Traditional Chinese medicine composition for preventing and treating myopia and application thereof

Abstract

The invention discloses a traditional Chinese medicine composition for preventing and treating myopia and application thereof. The traditional Chinese medicine composition is composed of a midday yang tonifying formula and a child-hour yin nourishing formula, and the effect of preventing and treating myopia is achieved by taking a yang medicine when yang is taken and taking a yin medicine when yin is taken according to the pathogenesis of liver and kidney deficiency and yin and yang disharmony of myopia, nourishing yin, tonifying yang and adjusting yin and yang balance. Meanwhile, the walnut, the Chinese chestnut, the pig spinal cord and other medicinal and edible medicinal materials are selected, nutrients are taken by food through a dietary therapy method, and the food is safe, free of obvious adverse side effects, beneficial to long-term taking and capable of reducing the risk of eye diseases.

Description

Technical Field

[0001] The invention relates to a Chinese medicine composition for preventing and treating myopia and application thereof, and in particular to a Chinese medicine composition for preventing and treating myopia which is both a medicine and a food and application thereof. Background Art

[0002] With the development of media technologies such as mobile phones, videos, and WeChat, people use artificial light sources at close range for a long time, which increases the burden on the eyes. The occurrence of myopia is becoming younger and more common, resulting in a high incidence of high myopia. Retinal detachment, retinal choroidal atrophy, and optic neuropathy caused by high myopia seriously damage vision.

[0003] At present, the methods for preventing and controlling myopia in children and adolescents include optical intervention, drug control and behavioral intervention. Optical intervention includes frame glasses and contact lenses. Single-focus frame glasses are mainly used to correct far vision, but cannot control the progression of myopia. The principle of bifocal or multifocal frame glasses to control myopia is to increase the sensitivity of accommodation, and the effect is significant only in the initial use. Corneal contact lenses (OK lenses) are a kind of lens that changes the shape of the anterior surface of the cornea to make the central cornea flat and the mid-peripheral cornea steep, so as to change the refractive power to achieve the purpose of correcting myopic refractive errors. However, the wearing requirements of corneal contact lenses are high. Improper wearing may cause complications such as infection and corneal damage, and rebound occurs after stopping wearing. The specific mechanism of atropine in drug control has not been fully clarified, and there is no recognized drug use standard. The process of atropine drops will produce side effects such as pupil dilation, photophobia and blurred near vision. Long-term use of atropine may cause side effects such as retinal light damage and concurrent cataracts. In addition, the effect of anticholinergic drugs such as atropine in controlling the progression of myopia will rebound, and the progression of myopia will accelerate after stopping the drug. Pirenzepine ophthalmic gel also has a certain control effect on myopia, but it may cause adverse reactions such as asymptomatic papillary conjunctivitis. Behavioral interventions include increasing outdoor activities, reducing close-range eye use, and sitting in a correct posture. Studies have shown that increasing the outdoor exercise time of children and adolescents can reduce their myopia incidence to a certain extent. However, Chinese teenagers have great academic pressure and heavy learning tasks, and spend too much time using their eyes at close range, making it difficult for them to do enough outdoor activities every day.

[0004] The above methods have certain effects on myopia prevention and control, but they all have certain limitations or side effects. Therefore, it is of great significance to explore new methods to prevent and control myopia in children and adolescents. Summary of the invention

[0005] Purpose of the invention: The present invention aims to provide a Chinese medicine composition for preventing and treating myopia, which can match the corresponding properties and flavors according to the yin and yang properties of different times and its application.

[0006] Technical solution: The Chinese medicine composition for preventing and treating myopia of the present invention is composed of a noon yang-tonifying recipe and a midnight yin-nourishing recipe; by weight, the noon yang-tonifying recipe contains 5-15 parts of walnuts, 5-15 parts of chestnuts, 3-10 parts of donkey-hide gelatin, 5-15 parts of black beans, and 5-15 parts of mung beans; the midnight yin-nourishing recipe contains 20-50 parts of pig spinal cord, 10-20 parts of tortoise shells, 10-30 parts of wolfberries, 5-15 parts of chrysanthemums, 10-20 parts of lilies, 5-15 parts of white fungus, 5-15 parts of lotus seeds, and 10-30 parts of corn.

[0007] Among them, the weight ratio of walnuts, chestnuts, donkey-hide gelatin, black beans and mung beans in the noon yang-tonifying recipe is 1:1:1:1:1.

[0008] Preferably, by weight, the noon yang-tonifying recipe contains 10 parts of walnuts, 10 parts of chestnuts, 10 parts of donkey-hide gelatin, 10 parts of black beans, and 10 parts of mung beans.

[0009] Among them, the weight ratio of pig spinal cord, tortoise shell, wolfberry, chrysanthemum, lily, white fungus, lotus seeds and corn in the midnight nourishing yin recipe is 3:1:2:1:2:1:1:2.

[0010] Preferably, by weight, the midnight nourishing yin recipe comprises 30 parts of pig spinal cord, 10 parts of tortoise shell, 20 parts of wolfberry, 10 parts of chrysanthemum, 20 parts of lily, 10 parts of white fungus, 10 parts of lotus seeds, and 20 parts of corn.

[0011] Preferably, the weight ratio of walnut, chestnut, donkey-hide gelatin, black bean, mung bean, pig spinal cord, tortoise shell, wolfberry, chrysanthemum, lily, white fungus, lotus seed and corn in the traditional Chinese medicine composition for preventing and treating myopia of the present invention is 1:1:1:1:1:3:1:2:1:2:1:1:2.

[0012] Further preferably, the Chinese medicine composition for preventing and treating myopia of the present invention comprises, by weight, 10 parts of donkey-hide gelatin, 10 parts of walnuts, 10 parts of chestnuts, 10 parts of black beans, 10 parts of mung beans, 20 parts of wolfberries, 30 parts of pig spinal cords, 10 parts of chrysanthemums, 10 parts of white fungus, 10 parts of tortoise shells, 20 parts of lilies, 10 parts of lotus seeds, and 20 parts of corn.

