Device for identifying colquhoumia roots and adulterants of colquhoumia roots

Abstract

The invention belongs to the technical field of medicinal plant and traditional Chinese medicine identification, and discloses a device for identifying pyracantha formosana roots and adulterants thereof, the device comprises a liquid nitrogen ball mill, a DNA extraction kit and a PCR amplification instrument which are connected in sequence, the liquid nitrogen ball mill is connected with a variable speed motor, the device further comprises a sealing assembly, the sealing assembly comprises a first permanent magnet and a sealing ball, and the sealing ball is connected with the DNA extraction kit. The first permanent magnet and the sealing ball repel each other in magnetism, the first permanent magnet is arranged in the first through groove in a sliding mode, the sealing ball is arranged in the wedge-shaped through groove in a sliding mode and connected with a tension spring, and the free end of the tension spring is fixedly connected with the wedge-shaped through groove. The first through groove and the wedge-shaped through groove are each provided with a sliding groove, and the first permanent magnet and the sealing ball are in sliding connection with the sliding grooves correspondingly. The diameter of the sealing ball is not smaller than that of an opening of the wedge-shaped through groove. The liquid nitrogen supplementing amount can be adjusted according to the rotating speed change of the ball mill. The problem that an existing liquid nitrogen ball mill cannot adjust the liquid nitrogen supplementing amount according to the ball milling rotating speed is solved.

Description

Technical Field

[0001] The present invention belongs to the technical field of identification of medicinal plants and traditional Chinese medicines, and specifically relates to a device for identifying the root of torch flower and its mixed and counterfeit products. Background Art

[0002] Referring to the background technology with the existing public (announcement) number CN113862390A, Torch Flower Root and Tripterygium wilfordii both belong to the genus Tripterygium wilfordii of the Celastraceae family. Some suppliers on the market often use Torch Flower Root and other medicinal materials such as Tripterygium wilfordii interchangeably, and the medicinal parts are all roots. Due to the extremely similar appearance, the limited knowledge of the appraisers and other factors, it is difficult to distinguish Torch Flower Root from Tripterygium wilfordii, Northeast Tripterygium wilfordii, Celastrus orbiculatus and other medicinal materials, which seriously affects the market confusion and the accuracy and safety of medication.

[0003] To this end, the existing publication (announcement) number CN113862390A is a primer and method for identifying torch flower roots and their mixed products. The method for extracting genomic DNA from torch flower roots and their mixed products is as follows: 75% ethanol is used to scrub the surface of torch flower roots and their mixed products respectively, and they are crushed into fine pieces respectively, 60 mg of torch flower roots and their mixed products are taken respectively, and after adding liquid nitrogen respectively, they are ground with a fully automatic fast ball mill for 5 minutes, 30 times / s, to obtain samples of torch flower roots and their mixed products respectively; using an optimized plant DNA extraction kit, the samples of torch flower roots and their mixed products are respectively extracted according to the instructions of the kit to extract the total DNA of the samples, that is, the DNA templates of the torch flower roots and their mixed products are obtained. Therefore, to extract the genome of torch flower roots and their mixed products, it is necessary to use a liquid nitrogen ball mill to grind torch flower root slices and mixed products.

[0004] Referring to the existing publication (announcement) number CN109351444A, a refrigerated liquid nitrogen type ball mill includes a frame, a low-temperature resistant adapter and a grinding tank. The frame is provided with a support seat and a driving member for driving the grinding tank to rotate. The grinding tank is arranged on the support seat. One end of the low-temperature resistant adapter is detachably connected to the grinding tank, and the other end is connected to the refrigerated liquid nitrogen tank.

