Beauty method and beauty device

Abstract

The invention provides a beauty treatment method and a beauty treatment device, and belongs to the technical field of beauty treatment, the beauty treatment method comprises the following steps: applying a beauty treatment essence containing collagen to target skin; radio-frequency current and / or light waves emitted by the beauty instrument are / is adopted to irradiate the target skin coated with the beauty essence, so that the target skin at least absorbs part of the beauty essence. According to the beautifying method provided by the embodiment of the invention, the collagen can be supplemented to the target skin through the mutual synergistic effect of the beautifying essence containing the collagen and the radio-frequency current and / or light waves emitted by the radio-frequency beautifying instrument, repairing and reconstruction of the autologous collagen are promoted, and the beautifying effect on the target skin is remarkably improved.

Description

Technical Field

[0001] The present application belongs to the field of beauty technology, and in particular, relates to a beauty method and a beauty device. Background Art

[0002] Currently, when using beauty devices for skin beautification, the beauty devices are usually used in combination with gel to assist the beauty device in sliding on the skin surface and reduce the friction between the beauty device and the skin.

[0003] However, in the related art, the gel used in conjunction with the beauty instrument is mainly a hydrating and moisturizing ingredient. Although it can assist the beauty instrument in sliding on the skin surface and has a certain hydrating and moisturizing effect, it itself is of little help in improving the beauty effect. The beauty effect is still mainly dependent on the beauty instrument, which affects the beauty effect. Summary of the invention

[0004] The purpose of the present application is to provide a beauty method and a beauty device, aiming to solve the problem that the beauty effect of traditional beauty methods is mediocre and to further improve the beauty effect.

[0005] The first aspect of the embodiment of the present application provides a cosmetic method, comprising:

[0006] Apply beauty essence containing collagen to the target skin;

[0007] The radio frequency current and / or light waves emitted by the beauty device are irradiated on the target skin coated with the beauty essence, so that the target skin absorbs at least part of the beauty essence.

[0008] Optionally, the beauty instrument is configured to emit a first light wave with a wavelength of 630nm-760nm and a second light wave with a wavelength of 760nm-1940nm; and / or a radio frequency current with a frequency of 0.8MHz-3.5MHz.

[0009] Optionally, the radio frequency current and / or light waves emitted by the beauty device are irradiated on the target skin for 1 min-5 min.

[0010] Optionally, the collagen-containing beauty essence also includes bioactive peptides.

[0011] Optionally, the mass proportion of collagen in the beauty essence containing collagen is 5%-15%;

[0012] And / or, the mass proportion of bioactive peptides in the beauty essence containing collagen is 1%-5%.

[0013] Optionally, the bioactive peptide comprises palmitoyl tripeptide-1.

[0014] Optionally, the collagen includes recombinant type I collagen, recombinant type III collagen and recombinant type XVII collagen.

[0015] Optionally, the mass ratio of recombinant type I collagen to recombinant type III collagen is 1:(2-3);

[0016] And / or, the mass ratio of recombinant type I collagen to recombinant type XVII collagen is (2-3):1.

[0017] Optionally, the collagen-containing beauty essence also includes alpine edelweiss stem cells, tranexamic acid and plant soothing agents.

[0018] Optionally, the mass proportion of alpine edelweiss stem cells in the beauty essence containing collagen is 1%-3%;

[0019] And / or, the mass proportion of tranexamic acid in the beauty essence containing collagen is 1%-5%;

[0020] And / or, the mass proportion of the plant allergy-relieving agent in the beauty essence containing collagen is 0.0625%-0.5%.

[0021] Optionally, the alpine edelweiss stem cells include alpine edelweiss callus extract.

[0022] Optionally, the plant allergy-relief agents include Sophora flavescens root extract, Glycyrrhiza inflata root extract and Scutellaria baicalensis root extract.

[0023] A second aspect of the embodiments of the present application provides a beauty device for implementing the above beauty method.

[0024] Compared with the prior art, the embodiments of the present application have the following beneficial effects: in the above-mentioned beauty method, the beauty essence containing collagen is first applied to the target skin, and then the radio frequency current and / or light waves emitted by the beauty instrument are irradiated on the target skin coated with the beauty essence containing collagen, so that the target skin absorbs at least part of the beauty essence containing collagen. On the one hand, the beauty essence containing collagen helps to provide lubrication for the radio frequency beauty instrument and reduce the friction of the radio frequency beauty instrument on the target skin; on the other hand, the beauty essence containing collagen can form a protective film on the surface of the target skin, activate the regeneration of autologous collagen, and the beauty instrument and the collagen in the beauty essence containing collagen interact with each other, causing the collagen to fission. The fissioned collagen is more easily absorbed by the target skin, further promoting the repair and reconstruction of autologous collagen and improving the beauty effect. That is, the beauty method provided in the embodiment of the present application can supplement collagen to the target skin and promote the repair and reconstruction of autologous collagen through the synergistic effect between the beauty essence containing collagen and the radio frequency current and / or light waves emitted by the radio frequency beauty device, thereby significantly improving the beauty effect on the target skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 Schematic diagram of the beauty method provided in the embodiment of the present application

[0026] Figure 2 This is an analysis diagram of the effects of alpine edelweiss stem cells on the face provided in the examples of this application;

