Biological deodorant for garbage station and preparation method of biological deodorant

Abstract

The invention discloses a biological deodorant for a garbage station and a preparation method of the biological deodorant, and belongs to the technical field of biological deodorization, the biological deodorant is prepared by mixing carefully screened saccharomyces cerevisiae, bacillus amyloliquefaciens, enterococcus faecalis and lactobacillus plantarum to form a compound microbial community, and culturing to obtain a compound microbial inoculum stock solution. The microbial carrier is prepared by combining natural materials such as kaolin, zeolite and diatomite with chemical synthetic materials such as polyether polyol and isocyanate, and carrying out high-temperature roasting and alkali liquor treatment to form the carrier with high adsorbability and stability. And finally, immersing the sterilized microbial carrier into the complex microbial inoculant stock solution to prepare the efficient and stable biological deodorant. Compared with the prior art, the biological deodorant prepared by the method shows excellent deodorization effect and long-term stability in a simulated garbage station environment, and a new solution is provided for stink control of the garbage station and other environments.

Description

Technical Field

[0001] The invention relates to the technical field of biological deodorization, and in particular to a biological deodorant for a garbage station and a preparation method thereof. Background Art

[0002] With the rapid development of industrialization and urbanization, the amount of urban domestic waste is increasing. As a key node for urban waste collection and transportation, garbage stations have particularly prominent environmental problems. Among them, the odorous gases produced by garbage stations not only affect the quality of life of surrounding residents, but also pose a potential threat to human health. Therefore, the development of efficient and environmentally friendly biological deodorants for garbage stations has important practical significance.

[0003] Traditional deodorization methods mainly rely on physical adsorption or chemical masking, such as activated carbon adsorption, ozone oxidation and other technologies, but these methods often have problems such as short-term deodorization effect and possible secondary pollution. As an emerging environmental protection technology, biological deodorization technology uses the metabolic activities of microorganisms to convert malodorous substances into harmless substances. It has the advantages of high treatment efficiency, no secondary pollution, and low operating costs, and has become a hot spot for research and application.

[0004] In the field of biological deodorization technology, the selection of microbial carriers is crucial. An ideal microbial carrier should have good biocompatibility, high specific surface area, suitable pore structure and mechanical strength to ensure efficient fixation and long-lasting activity of microorganisms. However, existing microbial carriers mostly use single materials, such as organic carriers such as polyacrylamide and polyvinyl alcohol, or inorganic carriers such as diatomaceous earth and porous ceramics. These carriers have certain limitations in terms of chemical stability, mechanical strength or biocompatibility.

[0005] In addition, the preparation of microbial agents is also a key factor affecting the biological deodorization effect. An ideal microbial agent should have strong ability to degrade odorous substances, good environmental adaptability and high activity stability. At present, although a variety of microbial agents have been developed for odor control, these agents often face problems such as rapid activity decline and poor stability in practical applications, which limits their long-term application in harsh environments such as garbage stations.

[0006] The biological deodorant of the present invention adopts a combination of natural mineral materials and chemical synthetic materials, which not only improves the immobilization effect of microorganisms and the mechanical properties of the carrier, but also enhances the chemical stability and biocompatibility of the carrier through a special post-treatment process. At the same time, the present invention improves the degradation ability and environmental adaptability of the bacterial agent by screening and optimizing the composite microbial community, providing a new solution for odor control in environments such as garbage stations.

[0007] Chinese invention patent CN110201204A discloses a biological deodorant carrier and a preparation method thereof, a biological deodorant and a preparation method thereof, and belongs to the field of biological deodorization technology. The biological deodorant carrier provided by the invention is prepared from the following raw materials in parts by weight: 9.5-10.0 parts of clay, 4.0-4.5 parts of attapulgite powder, 2.5-3.0 parts of calcium carbonate, and 1.8-2.5 parts of silicon dioxide. The biological deodorant carrier provided by the invention has excellent chemical stability, and its loss rate in strong acid, strong alkali, strong oxidant and alcohol solution is less than 6%. At the same time, it has a certain deodorization function, and when used in combination with a biological deodorizing bacterial agent, it has a more excellent deodorization performance. However, its deodorization effect and long-term stability still need to be improved. Summary of the invention

[0008] In view of the limitations of the prior art, the present invention aims to provide a biological deodorant for garbage stations and a preparation method thereof, which achieves efficient and long-lasting deodorization effect through innovative microbial carrier design and optimized preparation of microbial agents, and has good environmental adaptability and stability.

[0009] In order to achieve the above-mentioned object of the invention, the present invention adopts the following technical solutions: A method for preparing a biological deodorant for a garbage station is as follows, in parts by weight: Step 1, Saccharomyces cerevisiae, Bacillus amyloliquefaciens, Enterococcus faecalis, and Lactobacillus plantarum are mixed to form a composite microbial community; the composite microbial community is introduced into a tryptic soy bean liquid culture medium TSB for cultivation to promote its growth and reproduction, and after the cultivation process is completed, a composite bacterial agent stock solution is obtained; Step 2: sterilize the microbial carrier by dry heat, immerse the sterilized microbial carrier into the composite bacterial agent stock solution obtained in step 1, take out and drain in the air until there are no water droplets on the surface, and obtain a biological deodorant for garbage stations.

[0010] The brewer's yeast, Bacillus amyloliquefaciens, Enterococcus faecalis and Lactobacillus plantarum are mixed in a mass ratio of 2-4:0.5-2:0.5-2:1-3.

[0011] The composite microbial community is introduced into tryptic soy bean liquid culture medium TSB and cultured in a constant temperature box at 30-35° C. for 48-96 hours.

[0012] The microbial carrier is dry-heat sterilized at 100-150° C. for 1-5 hours.

[0013] The mass ratio of the microbial carrier to the composite bacterial agent stock solution obtained in step 1 is 0.5-2:2-4.

