Extraction equipment for phycoerythrin 545 extracting solution based on ultrasonication method

Abstract

The invention discloses extraction equipment for a phycoerythrin 545 extracting solution based on an ultrasonication method, and relates to the technical field of phycoerythrin extraction.The extraction equipment comprises a soundproof box, an ultrasonic generation module is arranged at the top of the soundproof box, an amplitude-change pole is arranged at the output end of the bottom of the ultrasonic generation module, and a limiting groove is formed in the inner side of the soundproof box; a limiting plate is slidably connected into the limiting groove, a supporting plate is arranged at the end of the limiting plate, a cooling cylinder is arranged above the supporting plate, a sample container is detachably connected to the middle of the cooling cylinder, and a lifting assembly used for driving the supporting plate to ascend and descend is arranged on the bottom side of the interior of the soundproof box. According to the sample crushing device, uniform distribution of samples in the sample container is achieved, the crushing effect is improved, the cooling effect on a sample solution is effectively guaranteed in the using process, and in addition, the cleaning effect on an amplitude-change pole is improved after the sample crushing device is used.

Description

Technical Field

[0001] The invention relates to the technical field of phycoerythrin extraction, in particular to an extraction device for phycoerythrin 545 extract based on an ultrasonic crushing method. Background Art

[0002] Phycoerythrin is a fluorescent phycobiliprotein separated and purified from red algae. As a fluorescent protein extracted from red algae, phycoerythrin has broad application prospects in biomedical research and clinical diagnosis. The extraction process of phycoerythrin usually includes raw material preparation, cell disruption, preliminary extraction, purification and other steps. The cell disruption process is used to release phycoerythrin from red algae cells, among which ultrasonic disruption is widely used in the cell disruption process.

[0003] Chinese patent application number 2017114829447 discloses an intelligent ultrasonic crusher, including a shell, a sealed door, a crushing chamber, and an ultrasonic probe assembly. The sealed door is provided with an observation window and a control module, and the control module includes a touch screen, a microcomputer, a wireless communication module, a timing module, and a storage module that are electrically connected to each other; a lifting device is provided in the center of the top of the crushing chamber, and an ultrasonic probe assembly and a photoelectric sensor are connected below the lifting device; a condensing device and a temperature sensor are provided at the bottom of the crushing chamber directly below the ultrasonic probe assembly, and three slide grooves are radially provided around the condensing device and the temperature sensor, and each slide groove is provided with connected vertical baffles and push-pull assemblies above and below, respectively, and the three slide grooves are at an angle of 120° to each other. However, during use, the sample in the sample container will be unevenly distributed, which will reduce the effect of cell crushing.

[0004] In addition, during the cell disruption process, ice cubes are usually used to cool the sample solution in an ice bath. However, during the cooling process, the ice cubes close to the sample container melt as the temperature of the sample solution rises, which will cause gaps between the ice cubes and the sample container. The ice cubes cannot directly contact the sample solution for heat exchange, thereby reducing the cooling effect on the sample solution. In addition, after use, the amplitude transformer needs to be manually cleaned, which is prone to blind spots in cleaning and leads to incomplete cleaning. Summary of the invention

[0005] The technical problem to be solved by the present invention is to overcome the existing defects and provide an extraction device for phycoerythrin 545 extract based on ultrasonic crushing method, which can evenly distribute the sample in the sample container, improve the crushing effect, and effectively ensure the cooling effect of the sample solution during use. In addition, after use, the amplitude transformer is thoroughly cleaned, which can effectively solve the problems in the background technology.

