Active peptide compound for improving sleep quality, preparation method and application

Abstract

The invention discloses an active peptide compound for improving sleep quality as well as a preparation method and application thereof, and relates to the technical field of polypeptide compounds. The active peptide compound is prepared from the following components in percentage by weight: 22.73 percent to 37.04 percent of collagen cyclic dipeptide, 22.73 percent to 37.04 percent of cow milk source sleep promoting peptide, 28.57 percent to 45.45 percent of tea leaf theanine and 6.25 percent to 14.29 percent of gamma-aminobutyric acid. According to the active peptide compound capable of improving the sleep quality, the preparation method and the application, theanine is used as a surfactant and a stabilizer to be combined with active peptides capable of improving the sleep quality, such as cow milk-derived bioactive peptides, on one hand, the active peptide compound capable of improving the sleep quality is provided, and on the other hand, the active peptide compound capable of improving the sleep quality is provided. And the dosage of additives in the cow milk-derived bioactive peptide sleep quality improving products can be reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of polypeptide complexes, and in particular to an active peptide complex for improving sleep quality, a preparation method and an application thereof. Background Art

[0002] Currently, sleep problems have become one of the hottest issues in the world. At present, more and more sleep-promoting products are appearing on the market, including barbiturates and zolpidem drugs. However, long-term use of these synthetic substances can lead to some side effects, including amnesia and ataxia. In order to overcome the side effects of these drugs, a new class of natural products with sleep-improving effects has attracted widespread attention.

[0003] Bovine milk-derived bioactive peptides refer to free active peptides in bovine milk and active peptides released from milk proteins after enzymatic hydrolysis. They have multiple activities, such as immunomodulation, antibacterial, antioxidant, blood pressure lowering, and sleep promotion, and have been widely used in the research of various functional foods. Studies have found that bovine milk-derived sleep-promoting peptides have the characteristics of promoting sleep and have the effect of promoting sleep in mice induced by sodium pentobarbital. At the same time, it has the effect of alleviating related symptoms for people with sleep disorders, has no side effects on people with normal sleep, and also has analgesic, immune-enhancing and other functions. The sleep quality index scores of subjects who have taken bovine milk-derived sleep-promoting peptides for a long time have significantly improved, and their sleep quality has been improved.

[0004] The bioactive peptides from bovine milk have poor stability in solution and are easy to aggregate to form precipitates or flocs, which is not conducive to production and storage.

[0005] Gamma-aminobutyric acid (GABA) acts as a molecular signal as an inhibitory neurotransmitter. It is produced by glutamate under the action of glutamate decarboxylase, synthesized at the nerve endings of the presynaptic membrane, secreted into the synaptic cleft under the action of calcium ions, reaches the postsynaptic membrane, binds to receptors, causes the Cl ion channel to open, produces hyperpolarization, regulates the activity of neurons, and has a certain effect on epilepsy, convulsions, Parkinson's disease and other mental illnesses. Related studies have pointed out that after mice were gavaged with a certain amount of GABA and 5-hydroxytryptamine, the transcription level of GABA-A receptors in the cerebral cortex of the fluorescent quantitative PCR line was 1.37 times higher than that of the control group, suggesting that deep GABA can affect the GABA content level and its receptor expression in the central nervous system through the vagus nerve pathway in the brain-gut axis, thereby playing a role in improving sleep.

[0006] The structure of theanine is very similar to that of glutamate, and it can competitively bind to glutamate receptors in the nervous system. Studies have shown that theanine can promote the release of dopamine in the brain, especially in the striatum. After that, part of theanine will be broken down into glutamate and enter the blood circulation, and the other part will become the raw material for synthesizing γ-aminobutyric acid.

