Automatic erythrocyte suspension preparation device and erythrocyte suspension preparation method

Abstract

The invention relates to the technical field of red blood cell suspension automatic preparation, in particular to a red blood cell suspension automatic preparation device and a red blood cell suspension preparation method. The red blood cell suspension automatic preparation device comprises a sleeve, wherein a contact switch is arranged at the inner bottom of the sleeve; the two sides of the outer side wall of the sleeve are connected with a plurality of laser emitters and a plurality of light sensation receivers correspondingly. The test tube is arranged in the sleeve in a sleeving manner and is detachably connected with the sleeve; a tube opening of the test tube is detachably connected with a normal saline adding assembly; the test tube is used for placing packed red blood cells or prepared red blood cell suspension; the industrial personal computer is electrically connected with the contact switch, the laser transmitter, the light sensation receiver and the normal saline adding assembly and used for receiving signals of the contact switch, controlling the laser transmitter and the light sensation receiver and controlling the running state of the normal saline adding assembly. According to the device provided by the invention, the suspension with required erythrocyte concentration can be accurately and quickly obtained.

Description

Technical Field

[0001] The present invention relates to the technical field of automatic configuration of red blood cell suspension, and in particular to an automatic configuration device of red blood cell suspension and a configuration method of red blood cell suspension. Background Art

[0002] In clinical experiments, such as ABO blood typing, Rh blood typing, cross-matching test, titer determination of anti-A and anti-B blood typing reagents, stability and affinity evaluation test, etc., the red blood cell suspension used is mainly composed of red blood cells and normal saline, which is obtained by taking an appropriate amount of hematocrit and diluting it with normal saline. The red blood cell concentration of the red blood cell suspension is of great significance for both clinical blood transfusion and experimental research. In experimental research, the concentration of the red blood cell suspension is crucial to the accuracy and repeatability of the experimental results. For example, in the hemolysis experiment, red blood cell suspensions of different concentrations may produce different experimental results. In the red blood cell osmotic fragility experiment, the concentration of the red blood cell suspension is very important for evaluating the sensitivity of red blood cells to hypotonic solutions, which helps to understand the stability and osmotic pressure regulation mechanism of red blood cells. The preparation method of the red blood cell suspension, including the required reagents, equipment and steps, requires precise operation to ensure that the final red blood cell concentration meets the experimental or clinical needs. Therefore, how to quickly obtain a red blood cell suspension with an accurate red blood cell concentration is crucial to ensure the safety and effectiveness of clinical blood transfusion and the accuracy of experimental research.

[0003] In the prior art, the preparation of a suspension with a certain red blood cell concentration is mostly done manually, where an appropriate amount of hematocrit is manually taken and a certain amount of saline is added and mixed according to experience. At present, some patents mention the amount of saline automatically added by an automatic configuration device. However, these automatic configuration devices only add the amount of saline based on experience or theory. Due to factors such as errors during the addition process, the final concentration of the configured red blood cell suspension is uncertain. Summary of the invention

[0004] The technical problem to be solved by the present invention is to overcome the defect of inaccurate concentration of red blood cell suspension in the prior art, and to provide a device and method for automatically configuring a red blood cell suspension.

[0005] In order to solve the above technical problems, the present invention adopts the following technical solutions.

[0006] A first aspect of the present invention provides an automatic red blood cell suspension configuration device, comprising:

[0007] A sleeve, wherein the inner bottom of the sleeve is provided with a contact switch; and the side walls of the sleeve are respectively connected with a plurality of laser transmitters and a plurality of light-sensing receivers;

[0008] A test tube is sleeved in the sleeve and detachably connected to the sleeve; a normal saline adding component is detachably connected to the mouth of the test tube; the test tube is used to place packed red blood cells or prepared red blood cell suspension;

[0009] The industrial computer is electrically connected to the contact switch, the laser transmitter, the photosensitive receiver and the physiological saline addition component, and is used to receive signals from the contact switch, control the laser transmitter and the photosensitive receiver, and control the operating state of the physiological saline addition component.

