Selenium-rich oligopeptide bio-organic fertilizer and preparation method thereof

Abstract

The invention provides a selenium-rich oligopeptide bio-organic fertilizer and a preparation method thereof, and the preparation method comprises the following steps: S1, preparing an organic fertilizer matrix, and carrying out mixed fermentation on livestock and poultry manure and plant straw; s2, when the fermentation temperature of the livestock and poultry manure and the plant straw begins to drop and tends to 30-40 DEG C, uniformly mixing the fermentation mixture with lactobacillus and inorganic selenium salt, and performing strip pile fermentation; s3, when the fermentation temperature begins to rise and exceeds 40 DEG C, quickly turning the pile, and adding a microbial agent, inorganic salt and a trace element additive in the pile turning process; and S4, when the fermentation temperature begins to drop again, uniformly mixing the fermentation mixture with oligopeptide and humic acid, and after the inorganic selenium salt completely reacts, completing fermentation to obtain the selenium-rich oligopeptide bio-organic fertilizer. The selenium content in the organic fertilizer can be increased, and plant absorption is facilitated.

Description

Technical Field

[0001] The invention relates to the technical field of bio-organic fertilizers, and in particular to a selenium-enriched oligopeptide bio-organic fertilizer and a preparation method thereof. Background Art

[0002] Selenium is an essential trace element for the human body. It has the functions of anti-oxidation, anti-aging, protecting and repairing cells, improving human immunity, removing heavy metal poisoning, and preventing cancer. It is known as the "fire of life", "king of anti-cancer" and "longevity element". With the rise of the development boom of many selenium-rich agricultural products, selenium-rich agriculture has gradually come into people's view as a high value-added product. Fruits, vegetables and tea are daily necessities. They have high development and utilization prospects for supplementing human selenium through selenium-rich fruits and vegetables. Develop the production of selenium-rich fruits, vegetables and tea, increase the added value of fruits, vegetables and tea, and improve people's quality of life. Different crop varieties have different selenium collection capabilities, and leafy vegetables basically have no selenium collection capabilities. Due to the uneven distribution of selenium in the soil and the lack of regeneration ability, the commercial development value of producing selenium-rich vegetables based on natural selenium-rich soil is not great. Only a few areas in China have rich selenium content in the soil, and selenium-rich products can be produced. But the quantity is limited, and it is difficult to meet the current demand for selenium-rich products. At present, most of the selenium-enriched products on the market are produced by foliar spraying. However, due to the low technical content and the untimely absorption of crops, not only the selenium supplementation effect is poor, but also the selenium element flows into the environment, causing environmental pollution of soil and water. When selenium products are directly applied to the soil in the form of fertilizers, the effectiveness of selenium supplementation is low due to the soil's fixation of selenate. Increasing the amount of selenium fertilizer will also cause environmental pollution and the effect is unstable. Summary of the invention

[0003] In view of the deficiencies in the prior art, the present invention provides a selenium-enriched oligopeptide bio-organic fertilizer and a preparation method thereof, which improves the absorption and utilization rate of selenium by crops, thereby increasing crop yield and selenium content.

[0004] According to an embodiment of the present invention, a method for preparing selenium-enriched oligopeptide bio-organic fertilizer comprises the following steps:

[0005] S1, preparing organic fertilizer matrix by mixing livestock and poultry manure with plant straw for fermentation;

[0006] S2, when the fermentation temperature of livestock and poultry manure and plant straw begins to drop and tends to 30°C to 40°C, the fermentation mixture is evenly mixed with lactobacillus and inorganic selenium salt, and then subjected to strip-pile fermentation;

[0007] S3, when the fermentation temperature starts to rise and exceeds 40°C, the compost is quickly turned, and microbial agents, inorganic salts, and trace element additives are added during the turning process;

[0008] S4, when the fermentation temperature begins to drop again, the fermentation mixture is evenly mixed with oligopeptides and humic acid, and the fermentation is completed after the inorganic selenium salt reacts completely to obtain selenium-rich oligopeptide bio-organic fertilizer.