[0007] A casein hydrolysate bioactive peptide composition and a preparation method thereof (application number: 202311259420.7), the casein hydrolysate bioactive peptide composition comprises, based on 100 parts by mass, 0.001-2 parts of casein hydrolysate bioactive peptide, 0.01-5 parts of stabilizer, 0-5 parts of osmotic pressure regulator, pH regulator, and the remainder is solvent; the pH regulator adjusts the pH of the casein hydrolysate bioactive peptide composition to 3-9. The invention provides the effect of improving sleep, can shorten the time to fall asleep, and prolong sleep time; the composition can be applied to food, daily necessities or medicines; the provided casein hydrolysate bioactive peptide composition has high biological activity and good physical and chemical stability.

[0008] However, the functional substance for improving sleep in the casein hydrolysate bioactive peptide composition is relatively single, and a variety of additives are added to stabilize the casein hydrolysate bioactive peptide. Summary of the invention

[0009] To solve the above problems, the purpose of the present invention is to provide an active peptide complex for improving sleep quality, a preparation method and an application thereof, and theanine is used as a surfactant and a stabilizer in combination with active peptides such as bovine milk-derived bioactive peptides that can improve sleep quality. On the one hand, an active peptide complex for improving sleep quality is provided, and on the other hand, the amount of additives in bovine milk-derived bioactive peptide products for improving sleep quality can be reduced.

[0010] To achieve the above objectives, on the one hand, the present invention provides an active peptide complex for improving sleep quality, comprising the following ingredients: collagen cyclic dipeptide, bovine milk-derived sleep-promoting peptide, tea theanine, and γ-aminobutyric acid.

[0011] Preferably, the weight ratio of each component is: collagen cyclic dipeptide 22.73%~37.04%, bovine milk-derived sleep-promoting peptide 22.73%~37.04%, tea theanine 28.57%~45.45%, and γ-aminobutyric acid 6.25%~14.29%.

[0012] Preferably, the weight ratio of each component is: 31.25% collagen cyclic dipeptide, 31.25% bovine milk-derived sleep-promoting peptide, 31.25% tea theanine, and 6.25% gamma-aminobutyric acid.

[0013] Preferably, the weight ratio of each component is: 22.73% collagen cyclic dipeptide, 22.73% bovine milk-derived sleep-promoting peptide, 45.45% tea theanine, and 9.09% gamma-aminobutyric acid.

[0014] Preferably, the weight ratio of each component is: 37.04% collagen cyclic dipeptide, 18.52% bovine milk-derived sleep-promoting peptide, 37.04% tea theanine, and 7.41% gamma-aminobutyric acid.

[0015] Preferably, the weight ratio of each component is: 28.57% collagen cyclic dipeptide, 28.57% bovine milk-derived sleep-promoting peptide, 28.57% tea theanine, and 14.29% gamma-aminobutyric acid.

[0016] On the other hand, the present invention provides a method for preparing the active peptide complex for improving sleep quality as described above, comprising the following steps: (1) Weigh the components of the active peptide complex according to the ratio and set aside; (2) Add collagen cyclic dipeptide and bovine milk-derived sleep-promoting peptide to tea theanine, stir evenly to obtain a mixed solution; (3) Add γ-aminobutyric acid to the mixed solution in step (2), stir evenly, then slowly add the solvent and stir until all the raw materials are fully dissolved.

[0017] Preferably, the solvent is water, and the volume of the solvent is measured according to the final volume to be prepared.

[0018] On the other hand, the present invention provides a use of the active peptide complex for improving sleep quality as described above in the preparation of protein drinks and / or medicines.

[0019] Preferably, the active peptide complex for improving sleep quality further comprises food additives when preparing protein drinks and / or medicines, including: ① 0~4% by weight of sweeteners, sugar polymers and / or flavorings, which is adjusted according to the sweetness of the beverage.

[0020] ②0~4% by weight of osmotic pressure regulator: sodium chloride, phosphate, citrate, glycerol, propylene glycol, mannitol, sorbitol, glucose, fructose, lactose or trehalose; ③ 0-4% by weight of pH adjuster: citric acid, citrate, acetic acid, acetate, phosphoric acid, phosphate, sodium hydroxide, hydrochloric acid, lactic acid and / or maleic acid; ④Preservatives or antibacterial agents.

