Microbial fluorescence detection system and detection method

Abstract

The invention provides a microorganism fluorescence detection system and a detection method, and relates to the technical field of microorganism gene detection.The detection system comprises a machine body, a detection hole, a driving plate, a mounting plate, a containing bottle, a guide pipe and an automatic liquid dropping mechanism, the detection hole is formed in one side of the machine body, the driving plate is mounted in the detection hole, the mounting plate is mounted on the machine body, and the containing bottle is arranged in the machine body; a plurality of containing bottles are mounted on one side of the mounting plate, and one end of each guide pipe is communicated with the corresponding containing bottle. According to the invention, the pipette can be automatically controlled to dropwise add to-be-detected liquid into a plurality of detection cavities of the detection vessel, the automatic dropwise adding process enables the experimental process to be more coherent and systematized, the operation steps and time waste are reduced, the detection efficiency is improved, the error caused by manual operation is reduced, and the reliability of the experimental result is improved; and the conveying belt can seal and shield the detection cavity in the liquid dropping process, so that the detection liquid can be prevented from sputtering in the dropping process, the sample loss influence can be facilitated, and the experimental environment pollution can be avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial gene detection, and in particular to a microbial fluorescence detection system and a detection method. Background Art

[0002] Food safety is regarded as a global public health issue, and foodborne pathogens are an important aspect of food safety. Vegetables, fruits and vegetables are nutritious foods. Due to the high water content of vegetables, they are easily infected by pathogenic microorganisms during processing and transportation, thus causing pathogenic diseases and endangering human health. Four foodborne pathogens, Escherichia coli, Salmonella, Listeria monocytogenes, Staphylococcus aureus, as well as norovirus and rotavirus, are common pathogenic microorganisms carried by vegetables.

[0003] Vegetables are essential foods in daily life. The contamination of vegetables with pathogenic microorganisms is closely related to the health of consumers. Therefore, pathogenic microorganism testing of vegetables, especially raw vegetables, is crucial to protecting the health of the people.

[0004] Fluorescence imaging is an indispensable tool in biological research, especially in cell research. By staining cells or organelles with various fluorophores and magnifying and imaging them under a microscope, a large number of color-coded processes can be quantitatively characterized, enabling the study of cellular gene expression based on chromosome dynamics. Fluorophores have greatly improved cell research by introducing high quantum yield fluorescent dyes and multiple staining methods to the field. This allows researchers to simultaneously study several organelles and their interactions in the same FOV (field of view) with high contrast. Microbial fluorescence detection system is a technology used to detect and quantify microorganisms, mainly used to monitor and detect microbial contamination in food, such as bacteria, fungi, etc.

[0005] The invention with publication number CN110823854B discloses a fluorescence spectrum detection system for microorganisms, and the key points of its technical solution are: including a laser light source emitting laser to the microorganism sample to be tested carried on a light-transmitting platform to excite the fluorescent dye to emit fluorescence; the light-transmitting platform is arranged on the focal plane of a microscopic lens group; the microscopic lens group amplifies the fluorescent light transmitted by the light-transmitting platform to form parallel light; the filter element filters the parallel light; the first focusing lens group focuses the parallel light to form a fluorescent image, and divides it into a plurality of image units by an integral field of view unit, and disperses the plurality of image units through a spectrometer; the detector detects the obtained spectral distribution information. The present application does not need to scan the fluorescent light in the field of view of the microscopic lens group one by one, realizes the synchronous dispersion of the fluorescent light in the field of view of the microscopic lens group, improves the observation efficiency of the fluorescence detection of the microbial sample, is conducive to capturing the instantaneous changes of cells or organelles in the microorganism, and improves the accuracy of microbial research.

[0006] With respect to the above-mentioned related contents, the following technical defects were found: When using the microbial fluorescence detection system in the prior art, personnel need to manually use a pipette to drip the test liquid into multiple test cavities on a test dish, then put the test dish containing the test liquid into the instrument, and finally start the instrument to perform the microbial fluorescence detection operation. However, the manual dripping method will cause the following problems: 1. The personnel need to manually control the pipette to drip the liquid to be tested into the test dish. Manually controlling the pipette to drip the liquid to be tested consumes the personnel's time and energy, increases the complexity of the operation, and affects the efficiency and accuracy of the test; 2. In the process of adding the test liquid to the test dish, the test liquid is prone to sputtering, which will not only cause sample loss and affect the reliability of the experimental results, but also easily cause experimental environmental pollution and increase the workload of cleaning and maintenance. Summary of the invention

[0007] The purpose of the present invention is to solve the shortcomings of the prior art and to propose a microbial fluorescence detection system.

