Electrostatic spinning mask with whitening and tightening effects and preparation method thereof

Abstract

The invention discloses an electrostatic spinning mask with whitening and tightening effects and a preparation method of the electrostatic spinning mask. The electrostatic spinning mask comprises a nanofiber membrane formed by glutathione and collagen through an electrostatic spinning technology. In a spinning solution for preparing the nanofiber membrane through an electrostatic spinning technology, the mass fraction of glutathione is 5-20wt%, the mass fraction of collagen is 30-75wt%, and the balance is water. The nanofiber membrane is subjected to electrostatic spinning through electrostatic spinning equipment, and a spinning solution is poured into a spinning solution tank of the electrostatic spinning equipment; a collecting mechanism of the electrostatic spinning equipment accumulates and forms the filaments, and the nanofiber membrane is obtained after vacuum drying. The limitation of a traditional non-woven fabric mask can be overcome, and the skin care effect and the use experience are improved. According to the mask, the electrostatic spinning technology is adopted, the nanoscale fiber membrane is directly prepared without adding other chemical auxiliaries, the structure of the nanoscale fiber membrane is highly similar to a skin extracellular matrix, and health and regeneration of skin cells are promoted.

Description

Technical Field

[0001] This invention belongs to the field of facial mask preparation technology, and relates to a facial mask, and more particularly to an electrostatic spun facial mask with whitening and firming effects and its preparation method. Background Technology

[0002] Facial masks, as a skincare product with a long history, can be traced back to ancient Egypt. Over time, the materials and techniques used to make facial masks have become increasingly diverse and developed, evolving from the initial mixtures of natural substances to the later application of chemical agents and biotechnology. The types and functions of facial masks have also continuously expanded.

[0003] Non-woven fabric masks are the most common type of mask on the market. Using non-woven fabric as a carrier, they adhere to the facial skin and effectively deliver the nutrients in the mask. However, non-woven fabric masks have some limitations: First, their breathability is relatively limited, and prolonged use may cause skin discomfort due to heat and stuffiness. Second, their fit is problematic, especially when the essence content is insufficient; the edges of the mask tend to lift, affecting complete contact between the skin and the mask, thus reducing the absorption efficiency of the essence. Furthermore, chemical additives that may be introduced during the manufacturing process pose a potential risk to some users with sensitive skin, causing allergies or discomfort. Finally, the ease of use of non-woven fabric masks, which are based on essence, needs improvement; the ease with which the essence drips and other inconveniences diminish the enjoyment and relaxation that consumers should experience when using skincare products.

[0004] In view of this, there is an urgent need to design a new face mask that can overcome at least some of the aforementioned defects of existing face masks. Summary of the Invention

[0005] This invention provides an electrostatic spinning facial mask with whitening and firming effects and its preparation method, which can improve skin care effects and user experience.

[0006] To solve the above-mentioned technical problems, according to one aspect of the present invention, the following technical solution is adopted:

[0007] An electrospun facial mask with whitening and firming effects, the electrospun facial mask comprising: a nanofiber membrane formed by electrospun technology using glutathione and collagen.

[0008] In one embodiment of the present invention, the spinning solution for preparing nanofiber membranes by electrospinning technology contains 5-20 wt% glutathione, 30-75 wt% collagen, and the remainder is water.

[0009] In one embodiment of the present invention, the nanofiber membrane is electrospun using an electrospinning device, and the spinning solution is poured into the spinning solution tank of the electrospinning device; the collecting mechanism of the electrospinning device accumulates the fibers into shape, and after vacuum drying, a nanofiber membrane is obtained, which serves as the electrospinned facial mask with whitening and firming effects.

[0010] In one embodiment of the present invention, the ratio of glutathione to collagen protein in the nanofiber membrane is (5-20):(30-75).

[0011] In one embodiment of the present invention, the ratio of glutathione to collagen protein in the nanofiber membrane is (5-15):(60-70).

[0012] In one embodiment of the present invention, the collagen is a recombinant human collagen obtained by bio-fermentation technology, with an average molecular weight between 500 Da and 4000 Da.

[0013] In one embodiment of the present invention, the average molecular weight of the collagen is between 1000 Da and 2500 Da.

[0014] According to another aspect of the present invention, the following technical solution is adopted: a method for preparing an electrostatically spun facial mask with whitening and firming effects, the preparation method comprising:

[0015] S1. Spinning solution preparation steps;

[0016] Glutathione and collagen were added to deionized water and stirred thoroughly until completely dissolved. The homogenized solution was then used to obtain the spinning solution.

[0017] S2, Electrospinning step;

[0018] The above spinning solution is poured into the spinning solution tank of the electrospinning equipment for electrospinning. The fibers are collected and shaped by the collection mechanism of the electrospinning equipment, and after vacuum drying, a nanofiber membrane is obtained, which can be used as an electrospinning mask with whitening and firming effects.

[0019] In one embodiment of the present invention, in step S1, the spinning solution includes the following components in weight percentage: glutathione 5-20 wt% and collagen 30-75 wt%.

[0020] The parameters were set as follows: temperature 30-40℃, stirring speed 1000-3500rpm for 2-4 hours, and homogenization at 2500-3500rpm for 10-15 minutes.