[0006] To achieve the above object, the present invention provides the following technical solution: an extraction device for phycoerythrin 545 extract based on ultrasonic crushing method, comprising a soundproof box, an ultrasonic generating module is provided on the top of the soundproof box, and a horn is provided at the bottom output end of the ultrasonic generating module; A limiting groove is provided on the inner side of the soundproof box, a limiting plate is slidably connected in the limiting groove, a supporting plate is provided at the end of the limiting plate, a cooling cylinder is provided above the supporting plate, and a sample container is detachably connected to the middle of the cooling cylinder; The bottom inner side of the soundproof box is provided with a lifting assembly for driving the support plate to lift and lower, the top of the support plate is provided with a rotating assembly for driving the cooling cylinder to rotate, and the inner side of the soundproof box is provided with a cleaning assembly for cleaning the amplitude transformer; The cleaning assembly includes a slide rail, the interior of the slide rail is rotatably connected to a bidirectional screw, both sides of the bidirectional screw are threadedly sleeved with support arms, the ends of the support arms on both sides are provided with fixed rods on the opposite sides, the fixed rods on both sides are provided with arc seats on the opposite ends, and the inner side of the arc seat is provided with an arc cleaning part.

[0007] Preferably, a sound insulation layer is provided inside the sound insulation box, a sealed door is movably connected to the front side of the sound insulation box, a sound insulation glass is embedded in the middle of the sealed door, the annular space between the sample container and the cooling cylinder forms a cooling groove, and a temperature sensor is provided on the inner top of the sound insulation box.

[0008] Preferably, the lifting assembly includes a guide rail, a stud is rotatably connected inside the guide rail, a sliding seat is threadedly sleeved on the stud, a movable rod is movably connected to the top of the sliding seat, the top of the movable rod is movably connected to the bottom of the support plate, a lifting motor is provided at the end of the guide rail, and the output shaft of the lifting motor is fixedly connected to the end of the stud.

[0009] Preferably, the guide rail is arranged on the inner bottom surface of the sound insulation box, and the sliding seat is slidably connected to the guide rail.

[0010] Preferably, the rotating assembly comprises a rotating seat and a rotating motor, a gear ring is fixedly sleeved on the circumferential surface of the rotating seat, and a rotating gear is meshed on the side surface of the gear ring.

[0011] Preferably, the rotating seat is rotatably connected to the top of the support plate, the top of the rotating seat is fixedly connected to the bottom of the cooling cylinder, the rotating motor is arranged on the limiting plate, and the output shaft of the rotating motor is fixedly connected to the rotating gear.

[0012] Preferably, the slide rail is slidably connected to the inner side of the sound insulation box, threads with opposite spiral directions are symmetrically opened on both sides of the bidirectional screw, the support arm is slidably connected to the slide rail, an electric telescopic rod is provided on the inner top side of the sound insulation box, the telescopic end of the electric telescopic rod is fixedly connected to the top of the slide rail, a drive motor is provided at the end of the slide rail, and the output shaft of the drive motor is fixedly connected to the end of the bidirectional screw.

[0013] Preferably, the centers of the arc-shaped cleaning portion and the arc-shaped seat coincide with the central axis of the amplitude changing rod, the arc-shaped cleaning portion and the arc-shaped seat are bonded by Velcro, stirring rods are provided at the bottom of the arc-shaped seats on both sides, and a paddle is provided at an inclined bottom end of the stirring rod on one side.

[0014] Compared with the prior art, the present invention has the following advantages: 1. After the crushing is completed, the support plate is located at the bottom, the arc-shaped cotton pad is soaked with alcohol, and fixed to the inner side of the arc-shaped seat by Velcro, and then the driving motor is used to drive the bidirectional screw to rotate to make the support arms on both sides move in opposite directions until the arc-shaped cotton pads on both sides completely wrap the circumference of the amplitude transformer, and then the electric telescopic rod is used to move the arc-shaped cleaning part from top to bottom to achieve wiping and cleaning of the amplitude transformer.

[0015] 2. After placing the sample container and ice cubes respectively, the height of the arc seat is adjusted to a position lower than the amplitude rod, and then the arc seats on both sides are moved toward each other until the stirring rod corresponds to the sample container. The bottom of the stirring rod is immersed in the sample solution by extending the electric telescopic rod, and the rotating component is used to drive the cooling cylinder to rotate and the drive motor to reciprocate slightly, so that the stirring rod can stir the sample solution evenly, and at the same time, the temperature of the middle and outer parts of the sample solution can be kept uniform.