[0027] Figure 3 This is an analysis diagram of the effect of tranexamic acid on IL-37 levels in the examples of this application;

[0028] Figure 4 This is an analysis diagram of the soothing effect of plant allergy-relieving agents on SLS stimulation in the examples of this application;

[0029] Figure 5 This is an analysis diagram of the effect of the plant allergy-relieving agent on the IL-1α secretion caused by SLS stimulation in the examples of this application;

[0030] Figure 6 This is an analysis diagram of the effect of the plant allergy-relieving agent on the transepidermal water loss caused by SLS in the examples of this application;

[0031] Figure 7 This is a comparative analysis diagram of skin elasticity R2 values ​​with different treatments in the embodiments of the present application;

[0032] Figure 8 This is a comparative analysis chart of skin firming F4 values ​​of different treatments in the examples of this application;

[0033] Fig. 9 This is a comparative analysis diagram of the proliferation of fibroblasts treated differently in the examples of this application;

[0034] Fig.10 This is a comparative analysis diagram of skin thickness with different treatments in the embodiments of the present application;

[0035] Fig.11 This is a comparative analysis diagram of skin density with different treatments in the embodiments of this application;

[0036] Fig.12 This is a comparative analysis diagram of the chin angles treated differently in the embodiments of the present application;

[0037] Fig.13 This is a comparative analysis diagram of the improvement of skin wrinkle volume under different treatments in the embodiments of the present application;

[0038] Fig.14 This is a comparative analysis chart of the improvement of skin wrinkle areas under different treatments in the embodiments of the present application. DETAILED DESCRIPTION

[0039] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present application more clearly understood, the present application is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and are not used to limit the present application.

[0040] In the following, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features. Thus, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the embodiments of the present application, words such as "exemplary", "or", "for example" are used to indicate examples, illustrations or explanations. Any embodiment or design described as "exemplary" or "for example" in the embodiments of the present application should not be interpreted as being more preferred or more advantageous than other embodiments or designs. Specifically, the use of words such as "exemplary", "or", "for example" is intended to present related concepts in a concrete way.

[0041] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those generally understood by technicians in the technical field in this application. The terms used in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application. It should be understood that, unless otherwise specified in this application, " / " means or. For example, A / B can represent A or B. "And / or" in this application is only a description of the association relationship of associated objects, indicating that three relationships can exist. For example, A and / or B can represent: A exists alone, A and B exist at the same time, and B exists alone. "At least one" means one or more. "Multiple" means two or more than two. For example, at least one of a, b or c can represent: a, b, c, a and b, a and c, b and c, a, b and c seven situations.

[0042] The first aspect of the embodiment of the present application provides a cosmetic method, such as Figure 1 As shown, including:

[0043] Step 110, applying the beauty essence containing collagen to the target skin;

[0044] Step 120: irradiate the target skin coated with the beauty essence with radio frequency current and / or light waves emitted by the beauty instrument, so that the target skin absorbs at least part of the beauty essence.

[0045] In the beauty method provided in the embodiment of the present application, a beauty essence containing collagen is first applied to the target skin, and then the radio frequency current and / or light waves emitted by the beauty device are irradiated on the target skin coated with the beauty essence containing collagen, so that the target skin absorbs at least part of the beauty essence containing collagen.

[0046] On the one hand, the beauty essence containing collagen helps to provide lubrication for the beauty instrument and reduce the friction of the beauty instrument on the target skin; on the other hand, the beauty essence containing collagen can form a protective film on the surface of the target skin, activate the regeneration of autologous collagen, and the beauty instrument and the beauty essence containing collagen interact with each other to cause collagen fission. The fissioned collagen is more easily absorbed by the target skin, further promoting the repair and reconstruction of autologous collagen and improving the beauty effect. That is, the beauty method provided in the embodiment of the present application can supplement collagen to the target skin and promote the repair and reconstruction of autologous collagen through the synergistic effect of the beauty essence containing collagen and the radio frequency current and / or light waves emitted by the radio frequency beauty instrument, significantly improving the beauty effect on the target skin.

[0047] As we age, the production of adenosine triphosphate (ATP) in skin cells will decrease, resulting in insufficient energy supply to cells, which in turn affects the normal metabolism and function of cells. The decrease in adenosine triphosphate (ATP) will lead to a decrease in mitochondrial function, and mitochondria will produce more free radicals, which will further damage the cell structure and function. At the same time, the function of fibroblasts decreases, and some of them are converted into apoptotic cells and senescent cells. Fibroblasts are mainly responsible for the production of proteins such as collagen to maintain the elasticity and firmness of the skin, but as the function of cells decreases, the synthesis of proteins such as collagen decreases, causing the skin to lose elasticity and firmness. In addition, as we age, the activity of cell autophagy will also decrease, resulting in the inability to clear damaged organelles and proteins in cells in time, further leading to the accumulation of apoptotic cells and senescent cells, and accelerating skin aging. Senescent cells will also lead to an increase in the secretion of MMPs (matrix metalloproteinases), which can degrade the extracellular matrix (ECM). ECM includes elastic networks such as collagen, elastin, non-collagen glycoproteins, aminoglycans and proteoglycans. Therefore, the increase in MMPs leads to accelerated degradation of ECM, resulting in loss of skin elasticity and firmness.