[0009] Preferably, in S1, the mass ratio of livestock and poultry manure to plant straw is 6-8:1-2, and the fermentation time is 10-50 days.

[0010] Further preferably, in step S2, the amount of lactobacillus added is 0.25-0.5% of the mass of the fermentation mixture, and the inorganic selenium salt is sodium selenite or the amount of inorganic selenium salt added is 0.05-0.1% of the mass of the fermentation mixture.

[0011] Further preferably, in step S3, the microbial agent includes one or more of Bacillus subtilis agent, such as gold agent and RW enzyme agent, and the inoculation amount of the microbial agent is 1×10 8 ---1×10 9 CFU / g.

[0012] Further preferably, in step S3, the inorganic salt is one or more of inorganic salts containing potassium ions, ammonium ions, and phosphate ions.

[0013] Further preferably, in step S3, the trace element additive is one or more water-soluble inorganic salts containing elements such as magnesium, calcium, and iron.

[0014] Further preferably, in step S4, the amount of oligopeptide added is 0.3-0.5% of the mass of the organic fertilizer matrix.

[0015] Further preferably, in step S4, the added amount of humic acid is 1-5% of the mass of the organic fertilizer matrix.

[0016] Still more preferably, the overall fermentation time of S2, S3 and S4 is 6 to 10 days.

[0017] According to an embodiment of the present invention, a selenium-enriched oligopeptide bio-organic fertilizer is also provided, which is prepared by the above-mentioned preparation method.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] When preparing the organic fertilizer matrix, a good fermentation environment can be formed in the initial stage of the fermentation of the organic fertilizer matrix. When the condition tends to the high activity interval of lactobacillus, the inorganic selenium salt, lactobacillus and the organic fertilizer matrix fermentation mixture are evenly mixed, so that the lactobacillus can be in a high activity state for a long time, which can not only ensure the promotion of the fermentation of the organic fertilizer matrix, but also quickly and efficiently carry out the enrichment and conversion of organic selenium. Moreover, when the temperature condition in the fermentation stage is high, the fermentation temperature can be well controlled by quickly turning the pile and mixing in microbial agents, inorganic salts and trace element additives, so as to ensure the activity of lactobacillus. Meanwhile, the inorganic salts and trace elements can improve the nutrient elements of the organic fertilizer, are conducive to the growth and metabolism of lactobacillus, and promote the conversion and enrichment of the inorganic selenium salt. DETAILED DESCRIPTION

[0020] The technical solution of the present invention is further described below in conjunction with embodiments.

[0021] Embodiment 1:

[0022] To prepare the organic fertilizer matrix, 80 kg of livestock and poultry manure and 10 kg of plant straw were mixed and fermented;

[0023] When the fermentation temperature of livestock and poultry manure and plant straw begins to drop and approaches 30°C, the fermentation mixture is evenly mixed with 0.018kg of lactobacillus and 0.00225kg of sodium selenite, and then subjected to strip-pile fermentation;

[0024] When the temperature inside the fermentation mixture begins to rise and exceeds 40°C, the compost is quickly turned over, and 1×10 8 CFU / g Bacillus subtilis agent, 0.05kg potassium phosphate, 0.04kg ammonium phosphate, 0.03kg magnesium chloride, 0.03kg calcium chloride, 0.03kg zinc chloride, mix well;

[0025] When the fermentation temperature begins to drop again, the fermentation mixture is evenly mixed with 0.27 kg of oligopeptides and 0.9 kg of humic acid. After the inorganic selenium salt reacts completely, the fermentation is completed to obtain selenium-rich oligopeptide bio-organic fertilizer A.