[0014] The microbial carrier is immersed in the composite bacterial agent stock solution obtained in step 1 for 5 to 24 hours.

[0015] The preparation method of the microbial carrier is as follows, in parts by weight: S1, weighing 0.5-1 parts of kaolin, 0.2-0.4 parts of zeolite and 0.05-0.2 parts of diatomaceous earth, mixing these raw materials with 2-4 parts of water to form a uniform slurry, making the slurry into wet spherical particles with a particle size of 0.2-0.5 cm, then placing these wet spherical particles in an environment of 15-25° C. for 5-24 hours to promote uniform distribution of moisture, and the aged particles enter a high-temperature calcination stage for calcination to form a pretreated carrier; S2, weighing 60-80 parts of polyether polyol, 10-20 parts of poly(1,4-butylene adipate), 5-10 parts of water, 1-3 parts of polydimethylsiloxane, 0.05-0.2 parts of stannous octoate, 0.4-0.6 parts of 2-hydroxyethyltriethylenediamine, and 30-50 parts of 1,2,3-trivinylbenzene, and fully mixing these raw materials in a reactor, maintaining the temperature in the reactor at 35-50° C. to promote interaction between the raw materials until a uniform mixture is formed, adding the pretreated carrier prepared in step S1, and continuing to stir to ensure that it is evenly dispersed in the mixture to form a post-treated product; S3, weighing 100-150 parts of 4,4'-benzylidene di(6-methyl-m-phenylene) tetraisocyanate and 0.05-0.2 parts of benzoyl peroxide, and mixing the two raw materials in a reaction kettle to ensure that the initiator is evenly dispersed to form a treating agent; S4. The post-treated product obtained in step S2 and the treatment agent prepared in step S3 are fully mixed in a reactor to ensure that the two are completely mixed. The mixed material is poured into a mold and subjected to a foaming reaction at room temperature for 10 to 20 hours. After the foaming is completed, the material is soaked in alkaline solution and then washed with water for 1 to 3 times to obtain a microbial carrier.

[0016] In the high temperature calcination stage, the calcination temperature is controlled at 700-900° C. and the duration is 2-4 hours.

[0017] The alkali solution soaking method is to immerse the material in the alkali solution, the mass ratio of the material to the alkali solution is 0.5-2:8-12, and the soaking treatment is 12-48 hours. The composition of the alkali solution is: 8-12 mol / L sodium hydroxide aqueous solution and ethylene glycol are prepared into a mixed solution in a volume ratio of 3-5:2-4.

[0018] In the present invention, the functions of each substance are as follows: Saccharomyces cerevisiae, Bacillus amyloliquefaciens, Enterococcus faecalis, Lactobacillus plantarum: These microorganisms are mixed in specific mass ratios to form a complex microbial community, which grow and multiply in tryptic soy broth TSB and produce various enzymes and metabolites that can decompose odor-producing compounds.

[0019] TSB provides nutrients and environment for the growth and reproduction of complex microbial communities.

[0020] Natural mineral materials such as kaolin, zeolite and diatomaceous earth are mixed to form the basic structure of the carrier, which is then calcined at high temperature to form a pretreated carrier with good adsorption and structural stability, providing attachment points for microorganisms.

[0021] Chemical synthetic materials such as polyether polyols, poly(1,4-butylene adipate) and polydimethylsiloxane are mixed with the pre-treated carrier in a reactor to form a post-treated product, which provides a protective and nutritional environment for the microorganisms and enhances the mechanical properties of the carrier.

[0022] The polyether polyol is a polymer with an ether bond (-ROR-) in the main chain and two or more hydroxyl groups (-OH) in the terminal or side group. It is usually prepared by ring-opening polymerization of a low molecular weight polyol, a polyamine or a compound containing active hydrogen as an initiator with an oxidized olefin (such as propylene oxide, ethylene oxide) under the action of a catalyst. The role of the polyether polyol in the present invention is to serve as one of the main raw materials for the formation of polyurethane foam, react with isocyanate to generate foam with a high specific surface area and excellent pore structure, provide a protective growth environment for microorganisms, enhance the mechanical strength and chemical stability of the microbial carrier, and thus improve the deodorization efficiency and long-term stability of the biological deodorant.

[0023] Stannous octoate and 2-hydroxyethyltriethylenediamine are used as catalysts to promote the chemical reaction between polyether polyol and isocyanate to form a stable polyurethane foam structure.

[0024] 1,2,3-Trivinylbenzene, as an active monomer, participates in the formation of a cross-linked network of polyurethane foam, thereby enhancing the stability and mechanical strength of the foam.

[0025] 4,4'-benzylidene di(6-methyl-m-phenylene) tetraisocyanate is used as an isocyanate raw material to react with polyether polyol and other components to form polyurethane foam. Its multiple isocyanate groups help to form a highly cross-linked network structure.

[0026] Benzoyl peroxide acts as a free radical initiator, triggering the polymerization reaction and promoting the formation of foam.

[0027] In the alkali solution soaking treatment, sodium hydroxide is used to further solidify the polyurethane foam, and ethylene glycol is used as a solvent to help adjust the viscosity and reactivity of the alkali solution. This step helps to improve the surface properties and bioaffinity of the carrier.

[0028] The final microbial carrier combines the advantages of natural mineral materials and chemical synthetic materials, providing a suitable growth environment for microorganisms while ensuring the mechanical strength and chemical stability of the carrier, enabling it to play an effective role in environments such as garbage stations.

[0029] Compared with the prior art, it has the following beneficial effects: 1) The present invention achieves a more efficient degradation capability for specific odor substances in the garbage station environment by screening and combining specific microbial communities, such as Saccharomyces cerevisiae, Bacillus amyloliquefaciens, Enterococcus faecalis and Lactobacillus plantarum. The synergistic effect of these microorganisms increases the degradation rate of odorous gases such as ammonia and hydrogen sulfide, thereby significantly improving the deodorization efficiency.

