Sampling head and sampling method of an online pollen bioaerosol detection device

Abstract

The application provides a sampling head and a sampling method of an online pollen bioaerosol detection device, and relates to the technical field of pollen bioaerosol sampling. The sampling head of the online pollen bioaerosol detection device comprises an outer cover, a separation cylinder, an upper cover plate and a sampling tube. The upper end surface of the separation cylinder is provided with a top-shaped special-shaped end surface. A funnel-shaped cyclone chamber is arranged at the cylinder body of the separation cylinder. The upper cover plate is coaxially crimped on the separation cylinder. The shaft center of the upper cover plate is provided with an exhaust pipe. The lower end surface of the upper cover plate is provided with a bottom-shaped special-shaped end surface. The bottom-shaped special-shaped end surface and the top-shaped special-shaped end surface are provided with a rotational flow type gas flow channel. One end of the sampling tube is inserted into the exhaust pipe. The other end of the sampling tube is connected with a suction device. The rotational flow type gas flow channel can collect pollen bioaerosols in the air from the circumferential direction of the sampling head, ensures the collection efficiency and avoids the influence of the sampling head by the wind direction.

Description

TECHNICAL FIELD

[0001] The application relates to the technical field of pollen bio-aerosol sampling, in particular to a sampling head of an online pollen bio-aerosol detection device and a sampling method. BACKGROUND

[0002] With the continuous change of global climate change and ecological environment, pollen bio-aerosol allergy has become an increasingly serious health problem, therefore, accurate and efficient monitoring and sampling of pollen bio-aerosol are of great significance for studying the causes of pollen bio-aerosol allergy, predicting pollen bio-aerosol seasons and formulating effective prevention measures.

[0003] Pollen bio-aerosol is different from other aerosols, first, the pollen bio-aerosol particle size is in the range of 5-100 um, most of which is in the range of 20-50 um, which is larger than that of other aerosols, and the suspension time in the air is shorter; second, pollen bio-aerosol is a biological body, and the surface thereof is often provided with sticky substances such as sugars, proteins and vegetable fats, which are easy to adhere to the sampling device, thereby affecting the sampling efficiency; third, because of the weight and stickiness of pollen bio-aerosol, the sampling direction and the wind direction have a great influence on the sampling efficiency; in addition, some dust and other large particle substances (100 um or more) in the air are easy to contaminate the sampling device and affect the sampling effect.

[0004] Therefore, there is an urgent need for an online pollen bio-aerosol detection device sampling head which is not affected by the wind direction, directly samples the air, filters out particles of 100 um or more, and has small wall loss. SUMMARY

[0005] The application aims to at least solve one of the technical problems existing in the prior art. To this end, the application provides a sampling head of an online pollen bio-aerosol detection device, which comprises an outer cover, a separation cylinder, an upper cover plate and a sampling tube, the separation cylinder is sealingly embedded in the outer cover, the upper end face of the separation cylinder is provided with a top-shaped special-shaped end face, and a funnel-shaped cyclone chamber is arranged at the cylinder body of the separation cylinder; the upper cover plate is coaxially crimped on the separation cylinder, the shaft center of the upper cover plate is provided with an exhaust pipe, the exhaust pipe is communicated with the funnel-shaped cyclone chamber, the lower end face of the upper cover plate is provided with a bottom-shaped special-shaped end face, and a rotational flow gas channel is arranged between the bottom-shaped special-shaped end face and the top-shaped special-shaped end face, the rotational flow gas channel is communicated with the funnel-shaped cyclone chamber; one end of the sampling tube is inserted into the exhaust pipe, and the other end of the sampling tube is connected with a suction device.

[0006] Preferably, the bottom end of the outer cover is coaxially detachably connected with a fixing plate, and the top end of the outer cover is coaxially detachably connected with a rain cover.

[0007] Preferably, the top side special-shaped end face comprises a bottom end plane part one, an outer side inclined plane part one, a top end plane part one and an inner side inclined plane part one, the outer side inclined plane part one is arranged at the inner side of the bottom end plane part one, the inner side of the outer side inclined plane part one is higher than the outer side; the top end plane part one is arranged at the inner side of the outer side inclined plane part one; the inner side inclined plane part one is arranged at the inner side of the top end plane part one, and the inner side of the inner side inclined plane part one is lower than the outer side.