[0026] Embodiment 2:

[0027] To prepare the organic fertilizer matrix, 67.5 kg of livestock and poultry manure and 22.5 kg of plant straw were mixed and fermented;

[0028] When the fermentation temperature of livestock and poultry manure and plant straw begins to drop and approaches 30°C, the fermentation mixture is evenly mixed with 0.054kg of lactobacillus and 0.0045kg of sodium selenite, and then subjected to strip-pile fermentation;

[0029] When the temperature inside the fermentation mixture begins to rise and exceeds 40°C, the compost is quickly turned over, and 1×10 9 CFU / g Bacillus subtilis agent, 0.05kg potassium phosphate, 0.04kg ammonium phosphate, 0.03kg magnesium chloride, 0.03kg calcium chloride, 0.03kg zinc chloride, mix well;

[0030] When the fermentation temperature begins to drop again, the fermentation mixture is evenly mixed with 0.45 kg of oligopeptides and 4.5 kg of humic acid. After the inorganic selenium salt reacts completely, the fermentation is completed to obtain selenium-rich oligopeptide bio-organic fertilizer B.

[0031] Embodiment three:

[0032] To prepare the organic fertilizer matrix, 70 kg of livestock and poultry manure and 20 kg of plant straw were mixed and fermented;

[0033] When the fermentation temperature of livestock and poultry manure and plant straw begins to drop and approaches 30°C, the fermentation mixture is evenly mixed with 0.045kg of lactobacillus and 0.0036kg of sodium selenite, and then subjected to strip fermentation;

[0034] When the temperature inside the fermentation mixture begins to rise and exceeds 40°C, the compost is quickly turned over, and 1×10 9 CFU / g Bacillus subtilis agent, 0.05kg potassium phosphate, 0.04kg ammonium phosphate, 0.03kg magnesium chloride, 0.03kg calcium chloride, 0.03kg zinc chloride, mix well;

[0035] When the fermentation temperature begins to drop again, the fermentation mixture is evenly mixed with 0.36 kg of oligopeptides and 2.7 kg of humic acid, and the fermentation is completed after the inorganic selenium salt reacts completely to obtain selenium-rich oligopeptide bio-organic fertilizer C.

[0036] The present invention also provides multiple groups of comparative examples, including:

[0037] Comparative Example 1: 80 kg of livestock and poultry manure, 10 kg of plant straw, 0.018 kg of lactobacillus, 0.00225 kg of sodium selenite, 1×10 8 CFU / g Bacillus subtilis agent, 0.05kg potassium phosphate, 0.04kg ammonium phosphate, 0.03kg magnesium chloride, 0.03kg calcium chloride, 0.03kg zinc chloride, 0.27kg oligopeptide and 0.9kg humic acid were mixed evenly and fermented in strip piles to obtain organic fertilizer D.

[0038] Comparative Example 2: To prepare an organic fertilizer matrix, 80 kg of livestock and poultry manure and 10 kg of plant straw were mixed and fermented;

[0039] When the fermentation temperature of livestock and poultry manure and plant straw begins to drop and approaches 30°C, the fermentation mixture is evenly mixed with 0.018kg of lactobacillus and 0.00225kg of sodium selenite, and then subjected to strip-pile fermentation;

[0040] When the temperature inside the fermentation mixture begins to rise and exceeds 40°C, the compost is quickly turned over, and 1×10 8 CFU / g Bacillus subtilis agent was mixed evenly;

[0041] When the fermentation temperature begins to drop again, the fermentation mixture is evenly mixed with 0.27 kg of oligopeptides and 0.9 kg of humic acid. After the inorganic selenium salt reacts completely, the fermentation is completed to obtain the selenium-rich oligopeptide bio-organic fertilizer E.

[0042] Comparative Example 3: Preparation of organic fertilizer matrix: 80 kg of livestock and poultry manure, 10 kg of plant straw, 0.27 kg of oligopeptide, 0.9 kg of humic acid, 1×10 8 CFU / g Bacillus subtilis agent is mixed evenly and fermented into ordinary organic fertilizer F.