[0021] The active peptide complex for improving sleep quality, preparation method and application of the present invention have the following beneficial effects: (1) Theanine is combined with active peptides such as bovine milk-derived bioactive peptides that can improve sleep quality to provide an active peptide complex that can improve sleep quality, shorten the time it takes to fall asleep, and prolong sleep time; (2) Theanine can be used as a surfactant and stabilizer, which can reduce the amount of additives used in milk-derived bioactive peptide products for improving sleep quality, and the chemical properties of the prepared products are stable; (3) The active peptide complex for improving sleep quality of the present invention can be applied to the preparation of protein drinks or medicines for improving sleep quality. The detection shows that the active peptide complex has no direct sleep effect and will not cause harm to the human body. It can also promote the natural sleep of the user and has a good application prospect.

[0022] The technical solution of the present invention is further described in detail below through the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for use in the description of the embodiments of the present invention will be briefly introduced below. Obviously, the accompanying drawings in the following description are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative labor.

[0024] Figure 1 The present invention is a flow chart for configuring the active peptide complex for improving sleep quality. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical scheme and advantages disclosed in the embodiments of the present invention more clear, the embodiments of the present invention are further described in detail in conjunction with the embodiments below. It should be understood that the specific embodiments described herein are only used to explain the embodiments of the present invention and are not intended to limit the embodiments of the present invention. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative work belong to the scope of protection of this application.

[0026] In the present invention, unless otherwise specified, all equipment and raw materials can be purchased from the market or are commonly used in the industry. The methods in the following embodiments are all conventional methods in the art unless otherwise specified.

[0027] Embodiment 1 An active peptide complex for improving sleep quality, comprising 100 mg of collagen cyclic dipeptide, 100 mg of bovine milk-derived sleep-promoting peptide, 100 mg of tea theanine, 20 mg of gamma-aminobutyric acid, and adding purified water to 20 g.

[0028] Collagen cyclic dipeptide is cyclic (4-hydroxyproline-glycine) dipeptide, which is a commercially available product and has the following structural formula: .

[0029] The preparation method comprises: (1) Add collagen cyclic dipeptide and milk-derived sleep-promoting peptide to tea theanine and stir evenly; (2) Add γ-aminobutyric acid to the mixed solution in step (1), stir evenly, then slowly add purified water and mix well. Observe that all the raw materials are fully dissolved.

[0030] Embodiment 2 An active peptide complex for improving sleep quality is prepared in the same manner as in Example 1, and the raw materials include: 50 mg of collagen cyclic dipeptide, 50 mg of bovine milk-derived sleep-promoting peptide, 100 mg of tea theanine, 20 mg of γ-aminobutyric acid, and purified water is added to 20 g. All raw materials are observed to be fully dissolved.

[0031] Embodiment 3 An active peptide complex for improving sleep quality, prepared in the same manner as in Example 1, includes raw materials: 100 mg of collagen cyclic dipeptide, 50 mg of bovine milk-derived sleep-promoting peptide, 100 mg of tea theanine, 20 mg of γ-aminobutyric acid, and purified water is added to 20 g. All raw materials are observed to be fully dissolved.

[0032] Embodiment 4 An active peptide complex for improving sleep quality, prepared in the same manner as in Example 1, includes: 100 mg of collagen cyclic dipeptide, 100 mg of bovine milk-derived sleep-promoting peptide, 100 mg of tea theanine, 50 mg of γ-aminobutyric acid, and purified water is added to 20 g. All the raw materials are observed to be fully dissolved.

[0033] Embodiment 5 An active peptide complex for improving sleep quality, the raw materials include: 100 mg of collagen cyclic dipeptide, 100 mg of bovine milk-derived sleep-promoting peptide, 50 mg of γ-aminobutyric acid, and purified water to 20 g. Collagen cyclic dipeptide cannot be fully dissolved in purified water, proving that theanine plays the role of a surfactant and stabilizer.