[0005] The ball mill connects the frozen liquid nitrogen tank with the grinding tank through a low-temperature adapter, so that liquid nitrogen is intermittently or continuously provided to the grinding tank so that the grinding tank is in a low-temperature environment, so that the grinding tank can crush or grind the material after freezing. Although the ball mill can intermittently or continuously provide liquid nitrogen to the grinding tank, when the ball mill grinds the material, due to the high-speed operation, the grinding balls in the grinding tank and the material and the tank wall constantly collide and rub, generating a large amount of heat energy, resulting in temperature rise; although the use of liquid nitrogen can maintain the initial low-temperature environment, the continuous friction and collision will still cause the temperature in the tank to gradually rise, especially when the speed is high, the rate of heat generation is faster. Excessive temperature can cause the hydrogen bonds of materials, such as DNA molecules in medicinal materials, to break, causing the double helix structure to unwind, thereby reducing the integrity of the DNA and affecting the accuracy of subsequent genome analysis and identification results. Therefore, liquid nitrogen supplementation and ball milling speed interact with the temperature in the ball mill. The higher the speed, the more frequent the collision and friction between the grinding balls in the ball mill and the material and the tank wall, resulting in temperature rise; and liquid nitrogen supplementation is to maintain the low-temperature environment in the mill. Therefore, it is necessary to adjust the amount of liquid nitrogen added according to the change in rotation speed to ensure that the temperature in the grinding tank remains within an appropriate range, thereby avoiding excessive temperature that may cause the hydrogen bonds in the DNA molecules in the medicinal materials to break, and ensuring the accuracy of subsequent genome analysis and identification results. Summary of the invention

[0006] The purpose of this scheme is to provide a device for distinguishing torch flower roots from their counterfeits, so as to solve the problem that the existing liquid nitrogen ball mill cannot adjust the liquid nitrogen replenishment amount according to the ball mill rotation speed.

[0007] In order to achieve the above-mentioned purpose, the present scheme provides a device for identifying torch flower roots and their counterfeits, comprising a liquid nitrogen ball mill, a DNA extraction kit and a PCR amplifier connected in sequence, the liquid nitrogen ball mill being connected to a variable speed motor, the feed port of the liquid nitrogen ball mill being provided with a first through slot, the first through slot being connected to the feed port, the bearing seat of the liquid nitrogen ball mill being provided with a wedge-shaped through slot, the wedge-shaped through slot being connected to the first through slot, the wedge-shaped through slot being connected to the liquid nitrogen tank through a pipeline; and also comprising a sealing assembly, the sealing assembly comprising a first permanent magnet and a sealing ball, the first permanent magnet and the sealing ball being magnetically repelling each other, the first permanent magnet being slidably disposed in the first through slot, the sealing ball being slidably disposed in the wedge-shaped through slot, and being connected to a tension spring, the free end of the tension spring being fixedly disposed in the pipeline connected to the wedge-shaped through slot.

[0008] The principle of this scheme is as follows: (1) Except for the ball mill grinding, the other identification steps are all existing technologies and will not be described in detail here. When the material is placed in the ball mill, the ball mill is driven to rotate by the variable speed motor. The first permanent magnet moves in the first slot under the centrifugal force generated by the rotation of the ball mill, so that the distance between the first permanent magnet and the sealing ball becomes smaller. Because the first permanent magnet and the sealing ball repel each other magnetically, the sealing ball is away from the opening of the wedge-shaped slot. The liquid nitrogen in the liquid nitrogen tank flows to the wedge-shaped slot through the pipeline, flows through the first slot and finally flows into the ball mill to mix with the medicinal materials. (2) When the speed of the ball mill rises to the critical speed, the first permanent magnet slides to the limit position of the first slot under the action of a large centrifugal force. At this time, the distance between the first permanent magnet and the sealing ball is the shortest, and the repulsion between the two is the largest, so that the sealing ball slides to the limit position in the wedge-shaped slot, thereby maximizing the opening volume of the wedge-shaped slot and maximizing the amount of liquid nitrogen flowing into the ball mill. (3) When the speed of the ball mill decreases from the critical speed, the first permanent magnet is subjected to a smaller centrifugal force, and when it rotates to the trajectory of the upper semicircle, it does not move to the limit position of the first through groove due to its own gravity. At this time, the distance between the first permanent magnet and the sealing ball increases, and the repulsive force between the two decreases, so that the sealing ball is driven by the tension spring to get closer to the opening of the wedge-shaped through groove, thereby reducing the opening volume of the wedge-shaped through groove and reducing the amount of liquid nitrogen flowing into the ball mill.