[0008] Preferably, a plurality of louver structures are arranged on the outer side inclined plane part one in a circumferential direction, and the plurality of louver structures are arranged in a radial direction of the outer side inclined plane part one.

[0009] Preferably, a plurality of cyclone structures are arranged on the inner side inclined plane part one in a circumferential direction, and the plurality of cyclone structures are arranged in a radial direction of the inner side inclined plane part one, and the inclination direction of the cyclone structure is consistent with the inclination direction of the louver structure.

[0010] Preferably, the bottom side special-shaped end face comprises a bottom end plane part two, an outer side inclined plane part two, a top end plane part two and an inner side inclined plane part two, the bottom end plane part two corresponds to the bottom end plane part one; the outer side inclined plane part two is arranged at the inner side of the bottom end plane part two, the inner side of the outer side inclined plane part two is higher than the outer side, and the outer side inclined plane part two corresponds to the outer side inclined plane part one; the top end plane part two is arranged at the inner side of the outer side inclined plane part two, and the top end plane part two corresponds to the top end plane part one; the inner side inclined plane part two is arranged at the inner side of the top end plane part two, the inner side of the inner side inclined plane part two is lower than the outer side, and the inner side inclined plane part two corresponds to the inner side inclined plane part one.

[0011] Preferably, the upper and lower ends of the louver structure are connected to the outer side inclined plane part one and the outer side inclined plane part two respectively, and the plurality of louver structures between the separation cylinder and the upper cover plate form a first spiral air channel.

[0012] Preferably, the upper and lower ends of the cyclone structure are connected to the inner side inclined plane part one and the inner side inclined plane part two respectively, and the plurality of cyclone structures between the separation cylinder and the upper cover plate form a second spiral air channel.

[0013] Preferably, the cyclone type gas flow channel between the bottom side special-shaped end face and the top side special-shaped end face is composed of the first spiral air channel and the second spiral air channel.

[0014] The sampling head of the online pollen bioaerosol detection device has the following beneficial effects:

[0015] 1. The pollen bioaerosol in the air can be collected from the circumferential direction of the sampling head by using the cyclone type gas flow channel, so as to ensure the collection efficiency and avoid the influence of the sampling head by the wind direction;

[0016] 2. The use of swirling gas channels can make the airflow form a uniform annular laminar airflow, which can improve the swirling effect in the funnel-shaped swirling chamber and improve the separation effect of large and small particles in the airflow.

[0017] 3. The use of a swirling gas flow channel can further ensure the balance of suction around the sampling head.

[0018] On the other hand, this application also provides a sampling method for an online pollen bioaerosol detection device, comprising the following steps:

[0019] S1: Pump suction, which provides suction to the sampling tube through an external suction device, forcing the sampling tube to provide suction into the separation cylinder, thereby making the flow channel between the separation cylinder and the upper cover plate uniformly adsorb the air containing pollen bioaerosols in the circumferential direction.

[0020] S2, initial swirling flow: outside air is adsorbed around the separator and the top cover plate and enters between the top and bottom irregular end faces. Under the influence of the swirling gas flow channel, the airflow forms a swirling flow.

[0021] S3, Separation: The swirling airflow enters the funnel-shaped cyclone chamber. As the airflow channel gradually narrows, the gas spirals downward within the funnel-shaped cyclone chamber. During this process, large particles of impurities in the airflow fall tangentially downward along the outer wall of the funnel-shaped cyclone chamber, while small aerosol particles rise along the central normal region of the funnel-shaped cyclone chamber under the pressure of the bottom cyclone and are eventually pumped by the sampling tube and sent to the detection equipment.

[0022] Additional aspects and advantages of this application will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of this application. Attached Figure Description

[0023] To more clearly illustrate the technical solutions of the embodiments of this application, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this application and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained from these drawings without creative effort.

[0024] Figure 1 This is a schematic diagram of the overall structure of the sampling head of an online pollen bioaerosol detection device according to an embodiment of this application;

[0025] Figure 2 This is a cross-sectional view of the sampling head of an online pollen bioaerosol detection device according to an embodiment of this application;

[0026] Figure 3This is an exploded view of the sampling head of an online pollen bioaerosol detection device according to an embodiment of this application;

[0027] Figure 4 This is an exploded view of a partial structure between the separation cylinder and the upper cover plate according to an embodiment of this application;

[0028] Figure 5 This is a schematic diagram of the structure between the separation cylinder and the upper cover plate according to an embodiment of this application;

[0029] Figure 6 This is a cross-sectional view of the separation cylinder and the upper cover plate according to an embodiment of this application;

[0030] Figure 7 According to the embodiments of this application Figure 6 A magnified view of A in the middle.