[0030] 2) By fixing the microorganisms on specially treated carriers, such as kaolin, zeolite and diatomaceous earth that have been calcined at high temperature and treated with alkali, and specific polyurethane foam, the biological deodorant of the present invention shows better long-term stability under simulated accelerated aging conditions. This stability ensures the long-lasting effect of the deodorant during storage and use, reduces the need for frequent replacement, and reduces maintenance costs.

[0031] 3) The present invention adopts a method for preparing a microbial carrier that combines natural mineral materials and chemical synthetic materials. This method not only improves the attachment and protection of microorganisms, but also optimizes the physical and chemical properties of the microbial carrier by precisely controlling the foaming and post-treatment processes. This optimized preparation process improves the production efficiency and quality control of the deodorant, making the product more in line with the needs of practical applications. DETAILED DESCRIPTION

[0032] Main sources of substances: Brewer's yeast, a commercially available product, comes from the China General Microbiological Culture Collection Center, with the Latin name Saccharomyces cerevisiae and the number CGMCC2.3973.

[0033] Bacillus amyloliquefaciens is a commercially available product from the China General Microbiological Culture Collection Center, with the scientific name Bacillus amyloliquefaciens and the number CGMCC1.857.

[0034] Enterococcus faecalis, a commercially available product, comes from the China General Microbiological Culture Collection Center, with the scientific name Enterococcus faecalis and the number CGMCC1.15424.

[0035] Lactobacillus plantarum, a commercially available product, comes from the China Agricultural Microbiological Culture Collection Center, with the scientific name Lactobacillus plantarum and the strain number ACCC 11118.

[0036] Tryptic soy casein broth TSB, catalog number: HB4114-19, Qingdao Hi-Tech Industrial Park Haibo Biotechnology Co., Ltd.

[0037] Kaolin, particle size: 800 mesh.

[0038] Zeolite, particle size: 200 mesh, silica content: 70-73 (%).

[0039] Diatomaceous earth, particle size: 100-200 mesh, SiO2 content ≥95 (%), Fe2O3 content ≤1.2 (%).

[0040] Polyether polyol, using polyether polyol EP330N, brand: Bluestar Dongda.

[0041] Polybutylene adipate, CAS: 150923-12-9, Mw: 1000, Guangdong Wengjiang Chemical Reagent Co., Ltd.

[0042] Polydimethylsiloxane, product number: U31101115, Shandong Yousuo Chemical Technology Co., Ltd.

[0043] 4,4'-Benzaldione (6-methyl-m-phenylene) tetraisocyanate, CAS No.: 28886-07-9.

[0044] 2,2-Bis(4-isocyanatophenyl)hexafluoropropane, CAS number: 10224-18-7.

[0045] 4,4',4"-Triphenylmethane triisocyanate, CAS No.: 2422-91-5. The remaining raw materials in the embodiments of the present invention and the comparative examples are all commercially available products.

[0046] The design idea of ​​the present invention is to prepare an efficient and stable biological deodorant for odor control in environments such as garbage stations. By carefully selecting and mixing a variety of beneficial microorganisms, a composite microbial community is formed, and these microorganisms work together under specific fermentation conditions to enhance the deodorization effect. At the same time, the invention adopts a special method for preparing a microbial carrier, which combines high-temperature roasted natural mineral materials and high-performance polyurethane foam to provide a suitable growth environment and protection for microorganisms, thereby improving the adhesion, durability and biological activity of the deodorant. In addition, by optimizing the composition of the fermented material and the treatment process of the microbial carrier, the performance of the deodorant is further improved, so that it has better degradation efficiency and long-term stability in practical applications. This strategy of comprehensively considering microbial activity and carrier performance provides an innovative solution for the development of new environmentally friendly deodorization technologies.

[0047] Example 1 A method for preparing a biological deodorant for a garbage station is as follows: Step 1, brewer's yeast, Bacillus amyloliquefaciens, Enterococcus faecalis, and Lactobacillus plantarum are mixed in a mass ratio of 3:1:1:2 to form a composite microbial community; the composite microbial community is introduced into a tryptic soy bean liquid culture medium TSB and cultured in a 32° C. incubator for 72 hours to promote its growth and reproduction. After the culture process is completed, a composite bacterial agent stock solution is obtained; Step 2, dry-heat sterilize the microbial carrier at 120°C for 3 hours, immerse the sterilized microbial carrier in the composite bacterial agent stock solution obtained in step 1 for 12 hours, the mass ratio of the microbial carrier to the composite bacterial agent stock solution obtained in step 1 is 1:3, take out and drain in the air until there are no water droplets on the surface, to obtain a biological deodorant for garbage stations.

[0048] The preparation method of the microbial carrier is as follows: S1, weighing 0.8 kg of kaolin, 0.3 kg of zeolite and 0.1 kg of diatomaceous earth, mixing these raw materials with 3 kg of water to form a uniform slurry, and making the slurry into wet spherical particles with a particle size of 0.3 cm, then placing these wet spherical particles in an environment of 20° C. for aging for 12 hours to promote uniform distribution of moisture, and the aged particles enter a high-temperature calcination stage, the calcination temperature is controlled at 800° C., and the duration is 3 hours to form a pretreated carrier; S2, weighing 70kg of polyether polyol EP330N, 15kg of poly(1,4-butylene adipate), 7kg of water, 2kg of polydimethylsiloxane, 0.1kg of stannous octoate, 0.5kg of 2-hydroxyethyltriethylenediamine, and 40kg of 1,2,3-trivinylbenzene, and fully mixing these raw materials in a reactor, maintaining the temperature in the reactor at 40° C. to promote the interaction between the raw materials until a uniform mixture is formed, adding the pretreated carrier prepared in step S1, and continuing to stir to ensure that it is evenly dispersed in the mixture to form a post-processed product; S3, weighing 120 kg of 4,4'-benzylidene di(6-methyl-m-phenylene) tetraisocyanate and 0.1 kg of benzoyl peroxide, and mixing the two raw materials in a reaction kettle to ensure that the initiator is evenly dispersed to form a treating agent; S4. The post-treated product obtained in step S2 and the treatment agent prepared in step S3 are fully mixed in a reactor to ensure that the two are completely mixed. The mixed material is poured into a mold and subjected to a foaming reaction at room temperature for 15 hours. After the foaming is completed, the material is soaked in alkali solution. The alkali solution soaking method is to immerse the material in the alkali solution. The mass ratio of the material to the alkali solution is 1:10. The soaking treatment is for 24 hours. The composition of the alkali solution is: a mixed solution of 10 mol / L sodium hydroxide aqueous solution and ethylene glycol in a volume ratio of 4:3; then the material is washed with distilled water for 3 times, and the amount of distilled water used each time is 3 times the weight of the material to obtain a microbial carrier.

