A detection device and method for detecting tauroursodeoxycholic acid in freeze-dried bear bile

Abstract

The present invention relates to the technical field of tauroursodeoxycholic acid detection, specifically, to a tauroursodeoxycholic acid detection device in bear bile freeze-dried product and a detection method thereof, comprising a high-efficiency chromatograph for detecting tauroursodeoxycholic acid, a mobile phase solution bottle fixed on the top surface of the high-efficiency chromatograph for containing a mobile phase, an external pipe connected to the right side of the mobile phase solution bottle, a sample bottle assembly is provided on the right side of the high-efficiency chromatograph, a clamping assembly for clamping the sample bottle assembly is provided on the middle of the right side wall of the high-efficiency chromatograph, and a bottom support assembly for supporting the sample bottle assembly is provided on the right side wall of the high-efficiency chromatograph below the clamping assembly. The device can clamp and fix the bottle body during multiple measurements to prevent the bottle body from being knocked over during use by setting the clamping assembly; by setting the sample bottle assembly and the bottom support assembly, the sample solution can be added again during multiple measurements by simply turning a knob, which is simple to use and easy to operate.

Description

Technical Field

[0001] The invention belongs to the technical field of tauroursodeoxycholic acid detection, and specifically relates to a device for detecting tauroursodeoxycholic acid in freeze-dried bear bile and a detection method thereof. Background Art

[0002] Tauroursodeoxycholic acid is an important drug ingredient that plays an important role in reducing the saturation of cholesterol in bile, promoting the secretion of bile acid, and increasing the solubility of cholesterol in bile. Tauroursodeoxycholic acid is generally extracted from freeze-dried bear bile. Before extraction, the content of tauroursodeoxycholic acid in freeze-dried bear bile needs to be determined by high performance liquid chromatography.

[0003] For example, the invention with authorization announcement number CN118549572A discloses a high performance liquid chromatograph that can avoid sample residue in the technical field related to chromatographs, including: a chromatograph body, a detection carrying cavity is provided in the chromatograph body, a detection host part is provided on the side of the detection carrying cavity, a host control cavity connected to the detection carrying cavity is provided in the detection host part, a detection system is embedded and fixedly installed in the host control cavity, a control host is fixedly installed on the top of the detection system, and a solvent delivery system, a sample injection mechanism, and a liquid storage function mechanism are fixedly installed in the detection carrying cavity from bottom to top.

[0004] Combining the above cases and actual conditions, we found the following problems: When measuring the content of tauroursodeoxycholic acid in freeze-dried bear bile, multiple measurements are generally required to ensure the accuracy of the data. During the measurement operation, placing the sample bottle too close can easily knock over the sample bottle, and placing the sample bottle too far can affect the measurement efficiency and make sampling inconvenient. At the same time, during the process of tipping the sample bottle for sampling, it is easy to spill, making the operation troublesome. Summary of the invention

[0005] The object of the present invention is to provide a device and method for detecting tauroursodeoxycholic acid in freeze-dried bear bile, so as to solve the above problems in the prior art.

[0006] To achieve the above-mentioned purpose, the present invention provides a device for detecting tauroursodeoxycholic acid in freeze-dried bear bile, comprising a high-efficiency chromatograph for detecting tauroursodeoxycholic acid, a mobile phase solution bottle fixed on the top surface of the high-efficiency chromatograph for containing a mobile phase, the right side of the mobile phase solution bottle is connected with an external pipe, a sample bottle assembly is arranged on the right side of the high-efficiency chromatograph, a clamping assembly for clamping the sample bottle assembly is arranged on the middle of the right side wall of the high-efficiency chromatograph, and a bottom support assembly for holding the sample bottle assembly is arranged on the right side wall of the high-efficiency chromatograph below the clamping assembly;

[0007] The clamping assembly comprises a fixed block fixed to the right side wall of the high-efficiency chromatograph, a movable structure arranged on the right side of the fixed block, four groups of inclined rods symmetrically arranged on the front and rear sides of the movable structure in pairs, and two clamping claws arranged at the right ends of the corresponding inclined rods. The movable structure comprises a movable block and two connecting plates symmetrically arranged on the front and rear sides of the movable block, the two groups of inclined rods on the same side are distributed parallel up and down, and the inclined rods in the same group are arranged parallel front and back, one end of the connecting plate is hinged to the movable block, and the other end extends between the two groups of inclined rods on the corresponding same side and is hinged to the two groups of inclined rods on the same side. The left side wall of the movable block is symmetrically provided with movable cylinders front and back, the right side wall of the fixed block is provided with a sliding cavity corresponding to the movable cylinder, a plug rod is fixed to the left inner wall of the sliding cavity, a spring is provided on the outer sleeve of the plug rod, and the plug rod is inserted into the corresponding movable cylinder;