[0013] It is recorded in "Ten Books of Dongyuan": "Those who can see near but not far have insufficient Yang Qi and excess Yin Qi, which is Qi deficiency and blood abundance. Excessive blood means excess Yin Fire." Wu Qian's "Essentials of Ophthalmology" says: The sky has the sun and the moon, just as a person has two eyes. The sun and the moon in the sky are the essence of the Yin and Yang of the sky; the two eyes of a person are the essence of the five internal organs and six bowels of a person. The mutual dependence and mutual restraint of Yin and Yang are the premise for maintaining the normal operation of the Qi and blood functions of the human body. If the Yang Qi is insufficient, the heart Yang will be weak, the heart Qi will be deficient, the blood vessels will be blocked, the eyes will lose their nourishment, and the divine light will not be able to travel far. Or it may be due to the deficiency of the essence and blood, the deficiency of the liver and kidney, and the deficiency of liver blood, which will cause the divine light to weaken and the brilliance to not reach far.

[0014] The present invention believes that "imbalance of yin and yang" is the basic pathogenesis of myopia. Treatment should be to reconcile the dynamic balance of yin and yang, so that the body's yin and yang are in harmony with nature and reach a state of yin and yang balance, then the qi is nourished and the blood is restrained, so the eyesight is clear and the spirit is bright.

[0015] The medicinal properties and effects of each Chinese medicinal material in the present invention are as follows:

[0016] Walnut: warm in nature, sweet in taste, enters the kidney, lung, and large intestine meridians, has the effects of tonifying the kidney and yang, tonifying the lung and astringing the lung, moistening the intestines and relieving constipation, and is used to treat kidney yang deficiency, soreness of waist and knees, impotence and spermatorrhea, asthenia and cough, dry intestines and constipation, etc. Walnuts are rich in nutrients such as protein, high-quality fat, amino acids, phospholipids, etc., which can improve brain function, delay cell aging, and enhance immunity.

[0017] Chestnut: warm in nature, sweet in taste, and enters the spleen, stomach, and kidney meridians. "Compendium of Materia Medica" records: "Chestnuts can treat kidney deficiency, weakness in waist and legs, can clear the kidney and replenish qi, and strengthen the intestines and stomach. The kidney governs defecation, and chestnuts can govern the kidney." Chestnuts can nourish the spleen and stomach, and strengthen the kidneys and tendons. It is suitable for chronic diarrhea caused by spleen and stomach deficiency, and soreness of waist and knees, lumbar paralysis, and frequent urination caused by kidney deficiency. Chestnuts contain vitamin C, calcium, phosphorus, and low molecular weight albumin, which can not only provide a good source of plant protein, but also help maintain the health of bones and teeth.

[0018] Donkey-hide gelatin: It is mild in nature, sweet in taste, and enters the lung, liver, and kidney meridians. It has the effects of nourishing blood, nourishing yin, moistening the lungs, and stopping bleeding. Donkey-hide gelatin is a product of blood and flesh, sweet and mild in nature, and is an important blood tonic. It can treat blood deficiency and sallow complexion, dizziness and palpitations, muscle atrophy and weakness, restlessness and insomnia, internal wind movement, dry cough, hemoptysis due to fatigue, hematuria, bloody stool, metrorrhagia, and fetal bleeding during pregnancy. Donkey-hide gelatin is rich in a large amount of amino acids, polypeptides, and proteins, and can be antioxidant, anti-fatigue, blood-tonifying and blood-activating, anti-aging, and treat a variety of gynecological hemorrhagic diseases.

[0019] Black beans: neutral in nature, sweet in taste, and belong to the spleen and kidney meridians. They have the functions of nourishing essence and improving eyesight, nourishing blood and dispelling wind, replenishing deficiency and blackening hair, and promoting diuresis and detoxification. They are used to treat yin deficiency and thirst, dizziness and blurred vision, physical weakness and sweating, kidney deficiency and low back pain, edema and oliguria, pain and cramps, numbness of hands and feet, and drug and food poisoning. Black beans can strengthen the spleen and replenish qi to produce blood, and nourish kidney yin to transform blood. When kidney yin is sufficient, liver blood is nourished, menstrual blood is regulated, and eyes are bright. Black beans are rich in anthocyanins and isoflavones. Anthocyanins can reduce lipids, blood sugar, anti-inflammatory, and antibacterial. Isoflavones have anti-cancer and estrogen regulating effects.

[0020] Mung bean: cold in nature, sweet in taste, enters the heart and stomach meridians. It has the effects of clearing away heat and detoxifying, relieving heat and promoting diuresis. It is used for summer heat and thirst, erysipelas, carbuncle, edema, diarrhea, drug and food poisoning, etc. Mung bean skin can also reduce swelling, dissipate cataracts and improve eyesight. Mung bean contains a kind of globulin and polysaccharide, which can promote the decomposition of cholesterol in the liver and reduce the absorption of cholesterol by the small intestine, thus achieving the effect of lowering blood lipids and cholesterol.

[0021] Pig spinal cord: It is cold in nature, sweet in taste, and belongs to the kidney meridian. It has the effects of replenishing essence and marrow, nourishing yin and promoting muscle growth, and replenishing blood and marrow. It is used to treat kidney essence deficiency, bone steaming and fatigue fever, thirst, soreness of waist and knees, turbid spermatorrhea, ulcers, yin deficiency and internal heat, night sweats and other symptoms. Pig marrow is rich in nutrients such as protein, calcium, iron, zinc, acidic mucopolysaccharides and phospholipids, which can help the body replenish nutrition, promote bone growth and increase bone strength.

[0022] Turtle shell: It is cold in nature, sweet in taste, and enters the kidney, liver, and heart meridians. It has the effects of nourishing yin and suppressing yang, benefiting the kidney and strengthening bones, nourishing blood and nourishing the heart. It is used to treat dizziness, internal heat due to yin deficiency, night sweats and spermatorrhea caused by liver and kidney yin deficiency; weak tendons and bones, sore waist and knees, pigeon chest, turtle back, and fontanelle closure caused by kidney deficiency; palpitations, insomnia, forgetfulness and other symptoms caused by insufficient yin and blood, and malnutrition of the heart and kidney. It can also be used for metrorrhagia and menorrhagia caused by yin deficiency, internal heat, and Chong and Ren meridians being unstable.

