Preparation method and application of functional fertilizer containing plant protein peptide

Abstract

The present invention relates to the technical field of biofertilizers, and in particular to a preparation method and application of a functional fertilizer containing plant protein peptides. The preparation steps of the functional fertilizer include: (1) raw material pretreatment: after shearing and grinding plant cake and seaweed, mixing with coffee grounds fermentation, adding a composite enzyme agent, and primary fermentation to obtain a pre-treated fermentation product; (2) preparation of a fermentation slurry containing plant protein peptides: evenly mixing a composite bacterial agent, modified bentonite, pre-treated fermentation product and deionized water, and secondary fermentation to obtain a fermentation slurry containing plant protein peptides; (3) preparation of a functional fertilizer: compounding the fermentation slurry containing plant protein peptides, wood vinegar and functional element liquid, adding an emulsifier and a stabilizer, and shearing and emulsifying to obtain a functional fertilizer containing plant protein peptides. The functional fertilizer prepared by the present invention has comprehensive nutrients, is easily absorbed by plant roots, has high biological activity, and can increase the yield and stress resistance of crops.

Description

Technical Field

[0001] The invention relates to the technical field of biological fertilizers, and more specifically to a preparation method and application of a functional fertilizer containing plant protein peptides. Background Art

[0002] It is of great significance to use modern science to find effective technologies to improve the soil microecological environment, increase soil fertility, promote the efficient and rapid growth of crops, increase crop yields, and achieve the goal of balanced development of ecological, economic and social benefits.

[0003] At present, the fertilizers on the market mainly include ammonium phosphate fertilizers, water-soluble fertilizers of large amounts of elements, fertilizers of medium amounts of elements, biological fertilizers, organic fertilizers, multi-dimensional field energy concentrated organic fertilizers, etc. However, most of them have a single nutrient. Long-term application will cause a decrease in soil organic matter, imbalance of various nutrients, soil acidification and compaction, and the microorganisms in the soil are susceptible to serious damage, resulting in poor soil water retention and air permeability, reduced fertility, and malnutrition of crops, affecting the growth and development of crops, reducing stress resistance, and reducing the yield and quality of crops. Therefore, there is an urgent need for a functional fertilizer that not only meets the requirements of comprehensive nutrients, easy absorption by plant roots, and high biological activity, but also is environmentally friendly, can improve soil structure, reduce soil compaction, increase the soil's ability to retain water and fertilizer, and enhance crop stress resistance. Summary of the invention

[0004] In order to solve the technical problems mentioned in the background technology, the present invention provides a method for preparing a functional fertilizer containing plant protein peptides.

[0005] One of the purposes of the present invention is to provide a method for preparing a functional fertilizer containing plant protein peptides, comprising the following steps:

[0006] (1) Raw material pretreatment: After the plant cake and seaweed are sheared and ground, they are mixed with the fermented coffee grounds, and a complex enzyme is added. The initial fermentation is carried out for 1-3 days to obtain the pre-treated fermented product;

[0007] (2) Preparation of plant protein peptide-containing fermentation slurry: the composite bacterial agent, modified bentonite, pre-treated fermentation product and deionized water are mixed evenly, and secondary fermentation is performed for 2-4 days to obtain plant protein peptide-containing fermentation slurry;

[0008] (3) Preparation of functional fertilizer: Compound the fermented slurry containing plant protein peptides, wood vinegar and functional element liquid, add an emulsifier, first shear at a low speed for 10 min-20 min, the shear rate is 500 rpm-1000 rpm, the temperature is 30°C-40°C, then adjust the shear rate to 5000 rpm-8000 rpm, the temperature is 42°C-50°C, and during this process, add a stabilizer, continue shearing and emulsification for 10 min-30 min, and cool naturally to obtain a functional fertilizer containing plant protein peptides.

[0009] Furthermore, in step (1), the plant cake is selected from one or more of soybean cake, peanut cake, sesame cake, rapeseed cake and sunflower seed cake.

[0010] Furthermore, in step (1), the seaweed is selected from one or more of the group consisting of ophiopogon, wakame, ulva, and kelp.

[0011] Furthermore, in step (1), the method for preparing the coffee grounds fermentation product is: coffee grounds, Trichoderma harzianum, waste molasses and deionized water are mixed in a mass ratio of 5-10:0.3-0.5:1-2:10-15, and fermented at a fermentation temperature of 40°C-45°C for 6-10 days to obtain the coffee grounds fermentation product.

[0012] Furthermore, in step (1), the mass ratio of plant cake, seaweed, fermented coffee grounds and complex enzyme is 150-200:50-70:100-150:5-10.

[0013] Furthermore, in step (1), the complex enzyme agent is one or more of protease, cellulase, amylase and xylanase.

[0014] Furthermore, in step (1), the complex enzyme agent is composed of protease, cellulase and amylase in a mass ratio of 5-8:1-2:1-3.

[0015] Furthermore, in step (2), the composite bacterial agent is composed of Bacillus subtilis, Lactobacillus plantarum and Azospirillum brasiliensis in an effective live bacterial count ratio of 10-12:5-6:1-2.

[0016] Furthermore, in step (2), the mass ratio of the composite bacterial agent, the modified bentonite, the pre-treated fermentation product and the deionized water is 2-3:150-180:300-400:800-1000.

[0017] Furthermore, in step (2), the secondary fermentation temperature is 30°C-35°C.

[0018] Furthermore, in step (2), the method for preparing the modified bentonite comprises the following steps:

[0019] Step S1, dispersing bentonite in a sodium hydroxide solution, adding polydimethyldiallylammonium chloride, ultrasonically reacting at 60° C.-80° C. for 1 h-4 h, filtering, washing until neutral, and drying at 80° C.-100° C. for 5 h-8 h to obtain activated bentonite;

[0020] Step S2, mixing the composite mineral liquid and activated bentonite, stirring evenly, adding the composite high molecular polymer, heating to 80°C-90°C, adding hydrochloric acid, adjusting the pH to 4-6, reacting for 2h-4h, washing to neutrality, and drying to obtain modified bentonite.