[0049] Example 2 The preparation method of a biological deodorant for garbage stations is basically the same as that in Example 1, the only difference being the preparation method of the microbial carrier.

[0050] The preparation method of the microbial carrier is as follows: S1, weighing 0.8 kg of kaolin, 0.3 kg of zeolite and 0.1 kg of diatomaceous earth, mixing these raw materials with 3 kg of water to form a uniform slurry, and making the slurry into wet spherical particles with a particle size of 0.3 cm, then placing these wet spherical particles in an environment of 20° C. for aging for 12 hours to promote uniform distribution of moisture, and the aged particles enter a high-temperature calcination stage, the calcination temperature is controlled at 800° C., and the duration is 3 hours to form a pretreated carrier; S2, weighing 70kg of polyether polyol EP330N, 15kg of poly(1,4-butylene adipate), 7kg of water, 2kg of polydimethylsiloxane, 0.1kg of stannous octoate, 0.5kg of 2-hydroxyethyltriethylenediamine, and 40kg of α-methylstyrene, and fully mixing these raw materials in a reactor, maintaining the temperature in the reactor at 40° C. to promote the interaction between the raw materials until a uniform mixture is formed, adding the pretreated carrier prepared in step S1, and continuing to stir to ensure that it is evenly dispersed in the mixture to form a post-treated product; S3, weighing 120 kg of 4,4'-benzylidene di(6-methyl-m-phenylene) tetraisocyanate and 0.1 kg of benzoyl peroxide, and mixing the two raw materials in a reaction kettle to ensure that the initiator is evenly dispersed to form a treating agent; S4. The post-treated product obtained in step S2 and the treatment agent prepared in step S3 are fully mixed in a reactor to ensure that the two are completely mixed. The mixed material is poured into a mold and subjected to a foaming reaction at room temperature for 15 hours. After the foaming is completed, the material is soaked in alkali solution. The alkali solution soaking method is to immerse the material in the alkali solution. The mass ratio of the material to the alkali solution is 1:10. The soaking treatment is for 24 hours. The composition of the alkali solution is: a mixed solution of 10 mol / L sodium hydroxide aqueous solution and ethylene glycol in a volume ratio of 4:3; then the material is washed with distilled water for 3 times, and the amount of distilled water used each time is 3 times the weight of the material to obtain a microbial carrier.

[0051] Example 3 The preparation method of a biological deodorant for garbage stations is basically the same as that in Example 1, the only difference being the preparation method of the microbial carrier.

[0052] The preparation method of the microbial carrier is as follows: S1, weighing 0.8 kg of kaolin, 0.3 kg of zeolite and 0.1 kg of diatomaceous earth, mixing these raw materials with 3 kg of water to form a uniform slurry, and making the slurry into wet spherical particles with a particle size of 0.3 cm, then placing these wet spherical particles in an environment of 20° C. for aging for 12 hours to promote uniform distribution of moisture, and the aged particles enter a high-temperature calcination stage, the calcination temperature is controlled at 800° C., and the duration is 3 hours to form a pretreated carrier; S2, weighing 70kg of polyether polyol EP330N, 15kg of poly(1,4-butylene adipate), 7kg of water, 2kg of polydimethylsiloxane, 0.1kg of stannous octoate, 0.5kg of 2-hydroxyethyltriethylenediamine, and 40kg of m-fluorostyrene, and fully mixing these raw materials in a reactor, maintaining the temperature in the reactor at 40° C. to promote the interaction between the raw materials until a uniform mixture is formed, adding the pretreated carrier prepared in step S1, and continuing to stir to ensure that it is evenly dispersed in the mixture to form a post-treated product; S3, weighing 120 kg of 4,4'-benzylidene di(6-methyl-m-phenylene) tetraisocyanate and 0.1 kg of benzoyl peroxide, and mixing the two raw materials in a reaction kettle to ensure that the initiator is evenly dispersed to form a treating agent; S4. The post-treated product obtained in step S2 and the treatment agent prepared in step S3 are fully mixed in a reactor to ensure that the two are completely mixed. The mixed material is poured into a mold and subjected to a foaming reaction at room temperature for 15 hours. After the foaming is completed, the material is soaked in alkali solution. The alkali solution soaking method is to immerse the material in the alkali solution. The mass ratio of the material to the alkali solution is 1:10. The soaking treatment is for 24 hours. The composition of the alkali solution is: a mixed solution of 10 mol / L sodium hydroxide aqueous solution and ethylene glycol in a volume ratio of 4:3; then the material is washed with distilled water for 3 times, and the amount of distilled water used each time is 3 times the weight of the material to obtain a microbial carrier.

[0053] Example 4 The preparation method of a biological deodorant for garbage stations is basically the same as that in Example 1, the only difference being the preparation method of the microbial carrier.