[0021] In one embodiment of the present invention, in step S2, the parameters of the electrospinning process are set as follows: voltage 15-30kV, receiving distance between needle and platform is 5-20cm, pusher speed is 0.5-5mL / h, and roller speed is 200-300rpm.

[0022] The parameters for the vacuum drying process are set as follows: vacuum degree 0.01-0.1MPa, temperature 20-30℃, and time 2-4h.

[0023] The beneficial effects of this invention are as follows: The electrostatic spinning mask with whitening and firming effects proposed in this invention and its preparation method can overcome the limitations of traditional non-woven masks and improve the skin care effect and user experience.

[0024] The mask of this invention uses electrospinning technology to directly prepare nanoscale fiber membranes without the need for additional chemical additives. Its structure is highly similar to the extracellular matrix of skin cells, promoting skin cell health and regeneration.

[0025] Glutathione and collagen are the core ingredients in the mask. Glutathione effectively whitens the skin by anti-oxidation and inhibiting melanin synthesis, while collagen helps to firm the skin and fight aging with its excellent water retention, support and elasticity properties.

[0026] The nanofiber structure of electrospun masks further enhances the penetration efficiency of ingredients, allowing glutathione and collagen to penetrate the stratum corneum more quickly and reach the dermis, thus significantly enhancing skincare effects. Simultaneously, its high specific surface area, excellent breathability, and uniform release properties ensure comprehensive coverage and sustained action of skincare ingredients, avoiding the uneven distribution of ingredients that may occur in traditional masks.

[0027] Furthermore, masks made using electrospinning technology achieve a high degree of adhesion to the skin surface thanks to their lightweight design and soft touch. When a dry mask is applied to pre-moistened skin, the mask material responds quickly to the moisture released by the skin, completing hydration and reconstruction. This process significantly improves wearing comfort while effectively eliminating air gaps and wrinkles, ensuring a continuous and uninterrupted contact interface between the mask and the skin.

[0028] Maximizing this contact interface not only enhances the direct interaction between active skincare ingredients and the stratum corneum, but also promotes efficient penetration and absorption of the ingredients. By reducing obstacles to the diffusion path, it improves the uniformity of distribution and cumulative concentration of skincare ingredients in skin tissue, thereby significantly optimizing skincare effects. Attached Figure Description

[0029] Figure 1 This is a flowchart of a method for preparing an electrostatically spun facial mask with whitening and firming effects according to an embodiment of the present invention. Detailed Implementation

[0030] The preferred embodiments of the present invention will now be described in detail with reference to the accompanying drawings.

[0031] To further understand the present invention, preferred embodiments of the present invention are described below in conjunction with examples. However, it should be understood that these descriptions are only for further illustrating the features and advantages of the present invention, and not for limiting the scope of the claims of the present invention.

[0032] The description in this section pertains to only a few typical embodiments, and the present invention is not limited to the scope of the embodiments described. Substitution of identical or similar prior art methods with some technical features in the embodiments is also within the scope of the description and protection of this invention.

[0033] Unless otherwise stated, implied from the context, or as is customary in the art, all parts and percentages in this application are based on weight, and all testing and characterization methods used are concurrent with the filing date of this application. Where applicable, any patent, patent application, or disclosure relating to this application is incorporated herein by reference in its entirety, and its equivalent patent families are also incorporated herein by reference, particularly the definitions disclosed in these documents concerning synthetic techniques, product and processing design, polymers, comonomers, initiators, or catalysts in the art. If any definition of a specific term disclosed in the prior art is inconsistent with any definition provided in this application, the definition provided in this application shall prevail.

[0034] The numerical ranges in this application are approximate values ​​and therefore may include values ​​outside the range unless otherwise stated. A numerical range includes all values ​​from the lower limit to the upper limit, increasing by one unit, provided there is an interval of at least two units between any lower and any higher value. For example, if a component, physical, or other property (such as molecular weight, melt index, etc.) is described as 100 to 1000, this means that all individual values, such as 100, 101, 102, etc., are explicitly listed, as well as all subranges, such as 100 to 166, 155 to 170, 198 to 200, etc. For ranges containing values ​​less than 1 or fractions greater than 1 (e.g., 1.1, 1.5, etc.), one unit is appropriately considered as 0.0001, 0.001, 0.01, or 0.1. For ranges containing single digits less than 10 (e.g., 1 to 5), one unit is generally considered as 0.1. These are merely specific examples of what is intended to be expressed, and all possible combinations of values ​​between the listed minimum and maximum values ​​are considered to be clearly stated in this application. It should also be noted that the terms "first," "second," etc., used herein are not intended to specify a particular order, but are merely used to distinguish substances with different structures.

[0035] When referring to chemical compounds, unless explicitly stated otherwise, the singular includes all isomers and vice versa (e.g., "hexane" includes all isomers of hexane, individually or collectively). Additionally, unless explicitly stated otherwise, nouns described with "an," "a," or "the" also include their plural forms.