[0008] A spring plate is fixed to the middle part of the left side wall of the movable block, a clamping rod is fixed to the front end of the bottom surface of the spring plate, a spring plate groove corresponding to the position and size of the spring plate is provided in the middle part of the fixed block, a straight groove is provided on the right side of the bottom surface of the spring plate groove, a first curved groove is provided on the rear side of the left end of the straight groove, and a second curved groove is provided on the front side, the first curved groove and the second curved groove are arranged in a diamond shape, and an arc-shaped clamping socket is provided on the left end of the protruding part between the first curved groove and the second curved groove.

[0009] In the technical solution of the present invention, the two oblique rods in the same group are arranged in parallel, and the inner oblique rod is positioned to the left relative to the outer oblique rod, the left end of the oblique rod is hinged to the fixed block, and the right end is fixedly connected to the left end of the clamp, and the clamp is arranged tilted inward.

[0010] In the technical solution of the present invention, the bottom surface of the first curved groove gradually increases in height from the right end to the left end, and the bottom surface of the second curved groove gradually decreases in height from the right end to the left end. The bottom surface of the first curved groove smoothly transitions with the straight groove, and a pad is fixed in the middle of the left side wall where the left ends of the first curved groove and the second curved groove meet.

[0011] In the technical solution of the present invention, the sample bottle assembly includes a bottle body and a connecting tube fixed in the middle of the bottle body, the top of the connecting tube is connected to the external tube by a hose, a control valve is provided in the connecting tube near the top, a first piston is provided in the bottle body, the connecting tube passes through the first piston and the top surface of the bottle body from bottom to top, the first piston is slidably connected to the connecting tube, a plurality of openings are provided at the bottom end of the connecting tube, and a liquid inlet is provided near the bottom of the side wall of the bottle body.

[0012] In the technical solution of the present invention, an air cavity is provided in the middle of the bottom surface of the bottle body, a second piston with matching size is provided in the air cavity, limiting rods are symmetrically provided in the air cavity, the limiting rods pass through the second piston and the two are slidably connected, and air flow channels are symmetrically provided in the side wall of the bottle body.

[0013] In the technical solution of the present invention, the top of the airflow channel is connected to the top of the bottle body, and the bottom end is connected to the top of the air cavity. A ring plate is provided on the inner wall of the bottle body below the top of the airflow channel. The lowest height of the first piston is higher than the opening and the highest height is lower than the ring plate. The highest height of the second piston is less than the height of the bottom end of the airflow channel.

[0014] In the technical solution of the present invention, the base support assembly includes a base and an extrusion structure arranged in the base for squeezing the sample solution into the high-efficiency chromatograph, the middle of the top surface of the base is provided with left and right horizontal bottle grooves, the bottom of the bottle body is adapted to the bottle grooves, and an active cavity is provided in the base below the bottle groove, and the extrusion structure is arranged in the active cavity.

[0015] In the technical solution of the present invention, the extrusion structure includes a mounting seat, a worm gear arranged above the mounting seat, and a threaded rod coaxially connected to the top of the worm gear. Left and right horizontal sliding rods are provided near the bottom of the movable cavity. The sliding rods pass through the mounting seat and the two are slidably connected. The middle part of the bottom surface of the worm gear is embedded in the top surface of the mounting seat and the worm gear can rotate freely. A meshing worm is provided on the left side of the worm gear. A knob is coaxially connected to the front end of the worm shaft. Support rods are symmetrically fixed to the side walls of the mounting seat front and back. The worm shaft passes through the support rods and the worm shaft can rotate.

[0016] In the technical solution of the present invention, a push rod is provided on the outer sleeve of the threaded rod, the threaded rod is threadedly connected to the middle part of the push rod, the side wall of the push rod is provided with a flange, the bottom surface of the air cavity is provided with a notch matched with the position and size of the flange, a push rod groove matched with the push rod is provided between the bottle groove and the active cavity, and the front side wall of the active cavity is provided with a shaft rod groove matched with the worm shaft.