[0034] Embodiment 6 An active peptide complex for improving sleep quality, comprising: 200 mg of sleep-promoting peptide from bovine milk, 50 mg of gamma-aminobutyric acid, and purified water to 20 g.

[0035] Embodiment 7 An active peptide complex for improving sleep quality, comprising: 100 mg of sleep-promoting peptide from bovine milk, 100 mg of theanine from tea leaves, 50 mg of gamma-aminobutyric acid, and purified water to 20 g.

[0036] Embodiment 8 A protein beverage formula containing an active peptide complex for improving sleep quality, comprising the following ingredients: collagen cyclic dipeptide, bovine milk-derived sleep-promoting peptide, tea theanine, and gamma-aminobutyric acid.

[0037] The weight ratio of each component is: collagen cyclic dipeptide 22.73%~37.04%, bovine milk-derived sleep-promoting peptide 22.73%~37.04%, tea theanine 28.57%~45.45%, and γ-aminobutyric acid 6.25%~14.29%.

[0038] The weight ratio of collagen cyclic dipeptide to bovine milk-derived sleep-promoting peptide and tea theanine is 1~2:1~2:1~2.

[0039] The weight ratio of tea theanine to γ-aminobutyric acid is 5:1~2.5.

[0040] Collagen cyclic dipeptide is isolated from bovine bones, and milk-derived sleep-promoting peptide is isolated from bovine milk. Collagen cyclic dipeptide, milk-derived sleep-promoting peptide, tea theanine, and γ-aminobutyric acid are all conventional products.

[0041] The formula also includes food additives, including: ① 0~4% by weight of sweeteners, sugar polymers and / or flavorings, which is adjusted according to the sweetness of the beverage.

[0042] ②0~4% by weight of osmotic pressure regulator: sodium chloride, phosphate, citrate, glycerol, propylene glycol, mannitol, sorbitol, glucose, fructose, lactose or trehalose; ③ 0-4% by weight of pH adjuster: citric acid, citrate, acetic acid, acetate, phosphoric acid, phosphate, sodium hydroxide, hydrochloric acid, lactic acid and / or maleic acid; ④Preservatives or antibacterial agents.

[0043] A method for preparing a protein beverage containing an active peptide complex for improving sleep quality: (1) Dissolve the additives except the pH adjuster in 10%~50% solvent.

[0044] (2) Add collagen cyclic dipeptide and milk-derived sleep-promoting peptide to tea theanine and stir evenly.

[0045] (3) Add γ-aminobutyric acid to the mixed solution in step (2), stir evenly, then slowly add 10% of solvent and stir until all the raw materials are fully dissolved.

[0046] (4) The solution in step (1) is mixed with the solution in step (3), a pH adjuster is added to adjust the pH to 3-9, and then the remaining solvent is added and mixed evenly.

[0047] The solvent is water or an organic solvent; the organic solvent is selected from at least one of alcohol organic solvents.

[0048] Test Case Animal experiments were conducted on the active peptide complexes in Examples 1 to 7 to verify their effects on improving sleep quality.

[0049] (1) Animal husbandry and grouping 180 SPF Balb-c healthy male mice were housed in an animal laboratory at room temperature (25±2)℃, relative humidity 55%±2%, 12h / 12h light / dark, free access to food and water. They were divided into 3 groups according to body weight, 60 mice in each group. There was no statistical difference in body weight among the groups.

[0050] Among them, Group 1 conducted an experiment to extend the sleep time of sodium pentobarbital; Group 2 conducted an experiment on the subthreshold dose hypnosis of sodium pentobarbital; Group 3 conducted an experiment on the sleep latency of sodium barbital; and the above experiments were also conducted with direct sleep experiments. Because mice are more active at night than during the day, animal intervention and experiments were carried out at 8 o'clock in the evening to eliminate the impact of changes in animal activity status on the experiment. The reagents used in the experiment were all commercially available, and sodium pentobarbital and sodium barbital were analytically pure.