[0054] The preparation method of the microbial carrier is as follows: S1, weighing 0.8 kg of kaolin, 0.3 kg of zeolite and 0.1 kg of diatomaceous earth, mixing these raw materials with 3 kg of water to form a uniform slurry, and making the slurry into wet spherical particles with a particle size of 0.3 cm, then placing these wet spherical particles in an environment of 20° C. for aging for 12 hours to promote uniform distribution of moisture, and the aged particles enter a high-temperature calcination stage, the calcination temperature is controlled at 800° C., and the duration is 3 hours to form a pretreated carrier; S2, weighing 70kg of polyether polyol EP330N, 15kg of poly(1,4-butylene adipate), 7kg of water, 2kg of polydimethylsiloxane, 0.1kg of stannous octoate, 0.5kg of 2-hydroxyethyltriethylenediamine, and 40kg of 1,2,3-trivinylbenzene, and fully mixing these raw materials in a reactor, maintaining the temperature in the reactor at 40° C. to promote the interaction between the raw materials until a uniform mixture is formed, adding the pretreated carrier prepared in step S1, and continuing to stir to ensure that it is evenly dispersed in the mixture to form a post-processed product; S3, weighing 120 kg of 2,2-bis(4-isocyanatophenyl)hexafluoropropane and 0.1 kg of benzoyl peroxide, and mixing the two raw materials in a reaction kettle to ensure that the initiator is evenly dispersed to form a treating agent; S4. The post-treated product obtained in step S2 and the treatment agent prepared in step S3 are fully mixed in a reactor to ensure that the two are completely mixed. The mixed material is poured into a mold and subjected to a foaming reaction at room temperature for 15 hours. After the foaming is completed, the material is soaked in alkali solution. The alkali solution soaking method is to immerse the material in the alkali solution. The mass ratio of the material to the alkali solution is 1:10. The soaking treatment is for 24 hours. The composition of the alkali solution is: a mixed solution of 10 mol / L sodium hydroxide aqueous solution and ethylene glycol in a volume ratio of 4:3; then the material is washed with distilled water for 3 times, and the amount of distilled water used each time is 3 times the weight of the material to obtain a microbial carrier.

[0055] Example 5 The preparation method of a biological deodorant for garbage stations is basically the same as that in Example 1, the only difference being the preparation method of the microbial carrier.

[0056] The preparation method of the microbial carrier is as follows: S1, weighing 0.8 kg of kaolin, 0.3 kg of zeolite and 0.1 kg of diatomaceous earth, mixing these raw materials with 3 kg of water to form a uniform slurry, and making the slurry into wet spherical particles with a particle size of 0.3 cm, then placing these wet spherical particles in an environment of 20° C. for aging for 12 hours to promote uniform distribution of moisture, and the aged particles enter a high-temperature calcination stage, the calcination temperature is controlled at 800° C., and the duration is 3 hours to form a pretreated carrier; S2, weighing 70kg of polyether polyol EP330N, 15kg of poly(1,4-butylene adipate), 7kg of water, 2kg of polydimethylsiloxane, 0.1kg of stannous octoate, 0.5kg of 2-hydroxyethyltriethylenediamine, and 40kg of 1,2,3-trivinylbenzene, and fully mixing these raw materials in a reactor, maintaining the temperature in the reactor at 40° C. to promote the interaction between the raw materials until a uniform mixture is formed, adding the pretreated carrier prepared in step S1, and continuing to stir to ensure that it is evenly dispersed in the mixture to form a post-processed product; S3, weighing 120 kg of 4,4',4"-triphenylmethane triisocyanate and 0.1 kg of benzoyl peroxide, and mixing the two raw materials in a reactor to ensure that the initiator is evenly dispersed to form a treating agent; S4. The post-treated product obtained in step S2 and the treatment agent prepared in step S3 are fully mixed in a reactor to ensure that the two are completely mixed. The mixed material is poured into a mold and subjected to a foaming reaction at room temperature for 15 hours. After the foaming is completed, the material is soaked in alkali solution. The alkali solution soaking method is to immerse the material in the alkali solution. The mass ratio of the material to the alkali solution is 1:10. The soaking treatment is for 24 hours. The composition of the alkali solution is: a mixed solution of 10 mol / L sodium hydroxide aqueous solution and ethylene glycol in a volume ratio of 4:3; then the material is washed with distilled water for 3 times, and the amount of distilled water used each time is 3 times the weight of the material to obtain a microbial carrier.

[0057] Comparative Example 1 The preparation method of a biological deodorant for garbage stations is basically the same as that in Example 1, the only difference being the preparation method of the microbial carrier.

[0058] The preparation method of the microbial carrier is as follows: S1, weighing 0.8 kg of kaolin, 0.3 kg of zeolite and 0.1 kg of diatomaceous earth, mixing these raw materials with 3 kg of water to form a uniform slurry, and making the slurry into wet spherical particles with a particle size of 0.3 cm, then placing these wet spherical particles in an environment of 20° C. for aging for 12 hours to promote uniform distribution of moisture, and the aged particles enter a high-temperature calcination stage, the calcination temperature is controlled at 800° C., and the duration is 3 hours to form a pretreated carrier; S2, weighing 70kg of polyether polyol EP330N, 15kg of poly(1,4-butylene adipate), 7kg of water, 2kg of polydimethylsiloxane, 0.1kg of stannous octoate, 0.5kg of 2-hydroxyethyltriethylenediamine, and 40kg of divinylbenzene, and fully mixing these raw materials in a reactor, maintaining the temperature in the reactor at 40° C. to promote the interaction between the raw materials until a uniform mixture is formed, adding the pretreated carrier prepared in step S1, and continuing to stir to ensure that it is evenly dispersed in the mixture to form a post-processed product; S3, weighing 120 kg of 4,4'-benzylidene di(6-methyl-m-phenylene) tetraisocyanate and 0.1 kg of benzoyl peroxide, and mixing the two raw materials in a reaction kettle to ensure that the initiator is evenly dispersed to form a treating agent; S4. The post-treated product obtained in step S2 and the treatment agent prepared in step S3 are fully mixed in a reactor to ensure that the two are completely mixed. The mixed material is poured into a mold and subjected to a foaming reaction at room temperature for 15 hours. After the foaming is completed, the material is soaked in alkali solution. The alkali solution soaking method is to immerse the material in the alkali solution. The mass ratio of the material to the alkali solution is 1:10. The soaking treatment is for 24 hours. The composition of the alkali solution is: a mixed solution of 10 mol / L sodium hydroxide aqueous solution and ethylene glycol in a volume ratio of 4:3; then the material is washed with distilled water for 3 times, and the amount of distilled water used each time is 3 times the weight of the material to obtain a microbial carrier.

