Green sterilization method for vibrio parahaemolyticus in aquatic products

Abstract

The invention provides a green sterilization method for vibrio parahaemolyticus in aquatic products, and relates to the field of sterilization. The green sterilization method for vibrio parahaemolyticus in the aquatic product comprises the following steps: placing the aquatic product on a clean operation table, uniformly spraying subacid electrolyzed water on the surface of the aquatic product by using a spraying device, ensuring that the spraying amount can cover the whole surface of the aquatic product, generally 30mL / m < 2 >, and enabling the electrolyzed water to stay on the surface of the aquatic product for 5 minutes, the depth of the electrolyzed water in the tank is enough to completely immerse the aquatic products to be treated, the polluted aquatic products are completely immersed in the electrolyzed water, enough gaps are ensured between the aquatic products, and the aquatic products are immersed for 10 minutes. The vibrio parahaemolyticus is sterilized by using the subacid electrolyzed water, so that the environment is not polluted, harmful substances are not remained in the aquatic products, and the aquatic products are treated by using a green sterilization method, so that the damage to nutritional ingredients such as protein, fat and the like of the aquatic products is effectively avoided.

Description

Technical Field

[0001] The invention relates to the technical field of sterilization, in particular to a green sterilization method for Vibrio parahaemolyticus in aquatic products. Background Art

[0002] With the increasing global attention to food safety and environmental protection, the development and application of green sterilization technology has become an important research direction in the field of aquatic product processing. Vibrio parahaemolyticus, as a common foodborne pathogen, is widely present in marine and estuarine environments, especially in aquatic products, with a high detection rate. The foodborne diseases caused by this bacterium pose a serious threat to consumer health, so it is crucial to effectively control it during the processing and storage of aquatic products.

[0003] Although the prior art uses chemical bactericides to sterilize Vibrio parahaemolyticus in aquatic products, there is a problem that chemical bactericides may remain in aquatic products, thereby affecting product quality and safety. Long-term use of chemical bactericides may cause bacteria to develop drug resistance and reduce the bactericidal effect. Therefore, those skilled in the art provide a green sterilization method for Vibrio parahaemolyticus in aquatic products to solve the problems raised in the above background technology. Summary of the invention

[0004] 1. Technical issues to be solved

[0005] In view of the deficiencies in the prior art, the present invention provides a green sterilization method for Vibrio parahaemolyticus in aquatic products, which reduces the negative impact on the environment during the sterilization process and avoids the problem of residual effective substances in aquatic products.

[0006] (II) Technical solution

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions: A green sterilization method for Vibrio parahaemolyticus in aquatic products, comprising the following steps:

[0008] S1. Collect 25g or 25ml of sample from aquatic products, add buffer to the sample for homogenization, dilute the homogenized sample by 10 times, 100 times and 1000 times, and inoculate the samples with different dilutions on selective culture medium, TCBS agar medium is used here, and cultured at 37°C for 18 hours;

[0009] S2. Prepare food-grade sodium chloride and deionized water, and select a slightly acidic electrolyzed water generator, a liquid storage tank, a spraying device, and a soaking tank;

[0010] S3, add appropriate amount of deionized water and sodium chloride to the liquid storage tank and keep stirring, add the prepared electrolyte to the slightly acidic electrolyzed water generator, start the electrolyzed water generator and continue electrolysis for 40 minutes, use a pH meter and an effective chlorine concentration meter to monitor the pH value and effective chlorine concentration of the electrolyzed water, and ensure that the pH value of the slightly acidic electrolyzed water is between 5.0 and 5.6, and the effective chlorine concentration is between 40 and 80 mg / L;

[0011] S4. Place the aquatic product on a clean operating table and use a spraying device to evenly spray the slightly acidic electrolyzed water on the surface of the aquatic product, ensuring that the spraying volume can cover the entire surface of the aquatic product, usually 30mL / m 2 , let the electrolyzed water stay on the surface of the aquatic product for 5 minutes, and at the same time, inject the slightly acidic electrolyzed water into the immersion tank, making sure that the depth of the electrolyzed water in the tank is enough to completely immerse the aquatic product to be treated, and completely immerse the contaminated aquatic product in the electrolyzed water, making sure there is enough space between the aquatic products, and let the aquatic products soak for 10 minutes;

[0012] S5. After the treatment is completed, take out the aquatic product, rinse the surface of the aquatic product with clean water for 1 minute to remove the residual electrolyzed water, and use a clean cloth to wipe the moisture on the surface of the aquatic product. At this time, sample the aquatic product again according to the steps of S1 to detect the residual colonies, and package the remaining aquatic products in packaging that meets food safety standards and store them in a low temperature environment below 7.2°C. At the same time, the remaining slightly acidic electrolyzed water can be directly discharged into the sewer.