[0008] In order to achieve the above-mentioned purpose, the present invention adopts the following technical scheme: a microorganism fluorescence detection system, including a body, a detection hole, a drive plate, a mounting plate, a holding bottle, a catheter and an automatic dripping mechanism, the detection hole is arranged on one side of the body, the drive plate is installed inside the detection hole, the mounting plate is installed on the body, a plurality of holding bottles are installed on one side of the mounting plate, one end of the catheter is connected with the holding bottle, the automatic dripping mechanism is located on one side of the body, the automatic dripping mechanism includes a support plate, a detection dish and an automatic pipette gun, a plurality of detection cavities are opened on the upper surface of the detection dish, the detection cavities are evenly distributed on the upper surface of the detection dish, the lower surface of the detection dish and the upper surface of the drive plate are located in the same plane, one side of the support plate is fixedly connected to the body, and one side of the support plate is fixedly connected with A servo motor, a sliding hole is provided on the upper surface of the support plate, a slider is slidably connected to the inner wall of the sliding hole, a screw rod is threaded through the side surface of the slider, the output end of the servo motor is fixedly connected to one end of the screw rod, the upper surface of the slider is fixedly connected to a connecting plate, both sides of the connecting plate are fixedly connected to a fixing seat, the inner wall of the fixing seat is rotatably connected to a transmission wheel, one side of one of the fixing seats is fixedly connected to a motor seat, the inner wall of the motor seat is fixedly connected to a driving motor, the output end of the driving motor is fixedly connected to one end of one of the transmission wheels, the arc surfaces of the two transmission wheels are transmission-connected to a conveyor belt, the upper surface of the detection dish is in contact with the conveyor belt, a circular hole is provided on the surface of the conveyor belt, a cylinder is fixedly connected to the inner wall of the circular hole, and the automatic pipette gun is located inside the cylinder.

[0009] Preferably, a positioning plate is fixedly connected to the outer wall of the cylinder, a positioning rod is slidably penetrated through the side of the positioning plate, both ends of the positioning rod are fixedly connected to fixing plates, and the two fixing plates are respectively fixedly connected to the sides of the two fixing seats away from each other.

[0010] Preferably, a plurality of rectangular blocks are fixedly connected to the arc surface of the automatic pipette gun, a plurality of rectangular holes are opened at the upper end of the cylinder, and the rectangular blocks fit with the inner walls of the rectangular holes.

[0011] Preferably, a guide block is fixedly connected to a side of the detection dish away from the driving plate, and the guide block is configured as a wedge-shaped structure.

[0012] Preferably, an automatic conveying mechanism is provided on the side of the body away from the supporting plate, and the automatic conveying mechanism comprises a welding plate and a carrying plate, one side of the welding plate is fixedly connected to the body, and the side of the welding plate away from the body is rotatably connected with a driving reel and a driven reel, and the welding plate is fixedly connected to a limiting plate on the side away from the body, and one side of the limiting plate is fixedly connected to a motor, the output end of the motor is fixedly connected to the driving reel, and the arc surface of the driving reel and the driven reel are connected with a belt for transmission, and a threaded rod is rotatably penetrated by the side of the welding plate, one end of the threaded rod is fixedly connected to the driven reel, and the arc surface of the threaded rod is threadedly connected with an ear plate, and the upper surface of the ear plate is fixedly connected with a conveying frame, and the cross-section of the conveying frame is "C" shaped, and one side of the carrying plate is in contact with the driving plate, and the upper surface of the carrying plate and the upper surface of the driving plate are located in the same plane, and the lower surface of the carrying plate is fixedly connected with an auxiliary plate, and one side of the auxiliary plate is fixedly connected to a side of the support plate close to the driving plate.

[0013] Preferably, a guide rod is slidably provided on one side of the conveying frame, and one end of the short arm end of the guide rod is fixedly connected to the welding plate.

[0014] Preferably, a first limiting hole is opened on the upper surface of the supporting plate, and a second limiting hole is opened on the upper surface of the driving plate, the first limiting hole is communicated with the second limiting hole, the inner wall of the first limiting hole is slidably connected to a limiting block, and the limiting block is fixedly connected to the lower surface of the detection dish.

[0015] Preferably, a sealing mechanism is provided on one side of the body close to the detection hole, and the sealing mechanism includes a rotating seat, one side of the rotating seat is fixedly connected to the body, a rotating shaft is rotatably penetrated through the inner wall of the rotating seat, a connecting plate is fixedly connected to the arc surface of the rotating shaft, a sealing plate is fixedly connected to one side of the connecting plate, an auxiliary hole is provided on the side of the sealing plate, an arc hole is provided on one side of the rotating seat, the center of the arc hole is coaxial with the axis of rotation of the rotating shaft, a fixing rod is slidably connected to the inner wall of the arc hole, one end of the fixing rod is fixedly connected to one side of the connecting plate, and an adjustment ring is threadedly connected to the arc surface of the fixing rod.

[0016] Preferably, a rubber pad is fixedly connected to one side of the adjusting ring close to the rotating seat, and the diameter of the rubber pad is larger than the diameter of the adjusting ring.