[0010] In some technical schemes of the present invention, the physiological saline addition component includes: a physiological saline tank, a metering pump and a drainage tube; one end of the drainage tube is connected to the physiological saline tank, and the other end is detachably connected to the test tube opening of the test tube, and the drainage tube is also detachably connected to the metering pump, and the metering pump is located between the physiological saline tank and the test tube; when the metering pump is working, the physiological saline in the physiological saline tank is transported to the test tube through the drainage tube.

[0011] In some technical solutions of the present invention, the laser transmitter and the light receiver are symmetrically arranged along the central axis of the test tube.

[0012] In some technical solutions of the present invention, the number of the laser transmitters and the number of the light-sensing receivers are the same.

[0013] In some technical solutions of the present invention, the length of the test tube is 7 cm to 10 cm.

[0014] In some technical solutions of the present invention, the number of the laser transmitters and the number of the light-sensing receivers are the same.

[0015] In some technical solutions of the present invention, the number of the laser transmitters and light receivers is 6 to 12.

[0016] In some technical solutions of the present invention, the industrial computer is also connected to a switch output module and a signal input module.

[0017] In some technical solutions of the present invention, the switch output module is used to control the operation of the metering pump and the laser transmitter;

[0018] The signal input module is used to obtain output signals of the light sensing receiver and the contact switch.

[0019] The second aspect of the present invention also provides a method for preparing a red blood cell suspension, comprising: preparing a red blood cell suspension using the automatic red blood cell suspension preparation device.

[0020] Compared with the prior art, the present invention has the following beneficial effects: through the automatic configuration device for red blood cell suspension provided by the present invention, after the required concentration is input into the industrial control computer, the test tube containing the packed red blood cells is placed in the sleeve, the industrial control computer controls the operation of the device, and the amount of physiological saline is controlled based on the concentration feedback closed loop, so that the red blood cell suspension with precise concentration can be obtained quickly, and the error caused by manual configuration of the red blood cell suspension and the error caused by the automatic configuration device adding the amount of physiological saline through experience or theory can be avoided.

[0021] The automatic red blood cell suspension configuration device provided by the present invention is used to configure the red blood cell suspension, which is easy to operate and can produce a red blood cell suspension with a precise concentration. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a schematic diagram of the structure of the automatic configuration device for red blood cell suspension provided by the present invention.

[0023] Figure numerals: 1-sleeve, 2-contact switch, 3-laser transmitter, 4-photosensitive receiver, 5-test tube, 6-industrial computer, 7-physiological saline tank, 8-metering pump, 9-drainage tube, 10-switch output module, 11-signal input module. DETAILED DESCRIPTION

[0024] The present invention is described in detail below in conjunction with specific examples, but it should not be construed as limiting the present invention. Unless otherwise specified, the technical means used in the following examples are conventional means well known to those skilled in the art, and the materials, reagents, etc. used in the following examples, unless otherwise specified, can be obtained from commercial sources.

[0025] In the red blood cell osmotic fragility experiment, the concentration of the red blood cell suspension is very important for evaluating the sensitivity of red blood cells to hypotonic solutions, which helps to understand the stability and osmotic pressure regulation mechanism of red blood cells. The preparation method of the red blood cell suspension, including the required reagents, equipment and steps, requires precise operation to ensure that the final red blood cell concentration meets the experimental or clinical needs. In the prior art, the preparation of suspensions with a certain red blood cell concentration is mostly done manually, manually taking an appropriate amount of hematocrit, and adding a certain amount of physiological saline according to experience to mix. At present, some patents mention the amount of physiological saline automatically added by an automatic configuration device. However, these two methods only add the amount of physiological saline through experience or theory. Due to factors such as errors in the addition process, the final concentration of the configured red blood cell suspension is uncertain.

[0026] The present invention provides an automatic configuration device for a red blood cell suspension, comprising: a sleeve 1, wherein a contact switch 2 is configured at the inner bottom of the sleeve 1; a plurality of laser emitters 3 and a plurality of photosensitive receivers 4 are respectively connected to the side walls of the sleeve 1; a test tube 5 is sleeved in the sleeve 1 and detachably connected to the sleeve 1; a normal saline adding component is detachably connected to the tube mouth of the test tube 5; the test tube 5 is used for placing packed red blood cells or prepared red blood cell suspension; an industrial computer 6 is electrically connected to the contact switch 2, the laser emitter 3, the photosensitive receiver 4 and the normal saline adding component, and is used for receiving signals from the contact switch 2, controlling the laser emitter 3 and the photosensitive receiver 4, and controlling the operating state of the normal saline adding component.

