Composition containing emamectin benzoate and application thereof

Abstract

The invention relates to the technical field of agrochemistry, in particular to a composition containing emamectin benzoate and application of the composition. The composition comprises methylamino abamectin benzoate, an anti-decomposition agent, an anti-ultraviolet agent, an emulsifier, a penetrant and a solvent. The micro-emulsion of the composition provided by the invention is small in particle size and good in permeability, so that the bioavailability of the emamectin benzoate is remarkably improved. Under different storage conditions, the microemulsion shows good physical and chemical stability. The microemulsion has low toxicity to non-target organisms, and meets the development requirements of green agriculture. The microemulsion provided by the invention can effectively improve the application effect of the emamectin benzoate, and has wide market prospects and practical values.

Description

Technical Field

[0001] The present invention relates to the technical field of agrochemicals, and in particular to a composition comprising emamectin benzoate and application thereof. Background Art

[0002] Emamectin benzoate is a broad-spectrum insecticide mainly used to control a variety of pests. It affects the nervous system and interferes with the transmission of nerve signals, causing paralysis and death of insects. It has a good control effect on many pests, especially Lepidoptera and Hemiptera pests. It is relatively safe for humans, animals and the environment and has low toxicity. This pesticide is widely used in agricultural production to increase crop yield and quality.

[0003] Emamectin benzoate can bind to the nerve cell membranes of insects, interfere with the transmission of nerve signals, and cause dysfunction of the nervous system. The compound can enhance the opening of GABA (γ-aminobutyric acid)-dependent chloride ion channels, leading to an increase in intracellular chloride ion concentrations, which in turn triggers excessive inhibition of nerve cells, leading to paralysis and death of insects. By affecting the transmission of nerve signals, it causes disordered muscle contraction in insects, resulting in loss of motor ability. The physical properties of emamectin benzoate include its appearance as a white or light yellow solid, which is easily soluble in organic solvents but has a low solubility in water. Its melting point is approximately between 150-160 degrees Celsius and has high stability. In addition, the compound is relatively sensitive to light and heat and needs to be properly stored to maintain its activity.

[0004] Microemulsion is a transparent or translucent liquid dispersion system, which consists of an aqueous phase, an oil phase and a surfactant. Microemulsions usually show high stability under temperature, light and storage conditions, and are not easy to stratify or phase separate. The particle size is generally between 10 and 100 nanometers, and can form a transparent or translucent liquid; it can form a stable emulsion between the oil and water phases and increase the uniformity of the liquid. Saponins are a class of natural glycoside compounds that are widely found in plants. They are composed of a sugar part and a non-sugar part (usually a saponin base or a steroid), and have foam-forming and surface-active properties.

[0005] In view of the above situation, the present invention provides a composition comprising avermectin benzoate and application thereof. Summary of the invention

[0006] In order to solve the above technical problems, the present invention provides a composition comprising emamectin benzoate, wherein the composition comprises: emamectin benzoate, an antidecomposition agent, an anti-ultraviolet agent, an emulsifier, a penetrant and a solvent.

[0007] As an embodiment of the present invention, the anti-decomposition agent is 2,6-di-tert-butyl-4-methylphenol or butylated hydroxyanisole.

[0008] As an embodiment of the present invention, the anti-ultraviolet agent is UV-531.

[0009] As an embodiment of the present invention, the emulsifier is calcium dodecylbenzene sulfonate, branched sodium dodecyl diphenyl ether disulfonate and isomeric decanol polyoxyethylene polyoxypropylene ether.

[0010] As an embodiment of the present invention, the permeation agent comprises sodium diisooctyl sulfosuccinate, saponin and mannitol.

[0011] As an embodiment of the present invention, the solvent is diethylene glycol butyl ether, isobutanol, and water.

[0012] As an embodiment of the present invention, the composition comprises, in parts by mass:

[0013] Emamectin benzoate 2.0

[0014] Antidecomposition agent 0.3~1.5

[0015] Anti-ultraviolet agent 0.1~5

[0016] Emulsifier 8~15

[0017] Penetrant 2~5

[0018] The solvent was made up to 100 parts.

[0019] As an embodiment of the present invention, the composition comprises, in parts by mass:

[0020] Emamectin benzoate 2.0

[0021] Antidegradant 0.5

[0022] Anti-ultraviolet agent 0.3

[0023] Emulsifier 10.8

[0024] Penetrant 4

[0025] The solvent was made up to 100 parts.

[0026] As an embodiment of the present invention, the dosage form of the composition is a microemulsion.

[0027] As an embodiment of the present invention, the composition is used in the field of insecticide.

