Cell slide making and dyeing all-in-one machine

Abstract

The invention relates to the technical field of biological experiment equipment, in particular to a cell slide-making and dyeing all-in-one machine which comprises a machine table, a centrifugal slide-making mechanism and a dyeing mechanism, the centrifugal slide-making mechanism and the dyeing mechanism are arranged on the machine table, and the centrifugal slide-making mechanism comprises a centrifugal disc driven by a driving device to rotate and a centrifugal test tube rotatably arranged on the centrifugal disc; the centrifugal test tube rotates around a rotating axis X in the first rotating direction when the centrifugal disc rotates, and a separating and fixing device is arranged between the centrifugal test tube and the centrifugal disc and can apply force in the first rotating direction to the bottom of the centrifugal test tube when centrifugal force borne by the centrifugal test tube is finished. The separating and fixing device is arranged between the centrifugal test tube and the centrifugal disc and can apply force in the first rotating direction to the bottom of the centrifugal test tube when centrifugal force borne by the centrifugal test tube is finished, so that upper-layer liquid in the centrifugal test tube can be directly poured out from a tube opening of the centrifugal test tube, and waste liquid can be conveniently discharged; and other parts cannot be polluted.

Description

Technical Field

[0001] The invention relates to the technical field of biological experimental equipment, in particular to an all-in-one cell preparation and staining machine. Background Art

[0002] With the continuous improvement of pathology technology, the second-generation liquid-based cell preparation method represented by sedimentation liquid-based cell preparation technology has been more and more widely used in the field of pathology diagnosis, especially in the preparation of cytopathology samples. This type of technology improves the diagnostic efficiency and accuracy by improving the traditional sample processing process.

[0003] However, in the prior art, such as the invention patent disclosed in the announcement number CN108931414B, a cell preparation and staining integrated machine is provided at the bottom of the centrifugal cylinder, and the opening is closed by a positioning column to achieve the uniform release of waste liquid, thereby reducing the downtime during the operation of the equipment. Specifically, this design enables the positioning column to be fixed on the positioning plate, and the positioning plate is installed on the centrifugal shaft; the centrifugal cylinder is placed on a centrifugal turntable that can slide along the centrifugal shaft. When the waste liquid needs to be discharged, the positioning column can be separated from the centrifugal cylinder by moving the centrifugal turntable away from the positioning plate, and then the bottom opening is opened for the waste liquid to be discharged. This solution effectively simplifies the waste liquid treatment process, but it also brings some new disadvantages: first, the positioning column used to block the waste liquid outlet of the centrifugal cylinder will directly contact the waste liquid, which not only increases the difficulty of cleaning, but also may become one of the factors affecting the working efficiency of the entire system. Secondly, if the positioning column cannot be thoroughly cleaned, there is a risk of contaminating the subsequent cell samples to be processed, which poses a potential threat to ensuring the accuracy and reliability of the experimental results.

[0004] Therefore, in order to solve the above problems, the present invention provides a cell preparation and staining all-in-one machine. Summary of the invention

[0005] The object of the present invention is to provide a cell slide preparation and staining integrated machine to solve the problems raised in the above-mentioned background technology.

[0006] The present invention is achieved through the following technical solutions: A cell slide preparation and staining machine comprises a machine platform and a centrifugal slide preparation mechanism and a staining mechanism arranged on the machine platform, wherein the centrifugal slide preparation mechanism comprises a centrifugal disk driven to rotate by a driving device and a centrifugal test tube rotatably arranged on the centrifugal disk, wherein the centrifugal test tube rotates along a first rotation direction around a rotation axis X when the centrifugal disk rotates, and a separation device is arranged between the centrifugal test tube and the centrifugal disk, wherein the separation device is constructed to be able to apply a force along the first rotation direction to the bottom of the centrifugal test tube when the centrifugal force applied to the centrifugal test tube ends.