[0017] Preferably, the arc surface of the rotating shaft is sleeved with a coil spring, and two ends of the coil spring are respectively fixedly connected to the connecting plate and the rotating seat.

[0018] The present invention also discloses a microbial fluorescence detection method using the microbial fluorescence detection system for continued detection, comprising the steps of: 1) Use the automatic pipette to transfer 50 μL of the pre-test sample into each test cavity, and then place the test dish into the microbial fluorescence test; 2) Select the test conditions and obtain the test results in real time. The test results can be obtained within 27 minutes.

[0019] Compared with the prior art, the advantages and positive effects of the present invention are: 1. In the present invention, two effects are achieved by setting up an automatic pipetting mechanism: first, the pipette gun can be automatically controlled to drip the liquid to be tested into multiple detection cavities of the detection dish. The automatic dripping process makes the experimental process more coherent and systematic, reduces the operation steps and time waste, improves the detection efficiency, and reduces the errors caused by human operation, thereby improving the reliability of the experimental results; second, during the dripping process, the conveyor belt can seal and shield the detection cavity, thereby avoiding the sputtering of the detection liquid during the dripping process, thereby facilitating the loss of samples and avoiding pollution of the experimental environment.

[0020] 2. In the present invention, by setting up an automatic conveying mechanism, the detection dish can be automatically conveyed from the driving plate to the automatic dripping mechanism, and after the dripping operation is completed, the detection dish can be automatically conveyed from the automatic dripping mechanism to the driving plate, which further improves the degree of automation of the microbial fluorescence detection system and further improves the detection efficiency.

[0021] 3. In the present invention, by providing a sealing mechanism, after the driving plate drives the detection dish to move into the body, personnel can conveniently adjust the sealing plate to block the detection hole, thereby sealing the detection hole, thereby further improving the practicality of the microbial fluorescence detection system.

[0022] 4. The test results are completed in less than half an hour, shortening the product release time by two to five days. Therefore, storage space and costs can be reduced, inventory turnover is faster, and there is greater flexibility and responsiveness to meet customer needs. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 A schematic diagram of the three-dimensional structure of a microbial fluorescence detection system proposed by the present invention; Figure 2 A schematic diagram of the structure of an automatic dripping mechanism of a microbial fluorescence detection system proposed by the present invention; Figure 3 A partial schematic diagram of an automatic dripping mechanism of a microorganism fluorescence detection system proposed by the present invention; Figure 4 A schematic diagram of the partial structural disassembly of an automatic dripping mechanism of a microbial fluorescence detection system proposed by the present invention; Figure 5 A schematic diagram of the structure of a detection dish of a microbial fluorescence detection system proposed by the present invention; Figure 6 The present invention provides a schematic structural diagram of an automatic pipette gun for a microbial fluorescence detection system; Figure 7 A structural schematic diagram of an automatic conveying mechanism of a microorganism fluorescence detection system proposed by the present invention; Figure 8 A partial structural schematic diagram of an automatic conveying mechanism of a microorganism fluorescence detection system proposed by the present invention; Fig. 9 A structural schematic diagram of a carrier plate of a microbial fluorescence detection system proposed by the present invention; Fig.10 A structural schematic diagram of a sealing mechanism of a microbial fluorescence detection system proposed by the present invention; Fig.11 The present invention provides a partial structural schematic diagram of a sealing mechanism of a microorganism fluorescence detection system.

[0024] Legend: 1. Machine body; 2. Detection hole; 3. Automatic dripping mechanism; 301. Support plate; 302. Servo motor; 303. Slide hole; 304. Screw rod; 305. Sliding block; 306. Connecting plate; 307. Fixed seat; 308. Transmission wheel; 309. Motor seat; 310. Driving motor; 311. Conveyor belt; 312. Round hole; 313. Cylinder; 314. Automatic pipette gun; 315. Positioning plate; 316. Positioning rod; 317. Fixed plate; 318. Rectangular hole; 319. Rectangular block; 320. Detection dish; 321. Detection cavity; 322. Guide block; 4. Automatic conveying mechanism; 401. Welding plate; 402 , limit plate; 403, motor; 404, active reel; 405, driven reel; 406, belt; 407, threaded rod; 408, ear plate; 409, conveyor frame; 410, guide rod; 411, bearing plate; 412, auxiliary plate; 413, first limit hole; 414, second limit hole; 415, limit block; 5, sealing mechanism; 501, rotating seat; 502, rotating shaft; 503, connecting plate; 504, sealing plate; 505, auxiliary hole; 506, coil spring; 507, arc hole; 508, fixing rod; 509, adjusting ring; 510, rubber pad; 6, driving plate; 7, mounting plate; 8, bottle holding; 9, catheter. DETAILED DESCRIPTION

[0025] In order to more clearly understand the above-mentioned purpose, features and advantages of the present invention, the present invention is further described below in conjunction with the accompanying drawings and embodiments. It should be noted that the embodiments of the present application and the features in the embodiments can be combined with each other without conflict.

