A handheld strip detection device

Abstract

The present invention provides a handheld strip detection device, which can detect two types of strips, namely immunofluorescence chromatography strips and colloidal gold strips. The device housing integrates three main parts: a mobile positioning part, a photoelectric detection part, and a circuit part, overcoming the technical problems existing in traditional strip detection methods, such as the overly large device volume, excessively high cost, and the dependence of detection results on visual judgment. The device housing is set in a shape suitable for holding, with a small volume. The user only needs to manually push the strip into the device interior, and the detection can be completed by the collaborative work of the mobile positioning part and the photoelectric detection part. The detection result can also be intuitively displayed on the display screen, and an accurate detection result can be obtained immediately, improving the detection speed and accuracy and reducing the cost. In addition, a sliding cover provided on the outer surface of the device housing, in cooperation with a replaceable optical module, realizes the general detection of immunofluorescence chromatography strips labeled with different fluorescent substances, and can meet the requirements of strip compatibility in clinical detection.

Description

Technical Field

[0001] The present invention relates to the field of detection instruments, and particularly to a device for portable, compatible, and light-source replaceable detection of two types of strip, namely immunofluorescence chromatography strip and colloidal gold strip. Background Art

[0002] In modern medicine, Point-of-Care Testing (POCT) technology occupies an important position in clinical diagnosis because it can quickly provide test results. As common POCT tools, immunofluorescence chromatography strips and colloidal gold strips are widely used for rapid detection of specific markers in biological samples such as blood and urine. Generally, both types of strips are provided with a test line (T line) and a control line (C line) for capturing and detecting the analyte and verifying the effectiveness of the detection process. Specifically, the immunofluorescence chromatography strip is a combination of immunochromatography technology and fluorescence labeling technology. Using a fluorescent substance as a label, quantitative or qualitative judgment is made by detecting the intensity of the fluorescence signal formed by the fluorescent complex fixed by the captured antibody on the test line. The colloidal gold strip uses gold nanoparticles as a label and makes a qualitative judgment by visually observing the color change (such as red) on the test line.

[0003] The peak ratio generally refers to the ratio between the signal intensity on the test line (T line) and the signal intensity on the control line (C line). In strip detection, detecting the "peak ratio" is a key technical indicator aimed at improving the accuracy and reliability of detection results. By calculating this ratio, the signal intensity fluctuations caused by changes in the external environment (such as light intensity, temperature, etc.) and individual differences of operators can be effectively corrected. In addition, the peak ratio can also help quantify the detection results, enabling even weak signal changes to be accurately captured. This not only improves the detection sensitivity but also facilitates the standardization of detection results, thereby reducing the error of subjective judgment and strengthening the quality control of the detection process. However, when detecting immunofluorescence chromatography strips, the position of the card slot entering the instrument is usually judged by the time judgment method to determine the position of the strip during the detection process. The principle of the time judgment method is based on the fact that the time for each advancement of the card slot is fixed, and the position of the card slot is judged by a preset fixed time interval. The specific steps include: presetting the time interval required for each advancement of the card slot, manually or mechanically advancing the card slot, using a timer to record the time for advancing the card slot, and finally judging the position of the card slot by the recorded time interval. This time judgment method does not require additional sensors and has a low cost. However, since the advancing speed of the card slot may change due to uncertain reasons such as human or mechanical factors, relying solely on time to judge the position will cause the fluorescence curve to shift, thus affecting the accuracy of the detection results. At the same time, when the advancing speed is unstable, the time judgment method cannot provide accurate position feedback, resulting in changes in the distance between the T line (test line) and the C line (control line) on the strip, and further leading to incorrect peak seeking and peak ratio calculation, further affecting the accuracy and consistency of the detection results. In addition, to ensure the accuracy of detection, strict control of the advancing speed is required, which will also increase the complexity and difficulty of operation and thus reduce the reliability of detection. The traditional colloidal gold strip judges the detection results by the naked eye, which is extremely vulnerable to environmental light and personal subjective factors, thus having a great potential for error or misjudgment risks.

[0004] There are also some drawbacks in the detection instruments for these two types of strip. First of all, currently, the reading devices for achieving compatible detection of the two types of strip are usually large in size and not suitable for mobile medical treatment or home use. Secondly, the reading devices for achieving compatible detection of the two types of strip, due to the need to integrate the functions of collecting and processing two different signals and the relatively complex layout of functional modules, are relatively high in price, which limits their application in a wider range. Finally, the operation of some devices is relatively complex and requires certain training to be accurately used, which is also a major challenge. These drawbacks limit the popularity of traditional strip detection technology in practical applications, especially in areas with limited resources. Therefore, it is imperative to develop a more portable, economical, and easy-to-operate device or apparatus for the compatibility detection of the two types of strip, so as to solve the problems existing in the prior art such as the over-large size, high cost, and complex operation of the device. In addition, the common fluorescent markers on immunofluorescence chromatography strip include fluorescein, quantum dots, and upconversion nanoparticles. Among them, fluorescein-based fluorescent markers require a light source of ultraviolet light at 365 nm with a relatively short wavelength to be excited. The excitation light source range configured in common fluorescence detectors is generally between 300 nm and 370 nm, which can be used for the detection of corresponding immunofluorescence chromatography strip. However, when the fluorescent markers are quantum dots and upconversion nanoparticles, they cannot be used for detection: quantum dots require a relatively wide range of excitation light wavelengths, including the wavelength range of visible light, while upconversion nanoparticles require a long-wavelength excitation light source, such as near-infrared light, and the common excitation wavelengths include 808 nm, 980 nm, etc. If the excitation light required by the immunofluorescence chromatography strip is within the visible light range, how to achieve the compatible detection with colloidal gold strip; if the immunofluorescence chromatography strip requires a shorter or longer wavelength of excitation light, how to achieve the best detection; therefore, it is also particularly important to develop a more portable, economical, and easy-to-operate strip detection device to meet the diverse needs of immunofluorescence chromatography strip. Summary of the Invention

[0005] The object of the present invention is to overcome the defects of the existing strip detection technology, and provide a handheld strip detection device that is convenient to hold and carry, has a simple structure, a small size, a low cost, a simple operation, a short detection time, flexible use, and high detection accuracy, and can be used for the compatibility detection of different types of strip.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] In a first aspect, the present invention provides a handheld strip detection device capable of simultaneously detecting two types of strips, namely immunofluorescence chromatography strips and colloidal gold strips, for compatibility and portable detection. The housing of the device is set in a shape suitable for holding, and is composed of an upper housing part and a lower housing part. The functional components provided on the outer surface and inside of the housing of the device include a moving and positioning part, a photoelectric detection part, and a circuit part. A display screen and a switch for realizing human-machine interaction are also provided on the outer surface of the housing of the device; the moving and positioning part is used to control the smooth pushing of the strip into the device for detection; the photoelectric detection part is used to emit light and detect the collected optical signal on the strip; the circuit part is used to provide power supply and data transmission for the device; the moving and positioning part includes a card slot for placing the strip, a linear guide rail and a slider for providing a fixed sliding track, and an encoder for ensuring stable sliding movement and accurate alignment; the photoelectric detection part is used to detect the optical signal on the strip, including an optical module, an optical module shielding case, and an optical module fixing block. The optical module is installed in the optical module shielding case, and the optical module shielding case is fixed on the optical module fixing block; two optical paths are formed in the optical module, namely an emission optical path and a reception optical path. The light source is arranged in the light source fixing groove, and the photoelectric sensor is arranged in the photoelectric sensor fixing groove. The emission optical path is used to irradiate the light emitted by the light source from the light source fixing groove onto the strip, and the reception optical path is used to emit the optical signal on the strip from the light exit hole to the photoelectric sensor fixing groove, and is collected by the photoelectric sensor fixed in the photoelectric sensor fixing groove and used for detection; the circuit part includes a main control board, a battery, a power supply board, and a power interface capable of realizing a dual power supply mode.