[0007] Optionally, the centrifugal film-making mechanism also includes a sleeve, which is rotatably arranged on the centrifugal disk around a rotation axis X, a first slot is arranged at one end of the sleeve, and a second slot connected to the first slot is arranged at the other end of the sleeve, the centrifugal test tube is a tubular structure with openings at both ends and is pluggable in the first slot, and a slide capable of covering the tube mouth of the centrifugal test tube is pluggable in the second slot.

[0008] Optionally, a clearance groove is provided at a communication position between the first slot and the second slot, and the clearance groove shares an opening with the second slot.

[0009] Optionally, the centrifugal film-making mechanism also includes a U-shaped bracket, the bottom of which is fixed to the centrifugal disk, columnar connecting shafts are provided on opposite sides of the sleeve, and inclined connecting grooves are provided on the two inner side walls of the bracket, the connecting grooves form an opening on the end surface of the bracket, and the sleeve is movably connected to the connecting groove via the connecting shaft.

[0010] Optionally, the de-fixing device includes a traction frame, which is rotatably arranged on the bracket around a rotation axis Y and a first torsion spring is arranged at the rotation connection position of the two. The free end of the traction frame can be magnetically fixed or clamped to the sleeve after the centrifuge test tube is rotated by a predetermined angle along the first rotation direction.

[0011] Optionally, a permanent magnet is fixedly provided at the free end of the traction frame, an iron piece is fixedly provided on the outer wall of the sleeve, and the permanent magnet is magnetically fixed to the iron piece after the centrifugal test tube rotates a predetermined angle along the first rotation direction.

[0012] Optionally, a clip-on frame is fixedly provided on the side wall of the sleeve, and the clip-on frame includes a fixing portion and a folding portion, the first end of the fixing portion is fixedly connected to the outer wall of the sleeve, the folding portion is rotatably connected to the second end of the fixing portion via a rotating shaft, the second end of the fixing portion is provided with a protrusion for limiting the rotation angle of the folding portion, and a second torsion spring is provided at the rotational connection position between the fixing portion and the folding portion.

[0013] Optionally, the centrifugal film making mechanism further comprises a waste liquid tank, which is mounted on the centrifugal disk, and the waste liquid in the centrifugal test tube can be discharged into the waste liquid tank after the centrifugal test tube is rotated by a predetermined angle along the first rotation direction.

[0014] Optionally, the dyeing mechanism includes a rotating cylinder, a lifting cylinder, a connecting frame, a liquid suction device and a dye storage tank, the cylinder body of the rotating cylinder is fixedly mounted on the machine platform, the cylinder body of the lifting cylinder is fixed to the output end of the rotating cylinder, the liquid suction device is fixedly connected to the output end of the lifting cylinder through the connecting frame, and the dye storage tank and the centrifugal test tube are arranged on the rotating path of the liquid suction device.

[0015] Optionally, the liquid suction device includes a shell, a rubber bag, a suction cylinder and a liquid delivery pipe, the shell is fixed on the connecting frame, the rubber bag is arranged in the shell, the suction cylinder is installed on the top of the shell, the output end of the suction cylinder extends into the inner wall of the shell and is fixedly connected to a pressure plate, and the liquid delivery pipe is arranged at the bottom of the shell and is connected to the rubber bag.