[0027] After the test tube 5 is placed in the sleeve 1, it touches the contact switch 2, the switch is turned on, and the industrial computer 6 receives the signal from the contact switch 2, and controls the laser transmitter 3 and the light-sensing receiver 4 to work. The industrial computer 6 can obtain the height reached by the packed red blood cells in the test tube according to the signal changes of each light tube receiver 4, and the current amount of red blood cell overload can be calculated according to the cross-sectional area of ​​the test tube 5 and displayed in the industrial computer 6. The industrial computer 6 calculates the required amount of physiological saline according to the calculation formula (physiological saline amount = amount of packed red blood cells / required red blood cell concentration-amount of packed red blood cells) based on the amount of packed red blood cells and the required red blood cell concentration input by the user, and then the industrial computer 6 controls the operation of the physiological saline adding component to add the required physiological saline to the test tube 5, thereby realizing the preparation of a red blood cell suspension of a certain concentration. Compared with the prior art, the automatic configuration device for red blood cell suspension provided by the present invention can obtain a red blood cell suspension of a precise concentration more quickly and accurately.

[0028] Example 1

[0029] An automatic red blood cell suspension configuration device, comprising:

[0030] A sleeve 1, the inner bottom of which is provided with a contact switch 2; a plurality of laser transmitters 3 and a plurality of light receivers 4 are respectively connected to both sides of the outer wall of the sleeve 1;

[0031] The test tube 5 is sleeved in the sleeve 1 and detachably connected to the sleeve 1; the mouth of the test tube 5 is detachably connected to a physiological saline addition component; the test tube 5 is used to place packed red blood cells or prepared red blood cell suspension;

[0032] The industrial computer 6 is electrically connected to the contact switch 2, the laser transmitter 3, the photosensitive receiver 4 and the saline addition component, and is used to receive signals from the contact switch 2, control the laser transmitter 3 and the photosensitive receiver 4, and control the operating state of the saline addition component.

[0033] In the present invention, the laser emitters 3 and the photosensitive receivers 4 are symmetrically arranged along the central axis of the test tube 5. And the number of the laser emitters 3 and the photosensitive receivers 4 is the same.

[0034] The physiological saline addition component includes: the physiological saline addition component includes: a physiological saline tank 7, a metering pump 8 and a drainage tube 9; one end of the drainage tube 9 is connected to the physiological saline tank 7, and the other end is detachably connected to the test tube opening of the test tube 5, and the drainage tube 9 is also detachably connected with the metering pump 8, and the metering pump 8 is located between the physiological saline tank 7 and the test tube 5; under the action of the metering pump 8, the physiological saline in the physiological saline tank 7 is transported to the test tube 5 through the drainage tube 9.

[0035] The industrial computer 6 is also connected to a switch output module 10 and a switch output module 11;

[0036] The switch output module 10 is used to control the operation of the metering pump 8 and the laser transmitter 3;

[0037] Switching output module 11 The switching output module 11 is used to obtain output signals of the light sensing receiver 4 and the contact switch 2 .

[0038] Automatic red blood cell suspension configuration method and device workflow:

[0039] According to the experimental needs, the user adds a certain amount of packed red blood cells to the test tube 5, and then inputs the required red blood cell concentration into the industrial computer 6. After the test tube 5 with packed red blood cells is placed in the sleeve 1, the test tube 5 connects the contact switch 2, and the industrial computer 6 can receive the signal that the contact switch 2 is turned on. The industrial computer 6 controls the opening of each laser transmitter through the switch output module 10. The output signal size of each photosensitive receiver on the signal receiving device can be obtained by the switch output module 11 and sent to the industrial computer 6. The industrial computer 6 can obtain the height reached by the packed red blood cells in the test tube according to the changes in the signals of each photosensitive receiver, and the current red blood cell overload can be calculated according to the cross-sectional area of ​​the test tube and displayed in the industrial computer 6. The industrial computer 6 calculates the required amount of saline according to the formula (amount of saline = amount of packed red blood cells / required red blood cell concentration-amount of packed red blood cells) based on the amount of packed red blood cells and the required red blood cell concentration input by the user, and then calculates the running time of the metering pump according to the working flow of the metering pump (running time = required amount of saline / working flow of the metering pump) and controls the metering pump to initially inject a small amount of saline into the test tube through the switch output module. After the initial addition of saline, the industrial computer 6 obtains the current red blood cell suspension concentration according to the intensity of the signal of each light-sensing receiver and the experimental relationship curve between the signal intensity and the red blood cell suspension concentration, compares it with the required red blood cell concentration input by the user, and fine-tunes the metering pump to slowly add saline until the red blood cell suspension concentration in the test tube is consistent with the concentration required by the user.