[0008] According to a preferred embodiment of the present invention, the moving and positioning part further includes an optical coupler, a slider, a damping rack, a rotating shaft, a code disk, a gear, and related fixing structures for providing precise control and smooth movement for the linear guide rail. The optical coupler is used to ensure the accuracy of signal transmission, the slider is used to ensure the smoothness and stability of the movement of the linear guide rail, the damping rack increases the damping coefficient of the slider, ensuring the stability of signal acquisition, and the coordinated cooperation of the rotating shaft, the code disk, and the gear ensures the stable connection and accurate alignment of the moving components.

[0009] Preferably, the fixing structures include an optical coupler fixing block, a right-turn bearing seat, a left-turn bearing seat, a rotating bearing seat fixing block, a damping rack fixing block, and an encoder fixing block. Among them, the optical coupler fixing block is used to fix the optical coupler; the code disk and the gear are installed on the rotating shaft, the right-turn bearing seat and the left-turn bearing seat are used to fix the rotating shaft, and the optical coupler fixing block and the rotating bearing seat fixing block respectively fix the right-turn bearing seat and the left-turn bearing seat; the damping rack fixing block is used to fix the damping rack; the encoder fixing block is used to fix the encoder.

[0010] According to a preferred embodiment of the present invention, in the optoelectronic detection part, the optical module fixing block includes an upper part of the optical module fixing block and a lower part of the optical module fixing block. The upper part of the optical module fixing block is used to fix the optical module shielding case, the lower part of the optical module fixing block is fixed at the bottom of the lower half of the outer shell, and the upper part and the lower part of the optical module fixing block are fixedly connected.

[0011] Preferably, the upper part and the lower part of the optical module fixing block are fixedly connected to form an inverted "L" shape.

[0012] According to a preferred embodiment of the present invention, in the two optical paths, the emission optical path is formed by a light source, a light source fixing groove, a filter, a metal slit, an emission lens, and a light incident hole; the reception optical path is formed by a light exit hole, a reception lens, a wide-diameter filter, a photoelectric sensor fixing groove, and a photoelectric sensor.

[0013] Preferably, the number of metal slits and emission lenses is set to 1-3.

[0014] Further preferably, the number of metal slits and emission lenses is 2, and the two emission lenses are arranged between the two metal slits.

[0015] According to a preferred embodiment of the present invention, in the optoelectronic detection part, the light source is an LED light source, and the photoelectric sensor is a photodiode.

[0016] Preferably, in the optoelectronic detection part, the LED light source is a white LED and / or a green LED.

[0017] According to a preferred embodiment of the present invention, a slidable sliding cover is provided on the outer surface of the upper half of the outer shell, and the optical module is a replaceable structure.

[0018] According to a preferred embodiment of the present invention, the material of the outer shell of the device can be selected from resin, plastic, aluminum alloy, or carbon fiber.

[0019] Preferably, the color of the outer shell of the device is black, which is used to avoid the interference of external light sources on the detection results to the greatest extent.

[0020] According to a preferred embodiment of the present invention, in the mobile positioning part, a universal card insertion track for smoothly placing both an immunofluorescence chromatography card strip and a colloidal gold card strip is provided on the card slot.

[0021] According to a preferred embodiment of the present invention, the supply current of the light source is controlled by the circuit part.

[0022] Preferably, when detecting the immunofluorescence chromatography card strip, the supply current is preferably controlled to be 90-110 milliamperes; when detecting the colloidal gold card strip, the supply current is preferably controlled to be 4-6 milliamperes.

[0023] According to a preferred embodiment of the present invention, in the photoelectric detection part, a layer of copper foil is attached to the outer surface of the optical module shielding case.

[0024] According to a preferred embodiment of the present invention, in the photoelectric detection part, the filter is preferably a square with a side length of 3-7 mm, and can be a green filter or a red filter.

[0025] Preferably, when the light source is a green LED, a green filter is preferred.

[0026] According to a preferred embodiment of the present invention, in the photoelectric detection part, the filter is preferably fixed in the optical module in a plug-in manner.

[0027] Preferably, when detecting an immunofluorescence chromatography strip, the filter can be not set; when detecting a colloidal gold strip, the filter is plug-in mounted into the optical module.

[0028] According to a preferred embodiment of the present invention, in the photoelectric detection part, the wide-diameter filter is relatively wider than the filter, and is preferably a circle or a semi-circle with a diameter of 7.5-10.5 mm.

[0029] According to a preferred embodiment of the present invention, in the photoelectric detection part, the emission lens and the receiving lens are preferably semi-circles with a diameter of 7.5-10.5 mm.

[0030] According to a preferred embodiment of the present invention, in the photoelectric detection part, the emission lens and the receiving lens are preferably fixed in the optical module in a plug-in manner.

[0031] According to a preferred embodiment of the present invention, the photoelectric sensor is fixed above the optical module through a photoelectric sensor fixing groove.

[0032] According to a preferred embodiment of the present invention, the optical module is an opaque structure.

[0033] According to a preferred embodiment of the present invention, the optical module can switch and control the wavelength of the light emitted by the light source; in the photoelectric detection part, when detecting an immunofluorescence chromatography strip, a white LED is preferably used as the light source; when detecting a colloidal gold strip, a green LED with a wavelength of 515-525 nm is preferably used as the light source.

[0034] According to a preferred embodiment of the present invention, in the circuit part, the battery is a rechargeable battery, and a dual power supply mode of battery power supply or direct power supply of a DC power supply can be realized in cooperation with a power supply interface.

[0035] According to a preferred embodiment of the present invention, in the circuit part, the power supply interface can also be used for data transmission.

[0036] In a second aspect, the present invention claims the application of the above-mentioned handheld strip detection device in the field of strip detection.

[0037] Advantages of the present invention:

[0038] The present invention provides a handheld strip detection device for compatibly and portably detecting two types of strips, namely immunofluorescence chromatography strips and colloidal gold strips. The internal layout of the handheld strip detection device provided by the present invention is compact and can be carried portably by hand. Structurally, the outer shell of the device is set in a shape suitable for holding, and is hermetically composed of the upper half and the lower half of the outer shell, forming a complete enclosed space to protect the internal components from external interference and provide a good hand-held experience. The outer surface of the device's outer shell is provided with a display screen and a switch. The switch can be used to control the display screen or switch the type of strip to be detected through the switch, and control the detection result to be displayed on the display screen to achieve human-computer interaction. During detection, the strip can be manually placed in the card slot, and under the coordinated cooperation of the mobile positioning part, the card slot is slid in and accurately positioned to the position to be detected inside the device for detection. This introduction mechanism for the strip to be detected is simple and easy to use, without complex mechanical structures, and can ensure accurate and stable detection positioning. The device is provided with a mobile positioning part. In order to ensure the accuracy, stability, and precision of the movement of the strip driven by the card slot, a series of related structures are set, such as: especially in the mobile positioning part of the device, an encoder and a code disk are set. The encoder is not only used for precise alignment but also can be used to accurately record the position information of the strip to ensure the position accuracy of the sample during the detection process. The code disk is convenient for later data processing; by setting an optocoupler and its fixing structure, the accuracy of positioning and detection results is ensured; the strip is placed in the card slot, and the card slot is accurately positioned along the track on the linear guide rail through the spring snap female seat at the end of the linear guide rail and the spring snap male head on the slider, ensuring the accuracy of the sliding positioning of the strip. The cooperation between the linear guide rail and the slider further ensures the smoothness and stability of the sliding movement; the mobile positioning part is also provided with structures such as a damping rack, a rotating shaft, and a gear. Among them, the damping rack increases the damping coefficient of the slider, reduces the vibration amplitude and speed of the slider, improves stability and safety, and ensures the stability of signal acquisition.