[0048] The beauty method provided in the embodiment of the present application can repair the skin from multiple dimensions such as promoting the production of its own collagen, repairing the extracellular matrix (ECM), replenishing collagen, inhibiting MMPs, and reducing collagen loss through the synergistic effect of the beauty essence containing collagen and the radio frequency current and / or light waves emitted by the beauty instrument, thereby improving the beauty effect.

[0049] In some embodiments, the beauty instrument is configured to emit a first light wave with a wavelength of 630nm-760nm and a second light wave with a wavelength of 760nm-1940nm; and / or a radio frequency current with a frequency of 0.8MHz-3.5MHz.

[0050] Among them, the first light wave with a wavelength of 630nm-760nm helps to repair the regenerated extracellular matrix components through cell action, stabilize the extracellular matrix structure, reduce the flow of extracellular matrix, and promote the fission of new collagen in skin cells. The second light wave with a wavelength of 760nm-1940nm can penetrate the dermis of the skin, heat the skin from shallow to deep, generate an endogenous heat field, and promote the growth of its own collagen matrix. Radio frequency current with a frequency of 0.8MHz-3.5MHz can generate heat in the skin tissue. This heat is mainly concentrated in the dermis, because the frequency and characteristics of the radio frequency current enable it to penetrate the epidermis and penetrate into the dermis. The collagen in the dermis shrinks after being heated, making the skin tighter. In addition, radio frequency current can also stimulate the regeneration of autologous collagen.

[0051] That is, the beauty instrument provided in the embodiment of the present application can have both biological and photothermal effects by simultaneously emitting a first radio frequency wave with a wavelength of 630nm-760nm and a second radio frequency wave with a wavelength of 760nm-1940nm, and has multiple effects such as collagen fission, collagen promotion and collagen repair, which promotes the reorganization of the skin collagen network and improves the beauty effect on the skin. The beauty instrument provided in the embodiment of the present application can also generate heat stimulation to the skin by emitting a radio frequency current with a frequency of 0.8MHz-3.5MHz, promote the regeneration of autologous collagen, and increase the elasticity and thickness of the skin. The beauty instrument can also coordinate the beauty essence containing collagen through radio frequency current and / or light waves, so that the collagen in the beauty essence fissions, enhances the absorption of collagen, further promotes the reorganization of the skin collagen network, and improves the beauty effect.

[0052] In some embodiments, the radio frequency current and / or light waves emitted by the beauty device are irradiated on the target skin for 1 min to 5 min. By setting the irradiation time to 1 min to 5 min, it can be ensured that the radio frequency current and / or the generated heat energy and / or light energy can stimulate the target skin, promote the production of its own collagen and skin firmness, and promote the fission of collagen in the beauty essence, improve the skin's absorption of collagen in the beauty essence, and can effectively reduce local thermal damage, pigmentation, skin stiffness, and thinning of the skin stratum corneum caused by radio frequency current and / or long-term irradiation on a target skin.

[0053] Exemplarily, the duration for which the radio frequency current generated by the beauty device irradiates the target skin may be 1 min, 2 min, 3 min, 4 min or 5 min.

[0054] In some embodiments, the collagen-containing beauty serum further comprises bioactive peptides.

[0055] That is to say, beauty essences containing collagen include both collagen and bioactive peptides. Through the synergistic effect of the two, the synthesis of substances such as collagen and glycosaminoglycans in the extracellular matrix (ECM) can be increased, the dermis of the skin can be strengthened, and the skin can be plump and elastic.

[0056] Specifically, bioactive peptides have a smaller molecular weight and can more easily penetrate the skin barrier and enter the deep layers of the skin. Bioactive peptides that enter the deep layers of the skin can further promote the absorption and utilization of collagen, allowing collagen to play a better role. At the same time, the presence of collagen can also provide a good carrier for bioactive peptides, which helps to stably and persistently release bioactive peptides in the skin. Bioactive peptides have the function of regulating skin cell functions, can stimulate the proliferation and differentiation of skin cells, and promote skin repair and regeneration. Collagen can enhance the elasticity and firmness of the skin and improve problems such as skin sagging and wrinkles. Therefore, the interaction between collagen and bioactive peptides can further regulate skin functions.

[0057] In some embodiments, the collagen accounts for 5%-15% by weight of the cosmetic essence containing collagen.

[0058] By setting the mass proportion of collagen in beauty essence containing collagen to 5%-15%, it helps collagen enter the dermis of the skin under the action of radio frequency current and / or light waves emitted by the beauty instrument, improve sagging skin, and assist cells in generating new collagen, which can reduce skin irritation and allergic reactions caused by excessive collagen proportion. In addition, excessive collagen proportion cannot be absorbed by the skin, which easily leads to waste of effective ingredients.

[0059] In some embodiments, the bioactive peptide accounts for 1%-5% by weight of the cosmetic essence containing collagen.

[0060] The mass proportion of bioactive peptides in beauty essences containing collagen is set within the range of 1%-5%, which can ensure that they can fully exert their skin care effects, produce synergistic effects with the collagen in the beauty essence containing collagen, and avoid problems such as skin irritation or allergies caused by excessive concentrations.