[0016] 3. In the process of crushing red algae cells, when the temperature of the sample solution increases rapidly, the stirring rod is adjusted to correspond to the position of the cooling tank, and then the stirring rod is moved downward to extend into the ice cube. In this way, when the cooling cylinder is driven to rotate by the rotating assembly, the stirring rod on one side stirs the ice cube, and the stirring rod on the other side drives the paddle to pry the ice cube to one side of the sample container, thereby increasing the fit and stacking height of the ice cube and the sample container, increasing the contact area between the ice cube and the sample container, and improving the cooling efficiency of the sample solution. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the structure of the present invention; Figure 2 It is a schematic diagram of the internal structure of the present invention; Figure 3 It is a schematic diagram of the cross-sectional structure of the present invention; Figure 4 For the present invention Figure 3 The enlarged structural diagram at A in the middle; Figure 5 It is a front view structural schematic diagram of the present invention.

[0018] In the figure: 1. soundproof box; 101. sealing door; 102. ultrasonic generating module; 103. amplitude transformer; 104. limiting groove; 105. limiting plate; 106. supporting plate; 107. cooling cylinder; 108. sample container; 109. temperature sensing probe; 2. lifting assembly; 201. guide rail; 202. sliding seat; 203. stud; 204. lifting motor; 205. movable rod; 3. rotating assembly; 301. rotating seat; 302. gear ring; 303. rotating motor; 304. rotating gear; 4. cleaning assembly; 401. sliding rail; 402. bidirectional screw; 403. supporting arm; 404. fixing rod; 405. arc seat; 406. arc cleaning part; 407. stirring rod; 408. electric telescopic rod; 409. dial plate; 410. driving motor. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] See also Figure 1-5 The present embodiment provides an extraction device for phycoerythrin 545 extract based on ultrasonic crushing method, including a soundproof box 1, a soundproof layer is provided inside the soundproof box 1, a sealed door 101 is movably connected to the front side of the soundproof box 1, a soundproof glass is embedded in the middle of the sealed door 101, an ultrasonic generating module 102 is provided on the top of the soundproof box 1, and a horn 103 is provided at the bottom output end of the ultrasonic generating module 102. The ultrasonic generating module 102 includes an ultrasonic generator and a transducer. The ultrasonic generator generates high-frequency electrical signals, which are transmitted to the transducer through a cable. The transducer converts the high-frequency electrical signals generated by the ultrasonic generator into mechanical vibrations, and effectively transmits the vibrations generated by the transducer to the liquid through the horn 103. The soundproof box 1 also includes an external control terminal electrically connected to the ultrasonic generating module 102, which is used to adjust various parameters during cell crushing.

[0021] A limiting groove 104 is provided on the inner side of the soundproof box 1, and a limiting plate 105 is slidably connected in the limiting groove 104. A support plate 106 is provided at the end of the limiting plate 105. The cooperation between the limiting plate 105 and the limiting groove 104 is used to achieve the limitation of the support plate 106. A cooling cylinder 107 is provided above the support plate 106. A sample container 108 is detachably connected to the middle part of the cooling cylinder 107. The bottom of the cooling cylinder 107 clamps and fixes the sample container 108 through a clamping structure. The clamping structure is horizontally arranged and is preferably a clamping mechanism for cylinders commonly used in the prior art. A cooling groove is formed in the annular space between the sample container 108 and the cooling cylinder 107. The cooling groove is used to hold ice cubes to cool the sample container 108 in an ice bath. A temperature probe 109 is provided on the top of the inner side of the soundproof box 1. The temperature probe 109 is used to detect the temperature of the sample solution during the crushing process.