[0021] Furthermore, the composite mineral liquid in step S2 is composed of calcium ammonium nitrate, magnesium sulfate, dolomite and deionized water in a mass ratio of 1-3:1-2:0.1-0.5:120-150.

[0022] Furthermore, in step S2, the mass ratio of the composite mineral liquid, the activated bentonite and the composite high molecular polymer is 10-12: 3-4: 0.5-0.8.

[0023] Furthermore, in step S2, the composite high molecular polymer is composed of polylactic acid and polyvinyl alcohol in a mass ratio of 1-3:5-8.

[0024] Furthermore, in step (3), the mass ratio of the plant protein peptide fermentation slurry, wood vinegar, functional element liquid, emulsifier and stabilizer is 10-18:5-8:20-30:3-6:1-3.

[0025] Furthermore, in step (3), the emulsifier is one of lecithin, polysorbate, and lignin sulfonate.

[0026] Furthermore, in step (3), the stabilizer is one or more of sodium alginate, gum arabic and polysorbate.

[0027] Furthermore, in step (3), the functional element liquid includes a macroelement liquid, a medium element liquid and a trace element liquid.

[0028] Furthermore, the macro-element liquid is composed of nitrogen raw material, phosphorus raw material, potassium raw material and purified water in a mass ratio of 1-5:1-5:2-6:40-50.

[0029] Furthermore, the nitrogen raw material is one or more of urea, ammonium nitrate, calcium nitrate and ammonium sulfate.

[0030] Furthermore, the phosphorus raw material is one or more of phosphoric acid, diammonium phosphate and monoammonium phosphate.

[0031] Furthermore, the potassium raw material is potassium dihydrogen phosphate and / or dipotassium hydrogen phosphate.

[0032] Furthermore, the intermediate element liquid is composed of a calcium raw material, a magnesium raw material, a chelating agent and purified water in a mass ratio of 5-12:1-5:1-5:50-60.

[0033] Furthermore, the calcium raw material is calcium nitrate and / or slaked lime.

[0034] Furthermore, the magnesium raw material is magnesium nitrate and / or magnesium sulfate.

[0035] Furthermore, the trace element liquid is composed of zinc raw material, iron raw material, manganese raw material, molybdenum raw material, boron raw material, copper raw material, chelating agent and purified water in a mass ratio of 0.1-2:0.1-1:0.1-2:0.01-0.1:0.1-1:0.1-0.3:0.1-0.3:50-120.

[0036] Furthermore, the zinc raw material is zinc sulfate and / or zinc oxide.

[0037] Furthermore, the iron raw material is ferrous sulfate and / or ferric oxide.

[0038] Furthermore, the manganese raw material is manganese oxide and / or manganese sulfate.

[0039] Furthermore, the molybdenum raw material is ammonium molybdate and / or sodium molybdate.

[0040] Furthermore, the boron raw material is boric acid and / or borax.

[0041] Furthermore, the copper raw material is copper sulfate and / or copper oxide.

[0042] Furthermore, the chelating agent is ethylenediaminetetraacetic acid and / or diethylenetriaminepentaacetic acid.

[0043] The second purpose of the present invention is to provide a functional fertilizer containing plant protein peptides prepared by the above method for use in agricultural production.

[0044] In summary, the present invention has the following beneficial effects:

[0045] In the preparation process of the functional fertilizer containing plant protein peptides, the present invention successively performs primary fermentation and secondary fermentation. In the primary fermentation, the complex organic matter (such as cellulose, hemicellulose, protein, etc.) in the plant cake, seaweed and coffee ground fermentation has been partially decomposed, which improves the fermentability of the raw materials and can also provide a stable matrix (with good water retention and air permeability) for subsequent fermentation, which is conducive to long-term fermentation. The Trichoderma harzianum contained in the coffee ground fermentation of the present invention interacts with the composite bacterial agent during the primary fermentation and secondary fermentation process, which can improve the microbial ecology in the fermentation system, increase the diversity of microorganisms, and jointly deeply ferment the pre-treated fermentation to convert it into plant protein peptides. These plant protein peptides have better biological activity and nutritional functions, and can provide plants with more easily absorbed nitrogen sources and other nutrients. Modified bentonite can absorb some harmful substances produced during the fermentation process, such as heavy metal ions, excessive organic acids, etc., and improve the stability of fermentation products. At the same time, the adsorption performance of bentonite can also make microorganisms and nutrients in the fermentation process better adhere to its surface, reduce nutrient loss, and improve the quality of fermentation slurry. The composite bacterial agent and composite enzyme agent work together to accelerate the degradation of organic matter, generate more amino acids, peptides and other bioactive substances, and enhance the nutritional value of fertilizers. Brazilian nitrogen-fixing spirulina and Bacillus subtilis can not only produce a variety of plant growth regulators (such as indoleacetic acid, cytokinin and gibberellin, etc.), but also produce antibacterial substances to inhibit the growth of various pathogens in the soil, especially against soil-borne diseases (such as Fusarium, Pythium, etc.). Brazilian nitrogen-fixing spirulina and plant lactobacillus work synergistically to promote the circulation and transformation of nitrogen in the soil and increase the content of effective nitrogen in the soil. The three bacterial strain components of the present invention play a synergistic role, which can not only achieve efficient fermentation, but also promote the growth and yield of crops and enhance disease resistance in the subsequent agricultural production process. The high adsorption of nutrients and bacterial strains by modified bentonite is utilized to promote microbial activity, improve fermentation efficiency, improve soil structure, and prolong the slow-release effect of nutrients.