[0017] On the other hand, the present invention also provides a method for detecting tauroursodeoxycholic acid in freeze-dried bear bile, using the above-mentioned tauroursodeoxycholic acid detection device in freeze-dried bear bile, comprising the following steps:

[0018] S1. Dissolve the bear bile freeze-dried product in the ammonium chloride aqueous solution in proportion, inject it into the bottle from the liquid inlet after it is completely dissolved, and connect the connecting pipe and the external pipe through a hose;

[0019] S2, align the notch at the bottom of the bottle body with the flange of the push rod and put it into the bottle groove, then push the bottle body to the left to squeeze the movable block, and the movable block moving to the left pulls the oblique rods on both sides to rotate inward through the connecting plate, thereby driving the clamping claws to clamp the bottle body;

[0020] S3, the movable block moving to the left drives the clamping rod along the straight groove into the first curved groove, and enters the clamping mouth under the guidance of the pad block, and then you can let go;

[0021] S4. During the test, the tester turns the knob, and the threaded rod is driven to rotate by the worm and worm gear transmission, so that the push rod pushes the second piston upward, and the gas in the air flow channel pushes the first piston downward, so that the sample solution is pressed from the opening into the connecting pipe and finally into the external pipe from the hose. At the same time, the pump of the mobile phase solution bottle is started, and the mobile phase and the sample solution enter the high-performance chromatograph for testing;

[0022] S5. After one determination, the mobile phase solution is reintroduced to clean the chromatographic column in the high performance chromatograph, and then the knob is rotated again to perform a second determination of tauroursodeoxycholic acid. Repeating the above S4 step can perform multiple determinations of the sample.

[0023] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0024] 1. In the present invention, by setting a clamping assembly, when in use, the notch at the bottom of the bottle body is aligned with the flange of the push rod and placed in the bottle groove, and then the bottle body is pushed to the left to squeeze the movable block, and the movable block moving to the left pulls the inclined rods on both sides to rotate inward through the connecting plate, thereby driving the clamping claws to clamp the bottle body, and the movable block moving to the left drives the clamping rod to enter the first curved groove along the straight groove, and enters the clamping mouth under the guidance of the cushion block, and you can let go at this time. After the measurement is completed, push the bottle body to the left again, so that the clamping rod enters the second curved groove under the action of the front half of the cushion block, and returns to the straight groove under the action of the spring to release the clamping, and the operation is simple and convenient.

[0025] 2. In the present invention, during detection, the detection personnel rotate the knob, and the threaded rod is driven to rotate by the worm and the worm gear transmission, so that the push rod pushes the second piston upward, and the gas in the air flow channel pushes the first piston downward, so that the sample solution is pressed into the connecting pipe from the opening and finally enters the external pipe from the hose. At the same time, the pump of the mobile phase solution bottle is started, and the mobile phase carries the sample solution into the high-performance chromatograph for detection. After one measurement, the knob is rotated again to perform a secondary measurement of tauroursodeoxycholic acid. The above steps can be repeated to perform multiple measurements of the sample. The operation is simple and convenient, and there will be no spillage. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0027] Figure 2 is a schematic diagram of a clamping assembly of the present invention;

[0028] Figure 3 It is a cross-sectional view of the clamping assembly in the present invention;

[0029] Figure 4 It is a cross-sectional view of a fixing block in the present invention;

[0030] Figure 5 For the present invention Figure 4 Enlarged view of point A in the middle;

[0031] Figure 6 For the present invention Figure 4 Enlarged view of point B in the middle;

[0032] Figure 7 It is an exploded view of the active structure in the present invention;

[0033] Figure 8 It is a schematic diagram of the sample bottle assembly and the base assembly in the present invention;

[0034] Fig. 9 It is a cross-sectional view of a sample bottle assembly in the present invention;

[0035] Fig.10 For the present invention Fig. 9 Enlarged view of point C in the middle;

[0036] Fig.11 It is a cross-sectional view of the base in the present invention;

[0037] Fig.12 It is an exploded diagram of the extrusion structure in the present invention;

[0038] Description of reference numerals:

[0039] 1. High efficiency chromatograph;

[0040] 2. Mobile phase solution bottle;

[0041] 3. External pipe;

[0042] 4. Clamping assembly; 41. Fixed block; 411. Straight groove; 412. First curved groove; 413. Pad; 414. Second curved groove; 415. Insert rod; 416. Spring; 417. Bayonet; 42. Movable structure; 421. Movable block; 422. Connecting plate; 423. Movable cylinder; 424. Spring plate; 425. Clamping rod; 43. Oblique rod; 44. Clamping claw;

[0043] 5. Sample bottle assembly; 51. Bottle body; 511. Air cavity; 512. Ring plate; 513. Notch; 514. Limit rod; 52. Connecting pipe; 521. Opening; 53. First piston; 54. Air flow channel; 55. Second piston;

[0044] 6. Bottom bracket assembly; 61. Base; 611. Bottle slot; 612. Axle slot; 613. Push rod slot; 614. Movable cavity; 615. Slide rod; 62. Extrusion structure; 621. Mounting seat; 622. Support rod; 623. Worm gear; 624. Threaded rod; 625. Push rod; 626. Worm; 627. Knob. DETAILED DESCRIPTION

[0045] The specific implementation modes of the present invention are described in detail below in conjunction with the accompanying drawings, but it should be understood that the protection scope of the present invention is not limited by the specific implementation modes.