[0051] (2) Animal intervention 60 mice were tested in each group and fed adaptively for 3 days. Before the experiment, the initial body weight of the mice was weighed and divided into 6 groups according to the body weight of the mice, namely a blank control group (purified water) and 5 test groups to which the active peptide complexes in Examples 1 to 5 were applied, with 10 mice in each group. Each group of animals was gavaged once a day for 30 days. The body weight of the mice was measured once a week and the gavage dose was adjusted.

[0052] (3) The experiment of prolonging the sleeping time of sodium pentobarbital was carried out with reference to the Technical Specifications for Inspection and Evaluation of Health Foods. 30 minutes after the animals were last gavaged with the sample, each group of mice was intraperitoneally injected with 50 mg / kg·bw sodium pentobarbital (ip) (a dose that causes 100% sleep in mice, which has been verified in previous experiments). The injection volume was 0.1 mL / 10 g·bw. The disappearance of the righting reflex of mice for more than 60 seconds was used as the criterion for falling asleep, and the time from the disappearance of the righting reflex to the recovery of the righting reflex of the mice was used as the sleeping time of the animals. The test substances were compared to see whether they prolong the sleeping time of the mice injected with sodium pentobarbital.

[0053] (4) The subthreshold dose hypnotic experiment of sodium pentobarbital was carried out with reference to the Technical Specifications for Inspection and Evaluation of Health Foods. 30 minutes after the animals were last gavaged with the test substance, each group of mice was intraperitoneally injected with 30 mg / kg·bw sodium pentobarbital (ip) (a subthreshold dose for mice to fall asleep at 10% to 20%), with an injection volume of 0.1 mL / 10 g·bw. The disappearance of the righting reflex of mice for more than 60 seconds was used as the criterion for falling asleep. The number of animals that fell asleep in each group within 30 minutes was recorded and the incidence of sleep was calculated.

[0054] Sleep incidence (%) = (number of animals falling asleep / number of animals in each group) × 100%.

[0055] To compare whether the test substances increase the sleep incidence of mice injected with subthreshold doses of sodium pentobarbital.

[0056] (5) The sleep latency test of barbiturate sodium was carried out with reference to the Technical Specifications for Inspection and Evaluation of Health Foods. 20 minutes after the animals were last gavaged with the test substance, each group of mice was intraperitoneally injected with 280 mg / kg·bw barbiturate sodium (ip) (100% sleep-inducing dose for mice), with an injection volume of 0.1 mL / 10 g·bw. The disappearance of the righting reflex of mice for more than 60 seconds was used as the sleep criterion, and the time from the injection of barbiturate sodium to the disappearance of the righting reflex was used as the sleep latency. The test substance was compared to see whether it could shorten the sleep latency of mice injected with barbiturate sodium.

[0057] (6) Direct sleep test Each group of animals was given the test substance every day, and the mice were observed to see whether they fell asleep within 1 hour after oral administration. The disappearance of the righting reflex of the animals for more than 60 seconds was considered as falling asleep. The direct hypnotic effects of the blank control group and each dose group of the formula product on mice were compared.

[0058] (7) Data processing Data were expressed as "mean ± standard deviation (X ± SD)". SPSS18.0 software was used for statistical analysis. Count data were analyzed using chi-square test, and measurement data were analyzed using one-way ANOWA or rank sum test. The significance level was P < 0.05.

[0059] Results Analysis (1) Effect of the active peptide complex formula on the body weight of mice The body weight of animals in the experiment of extending the sleeping time of sodium pentobarbital was analyzed, and the results are shown in Table 1: Table 1 Effects of active peptide complex on body weight of mice ; ; As shown in Table 1, there was no significant difference in the initial body weight of mice in each group before the experiment (P>0.05); after oral administration of different doses of the formula for 7, 14, 21, and 30 days, there was no significant difference in the body weight of mice in each group (P>0.05). During the experiment, the fur of mice in each group was shiny, and their diet and activities were normal. After the sample was administered, the feces of mice were moderately moist, and no diarrhea or loose stools were observed, indicating that the formula product had no significant effect on the weight of mice at the designed dose of this experiment.