[0016] Compared with the prior art, the present invention provides a cell slide preparation and staining all-in-one machine, which has the following beneficial effects: 1. The present invention provides a separation device between the centrifugal test tube and the centrifugal disk. The separation device can apply a force along the first rotation direction to the bottom of the centrifugal test tube when the centrifugal force applied to the centrifugal test tube ends, so that the upper layer of liquid in the centrifugal test tube can be directly poured out from the tube mouth of the centrifugal test tube, which facilitates the discharge of waste liquid and does not pollute other components. 2. The present invention provides a traction frame and a first torsion spring. When the centrifugal disc rotates, the traction frame rotates in the second rotation direction and meets the sleeve rotating in the first rotation direction. The two are fixed together by magnetic attraction or clamping. When the centrifugal disc stops rotating, the traction frame resets under the rebound force of the first torsion spring, driving the sleeve to continue rotating in the first rotation direction, thereby achieving the purpose of automatically discharging the waste liquid in the centrifugal test tube; 3. The present invention provides a clamping frame used in conjunction with the traction frame. When the centrifugal disk rotates, the traction frame is clamped together with the clamping frame under the action of centrifugal force. When the centrifugal disk stops rotating, the traction frame drives the sleeve to continue rotating along the first rotation direction through the clamping cooperation between the clamping frame and the traction frame, thereby achieving the purpose of automatically discharging the waste liquid in the centrifugal test tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is a structural schematic diagram of the centrifugal tableting mechanism of the present invention; Figure 3 It is a cross-sectional structural schematic diagram of the centrifugal tableting mechanism of the present invention; Figure 4 It is a schematic diagram of the connection structure between the centrifugal test tube and the bracket of the present invention; Figure 5 For the present invention Figure 4 A schematic diagram of the enlarged structure at point A; Figure 6 It is a schematic diagram of the separation structure of the glass slide and the sleeve of the present invention; Figure 7 For the present invention Figure 4 Schematic diagram of the cross-section structure; Figure 8 It is a schematic structural diagram of the centrifugal test tube and the traction frame of the present invention in three states: before the centrifugal disk rotates, during the rotation, and when the rotation stops; Fig. 9 It is a partial structural schematic diagram of the dyeing mechanism of the present invention; Fig.10 It is a schematic structural diagram of the liquid aspiration device of the present invention; Fig.11 It is a structural schematic diagram of the clamping cooperation between the traction frame and the clamping frame of the present invention; Fig.12 It is a structural schematic diagram of the card-joint frame of the present invention.

[0018] In the figure: 1, machine; 2, driving device; 3, centrifugal disc; 4, centrifugal test tube; 5, sleeve; 6, first slot; 7, second slot; 8, slide; 9, give way slot; 10, bracket; 11, connecting shaft; 12, connecting slot; 13, traction frame; 14, first torsion spring; 15, permanent magnet; 16, iron piece; 17, fixing part; 18, folding part; 19, rotating shaft; 20, protrusion; 21, second torsion spring; 22, waste liquid tank; 23, lifting rod; 24, inserting rod; 25, sliding block; 26, sliding groove; 260, insertion end; 261, limiting end; 27, rotating cylinder; 28, lifting cylinder; 29, connecting frame; 30, dye storage tank; 31, liquid suction device; 310, shell; 311, rubber bag; 312, suction cylinder; 313, liquid delivery pipe; 314, pressure plate. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] Example: See Figures 1 to 12According to an embodiment of the present invention, a cell preparation and staining machine is provided, which includes a machine platform 1 and a centrifugal preparation mechanism and a staining mechanism arranged on the machine platform 1. The centrifugal preparation mechanism includes a centrifugal disk 3 driven to rotate by a driving device 2 and a centrifugal test tube 4 rotatably arranged on the centrifugal disk 3. Specifically, the driving device 2 is a servo motor, and the housing 310 of the servo motor is fixed on the machine platform 1. The output shaft of the servo motor and the centrifugal disk 3 are coaxially fixed to the centrifugal disk 3 through a flange. When the centrifugal disk 3 rotates, the centrifugal test tube 4 rotates around a rotation axis X along a first rotation direction. A separation device is arranged between the centrifugal test tube 4 and the centrifugal disk 3. The separation device is constructed to be able to apply a force along the first rotation direction to the bottom of the centrifugal test tube 4 when the centrifugal force applied to the centrifugal test tube 4 ends.