[0023] Lycium barbarum: It is mild in nature, sweet in taste, and belongs to the liver and kidney meridians. It has the effects of nourishing the liver and kidney, improving the essence and improving the eyesight. It is used to treat vision loss, dry eyes, cataracts, weak waist and knees, spermatorrhea, deafness, insomnia, and liver and kidney yin deficiency, hot flashes, night sweats, and thirst caused by insufficient essence and blood. Lycium barbarum also has an immunomodulatory effect, which can increase the level of testosterone in the blood, enhance immune function, improve disease resistance, anti-oxidation, anti-aging, and relieve visual fatigue. Lycium barbarum is rich in lutein, zeaxanthin, and Lycium barbarum polysaccharides, which can inhibit the damage of active oxygen free radicals to optic nerve cells, and help prevent myopia, macular degeneration, glaucoma and other eye diseases.

[0024] Chrysanthemum: pungent, slightly cold, sweet, bitter, enters the lung and liver meridians, has the effects of dispersing wind-heat, calming liver yang, clearing away heat and detoxifying, and can be used to treat wind-heat colds, fever, headache, cough at the beginning of febrile diseases; dizziness caused by liver yang, red and swollen eyes caused by liver fire; sores, swelling and poison, etc. The flavonoids, chrysanthemum glycosides, adenine, chlorogenic acid, volatile oils and other substances in chrysanthemum have antioxidant, antibacterial, disinfectant, analgesic and other effects, and have a positive effect on the immune system, cardiovascular system and cholesterol metabolism.

[0025] Lily: cold in nature, sweet in taste, belongs to the heart meridian and lung meridian, has the effects of nourishing yin and moistening the lungs, clearing the heart and calming the mind, and is used to treat yin deficiency and dry cough, hemoptysis, restlessness and palpitations, insomnia, dreaminess, mental confusion, etc. Lily is rich in alkaloids, lily polysaccharides, lily glycosides and glycosides, which can increase blood cells, promote the mononuclear cell system and phagocytic system, and improve humoral immunity. Lily polysaccharides can repair pancreatic cells, enhance insulin secretion, and promote the combined effect of converting blood sugar into glycogen in the liver, thereby lowering blood sugar. Lily glycosides and glycosides have a sedative and hypnotic effect, which can significantly improve sleep quality and shorten the time to fall asleep.

[0026] Tremella: It is mild in nature, sweet in taste, and belongs to the lung, stomach, and kidney meridians. It has the effects of strengthening the essence and kidney, replenishing qi and blood, moistening the intestines and benefiting the stomach. It can be used to improve various symptoms such as consumptive cough, blood in sputum, lack of fluid and thirst, physical weakness after illness, etc. Tremella contains relatively rich polysaccharides and nutrients such as vitamin D and dietary fiber. The polysaccharides can improve the liver's detoxification ability and protect liver function. The dietary fiber in Tremella can also help gastrointestinal motility, accelerate metabolism, and improve constipation symptoms.

[0027] Lotus seeds: neutral in nature, sweet and astringent in taste, belonging to the spleen meridian, kidney meridian, and heart meridian. They have the effects of nourishing the kidney and consolidating essence, tonifying the spleen and stopping diarrhea, stopping leukorrhea, nourishing the heart and calming the mind. They can treat nocturnal emission, spermatorrhea, leukorrhea, spleen deficiency diarrhea, palpitations and insomnia. The dietary fiber in lotus seeds can optimize the dietary structure, and the glycoprotein can promote the reproduction and growth of bifidobacteria, improve the stress resistance of the gastric mucosa, and regulate gastrointestinal function.

[0028] Corn: It is cool in nature, sweet and salty in taste, and enters the kidney, spleen, and stomach meridians. It has the effects of harmonizing the middle and benefiting the kidney, clearing away heat and detoxifying. It can be used for people with spleen and stomach deficiency and heat, nausea and vomiting, abdominal distension and loss of appetite, thirst and diarrhea, etc. It can also be used as an external application to treat burns caused by water or fire. Corn is rich in carbohydrates, which can provide a stable source of energy for the body. The dietary fiber in corn helps increase intestinal peristalsis, improve digestive function, and relieve constipation.

[0029] The present invention proposes the concept of "Yin Yang Daily Clock Adjustment Method", taking corresponding prescriptions and medicines under the premise of complying with the law of meridian flow, regulating the disorder of biological rhythm, thereby correcting various diseases caused by rhythm disorder and achieving the effect of preventing disease. The "Yellow Emperor's Internal Classic" specifically discusses the nutrients needed by the human body: "Five grains for nourishment, five fruits for assistance, five animals for benefit, five vegetables for filling, take them with the right smell to replenish essence and benefit qi". Considering that it is not feasible for students in the window period to take Chinese medicine for a long time, and Chinese medicine "prevents disease" mostly uses "medicine and food of the same origin" formula, the present invention selects foods with corresponding properties and flavors and medicine and food of the same origin according to the Yin Yang properties of different times of the day, which are suitable for integration into daily diet plans, are more palatable and durable, and the micro RNA in the food is very stable, can smoothly enter the blood, and stay for a long time to play a regulatory role.

[0030] The kidney stores essence, opens up to the ears and two yin organs, and its beauty is in the hair. Sufficient kidney qi can be injected into the eyes to nourish the eyes. Therefore, warming yang and tonifying the kidney is particularly important for improving essence and improving eyesight. The walnuts used in the noon tonifying yang recipe are sweet and warm in nature. They can tonify the kidney, consolidate essence and strengthen the waist, warm the lungs and calm asthma, and moisten the intestines and promote bowel movements. Walnuts are considered the "brain fruit" and are rich in Omega-3 fatty acids, especially α-linolenic acid (ALA). They are not only beneficial to the cardiovascular and cerebrovascular systems, but also rich in antioxidants and melatonin, which help protect the retinal cells of the eyes from damage by free radicals. Chestnuts are sweet and warm in nature. They belong to the spleen, stomach, and kidney meridians and have the effects of strengthening the spleen and stomach, and strengthening the kidney and waist. Chestnuts are known as the "king of dried fruits" and are rich in carbohydrates, dietary fiber, carotene, and nutrients such as calcium, phosphorus, and potassium. Walnuts and chestnuts are paired together and taken in the morning when the yang energy rises in nature, which can better achieve the effects of warming yang and tonifying the kidney, strengthening the spleen and stomach. The black beans used in the recipe are also called "green kernel black beans". Green color enters the liver, and black color enters the kidneys, so they can nourish the liver and kidneys. Mung beans are rich in antioxidants and vitamin A, which help maintain the health of the retina and prevent night blindness.