[0039] In the mobile positioning part of the present invention, an encoder is introduced to replace the traditional time judgment method, and a code disc structure is arranged in cooperation, which can effectively solve the deficiency of the time judgment method in detecting immunofluorescence chromatography strips and avoid the adverse effects of changes in the propulsion speed. Specifically, even if the propulsion speed of the card slot changes, the encoder can still monitor the change in the propulsion position of the card slot in real time and generate a pulse signal, that is, the real-time position of the card slot can still be accurately recorded by the encoder, ensuring the position accuracy of the strip during the detection process, and further ensuring that the distance between the T line (detection line) and the C line (control line) on the strip remains consistent, avoiding the fluorescence curve deviation caused by the change in the propulsion speed, and significantly improving the accuracy, stability and reliability of the detection result. In addition, since the encoder can provide high-precision position feedback and is arranged in cooperation with a code disc, the code disc and the gear are both installed on the rotating shaft, the rotation angle and position of the code disc are accurately measured, the actual position of the card slot is determined by counting the number and direction of the pulse signals, and the physical signal of the movement is converted into an electrical signal, which is convenient for subsequent processing of specific data.

[0040] In the mobile positioning part of the present invention, a linear guide rail is arranged. At the same time, a slider is installed on the linear guide rail, and a card slot is fixed on the slider. Two damping racks are provided. One damping rack is fixed on the damping fixed block, and the other damping rack is fixed on the optical module fixed block. The damping fixed block is further fixed at the bottom of the housing. These optimized structural layouts ensure the coordinated operation of the components of the device and improve the overall stability and reliability.

[0041] The photoelectric detection part of the present invention is responsible for capturing the fluorescence signal on the immunofluorescence chromatography strip or the color change on the colloidal gold strip, so as to output a quantitative or qualitative detection result. The photoelectric detection part is provided with an optical module shielding case, which is used to shield external signal interference and ensure the stability of the photoelectric sensor. The optical module is installed in the optical module shielding case, and the optical module shielding case is fixed on the optical module fixing block. The optical module fixing block is composed of the upper part and the lower part of the optical module fixing block. The lower part of the optical module fixing block is fixed at the bottom of the lower half of the outer shell. The upper part and the lower part of the optical module fixing block are fixedly connected to form an "L" shape rotated 90 degrees clockwise. Such a fixed setting not only ensures that the optical module is located above the strip, but also ensures the effective protection and stable installation of the optical module. The light source fixing groove is used to fix the light source, and the light source is used to emit excitation light signals. The photoelectric sensor fixing groove is used to fix the photoelectric sensor, and the photoelectric sensor is used to receive photoelectric acquisition signals. In the present invention, the optical module is installed in the optical module shielding case, the optical module shielding case is fixed in the optical module fixing block, and the optical module fixing block is fixed at the bottom of the outer shell. Such a structure of layer-by-layer fixing and protection ensures the effective protection and stable installation of the optical module. It is preferable to stick a layer of copper foil on the outer surface of the optical module shielding case, which is beneficial to doubling the shielding of external environmental interference. In the optical module, the supply current of the light source is controlled by the circuit part. The higher the current, the brighter the light source. When the brightness of the light source is too high, the excessive light irradiates the strip. When detecting the immunofluorescence chromatography strip, it will cause the fluorescence signal emitted by the fluorescent substance to reach or approach the saturation state, resulting in a decrease in the change range of the fluorescence signal value. That is, too high brightness is beneficial to reducing the influence of external conditions (such as concentration, temperature, etc.) on the fluorescence signal on the strip and improving the detection accuracy. In addition, compared with the method of adjusting the amplification factor of the adjustment circuit, which relies on complex circuit design and debugging, the method of adjusting the current is simpler, more intuitive and easier to implement.

[0042] The handheld strip detection device provided by the present invention is provided with a slidable sliding cover on the outer surface of the outer shell of the device, and is matched with a replaceable optical module carrying different wavelength light sources. Users can easily replace the optical module, which can not only be compatible with 2 types of strips, namely the immunofluorescence chromatography strip and the colloidal gold strip, but also expand the general detection of immunofluorescence chromatography strips labeled with different fluorescent substances. The outer surface of the upper half of the outer shell of the present invention is provided with a slidable sliding cover, and the optical module is replaceable. The sliding cover and the optical module cooperate with each other. By setting different models of optical modules carrying different light sources, the requirements for the types and replacement of the light sources adapted to different strips are met. Thus, it can not only meet the detection requirements of various immunofluorescence chromatography strips, but also solve the essential difficulties such as complex device structure and too large volume caused by integrating a multi-light source system on a fluorescence detector.

[0043] For the circuit part of the present invention, a high-speed single-chip microcomputer can be preferably used for core control and processing in the main control circuit. The output data is combined with software based on the filtering algorithm of Fourier transform to optimize the data processing process, accelerate the processing speed of detection data, and improve the accuracy, stability, and reliability of detection results. Among them, the Fourier filtering algorithm is based on the principle of Fourier transform, which can decompose the original signal into a series of frequency components, and realize the purification of the signal by filtering out the noise components and retaining the useful signals. Specifically, the Fourier transform converts the signal in the time domain into the frequency domain, allowing the identification and removal of those noise frequency components that do not belong to the real signal. By selecting appropriate filtering parameters, the noise interference can be effectively reduced, and the purity and detection accuracy of the signal can be improved.