[0031] Icons: 1. Outer cover; 11. Fixing plate; 12. Rain cover; 2. Separation cylinder; 21. Top side irregular end face; 211. Bottom flat part one; 212. Outer beveled part one; 213. Top flat part one; 214. Inner beveled part one; 22. Funnel-shaped cyclone chamber; 23. Auction structure; 24. Swirl structure; 3. Top cover plate; 31. Exhaust pipe; 32. Bottom side irregular end face; 321. Bottom flat part two; 322. Outer beveled part two; 323. Top flat part two; 324. Inner beveled part two; 33. Telescopic component; 331. Telescopic tube; 332. Spring; 34. Adjusting component; 341. End plate; 342. Guide rod; 343. Screw; 4. Sampling tube; 5. Ash hopper; 6. Dustproof net. Detailed Implementation

[0032] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this application, not all of them. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0033] Example 1, as Figures 1-5 As shown, the sampling head of an online pollen bioaerosol detection device according to an embodiment of this application includes an outer cover 1, a separation cylinder 2, an upper cover plate 3, and a sampling tube 4. The separation cylinder 2 is sealed and embedded in the outer cover 1. Specifically, it can be detachable by means of threaded connection, which will not be described in detail here.

[0034] like Figure 1As shown, a fixing plate 11 is detachably and coaxially connected to the bottom end of the outer cover 1, and a rain cover 12 is detachably and coaxially connected to the top end of the outer cover 1. The fixing plate 11 is sealed and fixed to the pollen bioaerosol detection equipment by a sealing ring and bolts.

[0035] like Figures 2-5 As shown, the upper end face of the separator 2 is provided with a top side irregular end face 21, and the body of the separator 2 is provided with a funnel-shaped cyclone chamber 22.

[0036] It should be noted that, as Figure 4 As shown, the top-side irregular end face 21 includes a bottom flat portion 211, an outer beveled portion 212, a top flat portion 213, and an inner beveled portion 214. The outer beveled portion 212 is disposed inside the bottom flat portion 211, and the inner side of the outer beveled portion 212 is higher than the outer side. The top flat portion 213 is disposed inside the outer beveled portion 212. The inner beveled portion 214 is disposed inside the top flat portion 213, and the inner side of the inner beveled portion 214 is lower than the outer side.

[0037] Furthermore, the upper cover plate 3 is coaxially pressed onto the separation cylinder 2. An exhaust pipe 31 is provided at the axial center of the upper cover plate 3. The exhaust pipe 31 is connected to the funnel-shaped cyclone chamber 22. The lower end face of the upper cover plate 3 is provided with a bottom side irregular end face 32. A swirling gas flow channel is provided between the bottom side irregular end face 32 and the top side irregular end face 21. The swirling gas flow channel is connected to the funnel-shaped cyclone chamber 22.

[0038] It should be noted that the bottom irregular end face 32 includes a bottom flat portion 321, an outer beveled portion 322, a top flat portion 323, and an inner beveled portion 324. The bottom flat portion 321 corresponds to the bottom flat portion 211. The outer beveled portion 322 is located inside the bottom flat portion 321, and the inner side of the outer beveled portion 322 is higher than the outer side. The outer beveled portion 322 corresponds to the outer beveled portion 212. The top flat portion 323 is located inside the outer beveled portion 322, and the top flat portion 323 corresponds to the top flat portion 213. The inner beveled portion 324 is located inside the top flat portion 323, and the inner side of the inner beveled portion 324 is lower than the outer side. The inner beveled portion 324 corresponds to the inner beveled portion 214.

[0039] Furthermore, such as Figures 3-5 As shown, multiple ballast structures 23 are uniformly arranged circumferentially on the outer inclined surface 212. The multiple ballast structures 23 are arranged radially inclined along the outer inclined surface 212. Multiple vortex structures 24 are uniformly arranged circumferentially on the inner inclined surface 214. The multiple vortex structures 24 are arranged radially inclined along the inner inclined surface 214, and the tilting direction of the vortex structures 24 is consistent with the tilting direction of the ballast structures 23.

