and continue to slowly pour liquid paraffin into the frame. After the paraffin solidifies, take out the embedded wax block and make corrections and markings.

[0028] (5) Sectioning: First trim the excess wax on the surface of the wax block, and when the tissue is about to be exposed, place it in the microtome. After 5 μm sectioning, gently place the specimen in 42°C warm water to flatten it, pick it up with a slide and bake it at 60°C to dry.

[0029] (6) Dewaxing and hydration: Place the sections in a 60°C oven and dry for 1 hour. Immerse the sections in xylene I and xylene II for 15 minutes each, 100% alcohol, 95% alcohol, 90% alcohol, 80% alcohol, and 70% wine for 5 minutes each, and then place them in distilled water for 3 minutes.

[0030] (7) Staining: Hematoxylin staining solution for 5 minutes.

[0031] (8) Washing: Rinse with running water for about 15 minutes to make the slices turn blue (or put them in alkaline water), but be careful not to run too much water to prevent the slices from falling off.

[0032] (9) Differentiation: Place the slices in 1% hydrochloric acid ethanol solution for about 2 seconds to fade. When the slices turn red and then lighter in color, they are ready.

[0033] (10) Rinse: Place the slices in running water to restore their blue color.

[0034] (11) Dehydration I: Immerse the slices in 50% ethanol → 70% ethanol → 80% ethanol for 3-5 min each.

[0035] (12) Counterstaining: Use 0.5% eosin ethanol solution for counterstaining for 1 min.

[0036] (13) Dehydration II: Place the slices in 95% ethanol to wash away excess red, then place them in anhydrous ethanol for 3-5 minutes. Finally, use absorbent paper to absorb excess ethanol.

[0037] (14) Transparency: Place the slices in xylene I and II for 3-5 min each.

[0038] (15) Sealing: Seal with neutral gum.

[0039] This example uses golden hamsters to explore the effects of different concentrations of the test drug on the sebaceous glands, and the following results are obtained:

[0040] The effect of external application of different concentrations of the tested drugs on reducing the area of ​​sebaceous gland spots in golden hamsters and lightening the color of sebaceous gland spots

[0041] During the experiment, the living conditions of the golden hamsters in each group were normal, and there were no obvious adverse reactions in the whole body or local area. As the medication time prolonged, the sebaceous gland spots in the control group and the matrix group became darker and larger in area.

[0042] Compared with the control group and matrix group mice, the sebaceous gland spots of mice in the different concentrations of test drug groups and positive group mice all became lighter in color and shrank in area to varying degrees. However, there were no significant differences between the different concentrations of test drug groups, nor between the test drug groups and the positive control group. Figure 1 , Figure 2 shown.

[0043] The histopathological observation of the sebaceous glands of mice in different experimental groups was conducted. After HE staining, it was observed under the microscope that the sebaceous glands of the blank group were intact in structure, with a large number of sebaceous gland lobes, which were distributed in a lobed manner, large and full, with many overlapping layers, mostly 3 layers, and tightly arranged. Compared with the blank group, there was no obvious change in the sebaceous gland structure in the matrix group; compared with the blank group, the number of glands and the overlapping layers of gland lobes in the 0.5% test drug, 1.0 test drug, and 2.0 test drug groups were relatively reduced, and the glands were arranged relatively loosely, which was similar to the results of the positive drug group. Figure 3 As shown, it has the potential to be an alternative to traditional retinoic acid drugs with good therapeutic effects.

[0044] Through animal experiments, the effects of different concentrations of the test drugs on the mouse model of skin acne (acne, a chronic inflammatory skin disease of the hair follicle sebaceous gland unit) showed that the stable silicic acid aqueous solution as the active ingredient, with a mass concentration of 0.5% to 2%, can reduce the area of ​​sebaceous gland spots in golden hamsters, lighten the color of sebaceous gland spots, and has an anti-acne effect.

[0045] Since acne is essentially a manifestation of sebaceous gland homeostasis imbalance, the stable silicic acid aqueous solution of the present invention can also be used in the preparation of products for preventing and / or treating sebaceous gland homeostasis imbalance, wherein the mass concentration of the stable silicic acid aqueous solution is 0.5% to 2%.

[0046] Furthermore, the present invention provides a product for treating acne or for preventing and / or treating sebaceous gland homeostasis imbalance, wherein the active ingredient of the product is a stable silicic acid aqueous solution with a mass concentration of 0.5% to 2%, and the product is a cosmetic, a medical device or a drug.

[0047] The stable silicic acid aqueous solution of the present invention is used as an active ingredient. At a mass concentration of 0.5% to 2%, it can reduce the area of ​​sebaceous gland spots of golden hamsters, lighten the color of sebaceous gland spots, and has an anti-acne effect. At the same time, the raw material cost of the stable silicic acid aqueous solution is low, and it is environmentally friendly and safe, and can be used as a traditional retinoic acid alternative drug with good therapeutic effects.

[0048] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, ordinary technicians in the field should understand that the specific implementation methods of the present invention can still be modified or some technical features can be replaced by equivalents without departing from the spirit of the technical solution of the present invention, which should be included in the scope of the technical solution for protection of the present invention.

Claims

1. Application of a stable silicic acid aqueous solution in the preparation of a product for treating acne, wherein: The mass concentration of the stable silicic acid aqueous solution is 0.5% to 2%.

2. Use of a stable silicic acid aqueous solution in the preparation of a product for preventing and / or treating sebaceous gland homeostasis imbalance, wherein: The mass concentration of the stable silicic acid aqueous solution is 0.5% to 2%.

3. The use according to claim 1 or 2, characterized in that: When used for external application, the stable silicic acid aqueous solution is dispersed in an ointment base as an active ingredient.

4. A product for treating acne or for preventing and / or treating sebaceous gland homeostasis imbalance, characterized in that: The active ingredient of the product is a stable silicic acid aqueous solution with a mass concentration of 0.5% to 2%, and the product is a cosmetic, a medical device or a medicine.

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