[0026] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways than those described herein. Therefore, the present invention is not limited to the specific embodiments of the following disclosure.

[0027] Embodiment 1, as Figure 1 As shown, the present invention provides a microorganism fluorescence detection system, including a body 1, a detection hole 2, a drive plate 6, a mounting plate 7, a holding bottle 8, a conduit 9 and an automatic dripping mechanism 3. A driving device is installed inside the body 1 to enable the drive plate 6 to automatically enter and exit the detection hole 2. The holding bottle 8 is used to hold fluorescent dye. The conduit 9 is used to transport the fluorescent dye from the holding bottle 8 to the detection dish 320, and the transportation process is controlled by a transportation pump inside the body 1. The detection hole 2 is arranged on one side of the body 1, the drive plate 6 is installed inside the detection hole 2, the mounting plate 7 is installed on the body 1, and a plurality of holding bottles 8 are installed on one side of the mounting plate 7, and one end of the conduit 9 is connected to the holding bottle 8.

[0028] The automatic dripping mechanism 3 is located on one side of the body 1 , the side of the body 1 away from the support plate 301 is provided with an automatic conveying mechanism 4 , and the side of the body 1 close to the detection hole 2 is provided with a sealing mechanism 5 .

[0029] A central control system is also installed inside the body 1 to control the automatic dripping mechanism 3 and the automatic conveying mechanism 4. The process and principle of the central control system receiving and generating signals are similar to those of existing common control systems and are not the focus of protection of the present invention, so they will not be elaborated in detail.

[0030] The specific configuration and functions of the automatic dripping mechanism 3, the automatic conveying mechanism 4 and the sealing mechanism 5 are described in detail below.

[0031] like Figures 2 to 6 As shown, the automatic dripping mechanism 3 includes a support plate 301, a detection dish 320 and an automatic liquid transfer gun 314. The upper surface of the detection dish 320 is provided with a plurality of detection cavities 321 for holding the liquid to be detected, and the detection cavities 321 are evenly distributed on the upper surface of the detection dish 320. The lower surface of the detection dish 320 and the upper surface of the driving plate 6 are located in the same plane, which can prevent the detection dish 320 from getting stuck during the transportation process. One side of the support plate 301 is fixedly connected to the body 1, and the support plate 301 plays a supporting role.

[0032] A servo motor 302 is fixedly connected to one side of the support plate 301. A sliding hole 303 is provided on the upper surface of the support plate 301. A slider 305 is slidably connected to the inner wall of the sliding hole 303. A screw rod 304 is threaded through the side of the slider 305. The output end of the servo motor 302 is fixedly connected to one end of the screw rod 304. A connecting plate 306 is fixedly connected to the upper surface of the slider 305. The servo motor 302 is started to drive the screw rod 304 to rotate. The screw rod 304 drives the slider 305 to move along the inner wall of the sliding hole 303. The slider 305 drives the connecting plate 306. The connecting plate 306 drives the automatic liquid transfer gun 314 above it, so that the automatic liquid transfer gun 314 can automatically move horizontally.

[0033] Both sides of the connecting plate 306 are fixedly connected with a fixing seat 307, and the inner wall of the fixing seat 307 is rotatably connected with a transmission wheel 308, one side of one of the fixing seats 307 is fixedly connected with a motor seat 309, and the inner wall of the motor seat 309 is fixedly connected with a driving motor 310, and the output end of the driving motor 310 is fixedly connected with one end of one of the transmission wheels 308, and the arc surface of the two transmission wheels 308 is transmission-connected with a conveyor belt 311. The upper surface of the detection dish 320 fits with the conveyor belt 311 to avoid liquid splashing during the dripping process. The surface of the conveyor belt 311 is provided with a circular hole 312, and the inner wall of the circular hole 312 is fixedly connected with a cylinder 313, and an automatic liquid transfer gun 314 is located inside the cylinder 313. The driving motor 310 is started to drive the transmission wheel 308 to rotate, the transmission wheel 308 drives the conveyor belt 311 to operate, the conveyor belt 311 drives the cylinder 313, and the cylinder 313 drives the automatic liquid transfer gun 314 to move, so that the automatic liquid transfer gun 314 can automatically move longitudinally.

[0034] In summary, the longitudinal movement and the lateral movement of the automatic liquid transfer gun 314 cooperate with each other, so that the automatic liquid transfer gun 314 can be automatically adjusted to drip the liquid to be tested into each detection cavity 321 on the detection dish 320 .