[0044] The present invention provides a handheld strip detection device that can simultaneously detect two types of strips, namely immunofluorescence chromatography strips and colloidal gold strips. It includes a compact and handheld shell, which is small in size and convenient to carry. Through the shell of the device, three main parts, namely the mobile positioning part, the photoelectric detection part, and the circuit part, are integrated, overcoming the technical problems existing in the traditional strip detection methods, such as the too large size of the device, too high cost, and the detection results relying on visual judgment by the naked eye. Users only need to manually push the immunofluorescence chromatography strip or the colloidal gold strip into the device, and the detection can be completed with the collaborative work of the mobile positioning part and the photoelectric detection part, which can improve the detection speed and accuracy and reduce the detection cost. In addition, the circuit part provides two power supply modes, and can also intuitively display the detection results on the display screen to immediately obtain accurate strip detection results, meeting the requirements for the compatibility detection of multiple strips in clinical scenarios. Therefore, the handheld strip detection device provided by the present invention has the characteristics of small size, low cost, simple operation, short detection time, flexible use, and high detection accuracy, and is particularly suitable for popularization and application in the POCT fields such as home use and on-site use. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] Figure 1 is a perspective view of the left front side of the handheld strip detection device of the present invention;

[0046] Figure 2 is a top view of the handheld strip detection device of the present invention;

[0047] Figure 3 is a rear view of the bottom of the handheld strip detection device of the present invention;

[0048] Figure 4 is a perspective view of the internal structure of the lower half of the shell of the handheld strip detection device of the present invention;

[0049] Figure 5 is a top view of the internal structure of the lower half of the shell of the handheld strip detection device of the present invention;

[0050] Figure 6 This is a perspective view of the left front side of the mobile positioning part of the handheld card strip detection device of the present invention;

[0051] Figure 7 This is a perspective view of the right front side of the mobile positioning part of the handheld card strip detection device of the present invention;

[0052] Figure 8 It is on Figure 7 The perspective view of the right front side after removing the rotary bearing seat fixing block on the basis;

[0053] Figure 9 This is a perspective view of the enlarged partial structure of the mobile positioning part of the handheld card strip detection device of the present invention;

[0054] Figure 10 This is a perspective view and a top view of the relevant structures between the linear guide rails in the mobile positioning part of the handheld card strip detection device of the present invention;

[0055] Figure 11 This is a perspective view of the photoelectric detection part of the handheld card strip detection device of the present invention;

[0056] Figure 12 This is a perspective view of the optical module in the photoelectric detection part of the handheld card strip detection device of the present invention;

[0057] Figure 13 This is a sectional view and a rear view of the optical module in the photoelectric detection part of the handheld card strip detection device of the present invention;

[0058] Figure 14 This is the operation flow chart for replacing the optical module of the handheld card strip detection device of the present invention;

[0059] Figure 15 This is the detection result diagram of the weakly positive card strip. Among them, A is the detection result diagram of the handheld card strip detection device of the present invention, and B is the detection result diagram of the motor scanning fluorescence analyzer.

[0060] In the figure: 1 - display screen; 2 - upper half of the housing; 3 - switch; 4 - lower half of the housing; 5 - card strip; 6 - card slot; 7 - sliding cover; 8 - power switch; 9 - dual-color indicator light hole; 10 - power interface; 11 - fixing hole for optocoupler fixing block; 12 - fixing hole for optical module fixing block; 13 - fixing hole for encoder fixing block; 14 - shielding case of optical module; 15 - main control board; 16 - optical module; 17 - battery; 18 - power supply board; 19 - optocoupler fixing block; 20 - rotating shaft; 21 - code disk; 22 - encoder; 23 - linear guide; 24 - female seat of spring snap; 25 - dual-color indicator light; 26 - optocoupler; 27 - fixing block for rotating bearing seat; 28 - slider; 29 - damping rack; 30 - left rotating bearing seat; 31 - right rotating bearing seat; 32 - gear; 33 - male head of spring snap; 34 - lower part of optical module fixing block; 35 - upper part of optical module fixing block; 36 - fixing slot for photoelectric sensor; 37 - fixing slot for light source; 38 - emission lens; 39 - fixing hole for optical module; 40 - receiving lens; 41 - light outlet hole; 42 - light inlet hole; 43 - metal slit; 44 - filter; 45 - wide-diameter filter; 46 - left half of optical module; 47 - right half of optical module; 48 - optical module for immunofluorescence chromatography card strip; 49 - optical module for colloidal gold card strip; 50 - fixing block for damping rack; 51 - encoder fixing block. Detailed implementation manners

[0061] The following describes the implementation manners of the present invention in detail with reference to the accompanying drawings. To clearly and completely illustrate the specific exemplary implementation manners of the handheld card strip detection device of the present invention, many specific details are described to comprehensively complete and understand the overall inventive concept of the present invention. However, in other cases, one or more well-known implementation manners can also be implemented without these specific details.

[0062] The present invention claims to protect a handheld card strip detection device. In the present invention, "detection" specifically refers to the detection of the results of antigen or antibody specific immune reactions occurring on immunofluorescence chromatography card strips and colloidal gold card strips, belonging to the field of in vitro diagnostic reagents. The handheld card strip detection device of the present invention belongs to the supporting detection instrument or device for these two types of card strips, namely immunofluorescence chromatography card strips and colloidal gold card strips. Among them, the fluorescence instrument selected for detection comparison is a motor-scanned fluorescence instrument, which is a self-developed product of the applicant's laboratory of the present invention.

[0063] According to the general inventive concept, the present invention provides a handheld strip detection device, including a compact and handheld housing, which is small in size and convenient to carry. By integrating three main parts, namely, a mobile positioning part, a photoelectric detection part, and a circuit part, into the housing of the device, the technical problems existing in the traditional strip detection method, such as the too large size of the device, too high cost, and the detection result depending on the naked-eye judgment, are overcome. The user only needs to manually push an immunofluorescence chromatography strip or a colloidal gold strip into the device, and the detection can be completed by the collaborative work of the mobile positioning part and the photoelectric detection part, which can improve the detection speed and accuracy and reduce the detection cost. In addition, the circuit part provides two power supply modes, and can also intuitively display the detection result on the display screen, so as to obtain an accurate strip detection result immediately, and can meet the requirements of compatibility detection of various strips in the clinical scenario. In addition, for the handheld strip detection device provided by the present invention, by providing a slidable sliding cover on the outer surface of the housing of the device and cooperating with a replaceable optical module carrying different wavelength light sources, the general detection of immunofluorescence chromatography strips labeled with different fluorescent substances is realized.

[0064] The following will further describe in detail the structure and function of the handheld strip detection device of the present invention with reference to the attached Figures 1 - 15 drawings.

[0065] Embodiment 1 Handheld Strip Detection Device

[0066] The handheld strip detection device of the present invention can simultaneously perform compatibility and portable detection on two types of strips, namely immunofluorescence chromatography strips and colloidal gold strips. The housing of the device is set in a shape suitable for holding, and is composed of the upper housing part 2 and the lower housing part 4. The functional components arranged on the outer surface and inside of the device housing include a moving and positioning part, a photoelectric detection part, and a circuit part. A display screen 1, a switch 3, and a slidable sliding cover 7 for realizing human-machine interaction are also arranged on the outer surface of the device housing; the moving and positioning part is used to control the smooth pushing of the strip 5 into the device for detection; the photoelectric detection part is used to emit light and detect the collected optical signal on the strip 5; the circuit part is used to provide power supply and data transmission for the device; the moving and positioning part includes a card slot 6 for placing the strip 5, a linear guide rail 23 and a slider 28 for providing a fixed sliding track, and an encoder 22 for ensuring stable and accurate alignment during sliding movement; the photoelectric detection part is used to detect the optical signal on the strip 5, including an optical module 16, an optical module shielding case 14, and an optical module fixing block. The optical module 16 is a replaceable structure. The optical module 16 is installed in the optical module shielding case 14, and the optical module shielding case 14 is fixed on the optical module fixing block; two optical paths are formed in the optical module 16, namely an emission optical path and a reception optical path. The light source is arranged in the light source fixing groove 37, and the photoelectric sensor is arranged in the photoelectric sensor fixing groove 36. The emission optical path is used to irradiate the light emitted by the light source from the light source fixing groove 37 onto the strip 5, and the reception optical path is used to emit the optical signal on the strip 5 from the light outlet hole 41 to the photoelectric sensor fixing groove 36, and is collected by the photoelectric sensor fixed in the photoelectric sensor fixing groove 36 and used for detection; the circuit part includes a main control board 15, a battery 17, a power supply board 18, and a power interface 10 capable of realizing a dual power supply mode. Among them, the power interface 10 can also be used for data transmission.