[0031] "Compendium of Materia Medica" records that "the blood and flesh of animals are close to human blood and flesh, so they are more nourishing." The pig spinal cord and tortoise shell in the Zishi Yangyin Recipe are sentient blood and flesh, which can nourish the marrow sea and replenish the essence and benefit the marrow. Lycium barbarum nourishes the liver and kidney to improve eyesight, and chrysanthemum calms the liver, relieves wind and dissipates heat. The two medicines are compatible, and the liver yin is replenished, the liver fire is reduced, the liver qi is harmonious, and the vision is restored to normal. Tremella is flat in nature, sweet and light in taste, and enters the lung and stomach meridians, and has the effects of nourishing yin, moistening the lungs, nourishing the stomach, and promoting fluid production; lily is sweet and slightly bitter, slightly cold in nature, enters the heart and lung meridians, and has the effects of nourishing yin and moistening the lungs, clearing the heart and calming the mind. Lotus seeds are flat in nature, dry and astringent in taste, and enter the spleen, kidney, and heart meridians, and have the effects of nourishing the spleen and stopping diarrhea, benefiting the kidney and astringing the essence, and nourishing the heart and calming the mind. The five grains have the function of nourishing essence and kidney qi, but among the five grains, millet has the strongest kidney-tonifying effect, and is known as "substitute ginseng soup". It has the functions of nourishing spleen and stomach, nourishing kidney, nourishing heart and calming nerves, etc. The above ingredients can not only nourish yin and kidney, nourish spleen and moisten lungs, but also nourish heart and calm nerves when taken at night, which helps to promote sleep.

[0032] Zhang Jiebin said: "Those who are good at replenishing yang must seek yang in yin, so that yang can be helped by yin and its life will be endless; those who are good at replenishing yin must seek yin in yang, so that yin can be lifted by yang and its source will be inexhaustible." Therefore, donkey-hide gelatin is used in the yang-replenishing prescription, that is, it is used in the yang-replenishing prescription with yin-replenishing medicine, which means "seeking yang in yin", and wolfberry is used in the yin-replenishing prescription, that is, it is used in the yin-replenishing prescription with yang-replenishing medicine, which means "seeking yin in yang". The Rizhong diet therapy prescription harmonizes yin and yang by taking warming and replenishing yang products at noon and nourishing yin products at midnight, thereby correcting the development of myopia caused by the imbalance of yin and yang.

[0033] The Chinese medicinal composition for preventing and treating myopia of the present invention is used in preparing medicines for preventing and treating myopia in children and adolescents.

[0034] Preferably, the drug is a drug for preventing and treating mild myopia, moderate myopia, and severe myopia.

[0035] Preferably, in the Chinese medicine composition, the noon yang-tonifying prescription is taken at 7-12 am, and the midnight yin-nourishing prescription is taken at 17-21 pm.

[0036] Preferably, the drug can increase the dopamine content in the retina and reduce the retinoic acid content in the retina.

[0037] Preferably, the drug can down-regulate the expression levels of RAR-β and cRABP-I proteins and mRNA in the retina, and up-regulate the expression levels of DRD2 proteins and mRNA in the retina.

[0038] Beneficial effects: Compared with the prior art, the present invention has the following significant advantages:

[0039] The Chinese medicine composition designed by the present invention focuses on the pathogenesis of myopia "liver and kidney deficiency, imbalance of yin and yang", and achieves the effect of preventing and treating myopia by "taking yang medicine in yang time and taking yin medicine in yin time", nourishing yin and yang, regulating the balance of yin and yang. At the same time, the Chinese medicine composition is selected to form a prescription of medicinal materials with the same origin as medicine and food, and nutrients are taken in through food therapy, which is safe and has no obvious adverse side effects, is conducive to long-term use, and reduces the risk of eye diseases. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] Figure 1 The histological morphology of the guinea pig retina in each group (×400, scale bar = 50 μm);

[0041] Figure 2 These are the expression results of RAR-β, CRABP-I and DRD2 proteins in the retinal tissues of guinea pigs in each group. DETAILED DESCRIPTION

[0042] The technical solution of the present invention is further described below in conjunction with embodiments.

[0043] Example 1

[0044] Recipe: 10g donkey-hide gelatin, 10g walnut, 10g chestnut, 10g black bean, 10g mung bean; 30g pig spinal cord, 10g tortoise shell glue, 20g wolfberry, 10g chrysanthemum, 20g lily, 10g white fungus, 10g lotus seed, 20g corn.

[0045] The specific preparation method is as follows:

[0046] Soak the black beans and mung beans in the Yang-tonifying recipe and the lily, white fungus, lotus seeds, and corn in the Yin-nourishing recipe separately in water, with the amount of water exceeding the medicinal materials by 8 to 10 cm. Soak for 4 to 6 hours. Wash the walnuts, chestnuts, wolfberries, and chrysanthemums with water. Put the soaked black beans and mung beans into a pot, add water and boil over high heat, simmer for 20 minutes on low heat, turn to low heat and add walnuts and chestnuts until the black beans and mung beans are cooked, and the walnuts and chestnuts are cooked. Add donkey-hide gelatin and dissolve it fully. Take it between 7 and 12 noon. Boil fresh pig spinal cord and let it cool, dry naturally or at low temperature, crush and grind into powder for storage, add soaked lily, white fungus, lotus seeds, and corn in water and boil over high heat, then simmer for 20 minutes on low heat, add chrysanthemum, wolfberry, and pig spinal cord powder and simmer for 15 to 20 minutes on low heat, until lotus seeds and corn are cooked, add tortoise shell glue and dissolve it fully, then take it between 17:00 and 21:00 in the evening.

[0047] Example 2

[0048] Recipe: 10g donkey-hide gelatin, 10g walnut, 10g chestnut, 10g black bean, 10g mung bean; 30g pig spinal cord, 10g tortoise shell glue, 20g wolfberry, 10g chrysanthemum, 20g lily, 10g white fungus, 10g lotus seed, 20g corn.