[0028] By adopting the above technical solution, the present invention has the following beneficial effects:

[0029] The fine particle size and good permeability of the composition microemulsion provided by the present invention significantly improve the bioavailability of emamectin benzoate. Under different storage conditions, the microemulsion exhibits good physical and chemical stability. The microemulsion has low toxicity to non-target organisms and meets the development requirements of green agriculture. The microemulsion of the present invention can effectively improve the application effect of emamectin benzoate and has broad market prospects and practical value. DETAILED DESCRIPTION

[0030] The technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] The present invention is further explained below in conjunction with specific implementation modes.

[0032] Example 1

[0033] This embodiment provides a microemulsion containing avermectin benzoate, and the raw materials for its preparation include:

[0034] Emamectin benzoate 2.0 parts

[0035] 0.3 parts of 2,6-di-tert-butyl-4-methylphenol

[0036] 0.2 parts of butylated hydroxyanisole

[0037] UV-531 0.3 parts

[0038] 0.8 parts of calcium dodecylbenzene sulfonate

[0039] 3.0 parts of branched sodium dodecyl diphenyl ether disulfonate

[0040] 3.0 parts of sodium diisooctyl sulfosuccinate

[0041] Ginsenoside 0.5 parts

[0042] Mannitol 0.5 parts

[0043] 7.0 parts of isomeric decanol polyoxyethylene polyoxypropylene ether

[0044] 5.0 parts of diethylene glycol butyl ether

[0045] 5.0 parts of isobutyl alcohol

[0046] Water replenishment

[0047] The preparation method of the microemulsion described in the present invention is:

[0048] Mix avermectin benzoate, 2,6-di-tert-butyl-4-methylphenol, butyl hydroxyanisole, UV-531, diethylene glycol butyl ether and isobutyl alcohol evenly, add branched sodium dodecyl diphenyl ether disulfonate, stir evenly, then add calcium dodecylbenzene sulfonate, sodium diisooctyl sulfosuccinate, isomerized decanol polyoxyethylene polyoxypropylene ether and continue to stir evenly, then add the remaining raw materials and continue to stir evenly.

[0049] Example 2

[0050] The difference between Example 2 and Example 1 is that this example provides a microemulsion containing avermectin benzoate, and the raw materials for its preparation include:

[0051] Emamectin benzoate 2.0 parts

[0052] 0.3 parts of 2,6-di-tert-butyl-4-methylphenol

[0053] 0.2 parts of butylated hydroxyanisole

[0054] UV-531 0.3 parts

[0055] 0.8 parts of calcium dodecylbenzene sulfonate

[0056] 3.0 parts of branched sodium dodecyl diphenyl ether disulfonate

[0057] 4.0 parts of sodium diisooctyl sulfosuccinate

[0058] 7.0 parts of isomeric decanol polyoxyethylene polyoxypropylene ether

[0059] 5.0 parts of diethylene glycol butyl ether

[0060] 5.0 parts of isobutyl alcohol

[0061] Water replenishment

[0062] The preparation method of the microemulsion described in the present invention is:

[0063] Mix avermectin benzoate, 2,6-di-tert-butyl-4-methylphenol, butyl hydroxyanisole, UV-531, diethylene glycol butyl ether and isobutyl alcohol evenly, add branched sodium dodecyl diphenyl ether disulfonate, stir evenly, then add calcium dodecylbenzene sulfonate, sodium diisooctyl sulfosuccinate, isomerized decanol polyoxyethylene polyoxypropylene ether and continue to stir evenly, then add the remaining raw materials and continue to stir evenly.

[0064] Example 3

[0065] The difference from Example 1 is that this example provides a microemulsion containing avermectin benzoate, and the raw materials for its preparation include:

[0066] Emamectin benzoate 2.0 parts

[0067] 0.3 parts of 2,6-di-tert-butyl-4-methylphenol

[0068] 0.2 parts of butylated hydroxyanisole

[0069] UV-531 0.3 parts

[0070] 0.8 parts of calcium dodecylbenzene sulfonate

[0071] 3.0 parts of sodium diisooctyl sulfosuccinate

[0072] Ginsenoside 0.5 parts

[0073] Mannitol 0.5 parts

[0074] 10.0 parts of isomeric deca-alcohol polyoxyethylene polyoxypropylene ether

[0075] 5.0 parts of diethylene glycol butyl ether

[0076] 5.0 parts of isobutyl alcohol

[0077] Water replenishment

[0078] Example 4

[0079] The difference from Example 1 is that this example provides a microemulsion containing avermectin benzoate, and the raw materials for its preparation include:

[0080] Emamectin benzoate 2.0 parts

[0081] 0.3 parts of 2,6-di-tert-butyl-4-methylphenol

[0082] 0.2 parts of butylated hydroxyanisole

[0083] UV-531 0.3 parts

[0084] 0.8 parts of calcium dodecylbenzene sulfonate

[0085] 3.0 parts of branched sodium dodecyl diphenyl ether disulfonate

[0086] 3.5 parts of sodium diisooctyl sulfosuccinate

[0087] Mannitol 0.5 parts

[0088] 7.0 parts of isomeric decanol polyoxyethylene polyoxypropylene ether

[0089] 5.0 parts of diethylene glycol butyl ether

[0090] 5.0 parts of isobutyl alcohol

[0091] Water replenishment

[0092] Example 5

[0093] The difference from Example 1 is that this example provides a microemulsion containing avermectin benzoate, and the raw materials for its preparation include:

[0094] Emamectin benzoate 2.0 parts

[0095] 0.3 parts of 2,6-di-tert-butyl-4-methylphenol

[0096] 0.2 parts of butylated hydroxyanisole

[0097] UV-531 0.3 parts

[0098] 0.8 parts of calcium dodecylbenzene sulfonate

[0099] 10.0 parts of branched sodium dodecyl diphenyl ether disulfonate

[0100] 3.0 parts of sodium diisooctyl sulfosuccinate

[0101] Ginsenoside 0.5 parts

[0102] Mannitol 0.5 parts

[0103] 5.0 parts of diethylene glycol butyl ether

[0104] 5.0 parts of isobutyl alcohol

[0105] Water replenishment

[0106] Example 6

[0107] The difference from Example 1 is that this example provides a microemulsion containing avermectin benzoate, and the raw materials for its preparation include:

[0108] Emamectin benzoate 2.0 parts

[0109] 0.3 parts of 2,6-di-tert-butyl-4-methylphenol

[0110] 0.2 parts of butylated hydroxyanisole

[0111] UV-531 0.3 parts

[0112] 0.8 parts of calcium dodecylbenzene sulfonate

[0113] 3.0 parts of branched sodium dodecyl diphenyl ether disulfonate

[0114] 3.0 parts of sodium diisooctyl sulfosuccinate

[0115] Ginsenoside 0.5 parts

[0116] Mannitol 0.5 parts

[0117] 7.0 parts of isomeric decanol polyoxyethylene polyoxypropylene ether

[0118] Isobutanol 10.0 parts

[0119] Water replenishment

[0120] Performance Testing

[0121] 1. Determination of stability of microemulsions

[0122] Emulsion stability test: According to GB / T 1603-2001. In a 250mL beaker, add 100mL (30±2)℃ standard hard water, use a pipette to draw 0.5mL of the sample, slowly add it into the hard water (diluted 200 times) while stirring continuously to make 100mL of emulsion; stir at a speed of 2-3r / s for 30s, immediately transfer the emulsion to a clean and dry 100mL measuring cylinder, and place the measuring cylinder in a constant temperature water bath, within the range of (30±2)℃, and let it stand for 1h; take out the measuring cylinder and observe the separation of the emulsion. If there is no floating oil (cream) and precipitation in the measuring cylinder, the emulsion stability is determined to be qualified.

[0123] Thermal storage stability test: Conducted in accordance with GB / T 19136-2003. Use a syringe to inject about 30 mL of the sample into a clean glass bottle (avoid the sample from contacting the bottleneck), place the glass bottle in a constant temperature box at (54±2)℃ for 14 days, take out the glass bottle after 14 days, and use high performance liquid chromatography to determine the thermal storage decomposition rate of emamectin benzoate within 24 hours.

[0124] Low temperature stability test: Conduct according to GB / T 19137-2003. Take 100mL of sample and place it in a centrifuge tube, cool it to (0±2)℃ in a refrigerator, keep the centrifuge tube and its contents at (0±2)℃ for 1h, and stir it every 15min for 15s each time, check and record whether there is solid or oily precipitation. Put the centrifuge tube back into the refrigerator and continue to place it at (0±2)℃ for 7d; after 7d, take out the centrifuge tube, let it stand at room temperature (not exceeding 20℃) for 3h, centrifuge it for 15min, and record the volume of the precipitate at the bottom of the tube (accurate to 0.05mL).

[0125] Table 1 Performance test results

[0126]

[0127]

[0128] 2. Field efficacy experiment

[0129] Field test on the efficacy of emamectin benzoate microemulsion in controlling Plutella xylostella on cruciferous vegetables

[0130] Test agent: Emamectin benzoate microemulsion of Examples 1, 2, 4, and 6.