[0046] The present invention introduces polydimethyldiallylammonium chloride in the activation process of bentonite, which can increase the interlayer distance of bentonite, improve its adsorption performance, enhance the adhesion and colonization ability of microorganisms, and the activated bentonite has a higher cation exchange capacity. The composite mineral liquid is mixed with the activated bentonite, and through the joint action of polylactic acid and polyvinyl alcohol, a loose network structure is formed on the surface of bentonite, the specific surface area and porosity of the material are increased, and it is conducive to the attachment and growth of bacterial strains. A large number of hydroxyl groups in the polyvinyl alcohol molecule can form hydrogen bonds with the groups on the surface of bentonite. This hydrogen bonding action enables polyvinyl alcohol to be firmly attached to the surface of bentonite, increases the roughness and adsorption active sites of the surface of bentonite, and polyvinyl alcohol has good water solubility and viscosity. In a solution environment, it can increase the viscosity of the system, reduce the movement speed of the bacterial strain, and make the bacterial strain more easily adsorbed by bentonite. Moreover, polyvinyl alcohol can interact with polylactic acid to form a kind of interpenetrating network structure, which can not only enhance the mechanical strength of bentonite, but also form a more complex and stable adsorption layer on the surface of bentonite, and improve the adsorption capacity of different bacterial strains. The adsorption layer formed by polylactic acid and polyvinyl alcohol on the surface of bentonite can provide a relatively stable microenvironment for microorganisms, reduce the influence of external factors (such as temperature, pH change, etc.) on them, thereby maintaining the activity of microorganisms, and a large amount of mineral components adsorbed and fixed on the surface of modified bentonite can continuously provide nutrients for microorganisms and maintain the stability of fermentation matrix. After two fermentations, a large number of beneficial microorganisms are enriched in the fermentation slurry and adsorbed on the modified bentonite, thereby improving the microbial ecological environment of the soil and enhancing the stress resistance of plants.

[0047] The composite mineral liquid used in the preparation process of the modified bentonite of the present invention can not only regulate the permeability of the bacterial cell membrane, enhance the stress resistance of the bacterial strain, and promote the growth and reproduction of the bacterial strain, but also enhance the strength of the cell wall of crops, improve the disease resistance of plants, and reduce the occurrence of diseases. It also participates in the signal transduction and enzyme activation of plants, and can enhance the resistance of plants to adverse environmental conditions such as drought, salt stress, and low temperature, and enhances stress resistance. The quick-acting calcium ammonium nitrate and magnesium sulfate are combined with the long-acting dolomite to maintain the long-term fertility of the soil without affecting the growth of plants. The modified bentonite can also increase the air permeability and water retention capacity of the soil, and promote the growth and development of the root system. Especially in arid or semi-arid areas, the water retention performance of the modified bentonite can significantly improve the water retention capacity of the soil and prevent the soil from becoming compacted. DETAILED DESCRIPTION

[0048] The present invention is further described in detail below with reference to the embodiments.

[0049] The soybean cake used in the examples of the present invention and the comparative examples was purchased from Jize County Tiande Biological Feed Co., Ltd.; bentonite (water content: 30%) was purchased from Hongfa Bentonite Factory, Guoyuan Village, Angao Town, Wolong District, Nanyang City: Trichoderma harzianum Rifai (product name: Trichoderma harzianum Rifai, product number: B81740), Bacillus subtilis (product name: Bacillussubtilis, product number: B12039), Lactobacillus plantarum (product name: Lactobacillus plantarum, product number: BMZ133562), Aspergillus oryzae (product name: Aspergillus oryzae, product number: BMZ135482) and Azospirillum brasiliensis (product name: Azospirillum brasilense, product number: BMZ132766) was purchased from Ningbo Mingzhou Biotechnology Co., Ltd.; protease (product number: 24567678), cellulase (product number: 346578678) and amylase (product number: 1475) were purchased from Chengdu Wanxiang Hongrun Biotechnology Co., Ltd.; wood vinegar was purchased from Jinan Dasenlin Chemical Technology Co., Ltd.; dolomite (particle size: 1000-2000 mesh) was purchased from Lingshou Erping Mineral Products Processing Plant; polylactic acid was purchased from Shandong Lifan Chemical Co., Ltd.; polyvinyl alcohol (model: 2699) was purchased from Beijing Wantuming Technology Co., Ltd.; superoxide dismutase ELISA detection kit (product number: CB10021-Pt), catalase ELISA detection kit (product number: CB10048-Pt) and malondialdehyde ELISA detection kit (product number: CB10017-Pt) were all purchased from Shanghai Keaibo Biotechnology Co., Ltd.

[0050] Examples 1-3 and Comparative Examples 1-7 provide a method for preparing a functional fertilizer containing plant protein peptides.

[0051] Example 1

[0052] A method for preparing a functional fertilizer containing plant protein peptides, comprising the following preparation steps:

[0053] (1) Raw material pretreatment: soybean cake and seaweed are sheared and ground at a speed of 5000 rpm and a temperature of 40°C for 0.5 h, then passed through a 200-mesh sieve, mixed with coffee ground fermentation, and a composite enzyme agent is added. The initial fermentation is carried out for 1 day at a temperature of 25°C. The fermentation product is turned over every 3 hours to obtain a pre-treated fermentation product, wherein the mass ratio of soybean cake, seaweed, coffee ground fermentation product and composite enzyme agent is 30:10:20:1; the seaweed is ceratospermum officinale; the composite enzyme agent is composed of protease, cellulase and amylase in a mass ratio of 5:1:1; the preparation method of coffee ground fermentation product is as follows: coffee ground, Trichoderma harzianum, waste molasses and deionized water are mixed in a mass ratio of 5:0.3:1:10, fermented at a temperature of 40°C for 6 days, and the coffee ground fermentation product is obtained;