[0040] The upper and lower ends of the labyrinth structure 23 are connected to the outer side slope part one 212 and the outer side slope part two 322, respectively, and the plurality of labyrinth structures 23 between the separation cylinder 2 and the upper cover plate 3 form a first spiral air passage. The upper and lower ends of the cyclone structure 24 are connected to the inner side slope part one 214 and the inner side slope part two 324, respectively, and the plurality of cyclone structures 24 between the separation cylinder 2 and the upper cover plate 3 form a second spiral air passage.

[0041] It can be understood that the cyclone type gas flow passage between the bottom side special-shaped end face 32 and the top side special-shaped end face 21 is composed of the first spiral air passage and the second spiral air passage.

[0042] One end of the sampling pipe 4 is inserted into the exhaust pipe 31, and the other end of the sampling pipe 4 is connected to the suction device after sequentially penetrating the upper cover plate 3, the separation cylinder 2 and the fixed plate 11. It can be understood that the suction device can adopt a vacuum pump or a motor, as long as a certain degree of negative pressure can be formed in the sampling pipe 4.

[0043] As shown in FIGS. 1, 2 and 3, the sampling head of the online pollen bioaerosol detection device according to the embodiment of the present application comprises a separation cylinder 2, an upper cover plate 3, a fixed plate 11, a funnel-shaped cyclone chamber 22, a dustproof net 6, a dust hopper 5, a sampling pipe 4 and a suction device. Figure 1 and Figure 2 As shown in FIGS. 1, 2 and 3, the sampling head of the online pollen bioaerosol detection device according to the embodiment of the present application comprises a separation cylinder 2, an upper cover plate 3, a fixed plate 11, a funnel-shaped cyclone chamber 22, a dustproof net 6, a dust hopper 5, a sampling pipe 4 and a suction device.

[0044] The use process of the sampling head of the online pollen bioaerosol detection device according to the embodiment of the present application is described below with reference to the accompanying drawings:

[0045] In use, the suction tube 4 is connected to an external suction device, and the suction tube 4 is connected to the exhaust pipe 31 of the upper cover plate 3. The suction device is started to form a suction in the suction tube 4, and then the cyclone gas flow channel between the separation cylinder 2 and the upper cover plate 3 is forced to produce a suction to the outside. The plurality of cyclone structures 24 and the plurality of labyrinth structures 23 cooperate with the top-shaped end surface 21 and the bottom-shaped end surface 32 to form a second spiral gas channel on the inside and a first spiral gas channel on the outside, respectively. The cyclone structure 24 and the labyrinth structure 23 are inclined in the same direction, so that the outside air is sucked into the first spiral gas channel on the outside and forms a rotating state to a certain extent. After the rotating gas flow enters the second spiral gas channel on the inside, it converges and further rotates into the funnel-shaped cyclone chamber 22. As the diameter of the funnel-shaped cyclone chamber 22 gradually decreases, the rotating gas flow accelerates due to the decrease in the radius of the annular surface, and the pressure of the gas flow increases. The fluid is pressed to move upward along the central normal line of the funnel-shaped cyclone chamber 22. Due to the centrifugal force, the large particle impurities in the gas flow run along the wall surface of the funnel-shaped cyclone chamber 22 and fall downward into the ash hopper 5. The small particle aerosols in the gas flow are sampled by the suction tube 4. In this process, it should be noted that the cyclone gas flow channel formed by the first spiral gas channel and the second spiral gas channel is arranged in a high-low manner, so that water droplets or large particle impurities can be prevented from entering the sampling head to a certain extent. At the same time, the spiral shape of the first spiral gas channel and the second spiral gas channel and the high-low arrangement can also prevent the influence of the atmospheric wind direction and the turbulence formed during the adsorption of the gas flow on the sampling to a certain extent. The design of the device can directly sample pollen biological aerosols in the air without mixing the solution and filtering the sample.

[0046] In the second embodiment, the power source of the entire sampling head is the suction provided by the suction tube 4 at the exhaust pipe 31 of the upper cover plate 3. Therefore, in the case where the suction is constant, the depth of the exhaust pipe 31 in the funnel-shaped cyclone chamber 22 will interfere with the effective formation of the cyclone or the separation effect of the cyclone.