[0021] In the cell preparation and staining integrated machine with the above structure, when the centrifugal disc 3 is in a stationary state, the centrifugal test tube 4 is in a vertical state with the opening facing upward. At this time, a preservation solution containing a cell sample can be added to the centrifugal test tube 4. Then, the centrifugal disc 3 is controlled to rotate, and under the action of centrifugal force, the centrifugal test tube 4 rotates along the first rotation direction around the rotation axis X. Since the density of the cells is greater than the density of the preservation solution, the cells will gather at the bottom of the centrifugal test tube 4 to achieve centrifugal sedimentation. After the centrifugal sedimentation is completed, the centrifugal disc 3 stops rotating, and the solid separation device applies a force along the first rotation direction to the bottom of the centrifugal test tube 4, so that the centrifugal test tube 4 maintains the current tilted state or continues to rotate along the first rotation direction. In this way, the upper layer of liquid (i.e., waste liquid) in the centrifugal test tube 4 will be directly poured out from the mouth of the centrifugal test tube 4, which is convenient for the discharge of waste liquid and will not contaminate other components.

[0022] Combination Figures 4 to 7 In this exemplary embodiment, the centrifugal slice making mechanism further includes a sleeve 5, which is rotatably arranged on the centrifugal disk 3 around the rotation axis X, a first slot 6 is arranged at one end of the sleeve 5, and a second slot 7 connected to the first slot 6 is arranged at the other end of the sleeve 5. The centrifugal test tube 4 is a tubular structure with two ends open, the first end of the centrifugal test tube 4 is pluggable and arranged in the first slot 6, the second end of the centrifugal test tube 4 is exposed outside the first slot 6, and a slide glass 8 capable of covering the tube opening of the first end of the centrifugal test tube 4 is pluggable and arranged in the second slot 7. Through this arrangement, when the centrifugal disk 3 starts to rotate, the cells in the centrifugal test tube 4 will gather toward the first end of the centrifugal test tube 4 under the action of centrifugal force, and finally be evenly distributed on the slide glass 8, so as to realize the direct centrifugal sedimentation slice making of the cells, and omit the step of transferring the cells in the centrifugal test tube 4 to the slide glass 8 in the traditional method. In addition, during the entire centrifugation process, due to the centrifugal force, the centrifugal test tube 4 will be tightly pressed against the glass slide 8, ensuring the sealing of the connection between the two, effectively preventing the leakage of the preservation solution, and further improving the safety and efficiency of the preparation process.

[0023] Combination Figure 6and Figure 7 In this exemplary embodiment, a clearance groove 9 is provided at the connecting position between the first slot 6 and the second slot 7, and the clearance groove 9 shares an opening with the second slot 7. By providing the clearance groove 9, it is possible to effectively avoid scratching between the side of the slide 8 for attaching cells and the inner wall of the second slot 7, thereby ensuring that the cells on the slide 8 are evenly distributed and remain intact. This not only protects the quality of the cell sample, but also improves the reliability and consistency of the preparation process, making the final cell smear clearer and more accurate, and facilitating subsequent microscopic observation and pathological diagnosis.

[0024] Combination Figure 4 In this exemplary embodiment, the centrifugal film making mechanism further includes a U-shaped bracket 10, the bottom of the bracket 10 is fixed to the centrifugal disc 3, and the opposite sides of the sleeve 5 are provided with a columnar connecting shaft 11. The two inner side walls of the bracket 10 are provided with an inclined connecting groove 12, and the connecting groove 12 forms an opening on the end surface of the bracket 10. The sleeve 5 is movably connected to the connecting groove 12 through the connecting shaft 11, that is, the connecting shaft 11 can rotate and slide in the connecting groove 12. Through this arrangement, when the centrifugal test tube 4 and the sleeve 5 are rotated in the first rotation direction under the action of centrifugal force, due to the inclined setting of the connecting groove 12, the connecting shaft 11 will always remain at the lower end of the connecting groove 12, which can not only ensure the smooth rotation of the centrifugal test tube 4 and the sleeve 5, but also prevent the connecting shaft 11 from being separated from the connecting groove 12, thereby ensuring the stability and safety of the entire system. In addition, since the connecting groove 12 forms an opening on the end surface of the bracket 10, this design not only facilitates the assembly between the sleeve 5 and the bracket 10, but also provides convenience for the cleaning and replacement of the sleeve 5, further improving the maintenance efficiency and operational convenience of the equipment.