[0022] A lifting assembly 2 for driving the support plate 106 to rise and fall is provided on the inner bottom side of the soundproof box 1. The lifting assembly 2 includes a guide rail 201, which is provided on the inner bottom surface of the soundproof box 1. A stud 203 is rotatably connected inside the guide rail 201. A sliding seat 202 is threadedly sleeved on the stud 203. The sliding seat 202 is slidably connected to the guide rail 201. A movable rod 205 is movably connected to the top of the sliding seat 202. The top of the movable rod 205 is movably connected to the bottom of the support plate 106. A lifting motor 204 is provided at the end of the guide rail 201. The output shaft of the lifting motor 204 is fixedly connected to the end of the stud 203. The lifting motor 204 is used to drive the stud 203 to rotate. The limiting effect of the guide rail 201 is used to make the stud 203 drive the sliding seat 202 to move linearly, so that the movable rod 205 in the inclined state gradually approaches the vertical state. At the same time, the movable rod 205 pushes against the support plate 106 to move upward, thereby realizing the lifting and lowering of the cooling cylinder 107 and the sample container 108.

[0023] A cleaning assembly 4 for cleaning the horn 103 is provided on the inner side of the soundproof box 1. The cleaning assembly 4 includes a slide rail 401, which is slidably connected to the inner side of the soundproof box 1. A bidirectional screw 402 is rotatably connected inside the slide rail 401. The two sides of the bidirectional screw 402 are symmetrically provided with threads with opposite spiral directions. Support arms 403 are threadedly sleeved on both sides of the bidirectional screw 402. The support arms 403 are slidably connected to the slide rail 401. Fixed rods 404 are provided on the opposite sides of the ends of the support arms 403 on both sides. The fixed rods on both sides An arc-shaped seat 405 is provided at the opposite end of 404, and an arc-shaped cleaning portion 406 is provided on the inner side of the arc-shaped seat 405. A driving motor 410 is provided at the end of the slide rail 401, and the output shaft of the driving motor 410 is fixedly connected to the end of the bidirectional screw 402. The driving motor 410 is used to drive the bidirectional screw 402 to rotate, and the threads in opposite directions on both sides are used to make the support arms 403 on both sides move in opposite directions, thereby realizing the opposite movement of the arc-shaped seats 405 and the arc-shaped cleaning portion 406 on both sides, so that the arc-shaped cleaning portion 406 is close to the amplitude changing rod 103.

[0024] The arc-shaped cleaning portion 406 is preferably an arc-shaped cotton pad soaked in alcohol. The centers of the arc-shaped cleaning portion 406 and the arc-shaped seat 405 coincide with the central axis of the amplitude transformer 103, and the radius of the arc-shaped cleaning portion 406 on both sides is the same as that of the amplitude transformer 103, so that when the arc-shaped cleaning portion 406 is close to the amplitude transformer 103, the circumferential side surface of the amplitude transformer 103 is completely wrapped. The arc-shaped cleaning portion 406 and the arc-shaped seat 405 are bonded by Velcro to facilitate the disassembly and replacement of the arc-shaped cleaning portion 406.

[0025] An electric telescopic rod 408 is provided on the inner top side of the soundproof box 1. The telescopic end of the electric telescopic rod 408 is fixedly connected to the top of the slide rail 401. The telescopic movement of the electric telescopic rod 408 drives the slide rail 401 to rise and fall to adjust the height of the arc-shaped cleaning portion 406.

[0026] When in use, open the sealed door 101, place the sample container 108 in the middle of the cooling cylinder 107, and use the clamping structure to clamp and fix the container, place ice cubes in the cooling tank, and the ice cubes directly contact the outer wall of the sample container 108, start the lifting motor 204 to drive the stud 203 to rotate, so that the sliding seat 202 slides to the side away from the sealed door 101, and the inclined movable rod 205 gradually approaches the vertical state, and drives the support plate 106 to drive the cooling cylinder 107 and the sample container 108 to move upward until the bottom end of the amplitude rod 103 is immersed in the middle of the sample solution by a specified length, and then place the temperature sensing probe 109 in the sample container 108, close the sealed door 101, and then adjust various crushing parameters through the external control terminal, and then start the crushing program through the external control terminal, and the amplitude rod 103 starts to vibrate.