[0036] The terms “comprising,” “including,” “having,” and their derivatives do not exclude the presence of any other components, steps, or processes, regardless of whether such other components, steps, or processes are disclosed in this application. To eliminate any doubt, unless expressly stated otherwise, all compositions using the terms “comprising,” “including,” or “having” in this application may contain any additional additives, excipients, or compounds. Conversely, except for those necessary for operational performance, the term “substantially constitutes…” excludes any other components, steps, or processes described below with respect to that term. The term “consisting of…” does not include any components, steps, or processes not specifically described or listed. Unless expressly stated otherwise, the term “or” refers to the individual members listed or any combination thereof.

[0037] The steps described in the various embodiments in the specification are for illustrative purposes only, and the implementation of this application is not limited by the order of the steps.

[0038] This invention discloses an electrospun facial mask with whitening and firming effects, the electrospun facial mask comprising: a nanofiber membrane formed by electrospun technology using glutathione and collagen.

[0039] In one embodiment of the present invention, the spinning solution for preparing the nanofiber membrane by electrospinning technology contains 5-20 wt% glutathione, 30-75 wt% collagen, and the remainder is water. The nanofiber membrane is electrospun using an electrospinning device. The spinning solution is poured into the spinning solution tank of the electrospinning device; the collecting mechanism of the electrospinning device accumulates the fibers into a shape, and after vacuum drying, a nanofiber membrane is obtained, which serves as the electrospinned facial mask with whitening and firming effects.

[0040] In one embodiment, the ratio of glutathione to collagen protein in the nanofiber membrane is (5-20):(30-75). Further, the ratio of glutathione to collagen protein in the nanofiber membrane can be (5-15):(60-70).

[0041] The collagen is a recombinant human collagen obtained through bio-fermentation technology, with an average molecular weight between 500 Da and 4000 Da. Further, the average molecular weight of the collagen is between 1000 Da and 2500 Da.

[0042] This invention further discloses a method for preparing an electrostatically spun facial mask with whitening and firming effects. Figure 1 This is a flowchart illustrating a method for preparing an electrostatically spun facial mask with whitening and firming effects according to an embodiment of the present invention; please refer to [link / reference]. Figure 1 The preparation method includes:

[0043]

Step S1

[0044] Add glutathione and collagen to deionized water (if possible, add them sequentially), stir thoroughly until completely dissolved, and homogenize to obtain the spinning solution;

[0045] In one embodiment, the spinning solution comprises the following components by weight percentage: 5-20 wt% glutathione and 30-75 wt% collagen;

[0046] The parameters were set as follows: temperature 30-40℃, stirring at 1000-3500 rpm for 2-4 hours, and then homogenizing at 2500-3500 rpm for 10-15 minutes.

[0047]

Step S2

[0048] The above spinning solution is poured into the spinning solution tank of the electrospinning equipment for electrospinning. The fibers are collected and shaped by the collection mechanism of the electrospinning equipment, and after vacuum drying, a nanofiber membrane is obtained, which can be used as an electrospinning mask with whitening and firming effects.

[0049] In one embodiment, the parameters for the electrospinning process are set as follows: voltage 15-30kV, receiving distance between needle and platform 5-20cm, feeder speed 0.5-5mL / h, and roller speed 200-300rpm; the parameters for the vacuum drying process are set as follows: vacuum degree 0.01-0.1MPa, temperature 20-30℃, and time 2-4h.

[0050] Glutathione is a potent antioxidant. Its thiol (-SH) group directly participates in free radical scavenging reactions. Through single-electron transfer or addition reactions, it reacts with reactive oxygen species (ROS) such as superoxide anion (O2-), hydrogen peroxide (H2O2), and hydroxyl radical (·OH), effectively inhibiting the chain reaction of oxidative stress and protecting cells from oxidative damage.

[0051] This antioxidant defense mechanism maintains the redox balance of skin cells, reduces DNA, protein, and lipid damage caused by oxidative stress, thereby slowing down the skin aging process and inhibiting the melanin synthesis pathway triggered by oxidative stress.

[0052] Furthermore, glutathione inhibits the tyrosinase-catalyzed conversion of tyrosine to dopa by directly binding to the active site of tyrosinase (TYR) or regulating the expression of its upstream regulatory factors, thereby blocking the initial steps of melanin synthesis and controlling the formation of skin pigmentation at its source. In addition, the antioxidant effect of GSH indirectly reduces the stimulation of TYR activity by oxidative stress.

[0053] Furthermore, glutathione promotes the metabolism and excretion of melanin and its precursors by upregulating the expression or activity of melanin degradation-related enzymes; it also affects the transport and distribution of melanin granules within skin cells, accelerating their movement to the upper epidermis and eventual shedding with the stratum corneum. This mechanism accelerates melanin metabolism and clearance, helping to reduce existing age spots and dullness.

[0054] Furthermore, glutathione regulates the distribution of melanin types by affecting the activity or expression level of specific enzymes in the melanin synthesis pathway, such as promoting the conversion of eumelanin synthases (e.g., TYR, DOPAchrome tautomerase, etc.) to pheomelanin synthases (e.g., TRP-1, TRP-2, etc.), thereby making the skin tone brighter and more even.

[0055] Collagen is characterized by its unique glycine-XY (GlyXY) tripeptide repeat sequence, where X and Y are mostly proline (Pro) and hydroxyproline (Hyp). Collagen's unique triple helix structure is stabilized through intramolecular and intermolecular interactions such as hydrogen bonds and van der Waals forces, forming strong mechanical strength and elastic modulus, providing structural integrity and toughness for connective tissue.