[0046] Unless explicitly stated otherwise, throughout the specification, the term “comprise” or variations thereof such as “include” or “comprising” will be understood as including the stated elements or components but not excluding other elements or components.

[0047] Reference Figure 1-Figure 9 As shown, this embodiment provides a technical solution:

[0048] A device for detecting tauroursodeoxycholic acid in freeze-dried bear bile comprises a high-efficiency chromatograph 1 for detecting tauroursodeoxycholic acid, a mobile phase solution bottle 2 fixed on the top surface of the high-efficiency chromatograph 1 for containing a mobile phase, an external pipe 3 is connected to the right side of the mobile phase solution bottle 2, a sample solution is driven into the high-efficiency chromatograph 1 for analysis by the mobile phase solution, a chromatogram of the high-efficiency chromatograph 1 is integrated by a chromatographic software workstation, parameters such as peak area or peak height ratio are calculated, and the content of tauroursodeoxycholic acid in the sample is calculated according to an external standard method or an internal standard method;

[0049] A sample bottle assembly 5 is provided on the right side of the high-efficiency chromatograph 1. A clamping assembly 4 for clamping the sample bottle assembly 5 is provided near the middle of the right side wall of the high-efficiency chromatograph 1. The sample bottle assembly 5 is fixed by providing the clamping assembly 4 to prevent the sample bottle assembly 5 from being knocked over during operation. A bottom support assembly 6 for supporting the sample bottle assembly 5 is provided below the clamping assembly 4 on the right side wall of the high-efficiency chromatograph 1. The bottom support assembly 6 can support the sample bottle assembly 5 and is also convenient for multiple measurements during use. The sample solution can be added again by simply turning the knob 627.

[0050] The clamping assembly 4 includes a fixed block 41 fixed to the right side wall of the high-efficiency chromatograph 1, a movable structure 42 arranged on the right side of the fixed block 41, four groups of inclined rods 43 symmetrically arranged on the front and rear sides of the movable structure 42, and two clamps 44 arranged on the right ends of the corresponding inclined rods 43. The movable structure 42 includes a movable block 421 and two connecting plates 422 symmetrically arranged on the front and rear sides of the movable block 421. The two groups of inclined rods 43 on the same side are distributed in parallel up and down, and the inclined rods 43 on the same group are arranged in parallel front and back. One end of the connecting plate 422 is hinged to the movable block 421, and the other end extends between the two groups of inclined rods 43 on the same side and is hinged to the two groups of inner inclined rods 43 on the same side. The left end of the movable block 421 The side wall is symmetrically provided with movable cylinders 423 at the front and rear sides, and the right side wall of the fixed block 41 is provided with a sliding cavity corresponding to the movable cylinder 423. A plug rod 415 is fixed to the inner wall of the left side of the sliding cavity. A spring 416 is provided on the outer sleeve of the plug rod 415. The plug rod 415 is inserted into the corresponding movable cylinder 423. When in use, the inspector pushes the bottle body 51 to the left to squeeze the movable block 421. The movable block 421 moving to the left pulls the inclined rods 43 on both sides to rotate inward through the connecting plate 422, thereby driving the clamping claw 44 to clamp the bottle body 51. The spring 416 can automatically pop out the movable block 421 after the clamping rod 425 is separated from the clamping port 417, thereby releasing the fixation of the bottle body 51, which is convenient for the operator to use;

[0051] A spring plate 424 is fixed to the middle of the left side wall of the movable block 421, and a clamping rod 425 is fixed to the bottom surface of the spring plate 424 near the front end. A spring plate groove corresponding to the position and size of the spring plate 424 is provided in the middle of the fixed block 41, and a straight groove 411 is provided on the bottom surface of the spring plate groove near the right side. A first curved groove 412 is provided on the rear side of the left end of the straight groove 411, and a second curved groove 414 is provided on the front side. The first curved groove 412 and the second curved groove 414 are arranged in a diamond shape, and an arc-shaped clamping hole 417 is provided at the left end of the protruding part between the first curved groove 412 and the second curved groove 414. When clamping, the movable block 421 moving to the left drives the clamping rod 425 to enter the first curved groove 412 along the straight groove 411, and enters the clamping hole 417 under the guidance of the cushion block 413 and the restoring force of the spring 416. At this time, the positions of the spring plate 424 and the movable block 421 are fixed, so that the clamping claw 44 can stably clamp the bottle body 51.