[0054] (2) Preparation of fermentation slurry containing plant protein peptides: The composite bacterial agent, modified bentonite, pre-treated fermentation product and deionized water in a mass ratio of 2:150:300:800 were mixed evenly, the fermentation tank was stirred at a speed of 150 rpm and a temperature of 30°C, and secondary fermentation was performed for 2 days to obtain a fermentation slurry containing plant protein peptides; wherein the composite bacterial agent was composed of Bacillus subtilis, Lactobacillus plantarum and Azospirillum brasiliensis in an effective live bacterial count ratio of 10:5:1;

[0055] Wherein, the preparation method of modified bentonite is:

[0056] Step S1, dispersing bentonite in a sodium hydroxide solution with a pH of 10, adding polydimethyldiallyl ammonium chloride, ultrasonically reacting at 60° C. for 1 h, with an ultrasonic power of 100 W and an ultrasonic frequency of 40 KHz, filtering, washing to neutrality, and drying at 80° C. for 5 h to obtain activated bentonite, wherein the mass ratio of bentonite, sodium hydroxide solution and polydimethyldiallyl ammonium chloride is 1:10:0.05;

[0057] Step S2, mixing the composite mineral liquid and activated bentonite, stirring evenly, adding the composite high molecular polymer, heating to 80°C, adding hydrochloric acid, adjusting the pH to 4, reacting for 2 hours, washing to neutrality, and drying to obtain modified bentonite. The mass ratio of the composite mineral liquid, activated bentonite, and composite high molecular polymer is 10:3:0.5; the composite mineral liquid is composed of calcium ammonium nitrate, magnesium sulfate, dolomite, and deionized water in a mass ratio of 1:1:0.1:120; and the composite high molecular polymer is composed of polylactic acid and polyvinyl alcohol in a mass ratio of 1:5.

[0058] (3) Preparation of functional fertilizer: Compounding the fermented slurry containing plant protein peptides, wood vinegar and functional element liquid, adding lecithin, firstly shearing at a low speed for 10 minutes, the shear rate is 500 rpm, the temperature is 30°C, then adjusting the shear rate to 5000 rpm, the temperature is 42°C, and in this process, adding sodium alginate, continuing shearing and emulsification for 10 minutes, and cooling naturally to obtain a functional fertilizer containing plant protein peptides, wherein the mass ratio of the fermented slurry containing plant protein peptides, wood vinegar, functional element liquid, lecithin and sodium alginate is 10:5:20:3:1; the functional element liquid includes a macroelement liquid, a medium element liquid and a trace element liquid;

[0059] The macronutrient solution is composed of urea, phosphoric acid, potassium dihydrogen phosphate and purified water in a mass ratio of 1:1:2:40;

[0060] The medium element solution is composed of calcium nitrate, magnesium sulfate, ethylenediaminetetraacetic acid and purified water in a mass ratio of 5:1:1:50;

[0061] The trace element liquid is composed of zinc sulfate, ferrous sulfate, manganese sulfate, ammonium molybdate, boric acid, copper sulfate, ethylenediaminetetraacetic acid and purified water in a mass ratio of 0.1:0.1:0.1:0.01:0.1:0.1, 0.1:50.

[0062] Example 2

[0063] A method for preparing a functional fertilizer containing plant protein peptides, comprising the following preparation steps:

[0064] (1) Raw material pretreatment: soybean cake and seaweed are sheared and ground at a speed of 5500 rpm and a temperature of 45°C for 0.8 h, then passed through a 250-mesh sieve, mixed with coffee ground fermentation, and a composite enzyme agent is added. The initial fermentation is carried out for 2 days at a temperature of 30°C. The fermentation product is turned over every 4 hours to obtain a pre-treated fermentation product, wherein the mass ratio of soybean cake, seaweed, coffee ground fermentation product and composite enzyme agent is 90:30:65:4; the seaweed is ceratospermum officinale; the composite enzyme agent is composed of protease, cellulase and amylase in a mass ratio of 6:1.5:2; the preparation method of coffee ground fermentation product is as follows: coffee ground, Trichoderma harzianum, waste molasses and deionized water are mixed in a mass ratio of 8:0.4:1.5:12, fermented at a temperature of 42°C for 8 days, and the coffee ground fermentation product is obtained;

[0065] (2) Preparation of fermentation slurry containing plant protein peptides: The composite bacterial agent, modified bentonite, pre-treated fermentation product and deionized water in a mass ratio of 2.5:160:350:900 were mixed evenly, the fermentation tank was stirred at a speed of 180 rpm and a temperature of 32°C, and secondary fermentation was performed for 3 days to obtain a fermentation slurry containing plant protein peptides; wherein the composite bacterial agent was composed of Bacillus subtilis, Lactobacillus plantarum and Azospirillum brasiliensis in an effective live bacterial count ratio of 11:5.5:1.5;

[0066] Wherein, the preparation method of modified bentonite is:

[0067] Step S1, dispersing bentonite in a sodium hydroxide solution with a pH of 11, adding polydimethyldiallyl ammonium chloride, ultrasonically reacting at 70° C. for 3 h, with an ultrasonic power of 110 W and an ultrasonic frequency of 50 KHz, filtering, washing until neutral, and drying at 90° C. for 6 h to obtain activated bentonite, wherein the mass ratio of bentonite, sodium hydroxide solution and polydimethyldiallyl ammonium chloride is 2:11:0.08;

[0068] Step S2, mixing the composite mineral liquid and activated bentonite, stirring evenly, adding the composite high molecular polymer, heating to 85°C, adding hydrochloric acid, adjusting the pH to 5, reacting for 3 hours, washing to neutrality, and drying to obtain modified bentonite. The mass ratio of the composite mineral liquid, activated bentonite, and composite high molecular polymer is 11:3.5:0.6; the composite mineral liquid is composed of calcium ammonium nitrate, magnesium sulfate, dolomite, and deionized water in a mass ratio of 2:1.5:0.3:135; and the composite high molecular polymer is composed of polylactic acid and polyvinyl alcohol in a mass ratio of 2:7.