[0056] Collagen is one of the main components of the extracellular matrix (ECM), and together with elastic fibers, hyaluronic acid, etc., it forms the "foundation" of the skin. This foundation provides a place for skin cells to grow and metabolize, and also provides the skin with good mechanical properties and biocompatibility.

[0057] Collagen and elastin fibers together form the skin's elastic network. When the skin is subjected to external forces, elastin fibers can undergo elastic deformation and return to their original shape, while collagen provides the necessary support and stability, enabling the skin to maintain good elasticity and resilience.

[0058] In addition, collagen molecules contain a large number of hydrophilic groups (such as hydroxyl and carboxyl groups), which can form hydrogen bonds with water molecules, thereby absorbing and locking in surrounding moisture. This water-retaining property helps maintain the skin's hydration, making the skin look smoother and plumper.

[0059] Collagen also participates in the formation of the skin's natural barrier system, which can block the invasion of harmful external substances and protect the skin from damage. At the same time, it can also promote the normal metabolism and repair of skin cells and enhance the skin's resistance.

[0060] Furthermore, collagen intertwines into a network structure in the dermis of the skin, providing a solid support framework. This structural framework not only helps maintain the firmness and contour of the skin, but also ensures the stability and elasticity of the skin when subjected to external forces.

[0061] Electrospinning is an advanced nanomaterial preparation technology that atomizes polymer solutions or melts into tiny jets under a high-voltage electric field. These jets are then stretched, refined, bent, and solidified to form nanofiber membranes on a receiving device. This technology boasts advantages such as fine fiber diameter, large specific surface area, high porosity, and controllable structure, showing broad application prospects in various fields. However, its application in the skincare industry remains largely unexplored.

[0062] Based on this, the present invention introduces electrospinning technology into the design and manufacturing of facial mask products. The fiber membrane prepared by electrospinning technology can reach the nanometer scale in diameter, which is highly similar to the natural fiber structure of the skin cell extracellular matrix. This similarity not only promotes the affinity between the mask and the skin surface, but also helps to simulate the skin microenvironment, providing skin cells with support and protection that is closer to their physiological state, thus promoting cell health and regeneration.

[0063] The finer fiber structure gives electrospun masks an extremely high specific surface area, increasing the effective contact area between the mask and the skin. This structural advantage significantly improves the penetration efficiency of active ingredients such as glutathione and collagen, allowing these ingredients to penetrate the stratum corneum more quickly and reach the dermis, thereby enhancing skincare effects.

[0064] The microporous network formed between the electrospun fibers in the mask creates highly efficient breathable channels, ensuring that the skin can maintain normal gas exchange during application. This breathability helps reduce moisture buildup on the skin surface, lowers the risk of bacterial growth, and enhances user comfort.

[0065] The fine fiber network of electrostatic spinning masks can act as a uniform carrier for skin care ingredients, ensuring that various skin care ingredients can be released evenly and stably to the skin surface during application. This avoids the problem of uneven ingredient distribution that may occur in traditional masks, and guarantees the comprehensiveness and consistency of skin care effects.

[0066] Electrostatically spun facial masks, with their lightweight and soft properties, can closely conform to the contours of the face, reducing air gaps and wrinkles. This high degree of fit not only improves the comfort of wearing the mask but also helps to increase the contact area between skincare ingredients and the skin, thereby enhancing skincare effects.

[0067] Example 1

[0068] In this embodiment, the preparation method of the electrostatic spun facial mask with whitening and firming effects of the present invention includes the following steps:

[0069] (1) Preparation of spinning solution

[0070] Mix 10g of glutathione, 70g of collagen and 20g of deionized water, heat to 30℃, stir at 3000rpm for 3h, and homogenize at 3000rpm for 10min to obtain spinning solution.

[0071] (2) Electrospinning

[0072] The prepared spinning solution is poured into the spinning solution tank for electrospinning, and the fibers are collected and shaped on the collecting mechanism.

[0073] The parameters for the electrospinning process are: voltage 25kV, receiving distance between needle and platform 18cm, feeder speed 1.5mL / h, and roller speed 200rpm.

[0074] The parameters for the vacuum drying process are: vacuum degree 0.05MPa, temperature 25℃, and time 4h.

[0075] Example 2

[0076] In this embodiment, the preparation method of the electrostatic spun facial mask with whitening and firming effects of the present invention includes the following steps:

[0077] (1) Preparation of spinning solution

[0078] Mix 15g of glutathione, 75g of collagen and 10g of deionized water, heat to 30℃, stir at 3000rpm for 3h, and homogenize at 3000rpm for 10min to obtain spinning solution.

[0079] (2) Electrospinning

[0080] The prepared spinning solution is poured into the spinning solution tank for electrospinning, and the fibers are collected and shaped on the collecting mechanism.

[0081] The parameters for the electrospinning process are: voltage 25kV, receiving distance between needle and platform 18cm, feeder speed 1.5mL / h, and roller speed 200rpm.