[0052] Among them, Figure 1-Figure 3 as well as Figure 7 As shown, two inclined rods 43 of the same group are arranged in parallel, and the inner inclined rod 43 is positioned to the left relative to the outer inclined rod 43, so that when the inclined rod 43 rotates, it will drive the clamping claw 44 to rotate inward, avoiding the sample bottle assembly 5 from being squeezed out of the clamping range of the clamping claw 44. The left end of the inclined rod 43 is hinged to the fixed block 41, and the right end is fixedly connected to the left end of the clamping claw 44. The clamping claw 44 is arranged to be inclined inward, ensuring that the clamping force of the clamping claw 44 on the sample bottle assembly 5 is to the left, avoiding the sample bottle assembly 5 from being pushed out and affecting the clamping effect.

[0053] In addition, if Figure 4-Figure 6As shown, the height of the bottom surface of the first curved groove 412 gradually increases from the right end to the left end, and the height of the bottom surface of the second curved groove 414 gradually decreases from the right end to the left end, ensuring that the clamping rod 425 first enters the first curved groove 412 and then returns to the straight groove 411 from the second curved groove 414, and the bottom surface of the first curved groove 412 and the straight groove 411 have a smooth transition, and a cushion block 413 is fixed to the middle of the left side wall where the left ends of the first curved groove 412 and the second curved groove 414 meet, and the cushion block 413 plays a guiding role. When the clamping rod 425 reaches the left end of the first curved groove 412, the clamping rod 425 will enter the bayonet 417 and get stuck under the rightward restoring force of the spring 416, and then push the bottle body 51 to the left, so that the clamping rod 425 enters the second curved groove 414 under the action of the front half of the cushion block 413, and then returns to the straight groove 411 under the action of the spring 416 to release the clamping;

[0054] In addition, if Figure 8-Figure 10 As shown, the sample bottle assembly 5 includes a bottle body 51 and a connecting pipe 52 fixed in the middle of the bottle body 51. The top of the connecting pipe 52 is connected to the external pipe 3 through a hose, which can be connected through a threaded joint. A control valve is provided near the top of the connecting pipe 52. A first piston 53 is provided in the bottle body 51. The connecting pipe 52 passes through the first piston 53 and the top surface of the bottle body 51 from bottom to top. The first piston 53 is slidably connected to the connecting pipe 52. A plurality of openings 521 are provided at the bottom of the connecting pipe 52. A liquid inlet is provided near the bottom of the side wall of the bottle body 51. Before the test, the control valve is closed. The tester injects the sample solution into the bottle body 51 through the liquid inlet. At this time, the internal air pressure will push the first piston 53 upward.

[0055] Furthermore, an air cavity 511 is provided in the middle of the bottom surface of the bottle body 51, and a second piston 55 with a matching size is provided in the air cavity 511. Limiting rods 514 are symmetrically provided in the air cavity 511, and the limiting rods 514 pass through the second piston 55 and the two are slidably connected. An air flow channel 54 is symmetrically provided in the side wall of the bottle body 51. When the first piston 53 is lifted up, the air above the first piston 53 will press down the second piston 55 along the air flow channel 54, so that the second piston 55 returns to the bottom of the air cavity 511.

[0056] In addition, the top of the airflow channel 54 is connected to the top of the bottle body 51, and the bottom is connected to the top of the air cavity 511. The inner wall of the bottle body 51 is provided with a ring plate 512 below the top of the airflow channel 54. The lowest height of the first piston 53 is higher than the opening 521, and the highest height is lower than the ring plate 512, so as to prevent the first piston 53 from moving too high or too low, causing the airflow channel 54 to be connected to the space below the first piston 53 or the inside of the connecting pipe 52 to be connected to the space above the first piston 53, resulting in chaotic internal air pressure and inability to use. The highest height of the second piston 55 is less than the height of the bottom end of the airflow channel 54, so as to prevent the space below the second piston 55 from being connected to the airflow channel 54.

[0057] In addition, if Figure 11-Figure 12 As shown, the base assembly 6 includes a base 61 and an extrusion structure 62 disposed in the base 61 for extruding the sample solution into the high-efficiency chromatograph 1. The middle of the top surface of the base 61 is provided with a bottle groove 611 in the left and right directions. The bottom of the bottle body 51 is adapted to the bottle groove 611. An active cavity 614 is disposed below the bottle groove 611 in the base 61. The extrusion structure 62 is disposed in the active cavity 614. The bottle body 51 is placed in the bottle groove 611 to improve stability when sliding left and right.