[0069] (3) Preparation of functional fertilizer: Compounding the fermented slurry containing plant protein peptides, wood vinegar and functional element liquid, adding lecithin, firstly shearing at a low speed for 15 minutes, with a shear rate of 800 rpm and a temperature of 35°C, then adjusting the shear rate to 6000 rpm and a temperature of 450°C, and in the process, adding sodium alginate, continuing shearing and emulsification for 20 minutes, and cooling naturally to obtain a functional fertilizer containing plant protein peptides, wherein the mass ratio of the fermented slurry containing plant protein peptides, wood vinegar, functional element liquid, lecithin and sodium alginate is 14:6:25:4:2; the functional element liquid includes a macroelement liquid, a medium element liquid and a trace element liquid;

[0070] The macronutrient solution is composed of urea, phosphoric acid, potassium dihydrogen phosphate and purified water in a mass ratio of 3:3:4:45;

[0071] The medium element solution is composed of calcium nitrate, magnesium sulfate, ethylenediaminetetraacetic acid and purified water in a mass ratio of 7:3:3:55;

[0072] The trace element liquid is composed of zinc sulfate, ferrous sulfate, manganese sulfate, ammonium molybdate, boric acid, copper sulfate, ethylenediaminetetraacetic acid and purified water in mass ratios of 1:0.5:1:0.05:0.5:0.2, 0.2:70.

[0073] Example 3

[0074] A method for preparing a functional fertilizer containing plant protein peptides, comprising the following preparation steps:

[0075] (1) Raw material pretreatment: soybean cake and seaweed are sheared and ground at a speed of 6000 rpm and a temperature of 50°C for 1 hour, then passed through a 300-mesh sieve, mixed with coffee ground fermentation, and a composite enzyme agent is added. The initial fermentation is carried out for 3 days at a temperature of 35°C. The fermentation product is turned over every 5 hours to obtain a pre-treated fermentation product, wherein the mass ratio of soybean cake, seaweed, coffee ground fermentation product and composite enzyme agent is 20:7:15:1; the seaweed is ceratospermum officinale; the composite enzyme agent is composed of protease, cellulase and amylase in a mass ratio of 8:2:3; the preparation method of coffee ground fermentation product is as follows: coffee ground, Trichoderma harzianum, waste molasses and deionized water are mixed in a mass ratio of 10:0.5:2:15, fermented at a temperature of 45°C for 10 days, and the coffee ground fermentation product is obtained;

[0076] (2) Preparation of fermentation slurry containing plant protein peptides: The composite bacterial agent, modified bentonite, pre-treated fermentation product and deionized water in a mass ratio of 3:180:400:1000 were mixed evenly, the fermentation tank was stirred at a speed of 200 rpm, the temperature was 35°C, and the secondary fermentation was carried out for 4 days to obtain the fermentation slurry containing plant protein peptides; wherein the composite bacterial agent was composed of Bacillus subtilis, Lactobacillus plantarum and Azospirillum brasiliensis in an effective live bacterial count ratio of 6:3:1;

[0077] Wherein, the preparation method of modified bentonite is:

[0078] Step S1, dispersing bentonite in a sodium hydroxide solution with a pH of 13, adding polydimethyldiallyl ammonium chloride, ultrasonically reacting at 80° C. for 4 hours, with an ultrasonic power of 120 W and an ultrasonic frequency of 60 KHz, filtering, washing to neutrality, and drying at 100° C. for 8 hours to obtain activated bentonite, wherein the mass ratio of bentonite, sodium hydroxide solution and polydimethyldiallyl ammonium chloride is 3:12:0.1;

[0079] Step S2, mixing the composite mineral liquid and activated bentonite, stirring evenly, adding the composite high molecular polymer, heating to 90°C, adding hydrochloric acid, adjusting the pH to 6, reacting for 4 hours, washing to neutrality, and drying to obtain modified bentonite. The mass ratio of the composite mineral liquid, activated bentonite, and composite high molecular polymer is 12:4:0.8; the composite mineral liquid is composed of calcium ammonium nitrate, magnesium sulfate, dolomite, and deionized water in a mass ratio of 3:2:0.5:150; and the composite high molecular polymer is composed of polylactic acid and polyvinyl alcohol in a mass ratio of 3:8.

[0080] (3) Preparation of functional fertilizer: Compounding the fermented slurry containing plant protein peptides, wood vinegar and functional element liquid, adding lecithin, firstly shearing at a low speed for 20 minutes, with a shear rate of 1000 rpm and a temperature of 40°C, then adjusting the shear rate to 8000 rpm and a temperature of 50°C, and in the process, adding sodium alginate, continuing shearing and emulsification for 30 minutes, and cooling naturally to obtain a functional fertilizer containing plant protein peptides, wherein the mass ratio of the fermented slurry containing plant protein peptides, wood vinegar, functional element liquid, lecithin and sodium alginate is 18:8:30:6:3; the functional element liquid includes a macroelement liquid, a medium element liquid and a trace element liquid;

[0081] The macronutrient solution is composed of urea, phosphoric acid, potassium dihydrogen phosphate and purified water in a mass ratio of 5:5:6:50;

[0082] The medium element solution is composed of calcium nitrate, magnesium sulfate, ethylenediaminetetraacetic acid and purified water in a mass ratio of 12:5:5:60;

[0083] The trace element liquid is composed of zinc sulfate, ferrous sulfate, manganese sulfate, ammonium molybdate, boric acid, copper sulfate, ethylenediaminetetraacetic acid and purified water in a mass ratio of 2:1:2:0.1:1:0.3, 0.3:120.