[0043] The organic fertilizers prepared in Examples 1-3 and Comparative Examples 1-3 were subjected to a fertilization experiment for comparison. The application method was: fertilization was carried out in different fields in the same area, and hybrid rice was planted, 10,000 rice plants were planted per mu of land, and 120 kg of fertilizer was used per mu of land. The plant height and stem girth of the rice were measured at the same time, and after the rice matured, the grains in each area were taken to measure the selenium content. (Experimental comparison data table 1):

[0044] Table 1

[0045]

[0046] It can be seen from the above measurement results that the organic fertilizer prepared by the present invention can effectively increase the selenium content in grains; compared with the method of mixing and fermenting all the raw materials together, adding corresponding raw materials at different times according to the fermentation conditions can effectively increase the selenium content in the organic fertilizer, which is convenient for plant absorption; and after adding corresponding inorganic salts and trace elements, it can promote the growth and metabolism of lactobacillus, improve the activity of lactobacillus, thereby improving the ability of lactobacillus to enrich and convert organic selenium during the fermentation process. After the fermentation is completed, slightly excessive inorganic salts and trace elements can also be used as inorganic fertilizers for plant growth, which is beneficial to plant growth and development.

[0047] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the purpose and scope of the technical solution of the present invention, which should be included in the scope of the claims of the present invention.

Claims

1. A method for preparing selenium-enriched oligopeptide bio-organic fertilizer, characterized in that: The following steps are involved: S1, preparing organic fertilizer matrix by mixing livestock and poultry manure with plant straw for fermentation; S2, when the fermentation temperature of livestock and poultry manure and plant straw begins to drop and tends to 30°C to 40°C, the fermentation mixture is evenly mixed with lactobacillus and inorganic selenium salt, and then subjected to strip-pile fermentation; S3, when the fermentation temperature starts to rise and exceeds 40°C, the compost is quickly turned, and microbial agents, inorganic salts, and trace element additives are added during the turning process; S4, when the fermentation temperature begins to drop again, the fermentation mixture is evenly mixed with oligopeptides and humic acid, and the fermentation is completed after the inorganic selenium salt reacts completely to obtain selenium-rich oligopeptide bio-organic fertilizer.

2. The method for preparing a selenium-rich oligopeptide bio-organic fertilizer according to claim 1, characterized in that: In S1, the mass ratio of livestock and poultry manure to plant straw is 6-8:1-2, and the fermentation time is 10-50 days.

3. The method for preparing a selenium-rich oligopeptide bio-organic fertilizer according to claim 1, characterized in that: In step S2, the amount of lactobacillus added is 0.02-0.06% of the mass of the fermentation mixture, and the inorganic selenium salt is sodium selenite, and the amount of inorganic selenium salt added is 0.0025-0.005% of the mass of the fermentation mixture.

4. The method for preparing a selenium-rich oligopeptide bio-organic fertilizer according to claim 1, characterized in that: In step S3, the microbial agent includes one or more of Bacillus subtilis agent, Rujin agent and RW enzyme agent, and the inoculation amount of the microbial agent is 1×10 8 -1×10 9 CFU / g.

5. The method for preparing a selenium-rich oligopeptide bio-organic fertilizer according to claim 1, characterized in that: In step S3, the inorganic salt is one or more of inorganic salts containing potassium ions, ammonium ions, and phosphate ions.

6. The method for preparing a selenium-rich oligopeptide bio-organic fertilizer according to claim 1, characterized in that: In step S3, the trace element additive is one or more water-soluble inorganic salts containing elements such as magnesium, calcium, and zinc.

7. The method for preparing a selenium-rich oligopeptide bio-organic fertilizer according to claim 1, characterized in that: In step S4, the amount of oligopeptide added is 0.3-0.5% of the mass of the organic fertilizer matrix.

8. The method for preparing a selenium-enriched oligopeptide bio-organic fertilizer according to claim 6, characterized in that: In step S4, the added amount of humic acid is 1-5% of the mass of the organic fertilizer matrix.

9. A method for preparing a selenium-enriched oligopeptide bio-organic fertilizer according to any one of claims 1 to 8, characterized in that: The overall fermentation time of S2, S3 and S4 is 6 to 10 days.

10. A selenium-rich oligopeptide bio-organic fertilizer obtained by the preparation method according to claim 9.

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