[0061] In some embodiments, the bioactive peptide comprises palmitoyl tripeptide-1.

[0062] Palmitoyl tripeptide-1 can directly act on the dermis of the skin, promote the synthesis of extracellular matrix (such as collagen, glycosaminoglycans), strengthen the dermis, make the skin thicker and firmer, effectively prevent and reduce wrinkles, and have a stronger ability to resist ultraviolet radiation.

[0063] Palmitoyl tripeptide-1 is a peptide component that can penetrate the skin barrier and send signals to the skin, stimulating the proliferation of fibroblasts and thereby enhancing the synthesis of collagen. As a signaling molecule, palmitoyl tripeptide-1 can act precisely on skin cells to avoid excessive stimulation of the skin. Collagen, as a natural ingredient, has good biocompatibility and safety. The combination of the two can reduce skin discomfort and allergic reactions and improve the overall safety of skin care products.

[0064] In order to verify the relevant effects of palmitoyl tripeptide-1, the inventors conducted anti-wrinkle tests using palmitoyl tripeptide-1.

[0065] Among them, in the anti-wrinkle test, 23 volunteers aged 39 to 74 were recruited and divided into a control group and an experimental group. The experimental group applied a cream containing 3% palmitoyl tripeptide-1 to their faces every day, and the control group used water as a placebo instead of 3% palmitoyl tripeptide-1. After 2 months, the anti-wrinkle effect was evaluated by two-step contour measurement and photography technology. The results are shown in Table 1:

[0066] Table 1 Comparison of anti-wrinkle effects of different skin treatments

[0067]

[0068] In Table 1, negative values ​​indicate a decrease, positive values ​​indicate an increase, * indicates p<0.05 in a significant analysis, and ** indicates p<0.01 in a significant analysis. As shown in Table 1, the experimental group has a significant anti-wrinkle effect compared to the control group.

[0069] In some embodiments, the collagen includes recombinant type I collagen, recombinant type III collagen, and recombinant type XVII collagen.

[0070] Among them, recombinant type I collagen is a relatively hard collagen that is distributed in the bones and tendon tissues of the human body. It can play a role in shaping and supporting, enhancing the elasticity and firmness of the skin, and promoting the regeneration and repair of skin cells. Recombinant type III collagen can form a protective film on the surface of the skin, stimulate the regeneration of autologous collagen, and also promote the reconstruction of skin collagen, strengthen its own barrier, and inhibit the production of inflammatory factors. Recombinant type XVII collagen can inhibit the activity of MMPs (matrix metalloproteinases) and reduce the loss of collagen.

[0071] In some embodiments, the mass ratio of recombinant type I collagen to recombinant type III collagen is 1:(2-3).

[0072] The inventors have conducted in-depth research on the changes in the morphology and proportion of type I collagen and type III collagen in the skin of people of different ages. From the fetal stage to the elderly stage, the total amount of collagen decreases with age, and the content of type I collagen and type III collagen in the skin also shows a trend of continuous decrease. Among them, compared with the infant group, the decrease in type I collagen in the skin of the elderly group was 20.8%, and the decrease in type III collagen was 74.45%. In other words, the decrease in type III collagen is greater, and the ratio of type I collagen and type III collagen content in aging skin continues to increase. Therefore, in order to supplement type I collagen and type III collagen, and restore the ratio between the two, the embodiment of the present application sets the mass ratio of recombinant type I collagen and recombinant type III collagen to 1: (2-3).

[0073] In some embodiments, the mass ratio of recombinant type I collagen to recombinant type XVII collagen is (2-3):1.

[0074] Recombinant type I collagen mainly plays a role in supporting and maintaining elasticity, while recombinant type XVII collagen is a transmembrane protein that plays an important role in maintaining the health of the basement membrane and anti-aging of cells. By controlling the mass ratio of the two within the above range, the synergistic effect can be fully exerted, the advantages of each can be brought into play, and the effect can be balanced.

[0075] In some embodiments, the beauty essence containing collagen further includes alpine edelweiss stem cells, tranexamic acid and a plant soothing agent.

[0076] Alpine edelweiss stem cells can inhibit the activity of elastase in the skin and promote the production of endothelial protein, which can slow down the degradation of elastin and maintain skin elasticity. They also have a certain inhibitory effect on bacteria and have antibacterial and anti-inflammatory effects.

[0077] Tranexamic acid can inhibit ultraviolet-induced melanin deposition, improve and inhibit skin inflammation, repair the skin barrier, and effectively whiten and lighten spots.

[0078] Plant allergy-relieving agents can inhibit the synthesis of inflammatory factors in the skin, reduce vascular permeability, relieve inflammatory symptoms such as heat, redness, swelling, pain, and itching, and have a mild and long-lasting effect.

[0079] In some embodiments, the Leontopodium alpinum stem cells include Leontopodium alpinum callus extract.

[0080] The extract of alpine edelweiss callus contains rich antioxidant substances, which can neutralize free radicals and reduce the damage of oxidative stress to the skin. Through the antioxidant effect, it can protect skin cells from damage and delay the aging process of the skin. The active ingredients in the extract of alpine edelweiss callus can also stimulate the regeneration and repair process of skin cells and promote the healing of damaged skin tissue. In addition, the extract of alpine edelweiss callus also has certain antibacterial and anti-inflammatory effects, promotes the disappearance of inflammation, and helps to improve skin health.