[0049] Take the walnuts, chestnuts, black beans, and mung beans from the Yang-tonifying recipe and put them in a pot, stir-fry over low heat, serve and let cool, then crush and grind into powder, store in a cool and dry place, add about 20-30g of fine powder to a bowl each time you take it, add a small amount of warm water and let it stand, add the donkey-hide gelatin that has been stewed in water until it becomes viscous to a bowl, then add boiling water and stir to dissolve, and take it between 7 and 12 am. Cook the fresh pig spinal cord, let it cool, dry it naturally or dry it in a food dryer at low temperature, crush and grind it into powder, wash the wolfberry, chrysanthemum, lily, white fungus, lotus seeds, and corn, and use a food dryer to dry them to remove moisture. Crush the dried ingredients in batches several times, sieve the powder to remove larger particles and ensure the fineness of the powder, add pig spinal cord powder and mix, put into a sealed container, and store in a cool and dry place. Add about 20-30g of fine powder to a medium bowl each time, add a small amount of warm water and let it stand, add the tortoise shell glue that has been stewed in water until it becomes viscous into a bowl, then add boiling water and stir to dissolve, and take it between 17:00 and 21:00 in the evening

[0050] Example 3

[0051] Recipe: 10g donkey-hide gelatin, 10g walnut, 10g chestnut, 10g black bean, 10g mung bean; 30g pig spinal cord, 10g tortoise shell glue, 20g wolfberry, 10g chrysanthemum, 20g lily, 10g white fungus, 10g lotus seed, 20g corn.

[0052] Soak the walnut kernels, chestnuts, mung beans, and black beans in the Yang-tonifying recipe for at least 4 hours in advance until the ingredients become soft. Put the soaked mung beans and black beans into a pot, add appropriate amount of water, and cook until soft. After cooking, drain the water. Put the soaked chestnuts into a pot, add appropriate amount of water, and cook until soft and glutinous. After cooking, peel and mash them into a paste. Mix the cooked mung beans, black beans and mashed chestnut paste, add appropriate amount of brown sugar or white sugar, stir well, and make fillings. Beat the walnut kernels into coarse particles with a blender. Add appropriate amount of water to the donkey-hide gelatin and stew it in water until it becomes sticky, then add the walnut pieces and stir well. Take appropriate amount of glutinous rice flour, add a small amount of water, and knead into a ball. Divide the dough into several small portions, roll each portion into a round cake, and wrap it with appropriate amount of fillings and walnut kernel pieces. Knead the wrapped pastry into a round or square shape, put it into a mold and press it into shape. Put the formed pastry into a steamer and steam it over high heat for about 20 to 30 minutes until the glutinous rice flour is cooked. After steaming, let it cool slightly before serving. Take it between 7am and 12pm. Boil the pig spinal cord in the Yangyin recipe, let it cool, and chop it for later use. Wash and soak the wolfberry, wash and soak the chrysanthemum, lily, white fungus, and lotus seeds for 1 hour, put them in the pot and cook until soft and sticky. Steam the corn and mix it with the pig spinal cord, stir evenly, season with appropriate amount of salt or sugar, stir evenly, and make the filling. Add appropriate amount of hot water to the glutinous rice flour, knead it into a smooth glutinous rice ball, and divide it into several small portions. Take a portion of the glutinous rice ball, flatten it, wrap it with appropriate amount of fillings, close it and roll it into a ball. Put the wrapped cakes into the steamer and steam them on high heat for about 20 to 30 minutes until the glutinous rice balls are cooked. Take out the steamed cakes, brush a layer of tortoise shell glue on the surface to increase the nourishing effect, then put them on a plate and take them between 5pm and 9pm.

[0053] Example 4: Evaluation of eye axis and diopter

[0054] 1. Experimental methods

[0055] (1) Grouping and model building

[0056] 64 guinea pigs aged 2 weeks were selected and randomly divided into illumination groups (natural light group, LED light group, fluorescent light group, full-spectrum LED light group) and illumination diet group (natural light diet group, LED light diet group, fluorescent light diet group, full-spectrum LED light diet group), 8 in each group. The natural light group and the natural light diet group were kept near the south window with natural light entering. The other groups were set up in a room without other light sources, and the different illumination groups were shielded with blackout cloth. The artificial light group was controlled by a timer for switch, and the time was set at 06:00-18:00. The experimental observation time was 12 weeks.

[0057] (2) Experimental herbs

[0058] Daily diet therapy recipe: Noon yang-tonifying recipe: walnut powder 10g, chestnut powder 10g, donkey-hide gelatin 10g, black bean 10g, mung bean 10g; midnight yin-nourishing recipe: pig spinal cord 30g, tortoise shell powder 10g, wolfberry 20g, chrysanthemum 10g, white fungus 10g, lily 20g, lotus seeds 10g, corn 20g.

[0059] (3) Method of administration

[0060] The dosage of the daily clock diet therapy prescription was calculated based on the equivalent dose ratio converted from the body surface area of ​​an adult of 70 kg. The guinea pigs were weighed every 2 weeks, and the total amount of medicine required every 2 weeks was dried and ground into powder, and added to conventional feed to make special feeds of "Zi Shi Yang Yin Prescription" and "Wu Shi Bu Yang Prescription". All groups were fed twice a day, at 10:00 and 22:00 respectively. The light group was fed with conventional feed, and the diet therapy group was fed with the Wu Shi Bu Yang Prescription special feed in the morning and the Zi Shi Yang Yin Prescription special feed in the evening for 12 consecutive weeks.

[0061] (4) Measurement of guinea pig eye axis and refractive power

[0062] The axial length of each group of guinea pigs was measured by A-ultrasound before and every 2 weeks after the experiment. The probe was aimed at the pupil, perpendicular to the corneal plane to avoid compression. The waveform was stable and the peak was clear. The data of both eyes were recorded 5 times to obtain the average value. All measurements were performed by two fixed professionals. The refractive power of both eyes of the guinea pigs was measured by using a strip light ophthalmoscope before and every 4 weeks after the experiment. Tropicamide eye drops were dripped into both eyes before the examination, once every 5 minutes, for a total of 3 times. Retinoscopy was performed by an experienced optometrist in a dark room. The final refractive power was determined according to the equivalent spherical principle (half of the astigmatism was counted into the spherical lens). Each guinea pig was measured 3 times, and the average value was taken and the refractive power was recorded.

[0063] 2. Experimental results

[0064] SPSS 27.0 statistical analysis software was used for analysis, normal distribution and variance homogeneity test, and the data results were expressed as mean ± standard deviation. The independent sample T test was used for comparison between two groups, and one-way analysis of variance was used for comparison between multiple groups. P < 0.05 indicated statistically significant differences.