[0082] The parameters for the vacuum drying process are: vacuum degree 0.05MPa, temperature 25℃, and time 4h.

[0083] Example 3

[0084] In this embodiment, the preparation method of the electrostatic spun facial mask with whitening and firming effects of the present invention includes the following steps:

[0085] (1) Preparation of spinning solution

[0086] Mix 5g of glutathione, 65g of collagen and 30g of deionized water, heat to 30℃, stir at 3000rpm for 3h, and homogenize at 3000rpm for 10min to obtain spinning solution.

[0087] (2) Electrospinning

[0088] The prepared spinning solution is poured into the spinning solution tank for electrospinning, and the fibers are collected and shaped on the collecting mechanism.

[0089] The parameters for the electrospinning process are: voltage 25kV, receiving distance between needle and platform 18cm, feeder speed 1.5mL / h, and roller speed 200rpm.

[0090] The parameters for the vacuum drying process are: vacuum degree 0.05MPa, temperature 25℃, and time 4h.

[0091] The difference between the comparative example and the embodiment is that the comparative example uses nonwoven fabric as the receiving substrate for electrospinning.

[0092] Comparative Example 1

[0093] In this comparative example, the mask preparation method includes the following steps:

[0094] (1) Preparation of spinning solution

[0095] Mix 10g of glutathione, 70g of collagen and 20g of deionized water, heat to 30℃, stir at 3000rpm for 3h, and homogenize at 3000rpm for 10min to obtain spinning solution.

[0096] (2) Electrospinning

[0097] The prepared spinning solution is poured into the spinning solution tank, and electrospinning is performed using nonwoven fabric as the receiving substrate.

[0098] The parameters for the electrospinning process are as follows: voltage 25kV, receiving distance between needle and platform 18cm, feeder speed 1.5mL / h, and roller speed 200rpm.

[0099] The parameters for the vacuum drying process are: vacuum degree 0.05MPa, temperature 25℃, and time 4h.

[0100] Comparative Example 2

[0101] In this comparative example, the mask preparation method includes the following steps:

[0102] (1) Preparation of spinning solution

[0103] Mix 15g of glutathione, 75g of collagen and 10g of deionized water, heat to 30℃, stir at 3000rpm for 3h, and homogenize at 3000rpm for 10min to obtain spinning solution.

[0104] (2) Electrospinning

[0105] The prepared spinning solution is poured into the spinning solution tank, and electrospinning is performed using nonwoven fabric as the receiving substrate.

[0106] The parameters for the electrospinning process are as follows: voltage 25kV, receiving distance between needle and platform 18cm, feeder speed 1.5mL / h, and roller speed 200rpm.

[0107] The parameters for the vacuum drying process are: vacuum degree 0.05MPa, temperature 25℃, and time 4h.

[0108] Comparative Example 3

[0109] In this comparative example, the mask preparation method includes the following steps:

[0110] (1) Preparation of spinning solution

[0111] Mix 5g of glutathione, 65g of collagen and 30g of deionized water, heat to 30℃, stir at 3000rpm for 3h, and homogenize at 3000rpm for 10min to obtain spinning solution.

[0112] (2) Electrospinning

[0113] The prepared spinning solution is poured into the spinning solution tank, and electrospinning is performed using nonwoven fabric as the receiving substrate.

[0114] The parameters for the electrospinning process are: voltage 25kV, receiving distance between needle and platform 18cm, feeder speed 1.5mL / h, and roller speed 200rpm.

[0115] The parameters for the vacuum drying process are: vacuum degree 0.05MPa, temperature 25℃, and time 4h.

[0116] Blank example 1

[0117] In this blank example, the mask preparation method includes the following steps:

[0118] (1) Preparation of spinning solution

[0119] Mix 80g of collagen and 20g of deionized water, heat to 30℃, stir at 3000rpm for 3h, and homogenize at 3000rpm for 10min to obtain the spinning solution.

[0120] (2) Electrospinning

[0121] The prepared spinning solution is poured into the spinning solution tank, and electrospinning is performed using nonwoven fabric as the receiving substrate.

[0122] The parameters for the electrospinning process are: voltage 25kV, receiving distance between needle and platform 18cm, feeder speed 1.5mL / h, and roller speed 200rpm.

[0123] The parameters for the vacuum drying process are: vacuum degree 0.05MPa, temperature 25℃, and time 4h.

[0124] Blank example 2

[0125] In this blank example, the mask preparation method includes the following steps:

[0126] (1) Preparation of spinning solution

[0127] Mix 30g of glutathione and 70g of deionized water, heat to 30℃, stir at 3000rpm for 3h, and homogenize at 3000rpm for 10min to obtain the spinning solution.

[0128] (2) Electrospinning

[0129] The prepared spinning solution is poured into the spinning solution tank, and electrospinning is performed using nonwoven fabric as the receiving substrate.

[0130] The parameters for the electrospinning process are as follows: voltage 25kV, receiving distance between needle and platform 18cm, feeder speed 1.5mL / h, and roller speed 200rpm.

[0131] The parameters for the vacuum drying process are: vacuum degree 0.05MPa, temperature 25℃, and time 4h.

[0132] Experimental results show that the solution in this scheme has low viscosity and poor molding, and is not suitable for single-component electrospinning. Therefore, it is not included in the subsequent determination of product characterization data.