[0058] Specifically, the extrusion structure 62 includes a mounting seat 621, a worm gear 623 arranged above the mounting seat 621, and a threaded rod 624 coaxially connected to the top of the worm gear 623. Left and right horizontal sliding rods 615 are arranged near the bottom of the movable cavity 614. The sliding rods 615 pass through the mounting seat 621 and the two are slidably connected. The mounting seat 621 is restricted by setting the sliding rods 615 to prevent the mounting seat 621 and the worm gear 623 from rotating synchronously, and at the same time, the mounting seat 621 will not be blocked from sliding. The middle part of the bottom surface of the worm gear 623 is embedded in the top surface of the mounting seat 621 and the worm gear 623 can rotate freely. A meshing worm 626 is provided on the left side of 623, and a knob 627 is coaxially connected to the front end of the rotating shaft of the worm 626. By turning the knob 627, the threaded rod 624 can be driven to rotate through the transmission of the worm 626 and the worm wheel 623. Support rods 622 are symmetrically fixed to the side wall of the mounting seat 621. The rotating shaft of the worm 626 passes through the support rod 622 and the rotating shaft of the worm 626 can rotate. The support rod 622 can support the worm 626, so that when the extrusion structure 62 slides left and right, the entire extrusion structure 62 will slide together to prevent the worm 626 and the worm wheel 623 from separating.

[0059] Furthermore, a push rod 625 is disposed on the outer sleeve of the threaded rod 624, and the threaded rod 624 is threadedly connected to the middle of the push rod 625, and a flange is disposed on the side wall of the push rod 625, and a notch 513 matching the position and size of the flange is disposed on the bottom surface of the air cavity 511. When the threaded rod 624 rotates, the bottle body 51 is clamped and fixed, and the push rod 625 cannot rotate under the restriction of the notch 513, which will drive the push rod 625 to move upward and lift the second piston 55, so that the gas in the air flow channel 54 pushes the second piston 55 downward. A piston 53 is provided, so that the sample solution is pressed into the connecting tube 52 from the opening 521 and finally enters the external tube 3 from the hose. A push rod groove 613 adapted to the push rod 625 is provided between the bottle groove 611 and the movable cavity 614. A shaft rod groove 612 adapted to the rotating shaft of the worm 626 is provided on the front side wall of the movable cavity 614. By setting the push rod groove 613 and the shaft rod groove 612, it is avoided that the rotating shafts of the push rod 625 and the worm 626 are blocked when the extrusion structure 62 slides left and right with the bottle body 51.

[0060] The present invention also provides a method for detecting tauroursodeoxycholic acid in freeze-dried bear bile, using the above-mentioned device for detecting tauroursodeoxycholic acid in freeze-dried bear bile, comprising the following steps:

[0061] S1, dissolving the bear bile freeze-dried product in an ammonium chloride aqueous solution in proportion, injecting it into the bottle body 51 from the liquid inlet after it is completely dissolved, and connecting the connecting pipe 52 and the external pipe 3 through a hose;

[0062] S2, align the notch 513 at the bottom of the bottle body 51 with the flange of the push rod 625 and put it into the bottle groove 611, then push the bottle body 51 to the left to squeeze the movable block 421, and the movable block 421 moving to the left pulls the inclined rods 43 on both sides to rotate inward through the connecting plate 422, thereby driving the clamping claws 44 to clamp the bottle body 51;

[0063] S3, the movable block 421 moving to the left drives the clamping rod 425 to enter the first curved groove 412 along the straight groove 411, and enters the clamping hole 417 under the guidance of the cushion block 413, and the user can release the clamping rod 425 at this time;

[0064] S4. During the test, the tester first opens the control valve of the connecting pipe 52, and then turns the knob 627. The threaded rod 624 is driven to rotate by the worm 626 and the worm gear 623, so that the push rod 625 pushes the second piston 55 upward, and the gas in the gas flow channel 54 pushes the first piston 53 downward, so that the sample solution is pressed into the connecting pipe 52 from the opening 521 and finally enters the external pipe 3 from the hose. At the same time, the pump of the mobile phase solution bottle 2 is started to bring the mobile phase and the sample solution into the high-performance chromatograph 1 for testing;

[0065] S5. After one determination, the mobile phase solution is reintroduced to clean the chromatographic column in the high performance chromatograph 1, and then the knob 627 is rotated again to perform a second determination of tauroursodeoxycholic acid. Repeating the above step S4 can perform multiple determinations of the sample.

[0066] The foregoing description of specific exemplary embodiments of the present invention is for the purpose of illustration and demonstration. These descriptions are not intended to limit the present invention to the precise form disclosed, and it is clear that many changes and variations can be made based on the above teachings. The purpose of selecting and describing exemplary embodiments is to explain the specific principles of the present invention and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the present invention and various different selections and changes. The scope of the present invention is intended to be limited by the specification and its equivalents.