[0060] (2) Effect of the active peptide complex formula in the example on the sleep time of mice induced by sodium pentobarbital The sleep time met the normality test and variance homogeneity test requirements after statistical analysis, and the data were statistically processed using one-way analysis of variance. The results are shown in Table 2: Table 2 Effects of active peptide complex on sleep time of mice induced by sodium pentobarbital ; ; Note: a indicates that there is a significant difference compared with the blank control group, P<0.01; b indicates that there is a significant difference compared with the fifth group of Example, P<0.01; c indicates that there is a significant difference compared with the seventh group of Example, P<0.01.

[0061] Compared with the model control group, the sleep time of mice treated with the active peptide complexes of Examples 1 to 5 showed significant differences, and the differences were statistically significant (Table 2, P<0.01).

[0062] Compared with the sleep time of the mice in the active peptide complex groups of Examples 1 to 5, only the sleep time of the mice in the group with increased γ-aminobutyric acid content in Example 4 was longer than that of the other groups, but there was no significant difference compared with the sleep time of the mice in the active peptide complex groups of Examples 1 to 3.

[0063] The sleeping time of the mice tested in Example 5 without theanine was significantly lower than that of the mice tested in the active peptide complexes of Examples 1 to 4, and significantly higher than that of the mice in the blank control group, indicating that theanine has the effect of improving sleep quality.

[0064] The sleep time of mice treated with the composition without theanine and collagen cyclic dipeptide in Example 6 increased compared with the sleep time of mice in the blank control group, but there was no significant difference between the blank control group and the composition, indicating that theanine and collagen cyclic dipeptide played the main role in the active peptide complex.

[0065] The sleep time of mice tested with the composition without collagen cyclic dipeptide in Example 7 was significantly lower than that of mice tested with the active peptide complexes in Examples 1 to 4, and was significantly higher than that of mice in the blank control group, indicating that collagen cyclic dipeptide has the effect of improving sleep quality.

[0066] (3) Effect of the active peptide complex formula in the example on the hypnotic effect of subthreshold dose of sodium pentobarbital The incidence of falling asleep in the blank control group was 20%, which met the modeling requirements of this experiment, indicating that the subthreshold hypnotic dose mouse model of sodium pentobarbital was successfully established. The exact probability method was used for statistical processing, and the results are shown in Table 3: Table 3 Effects of active peptide complex on the hypnotic effect of subthreshold dose of sodium pentobarbital ; ; Note: a indicates that there is a significant difference compared with the blank control group, P<0.01.

[0067] Compared with the blank control group, the sleeping rate of mice treated with the active peptide complexes of Examples 1 to 7 increased, and the difference was statistically significant (P<0.05). There was no statistically significant difference in the sleeping rate of mice treated with the active peptide complexes of Examples 1 to 5 (P>0.05).

[0068] (4) Effect of the active peptide complex on the sleep latency of sodium barbiturate The sleep latency time was statistically processed using the one-way analysis of variance method. The results are shown in Table 4: Table 4 Statistical results of the active peptide complex on the sleep latency time of sodium barbital ; ; Note: a indicates that there is a significant difference compared with the blank control group, P<0.01; b indicates that there is a significant difference compared with the fifth group of Example, P<0.01; c indicates that there is a significant difference compared with the seventh group of Example, P<0.01.

[0069] Compared with the blank control group, the sleep latency of mice treated with the active peptide complexes of Examples 1 to 5 was shortened, and the difference was statistically significant (P<0.05).

[0070] The sleep latency time of mice tested in Example 5 without theanine was significantly higher than that of mice tested in the active peptide complexes of Examples 1 to 4, but was significantly shorter than that of the blank control group, indicating that theanine has the effect of improving sleep quality.