[0133] Characterization data and effect data of the products in the examples and comparative examples

[0134] 1. Security Testing

[0135] 1) Test sample: spinning solution from Examples 1-3.

[0136] 2) Number of participants: 120, half male and half female, aged between 22 and 45 years old, all of whom met the criteria for participants.

[0137] 3) Testing Method: 120 participants meeting the subject criteria were randomly divided into 4 groups. The experimental group received the textile liquid from Examples 1-3, while the control group received no sample. Each group consisted of 30 participants. Safety was tested by a third-party testing agency. Areas not exceeding 50 mm² were selected. 2 A suitable patch applicator with a depth of approximately 1 mm was used. Approximately 0.02-0.025 ml (g) of the test substance was added to the applicator using a closed patch test method. The applicator was applied to the flexor side of the subject's forearm. After 24 hours, the test substance was removed, and skin reactions were observed at 0.5 h, 24 h, and 48 h after removal. The results were recorded according to the skin reaction grading standards in the "Cosmetic Safety Technical Regulations 2015".

[0138] Table 1 Results of the patch experiment

[0139]

[0140]

[0141] Note: Skin reaction grading standards:

[0142] Grade 0: Negative reaction;

[0143] Grade 1: Suspicious reaction; only slight erythema;

[0144] Grade 2: Weak positive reaction (erythema reaction); erythema, infiltration, edema, and papules may be present;

[0145] Grade 3: Strong positive reaction (herpes reaction); erythema, infiltration, edema, papules, herpes: the reaction may extend beyond the tested area;

[0146] Grade 4: Extremely strong reaction (confluent herpes simplex reaction): obvious erythema, severe infiltration, edema, confluent herpes simplex, reaction extending beyond the test area.

[0147] 4) Test results: The results in Table 1 show no irritation, indicating that the test passed safely.

[0148] 2. Stability Test

[0149] 1) Test sample: textile liquid from Examples 1-3.

[0150] 2) Test method: Internal control standard, observe appearance, color, viscosity and pH for three months.

[0151] Table 2. Results of Stability Study

[0152] time 45℃ 40℃ 37℃ 25℃ 5℃ -15℃ CYCLE illumination 1W √ √ √ √ √ √ √ √ 2W √ √ √ √ √ √ √ √ 3W √ √ √ √ √ √ √ √ 4W √ √ √ √ √ √ √ √ 2M √ √ √ √ √ √ √ √ 3M √ √ √ √ √ √ √ √

[0153] "CYCLE" refers to a cycle, which is set to heat from -15℃ to 45℃ and then cool down from 45℃ to -15℃ five times.

[0154] 4) Test results: Table 2 shows that the textile liquids in Examples 1-3 are stable in properties within 3 months.

[0155] Efficacy evaluation test

[0156] 1) Test samples: Examples 1-3 electrospun masks, Comparative Examples 1-3 electrospun masks, and Blank Example 1 electrospun mask.

[0157] 2) Test subjects: 70 healthy women (aged 20-50, mean age 38.7 years) were randomly divided into 70 groups of 10 each, regardless of gender. Each group used the same formula product. Written informed consent was obtained. Before enrollment, subjects were asked a series of questions regarding their medical history and health status according to the inclusion and exclusion criteria. Simultaneously, a conformity assessment and skin color test were performed on the test sites, and the results were recorded.

[0158] Environmental conditions: The visual assessment and instrument testing were conducted in an environment with a temperature of 21±1℃ and a relative humidity of 50±10%RH. The visual assessment was conducted under constant lighting conditions (fluorescent tubes or LED lights with a color temperature of 5500~6500K). Subjects were required to adapt to these environmental conditions for at least 30 minutes before assessment and testing could be performed.

[0159] 3) Test method: The samples were used every evening. The electrospun masks of Examples 1-3, Comparative Examples 1-3 and Blank Example 1 were applied to the face. After 15 minutes, the masks were removed without washing. The trial lasted for 4 weeks. Subjects applied sunscreen to their entire face every morning.

[0160] Results were tested at the following three time periods: before sample use (D0), 14 days after sample use (D14), and 28 days after sample use (D28).

[0161] 4) The testing items are:

[0162] ①The Cutometer MPA580 is used for skin tightening sampling;

[0163] ②After acquiring full-face photos of the subjects using Visia-CR, Image-Pro Plus was used to analyze the proportion of pigmented spots and the mean optical density of pigmented spots within the AOI area;

[0164] ③The Cutometer MPA580 is used for skin elasticity testing;

[0165] ④ The G lossymeter GL200 is used for skin gloss measurement.

[0166] 5) The test results can be calculated using the following formula:

[0167] Change rate = (Average analytical value of 10 people in each group after product use - Average analytical value of 10 people in each group before product use) ÷ Average analytical value of 10 people in each group before product use × 100%

[0168] 6) Test Results

[0169] ①The Cutometer MPA580 is used to collect skin tightness data. The larger the skin tightness measurement value and the greater the rate of change, the better the skin tightness.