[0047] According to some embodiments of the present application, as shown in Figure 6 and Figure 7 , the elastic expansion piece 33 is elastically inserted into the exhaust pipe 31. The elastic expansion piece 33 includes an expansion tube 331 which is limited to slide at the bottom of the exhaust pipe 31. A spring 332 is arranged between the expansion tube 331 and the exhaust pipe 31. Specifically, one end of the spring 332 abuts against the top of the expansion tube 331, and the other end of the spring 332 abuts against the exhaust pipe 31, so that the expansion tube 331 has an elastic expansion function in the exhaust pipe 31.

[0048] Further, the upper end of the telescopic tube 331 is also connected with an adjusting member 34, the adjusting member 34 comprises an end plate 341 sleeved on the sampling tube 4, a plurality of guide rods 342 are circumferentially fixed on the end plate 341, and a screw rod 343 is limitingly inserted on the end plate 341 (axial limiting, that is, the two cannot have relative axial displacement), wherein the plurality of guide rods 342 are slidingly inserted on the exhaust pipe 31 and fixed on the telescopic tube 331, the screw rod 343 is threadedly connected with the exhaust pipe 31, and one end of the screw rod 343 away from the end plate 341 abuts against the telescopic tube 331.

[0049] Thus, after the sampling tube 4 is inserted into the exhaust pipe 31, the screw rod 343 can be rotated to drive the end plate 341 to have axial displacement along the exhaust pipe 31, and the downward axial displacement is taken as an example for illustration, as shown in Figure 7 When the screw rod 343 drives the end plate 341 to have downward displacement, the telescopic tube 331 will be synchronously pushed downward by the screw rod 343 and the plurality of guide rods 342, at this time, the telescopic tube 331 gradually extends out of the bottom end of the exhaust pipe 31 and compresses the spring 332, conversely, reverse rotation of the screw rod 343 will drive the end plate 341 and the plurality of guide rods 342 to have upward displacement, at this time, the spring 332 will force the telescopic tube 331 to gradually retract toward the bottom end of the exhaust pipe 31 under the action of the elastic force, thus, the depth of the exhaust pipe 31 actually located in the funnel-shaped cyclone chamber 22 can be controlled in a fine adjustment mode, so that the suction force of the bottom end of the exhaust pipe 31 can not disturb the state of the cyclone in the funnel-shaped cyclone chamber 22 as much as possible.

[0050] On the other hand, the embodiment of the present application further provides a sampling method for an online pollen bioaerosol detection device, comprising the following steps:

[0051] S1: pumping, providing suction force to the sampling tube 4 by an external suction device, forcing the sampling tube 4 to provide suction force to the separation cylinder 2, and then making the flow channel between the separation cylinder 2 and the upper cover plate 3 uniformly adsorb the air containing pollen bioaerosol outside in the circumferential direction;

[0052] S2, preliminary cyclone, the air outside is adsorbed into the space between the top-shaped end face 21 and the bottom-shaped end face 32 on the circumferential side of the separation cylinder 2 and the upper cover plate 3, and is affected by the cyclone gas flow channel guide to form a cyclone;

[0053] S3, separation, the airflow forming a cyclone enters the funnel-shaped cyclone chamber 22, is affected by the gradually reduced airflow channel, and forms a spiral downward in the funnel-shaped cyclone chamber 22, and in this process, large-particle impurities in the airflow drop along the peripheral wall of the funnel-shaped cyclone chamber 22, and small-particle aerosols in the airflow rise along the central normal region of the funnel-shaped cyclone chamber 22 under the action of the bottom cyclone pressure, and are finally pumped by the sampling tube 4 and sent to the detection device.

[0054] It should be noted that the specific model and specifications of the rain cover 12, the spring 332, the screw rod 343, the sampling pipe 4, the ash bucket 5 and the dust screen 6 need to be selected and determined according to the actual specifications of the device, and the specific selection calculation method adopts the existing technology in the art, so it is not described in detail.