[0025] Combination Figure 4 and Figure 5 In this exemplary embodiment, the separation device includes a traction frame 13, which is rotatably arranged on the bracket 10 around a rotation axis Y and a first torsion spring 14 is arranged at the rotation connection position of the two. Specifically, the rotation axis Y can be colinear with the rotation axis X, and the free end of the traction frame 13 can be fixed to the sleeve 5 by magnetic attraction or clamping after the centrifugal test tube 4 rotates a predetermined angle along the first rotation direction. Through this design, when the centrifugal disc 3 starts to rotate, the traction frame 13 overcomes the elastic force of the first torsion spring 14 under the action of centrifugal force and rotates around the rotation axis Y along the second rotation direction opposite to the first rotation direction, and finally meets the sleeve 5 rotating along the first rotation direction, and is fixed together by magnetic attraction or clamping. When the centrifugal disc 3 stops rotating, the centrifugal force disappears, and the rebound force of the first torsion spring 14 causes the traction frame 13 to rotate along the first rotation direction, thereby driving the sleeve 5 to continue to rotate along the first rotation direction, thereby achieving the purpose of automatically discharging the waste liquid in the centrifugal test tube 4.

[0026] In order to achieve that the free end of the traction frame 13 can be magnetically fixed to the sleeve 5 after the centrifugal tube 4 rotates a predetermined angle along the first rotation direction, in an exemplary embodiment, a permanent magnet 15 is fixedly provided at the free end of the traction frame 13, and an iron piece 16 is fixedly provided on the outer wall of the sleeve 5. The permanent magnet 15 is magnetically fixed to the iron piece 16 after the centrifugal tube 4 rotates a predetermined angle along the first rotation direction. Figure 8 As shown, from left to right, the three states of the centrifugal test tube 4 and the traction frame 13 are respectively shown before the centrifugal disc 3 rotates, during the rotation, and when the rotation stops. Before the centrifugal disc 3 rotates, the centrifugal test tube 4 is in a vertical state, and the free end of the traction frame 13 is in a higher position; during the rotation of the centrifugal disc 3, the centrifugal test tube 4 rotates along the first rotation direction to an almost horizontal state, at which time the permanent magnet 15 fixed at the free end of the traction frame 13 is attracted together with the iron piece 16 on the sleeve 5; after the centrifugal disc 3 rotates, the centrifugal test tube 4 is pulled by the traction frame 13 and continues to rotate along the first rotation direction until all the waste liquid in the centrifugal test tube 4 is discharged into the waste liquid tank 22. Of course, as long as it can be ensured that the free end of the traction frame 13 is magnetically fixed to the sleeve 5 after the centrifugal test tube 4 rotates along the first rotation direction by a predetermined angle, there is no special restriction on the specific magnetic fixing method between the traction frame 13 and the sleeve 5. For example, other types of magnetic materials or magnetic structures can be used to achieve this function.