[0084] Comparative Example 1

[0085] This comparative example is the same as Example 1, except that no composite enzyme agent is added during the raw material pretreatment process in step (1), and a composite bacterial agent and a composite enzyme agent are added during the preparation of the plant protein peptide fermentation slurry in step (2), as follows:

[0086] (1) Raw material pretreatment: soybean cake and seaweed are sheared and ground at a speed of 5000 rpm and a temperature of 40°C for 0.5 h, then passed through a 200-mesh sieve and mixed with coffee ground fermentation. The initial fermentation is carried out for 1 day at a temperature of 25°C. The fermentation is turned over every 3 hours to obtain a pre-treated fermentation product, wherein the mass ratio of plant cake, seaweed and coffee ground fermentation product is 3:1:2; the seaweed is Carrageenan; the preparation method of coffee ground fermentation product is as follows: coffee ground, Trichoderma harzianum, waste molasses and deionized water are mixed at a mass ratio of 5:0.3:1:10, the fermentation temperature is 40°C, and the fermentation is carried out for 6 days to obtain coffee ground fermentation product;

[0087] (2) Preparation of fermentation slurry containing plant protein peptides: The composite bacterial agent, composite enzyme agent, modified bentonite, pre-treated fermentation product and deionized water in a mass ratio of 2:5:150:300:800 were mixed evenly, the fermentation tank was stirred at a speed of 150 rpm, and secondary fermentation was carried out for 2 days to obtain the fermentation slurry containing plant protein peptides; wherein the composite bacterial agent was composed of Bacillus subtilis, Lactobacillus plantarum and Azospirillum brasiliensis in an effective live bacteria count ratio of 10:5:1; the composite enzyme agent was composed of protease, cellulase and amylase in a mass ratio of 5:1:1.

[0088] Comparative Example 2

[0089] This comparative example is the same as Example 1, except that a composite bacterial agent is added during the raw material pretreatment process in step (1), and a composite enzyme agent is added during the preparation of the plant protein peptide fermentation slurry in step (2), as follows:

[0090] (1) Raw material pretreatment: soybean cake and seaweed are sheared and ground at a speed of 5000 rpm and a temperature of 40°C for 0.5 h, then passed through a 200-mesh sieve, mixed with coffee ground fermentation, and a composite bacterial agent is added. The initial fermentation is carried out for 1 day at a temperature of 25°C. The fermentation product is turned over every 3 hours to obtain a pre-treated fermentation product, wherein the mass ratio of soybean cake, seaweed, coffee ground fermentation product and composite bacterial agent is 150:50:100:2; the seaweed is Carrageenan; the composite bacterial agent is composed of Bacillus subtilis, Lactobacillus plantarum and Azospirillum brasiliensis in an effective live bacteria count ratio of 10:5:1; the preparation method of coffee ground fermentation product is as follows: coffee ground, Trichoderma harzianum, waste molasses and deionized water are mixed in a mass ratio of 5:0.3:1:10, fermented at a temperature of 40°C for 6 days, and the coffee ground fermentation product is obtained;

[0091] (2) Preparation of fermentation slurry containing plant protein peptides: The composite enzyme agent, modified bentonite, pre-treated fermentation product and deionized water in a mass ratio of 5:150:350:800 were mixed evenly, the fermentation tank was stirred at a speed of 150 rpm, the temperature was 30°C, the humidity was 60%, and the secondary fermentation was carried out for 2 days to obtain the fermentation slurry containing plant protein peptides; wherein the composite enzyme agent was composed of protease, cellulase and amylase in a mass ratio of 5:1:1.

[0092] Comparative Example 3

[0093] This comparative example is the same as Example 1, except that the composition of the composite bacterial agent is different, as follows:

[0094] The composite bacterial agent is composed of Bacillus subtilis, Lactobacillus plantarum and Aspergillus oryzae in an effective live bacterial count ratio of 10:5:1;

[0095] Comparative Example 4

[0096] This comparative example is the same as Example 1, except that the preparation method of the modified bentonite is different, as follows:

[0097] The preparation method of modified bentonite is:

[0098] Step S1, dispersing bentonite in a sodium hydroxide solution with a pH of 10, subjecting the mixture to ultrasonic reaction at 60° C. for 1 h, with an ultrasonic power of 100 W and an ultrasonic frequency of 40 KHz, filtering, washing until neutral, and drying at 80° C. for 5 h to obtain activated bentonite, wherein the mass ratio of bentonite to the sodium hydroxide solution is 1:10;

[0099] Step S2, mixing the composite mineral liquid and activated bentonite, stirring evenly, adding the composite high molecular polymer, heating to 80°C, adding hydrochloric acid, adjusting the pH to 4, reacting for 2 hours, washing to neutrality, and drying to obtain modified bentonite. The mass ratio of the composite mineral liquid, activated bentonite, and composite high molecular polymer is 10:3:0.5; the composite mineral liquid is composed of calcium ammonium nitrate, magnesium sulfate, dolomite, and deionized water in a mass ratio of 1:1:0.1:120; and the composite high molecular polymer is composed of polylactic acid and polyvinyl alcohol in a mass ratio of 1:5.