[0059] Comparative Example 2 The preparation method of a biological deodorant for garbage stations is basically the same as that in Example 1, the only difference being the preparation method of the microbial carrier.

[0060] The preparation method of the microbial carrier is as follows: S1, weighing 0.8 kg of kaolin, 0.3 kg of zeolite and 0.1 kg of diatomaceous earth, mixing these raw materials with 3 kg of water to form a uniform slurry, and making the slurry into wet spherical particles with a particle size of 0.3 cm, then placing these wet spherical particles in an environment of 20° C. for aging for 12 hours to promote uniform distribution of moisture, and the aged particles enter a high-temperature calcination stage, the calcination temperature is controlled at 800° C., and the duration is 3 hours to form a pretreated carrier; S2, weighing 70kg of polyether polyol EP330N, 15kg of poly(1,4-butylene adipate), 7kg of water, 2kg of polydimethylsiloxane, 0.1kg of stannous octoate, 0.5kg of 2-hydroxyethyltriethylenediamine, and 40kg of styrene, and fully mixing these raw materials in a reactor, maintaining the temperature in the reactor at 40° C. to promote the interaction between the raw materials until a uniform mixture is formed, adding the pretreated carrier prepared in step S1, and continuing to stir to ensure that it is evenly dispersed in the mixture to form a post-treated product; S3, weighing 120 kg of 4,4'-benzylidene di(6-methyl-m-phenylene) tetraisocyanate and 0.1 kg of benzoyl peroxide, and mixing the two raw materials in a reaction kettle to ensure that the initiator is evenly dispersed to form a treating agent; S4. The post-treated product obtained in step S2 and the treatment agent prepared in step S3 are fully mixed in a reactor to ensure that the two are completely mixed. The mixed material is poured into a mold and subjected to a foaming reaction at room temperature for 15 hours. After the foaming is completed, the material is soaked in alkali solution. The alkali solution soaking method is to immerse the material in the alkali solution. The mass ratio of the material to the alkali solution is 1:10. The soaking treatment is for 24 hours. The composition of the alkali solution is: a mixed solution of 10 mol / L sodium hydroxide aqueous solution and ethylene glycol in a volume ratio of 4:3; then the material is washed with distilled water for 3 times, and the amount of distilled water used each time is 3 times the weight of the material to obtain a microbial carrier.

[0061] Comparative Example 3 The preparation method of a biological deodorant for garbage stations is basically the same as that in Example 1, the only difference being the preparation method of the microbial carrier.

[0062] The preparation method of the microbial carrier is as follows: S1, weighing 0.8 kg of kaolin, 0.3 kg of zeolite and 0.1 kg of diatomaceous earth, mixing these raw materials with 3 kg of water to form a uniform slurry, and making the slurry into wet spherical particles with a particle size of 0.3 cm, then placing these wet spherical particles in an environment of 20° C. for aging for 12 hours to promote uniform distribution of moisture, and the aged particles enter a high-temperature calcination stage, the calcination temperature is controlled at 800° C., and the duration is 3 hours to form a pretreated carrier; S2, weighing 70kg of polyether polyol EP330N, 15kg of poly(1,4-butylene adipate), 7kg of water, 2kg of polydimethylsiloxane, 0.1kg of stannous octoate, 0.5kg of 2-hydroxyethyltriethylenediamine, and 40kg of 1,2,3-trivinylbenzene, and fully mixing these raw materials in a reactor, maintaining the temperature in the reactor at 40° C. to promote the interaction between the raw materials until a uniform mixture is formed, adding the pretreated carrier prepared in step S1, and continuing to stir to ensure that it is evenly dispersed in the mixture to form a post-processed product; S3, weighing 120 kg of diphenylmethane diisocyanate and 0.1 kg of benzoyl peroxide, and mixing the two raw materials in a reactor to ensure that the initiator is evenly dispersed to form a treating agent; S4. The post-treated product obtained in step S2 and the treatment agent prepared in step S3 are fully mixed in a reactor to ensure that the two are completely mixed. The mixed material is poured into a mold and subjected to a foaming reaction at room temperature for 15 hours. After the foaming is completed, the material is soaked in alkali solution. The alkali solution soaking method is to immerse the material in the alkali solution. The mass ratio of the material to the alkali solution is 1:10. The soaking treatment is for 24 hours. The composition of the alkali solution is: a mixed solution of 10 mol / L sodium hydroxide aqueous solution and ethylene glycol in a volume ratio of 4:3; then the material is washed with distilled water for 3 times, and the amount of distilled water used each time is 3 times the weight of the material to obtain a microbial carrier.

[0063] Comparative Example 4 The preparation method of a biological deodorant for garbage stations is basically the same as that in Example 1, the only difference being the preparation method of the microbial carrier.

[0064] The preparation method of the microbial carrier is as follows: S1. Weigh 0.8 kg of kaolin, 0.3 kg of zeolite and 0.1 kg of diatomaceous earth, mix these raw materials with 3 kg of water to form a uniform slurry, and make the slurry into wet spherical particles with a particle size of 0.3 cm. Subsequently, place these wet spherical particles in an environment of 20° C. for aging for 12 hours to promote uniform distribution of moisture. The aged particles enter a high-temperature calcination stage, the calcination temperature is controlled at 800° C., and the duration is 3 hours to obtain a microbial carrier; Comparative Example 5 The preparation method of a biological deodorant for garbage stations is basically the same as that in Example 1, the only difference being the preparation method of the microbial carrier.