[0027] After the crushing is completed, the sealing door 101 is opened, the sample container 108 and the ice cubes are taken out in turn, and the support plate 106 is dropped to the bottom. The arc-shaped cotton pad soaked in alcohol is adhered to the inner side of the arc-shaped seat 405 by Velcro, and the drive motor 410 is started. The rotation of the bidirectional screw 402 drives the support arms 403 on both sides to move in opposite directions, so that the arc-shaped cleaning parts 406 on both sides are wrapped around the circumference of the amplitude rod 103, and then the electric telescopic rod 408 is started to extend, so that the arc-shaped cleaning part 406 moves from high to low along the amplitude rod 103, completing one wiping of the amplitude rod 103, and the number of wiping is preferably once to prevent the cleaned contaminants from re-attaching to the amplitude rod 103.

[0028] However, in actual use, the sample in the sample container 108 is unevenly distributed, resulting in uneven contact area between the ultrasonic wave and the cells during crushing, and the cell wall and cell membrane of the red algae cells cannot be effectively and thoroughly crushed, which reduces the crushing efficiency. In addition, it takes time for the ice cubes and the sample solution to exchange heat, resulting in a slow drop in the temperature of the middle part of the sample solution. Therefore, the following improvements are made: A rotating assembly 3 for driving the cooling cylinder 107 to rotate is provided on the top of the support plate 106. The rotating assembly 3 includes a rotating seat 301 and a rotating motor 303. The rotating seat 301 is rotatably connected to the top of the support plate 106. The top of the rotating seat 301 is fixedly connected to the bottom of the cooling cylinder 107. A gear ring 302 is fixedly sleeved on the circumferential surface of the rotating seat 301. A rotating gear 304 is meshed on the side of the gear ring 302. The rotating motor 303 is provided on the limiting plate 105. The output shaft of the rotating motor 303 is fixedly connected to the rotating gear 304. The rotating gear 304 is driven to rotate by the rotation of the rotating motor 303. The rotating gear 304 drives the rotating seat 301 to rotate by meshing with the gear ring 302, thereby realizing the rotation of the cooling cylinder 107 and the sample container 108.

[0029] Stirring rods 407 are provided at the bottom of the arc-shaped seats 405 on both sides.

[0030] When in use, place the sample container 108 containing the sample solution in the middle of the cooling cylinder 107 and place ice cubes in the cooling tank. At this time, the support plate 106 is at the bottom. First, use the electric telescopic rod 408 to drive the slide rail 401 to move downward so that the height of the arc seat 405 is lower than the bottom end of the amplitude rod 103. Then start the driving motor 410 to make the arc seats 405 on both sides move in opposite directions so that the stirring rods 407 on both sides correspond to the sample container 108 at the bottom. At this time, the arc seats 405 on both sides are not in contact with each other. Continue to use the extension of the electric telescopic rod 408 to immerse the bottom of the stirring rod 407 in the sample solution. Then start the rotating motor 303 and use the gear ring 302 to engage with the rotating gear 304 to drive The rotating seat 301 and the cooling cylinder 107 rotate to achieve relative movement between the stirring rod 407 and the sample solution, and at the same time, the driving motor 410 is rotated back and forth with a small amplitude, so that the stirring rods 407 on both sides are reciprocated with a small amplitude along the radial direction of the cooling cylinder 107, so as to achieve stirring of the sample solution, making the sample solution more uniform, and at the same time, achieving rapid heat exchange between the middle and the outside of the sample solution, so that the temperature of the sample solution drops rapidly and the temperature distribution of the sample solution is more uniform. Therefore, before cell disruption, the present invention achieves stirring of the sample solution by utilizing the coordination of the structure between the rotating component 3 and the cleaning component 4, thereby ensuring the uniformity of the sample solution to improve the disruption effect, and causing the temperature of the middle of the sample solution to drop rapidly.