[0100] Comparative Example 5

[0101] This comparative example is the same as Example 1, except that the preparation method of the modified bentonite is different, as follows:

[0102] The preparation method of modified bentonite is:

[0103] Step S1, dispersing bentonite in a sodium hydroxide solution with a pH of 10, adding polydimethyldiallyl ammonium chloride, ultrasonically reacting at 60° C. for 1 h, with an ultrasonic power of 100 W and an ultrasonic frequency of 40 KHz, filtering, washing to neutrality, and drying at 80° C. for 5 h to obtain activated bentonite, wherein the mass ratio of bentonite, sodium hydroxide solution and polydimethyldiallyl ammonium chloride is 1:10:0.05;

[0104] Step S2, mixing the composite mineral liquid and activated bentonite, stirring evenly, adding the composite high molecular polymer, heating to 80°C, adding hydrochloric acid, adjusting the pH to 4, reacting for 2 hours, washing to neutrality, and drying to obtain modified bentonite. The mass ratio of the composite mineral liquid, activated bentonite and composite high molecular polymer is 10:3:0.5; the composite mineral liquid is composed of calcium ammonium nitrate, magnesium sulfate and deionized water in a mass ratio of 1:1:120; and the composite high molecular polymer is composed of polylactic acid and polyvinyl alcohol in a mass ratio of 1:5.

[0105] Comparative Example 6

[0106] This comparative example is the same as Example 1, except that the preparation method of the modified bentonite is different, as follows:

[0107] The preparation method of modified bentonite is:

[0108] Step S1, dispersing bentonite in a sodium hydroxide solution with a pH of 10, adding polydimethyldiallyl ammonium chloride, ultrasonically reacting at 60° C. for 1 h, with an ultrasonic power of 100 W and an ultrasonic frequency of 40 KHz, filtering, washing to neutrality, and drying at 80° C. for 5 h to obtain activated bentonite, wherein the mass ratio of bentonite, sodium hydroxide solution and polydimethyldiallyl ammonium chloride is 1:10:0.05;

[0109] Step S2, mixing the composite mineral liquid and activated bentonite, stirring evenly, adding polylactic acid, heating to 80°C, adding hydrochloric acid, adjusting the pH to 4, reacting for 2 hours, washing to neutrality, and drying to obtain modified bentonite. The mass ratio of the composite mineral liquid, activated bentonite and polylactic acid is 10:3:0.5; the composite mineral liquid is composed of calcium ammonium nitrate, magnesium sulfate, dolomite and deionized water in a mass ratio of 1:1:0.1:120.

[0110] Comparative Example 7

[0111] This comparative example is the same as Example 1, except that the preparation method of the modified bentonite is different, as follows:

[0112] The preparation method of modified bentonite is:

[0113] Step S1, dispersing bentonite in a sodium hydroxide solution with a pH of 10, adding polydimethyldiallyl ammonium chloride, ultrasonically reacting at 60° C. for 1 h, with an ultrasonic power of 100 W and an ultrasonic frequency of 40 KHz, filtering, washing to neutrality, and drying at 80° C. for 5 h to obtain activated bentonite, wherein the mass ratio of bentonite, sodium hydroxide solution and polydimethyldiallyl ammonium chloride is 1:10:0.05;

[0114] Step S2, mixing the composite mineral liquid and activated bentonite, stirring evenly, adding polyvinyl alcohol, heating to 80°C, adding hydrochloric acid, adjusting the pH to 4, reacting for 2 hours, washing to neutrality, and drying to obtain modified bentonite. The mass ratio of the composite mineral liquid, activated bentonite and polyvinyl alcohol is 10:3:0.5; the composite mineral liquid is composed of calcium ammonium nitrate, magnesium sulfate, dolomite and deionized water in a mass ratio of 1:1:0.1:120.

[0115] Performance Testing

[0116] The comprehensive properties of the functional fertilizers containing plant protein peptides prepared in Examples 1-3 and Comparative Examples 1-7 of the present invention are as follows:

[0117] A Chinese cabbage yield increase test was conducted, 31 test plots were set up, the plot area was 5m×5m, 0.5m×5m protection rows were set between the plots, Chinese cabbage was planted, the variety was Jinlu 75, the sowing density was 3000 plants / mu, the field was managed uniformly, the fertilizer application rate was 30kg / mu each time, and the functional fertilizers prepared by Examples 1-3 of the present invention and Comparative Examples 1-7 were used for fertilization before sowing, 15 days after sowing, and 45 days after sowing, respectively. The blank group was not fertilized. After planting and harvesting, the index status of the Chinese cabbage plants was observed and evaluated:

[0118] The test results are shown in Table 1.

[0119] Table 1 Root weight per plant, yield and apparent fertilizer utilization rate of Chinese cabbage

[0120]

[0121]

[0122] A wheat field drought resistance experiment was conducted, 31 test plots were set up, the plot area was 5m×5m, 0.5m×5m protection rows were set between the plots, wheat was planted, the wheat variety was Bainong 418, the sowing density was 3×106 plants / mu, and the field was uniformly managed. Fertilization was performed before sowing, the fertilizer application rate was 50kg / mu, 40kg / mu was applied during the greening period, and 30kg / mu was applied again during the heading period, and then natural drought treatment was performed. After the wheat ears were fully formed, the superoxide dismutase (SOD) activity, catalase (CAT) activity, malondialdehyde (MDA) content and electrolyte exudation rate (REC) in the leaves of the wheat plants were measured using a detection kit. The five-point sampling method was used to measure the yield of wheat at the maturity stage of wheat, and the yield measurement area was 4m2. The functional fertilizer containing three different functional element solutions prepared in Examples 1-3 of the present invention and Comparative Examples 1-7 was used for fertilization, and no fertilization was performed on the blank group;

[0123] Electrolyte extravasation rate:

[0124] Sample preparation: Select wheat leaves from Examples 1-3, Comparative Examples 1-7 and the blank group and cut them into 1 cm² pieces. Prepare 6 replicate samples for each group of samples.

[0125] Initial conductivity determination: Determine the conductivity of deionized water (C0), then put the cut leaves into 10 mL of deionized water, use a conductivity meter to measure the initial conductivity (C1), and record the value;

[0126] Complete electrolyte release conductivity: Place the test tube containing the leaves in boiling water and boil for 15 minutes to completely destroy the cell membrane. After cooling to room temperature, use a conductivity meter to measure the conductivity (C2) after complete electrolyte release again and record the value.