[0065] The preparation method of the microbial carrier is as follows: S1, weighing 70kg of polyether polyol EP330N, 15kg of poly(1,4-butylene adipate), 7kg of water, 2kg of polydimethylsiloxane, 0.1kg of stannous octoate, 0.5kg of 2-hydroxyethyltriethylenediamine, and 40kg of 1,2,3-trivinylbenzene, and fully mixing these raw materials in a reactor, maintaining the temperature in the reactor at 40° C. to promote interaction between the raw materials, until a uniform mixture is formed, and continuing stirring to form a post-processed product; S2, weighing 120 kg of 4,4'-benzylidene di(6-methyl-m-phenylene) tetraisocyanate and 0.1 kg of benzoyl peroxide, and mixing the two raw materials in a reaction kettle to ensure that the initiator is evenly dispersed to form a treating agent; S3. The post-treated product obtained in step S1 and the treatment agent prepared in step S2 are fully mixed in a reactor to ensure that the two are completely mixed. The mixed material is poured into a mold and subjected to a foaming reaction at room temperature for 15 hours. After the foaming is completed, the material is soaked in alkali solution. The alkali solution soaking method is to immerse the material in the alkali solution. The mass ratio of the material to the alkali solution is 1:10. The soaking treatment is for 24 hours. The composition of the alkali solution is: a mixed solution of 10 mol / L sodium hydroxide aqueous solution and ethylene glycol in a volume ratio of 4:3; then the material is washed with distilled water for 3 times, and the amount of distilled water used each time is 3 times the weight of the material to obtain a microbial carrier.

[0066] Test Example 1 Deodorization effect test The biological deodorants obtained in Examples 5 and Comparative Examples 1 to 5 were mixed with tap water at a mass ratio of 1:1, and brown sugar of 1 / 2 the mass of the biological deodorant was added, and the mixture was stirred at 200 r / min for 2 h to obtain a preparation. The preparation was sprayed on kitchen waste using a spray pot in a sealed room. 2 g of the preparation containing the microbial deodorant was added to each kilogram of kitchen waste. After 1 hour, the content indicators of NH3 and H2S gases were tested using a HF-900 gas chromatograph, and the degradation rate was calculated. The degradation rate (%) = [(odor gas concentration before deodorization - odor gas concentration after deodorization treatment) / odor gas concentration before deodorization] × 100%. The hydrogen sulfide concentration before treatment was 60 ppm, and the ammonia concentration was 75 ppm.

[0067] The test results are shown in Table 1.

[0068] Table 1

[0069] Test Example 2 The long-term stability of the biological deodorant was evaluated. Specifically, the biological deodorant samples prepared in Examples 1 to 5 and Comparative Examples 1 to 5 were stored at 30°C for 40 days to simulate the accelerated aging process of the microbial strains. Subsequently, 1 gram of the biological agent was taken from each sample and placed in a 250 ml conical flask containing sterile glass beads and 100 ml of PBS buffer. These conical flasks were placed on a constant temperature magnetic stirrer with a stirring time of 2 hours to ensure uniform dispersion of the agent. After stirring, the samples were subjected to a series of gradient dilutions and further diluted with sterile water. The diluted samples were evenly spread on specific culture media, including lactobacillus culture media, bacillus culture media and saccharomyces cerevisiae culture media, to promote the growth of the corresponding microorganisms. These culture media were then incubated at 37°C for 28 hours. After the incubation period, the number of colonies on the culture media was counted to evaluate the survival of each active strain in the composite microbial agent after being stored at 30°C for 40 days.

[0070] The test results are shown in Table 2.

[0071] Table 2

[0072] It can be seen from the data in Tables 1 and 2 that the biological deodorant for garbage stations prepared in Example 1 has the best deodorizing effect and long-term stability.

[0073] In Example 1 of the present invention, 1,2,3-trivinylbenzene is used as an olefin monomer, which has three vinyl functional groups, which enables it to provide more cross-linking points in the polymerization reaction, thereby enhancing the mechanical strength and structural stability of the final microbial carrier. In addition, the high reactivity of 1,2,3-trivinylbenzene helps to increase the cross-linking density of the polymer, which is beneficial for improving the porosity and adsorption properties of the microbial carrier. These characteristics make the microbial carrier prepared with 1,2,3-trivinylbenzene as raw material perform better in deodorization effect and long-term stability. In contrast, although other olefin monomers such as α-methylstyrene, m-fluorostyrene, divinylbenzene and styrene can also participate in the polymerization reaction, due to their small number of vinyl functional groups, they are not as effective as 1,2,3-trivinylbenzene in forming a highly cross-linked polymer network.

[0074] In Example 1 of the present invention, 4,4'-benzylidene di(6-methyl-m-phenylene) tetraisocyanate is used as the isocyanate raw material, and its molecule contains four isocyanate groups (-NCO), which enables it to form more crosslinking points in the polymerization reaction, thereby enhancing the network structure and overall stability of the polymer. In contrast, although 2,2-bis(4-isocyanatophenyl)hexafluoropropane in Example 4 and 4,4',4"-triphenylmethane triisocyanate in Example 5 also have good polymerization properties, due to the small number of their functional groups, the crosslinking density is relatively low, which in turn affects the mechanical strength and durability of the final product. The diphenylmethane diisocyanate used in Comparative Example 3 also has similar problems. Therefore, the high functionality and strong crosslinking ability of 4,4'-benzylidene di(6-methyl-m-phenylene) tetraisocyanate make the biological deodorant of Example 1 more superior in terms of deodorization effect and long-term stability.