[0081] Exemplarily, in order to improve the activity of alpine edelweiss stem cells and ensure their stability, other auxiliary agents such as glycerol, xanthan gum and citric acid may also be added.

[0082] In some embodiments, the botanical allergy-relief agents include Sophora flavescens root extract, Glycyrrhiza inflata root extract, and Scutellaria baicalensis root extract.

[0083] Sophora flavescens, Licorice and Scutellaria baicalensis all have rich anti-inflammatory plant active ingredients. Among them, Sophora flavescens root extract can relieve skin stress and discomfort, Licorice inflata root extract can reduce skin burning irritation, and Scutellaria baicalensis root extract has active ingredients such as baicalin and baicalein, which have significant anti-inflammatory, antioxidant and bacterial growth inhibition effects. Through the synergistic effect of the three, better biological efficacy can be exerted, the synthesis of inflammatory factors can be effectively inhibited, and vascular permeability can be reduced. The plant-derived ingredients are mild and long-lasting.

[0084] For example, in order to ensure the activity and stability of the plant allergy-relieving agent, other auxiliary agents such as water and butylene glycol may be added.

[0085] In some embodiments, the mass proportion of alpine edelweiss stem cells in the beauty essence containing collagen is 1%-3%.

[0086] The inventors tested 34 female subjects using a cream containing 2% by mass of alpine edelweiss stem cells. The average age of the subjects was 59 years old (ranging from 41 to 71 years old). The subjects were randomly divided into two groups. The experimental group applied a cream containing 2% by mass of alpine edelweiss stem cells to the face twice a day, while the control group replaced the alpine edelweiss stem cells with an equal amount of placebo and also applied it to the face twice a day.

[0087] like Figure 2 As shown in the figure, after six weeks of experiment, the tear grooves of the experimental group were significantly lightened and the cheek contours were significantly improved. However, there was no significant change in the control group.

[0088] It should be noted that in the above experiment, the alpine edelweiss stem cells were made from alpine edelweiss callus extract.

[0089] In some embodiments, the mass proportion of tranexamic acid in the beauty essence containing collagen is 1%-5%.

[0090] In order to investigate the effect of tranexamic acid on inhibiting skin inflammation, the inventors conducted relevant experiments. The experimental method was as follows: 30 patients with erythematotelangiectatic rosacea were selected and divided into two groups on average. One group was used as the treatment group and 3% tranexamic acid was applied to the affected area. The other group was used as the control group and distilled water was applied twice a day. After two weeks, the level of IL-37 factor was measured by dot hybridization method.

[0091] like Figure 3 As shown, treatment with 3% tranexamic acid significantly reduced the level of IL-37 and had a good effect in inhibiting inflammation.

[0092] In some embodiments, the mass proportion of the plant soothing agent in the beauty essence containing collagen is 0.0625%-0.5%.

[0093] The inventors studied the effects of plant allergy-relieving agents on reducing cell damage, lowering inflammatory responses and restoring the skin barrier.

[0094] Experiment 1:

[0095] In vitro experiments were conducted using a 3D Episkin model. The 3D Episkin model was treated with 1.25% SLS (sodium lauryl sulfate) and different concentrations of plant allergens for 42 hours. The cell viability in different treatments was then detected using the MTT colorimetric method. The results are as follows: Figure 4 As shown:

[0096] like Figure 4 As shown, when the mass concentration of the plant allergy-relieving agent is in the range of 0.0625%-0.5%, it can cause irritation to SLS. Among them, when the concentration of the plant allergy-relieving agent is 0.5%, its soothing effect can reach 54.3%.

[0097] Experiment 2:

[0098] In vitro experiments were conducted using a 3D Episkin model. The 3D Episkin model was treated with 1.25% SLS (sodium lauryl sulfate) and different concentrations of plant allergy-relieving agents for 42 hours. The concentration of IL-1α in different treatments was then detected by ELISA. The results are as follows: Figure 5 As shown:

[0099] like Figure 5 As shown, when the mass concentration of the plant allergy-relieving agent is in the range of 0.0625%-0.5%, it can inhibit the IL-1α secretion caused by SLS.

[0100] Experiment 3:

[0101] The in vivo experiment was conducted on the inner forearm skin of 14 subjects. The inner forearm skin was washed before the test, and a closure patch containing 5% SLS and different concentrations was applied. The patch was removed after 24 hours of closure, and the TM300 transepidermal water loss tester was used to test the transepidermal water loss TMWT of different treatments, and the difference value was calculated. Figure 6 As shown:

[0102] like Figure 6 As shown, plant soothing agents can effectively reduce the transepidermal water loss caused by SLS and help restore the skin barrier.

[0103] The second aspect of the embodiment of the present application provides a beauty device for implementing the above beauty method. The beauty device provided by the embodiment of the present application has all the beneficial effects of the beauty method described above, which will not be repeated here.

[0104] The following examples are further described in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present application and are not intended to limit the scope of the present application. The experimental methods in the following examples that do not specify specific conditions are usually based on the conditions recommended by the manufacturer.