[0127] REC=(C1-C0 / C2-C0)×100%

[0128] Yield increase rate = (yield of treatment group - yield of blank group) / yield of blank group × 100%

[0129] The statistical results are shown in Table 2.

[0130] Table 2 Detection of stress resistance of wheat

[0131]

[0132]

[0133] As can be seen from Table 1, the functional fertilizer containing three different functional element liquids prepared by the present invention has a single plant weight of 2.11kg-2.31kg / plant and an acreage yield of 6765.5kg-6963.7kg, indicating that the functional fertilizer prepared by the present invention can promote the growth of cabbage plants and increase yield.

[0134] As can be seen from Table 2, the functional fertilizer containing three different functional elements prepared by the present invention can significantly improve the stress resistance and antioxidant capacity of wheat. After using the functional fertilizer of the present invention, wheat shows excellent drought resistance, can significantly improve the superoxide dismutase (SOD) and catalase (CAT) activities in wheat leaves under drought conditions, and reduce the malondialdehyde (MDA) content in the root system and the electrolyte exudation rate of the leaves. These effects help wheat maintain normal physiological functions in a drought environment and reduce the damage caused by drought. More importantly, while improving drought resistance, the functional fertilizer can also significantly increase wheat yield, and the yield-increasing effect is very significant.

[0135] The embodiments of the present invention are merely explanations of the present invention and are not limitations of the present invention. After reading this specification, those skilled in the art may make modifications to the embodiments without any creative contribution as needed. However, such modifications are protected by the patent law as long as they are within the scope of the claims of the present invention.

Claims

1. A method for preparing a functional fertilizer containing plant protein peptides, characterized in that: The following steps are involved: (1) Raw material pretreatment: After the plant cake and seaweed are sheared and ground, they are mixed with the fermented coffee grounds, and a complex enzyme is added. The initial fermentation is carried out for 1-3 days to obtain the pre-treated fermented product; (2) Preparation of plant protein peptide-containing fermentation slurry: the composite bacterial agent, modified bentonite, pre-treated fermentation product and deionized water are mixed evenly, and secondary fermentation is performed for 2-4 days to obtain plant protein peptide-containing fermentation slurry; (3) Preparation of functional fertilizer: Compound the fermented slurry containing plant protein peptides, wood vinegar and functional element liquid, add an emulsifier, firstly perform low-speed shearing for 10-20 minutes, the shear rate is 500-1000 rpm, the temperature is 30°C-40°C, then adjust the shear rate to 5000-8000 rpm, the temperature is 42°C-50°C, and in this process, add a stabilizer, continue shearing and emulsification for 10-30 minutes, and cool naturally to obtain a functional fertilizer containing plant protein peptides; The complex enzyme agent is composed of protease, cellulase and amylase in a mass ratio of 5-8:1-2:1-3; The composite bacterial agent is composed of Bacillus subtilis, Lactobacillus plantarum and Azospirillum brasiliensis in an effective live bacterial count ratio of 10-12:5-6:1-2; the mass ratio of the composite bacterial agent, modified bentonite, pre-treatment fermentation product and deionized water is 2-3:150-180:300-400:800-1000; the secondary fermentation temperature is 30°C-35°C; The functional element liquid includes a large amount of element liquid, a medium amount of element liquid and a trace amount of element liquid; The preparation method of the modified bentonite comprises the following steps: Step S1, dispersing bentonite in a sodium hydroxide solution, adding polydimethyldiallylammonium chloride, ultrasonically reacting at 60° C.-80° C. for 1 h-4 h, filtering, washing until neutral, and drying at 80° C.-100° C. for 5 h-8 h to obtain activated bentonite; Step S2, mixing the composite mineral liquid and activated bentonite, stirring evenly, adding the composite high molecular polymer, heating to 80°C-90°C, adding hydrochloric acid, adjusting the pH to 4-6, reacting for 2h-4h, washing to neutrality, and drying to obtain modified bentonite; In step S2, the composite mineral liquid is composed of calcium ammonium nitrate, magnesium sulfate, dolomite and deionized water in a mass ratio of 1-3:1-2:0.1-0.5:120-150; the mass ratio of the composite mineral liquid, activated bentonite and composite high molecular polymer is 10-12:3-4:0.5-0.8; In the step S2, the composite high molecular polymer is composed of polylactic acid and polyvinyl alcohol in a mass ratio of 1-3:5-8.

2. The method for preparing the functional fertilizer containing plant protein peptide according to claim 1, characterized in that: In step (1), the plant cake is selected from one or more of soybean cake, peanut cake, sesame cake, rapeseed cake and sunflower seed cake; the mass ratio of the plant cake, seaweed, coffee ground fermentation product and complex enzyme is 150-200:50-70:100-150:5-10.

3. The method for preparing the functional fertilizer containing plant protein peptide according to claim 1, characterized in that: In step (1), the method for preparing the coffee grounds fermentation product is: coffee grounds, Trichoderma harzianum, waste molasses and deionized water are mixed in a mass ratio of 5-10:0.3-0.5:1-2:10-15, and fermented at a temperature of 40°C-45°C for 6-10 days to obtain the coffee grounds fermentation product.

4. The method for preparing the functional fertilizer containing plant protein peptide according to claim 1, characterized in that: In step (3), the mass ratio of the plant protein peptide fermentation slurry, wood vinegar, functional element liquid, emulsifier and stabilizer is 10-18:5-8:20-30:3-6:1-3.

5. Use of a functional fertilizer containing plant protein peptides prepared by the preparation method according to any one of claims 1 to 4 in agricultural production.

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