[0031] In addition, during the crushing process, the temperature of the sample solution will gradually increase, and the ice cubes close to the outer wall of the sample container 108 will melt first. As the crushing time becomes longer and longer, the melting range of the ice cubes increases, resulting in the ice cubes being unable to fit the outer wall of the sample container 108. In addition, as the ice cubes melt, the height will also decrease, which will greatly reduce the cooling efficiency and cause the sample solution temperature to rise faster. Therefore, the following improvements are made: A paddle plate 409 is tilted at the bottom end of the stirring rod 407 on one side. The tilted arrangement of the paddle plate 409 enables the paddle plate 409 to paddle the ice cubes toward the sample container 108 when the paddle plate 409 and the cooling cylinder 107 rotate relative to each other.

[0032] In the process of crushing the red algae cells, when the temperature sensing probe 109 detects that the temperature of the sample solution is higher than the set threshold, it is actively judged that the temperature rises faster, the cooling efficiency of the ice cubes decreases, and the crushing requirements cannot be met. At this time, the driving motor 410 is started to make the support arms 403 on both sides move in opposite directions until the stirring rod 407 corresponds to the position of the cooling tank. At this time, the arc seats 405 on both sides are not closed, and the distance between the arc seats 405 and the amplitude rod 103 does not affect the normal operation of the amplitude rod 103. Then, the electric telescopic rod 408 is used to drive the slide rail 401 to drive the stirring rod 407 to move downward, so that the stirring rod 407 is inserted into the ice cube, and at the same time, the dial plate 409 on one side is also inserted into the ice cube in the cooling tank, and the rotating motor 303 is started to make the rotating seat 301 drive the stirring rod 407 to move downward. The cooling cylinder 107 is driven to rotate, and the ice cubes and the stirring rod 407 are rotated relative to each other, so that the ice cubes can be stirred. During the stirring, the stirring rod 407 on one side is responsible for stirring the ice cubes, and the stirring rod 407 on the other side, under the action of the inclined dial plate 409, dials the ice cubes to one side of the sample container 108, so that the ice cubes fit closely with the sample container 108, and the stacking height of the ice cubes is increased, and the contact area between the ice cubes and the sample container 108 is increased to ensure the cooling efficiency of the ice cubes on the sample solution. Therefore, in the process of cell disruption, the present invention utilizes the coordination of the structure between the rotating component 3 and the cleaning component 4 to achieve the stirring and dialing of the ice cubes, increase the contact area between the ice cubes and the sample container 108, and ensure the cooling efficiency of the sample solution.

[0033] At the same time, the cooling cylinder 107 is utilized to drive the rotation of the sample container 108 to realize the rotation of the sample solution, so that the bubbles generated in the sample solution under the vibration of the horn 103 are more evenly distributed, thereby increasing the contact area between the ultrasonic wave and the sample, so that the energy of the ultrasonic wave is more effectively transmitted to the sample, and the crushing efficiency is improved. At the same time, the shear force and impact force on the cell wall are increased, and the cell wall of the red algae cells is further promoted to rupture, thereby releasing more phycoerythrin 545 and accelerating the heat exchange between the middle of the sample solution and the ice cube.

[0034] The above descriptions are merely embodiments of the present invention and are not intended to limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made using the contents of the present invention specification and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. An extraction device for phycoerythrin 545 extract based on ultrasonic crushing method, comprising a soundproof box (1), characterized in that: An ultrasonic generating module (102) is provided on the top of the soundproof box (1), and a horn (103) is provided at the bottom output end of the ultrasonic generating module (102); A limiting groove (104) is provided on the inner side of the soundproof box (1), a limiting plate (105) is slidably connected in the limiting groove (104), a supporting plate (106) is provided at the end of the limiting plate (105), a cooling cylinder (107) is provided above the supporting plate (106), and a sample container (108) is detachably connected to the middle of the cooling cylinder (107); A lifting assembly (2) for driving the support plate (106) to lift and lower is provided on the inner bottom side of the soundproof box (1), a rotating assembly (3) for driving the cooling cylinder (107) to rotate is provided on the top of the support plate (106), and a cleaning assembly (4) for cleaning the horn (103) is provided on the inner side of the soundproof box (1); The cleaning assembly (4) comprises a slide rail (401), the interior of the slide rail (401) being rotatably connected with a bidirectional screw rod (402), both sides of the bidirectional screw rod (402) being threadedly sleeved with support arms (403), the ends of the support arms (403) on both sides being provided with fixed rods (404) on opposite sides, the fixed rods (404) on both sides being provided with arc-shaped seats (405) on opposite ends, and the inner side of the arc-shaped seats (405) being provided with arc-shaped cleaning portions (406).