[0009] The effects of this solution are as follows: (1) Since liquid nitrogen is a liquid, the existing liquid nitrogen ball mill (see CN109351444A) is always at a constant speed after adding liquid nitrogen, which will cause the liquid to be subjected to a constant centrifugal force, causing the liquid nitrogen to adhere to the inner wall of the ball mill, affecting its mixing effect with the material. In this solution, since the speed is changing, the liquid nitrogen will produce disturbances in the ball mill under the action of the constantly changing centrifugal force. This disturbance will promote a more complete mixing of the liquid nitrogen and the material, thereby increasing the contact area and mixing effect between the liquid nitrogen and the material, ensuring the uniform distribution of the temperature in the grinding tank, and avoiding local overheating. (2) Because this solution requires a periodic change in the speed, that is, alternating between faster and slower, the temperature of the ball mill will be higher when the speed is faster and lower when the speed is slower. Therefore, it is necessary to adjust the amount of liquid nitrogen added according to the change in the speed so that the temperature of the medicinal materials is consistent during grinding. When the speed increases, the sealing ball slides to the limit position in the wedge-shaped groove, so that the opening of the wedge-shaped groove is maximized and the amount of liquid nitrogen flowing into the ball mill is maximized, avoiding the temperature increase caused by the speed and causing the DNA molecules in the medicinal materials to denature, thus ensuring the integrity of DNA extraction. When the speed is low, the sealing ball is closer to the opening of the wedge-shaped groove, so that the opening of the wedge-shaped groove is reduced, and the amount of liquid nitrogen flowing into the ball mill is reduced, so that the temperature of the medicinal materials ground at higher and lower speeds is consistent. (3) This scheme adjusts the replenishment of liquid nitrogen by changing the speed. Compared with the existing liquid nitrogen ball mill, it can maintain the temperature in the ball mill without stopping the machine, avoiding operation interruption and temperature fluctuation caused by stopping the machine to replenish liquid nitrogen.

[0010] Furthermore, both the first through groove and the wedge-shaped through groove are provided with sliding grooves, and the first permanent magnet and the sealing ball are slidably connected to the sliding grooves respectively.

[0011] The principle and effect of this solution are that the slide groove provides positioning and guiding functions for the sealing ball and the first permanent magnet to move in the first through groove and the wedge-shaped through groove under the action of centrifugal force.

[0012] Furthermore, the diameter of the sealing ball is not less than the diameter of the opening of the wedge-shaped through groove.

[0013] The principle and effect of this solution is that the sealing ball can completely block the opening of the wedge-shaped through groove under the action of centrifugal force, thereby sealing the wedge-shaped through groove.

[0014] Furthermore, the width of the first permanent magnet is smaller than the inner diameter of the first through slot.

[0015] The principle and effect of this solution is that a gap is formed between the first permanent magnet and the first through groove, and after the sealing ball is away from the wedge-shaped through groove, liquid nitrogen can flow into the feed port through the gap and into the ball mill jar.

[0016] Furthermore, the first through groove is an annular through groove, and the first permanent magnet is two symmetrically arranged semicircular permanent magnets, and the end surfaces of the two first permanent magnets are in contact with each other.

[0017] The principle and effect of this solution is that when the ball mill rotates, the first permanent magnet is always matched with the sealing ball.

[0018] Furthermore, the ball mill of the ball mill is provided with an inner cylinder and an outer cylinder, the inner cylinder is coaxially connected with the outer cylinder, a cavity is provided between the inner cylinder and the outer cylinder, and the inner cylinder is provided with a second through groove; it also includes a screening component, the screening component includes a baffle and a sieve plate, the baffle is fixedly connected to one end of the sieve plate, the baffle and the sieve plate are slidably arranged in the second through groove, the other end of the sieve plate is connected to a spring, the free end of the spring is fixedly connected to the inner cylinder, the sieve plate is connected to a second permanent magnet, an electromagnet is provided in the inner cylinder, and the electromagnet is used to attract the second permanent magnet when energized.