[0105] 33 Asian adult female subjects aged 30 to 40 were recruited to use the beauty method provided in the embodiments of the present application for 7 consecutive days, 14 days and 28 days, and the changes in parameters such as the subjects' skin elasticity R2 value, skin firmness F4 value, skin thickness, skin density, chin angle, skin wrinkles, and fibroblasts were measured.

[0106] Among them, the recruited subjects met the following conditions: 1. Healthy Asian adult women between the ages of 30-40; 2. Having facial looseness, sagging, and lack of elasticity; 3. Visible lines in the brow area; 4. Visible lines in the forehead area; 5. The level of crow's feet in the corner of the eye area is 3 or above (according to the QLSY assessment atlas); 6. The level of forehead wrinkles in the forehead area is 3 or above; 7. The level of nasolabial folds in the nose area is 3 or above; 8. The skin in the evaluation area has no obvious skin damage, scars, hair, etc.

[0107] The ingredients of the beauty essence containing collagen are: water, bifid yeast fermentation product filtrate, butylene glycol, 1,3-propylene glycol, glyceryl polyether-26, erythritol, glycerin, PEG-240 / HDI copolymer bis-decyltetradecyl polyether-20 ether, 1,2-hexanediol, p-hydroxyacetophenone, PEG / PPG-14 / 7 dimethyl ether, tranexamic acid, carbomer, arginine, diphenylsiloxy phenyl trimethicone, disodium EDTA, triethylhexanoin, polyglyceryl-10 Myristate, PPG-26-buteth-26, collagen, palmitolerin, sodium hyaluronate, ethylhexylglycerin, PEG-40 hydrogenated castor oil, (daily use) fragrance, scutellaria root extract, glycyrrhiza uralensis root extract, sophora flavescens root extract, β-glucan, palmitoyl tripeptide-1, methylpropanediol, leontopodium alpinum callus extract, potassium laurate, tocopherol (vitamin E), caprylhydroxamic acid, citric acid, xanthan gum; the radio frequency beauty instrument chooses EG50 Golden Jasmine Collagen Ultimate Beauty Instrument.

[0108] In the beauty essence containing collagen, the content of collagen is 10%, the content of palmitoyl tripeptide-1 is 3%, the content of alpine edelweiss stem cells is 2%, the content of tranexamic acid is 3%, and the content of plant allergen is 0.25% (all the above are mass ratios). The contents of the remaining components are formulated according to the requirements of conventional beauty essences in the field and are not specifically described here.

[0109] During the test, 33 subjects were divided into an experimental group and a control group. In the experimental group, the beauty essence containing collagen was first applied to the corresponding skin, and then the skin after the application of the beauty essence containing collagen was treated with a beauty instrument. The treatment time for each part was 2 minutes. In the control group, the beauty essence containing collagen was replaced with a commercially available ordinary moisturizing gel, which did not contain collagen, palmitoyl tripeptide-1, alpine edelweiss stem cells, tranexamic acid and plant allergy-relieving agent ingredients.

[0110] The beauty instrument can emit a first light wave with a wavelength of 630nm-760nm, a second light wave with a wavelength of 760nm-1940nm, and a radio frequency current with a frequency of 0.8MHz-3.5MHz during operation. It should be noted that the beauty instrument used the same working mode in all tests of the experimental group and the control group.

[0111] The test methods for each indicator are as follows:

[0112] Skin elasticity R2 value and skin firmness F4 value: the evaluation site is one side of the subject's cheek, the evaluation instrument is a skin elasticity tester (model: Cutometer dual MPA580), and the evaluation time is before the test, after 14 days of use, and after 28 days of use;

[0113] Skin thickness and skin density: The evaluation site is one side of the subject's cheek, the evaluation instrument is a skin ultrasound device (U1trasound), and the evaluation time is before the test, after 14 days of use, and after 28 days of use;

[0114] Fibroblasts: The evaluation site was one side of the subject's cheek, the evaluation instrument was a live cell analyzer (model: Incucyte SX5), and the evaluation time was before the test, 7 days after use, 14 days after use, and 28 days after use;

[0115] Chin angle: The evaluation site is the front face of the subject, the evaluation instrument is VISIA-CR + Inage Pro Plus image analysis software, and the evaluation time is before the test, immediately after the test, 14 days after use, and 28 days after use;

[0116] Skin wrinkle volume: The evaluation sites include the subject's forehead wrinkles, one side of the nasolabial folds, one side of the crow's feet, one side of the under-eye wrinkles and one side of the marionette lines. The evaluation instrument is the Canfieldsci skin rapid optical imaging system PRIMOS-lite. The evaluation time is before the test, after 14 days of use, and after 28 days of use.

[0117] Skin wrinkle area: The evaluation areas include the subject's forehead wrinkles, one side of nasolabial folds, one side of glabellar wrinkles, one side of crow's feet, one side of under-eye wrinkles and one side of marionette wrinkles. The evaluation instrument is Canfieldsci's skin rapid optical imaging system PRIMOS-lite. The evaluation time is before the test, after 14 days of use, and after 28 days of use.