[0067] Figure 1 Fig. 4 is a perspective view of the left front side of the handheld strip detection device of the present invention. It can be seen that the overall housing of the device is composed of the upper housing part 2 and the lower housing part 4. These two parts can be tightly fastened together by various detachable methods such as snap fasteners, rabbets and latches, screws, elastic snap fasteners, or slide rails and chutes, which is convenient for later disassembly, replacement, or repair; the material of the housing can be selected from resin, plastic, aluminum alloy, or carbon fiber. Considering the cost, the material is preferably plastic. The color of the device housing is preferably black to avoid interference from external light sources to the detection results to the greatest extent.

[0068] Among them, the upper part of the upper half of the housing 2 is the front of the device, including a display screen 1, a switch 3 and a slidable sliding cover 7; the display screen 1 can be an OLED screen, an LCD screen or a touch screen; the switch 3 is preferably a multi-directional switch, which is used to control the rapid switching of the detection of two types of strip, namely the immunofluorescence chromatography strip and the colloidal gold strip, and to control the display of the detection results on the display screen 1; both the display screen 1 and the switch 3 are fixed on the front of the housing, facing the operator, which is convenient for rapid operation and viewing of the detection results; the sliding cover 7 can be slid off and used to replace the optical module. The lower half of the housing 4 is provided with a push-pull sliding slot 6. After the slot 6 slides out, the strip 5 to be tested can be positioned and placed therein. After the strip 5 is placed, the slot 6 can be manually pushed into the device to perform the detection operation on the strip 5; the slot 6 is provided with a universal strip insertion track that can smoothly place both the immunofluorescence chromatography strip and the colloidal gold strip, ensuring that different strips can be smoothly placed and carried by the slot 6 to the detection position. When starting the detection, the specific type or model of the strip 5 to be tested can be selected through the switch 3. Whether it is an immunofluorescence chromatography strip or a colloidal gold strip, by selecting the correct type or model of the strip, the circuit part of the device can automatically adjust the detection parameters to ensure the accuracy and reliability of the detection results.

[0069] Figure 2 This is a top view of the handheld strip detection device of the present invention, that is, the external view of one side of the top of the device. Figure 2 It can be seen that a power switch 8, a two-color indicator light hole 9 and a power interface 10 are provided on the lower half of the housing 4. Among them, the power switch 8 is used to control the on and off of the power supply of the entire device. Figure 5 Looking at it, the two-color indicator light hole 9 cooperates with the two-color indicator light 25 to indicate the charging and discharging status of the built-in battery module, ensuring that the device is always powered on. The power interface 10 is used to charge the battery module or directly supply power to the device, preferably a USB connector, such as a common Type-C interface.

[0070] Figure 3 The structural layout after rotating the rear view of the bottom of the handheld strip detection device of the present invention clockwise by 90 degrees is shown. The bottom of the device corresponds to the outside of the bottom of the lower half of the housing 4, and mainly has three fixing hole structures, which respectively correspond to the optocoupler fixing block fixing hole 11, the optical module fixing block fixing hole 12 and the encoder fixing block fixing hole 13. Among them, Figure 4 Looking at it, the optocoupler fixing block fixing hole 11 is used to fixedly connect the optocoupler fixing block 19; Figure 11 Looking at it, the optical module fixing block fixing hole 12 is used to fixedly connect the lower part 34 of the optical module fixing block. Figure 8Look, the encoder fixing block fixing holes 13 are used to fixedly connect the encoder fixing block 51; these three fixing holes can preferably be fixedly connected continuously by screws. The encoder 22 can be an optical encoder, an incremental encoder, a magnetic encoder, etc., and the incremental encoder is preferably used in the present invention.

[0071] Figure 4 This is a three-dimensional view (right front side) of the internal structure of the lower half of the housing of the handheld strip detection device of the present invention. The main functional components of the device are shown in this figure. The main functional components include a moving and positioning part, an optical detection part, and a circuit part. These three main functional components are all fixedly arranged on the lower half 4 of the housing. Among them, the moving and positioning part drives the strip 5 to move and be positioned and detected under the optical detection part. Combined with Figures 8 - 9It can be seen that the mobile positioning part includes a rotating shaft 20, a code disk 21, an encoder 22, etc. The code disk 21 is installed on the rotating shaft 20, ensuring the stable connection and precise alignment of related components. With the coordinated work of the encoder 22 and the linear guide 23, the card strip 5 placed on the card slot 6 is manually pushed and slid to be positioned inside the device for detection. The encoder 22 is fixed in the encoder fixing block 51, and the encoder fixing block 51 is further fixed at the bottom of the lower half 4 of the housing through the encoder fixing block fixing hole 13, which is used to ensure the stability of the connection and the precise control of alignment, thus ensuring the stable operation and accurate detection of the device. The photoelectric detection part mainly includes an optical module shielding case 14, an optical module 16, an optocoupler fixing block 19, etc. The optical module 16 is fixed in the optical module shielding case 14, and the optical module shielding case 14 is fixed above the upper part 35 of the optical module fixing block, which is used to shield external signal interference and ensure the stability of the photoelectric sensor. The optocoupler fixing block 19 is fixed on the right side of the device and is used to fix three optocouplers 26. The circuit part integrates the functions of a power supply circuit and a main control circuit, provides a stable power supply for the entire system, is responsible for controlling the operation logic of the entire device, processes the data collected by the photoelectric detection part, and outputs the results. It integrates a microcontroller, a power management chip, a memory, and other necessary electronic components for data processing and result display. The specific components of the circuit part include a main control board 15, a battery 17, a power supply board 18, etc., which are respectively fixed on the left side of the lower half 4 of the housing. Among them, the battery 17 is a rechargeable battery, and the power interface 10 can charge the battery 17. The battery 17 can be a lithium battery, a solar battery, or a dry battery, preferably a lithium battery. The cooperation between the battery 17 and the power interface 10 can achieve a dual power supply mode of battery 17 power supply or direct power supply of a DC power supply. Functionally, the circuit part can also include a dual power supply switching circuit, a power supply circuit, a main control circuit, a photoelectric detection circuit, an amplification circuit, and a constant current drive circuit. Different circuit boards are connected to each other through wires, and preferably fixed connections are made using screws to ensure the stability and reliability of the entire system. Among them, the main control circuit can intelligently switch the detection mode, automatically adjust the detection parameters according to the type of the placed card strip. Through the automatic identification and switching control of the main control circuit, it can not only be compatible with different types of card strips, but also ensure the intelligence, automation, accuracy, and reliability of the detection results. The photoelectric detection circuit can automatically detect information such as the stable connection state and position of all components, thus ensuring the stable operation and accurate detection of the device. The main control circuit can preferably use a high-speed single-chip microcomputer for core control processing. The output data is combined with software built based on a filtering algorithm of Fourier transform to optimize the data processing process, accelerate the processing speed of the detection data, and improve the accuracy, stability, and reliability of the detection results. Figure 4 The connection between related components is preferably fixed by screws. The encoder 22 can be an optoelectronic encoder, a magnetic encoder, or a mechanical encoder.