[0019] The principle and effect of this scheme are as follows: (1) Referring to the background technology of CN116351519A, after grinding the material, the ground powder needs to be poured out. In the process of pouring out the powder, the powder and the grinding beads are generally poured out together in the traditional way, and the powder and the grinding beads are screened by the screen later, which increases the operation steps and greatly reduces the efficiency of material grinding. (2) In this scheme, after the medicinal material is ground into fine powder, the electromagnet is energized to make its coil energized to attract the second permanent magnet, so that the sieve plate drives the baffle plate to move toward the electromagnet and compress the spring. Then the electromagnet is de-energized, and the sieve plate and the baffle plate are reset under the drive of the spring, and the sieve plate rotates back and forth in the second through groove, and the baffle plate is rotated away from the cavity and the sieve plate rotates back and forth in the cavity, so that the medicinal material powder is screened into the cavity, realizing the separation of the medicinal material powder and the grinding beads. (3) This solution provides a cavity between the inner cylinder and the outer cylinder and a sieve plate with a second permanent magnet, so that the medicinal material powder and the grinding beads can be separated directly in the ball mill after the grinding is completed, thereby simplifying the operation steps, avoiding the additional screening process required in the traditional method, and improving the grinding efficiency.

[0020] Furthermore, a discharge port is provided on the outer cylinder, the discharge port is communicated with the cavity, and a baffle plate is provided at the discharge port.

[0021] The principle and effect of this solution is that when discharging is required, the baffle plate is opened to discharge the powdered medicinal materials in the cavity out of the ball mill.

[0022] Furthermore, a guide groove is provided in the inner cylinder, and the baffle plate and the screen plate are both slidably connected to the guide groove.

[0023] The principle and effect of this solution are that the guide groove provides guidance for the movement of the baffle plate and the screen plate in the second through groove.

[0024] Furthermore, the baffle plate and the screen plate have the same curvature radius as the inner cylinder.

[0025] The principle and effect of this solution are to reduce the friction between the baffle plate and the sieve plate and the second through groove, and to increase the frequency of the baffle plate and the sieve plate rotating back and forth in the second through groove.

[0026] Furthermore, the screening assembly also includes a driving unit, which includes a touch switch and a return spring, the return spring is fixedly connected to the first through slot, the free end of the return spring is fixedly connected to the first permanent magnet, the touch switch is arranged in the first through slot, the first permanent magnet is used to resist the touch switch, and the touch switch is electrically connected to the electromagnet.

[0027] The principle and effect of this scheme are as follows: (1) When the ball mill rotates, the first permanent magnet is moved away from the touch switch by the centrifugal force and stretches the reset spring. At this time, the electromagnet is in a de-energized state. When the ball mill stops rotating, the first permanent magnet loses the centrifugal force and resets to the initial position driven by the reset spring, thereby contacting the touch switch, thereby giving the electromagnet an electric pulse signal, so that the electromagnet is powered on and off, thereby attracting the sieve plate and the baffle and screening the material under the drive of the spring after the power is cut off. (2) This scheme controls the power on and off of the electromagnet by changing the rotation speed because the existing technology will cause the material to stay in the ball mill for a longer time after the power is cut off, which is easy to cause the material to denature. However, this scheme controls the power on and off of the electromagnet by changing the rotation speed of the ball mill. When the ball mill stops rotating, the first permanent magnet resets and triggers the touch switch, so that the electromagnet is powered on and attracts the sieve plate and the baffle, thereby realizing automatic screening and discharging of the material. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 It is a partial structural schematic diagram of the liquid nitrogen ball mill of the present invention; Figure 2 The structure of the sealing assembly of the present invention is schematically shown in FIG. Figure 1 ; Figure 3 The structure of the sealing assembly of the present invention is schematically shown in FIG. Figure 2 ; Figure 4 The structure of the sealing assembly of the present invention is schematically shown in FIG. Figure 3 ; Figure 5 It is a schematic diagram of the structure of the screening assembly of the present invention.

[0029] The figure marks in the drawings of the specification include: liquid nitrogen ball mill 1, feed port 11, first through groove 111, bearing seat 12, wedge-shaped through groove 121, ball mill 13, inner cylinder 131, outer cylinder 132, cavity 133, sealing assembly 2, first permanent magnet 21, sealing ball 22, tension spring 23, screening assembly 3, baffle 31, sieve plate 32, spring 33, second permanent magnet 34, electromagnet 35, drive unit 36, touch switch 361, reset spring 362. DETAILED DESCRIPTION

[0030] The following will clearly and completely describe the concept and technical effects of the present invention in combination with the embodiments, so as to fully understand the purpose, features and effects of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, other embodiments obtained by those skilled in the art without creative work are all within the scope of protection of the present invention: Example: See also Figure 1 A device for identifying torch flower roots and their counterfeits includes a liquid nitrogen ball mill 1, a DNA extraction kit and a PCR amplifier connected in sequence. The liquid nitrogen ball mill 1 is connected to a variable speed motor. The above-mentioned equipment not shown in the figure is all prior art. The variable speed motor adopts a PID servo motor and is electrically connected to a PID controller.