[0027] Combination Fig.11 and Fig.12In order to realize that the free end of the traction frame 13 can be clamped and fixed with the sleeve 5 after the centrifugal test tube 4 rotates a predetermined angle along the first rotation direction, in another exemplary embodiment, a clamping frame is fixedly arranged on the side wall of the sleeve 5, and the clamping frame includes a fixing portion 17 and a folding portion 18. The first end of the fixing portion 17 is fixedly connected to the outer wall of the sleeve 5, and the folding portion 18 is rotatably connected to the second end of the fixing portion 17 through a rotating shaft 19. The second end of the fixing portion 17 is provided with a protrusion 20 for limiting the rotation angle of the folding portion 18, and the rotation connection position of the fixing portion 17 and the folding portion 18 is provided with a second torsion spring 21. With this design, when the centrifugal disc 3 starts to rotate, the traction frame 13 rotates along the second rotation direction opposite to the first rotation direction under the action of centrifugal force. As the traction frame 13 moves, it pushes the folding portion 18 to overcome the elastic force of the second torsion spring 21 and rotate downward around the rotating shaft 19. After the traction frame 13 passes over the folding portion 18, the folding portion 18 rotates upward under the action of the rebound force of the second torsion spring 21. When the centrifugal disc 3 stops rotating, the traction frame 13 and the sleeve 5 are engaged with each other through the engagement of the engaging frame and the traction frame 13, thereby achieving the purpose of automatically discharging the waste liquid in the centrifugal test tube 4. Of course, as long as it can be ensured that the free end of the traction frame 13 is engaged with the sleeve 5 after the centrifugal test tube 4 rotates a predetermined angle along the first rotation direction, there is no particular limitation on the specific engagement and fixing method between the traction frame 13 and the sleeve 5. For example, other types of engagement structures or mechanisms can be used to achieve this function.

[0028] Combination Figure 3In this exemplary embodiment, the centrifugal film making mechanism also includes a waste liquid tank 22, which is installed on the centrifugal disk 3. The waste liquid in the centrifugal test tube 4 can be discharged into the waste liquid tank 22 after the centrifugal test tube 4 rotates a predetermined angle along the first rotation direction, thereby facilitating the centralized treatment and collection of the waste liquid. Specifically, a plurality of brackets 10 are arranged around the center of the centrifugal disk 3, and these brackets 10 support and fix components such as the sleeve 5. The waste liquid tank 22 is arranged at the center of the centrifugal disk 3, and a hollow lifting rod 23 is fixed in the waste liquid tank 22, and the lifting rod 23 forms an opening at the bottom of the waste liquid tank 22. A plug rod 24 is vertically fixed at the center of the centrifugal disk 3, and this plug rod 24 can be inserted into the lifting rod 23 from the opening at the bottom of the lifting rod 23 to play a positioning and fixing role. A T-shaped slider 25 is provided at the bottom of each bracket 10, and a corresponding slide groove 26 is provided on the centrifugal disk 3. One end of the chute 26 close to the waste liquid tank 22 is an insertion end 260, and the end opposite to the insertion end 260 is a limiting end 261. In specific implementation, the T-shaped slider 25 at the bottom of the bracket 10 is inserted from the insertion end 260 of the chute 26, and then slides to the limiting end 261, so that the vertical position of the bracket 10 on the centrifugal disk 3 can be limited to prevent it from moving during the centrifugal process. Then, the waste liquid tank 22 is placed in the space surrounded by multiple brackets 10, and the movement of the waste liquid tank 22 in the radial direction of the centrifugal disk 3 is limited by the insertion rod 24. At the same time, the waste liquid tank 22 also limits the movement of the bracket 10 along the radial direction of the centrifugal disk 3, thereby ensuring the stability and reliability of the entire structure. Through this design, the installation of the bracket 10 and the waste liquid tank 22 on the centrifugal disk 3 can be easily completed. When disassembling, it is only necessary to remove the waste liquid tank 22 first, and then move the bracket 10 along the center position of the centrifugal disk 3 to easily remove it. This design simplifies the assembly and maintenance process of the centrifugal film making mechanism.