[0065] (1) Comparison of axial length

[0066] There was no significant difference in the axial length of guinea pigs in each group before the experiment (P>0.05). After the experiment, the axial length of guinea pigs in each group increased. The axial length of guinea pigs in the LED light and fluorescent light groups increased rapidly, which was greater than that in the natural light group in the fourth week of the experiment (P<0.01, P<0.05), and there were statistical differences in the sixth, eighth, tenth, and twelfth weeks. After the intervention of the dietary therapy, the axial length growth trend of the LED light dietary therapy group and the fluorescent light dietary therapy group slowed down, and the length was less than that of the LED light group and the fluorescent light group (P<0.01, P<0.05), and there were statistical differences in the fourth, sixth, eighth, tenth, and twelfth weeks. Within the 12 weeks of the experiment, there was no statistical difference in the axial length between the full-spectrum LED light group and the natural light group (P>0.05) (Table 1).

[0067] Table 1 Comparison of axial length of guinea pigs in each group (mm)

[0068]

[0069] Note: Compared with the natural light group, * P<0.05, ** P<0.01; compared with the LED light group, ## P<0.01; compared with the fluorescent light group, △ P<0.05, △△ P<0.01.

[0070] (2) Refractive power comparison

[0071] There was no statistical difference in the refraction of guinea pigs in each group before the experiment. After 4 weeks of the experiment, the hyperopia refraction of guinea pigs in each group decreased to varying degrees, and the LED group was the most obvious, and there was a difference between the natural light group (P < 0.05); after 8 weeks of the experiment, there was a difference between the fluorescent light group and the natural light group (P < 0.01), and the LED light group showed a trend of myopia; in the 12th week of the experiment, the LED light group and the fluorescent light group showed different degrees of myopia, and the difference between them and the natural light group was significant (all P < 0.01), and there was no statistical difference in the refraction of the full-spectrum LED light group and the natural light group at each week. After the intervention of the dietary therapy, the degree of myopia shift in the LED light dietary therapy group and the fluorescent light dietary therapy group was significantly reduced, and there was a difference between them and the LED light group and the fluorescent light group at the 4th and 8th weeks of the experiment, respectively (P < 0.01), and they were still in the hyperopia state at the 12th week of the experiment; there was no statistical difference in the refraction of the full-spectrum LED dietary therapy group compared with the full-spectrum LED light group at each week (Table 2).

[0072] Table 2 Comparison of diopter of guinea pigs in each group (D)

[0073] Group Week 0 Week 4 Week 8 Week 12 Natural light group 3.58±0.85 2.31±0.63 1.5±0.66 0.8±0.47 LED Light Set 3.55±0.48 <![CDATA[1.63±0.72 * ]]> <![CDATA[-0.42±0.76 ** ]]> <![CDATA[-1.64±0.65 ** ]]> Fluorescent lamp set 3.44±0.74 2.14±0.66 <![CDATA[0.2±0.87 ** ]]> <![CDATA[-0.63±0.79 ** ]]> Full spectrum LED light set 3.61±0.43 2.48±0.5 1.3±0.53 0.69±0.52 Natural light diet group 3.56±0.87 2.77±0.77 1.72±0.49 1.02±0.53 LED light diet therapy group 3.44±0.63 <![CDATA[2.23±0.37 ## ]]> <![CDATA[1.05±0.39 ## ]]> <![CDATA[0.45±0.41 ## ]]> Fluorescent light diet group 3.56±0.93 2.42±0.66 <![CDATA[1.36±0.66 △△ ]]> <![CDATA[0.5±0.58 △△ ]]> Full spectrum LED light diet therapy set 3.69±1.21 2.38±0.86 1.44±0.71 0.73±0.6

[0074] Note: Compared with the natural light group, * P<0.05, **P<0.01; compared with the LED light group, ## P<0.01; compared with the fluorescent light group, △ P<0.05, △△ P<0.01.

[0075] Example 5: Evaluation of retinal tissue morphology

[0076] 1. Experimental methods

[0077] The guinea pigs raised for 12 weeks in Example 4 were anesthetized by intraperitoneal overdose injection of 20% urethane solution (0.6 mL / 100 g) and then the eyeballs were removed. Three eyeballs were randomly selected from each group and fixed in 2.5% glutaraldehyde solution. The remaining eyeballs were dissected on ice, the anterior segment and vitreous tissue were removed, and the retina was peeled off under a microscope and stored in a -80°C refrigerator.

[0078] The guinea pig eyeballs were removed from the eyeball fixative, dehydrated with gradient ethanol, embedded in paraffin, and sliced ​​longitudinally along the optic nerve at a thickness of 3 μm. The sections were dewaxed to water, stained with HE, and then the excess staining solution was washed away. The sections were dehydrated with gradient alcohol, stained with eosin for 5 minutes, placed in anhydrous ethanol 3 times and xylene 2 times, each time for 5 minutes, until transparent, and sealed with neutral gum. The tissue morphology of the retinal structure was observed under a microscope and photographed.

[0079] 2. Experimental results

[0080] After 12 weeks of intervention, HE staining results showed ( Figure 1 ), the retinal layers of guinea pigs in the natural light group were arranged neatly, the ganglion cell layer was evenly distributed, and the inner and outer nuclear layer cells were closely arranged. The retinal thickness of guinea pigs in the LED light group and fluorescent light group became thinner, the ganglion cell nuclei were sparse, vacuolated obviously, the number of inner and outer nuclear layer cells decreased significantly, the cell distribution gap increased, and the outer nuclear layer cells were arranged in disorder. The full-spectrum LED light group showed thinning retinal thickness, but compared with the LED light and fluorescent light groups, the number of ganglion cells, inner and outer nuclear layer cells was relatively large, and the outer nuclear layer cells were arranged relatively neatly. After the dietary intervention, the retinal tissue of the artificial light diet therapy group was thicker than that of the same light source illumination group, the ganglion cell vacuolation phenomenon was improved, the number of ganglion cells, inner and outer nuclear layer cells increased significantly, and the outer nuclear layer cells were arranged relatively neatly.