[0035] The outer wall of the cylinder 313 is fixedly connected with a positioning plate 315, and a positioning rod 316 is slidably penetrated on the side of the positioning plate 315. Both ends of the positioning rod 316 are fixedly connected with a fixing plate 317, and the two fixing plates 317 are respectively fixedly connected to the sides of the two fixing seats 307 that are away from each other. When the conveyor belt 311 drives the cylinder 313 and the automatic liquid transfer gun 314 to move, the positioning plate 315 fixed on the cylinder 313 will move along the arc surface of the positioning rod 316, thereby improving the stability of the cylinder 313 driving the automatic liquid transfer gun 314 to move, improving the stability of the dripping process, and preventing the automatic liquid transfer gun 314 and the cylinder 313 from shaking due to the excessive center of gravity.

[0036] The arc surface of the automatic liquid transfer gun 314 is fixedly connected with a plurality of rectangular blocks 319, and the upper end of the cylinder 313 is provided with a plurality of rectangular holes 318, and the rectangular blocks 319 fit the inner walls of the rectangular holes 318. Before the dripping operation, the rectangular blocks 319 fixed on the automatic liquid transfer gun 314 can be placed into the rectangular holes 318, so that the automatic liquid transfer gun 314 can be quickly supported and positioned.

[0037] A guide block 322 is fixedly connected to one side of the detection dish 320 away from the driving plate 6, and the guide block 322 is set to a wedge-shaped structure. By setting the guide block 322 with a wedge-shaped structure on one side of the detection dish 320, the process of moving the detection dish 320 to the inside of the conveyor belt 311 can be smoother, and the transmission belt can be prevented from jamming the detection dish 320 and making it unable to move.

[0038] The effect achieved by the entire automatic dripping mechanism 3 is that two effects are achieved by setting up the automatic pipetting mechanism: first, the pipette gun can be automatically controlled to drip the liquid to be tested into the multiple detection chambers 321 of the detection dish 320. The automatic dripping process makes the experimental process more coherent and systematic, reduces the operation steps and time waste, improves the detection efficiency, and reduces the errors caused by human operation, thereby improving the reliability of the experimental results; second, during the dripping process, the conveyor belt 311 can seal and shield the detection chamber 321, thereby avoiding the splashing of the detection liquid during the dripping process, thereby facilitating the loss of samples and avoiding pollution of the experimental environment.

[0039] like Figures 7 to 9 As shown, the automatic conveying mechanism 4 includes a welding plate 401 and a carrying plate 411, one side of the welding plate 401 is fixedly connected to the machine body 1, and the side of the welding plate 401 away from the machine body 1 is rotatably connected with a driving reel 404 and a driven reel 405, the side of the welding plate 401 away from the machine body 1 is fixedly connected with a limiting plate 402, one side of the limiting plate 402 is fixedly connected with a motor 403, the output end of the motor 403 is fixedly connected with the driving reel 404, and the arc surface of the driving reel 404 and the driven reel 405 is transmission-connected with a belt 406, a threaded rod 407 is rotatably penetrated on the side of the welding plate 401, one end of the threaded rod 407 is fixedly connected with the driven reel 405, the arc surface of the threaded rod 407 is threadedly connected with an ear plate 408, the upper surface of the ear plate 408 is fixedly connected with a conveying frame 409, and the cross-section of the conveying frame 409 is "C" shaped. The motor 403 is started to drive the active reel 404 to rotate. The active reel 404 drives the driven reel 405 to rotate with the help of the belt 406. The driven reel 405 drives the threaded rod 407 to rotate. The threaded rod 407 drives the conveyor frame 409 to move, so that the conveyor frame 409 is used to automatically push the test dish 320.

[0040] One side of the carrier plate 411 is in contact with the driving plate 6, the upper surface of the carrier plate 411 and the upper surface of the driving plate 6 are located in the same plane, the lower surface of the carrier plate 411 is fixedly connected with an auxiliary plate 412, and one side of the auxiliary plate 412 is fixedly connected with a side of the support plate 301 close to the driving plate 6. During the pushing process of the detection dish 320, the carrier plate 411 can always support the detection dish 320.

[0041] In summary, when the detection dish 320 needs to be automatically pushed from the driving plate 6 to the position to be dripped, the motor 403 is first started to drive the active reel 404 to rotate, the active reel 404 drives the driven reel 405 to rotate with the help of the belt 406, the driven reel 405 drives the threaded rod 407 to rotate, the threaded rod 407 drives the conveying frame 409 to move, and the conveying frame 409 pushes the detection dish 320 on the driving plate 6, so that the detection dish 320 moves to the surface of the driving plate 6 and the carrying plate 411 in sequence. The inside of the conveyor belt 311 can then perform the dripping operation. When the dripping operation is completed, the motor 403 is started to drive the active reel 404 to rotate in the opposite direction. The active reel 404 drives the driven reel 405 to rotate with the help of the belt 406. The driven reel 405 drives the threaded rod 407 to rotate. The threaded rod 407 drives the conveyor frame 409 to move. The conveyor frame 409 will push the detection dish 320 on the carrier plate 411, so that the detection dish 320 moves from the surface of the carrier plate 411 to the driving plate 6.