[0031] See also Figure 2-Figure 4The feed port 11 of the liquid nitrogen ball mill 1 is provided with a first annular through groove 111, and the first through groove 111 is communicated with the feed port 11, so that the liquid nitrogen passing through the feed port 11 can flow into the first through groove 111 and flow into the ball mill 13. The top of the bearing seat 12 of the liquid nitrogen ball mill 1 is provided with a wedge-shaped through groove 121, and the wedge-shaped through groove 121 is communicated with the first through groove 111. The wedge-shaped through groove 121 is communicated with the liquid nitrogen tank (not shown in the figure) through a pipeline. The liquid nitrogen flows into the wedge-shaped through groove 121 from the pipeline connected to the liquid nitrogen tank, and then flows to the first through groove 111 and flows into the ball mill 13 to mix with the torch flower root slices and their impurities. The invention also includes a sealing component 2, which includes a first permanent magnet 21 and a sealing ball 22. The first permanent magnet 21 is two symmetrically arranged semicircular permanent magnets, and the end faces of the two first permanent magnets 21 fit each other, so that when the ball mill 13 rotates, the first permanent magnet 21 always cooperates with the sealing ball. The width of the first permanent magnet 21 is smaller than the inner diameter of the first through groove 111, so that there is a gap between the first permanent magnet 21 and the first through groove 111. After the sealing ball 22 is away from the wedge-shaped through groove 121, liquid nitrogen can flow into the feed port and into the ball mill 13 through the gap. The sealing ball 22 is provided with a sealing ball 22 that is aligned with the first permanent magnet 21. The third permanent magnet 21 repels the sealing ball 22, so that the sealing ball 22 and the first permanent magnet 21 repel each other magnetically. The diameter of the sealing ball 22 is not less than the diameter of the opening of the wedge-shaped through groove 121, so that the sealing ball 22 can completely block the opening of the wedge-shaped through groove 121 under the action of centrifugal force, thereby sealing the wedge-shaped through groove 121. The first through groove 111 is provided with a slide groove, the first permanent magnet 21 is slidably connected to the slide groove, the wedge-shaped through groove 121 is provided with a slide groove, the sealing ball 22 is slidably connected to the slide groove, the sealing ball 22 is connected with a tension spring 23, and the free end of the tension spring 23 is fixed in the pipe connected to the wedge-shaped through groove 121. It should be noted that the outer surfaces of the first permanent magnet 21, the sealing ball 22 and the tension spring 23 are all provided with an antifreeze coating to prevent liquid nitrogen from affecting their cooperation.

[0032] The specific working conditions are as follows: after the torch flower root slices and their counterfeits are placed in the ball mill 13, the ball mill 13 is driven to rotate by a variable speed motor, and the first permanent magnet 21 moves in the first through groove 111 under the centrifugal force generated by the rotation of the ball mill 13, so that the distance between the first permanent magnet 21 and the sealing ball 22 becomes smaller, and due to the magnetic repulsion between the first permanent magnet 21 and the sealing ball 22, the sealing ball 22 is kept away from the opening of the wedge-shaped through groove 121, and the liquid nitrogen in the liquid nitrogen tank flows through the pipeline to the wedge-shaped through groove 121, flows through the first through groove 111, and finally flows into the ball mill 13 to mix with the medicinal materials. When the ball mill speed rises to the critical speed (40-50r / min), the first permanent magnet 21 slides to the limit position of the first through groove 111 under the action of a large centrifugal force. At this time, the distance between the first permanent magnet 21 and the sealing ball 22 is the shortest, and the repulsive force between the two is the largest, so that the sealing ball 22 slides to the limit position in the wedge-shaped through groove 121, thereby maximizing the opening volume of the wedge-shaped through groove 121 and maximizing the amount of liquid nitrogen flowing into the ball mill jar 13 (see Figure 4 When the ball mill speed is reduced from the critical speed to (20-30r / min), the first permanent magnet 21 is subjected to a smaller centrifugal force, and when it rotates to the upper semicircle trajectory, it does not move to the limit position of the first through groove 111 due to its own gravity. At this time, the distance between the first permanent magnet 21 and the sealing ball 22 increases, and the repulsive force between the two decreases, so that the sealing ball 22 is driven by the tension spring 23 to get closer to the opening of the wedge-shaped through groove 121, thereby reducing the opening volume of the wedge-shaped through groove 121 and reducing the amount of liquid nitrogen flowing into the ball mill 13 (see Figure 3 ).