[0055] The preferred embodiments of the present application have been described above, and are not intended to limit the present application. Those skilled in the art can make various modifications and changes to the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A sampling head for an online pollen bioaerosol detection apparatus, characterized in that, The utility model relates to a kind of sampling head of online pollen bioaerosol detection equipment, comprising the following steps: Housing; Separation cylinder, the separation cylinder is sealedly embedded in the housing, the upper end surface of the separation cylinder is provided with top side special-shaped end face, funnel-shaped cyclone chamber is provided at the barrel of the separation cylinder; Upper cover plate, the upper cover plate is coaxially pressure bonded in the separation cylinder, the axis of the upper cover plate is provided with exhaust pipe, the exhaust pipe and the funnel-shaped cyclone chamber are communicated, the lower end surface of the upper cover plate is provided with bottom side special-shaped end face, bottom side special-shaped end face and the top side special-shaped end face are provided with rotational flow gas flow channel, and the rotational flow gas flow channel and the funnel-shaped cyclone chamber are communicated; Sampling tube, one end of the sampling tube is inserted into the exhaust pipe, the other end of the sampling tube is connected with suction device; The top side special-shaped end face includes: Bottom end plane part one; Outer side inclined surface part one, the outer side inclined surface part one is arranged on the inner side of the bottom end plane part one, and the inner side of the outer side inclined surface part one is higher than the outer side; Top end plane part one, the top end plane part one is arranged on the inner side of the outer side inclined surface part one; Inner side inclined surface part one, the inner side inclined surface part one is arranged on the inner side of the top end plane part one, and the inner side of the inner side inclined surface part one is lower than the outer side; A plurality of diaphragm structures are uniformly arranged on the outer side inclined surface part one in the circumferential direction, and the plurality of diaphragm structures are arranged in the radial direction of the outer side inclined surface part one in an inclined manner; A plurality of rotational flow structures are uniformly arranged on the inner side inclined surface part one in the circumferential direction, and the plurality of rotational flow structures are arranged in the radial direction of the inner side inclined surface part one in an inclined manner, and the inclined direction of the rotational flow structure is consistent with the inclined direction of the diaphragm structure; The bottom side special-shaped end face includes: Bottom end plane part two, the bottom end plane part two corresponds to the bottom end plane part one; Outer side inclined surface part two, the outer side inclined surface part two is located on the inner side of the bottom end plane part two, the inner side of the outer side inclined surface part two is higher than the outer side, and the outer side inclined surface part two corresponds to the outer side inclined surface part one; Top end plane part two, the top end plane part two is located on the inner side of the outer side inclined surface part two, and the top end plane part two corresponds to the top end plane part one; Inner side inclined surface part two, the inner side inclined surface part two is located on the inner side of the top end plane part two, the inner side of the inner side inclined surface part two is lower than the outer side, and the inner side inclined surface part two corresponds to the inner side inclined surface part one; The upper end and the lower end of the diaphragm structure are connected to the outer side inclined surface part one and the outer side inclined surface part two respectively, and the plurality of diaphragm structures between the separation cylinder and the upper cover plate form a first spiral gas channel; The upper end and the lower end of the rotational flow structure are connected to the inner side inclined surface part one and the inner side inclined surface part two respectively, and the plurality of rotational flow structures between the separation cylinder and the upper cover plate form a second spiral gas channel; The rotational flow gas flow channel between the bottom side special-shaped end face and the top side special-shaped end face is composed of the first spiral gas channel and the second spiral gas channel.

2. The sampling head of an online pollen bioaerosol detection apparatus according to claim 1, wherein, The bottom end of the housing is coaxially detachably connected with a fixing plate, and the top end of the housing is coaxially detachably connected with a rain cover.

3. A method of sampling for an online pollen bioaerosol detection apparatus, characterized in that, The sampling head of the online pollen bioaerosol detection equipment according to any one of claims 1-2 comprises the following steps: S1, pumping, the sampling tube is provided with suction force by the external suction device, which forces the sampling tube to provide suction force to the separation cylinder, and then the flow channel between the separation cylinder and the upper cover plate uniformly absorbs the air containing pollen bioaerosol from the outside; S2, preliminary cyclone, the outside air is absorbed into the top and bottom special-shaped end faces between the separation cylinder and the upper cover plate, and is affected by the cyclone gas flow guide, so that the airflow forms a cyclone; S3, separation, the airflow forming a cyclone enters the funnel-shaped cyclone chamber, and is affected by the gradually narrowing airflow channel, the gas forms a spiral downward in the funnel-shaped cyclone chamber, and in this process, the large particle impurities in the airflow fall downward along the peripheral wall of the funnel-shaped cyclone chamber, and the small particle aerosol in the airflow rises along the central normal area of the funnel-shaped cyclone chamber under the action of the bottom cyclone pressure, and is finally pumped by the sampling tube to the detection equipment.

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