[0029] Combination Figure 1 and Fig. 9In order to dye the cells, in this exemplary embodiment, the dyeing mechanism includes a rotating cylinder 27, a lifting cylinder 28, a connecting frame 29, a liquid suction device 31 and a dye storage tank 30. The cylinder body of the rotating cylinder 27 is fixedly mounted on the machine 1, the cylinder body of the lifting cylinder 28 is fixed to the output end of the rotating cylinder 27, the liquid suction device 31 is fixedly connected to the output end of the lifting cylinder 28 through the connecting frame 29, and the dye storage tank 30 and the centrifugal test tube 4 are arranged on the rotation path of the liquid suction device 31. After the cells are centrifugally sliced ​​on the centrifugal slice making mechanism, they can be stained by the dyeing mechanism. During staining, the rotating cylinder 27 controls the position of the liquid suction device 31 in the horizontal direction, so that the liquid suction device 31 can move back and forth between the dye storage tank 30 and the centrifugal test tube 4. The lifting cylinder 28 can control the vertical position of the liquid suction device 31, reduce the vertical distance between the liquid suction device 31 and the dye storage tank 30 and the centrifugal test tube 4, so as to ensure that the liquid suction device 31 can accurately suck the dye in the dye storage tank 30 and accurately drop the sucked dye into the centrifugal test tube 4. This design not only improves the accuracy and efficiency of the dyeing process, but also reduces the error of human operation, ensuring the consistency and uniformity of dyeing.

[0030] Combination Fig.10 In order to absorb and release the dye, in this exemplary embodiment, the liquid suction device 31 includes a housing 310, a rubber bag 311, a suction cylinder 312 and a liquid delivery pipe 313. The housing 310 is fixed on the connecting frame 29, the rubber bag 311 is arranged in the housing 310, the suction cylinder 312 is installed on the top of the housing 310, the output end of the suction cylinder 312 extends into the inner wall of the housing 310 and is fixedly connected with a pressure plate 314, and the liquid delivery pipe 313 is arranged at the bottom of the housing 310 and communicates with the rubber bag 311. With the above arrangement, when the liquid suction device 31 moves to the top of the dye storage tank 30, the suction cylinder 312 first drives the pressure plate 314 to move downward to compress the rubber bag 311. Then, the lifting cylinder 28 controls the liquid suction device 31 to move downward, so that the liquid delivery pipe 313 is inserted into the dye storage tank 30. Then, the suction cylinder 312 drives the pressure plate 314 to move upward, and negative pressure is generated in the rubber bag 311. At this time, the dye is sucked into the rubber bag 311 by the liquid delivery tube 313, and the dye is absorbed. Finally, the liquid suction device 31 is moved to the top of the centrifugal test tube 4, and the lifting cylinder 28 drives the pressure plate 314 to move downward, and the dye in the rubber bag 311 is squeezed outward. The squeezed dye falls into the centrifugal test tube 4, and the cells in the centrifugal test tube 4 are dyed. This design ensures the accurate absorption and release of the dye, and improves the accuracy and consistency of the dyeing process. In addition, by controlling the actions of the suction cylinder 312 and the lifting cylinder 28, automatic operation can be achieved, reducing human intervention, and further improving the dyeing efficiency and quality.

[0031] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell slide preparation and staining machine, comprising a machine platform (1) and a centrifugal slide preparation mechanism and a staining mechanism arranged on the machine platform (1), characterized in that: The centrifugal film making mechanism comprises a centrifugal disc (3) driven to rotate by a driving device (2) and a centrifugal test tube (4) rotatably arranged on the centrifugal disc (3); the centrifugal test tube (4) rotates around a rotation axis X along a first rotation direction when the centrifugal disc (3) rotates; a separation device is arranged between the centrifugal test tube (4) and the centrifugal disc (3); the separation device is constructed to be able to apply a force along the first rotation direction to the bottom of the centrifugal test tube (4) when the centrifugal force applied to the centrifugal test tube (4) ends.

2. The integrated cell preparation and staining machine according to claim 1, characterized in that: The centrifugal film making mechanism further comprises a sleeve (5), the sleeve (5) being rotatably arranged on the centrifugal disc (3) around a rotation axis X, one end of the sleeve (5) being provided with a first slot (6), the other end of the sleeve (5) being provided with a second slot (7) connected to the first slot (6), the centrifugal test tube (4) being a tube structure with openings at both ends and being pluggable and arranged in the first slot (6), and a glass slide (8) capable of covering the tube opening of the centrifugal test tube (4) being pluggable and arranged in the second slot (7).