[0072] Figure 5 It is the structural layout after first removing the upper half 2 of the housing of the handheld strip detection device and the optical module 16, and then rotating the top view of the internal structure of the lower half 4 of the housing clockwise by 90 degrees. Combining Figure 4 It can be seen that there are multiple optocouplers 26, preferably 3 optocouplers 26 are provided. The optocouplers 26 are fixedly installed on the optocoupler fixing block 19. On the one hand, the fixed installation position of the optocoupler 26 needs to be accurately positioned to ensure the accuracy of detection. On the other hand, the optocoupler fixing block 19 has precise dimensions and optocoupler fixing block fixing holes 11 for fixing and supporting the optocoupler 26, which can not only simplify the installation and improve the installation accuracy, but also further ensure that the optocoupler 26 can be accurately installed and positioned according to the predetermined position and angle, which is beneficial to providing stable and reliable detection signals and further improving the accuracy of the detection results. The connection between the optocoupler-related components (optocoupler 26, optocoupler fixing block 19 and optocoupler fixing block fixing hole 11) is preferably fixed by screws. Figure 10 It is a schematic diagram of the connection relationship of the linear guide 23 in the moving and positioning part. Figure 5 and Figure 10 Combining to see: The spring snap female seat 24 is fixedly located at the end of the linear slide rail 23. A mating spring snap male head 33 is installed at the position of the slider 28 corresponding to the spring snap female seat 24. When the slider 28 is pushed to the end, the spring snap female seat 24 and the spring snap male head 33 are latched to ensure the accuracy of the sliding position of the slider 28, thereby ensuring that the strip 5 is carried and slid to the accurate position. In addition, the card slot 6 is fixed on the slider 28. The slider 28 is installed on the linear guide 23, and it is ensured that the slider 28 slides on the linear guide 23. Pushing and pulling the card slot 6 can make the slider 28 smoothly move forward or backward on the linear guide 23. The linear guide 23 is further fixed at the bottom of the lower half 4 of the housing. The coordinated action of these components ensures the smoothness and stability of the sliding. The purpose of setting the relevant structure of the linear guide 23 is to provide a fixed sliding track for the sliding movement. Theoretically, it can be replaced by a ball screw structure or a rack and pinion combination structure and other related moving forms.

[0073] Figure 6 It is a left front three-dimensional view of the relevant structure of the moving and positioning part of the handheld strip detection device of the present invention. Figure 7 It is a right front three-dimensional view of the relevant structure of the moving and positioning part of the handheld strip detection device of the present invention that does not include optocoupler-related components (such as optocoupler 26 and optocoupler fixing block 19). Figure 8 It is in Figure 7 The right front three-dimensional view of the relevant structure of the moving and positioning part after removing the occlusion of the rotary bearing seat fixing block 27 on this basis. Figures 6 - 8 It shows the flexibility and accuracy of the moving and positioning part of the handheld strip detection device of the present invention, specifically reflected in: Combining Figure 10Look, the present invention provides two types of strip cards, namely immunofluorescence chromatography strip cards and colloidal gold strip cards. The strip cards 5 of both types can be commonly used and are inserted into the strip card insertion track slot 6. The slot 6 drives the strip card 5 to smoothly slide on the linear guide rail 23 and reach the designated detection position; combined with Figure 5 Look, the linear guide rail 23 is fixed at the bottom of the lower half part 4 of the housing. The slot 6 is installed on the upper part of the slider 28. The slider 28 slides along the track on the linear guide rail 23 and drives the slot 6 installed thereon to slide along the track. Combined with Figures 7 - 8 Look, the moving and positioning part further provides two symmetrically arranged damping racks 29, two damping rack fixing blocks 50, two gears 32, two bearing seats for rotation (left rotation bearing seat 30, right rotation bearing seat 31) and a bearing seat fixing block 27. The two damping rack fixing blocks 50 are fixed on the side wall of the lower half part 4 of the housing. The two damping racks 29 are respectively fixed on the two damping rack fixing blocks 50. The damping rack 29 increases the damping coefficient of the slider 28, ensuring the stability of signal acquisition. The damping rack 29 is in tooth and contact with the gear 32. Both ends of the rotating shaft 20 pass through and are positioned in the two bearing seats for rotation. At the same time, the two bearing seats for rotation (left rotation bearing seat 30, right rotation bearing seat 31) are connected by the rotating shaft 20. The bearing seat fixing block 27 is located outside the left rotation bearing seat 30. The two bearing seats for rotation are respectively fixed on the bearing seat fixing block 27 and the optocoupler fixing block 19. The bearing seat fixing block 27 is further fixed at the bottom of the lower half part 4 of the housing. Figure 8 It can also be seen in the figure: The encoder 22 is fixed on the encoder fixing block 51. The encoder fixing block 51 is further fixed at the bottom of the lower half part 4 of the housing; Figure 8 and Figure 9 Combined, it can be seen that the code disk 21 and the gear 32 are installed on the rotating shaft 20. The structural layout of the moving and positioning part ensures the coordinated operation among all components, improving the overall stability and reliability.

[0074] Figure 9 Specifically, a perspective view of the enlarged partial structure of the moving and positioning part of the handheld strip card detection device of the present invention is shown. Combined with Figures 7 - 8 and the above description, it can be further seen that both the code disk 21 and the gear 32 are installed on the rotating shaft 20. The rotating shaft 20 is fixed to the two bearing seats for rotation (right rotation bearing seat 31, left rotation bearing seat 30); among them, the right rotation bearing seat 31 is fixed on the optocoupler fixing block 19, and the optocoupler 26 is also fixed on the optocoupler fixing block 19, while the left rotation bearing seat 30 is fixed on the bearing seat fixing block 27. Such a structure ensures the stable connection and accurate alignment of all components, thus guaranteeing the stable operation and accurate detection of the device.