[0013] Preferably, the following formula is used in the bacterial colony culture process in S1:

[0014] Colony forming unit calculation: CFU / g or

[0015] Among them, CFU is colony forming unit;

[0016] Calculation of bacterial concentration: total bacterial count = CFU / g or CFU / mL × total dilution multiple.

[0017] Preferably, the weight ratio of food-grade sodium chloride in S2 is 0.1% to 0.2%.

[0018] Preferably, the following formula is used in S3:

[0019] Calculation of effective chlorine concentration:

[0020] Among them, C CI is the effective chlorine concentration, m CI is the mass of available chlorine, V SAEW is the volume of slightly acidic electrolyzed water;

[0021] Electrolysis efficiency calculation:

[0022] Among them, m actual is the actual mass of chlorine generated, m theoretical is the theoretical mass of chlorine generated.

[0023] Preferably, the following formula is used in S5:

[0024]

[0025] D value calculation:

[0026] Where D is the time required to kill microorganisms, t is the treatment time, N0 is the initial colony count, N t Colony count after treatment.

[0027] Preferably, in steps S1 to S5, the operator should wear protective gloves, goggles and protective clothing, and the electrolyzed water generator should be placed in a well-ventilated place to avoid inhaling chlorine generated during the electrolysis process.

[0028] Preferably, the slightly acidic electrolyzed water in S3 is prepared and used immediately, and the pH meter and the effective chlorine concentration meter are calibrated regularly, and the electrolyzed water generator is cleaned and maintained regularly.

[0029] (III) Beneficial effects

[0030] The present invention provides a green sterilization method for Vibrio parahaemolyticus in aquatic products, which has the following beneficial effects:

[0031] 1. Vibrio parahaemolyticus is sterilized by using slightly acidic electrolyzed water. Since slightly acidic electrolyzed water has a significant killing effect on foodborne pathogens, and slightly acidic electrolyzed water decomposes into water and oxygen after use, it will not pollute the environment, nor will it leave harmful substances in aquatic products. At the same time, slightly acidic electrolyzed water is harmless to the human body and safe to use. In addition, the equipment for generating the substance is simple and easy to operate, making it suitable for large-scale application.

[0032] 2. By using green sterilization methods to treat aquatic products, the pH value of slightly acidic electrolyzed water is between 5.0-6.5, which is close to the natural pH value of aquatic products. It can effectively avoid the destruction of nutrients such as protein and fat in aquatic products, and has little effect on the texture and flavor of aquatic products.

[0033] 3. When the slightly acidic electrolyzed water is treated after use, the slightly acidic electrolyzed water is mainly generated by electrolysis of water and a small amount of sodium chloride, without adding other chemical reagents. The treated wastewater does not contain persistent organic pollutants and is environmentally friendly. The wastewater treated with slightly acidic electrolyzed water can be discharged without special treatment, reducing the cost of sewage treatment. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 It is a schematic diagram of the process of the present invention;

[0035] Figure 2 A schematic diagram of the process of sampling the bacterial flora of the present invention;

[0036] Figure 3 Schematic diagram of the process of slightly acidic electrolyzed water of the present invention;

[0037] Figure 4 It is a schematic diagram of the subsequent processing flow of the present invention. DETAILED DESCRIPTION

[0038] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0039] Embodiment 1:

[0040] like Figure 1-4 As shown, an embodiment of the present invention provides a green sterilization method for Vibrio parahaemolyticus in aquatic products, comprising the following steps:

[0041] S1. Collect 25 grams or 25 milliliters of samples from aquatic products, add buffer to the samples for homogenization, dilute the homogenized samples by 10 times, 100 times and 1000 times, and inoculate the samples with different dilutions onto selective culture medium, TCBS agar medium is used here, and cultured at 37°C for 18 hours.

[0042] S2. Prepare food-grade sodium chloride and deionized water, and select a slightly acidic electrolyzed water generator, a liquid storage tank, a spraying device, and a soaking tank.