[0042] A guide rod 410 is slidably provided on one side of the conveying rack 409, and one end of the short arm end of the guide rod 410 is fixedly connected to the welding plate 401. When the threaded rod 407 drives the conveying rack 409 to move, the guide rod 410 can guide and limit the conveying rack 409, thereby avoiding deviation during the movement of the conveying rack 409.

[0043] A first limiting hole 413 is provided on the upper surface of the carrying plate 411, and a second limiting hole 414 is provided on the upper surface of the driving plate 6. The first limiting hole 413 is communicated with the second limiting hole 414. The inner wall of the first limiting hole 413 is slidably connected to a limiting block 415. The limiting block 415 is fixedly connected to the lower surface of the detection dish 320. When the conveying frame 409 pushes the detection dish 320 to move along the upper surface of the carrying plate 411 or the driving plate 6, the limiting block 415 fixed on the detection dish 320 will move along the inner walls of the first limiting hole 413 and the second limiting hole 414, thereby avoiding deviation of the detection dish 320 during the movement process.

[0044] The effect achieved by the entire automatic conveying mechanism 4 is that, by setting up the automatic conveying mechanism 4, the detection dish 320 can be automatically conveyed from the driving plate 6 to the automatic dripping mechanism 3, and after the dripping operation is completed, the detection dish 320 can be automatically conveyed from the automatic dripping mechanism 3 to the driving plate 6, thereby further improving the degree of automation of the microbial fluorescence detection system and further improving the detection efficiency.

[0045] like Fig.10 and Fig.11As shown, the sealing mechanism 5 includes a rotating seat 501, one side of the rotating seat 501 is fixedly connected to the body 1, a rotating shaft 502 is rotatably penetrated through the inner wall of the rotating seat 501, a connecting plate 503 is fixedly connected to the circular arc surface of the rotating shaft 502, a sealing plate 504 is fixedly connected to one side of the connecting plate 503, an auxiliary hole 505 is provided on the side of the sealing plate 504, an arc hole 507 is provided on one side of the rotating seat 501, the center of the arc hole 507 is coaxial with the axis of rotation of the rotating shaft 502, a fixing rod 508 is slidably connected to the inner wall of the arc hole 507, one end of the fixing rod 508 is fixedly connected to one side of the connecting plate 503, and an adjusting ring 509 is threadedly connected to the circular arc surface of the fixing rod 508. When the sealing plate 504 is needed to block the detection hole 2 during the detection process, first pull the auxiliary hole 505 to drive the sealing plate 504, and the sealing plate 504 will drive the connecting plate 503 at this time, so that the connecting plate 503 drives the rotating shaft 502 to rotate along the inner wall of the rotating seat 501, and at the same time, the fixing rod 508 on the connecting plate 503 will slide along the inner wall of the arc hole 507. When the sealing plate 504 blocks the detection hole 2, rotate the adjusting ring 509 to abut against the rotating seat 501 to complete the sealing operation of the detection hole 2.

[0046] A rubber pad 510 is fixedly connected to one side of the adjusting ring 509 close to the rotating seat 501. The diameter of the rubber pad 510 is larger than the diameter of the adjusting ring 509. The rubber pad 510 can increase the friction force of the adjusting ring 509 close to the rotating seat 501, thereby achieving the effect of enabling the adjusting ring 509 to be firmly in contact with the rotating seat 501 with the help of the rubber pad 510.

[0047] The arc surface of the rotating shaft 502 is covered with a coil spring 506, and the two ends of the coil spring 506 are fixedly connected to the connecting plate 503 and the rotating seat 501 respectively. After the rotating adjustment ring 509 is separated from the rotating seat 501, the coil spring 506 can automatically drive the sealing plate 504 to rotate in the direction away from the detection hole 2, thereby achieving the effect of quickly opening the sealing plate 504.

[0048] The effect achieved by the entire sealing mechanism 5 is that, by setting the sealing mechanism 5, after the driving plate 6 drives the detection dish 320 to move into the body 1, personnel can conveniently adjust the sealing plate 504 to block the detection hole 2, thereby sealing the detection hole 2, thereby further improving the practicality of the microbial fluorescence detection system.