[0170] Table 3. Skin Firmness Change Rate

[0171]

[0172]

[0173] As shown in Table 3, after using the electrospun mask, the rate of change in skin firmness values ​​of Examples 1-3, Comparative Examples 1-3, and Blank Example 1 all increased with prolonged use, indicating that skin firmness was improved. However, compared with Comparative Examples 1-3, the rate of change in skin firmness values ​​of Examples 1-3 was greater for the same period of use, indicating that the improvement in skin firmness after using the electrospun mask of Examples 1-3 was more significant.

[0174] Comparison of the results of Examples 1-3, Comparative Examples 1-3 and Blank Example 1 shows that, compared with the masks that use non-woven fabric as the receiving substrate for electrospinning, the masks that directly use glutathione and collagen for electrospinning have a better effect on improving skin firmness.

[0175] ②After collecting full-face photos of the subjects using Visia-CR, Image-Pro Plus was used to analyze the proportion of pigmented area and the mean of pigmented light density within the AOI region. The smaller the proportion of pigmented area, the fewer the spots, and the smaller the rate of change, indicating that the more spots are reduced and the better the whitening effect.

[0176] Table 4. Change rate of the proportion of pigmented area

[0177] sample % after 14 days of use % after 28 days of use Example 1 -7.46 -10.63 Example 2 -8.73 -12.18 Example 3 -7.01 -9.15 Comparative Example 1 -4.37 -6.49 Comparative Example 2 -4.93 -7.23 Comparative Example 3 -3.24 -5.41 Blank example 1 -0.85 -1.12

[0178] As shown in Table 4, after using the electrospun mask, the rate of change in the proportion of pigmented area in Examples 1-3 and Comparative Examples 1-3 decreased with the extension of usage time, indicating that the proportion of pigmented area was improved. Blank Example 1 showed no significant change.

[0179] However, compared with Comparative Examples 1-3, the rate of change in the proportion of pigmented area in Examples 1-3 was smaller when used for the same period of time, indicating that the improvement effect on the proportion of pigmented area after using the electrospun mask of Examples 1-3 was more significant.

[0180] Comparison of the results of Examples 1-3, Comparative Examples 1-3 and Blank Example 1 shows that, compared with the mask that uses non-woven fabric as the receiving substrate for electrospinning, the mask that directly uses glutathione and collagen for electrospinning has a better effect on improving the proportion of pigmentation area and a better whitening effect.

[0181] ③ Skin elasticity. Elasticity is evaluated using the test parameters of the Cutometer MPA580. The R2 value is the ratio of the skin's rebound without negative pressure to its maximum stretch value with negative pressure. The greater the rate of change of the ratio, the better the elasticity.

[0182] Table 5. Changes in Skin Elasticity Rate

[0183] sample % after 14 days of use % after 28 days of use Example 1 5.79 7.94 Example 2 6.83 8.42 Example 3 5.35 7.16 Comparative Example 1 3.89 4.75 Comparative Example 2 4.27 5.18 Comparative Example 3 3.23 4.26 Blank example 1 1.41 3.22

[0184] As shown in Table 5, after using the electrospun facial mask, the rate of change in skin elasticity of Examples 1-3, Comparative Examples 1-3 and Blank Example 1 all increased with the extension of the usage time, indicating that the skin elasticity was improved.

[0185] However, compared with Comparative Examples 1-3 and Blank Example 1, the skin elasticity change rate of Examples 1-3 was greater when used for the same period of time, indicating that the improvement effect on skin elasticity after using the electrospun mask of Examples 1-3 was more significant.

[0186] A comparison of the results of Examples 1-3, Comparative Examples 1-3, and Blank Example 1 shows that, compared with masks that use non-woven fabric as the receiving substrate for electrospinning, masks that directly use glutathione and collagen for electrospinning have a better effect on improving skin elasticity.

[0187] ④ Skin glossiness is measured using a GL200 ossymeter. The higher the measured value and the greater the rate of change, the better the skin glossiness.

[0188] Table 6. Change Rate of Skin Glossiness

[0189] sample % after 14 days of use % after 28 days of use Example 1 13.23 22.75 Example 2 15.16 24.13 Example 3 12.58 21.06 Comparative Example 1 9.05 15.82 Comparative Example 2 9.67 16.16 Comparative Example 3 8.24 14.94 Blank example 1 5.72 8.93

[0190] As shown in Table 6, after using the electrospun mask, the rate of change in skin gloss of Examples 1-3, Comparative Examples 1-3 and Blank Example 1 all increased with the extension of the usage time, indicating that the skin gloss was improved.

[0191] However, compared with Comparative Examples 1-3 and Blank Example 1, the skin gloss change rate of Examples 1-3 was greater when used for the same period of time, indicating that the improvement effect on skin gloss after using the electrospun mask of Examples 1-3 was more significant.

[0192] A comparison of the results of Examples 1-3, Comparative Examples 1-3, and Blank Example 1 shows that, compared with masks that use non-woven fabric as the receiving substrate for electrospinning, masks that directly use glutathione and collagen for electrospinning have a better effect on improving skin radiance.

[0193] In summary, the electrostatic spun facial mask with whitening and firming effects and its preparation method proposed in this invention can overcome the limitations of traditional non-woven facial masks and improve skin care effects and user experience.