[0033] See also Figure 5 The ball mill 13 of the liquid nitrogen ball mill 1 is provided with an inner cylinder 131 and an outer cylinder 132, the inner cylinder 131 and the outer cylinder 132 are coaxially connected, a cavity 133 is provided between the inner cylinder 131 and the outer cylinder 132, a discharge port is provided on the outer cylinder 132, the discharge port is communicated with the cavity 133, a baffle plate is provided at the discharge port, when discharge is required, the powdered medicinal materials in the cavity are discharged to the outside of the ball mill by opening the baffle plate, and a second through groove is provided in the inner cylinder 131; the screening component 3 is also included, the screening component 3 includes a baffle plate 31 and a sieve plate 32, and the curvature radius of the baffle plate 31 and the sieve plate 32 is equal to the curvature radius of the inner cylinder 131, In order to reduce the friction between the baffle 31 and the sieve plate 32 and the second through groove and increase the frequency of the baffle 31 and the sieve plate 32 rotating back and forth in the second through groove, the baffle 31 and the sieve plate 32 are fixedly connected at one end, and the baffle 31 and the sieve plate 32 are slidably arranged in the second through groove, a guide groove is arranged in the inner cylinder 131, and the baffle 31 and the sieve plate 32 are both slidably connected to the guide groove, and the other end of the sieve plate 32 is connected to a spring 33, and the free end of the spring 33 is fixedly connected to the inner cylinder 131, and the sieve plate 32 is connected to the second permanent magnet 34, and an electromagnet 35 is arranged in the inner cylinder 131, and the electromagnet 35 is used to attract the second permanent magnet 34 when energized.

[0034] Specific working conditions: After the medicinal material is ground into fine powder, the electromagnet 35 is energized to make its coil energized to attract the second permanent magnet 34, so that the sieve plate 32 drives the baffle 31 to move toward the electromagnet 35 and compress the spring 33. Then the electromagnet 35 is de-energized, the sieve plate 32 and the baffle 31 are reset under the drive of the spring 33, and the sieve plate 32 rotates back and forth in the second through groove, and the baffle 31 rotates away from the cavity 133 and the sieve plate 32 rotates back and forth in the cavity 133, so that the medicinal material powder is sieved into the cavity 133, and the medicinal material powder is separated from the grinding beads, and finally the baffle plate is opened to discharge the material.

[0035] See also Figure 2-Figure 4 The screening assembly 3 further includes a driving unit 36, which includes a touch switch 361 and a reset spring 362. The reset spring 362 is provided with an antifreeze coating, the reset spring 362 is fixedly connected to the first through slot 111, the free end of the reset spring 362 is fixedly connected to the first permanent magnet 21, the touch switch 361 is arranged in the first through slot 111, and a flexible protective sleeve is arranged outside the touch switch 361. The first permanent magnet 21 is used to contact the touch switch 361, and the touch switch 361 is electrically connected to the electromagnet 35, and is used to give the electromagnet 35 an electric pulse after being contacted. When the ball mill 13 rotates, the first permanent magnet 21 is moved away from the touch switch 361 by the centrifugal force, and the reset spring 362 is stretched. At this time, the electromagnet 35 is in a power-off state. When the ball mill 13 stops rotating, the first permanent magnet 21 loses the centrifugal force and is reset to the initial position driven by the reset spring 362, thereby contacting the touch switch 361, thereby giving an electrical pulse signal to the electromagnet 35, causing the electromagnet 35 to be powered on and off, thereby attracting the screen plate 32 and the baffle 31 and screening the material driven by the spring 33 after power failure.