[0075] Figure 11 A perspective view of the photoelectric detection part of the handheld strip card detection device of the present invention is shown. Figure 12 It is a perspective view of the optical module in the photoelectric detection part. Figure 13It is a comparison between a further cross-sectional view and a rear view of the optical module in the optoelectronic detection part. Figures 11 - 13 As can be seen from the combination: Figure 11 It can be seen that the optoelectronic detection part includes an optical module 16, an optical module shielding case 14, and an optical module fixing block. The optical module 16 can adapt to two different types of card strips, namely, immunofluorescence chromatography card strips and colloidal gold card strips, and can not only identify fluorescence signals but also detect color changes caused by colloidal gold particles. The optical module 16 is placed inside the optical module shielding case 14, and then the optical module shielding case 14 is fixed on the optical module fixing block. Among them, the optical module fixing block is composed of the upper part 35 of the optical module fixing block and the lower part 34 of the optical module fixing block. The lower part 34 of the optical module fixing block is fixed at the bottom of the lower half part 4 of the outer shell. The upper part 35 of the optical module fixing block and the lower part 34 of the optical module fixing block are fixedly connected to form an "L" shape rotated 90 degrees clockwise, and then the optical module shielding case 14 is fixed on the upper surface of the upper part 35 of the optical module fixing block. The core purpose of setting the optical module shielding case 14 and the optical module fixing block is to ensure the effective protection and stable installation of the optical module 16. Figure 12 It can be seen that the optical module 16 is mainly composed of the left half part 46 and the right half part 47 of the optical module, aiming to facilitate the installation of two lenses (emitting lens 38, receiving lens 40) and two filter plates (filter plate 44, wide-diameter filter plate 45), and these two parts are fixed through the optical module fixing hole 39. The optical module 16 is also provided with a photoelectric sensor fixing groove 36 and a light source fixing groove 37. Among them, the photoelectric sensor fixing groove 36 is used to fix a photoelectric sensor responsible for receiving optoelectronic acquisition signals, such as a photodiode (PD), a photoresistor, a phototransistor, a photocoupler, or a photovoltaic cell, etc. Preferably, a photodiode with fast response and high sensitivity is selected; the light source fixing groove 37 is used to install a light source. The light source can be a light source that excites the fluorescent substance on the immunofluorescence chromatography card strip to emit light, or a light source that identifies the color change on the colloidal gold card strip, or both types of light sources. Figure 13As can be seen from the rear view of the optical module 16, the bottom of the optical module 16 includes a light incident hole 42 through which light emitted by the light source passes and a light exit hole 41 for receiving light emitted by the card strip 5. The cross-sectional view of the optical module 16 shows two optical paths, namely the optical path for the light source to emit light (hereinafter referred to as the emission optical path) and the optical path for collecting light (hereinafter referred to as the receiving optical path). Specifically, the path of the emission optical path includes that after the light source emits from the light source fixing groove 37, it passes through the filter 44, the metal slit 43 and the emission lens 38 in sequence and then exits from the light incident hole 42. The number of the metal slit 43 and the emission lens 38 on the emission optical path is preferably set to two. The purpose is to filter, focus and shape the emitted light. And preferably, two emission lenses 38 are arranged between two metal slits 43. When the light source in the emission optical path is an LED light source, white LED and / or green LED are preferably used in the LED light source. If the light source is set as a single white LED, the filter 44 can be not configured. This white LED can more widely excite the fluorescent substance and collect the emitted fluorescence. If the light source is set as a single green LED, it can cooperate with the filter 44. After the emitted light passes through the filter 44, it is filtered, focused and shaped by two metal slits 43 and the emission lens 38 and then irradiates the card strip 5 from the light spot formed by the light incident hole 42, which is relatively more suitable for detecting the colloidal gold card strip. The receiving optical path is used to receive the light emitted by the card strip 5. After the light emits from the light exit hole 41, it passes through the receiving lens 40 and the wide-diameter filter 45 in sequence and then enters the photoelectric sensor fixing groove 36 and is captured by the photoelectric sensor arranged in the photoelectric sensor fixing groove 36. Among them, the filter 44 is preferably a square with a side length of 5 mm, which can be a green filter or a red filter. When the light source is a green LED, a green filter is preferably used. The wide-diameter filter 45 is relatively wider than the filter 44, and is preferably a circle or a semi-circle with a diameter of 8 mm. In addition, Figure 13 On the cross-sectional view, it can also be seen that there are optical module fixing holes 39 for fixing and connecting Figure 12 the left half part 46 and the right half part 47 of the optical module shown above.

[0076] Figure 14This is the operation flowchart for replacing the optical module 16 of the handheld strip detection device of the present invention. As mentioned above, the light source of the present invention can be an LED light source, and the light source can also be a tunable laser. By switching the wavelength of the light emitted by the tunable laser through the circuit part, the excitation requirements of different fluorescent substances are met. In addition, according to the main detection requirements and the specific types of fluorescent substances on the strip 5 to be detected, the light source can also be set as an ultraviolet light source (suitable for fluorescein-based fluorescent markers) or a laser light source (suitable for upconversion nanoparticle-based fluorescent markers) applicable to specific immunofluorescence chromatographic strips, etc. The flexibility of the handheld strip detection device of the present invention is also reflected in that the optical module 16 can be configured according to different light sources to select different models of light sources to meet the detection requirements of immunofluorescence chromatographic strips and colloidal gold strips. For example, the optical module 48 for immunofluorescence chromatographic strips is an optical module 16 that can more widely adapt to immunofluorescence chromatographic strips for different fluorescent markers, and is preferably set as an optical module 16 containing a white LED light source; the optical module 49 for colloidal gold strips is an optical module 16 that can maximize the recognition rate and accuracy of the color change caused by the aggregation state of colloidal gold particles, and is preferably set as an optical module 16 containing a green light source. Combining Figure 1 It can be seen that due to the provision of the slidable sliding cover 7 and the optical module 16 being an easily replaceable installation structure, such as snap-on, magnetic attraction, hook-and-hang, slot-in, etc., the sliding cover 7 and the replaceable optical module 16 cooperate to set different models of multiple optical modules 16 according to specific needs, meeting the requirements of different strip adaptation types and easy replacement. Thus, it can not only adapt to the detection requirements of various immunofluorescence chromatographic strips, but also solve the essential difficulties such as complex device structure and excessive volume caused by integrating a multi-light source system on a fluorescence detector.

[0077] In the photoelectric detection part of the present invention, the optical module 16 is replaceably arranged. The replacement operation process of the optical module 16 is as follows: Generally, the sliding cover 7 may be marked with an arrow indicating the sliding direction towards the opening. When replacement is needed, the first step: Manually slide the sliding cover 7 in the direction indicated by the arrow to expose the optical module 16 arranged in the optical module shielding case 14; The second step: Take out the damaged or inapplicable optical module 16 inside according to the detection requirements. In the figure, it is shown that the existing colloidal gold strip optical module 49 is taken out; The third step: Select a new or most applicable optical module 16, position and install it in the optical module shielding case 14, and then manually slide the sliding cover 7 back to its original position and fasten it. For example, in the figure, it is shown that the new or most applicable immunofluorescence chromatography strip optical module 48 is replaced and put in. Thus, the replacement of the optical module 16 is completed. The optical module 16 of the present invention is not only protected by the optical module shielding case 14, but also further sealed and protected by the device housing, which not only ensures the normal operation of the device, but also improves the accuracy and stability of optical detection. The replaceable setting of the optical module 16 meets the diverse needs of users, and the replacement setting makes the operation simple and efficient, meeting the diverse detection tasks of the two different strips, namely immunofluorescence chromatography strips and colloidal gold strips.

[0078] The present invention Figure 14 The replaceable operation of the optical module 16 is shown. To meet the need for diverse strip detections or further reduce costs, the light source in the optical module 16 can also be set as a replaceable structure. For example, different light sources that can be compatible with the detection or are most applicable to the two types of strips, namely immunofluorescence chromatography strips and colloidal gold strips, are set into different models that are size-matched with the light source fixing groove 37. According to the detection requirements or the repair requirements for damaged light sources, only the light source is replaced. In addition, in the present invention, the light source is preferably an LED light source because: If the light source is a green LED, it has a specific wavelength range, can be used to excite fluorescence signals, and can provide more uniform illumination and enhance color contrast. It can not only be used for the detection of immunofluorescence chromatography strips, but also relatively more accurately identify the color changes on the colloidal gold strips. Therefore, it is more suitable for detecting colloidal gold strips. However, if the excitation wavelength does not match, the green LED light source may not be able to effectively excite fluorescence signals and is not applicable to the detection requirements of immunofluorescence chromatography strips; If the light source is a white LED, since the radiation range of the white LED light source includes the wavelength that can excite the fluorescent label, and the spectral range is relatively wide, it can be widely used to excite immunofluorescence chromatography strips with quantum dots as fluorescent labels, and can also meet the detection requirements of colloidal gold strips with relatively lower requirements for the light source.