[0049] The overall working principle is that when the detection system needs to be used to detect or quantify microorganisms, the following steps need to be performed: S1, first drive the plate 6 to move from the inside of the detection hole 2, and when the second limiting hole 414 on the drive plate 6 is aligned with the first limiting hole 413 on the carrier plate 411, stop the movement of the drive plate 6, and then start the motor 403 to drive the active reel 404 to rotate, the active reel 404 drives the driven reel 405 to rotate with the help of the belt 406, the driven reel 405 drives the threaded rod 407 to rotate, the threaded rod 407 drives the conveyor frame 409 to move, the conveyor frame 409 will push the detection dish 320 on the drive plate 6, so that the detection dish 320 moves along the surface of the drive plate 6 and the carrier plate 411 to the inside of the conveyor belt 311 in turn, so that the detection dish 320 can be automatically pushed from the drive plate 6 to the position to be dripped; S2. After the detection dish 320 is automatically pushed from the driving plate 6 to the position to be dripped, the servo motor 302 is started to drive the screw rod 304 to rotate, the screw rod 304 drives the slider 305 to move along the inner wall of the sliding hole 303, the slider 305 drives the connecting plate 306, the connecting plate 306 drives the automatic liquid transfer gun 314 above it, so that the automatic liquid transfer gun 314 can automatically move laterally, the driving motor 310 is started to drive the transmission wheel 308 to rotate, the transmission wheel 308 drives the conveyor belt 311 to operate, the conveyor belt 311 drives the cylinder 313, the cylinder 313 drives the automatic liquid transfer gun 314 to move, so that the automatic liquid transfer gun 314 can automatically move longitudinally, the longitudinal movement and the lateral movement of the automatic liquid transfer gun 314 cooperate with each other, and the automatic liquid transfer gun 314 can be automatically adjusted to drip 50μl of the liquid to be detected into each detection cavity 321 on the detection dish 320.

[0050] S3. When the dripping operation is completed, the motor 403 is started to drive the active reel 404 to rotate in the opposite direction. The active reel 404 drives the driven reel 405 to rotate with the help of the belt 406. The driven reel 405 drives the threaded rod 407 to rotate. The threaded rod 407 drives the conveying frame 409 to move. The conveying frame 409 pushes the detection dish 320 on the carrying plate 411, so that the detection dish 320 moves from the surface of the carrying plate 411 to the driving plate 6.

[0051] S4. When the detection dish 320 moves from the surface of the carrier plate 411 to the driving plate 6, the driving plate 6 is controlled to drive the detection dish 320 into the body 1, and the delivery pump inside the body 1 is started to introduce the fluorescent dye into the detection, so that the detection operation can be carried out and the detection can be completed within 27 minutes.

[0052] The above are only preferred embodiments of the present invention and are not intended to limit the present invention in other forms. Any technician familiar with the profession may use the technical contents disclosed above to change or modify them into equivalent embodiments with equivalent changes and apply them to other fields. However, any simple modification, equivalent change and modification made to the above embodiments based on the technical essence of the present invention without departing from the technical solution of the present invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. A microbial fluorescence detection system, comprising a body (1), a detection hole (2), a drive plate (6), a mounting plate (7), a containing bottle (8), a conduit (9) and an automatic dripping mechanism (3), characterized in that: The detection hole (2) is arranged on one side of the machine body (1); the drive plate (6) is installed inside the detection hole (2); the mounting plate (7) is installed on the machine body (1); a plurality of containing bottles (8) are installed on one side of the mounting plate (7); one end of the guide tube (9) is connected to the containing bottles (8); the automatic dripping mechanism (3) is located on one side of the machine body (1); the automatic dripping mechanism (3) comprises a support plate (301), a detection dish (320) and an automatic liquid transfer gun (314); the upper surface of the detection dish (320) A plurality of detection cavities (321) are provided on the surface of the detection dish (320), the detection cavities (321) are evenly distributed on the upper surface of the detection dish (320), the lower surface of the detection dish (320) and the upper surface of the driving plate (6) are located in the same plane, one side of the support plate (301) is fixedly connected to the body (1), one side of the support plate (301) is fixedly connected to a servo motor (302), the upper surface of the support plate (301) is provided with a sliding hole (303), the inner wall of the sliding hole (303) is slidably connected to a slider (305), and the A screw rod (304) is threadedly penetrated on the side surface of the slider (305); the output end of the servo motor (302) is fixedly connected to one end of the screw rod (304); a connecting plate (306) is fixedly connected to the upper surface of the slider (305); both sides of the connecting plate (306) are fixedly connected to fixing seats (307); the inner wall of the fixing seat (307) is rotatably connected to a transmission wheel (308); one side of the fixing seat (307) is fixedly connected to a motor seat (309); the inner wall of the motor seat (309) is fixedly connected to the inner wall of the fixing seat (307); A driving motor (310) is fixedly connected, the output end of the driving motor (310) is fixedly connected to one end of one of the transmission wheels (308), the arc surfaces of the two transmission wheels (308) are transmission-connected to a conveyor belt (311), the upper surface of the detection dish (320) is in contact with the conveyor belt (311), a circular hole (312) is provided on the surface of the conveyor belt (311), a cylinder (313) is fixedly connected to the inner wall of the circular hole (312), and the automatic liquid transfer gun (314) is located inside the cylinder (313).