[0118] like Figure 7 As shown, in the experimental group, compared with before use, after 14 days of use, the average value of the R2 value of the skin elasticity of the subjects increased by 14.82%, and after 28 days of use, the average value of the R2 value of the skin elasticity of the subjects increased by 18.08%, both of which were significantly different from before use. In the control group, compared with before use, after 14 days of use, the average value of the R2 value of the skin elasticity of the subjects increased by 6.74%, and after 28 days of use, the average value of the R2 value of the skin elasticity of the subjects increased by 7.74%. Therefore, before use, there was no significant difference in the R2 value of skin elasticity of the subjects in the experimental group and the control group, but after 14 and 28 days of use, the average value of the R2 value of skin elasticity of the subjects in the experimental group increased more, which was significantly different from the control group.

[0119] like Figure 8As shown, in the experimental group, compared with before use, the average skin firmness F4 value of the subjects decreased by 17.34% after 14 days of use, and decreased by 17.82% after 28 days of use, both of which were significantly different from before use. In the control group, compared with before use, the average skin firmness F4 value of the subjects decreased by 5.86% after 14 days of use, and decreased by 7.35% after 28 days of use. Therefore, before use, there was no significant difference in the skin firmness F4 value of the subjects in the experimental group and the control group, but after 14 and 28 days of use, the average skin firmness F4 value of the subjects in the experimental group increased more significantly, which was significantly different from the control group.

[0120] like Fig. 9 As shown, in the experimental group, compared with before use, 7 days after use, the average value of fibroblast (collagen) proliferation of the subjects increased by 158.32%, after 14 days of use, the average value of fibroblast (collagen) proliferation of the subjects increased by 161.82%, and after 28 days of use, the average value of fibroblast (collagen) proliferation of the subjects increased by 166.73%. In the control group, compared with before use, 7 days after use, the average value of fibroblast (collagen) proliferation of the subjects increased by 36.6%, after 14 days of use, the average value of fibroblast (collagen) proliferation of the subjects increased by 40.86%, and after 28 days of use, the average value of fibroblast (collagen) proliferation of the subjects increased by 44.23%. Therefore, before use, there was no significant difference in the average value of fibroblast (collagen) proliferation of the subjects in the experimental group and the control group, but after 7 days, 14 days and 28 days of use, the average value of fibroblast (collagen) proliferation of the subjects in the experimental group increased more, which was significantly different from the control group.

[0121] like Fig.10 As shown, compared with before use, after 14 days of use, the average skin thickness of the subjects increased by 7.42%, and after 28 days of use, the average skin thickness of the subjects increased by 9.36%, which was a significant difference compared with before use.

[0122] like Fig.11 As shown, compared with before use, after 14 days of use, the average skin density of the subjects increased by 11.67%, and after 28 days of use, the average skin density of the subjects increased by 13.16%, which was a significant difference compared with before use.

[0123] like Fig.12As shown, compared with before use, immediately after use (i.e., tested immediately after use), the average chin angle of the subjects decreased by 1.09%, after 14 days of use, the average chin angle of the subjects decreased by 2.80%, and after 28 days of use, the average chin angle of the subjects decreased by 2.92%, which was a significant difference compared with before use.

[0124] like Fig.13 As shown in the results, compared with before use, after 14 days and 28 days of use, the wrinkle volume of the subjects' forehead wrinkles, one side of the nasolabial folds, one side of the crow's feet, one side of the under-eye wrinkles and one side of the marionette lines were reduced. Fig.13 As shown in (A), after 14 days of use, the average volume of the subjects' forehead wrinkles decreased by 24.30%. Fig.13 As shown in (B), after 14 days of use, the average volume of the subjects' nasolabial folds decreased by 12.16%. Fig.13 As shown in (C), after 14 days of use, the average volume of the subjects' crow's feet wrinkles decreased by 17.91%. Fig.13 As shown in (D), after 14 days of use, the average volume of the subjects' under-eye wrinkles decreased by 17.75%. Fig.13 As shown in (E), after 14 days of use, the average volume of the subjects' marionette lines decreased by 14.45%;

[0125] like Fig.13 As shown in (A), after 28 days of use, the average volume of the subjects' forehead wrinkles decreased by 25.03%, which was a significant difference compared to before use. Fig.13 As shown in (B), after 28 days of use, the average volume of the subjects' nasolabial folds decreased by 13.82%. Fig.13 As shown in (C), after 28 days of use, the average volume of the subjects' crow's feet wrinkles decreased by 19.88%. Fig.13 As shown in (D), after 28 days of use, the average volume of the wrinkles under the eyes of the subjects decreased by 21.78%. Fig.13 As shown in (E), after 28 days of use, the average volume of the subjects' marionette lines decreased by 18.77%.

[0126] like Fig.14 As shown, compared with before use, after 14 days and 28 days of use, the skin wrinkle areas of the subjects' forehead wrinkles, one side of nasolabial folds, one side of glabellar wrinkles, one side of crow's feet, one side of under-eye wrinkles and one side of marionette lines were reduced.