[0081] Example 6: Evaluation of dopamine and retinoic acid content in the retina

[0082] 1. Experimental methods

[0083] The retinal tissue preserved in Example 5 was used, 100 mg of retinal tissue was accurately weighed for each group, and 2 ml of methanol: ethyl acetate: glacial acetic acid (1:3:0.01) was added for extraction, vortexed for 5 min, centrifuged at 10000 r / min for 10 min, the supernatant was taken, dried by N2, re-dissolved in 50% methanol-1% acetic acid water, centrifuged again for 10 min, and the supernatant was transferred to the sample bottle of the automatic sampler, and the injection volume was 20 μL, the mobile phase was methanol-water-glacial acetic acid (50:50:1), the flow rate was 1.0 mL / min, and the column temperature was 30° C. High performance liquid chromatography was used for simultaneous dual wavelength detection at 280 nm (dopamine) and 340 nm (retinoic acid), the image was analyzed, a standard curve was established, and the peak area was calculated.

[0084] 2. Experimental results

[0085] SPSS 27.0 statistical analysis software was used for analysis, and the data were expressed as mean ± standard deviation. The independent sample T test was used for comparison between two groups, and one-way analysis of variance was used for comparison between multiple groups. P < 0.05 indicated statistically significant differences.

[0086] After 12 weeks of the experiment, the retinoic acid level in the retinal tissue of the guinea pigs in the artificial light group was higher than that in the natural light group (P < 0.05), and the dopamine level was significantly lower than that in the natural light group (P < 0.01). After the intervention of the dietary therapy, the retinal dopamine level of each dietary therapy group was significantly increased, and the retinoic acid level was significantly decreased, which was statistically different from the same light source group (P < 0.05) (Table 3).

[0087] Table 3 Contents of retinoic acid and dopamine in the retinal tissue of guinea pigs in each group

[0088]

[0089]

[0090] Note: Compared with the natural light group, * P<0.05, ** P<0.01; compared with the LED light group, ## P<0.01; compared with the fluorescent light group, △ P<0.05, △△ P<0.01; compared with the full-spectrum LED light group, $$ P<0.01.

[0091] Example 7: Evaluation of relative expression levels of RAR-β, cRABP-I, and DRD2 proteins in the retina

[0092] 1. Experimental methods

[0093] The retinal tissue preserved in Example 5 was used to take retinal tissue from each group, add lysis solution and grind, lyse in ice water for 30 minutes, centrifuge at 12000r / min for 5 minutes, take supernatant, detect protein concentration using BCA kit, mix the extracted protein supernatant with protein loading buffer at a volume ratio of 4:1, boil in 100°C water bath for 10 minutes, cool to room temperature, perform electrophoresis, transfer, block, add primary antibody and incubate at 4°C overnight, wash the membrane, incubate with secondary antibody at 4°C for 2 hours, wash away excess secondary antibody, select chemiluminescence, develop and expose, and analyze gray value using Image J software.

[0094] 2. Experimental results

[0095] SPSS 27.0 statistical analysis software was used for analysis, and the data were expressed as mean ± standard deviation. The independent sample T test was used for comparison between two groups, and one-way analysis of variance was used for comparison between multiple groups. P < 0.05 indicated statistically significant differences.

[0096] Western blot test showed that after 12 weeks of the experiment, compared with the natural light group, the expression levels of RAR-β and cRABP-I proteins in the retinal tissue of guinea pigs in the artificial light group were significantly increased (P < 0.01), and the expression level of DRD2 protein was significantly decreased (P < 0.01). After the intervention of the dietary therapy, the expression levels of RAR-β and cRABP-I proteins in the retinal tissue of guinea pigs in each dietary therapy group were significantly decreased compared with the same light source group (all P < 0.01), and the expression level of DRD2 protein was significantly increased (P < 0.01) (Table 4, Figure 2 ).

[0097] Table 4 Expression results of RAR-β, CRABP-I and DRD2 proteins in retinal tissues of guinea pigs in each group

[0098] Group RARβ protein CRABP-1 protein DRD2 protein Natural light group 0.173±0.022 0.131±0.008 0.259±0.016 LED Light Set <![CDATA[0.863±0.025 ** ]]> <![CDATA[0.874±0.031 ** ]]> <![CDATA[0.037±0.003 ** ]]> Fluorescent lamp set <![CDATA[0.749±0.012 ** ]]> <![CDATA[0.697±0.042 ** ]]> <![CDATA[0.089±0.006 ** ]]> Full spectrum LED light set <![CDATA[0.662±0.023 ** ]]> <![CDATA[0.559±0.051 ** ]]> <![CDATA[0.205±0.032 ** ]]> Natural light diet group <![CDATA[0.112±0.008 ** ]]> <![CDATA[0.096±0.002 ** ]]> <![CDATA[0.717±0.046 ** ]]> LED light diet therapy group <![CDATA[0.739±0.016 ## ]]> <![CDATA[0.715±0.049 ## ]]> <![CDATA[0.084±0.01 ## ]]> Fluorescent light diet group <![CDATA[0.577±0.025 △△ ]]> <![CDATA[0.437±0.02 △△ ]]> <![CDATA[0.266±0.022 △△ ]]> Full spectrum LED light diet therapy set <![CDATA[0.397±0.023 $$ ]]> <![CDATA[0.279±0.01 $$ ]]> <![CDATA[0.469±0.026 $$ ]]>

[0099] Note: Compared with the natural light group, * P<0.05, ** P<0.01; compared with the LED light group, ## P<0.01; compared with the fluorescent light group, △ P<0.05, △△ P<0.01; compared with the full-spectrum LED light group, $$ P<0.01.

[0100] Example 8: Evaluation of the expression levels of RAR-β, cRABP-I, and DRD2 mRNA in the retina

[0101] 1. Experimental methods

[0102] The retinal tissues preserved in Example 5 were used to extract total RNA from the retinal tissues of guinea pigs in each group using the Trizol method. After adding the RT mixed reaction solution, reverse transcription reaction was performed to synthesize cDNA. PCR amplification was performed using the cDNA as a template, GAPDH was used as an internal reference gene, and the primer sequences were designed using Primer 5.0 software. The data were obtained using 2 -△△CT Method for analysis.

[0103] Primer sequences:

[0104]

[0105] 2. Experimental results

[0106] SPSS 27.0 statistical analysis software was used for analysis, and the data were expressed as mean ± standard deviation. The independent sample T test was used for comparison between two groups, and one-way analysis of variance was used for comparison between multiple groups. P < 0.05 indicated statistically significant differences.