[0036] The above is only an embodiment of the present invention, and the common knowledge such as the known specific structure and characteristics in the scheme is not described in detail here. It should be pointed out that for those skilled in the art, several deformations and improvements can be made without departing from the structure of the present invention, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. A device for distinguishing torch flower roots from their counterfeits, comprising a liquid nitrogen ball mill (1), a DNA extraction kit and a PCR amplification instrument connected in sequence, characterized in that; The liquid nitrogen ball mill (1) is connected to a variable speed motor; a feed port (11) of the liquid nitrogen ball mill (1) is provided with a first through slot (111), the first through slot (111) being in communication with the feed port (11); a bearing seat (12) of the liquid nitrogen ball mill (1) is provided with a wedge-shaped through slot (121), the wedge-shaped through slot (121) being in communication with the first through slot (111), the wedge-shaped through slot (121) being in communication with the liquid nitrogen tank via a pipeline; and a sealing The sealing assembly (2) comprises a first permanent magnet (21) and a sealing ball (22), wherein the first permanent magnet (21) and the sealing ball (22) are magnetically repelled from each other, the first permanent magnet (21) is slidably disposed in a first through groove (111), the sealing ball (22) is slidably disposed in a wedge-shaped through groove (121), and is connected to a tension spring (23), wherein a free end of the tension spring (23) is fixedly disposed in a pipe connected to the wedge-shaped through groove (121).

2. A device for distinguishing torch flower roots from their counterfeits according to claim 1, characterized in that: The first through groove (111) and the wedge-shaped through groove (121) are both provided with sliding grooves, and the first permanent magnet (21) and the sealing ball (22) are respectively slidably connected to the sliding grooves.

3. A device for distinguishing torch flower roots from their counterfeits according to claim 1, characterized in that: The diameter of the sealing ball (22) is not less than the diameter of the opening of the wedge-shaped through groove (121).

4. The device for distinguishing torch flower roots from their counterfeits according to claim 1, characterized in that: The width of the first permanent magnet (21) is smaller than the inner diameter of the first through slot (111).

5. The device for distinguishing torch flower roots from their counterfeits according to claim 4, characterized in that: The first through groove (111) is an annular through groove, and the first permanent magnet (21) is two symmetrically arranged semicircular permanent magnets, and the end surfaces of the two first permanent magnets (21) are in contact with each other.

6. The device for distinguishing torch flower roots from their counterfeits according to claim 1, characterized in that: The ball mill (13) of the liquid nitrogen ball mill (1) is provided with an inner cylinder (131) and an outer cylinder (132), the inner cylinder (131) and the outer cylinder (132) being coaxially connected, a cavity (133) being provided between the inner cylinder (131) and the outer cylinder (132), and the inner cylinder (131) being provided with a second through slot; and further comprising a screening assembly (3), the screening assembly (3) comprising a baffle (31) and a screen plate (32), the baffle (31) and the screen plate (32) being connected to each other. One end of the plate (32) is fixedly connected, the baffle (31) and the sieve plate (32) are slidably arranged in the second through groove, the other end of the sieve plate (32) is connected to a spring (33), the free end of the spring (33) is fixedly connected to the inner cylinder (131), the sieve plate (32) is connected to a second permanent magnet (34), and an electromagnet (35) is arranged in the inner cylinder (131), and the electromagnet (35) is used to attract the second permanent magnet (34) when energized.

7. The device for distinguishing torch flower roots from their counterfeits according to claim 6, characterized in that: The outer cylinder (132) is provided with a discharge port, the discharge port is communicated with the cavity (133), and the discharge port is provided with a baffle plate.

8. The device for distinguishing torch flower roots from their counterfeits according to claim 6, characterized in that: A guide groove is provided in the inner cylinder (131), and the baffle plate (31) and the screen plate (32) are both slidably connected to the guide groove.

9. The device for distinguishing torch flower roots from their counterfeits according to claim 6, characterized in that: The baffle plate (31) and the screen plate (32) have the same curvature radius as the inner cylinder (131).

10. The device for distinguishing torch flower roots from their counterfeits according to claim 6, characterized in that: The screening assembly (3) further comprises a driving unit (36), the driving unit (36) comprising a touch switch (361) and a return spring (362), the return spring (362) being fixedly connected to the first through slot (111), the free end of the return spring (362) being fixedly connected to the first permanent magnet (21), the touch switch (361) being arranged in the first through slot (111), the first permanent magnet (21) being used to abut against the touch switch (361), and the touch switch (361) being electrically connected to the electromagnet (35).

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