[0040] Through this experimental device, a suspension with a required red blood cell concentration can be obtained accurately and quickly.

[0041] In the present invention, the contact switch 2 was purchased from Suqian Tongte E-commerce Co., Ltd., model G5W11 small micro switch. The laser transmitter 3 was purchased from Jining Yuze Industrial Technology Co., Ltd., model UTI K. The light-sensitive receiver was purchased from Shenzhen Sichi Electronics Co., Ltd., 5MM photoresistor photoelectric detection element sensor switch. The switch output module 10 was purchased from Beijing Altai Technology, model DAM3018 / 3944. The switch output module 11 was purchased from Beijing Altai Technology, model DAM3128 analog quantity to 485 analog quantity acquisition module. The industrial computer 6 was purchased from Yanjiang Technology, model YJMPPC-190.

[0042] Although the preferred embodiments of the present invention have been described, those skilled in the art may make other changes and modifications to these embodiments once they have learned the basic creative concept. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention.

[0043] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. An automatic red blood cell suspension configuration device, characterized in that: include: A sleeve (1), wherein a contact switch (2) is arranged at the inner bottom of the sleeve (1); and a plurality of laser transmitters (3) and a plurality of light-sensing receivers (4) are respectively connected to the side walls of the sleeve (1); The test tube (5) is sleeved in the sleeve (1) and is detachably connected to the sleeve (1); the mouth of the test tube (5) is detachably connected to a physiological saline addition component; the test tube (5) is used to place packed red blood cells or prepared red blood cell suspension; The industrial computer (6) is electrically connected to the contact switch (2), the laser transmitter (3), the light receiver (4) and the physiological saline addition component, and is used to receive signals from the contact switch (2), control the laser transmitter (3) and the light receiver (4), and control the operating state of the physiological saline addition component.

2. The automatic red blood cell suspension configuration device according to claim 1, characterized in that: The physiological saline addition component comprises: a physiological saline tank (7), a metering pump (8) and a drainage tube (9); one end of the drainage tube (9) is connected to the physiological saline tank (7), and the other end is detachably connected to the test tube opening of the test tube (5); the drainage tube (9) is also detachably connected to the metering pump (8), and the metering pump (8) is located between the physiological saline tank (7) and the test tube (5); when the metering pump (8) is in operation, the physiological saline in the physiological saline tank (7) is transported to the test tube (5) through the drainage tube (9).

3. The automatic red blood cell suspension configuration device according to claim 1, characterized in that: The laser emitter (3) and the light receiver (4) are symmetrically arranged along the central axis of the test tube (5).

4. The automatic red blood cell suspension configuration device according to claim 3, characterized in that: The length of the test tube (5) is 7 cm to 10 cm.

5. The automatic red blood cell suspension configuration device according to claim 3, characterized in that: The number of the laser transmitters (3) and the number of the light-sensing receivers (4) are the same.

6. The automatic red blood cell suspension configuration device according to claim 5, characterized in that: The number of the laser transmitters (3) and the light-sensing receivers (4) is 6 to 12.

7. The automatic red blood cell suspension configuration device according to claim 1, characterized in that: The industrial computer is electrically connected to a switch output module (10) and a signal input module (11).

8. The automatic red blood cell suspension configuration device according to claim 7, characterized in that: The switch output module (10) is used to control the operation of the metering pump (8) and the laser transmitter (3); The signal input module (11) is used to obtain output signals of the light sensing receiver (4) and the contact switch (2).

9. A method for preparing a red blood cell suspension, characterized in that: include: The red blood cell suspension is prepared using the automatic red blood cell suspension preparation device as described in any one of claims 1 to 8.

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