[0194] The mask of this invention uses electrospinning technology to directly prepare nanoscale fiber membranes without the need for additional chemical additives. Its structure is highly similar to the extracellular matrix of skin cells, promoting skin cell health and regeneration.

[0195] Glutathione and collagen are the core ingredients in the mask. Glutathione effectively whitens the skin by anti-oxidation and inhibiting melanin synthesis, while collagen helps to firm the skin and fight aging with its excellent water retention, support and elasticity properties.

[0196] The nanofiber structure of electrospun masks further enhances the penetration efficiency of ingredients, allowing glutathione and collagen to penetrate the stratum corneum more quickly and reach the dermis, thus significantly enhancing skincare effects. Simultaneously, its high specific surface area, excellent breathability, and uniform release properties ensure comprehensive coverage and sustained action of skincare ingredients, avoiding the uneven distribution of ingredients that may occur in traditional masks.

[0197] Furthermore, masks made using electrospinning technology achieve a high degree of adhesion to the skin surface thanks to their lightweight design and soft touch. When a dry mask is applied to pre-moistened skin, the mask material responds quickly to the moisture released by the skin, completing hydration and reconstruction. This process significantly improves wearing comfort while effectively eliminating air gaps and wrinkles, ensuring a continuous and uninterrupted contact interface between the mask and the skin.

[0198] Maximizing this contact interface not only enhances the direct interaction between active skincare ingredients and the stratum corneum, but also promotes efficient penetration and absorption of the ingredients. By reducing obstacles to the diffusion path, it improves the uniformity of distribution and cumulative concentration of skincare ingredients in skin tissue, thereby significantly optimizing skincare effects.

[0199] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0200] The description and application of the present invention herein are illustrative and not intended to limit the scope of the invention to the embodiments described above. Effects or advantages involved in the embodiments may not be apparent due to various factors, and the description of effects or advantages is not intended to limit the embodiments. Variations and modifications of the embodiments disclosed herein are possible, and various substitutions and equivalents of the components in the embodiments are well known to those skilled in the art. It should be apparent to those skilled in the art that the invention can be implemented in other forms, structures, arrangements, proportions, and with other components, materials, and parts without departing from the spirit or essential characteristics of the invention. Other variations and modifications can be made to the embodiments disclosed herein without departing from the scope and spirit of the invention.

Claims

1. An electrospinning facial mask with whitening and firming effects, characterized in that: The electrostatic spinning mask comprises a nanofiber membrane formed by electrostatic spinning technology using glutathione and collagen.

2. The electrospinning facial mask with whitening and firming effects according to claim 1, characterized in that: In the spinning solution for preparing the nanofiber membrane by electrostatic spinning technology, the mass fraction of glutathione is 5-20wt%, the mass fraction of collagen is 30-75wt%, and the rest is water.

3. The electrospinning facial mask with whitening and firming effects according to claim 1, characterized in that: The nanofiber membrane is electrospun by an electrospinning device, and the spinning solution is poured into the spinning solution tank of the electrospinning device; the collecting mechanism of the electrospinning device accumulates and shapes the fibers, and the nanofiber membrane is obtained after vacuum drying, which serves as the electrospinning mask with whitening and firming effects.

4. The electrospinning facial mask with whitening and firming effects according to claim 1, characterized in that: The mass ratio of glutathione and collagen in the nanofiber membrane is (5-20): (30-75).

5. The electrospinning facial mask with whitening and firming effects according to claim 4, characterized in that: The mass ratio of glutathione and collagen in the nanofiber membrane is (5-15):(60-70).

6. The electrospinning facial mask with whitening and firming effects according to claim 1, characterized in that: The collagen is a recombinant human collagen obtained through biological fermentation technology, and its average molecular weight is between 500Da and 4000Da.

7. The electrospinning facial mask with whitening and firming effects according to claim 6, characterized in that: The average molecular weight of the collagen is between 1000Da and 2500Da.

8. A method for preparing an electrospinning facial mask with whitening and firming effects, characterized in that: The preparation method comprises: Step S1, spinning solution preparation step; Add glutathione and collagen into deionized water, stir thoroughly until completely dissolved, and homogenize to obtain a spinning solution; Step S2, electrospinning step; The spinning solution is poured into the spinning solution tank of the electrospinning equipment for electrospinning. The fibers are accumulated and formed on the collecting mechanism of the electrospinning equipment. After vacuum drying, a nanofiber membrane is obtained as an electrospinning mask with whitening and firming effects.

9. The preparation method according to claim 8, characterized in that: In step S1, the spinning solution includes the following components in weight percentage: 5-20 wt% glutathione and 30-75 wt% collagen; The parameters were set as follows: temperature 30-40°C, rotation speed 1000-3500 rpm for stirring for 2-4 hours, and homogenization at 2500-3500 rpm for 10-15 minutes.

10. The preparation method according to claim 8, characterized in that: In step S2, the parameters of the electrospinning process are set as follows: voltage 15-30 kV, receiving distance between the needle and the platform 5-20 cm, propeller rate 0.5-5 mL / h, roller speed 200-300 rpm; The parameters of the vacuum drying process are set as follows: vacuum degree 0.01-0.1 MPa, temperature 20-30°C, and time 2-4 h.

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