[0127] Among them, Fig.14 As shown in (A), after 14 days of use, the average area of ​​the subjects' forehead wrinkles decreased by 20.70%. Fig.14As shown in (B), after 14 days of use, the average area of ​​the subjects' nasolabial folds decreased by 8.16%. Fig.14 As shown in (C), after 14 days of use, the average area of ​​the subjects' glabellar lines decreased by 16.61%. Fig.14 As shown in (D), after 14 days of use, the average area of ​​the subjects' crow's feet wrinkles decreased by 18.64%. Fig.14 As shown in (E), after 14 days of use, the average area of ​​the subjects' under-eye wrinkles decreased by 22.44%. Fig.14 As shown in (F), after 14 days of use, the average area of ​​the subjects' marionette lines decreased by 19.38%.

[0128] like Fig.14 As shown in (A), after 28 days of use, the average area of ​​the subjects' forehead wrinkles decreased by 22.80%. Fig.14 As shown in (B), after 28 days of use, the average area of ​​the subjects' nasolabial folds decreased by 9.23%, which was a significant difference compared to before use. Fig.14 As shown in (C), after 28 days of use, the average area of ​​the subjects' glabellar lines decreased by 17.39%. Fig.14 As shown in (D), after 28 days of use, the average area of ​​the subjects' crow's feet wrinkles decreased by 20.31%. Fig.14 As shown in (E), after 28 days of use, the average area of ​​the subjects' under-eye wrinkles decreased by 24.64%. Fig.14 As shown in (F), after 28 days of use, the average area of ​​the subjects' marionette lines decreased by 21.31%;

[0129] In summary, by using the beauty method provided in the embodiment of the present application to treat the skin, the elasticity, firmness, skin thickness, skin density, chin angle, skin wrinkles and fibroblasts (collagen level) of the skin can be significantly improved, and the beauty effect can be improved from multiple dimensions. This is mainly due to the beauty method provided in the embodiment of the present application, through the synergistic effect of the beauty essence containing collagen and the radio frequency beauty instrument, the beauty effect produced on the skin from multiple aspects such as photothermal effect and biological effect, relative to the technical solution of using beauty essence alone or radio frequency beauty instrument alone with ordinary gel in the prior art, unexpected technical effects are produced. In addition, by comparing the test results of skin elasticity, skin firmness and fibroblast (collagen) proliferation of the experimental group and the control group, it can be seen that, relative to the method of using moisturizing gel with a beauty instrument in the prior art, the embodiment of the present application can significantly improve the improvement of skin elasticity, skin firmness and fibroblast (collagen) proliferation by using the beauty instrument containing collagen and the beauty instrument, and has a better beauty effect.

[0130] The embodiments described above are only used to illustrate the technical solutions of the present application, rather than to limit them. Although the present application has been described in detail with reference to the aforementioned embodiments, a person skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. Such modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present application, and should all be included in the protection scope of the present application.

Claims

1. A cosmetic method, characterized in that: include: Apply beauty essence containing collagen to the target skin; The radio frequency current and / or light waves emitted by the beauty instrument are irradiated on the target skin coated with the beauty essence containing collagen, so that the target skin absorbs at least a part of the beauty essence containing collagen.

2. The cosmetic method according to claim 1, characterized in that: The beauty instrument is configured to emit a first light wave with a wavelength of 630nm-760nm and a second light wave with a wavelength of 760nm-1940nm; and / or a radio frequency current with a frequency of 0.8MHz-3.5MHz.

3. The cosmetic method according to claim 1, characterized in that: The radio frequency current and / or the light wave emitted by the beauty device irradiates the target skin for a time period of 1 min to 5 min.

4. The cosmetic method according to claim 1, characterized in that: The collagen-containing beauty essence also includes bioactive peptides.

5. The cosmetic method according to claim 4, characterized in that: The mass proportion of the collagen in the beauty essence containing collagen is 5%-15%; And / or, the bioactive peptide accounts for 1%-5% by weight in the beauty essence containing collagen.

6. The cosmetic method according to claim 4, characterized in that: The bioactive peptides include palmitoyl tripeptide-1.

7. The cosmetic method according to any one of claims 1 to 6, characterized in that: The collagen includes recombinant type I collagen, recombinant type III collagen and recombinant type XVII collagen.

8. The cosmetic method according to claim 7, characterized in that: The mass ratio of the recombinant type I collagen to the recombinant type III collagen is 1:(2-3); And / or, the mass ratio of the recombinant type I collagen to the recombinant type XVII collagen is (2-3):

1.

9. The cosmetic method according to claim 4, characterized in that: The beauty essence containing collagen also includes alpine edelweiss stem cells, tranexamic acid and plant allergy-relieving agents.

10. The cosmetic method according to claim 9, characterized in that: The mass proportion of the alpine edelweiss stem cells in the collagen-containing beauty essence is 1%-3%; And / or, the mass proportion of the tranexamic acid in the beauty essence containing collagen is 1%-5%; And / or, the mass proportion of the plant soothing agent in the beauty essence containing collagen is 0.0625%-0.5%.

11. The cosmetic method according to claim 9, characterized in that: The alpine edelweiss stem cells include alpine edelweiss callus extract.

12. The cosmetic method according to claim 9, characterized in that: The plant allergy-relieving agent includes a root extract of Sophora flavescens, a root extract of Glycyrrhiza inflata and a root extract of Scutellaria baicalensis.

13. A beauty device, characterized in that: Used to implement the cosmetic method according to any one of claims 1 to 12.

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