2. A microbial fluorescence detection system according to claim 1, characterized in that: A positioning plate (315) is fixedly connected to the outer wall of the cylinder (313), a positioning rod (316) is slidably penetrated through the side surface of the positioning plate (315), both ends of the positioning rod (316) are fixedly connected to fixing plates (317), and the two fixing plates (317) are respectively fixedly connected to the sides of the two fixing seats (307) that are away from each other.

3. A microbial fluorescence detection system according to claim 1, characterized in that: A plurality of rectangular blocks (319) are fixedly connected to the arc surface of the automatic liquid transfer gun (314); a plurality of rectangular holes (318) are opened at the upper end of the cylinder (313); and the rectangular blocks (319) fit in contact with the inner walls of the rectangular holes (318).

4. A microbial fluorescence detection system according to claim 1, characterized in that: A guide block (322) is fixedly connected to a side of the detection dish (320) away from the drive plate (6), and the guide block (322) is configured as a wedge-shaped structure.

5. A microbial fluorescence detection system according to claim 1, characterized in that: An automatic conveying mechanism (4) is provided on a side of the machine body (1) away from the support plate (301), the automatic conveying mechanism (4) comprising a welding plate (401) and a bearing plate (411), one side of the welding plate (401) being fixedly connected to the machine body (1), the side of the welding plate (401) away from the machine body (1) being rotatably connected to a driving reel (404) and a driven reel (405), the side of the welding plate (401) away from the machine body (1) being fixedly connected to a limiting plate (402), one side of the limiting plate (402) being fixedly connected to a motor (403), the output end of the motor (403) being fixedly connected to the driving reel (404), the arc surface of the driving reel (404) and the driven reel (405) being transmission-connected by a belt ( 406), a threaded rod (407) is rotatably penetrated on the side of the welding plate (401), one end of the threaded rod (407) is fixedly connected to the driven reel (405), the arc surface of the threaded rod (407) is threadedly connected to an ear plate (408), the upper surface of the ear plate (408) is fixedly connected to a conveying frame (409), the cross-section of the conveying frame (409) is "C" shaped, one side of the bearing plate (411) is in contact with the driving plate (6), the upper surface of the bearing plate (411) and the upper surface of the driving plate (6) are located in the same plane, the lower surface of the bearing plate (411) is fixedly connected to an auxiliary plate (412), and one side of the auxiliary plate (412) is fixedly connected to a side of the support plate (301) close to the driving plate (6).

6. A microbial fluorescence detection system according to claim 5, characterized in that: A guide rod (410) is slidably provided on one side of the conveying frame (409), and one end of the short arm end of the guide rod (410) is fixedly connected to the welding plate (401).

7. A microbial fluorescence detection system according to claim 5, characterized in that: A first limiting hole (413) is formed on the upper surface of the bearing plate (411), a second limiting hole (414) is formed on the upper surface of the driving plate (6), the first limiting hole (413) is connected to the second limiting hole (414), an inner wall of the first limiting hole (413) is slidably connected to a limiting block (415), and the limiting block (415) is fixedly connected to the lower surface of the detection dish (320).

8. A microbial fluorescence detection system according to claim 1, characterized in that: A sealing mechanism (5) is provided on one side of the machine body (1) close to the detection hole (2), the sealing mechanism (5) comprising a rotating seat (501), one side of the rotating seat (501) being fixedly connected to the machine body (1), a rotating shaft (502) being rotatably penetrated through the inner wall of the rotating seat (501), a connecting plate (503) being fixedly connected to the circular arc surface of the rotating shaft (502), a sealing plate (504) being fixedly connected to one side of the connecting plate (503), and the sealing plate (504) An auxiliary hole (505) is provided on the side of the rotating seat (504), an arc hole (507) is provided on one side of the rotating seat (501), the center of the arc hole (507) is coaxial with the axis of rotation of the rotating shaft (502), a fixing rod (508) is slidably connected to the inner wall of the arc hole (507), one end of the fixing rod (508) is fixedly connected to one side of the connecting plate (503), and an adjusting ring (509) is threadedly connected to the arc surface of the fixing rod (508), A rubber pad (510) is fixedly connected to one side of the adjusting ring (509) close to the rotating seat (501); the diameter of the rubber pad (510) is greater than the diameter of the adjusting ring (509).

9. A microbial fluorescence detection system according to claim 8, characterized in that: The arc surface of the rotating shaft (502) is sleeved with a coil spring (506), and the two ends of the coil spring (506) are respectively fixedly connected to the connecting plate (503) and the rotating seat (501).

10. A method for detecting microorganism fluorescence according to any one of claims 1 to 8, characterized in that: Includes steps: 1) Use the automatic pipette to transfer 50 μL of the pre-test sample into each test cavity, and then place the test dish into the microbial fluorescence test; 2) Select the test conditions and obtain the test results in real time. The test results can be obtained within 27 minutes.

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