Claims

1. A device for detecting tauroursodeoxycholic acid in freeze-dried bear bile, comprising a high-performance chromatograph (1) for detecting tauroursodeoxycholic acid, and a mobile phase solution bottle (2) fixed to the top surface of the high-performance chromatograph (1) for containing a mobile phase, wherein the right side of the mobile phase solution bottle (2) is connected to an external pipe (3), characterized in that: A sample bottle assembly (5) is provided on the right side of the high-efficiency chromatograph (1); a clamping assembly (4) for clamping the sample bottle assembly (5) is provided at the middle of the right side wall of the high-efficiency chromatograph (1); and a bottom support assembly (6) for supporting the sample bottle assembly (5) is provided on the right side wall of the high-efficiency chromatograph (1) below the clamping assembly (4); The clamping assembly (4) comprises a fixed block (41) fixed to the right side wall of the high-efficiency chromatograph (1), a movable structure (42) arranged on the right side of the fixed block (41), four groups of inclined rods (43) symmetrically arranged on the front and rear sides of the movable structure (42), and two clamping claws (44) arranged at the right ends of the corresponding inclined rods (43), the movable structure (42) comprises a movable block (421) and two connecting plates (422) symmetrically arranged on the front and rear sides of the movable block (421), the two groups of inclined rods (43) on the same side are distributed in parallel up and down, the inclined rods (43) in the same group are arranged in parallel front and back, the inner inclined rod (43) is located to the left relative to the outer inclined rod (43), and the left end of the inclined rod (43) is adjacent to the movable block (421). The fixed block (41) is hinged, and the right end is fixedly connected to the left end of the clamp (44); the clamp (44) is tilted inwardly; one end of the connecting plate (422) is hinged to the movable block (421), and the other end extends between the two corresponding groups of inclined rods (43) on the same side and is hinged to the two groups of inclined rods (43) on the inner side; the left side wall of the movable block (421) is symmetrically provided with movable cylinders (423); the right side wall of the fixed block (41) is provided with a sliding cavity corresponding to the movable cylinder (423); an insertion rod (415) is fixed to the inner wall of the left side of the sliding cavity; a spring (416) is provided on the outer sleeve of the insertion rod (415); and the insertion rod (415) is inserted into the corresponding movable cylinder (423); A spring plate (424) is fixed in the middle of the left side wall of the movable block (421), a clamping rod (425) is fixed to the bottom surface of the spring plate (424) near the front end, a spring plate groove corresponding to the position and size of the spring plate (424) is provided in the middle of the fixed block (41), a straight groove (411) is provided on the right side of the bottom surface of the spring plate groove, a first curved groove (412) is provided on the left end of the straight groove (411) near the rear side, and a second curved groove (414) is provided on the front side, the first curved groove (412) and the second curved groove (414) are in a rhombus shape The left end of the protruding portion between the first curved groove (412) and the second curved groove (414) is provided with an arc-shaped bayonet (417); the bottom surface of the first curved groove (412) gradually increases in height from the right end to the left end; the bottom surface of the second curved groove (414) gradually decreases in height from the right end to the left end; the bottom surface of the first curved groove (412) smoothly transitions with the straight groove (411); and a cushion block (413) is fixed to the middle of the left side wall where the left ends of the first curved groove (412) and the second curved groove (414) meet.

2. The device for detecting tauroursodeoxycholic acid in freeze-dried bear bile according to claim 1, characterized in that: The sample bottle assembly (5) comprises a bottle body (51) and a connecting tube (52) fixed in the middle of the bottle body (51); the top end of the connecting tube (52) is connected to the external tube (3) via a hose; a control valve is provided in the connecting tube (52) near the top; a first piston (53) is provided in the bottle body (51); the connecting tube (52) passes through the first piston (53) and the top surface of the bottle body (51) from bottom to top; the first piston (53) is slidably connected to the connecting tube (52); a plurality of openings (521) are provided at the bottom end of the connecting tube (52); and a liquid inlet is provided on the side wall of the bottle body (51) near the bottom.

3. The device for detecting tauroursodeoxycholic acid in freeze-dried bear bile according to claim 2, characterized in that: An air cavity (511) is provided in the middle of the bottom surface of the bottle body (51), a second piston (55) with a matching size is provided in the air cavity (511), limiting rods (514) are symmetrically provided in the air cavity (511), the limiting rods (514) pass through the second piston (55) and the two are slidably connected, and air flow channels (54) are symmetrically provided in the side wall of the bottle body (51).