2. The extraction device of phycoerythrin 545 extract based on ultrasonic crushing method according to claim 1 is characterized in that: A sound insulation layer is provided inside the sound insulation box (1), a sealed door (101) is movably connected to the front side of the sound insulation box (1), a sound insulation glass is embedded in the middle of the sealed door (101), the annular space between the sample container (108) and the cooling cylinder (107) forms a cooling groove, and a temperature sensing probe (109) is provided on the inner top of the sound insulation box (1).

3. The extraction device of phycoerythrin 545 extract based on ultrasonic crushing method according to claim 1, characterized in that: The lifting assembly (2) comprises a guide rail (201), a stud (203) is rotatably connected inside the guide rail (201), a sliding seat (202) is threadedly sleeved on the stud (203), a movable rod (205) is movably connected to the top of the sliding seat (202), the top of the movable rod (205) is movably connected to the bottom of the support plate (106), a lifting motor (204) is provided at the end of the guide rail (201), and the output shaft of the lifting motor (204) is fixedly connected to the end of the stud (203); The guide rail (201) is arranged on the inner bottom surface of the sound insulation box (1), and the sliding seat (202) is slidably connected to the guide rail (201).

4. The extraction device of phycoerythrin 545 extract based on ultrasonic crushing method according to claim 1, characterized in that: The rotating assembly (3) comprises a rotating seat (301) and a rotating motor (303); a gear ring (302) is fixedly sleeved on the circumferential surface of the rotating seat (301); and a rotating gear (304) is meshed on the side surface of the gear ring (302).

5. The extraction device of phycoerythrin 545 extract based on ultrasonic crushing method according to claim 4 is characterized in that: The rotating seat (301) is rotatably connected to the top of the support plate (106), the top of the rotating seat (301) is fixedly connected to the bottom of the cooling cylinder (107), the rotating motor (303) is arranged on the limiting plate (105), and the output shaft of the rotating motor (303) is fixedly connected to the rotating gear (304).

6. The extraction device of phycoerythrin 545 extract based on ultrasonic crushing method according to claim 1, characterized in that: The support arm (403) is slidably connected to the slide rail (401), and stirring rods (407) are provided at the bottom of the arc-shaped seats (405) on both sides, wherein a dial plate (409) is obliquely provided at the bottom end of the stirring rod (407) on one side.

7. The extraction device of phycoerythrin 545 extract based on ultrasonic crushing method according to claim 6, characterized in that: An electric telescopic rod (408) is provided on the inner top side of the soundproof box (1); the telescopic end of the electric telescopic rod (408) is fixedly connected to the top of the slide rail (401); a drive motor (410) is provided at the end of the slide rail (401); and the output shaft of the drive motor (410) is fixedly connected to the end of the bidirectional screw rod (402).

8. The extraction device of phycoerythrin 545 extract based on ultrasonic crushing method according to claim 1, characterized in that: The slide rail (401) is slidably connected to the inner side surface of the sound insulation box (1), and threads with opposite spiral directions are symmetrically provided on both sides of the bidirectional screw (402). The centers of the arc-shaped cleaning portion (406) and the arc-shaped seat (405) coincide with the central axis of the amplitude transformer (103), and the arc-shaped cleaning portion (406) and the arc-shaped seat (405) are bonded by Velcro.

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