[0071] The sleep latency of mice treated with the composition without theanine and collagen cyclic dipeptide in Example 6 increased compared with the sleep time of mice in the blank control group, but there was no significant difference between the blank control group and the composition, indicating that theanine and collagen cyclic dipeptide played the main role in the active peptide complex.

[0072] The sleep latency time of mice tested with the composition without collagen cyclic dipeptide in Example 7 was significantly higher than that of mice tested with the active peptide complexes of Examples 1 to 4, but was significantly shortened compared with the blank control group, indicating that collagen cyclic dipeptide has the effect of improving sleep quality.

[0073] (5) Direct sleep effect After the active peptide complexes in Examples 1 to 7 were administered, the animals did not experience sleep, and the number of animals that fell asleep and the sleep time were both 0, indicating that the combined use of the raw materials of the active peptide complexes had no direct sleep effect.

[0074] Therefore, the present invention provides an active peptide complex for improving sleep quality, a preparation method and an application thereof, wherein theanine is used as a surfactant and a stabilizer in combination with active peptides such as bovine milk-derived bioactive peptides that can improve sleep quality. On the one hand, an active peptide complex for improving sleep quality is provided, and on the other hand, the amount of additives in bovine milk-derived bioactive peptide products for improving sleep quality can be reduced.

[0075] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solution of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of the present invention.

Claims

1. An active peptide complex for improving sleep quality, characterized in that: Includes the following ingredients: collagen cyclic dipeptide, bovine milk-derived sleep-promoting peptide, tea theanine, and gamma-aminobutyric acid.

2. The active peptide complex for improving sleep quality according to claim 1, characterized in that: The weight ratio of each component is: collagen cyclic dipeptide 22.73%~37.04%, bovine milk-derived sleep-promoting peptide 22.73%~37.04%, tea theanine 28.57%~45.45%, and γ-aminobutyric acid 6.25%~14.29%.

3. The active peptide complex for improving sleep quality according to claim 2, characterized in that: The weight ratio of each component is: collagen cyclic dipeptide 31.25%, bovine milk-derived sleep-promoting peptide 31.25%, tea theanine 31.25%, and γ-aminobutyric acid 6.25%.

4. The active peptide complex for improving sleep quality according to claim 2, characterized in that: The weight ratio of each component is: collagen cyclic dipeptide 22.73%, bovine milk-derived sleep-promoting peptide 22.73%, tea theanine 45.45%, and γ-aminobutyric acid 9.09%.

5. The active peptide complex for improving sleep quality according to claim 2, characterized in that: The weight ratio of each component is: collagen cyclic dipeptide 37.04%, bovine milk-derived sleep-promoting peptide 18.52%, tea theanine 37.04%, and γ-aminobutyric acid 7.41%.

6. The active peptide complex for improving sleep quality according to claim 2, characterized in that: The weight ratio of each component is: collagen cyclic dipeptide 28.57%, bovine milk-derived sleep-promoting peptide 28.57%, tea theanine 28.57%, and γ-aminobutyric acid 14.29%.

7. A method for preparing an active peptide complex for improving sleep quality as claimed in any one of claims 1 to 6, characterized in that: The steps include: (1) Weigh the components of the active peptide complex according to the ratio and set aside; (2) Add collagen cyclic dipeptide and bovine milk-derived sleep-promoting peptide to tea theanine, stir evenly to obtain a mixed solution; (3) Add γ-aminobutyric acid to the mixed solution in step (2), stir evenly, then slowly add the solvent and stir until all the raw materials are fully dissolved.

8. The method for preparing an active peptide complex for improving sleep quality according to claim 7, characterized in that: The solvent is water, and the volume of the solvent is measured according to the final volume to be prepared.

9. Use of the active peptide complex for improving sleep quality as claimed in any one of claims 1 to 6 in the preparation of protein drinks and / or medicines.

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