4. The device for detecting tauroursodeoxycholic acid in freeze-dried bear bile according to claim 3, characterized in that: The top end of the air flow channel (54) is in communication with the top of the bottle body (51), and the bottom end is in communication with the top of the air cavity (511). An annular plate (512) is provided on the inner wall of the bottle body (51) below the top of the air flow channel (54). The lowest height of the first piston (53) is higher than the opening (521) and the highest height is lower than the annular plate (512). The highest height of the second piston (55) is lower than the height of the bottom end of the air flow channel (54).

5. The device for detecting tauroursodeoxycholic acid in freeze-dried bear bile according to claim 4, characterized in that: The base support assembly (6) comprises a base (61) and an extrusion structure (62) arranged in the base (61) for extruding a sample solution into the high-performance chromatograph (1); a bottle groove (611) is arranged in the middle of the top surface of the base (61) in left and right horizontal directions; the bottom of the bottle body (51) is matched with the bottle groove (611); an active cavity (614) is arranged in the base (61) below the bottle groove (611); and the extrusion structure (62) is arranged in the active cavity (614).

6. The device for detecting tauroursodeoxycholic acid in freeze-dried bear bile according to claim 5, characterized in that: The extrusion structure (62) comprises a mounting seat (621), a worm gear (623) arranged above the mounting seat (621), and a threaded rod (624) coaxially connected to the top of the worm gear (623); left and right lateral sliding rods (615) are arranged near the bottom of the movable cavity (614); the sliding rods (615) pass through the mounting seat (621) and the two are slidably connected; the middle part of the bottom surface of the worm gear (623) is embedded in the top surface of the mounting seat (621) and the worm gear (623) can rotate freely; a meshing worm (626) is arranged on the left side of the worm gear (623); a knob (627) is coaxially connected to the front end of the rotating shaft of the worm gear (626); support rods (622) are symmetrically fixed to the side wall of the mounting seat (621) in the front and rear directions; the rotating shaft of the worm gear (626) passes through the support rod (622) and the rotating shaft of the worm gear (626) can rotate.

7. The device for detecting tauroursodeoxycholic acid in freeze-dried bear bile according to claim 6, characterized in that: The threaded rod (624) is provided with a push rod (625) on its outer sleeve. The threaded rod (624) is threadedly connected to the middle of the push rod (625). The push rod (625) is provided with a flange on its side wall. The bottom surface of the air cavity (511) is provided with a notch (513) that matches the position and size of the flange. A push rod groove (613) that matches the push rod (625) is provided between the bottle groove (611) and the movable cavity (614). The movable cavity (614) is provided with a shaft groove (612) that matches the rotating shaft of the worm (626).

8. A method for detecting tauroursodeoxycholic acid in freeze-dried bear bile, using the device for detecting tauroursodeoxycholic acid in freeze-dried bear bile according to any one of claims 1 to 7, characterized in that: The following steps are involved: S1, dissolving the bear bile freeze-dried product in an ammonium chloride aqueous solution in proportion, injecting the solution into the bottle body (51) from the liquid inlet after it is completely dissolved, and connecting the connecting tube (52) and the external tube (3) through a hose; S2, aligning the notch (513) at the bottom of the bottle body (51) with the flange of the push rod (625) and placing the bottle body (51) into the bottle groove (611), and then pushing the bottle body (51) to the left to squeeze the movable block (421), and the movable block (421) moving to the left pulls the inclined rods (43) on both sides to rotate inward through the connecting plate (422), thereby driving the clamping claws (44) to clamp the bottle body (51); S3, the movable block (421) moves to the left and drives the clamping rod (425) to enter the first curved groove (412) along the straight groove (411), and enters the clamping hole (417) under the guidance of the cushion block (413), and the user can release the clamping rod at this time; S4. During the test, the tester first opens the control valve of the connecting pipe (52), then turns the knob (627), and drives the threaded rod (624) to rotate through the worm (626) and the worm gear (623), so that the push rod (625) pushes the second piston (55) upward, and the gas in the gas flow channel (54) pushes the first piston (53) downward, so that the sample solution is pressed from the opening (521) into the connecting pipe (52) and finally enters the external pipe (3) from the hose. At the same time, the pump of the mobile phase solution bottle (2) is started to bring the mobile phase and the sample solution into the high performance chromatograph (1) for testing; S5. After one determination, the mobile phase solution is reintroduced to clean the chromatographic column in the high performance chromatograph (1), and then the knob (627) is rotated again to perform a second determination of tauroursodeoxycholic acid. Repeating the above step S4 can perform multiple determinations of the sample.

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