[0107] RT-PCR test showed that after 12 weeks of the experiment, compared with the natural light group, the expression levels of RAR-β and cRABP-I mRNA genes in the retina of the artificial light group were significantly upregulated (P < 0.01), and the expression levels of DRD2 mRNA genes were significantly downregulated (P < 0.01). After the intervention of the dietary therapy, compared with the same light source group, the expression levels of RAR-β and cRABP-I mRNA genes in the retina of guinea pigs in each dietary therapy group were significantly downregulated (P < 0.01), and the expression levels of DRD2 mRNA genes were significantly upregulated (P < 0.01).

[0108] Table 5 Expression results of RAR-β, CRABP-I and DRD2 mRNA in the retina of guinea pigs in each group

[0109]

[0110]

[0111] Note: Compared with the natural light group, * P<0.05, ** P<0.01; compared with the LED light group, ## P<0.01; compared with the fluorescent light group, △ P<0.05, △△ P<0.01; compared with the full-spectrum LED light group, $$ P<0.01.

[0112] In summary, after the LED lamp and fluorescent lamp in the present invention caused myopia in guinea pigs, the dopamine content in the retina of the guinea pigs decreased significantly, and the mRNA and protein expression of DRD2 decreased. After the application of the daily clock diet, the dopamine content in the retinal tissue of the guinea pigs in each diet group increased, and the mRNA and protein expression of DRD2 increased, indicating that the daily clock diet can enhance the expression of DRD2 and increase the retinal dopamine content, thereby playing a refractive protection role. At the same time, the retinal ... The daily clock diet therapy can downregulate the mRNA and protein expression levels of RAR-β and CRABP-I, reduce the retinal retinal content of guinea pigs, and achieve better therapeutic effects in delaying axial growth and inhibiting the development of myopia.

[0113] The Sun-Clock Dietary Recipe selects foods with corresponding tastes and medicinal and edible properties according to the yin-yang properties of different hours of the day, adjusts the balance of qi and blood, yin and yang in the human body, is safer, more durable, and can achieve the advantages of overall conditioning. The above results confirm that the Sun-Clock Dietary Recipe can delay the growth of the guinea pig's axial length, inhibit myopic deviation, increase retinal thickness, increase dopamine concentration and D2 receptor expression in the guinea pig retina, downregulate the expression levels of RARβ and CRABP-I protein and mRNA, reduce retinoic acid concentration, inhibit the progression of myopia, and protect the retinal tissue structure. In general, the use of lighting fixtures with continuous spectrum and close to natural light can provide a more comfortable and healthy light environment, which helps to reduce the impact of inappropriate light sources on visual health. The Sun-Clock Dietary Recipe is made into a convenient and delicious snack form, which is not only convenient for teenagers to eat daily, but also has potential benefits for vision development and overall health. By improving the lighting environment and combining Chinese medicine diet therapy, it can be used as a comprehensive myopia prevention and control strategy, which is of great significance for improving the visual health of teenagers.

Claims

1. A Chinese medicine composition for preventing and treating myopia, characterized in that: The invention is composed of a noon yang-tonifying recipe and a midnight yin-nourishing recipe; measured by weight, the noon yang-tonifying recipe comprises 5-15 parts of walnuts, 5-15 parts of chestnuts, 3-10 parts of donkey-hide gelatin, 5-15 parts of black beans, and 5-15 parts of mung beans; the midnight yin-nourishing recipe comprises 20-50 parts of pig spinal cord, 10-20 parts of tortoise shells, 10-30 parts of wolfberries, 5-15 parts of chrysanthemums, 10-20 parts of lilies, 5-15 parts of white fungus, 5-15 parts of lotus seeds, and 10-30 parts of corn.

2. The Chinese medicine composition for preventing and treating myopia according to claim 1, characterized in that: The weight ratio of walnut, chestnut, donkey-hide gelatin, black bean and mung bean in the noon yang-tonifying recipe is 1:1:1:1:

1.

3. The Chinese medicine composition for preventing and treating myopia according to claim 2, characterized in that: By weight, the noon yang-tonifying recipe contains 10 parts of walnuts, 10 parts of chestnuts, 10 parts of donkey-hide gelatin, 10 parts of black beans, and 10 parts of mung beans.

4. The Chinese medicine composition for preventing and treating myopia according to claim 1, characterized in that: The weight ratio of pig spinal cord, tortoise shell, wolfberry, chrysanthemum, lily, white fungus, lotus seed and corn in the midnight nourishing yin recipe is 3:1:2:1:2:1:1:

2.

5. The Chinese medicine composition for preventing and treating myopia according to claim 4, characterized in that: By weight, the midnight yin nourishing recipe comprises 30 parts of pig spinal cord, 10 parts of tortoise shell, 20 parts of wolfberry, 10 parts of chrysanthemum, 20 parts of lily, 10 parts of white fungus, 10 parts of lotus seeds and 20 parts of corn.

6. The Chinese medicine composition for preventing and treating myopia according to claim 1, characterized in that: By weight, it includes 10 parts of donkey-hide gelatin, 10 parts of walnuts, 10 parts of chestnuts, 10 parts of black beans, 10 parts of mung beans, 20 parts of wolfberries, 30 parts of pig spinal cords, 10 parts of chrysanthemums, 10 parts of white fungus, 10 parts of tortoise shells, 20 parts of lilies, 10 parts of lotus seeds, and 20 parts of corn.

7. Use of the Chinese medicinal composition for preventing and treating myopia according to claim 1 in preparing medicines for preventing and treating myopia in children and adolescents.

8. The use according to claim 7, characterized in that: In the Chinese medicine composition, the noon yang-tonifying prescription is taken from 7 to 12 o'clock in the morning, and the midnight yin-nourishing prescription is taken from 17 to 21 o'clock in the evening.

9. The use according to claim 7, characterized in that: The medicine can increase the content of dopamine in the retina and reduce the content of retinoic acid in the retina.

10. The use according to claim 7, characterized in that: The drug can down-regulate the expression levels of RAR-β and cRABP-I proteins and mRNA in the retina, and up-regulate the expression levels of DRD2 proteins and mRNA in the retina.

Images