[0075] In Example 1 of the present invention, by adopting a complete microbial carrier, that is, combining the steps of natural mineral materials and chemical synthetic materials, the prepared biological deodorant for garbage stations exhibits excellent deodorization effect and long-term stability. The combination of natural mineral materials and chemical synthetic materials produces a significant synergistic effect, wherein natural mineral materials such as kaolin, zeolite and diatomaceous earth provide stable attachment points and suitable growth environments for microorganisms due to their unique pore structures and surface properties, thereby enhancing the immobilization effect of microorganisms. Chemical synthetic materials, such as polyether polyols and isocyanates, form carriers with specific porosity and mechanical strength by precisely controlling the foaming and curing processes, which not only protects the microorganisms from environmental stress, but also improves the durability and biocompatibility of the carrier. This combination utilizes the eco-friendliness of natural materials and the designability of synthetic materials to achieve a more efficient and lasting deodorization effect, while ensuring the long-term stability and high efficiency of the biological deodorant. In addition, 1,2,3-trivinylbenzene as an active monomer, its ternary copolymerization characteristics help to form a more stable polymer network, which may react with multiple isocyanate groups in 4,4'-benzylidene di(6-methyl-m-phenylene) tetraisocyanate to form a highly cross-linked polyurethane foam structure. This structure not only enhances the mechanical strength of the carrier, but also may improve its ability to fix microorganisms, providing better protection for microorganisms, thereby maintaining a high survival rate of active strains during the simulated accelerated aging process, thereby improving the long-term stability and effect of the deodorant.

Claims

1. A method for preparing a biological deodorant for a garbage station, characterized in that: By weight: Step 1, Saccharomyces cerevisiae, Bacillus amyloliquefaciens, Enterococcus faecalis, and Lactobacillus plantarum are mixed to form a composite microbial community; the composite microbial community is introduced into a tryptic soy bean liquid culture medium TSB for cultivation to promote its growth and reproduction, and after the cultivation process is completed, a composite bacterial agent stock solution is obtained; Step 2: sterilize the microbial carrier by dry heat, immerse the sterilized microbial carrier into the composite bacterial agent stock solution obtained in step 1, take out and drain in the air until there are no water droplets on the surface, and obtain a biological deodorant for garbage stations.

2. The method for preparing a biological deodorant for a garbage station according to claim 1, characterized in that: The brewer's yeast, Bacillus amyloliquefaciens, Enterococcus faecalis and Lactobacillus plantarum are mixed in a mass ratio of 2-4:0.5-2:0.5-2:1-3.

3. The method for preparing a biological deodorant for a garbage station according to claim 1, characterized in that: The composite microbial community is introduced into tryptic soy bean liquid culture medium TSB and cultured in a constant temperature box at 30-35° C. for 48-96 hours.

4. The method for preparing a biological deodorant for a garbage station according to claim 1, characterized in that: The microbial carrier is dry-heat sterilized at 100-150° C. for 1-5 hours.

5. The method for preparing a biological deodorant for a garbage station according to claim 1, characterized in that: The mass ratio of the microbial carrier to the composite bacterial agent stock solution obtained in step 1 is 0.5-2:2-4.

6. The method for preparing a biological deodorant for a garbage station according to claim 1, characterized in that: The microbial carrier is immersed in the composite bacterial agent stock solution obtained in step 1 for 5 to 24 hours.

7. The method for preparing a biological deodorant for a garbage station according to claim 1, characterized in that: The preparation method of the microbial carrier is as follows, in parts by weight: S1, weighing 0.5-1 parts of kaolin, 0.2-0.4 parts of zeolite and 0.05-0.2 parts of diatomaceous earth, mixing these raw materials with 2-4 parts of water to form a uniform slurry, making the slurry into wet spherical particles with a particle size of 0.2-0.5 cm, then placing these wet spherical particles in an environment of 15-25° C. for 5-24 hours to promote uniform distribution of moisture, and the aged particles enter a high-temperature calcination stage for calcination to form a pretreated carrier; S2, weighing 60-80 parts of polyether polyol, 10-20 parts of poly(1,4-butylene adipate), 5-10 parts of water, 1-3 parts of polydimethylsiloxane, 0.05-0.2 parts of stannous octoate, 0.4-0.6 parts of 2-hydroxyethyltriethylenediamine, and 30-50 parts of 1,2,3-trivinylbenzene, and fully mixing these raw materials in a reactor, maintaining the temperature in the reactor at 35-50° C. to promote interaction between the raw materials until a uniform mixture is formed, adding the pretreated carrier prepared in step S1, and continuing to stir to ensure that it is evenly dispersed in the mixture to form a post-treated product; S3, weighing 100-150 parts of 4,4'-benzylidene di(6-methyl-m-phenylene) tetraisocyanate and 0.05-0.2 parts of benzoyl peroxide, and mixing the two raw materials in a reaction kettle to ensure that the initiator is evenly dispersed to form a treating agent; S4. The post-treated product obtained in step S2 and the treatment agent prepared in step S3 are fully mixed in a reactor to ensure that the two are completely mixed. The mixed material is poured into a mold and subjected to a foaming reaction at room temperature for 10 to 20 hours. After the foaming is completed, the material is soaked in alkaline solution and then washed with water for 1 to 3 times to obtain a microbial carrier.

8. The method for preparing a biological deodorant for a garbage station according to claim 7, characterized in that: In the high temperature calcination stage, the calcination temperature is controlled at 700-900° C. and the duration is 2-4 hours.

9. The method for preparing a biological deodorant for a garbage station according to claim 7, characterized in that: The alkali solution soaking method is to immerse the material in the alkali solution, the mass ratio of the material to the alkali solution is 0.5-2:8-12, and the soaking treatment is 12-48 hours. The composition of the alkali solution is: 8-12 mol / L sodium hydroxide aqueous solution and ethylene glycol are prepared into a mixed solution in a volume ratio of 3-5:2-4.

10. A biological deodorant for a garbage station, characterized in that: Prepared by the preparation method according to any one of claims 1 to 9.

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