[0079] Example 2 Detection Application

[0080] According to the general concept of the present invention, a handheld strip detection device is provided, which is a portable device suitable for the POCT field. On the premise of being able to perform compatibility detection on immunofluorescence chromatography strips and colloidal gold strips, it can further improve the detection sensitivity for different strips. During the detection process using the handheld strip detection device of the present invention, relevant data processing such as the peak ratio of the T peak to the C peak, negative and positive judgment, and relevant information such as filtering can be completed and synchronously presented to the user intuitively through the display screen 1.

[0081] Compared with negative and strongly positive strips, the signal intensity of weakly positive strips is low and it is difficult to distinguish from background noise. If the sensitive detection of weakly positive strips can be achieved, it can better reflect the detection sensitivity of the handheld strip detection device of the present invention. Therefore, a weakly positive immunofluorescence chromatography strip is selected, and at the same time, the handheld strip detection device provided by the present invention and a motor-scanning fluorescence analyzer are used for detection and comparison. The detection results are as Figure 15 shown. After the dynamic data of the detection results of the handheld strip detection device of the present invention are output to an intelligent terminal (such as a computer) through the power interface 10, the dynamic curve obtained by fitting the data is as Figure 15 shown in A. It can be seen from the curve graph that there are obvious peaks, and the peak ratio is 0.22, which can be determined as positive. By comparing with the detection results of a common motor-scanning fluorescence analyzer (as Figure 15 shown in B, the peak ratio is 0.25), it can be seen that the detection sensitivity, accuracy, and reliability of the detection results of the handheld strip detection device of the present invention are comparable to those of a common fluorescence detector, and it can distinguish negative, weakly positive, and positive strips portably, intuitively, and accurately.

[0082] The present invention is a portable handheld device for the compatibility detection of two types of strips, namely immunofluorescence chromatography strips and colloidal gold strips. With its compact appearance, simplified structure, and the avoidance of using expensive consumables to reduce costs, it solves the technical problems of large volume and high cost of traditional equipment, ensures the simplicity, flexibility, and convenience of the operation process, and can realize an automated detection process after the strip is placed. It can not only avoid the risk of misjudgment due to the result interpretation being susceptible to environmental light and personal subjective factors, improve the automation and accuracy of the detection, but also greatly promote its popularization and application in the POCT field.

[0083] The specific implementation manners of the present invention have been described in detail, making it easy for those skilled in the art to understand. However, the above specific implementation manners are only used to specifically illustrate the technical solutions or preferred solutions of the present invention in combination with the drawings, rather than an exhaustive limitation of the protection scope of the present invention. According to all the descriptions that have been made public, appropriate modifications or replacements can be made to the details without departing from the essence and scope of the technical solutions of the present invention. The entire scope of the present invention is given by the appended claims and any equivalents thereof.

Claims

1. A handheld card strip detection device, characterized in that: Used for compatibility and portable detection of multiple card strips (5), the shell of the device is arranged in a shape suitable for hand-holding, and is composed of an upper shell part (2) and a lower shell part (4), and the functional components arranged on the outer surface and inside of the shell of the device include a mobile positioning part, a photoelectric detection part and a circuit part, and a display screen (1) and a switch (3) for realizing human-computer interaction are also arranged on the outer surface of the shell of the device; the mobile positioning part is used to control the card strip (5) to be smoothly pushed into the inside of the device for detection; the photoelectric detection part is used to emit light and detect the collected light signal on the card strip (5); and the circuit part is used to provide power supply and data transmission for the device; The movable positioning part comprises a card slot (6) for accommodating a card strip (5), a linear guide rail (23) and a slider (28) for providing a fixed sliding track, an encoder (22) for ensuring stable sliding movement and accurate alignment, and an optical coupler (26) and a related fixing structure for providing precise control and smooth movement for the linear guide rail (23), wherein the optical coupler (26) is used to ensure the accuracy of signal transmission, and the fixing structure comprises an optical coupler fixing block (19), a right-turn bearing seat (31), a left-turn bearing seat (30), a rotating bearing seat fixing block (27), a damping rack fixing block (50), and an encoder fixing block (51), wherein the optical coupler fixing block (19) is used to fix the optical coupler (26); The photoelectric detection part is used to detect the optical signal on the card strip (5), and comprises an optical module (16), an optical module shielding shell (14) and an optical module fixing block, wherein the optical module (16) is installed in the optical module shielding shell (14), and the optical module shielding shell (14) is fixed on the optical module fixing block; two optical paths are formed in the optical module (16), namely an emission optical path and a receiving optical path, the light source is arranged in the light source fixing groove (37), and the photoelectric sensor is arranged in the photoelectric sensor fixing groove (36); the emission optical path is used to irradiate the light emitted by the light source from the light source fixing groove (37) onto the card strip (5), and the receiving optical path is used to emit the optical signal on the card strip (5) from the light exit hole (41) to the photoelectric sensor fixing groove (36), and the optical signal is collected by the photoelectric sensor fixed in the photoelectric sensor fixing groove (36) for detection; The circuit part comprises a main control board (15), a battery (17), a power supply board (18) and a power supply interface (10).

2. The handheld card strip detection device according to claim 1, characterized in that: The moving positioning part also includes a slider (28), a damping rack (29), a rotating shaft (20), a code disc (21), and a gear (32). The slider (28) is used to ensure the smoothness and stability of the movement of the linear guide rail (23). The damping rack (29) increases the damping coefficient of the slider (28) to ensure the stability of signal acquisition. The code disc (21) and the gear (32) are installed on the rotating shaft (20). The coordinated cooperation of the rotating shaft (20), the code disc (21) and the gear (32) ensures the stable connection and precise alignment of the moving components.

3. The handheld card strip detection device according to claim 1, characterized in that: In the photoelectric detection part, the optical module fixing block comprises an optical module fixing block upper part (35) and an optical module fixing block lower part (34), the optical module fixing block upper part (35) is used to fix the optical module shielding shell (14), and the optical module fixing block lower part (34) is fixed to the bottom of the lower half of the housing (4), and the optical module fixing block upper part (35) and the optical module fixing block lower part (34) are fixedly connected.

4. The handheld card strip detection device according to claim 1, characterized in that: The emission light path is formed by a light source, a light source fixing groove (37), a filter (44), a metal slit (43), an emission lens (38) and a light entrance hole (42); and the receiving light path is formed by a light exit hole (41), a receiving lens (40), a wide-diameter filter (45), a photoelectric sensor fixing groove (36) and a photoelectric sensor.

5. The handheld card strip detection device according to claim 1, characterized in that: The light source is an LED light source, and the photoelectric sensor is a photodiode.

6. The handheld card strip detection device according to claim 1, characterized in that: The outer surface of the upper half (2) of the housing is provided with a slidable sliding cover (7), and the optical module (16) is a replaceable structure.

7. The handheld card strip detection device according to any one of claims 1 to 6, characterized in that: The power supply current of the light source is controlled by the circuit part.

8. The handheld card strip detection device according to claim 4, characterized in that: The number of the metal slits (43) and the number of the emitting lenses (38) are both set to 1-3.

9. Application of the handheld card strip detection device described in any one of claims 1 to 8 in card strip detection.

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