[0131] Application method and water consumption (L / hectare): Spray method is used, with a water consumption of 50 kg per mu.

[0132] Dosage of active ingredient: 4 ml / hectare (prepared dosage); spray CK with an equal amount of clean water.

[0133] Selection of test subjects, crops and varieties

[0134] Cruciferous vegetables Diamondback moth Plutella xylostella (L.) Lepidoptera, Diamondback moth family. Also known as diamondback moth, square moth, small green caterpillar, two-headed moth. Synonym Plutella maculipennis curtis. Mainly damages cruciferous vegetables such as cabbage, cauliflower, Chinese cabbage, rapeseed, and radish.

[0135] The vegetable variety tested was cauliflower (Longfeng Extra Large), which was in the early rosette stage of growth, with about 2,500 plants planted per mu.

[0136] The experiment was set up in Xiuling Village, Gushan Town, Jin'an District, Fuzhou City, at an altitude of about 20 meters. Plots with relatively consistent crop growth, similar insect population density, same agricultural cultivation management, and similar soil were selected.

[0137] Plot area: 20 square meters per plot; number of repetitions: 4 times.

[0138] Use Linong HD-400 sprayer to spray the leaves evenly. Use ground film as a barrier to prevent droplets from drifting when applying pesticides.

[0139] A 5-point sampling method was used, with 3 plants sampled at each point, i.e. 15 plants were checked in each plot; fixed-point and fixed-plant surveys were conducted.

[0140] Calculation formula:

[0141]

[0142] Table 2 Test results of microemulsion for controlling Plutella xylostella on cruciferous vegetables

[0143]

[0144]

[0145] The DMRT method was used for the significant difference test.

[0146] The present invention found that when preparing the emamectin benzoate microemulsion, adding branched sodium dodecyl diphenyl ether disulfonate, saponin, and mannitol can obtain better stability and insecticidal effect. This is because the existing formula preparation focuses on using surfactant properties to achieve the stability of the microemulsion, film-forming property and permeability after spraying; the inventor found that in order to improve the stability of the microemulsion, the permeability is often poor. The possible reason is that the components on the leaf surface itself and the effective ingredients are in a similar miscible relationship, and although the addition of surfactants can reduce surface tension, it also increases the integration of other components and the "loss" of the leaf surface. For this reason, the inventor added plant ingredient saponin during preparation to achieve better film-forming property and also improve some insecticidal effects. However, the stability often needs to be adjusted, so adding branched sodium dodecyl diphenyl ether disulfonate and isomeric decanol polyoxyethylene polyoxypropylene ether can obtain better effects.

[0147] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A composition comprising emamectin benzoate, characterized in that: The composition comprises: emamectin benzoate, an anti-decomposition agent, an anti-ultraviolet agent, an emulsifier, a penetrant and a solvent.

2. A composition comprising emamectin benzoate according to claim 1, characterized in that: The anti-decomposition agent is 2,6-di-tert-butyl-4-methylphenol and butylated hydroxyanisole.

3. A composition comprising emamectin benzoate according to claim 1, characterized in that: The anti-ultraviolet agent is UV-531.

4. A composition comprising emamectin benzoate according to claim 1, characterized in that: The emulsifier is calcium dodecylbenzene sulfonate, branched sodium dodecyl diphenyl ether disulfonate and isomeric decanol polyoxyethylene polyoxypropylene ether.

5. A composition comprising emamectin benzoate according to claim 1, characterized in that: The penetrant comprises sodium diisooctyl sulfosuccinate, saponin and mannitol.

6. A composition comprising emamectin benzoate according to claim 1, characterized in that: The solvent is diethylene glycol butyl ether, isobutyl alcohol and water.

7. A composition comprising emamectin benzoate according to claim 1, characterized in that: In parts by mass, the composition comprises: Emamectin benzoate 2.0 Antidecomposition agent 0.3~1.5 Anti-ultraviolet agent 0.1~5 Emulsifier 8~15 Penetrant 2~5 The solvent was made up to 100 parts.

8. A composition comprising emamectin benzoate according to claim 1, characterized in that: In parts by mass, the composition comprises: Emamectin benzoate 2.0 Antidegradant 0.5 Anti-ultraviolet agent 0.3 Emulsifier 10.8 Penetrant 4 The solvent was made up to 100 parts.

9. A composition comprising emamectin benzoate according to any one of claims 1 to 8, characterized in that: Its dosage form is microemulsion.

10. A composition comprising emamectin benzoate according to any one of claims 1 to 8, characterized in that: It is used in the field of insecticide.

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