3. The integrated cell preparation and staining machine according to claim 2, characterized in that: A clearance groove (9) is provided at the communicating position between the first slot (6) and the second slot (7), and the clearance groove (9) shares an opening with the second slot (7).

4. The integrated cell preparation and staining machine according to claim 2 or 3, characterized in that: The centrifugal tablet making mechanism further comprises a U-shaped bracket (10), the bottom of the bracket (10) being fixed to the centrifugal disc (3), columnar connecting shafts (11) being arranged on opposite sides of the sleeve (5), and obliquely arranged connecting grooves (12) being provided on the two inner side walls of the bracket (10), the connecting grooves (12) forming an opening on the end surface of the bracket (10), and the sleeve (5) being movably connected to the connecting grooves (12) via the connecting shafts (11).

5. The integrated cell preparation and staining machine according to claim 4, characterized in that: The de-fixing device comprises a traction frame (13), the traction frame (13) being rotatably arranged on the bracket (10) around a rotation axis Y, and a first torsion spring (14) being arranged at a rotational connection position between the two, and the free end of the traction frame (13) being able to be magnetically fixed or clamped to the sleeve (5) after the centrifugal test tube (4) is rotated by a predetermined angle along a first rotation direction.

6. The integrated cell preparation and staining machine according to claim 5, characterized in that: A permanent magnet (15) is fixedly provided at the free end of the traction frame (13), an iron piece (16) is fixedly provided on the outer wall of the sleeve (5), and the permanent magnet (15) is magnetically fixed to the iron piece (16) after the centrifugal test tube (4) rotates by a predetermined angle in the first rotation direction.

7. The integrated cell preparation and staining machine according to claim 5, characterized in that: A clamping frame is fixedly arranged on the side wall of the sleeve (5), and the clamping frame comprises a fixing portion (17) and a folding portion (18). A first end of the fixing portion (17) is fixedly connected to the outer wall of the sleeve (5), and the folding portion (18) is rotatably connected to a second end of the fixing portion (17) via a rotating shaft (19). A protrusion (20) for limiting the rotation angle of the folding portion (18) is arranged at the second end of the fixing portion (17), and a second torsion spring (21) is arranged at a rotation connection position between the fixing portion (17) and the folding portion (18).

8. The integrated cell preparation and staining machine according to any one of claims 5 to 7, characterized in that: The centrifugal film making mechanism further comprises a waste liquid tank (22), wherein the waste liquid tank (22) is mounted on the centrifugal disk (3), and waste liquid in the centrifugal test tube (4) can be discharged into the waste liquid tank (22) after the centrifugal test tube (4) is rotated by a predetermined angle along a first rotation direction.

9. The integrated cell preparation and staining machine according to any one of claims 5 to 7, characterized in that: The dyeing mechanism comprises a rotating cylinder (27), a lifting cylinder (28), a connecting frame (29), a liquid suction device (31) and a dye storage tank (30); the cylinder body of the rotating cylinder (27) is fixedly mounted on the machine platform (1); the cylinder body of the lifting cylinder (28) is fixed to the output end of the rotating cylinder (27); the liquid suction device (31) is fixedly connected to the output end of the lifting cylinder (28) via the connecting frame (29); and the dye storage tank (30) and the centrifugal test tube (4) are arranged on a rotating path of the liquid suction device (31).

10. The integrated cell preparation and staining machine according to claim 9, characterized in that: The liquid suction device (31) comprises a housing (310), a rubber bag (311), a suction cylinder (312) and a liquid delivery pipe (313); the housing (310) is fixed to the connecting frame (29); the rubber bag (311) is arranged in the housing (310); the suction cylinder (312) is installed on the top of the housing (310); the output end of the suction cylinder (312) extends into the inner wall of the housing (310) and is fixedly connected to a pressure plate (314); and the liquid delivery pipe (313) is arranged at the bottom of the housing (310) and is in communication with the rubber bag (311).

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