[0043] S3. Add appropriate amount of deionized water and sodium chloride to the liquid storage tank and stir continuously, add the prepared electrolyte to the slightly acidic electrolyzed water generator, start the electrolyzed water generator and continue electrolysis for 40 minutes, use a pH meter and an effective chlorine concentration meter to monitor the pH value and effective chlorine concentration of the electrolyzed water, ensure that the pH value of the slightly acidic electrolyzed water is between 5.0 and 5.6, and the effective chlorine concentration is between 40 and 80 mg / L, and use slightly acidic electrolyzed water to sterilize Vibrio parahaemolyticus. Since slightly acidic electrolyzed water has a significant killing effect on foodborne pathogens, and slightly acidic electrolyzed water is decomposed into water and oxygen after use, it will not cause pollution to the environment, and no harmful substances will remain in aquatic products. At the same time, slightly acidic electrolyzed water is harmless to the human body and safe to use. In addition, the material generation equipment is simple and easy to operate, which is suitable for large-scale application.

[0044] S4. Place the aquatic product on a clean operating table and use a spraying device to evenly spray the slightly acidic electrolyzed water on the surface of the aquatic product, ensuring that the spraying volume can cover the entire surface of the aquatic product, usually 30mL / m 2 , let the electrolyzed water stay on the surface of the aquatic product for 5 minutes, and at the same time inject the slightly acidic electrolyzed water into the immersion tank, make sure the depth of the electrolyzed water in the tank is sufficient to completely immerse the aquatic products to be treated, immerse the contaminated aquatic products completely in the electrolyzed water, make sure there is enough space between the aquatic products, and let the aquatic products soak for 10 minutes. By using the green sterilization method to treat the aquatic products, the pH value of the slightly acidic electrolyzed water is between 5.0-6.5, which is close to the natural pH value of aquatic products. It can effectively avoid damage to the protein, fat and other nutrients of the aquatic products, and has little effect on the texture and flavor of the aquatic products.

[0045] S5. After the treatment is completed, the aquatic product is taken out, and the surface of the aquatic product is rinsed with clean water for 1 minute to remove the residual electrolyzed water, and the moisture on the surface of the aquatic product is wiped with a clean cloth. At this time, the aquatic product is sampled again according to the steps of S1 to detect the residual colonies, and the remaining aquatic products are packaged in packaging that meets the food safety standards and stored in a low temperature environment below 7.2°C. At the same time, the remaining slightly acidic electrolyzed water can be directly discharged into the sewer. When the slightly acidic electrolyzed water is used for treatment, the slightly acidic electrolyzed water is mainly generated by electrolysis of water and a small amount of sodium chloride without adding other chemical reagents. The treated wastewater does not contain persistent organic pollutants and is environmentally friendly. The wastewater treated with slightly acidic electrolyzed water can be discharged without special treatment, thereby reducing the cost of sewage treatment.

[0046] The following formula is used during the colony culture process in S1:

[0047] Colony forming unit calculation: CFU / g or

[0048] Among them, CFU is colony forming unit;

[0049] Calculation of bacterial concentration: total bacterial count = CFU / g or CFU / mL × total dilution multiple.

[0050] The weight ratio of food grade sodium chloride in S2 is 0.1% to 0.2%.

[0051] The following formula is used in S3:

[0052] Calculation of effective chlorine concentration:

[0053] Among them, C CI is the effective chlorine concentration, m CI is the mass of available chlorine, V SAEW is the volume of slightly acidic electrolyzed water;

[0054] Electrolysis efficiency calculation:

[0055] Among them, m actual is the actual mass of chlorine generated, m theoretical is the theoretical mass of chlorine generated.

[0056] The following formula is used in S5:

[0057]

[0058] D value calculation:

[0059] Where D is the time required to kill microorganisms, t is the treatment time, N0 is the initial colony count, N t Colony count after treatment.

[0060] In steps S1 to S5, the operator should wear protective gloves, goggles and protective clothing, and the electrolyzed water generator should be placed in a well-ventilated place to avoid inhaling chlorine generated during the electrolysis process. According to a green sterilization method for Vibrio parahaemolyticus in aquatic products according to claim 1, it is characterized in that: the slightly acidic electrolyzed water in S3 is prepared and used immediately, and the pH meter and the effective chlorine concentration meter are calibrated regularly, and the electrolyzed water generator is cleaned and maintained regularly.

[0061] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A green sterilization method for Vibrio parahaemolyticus in aquatic products, characterized by: The following steps are involved: S1. Collect 25g or 25ml of sample from aquatic products, add buffer to the sample for homogenization, dilute the homogenized sample by 10 times, 100 times and 1000 times, and inoculate the samples with different dilutions on selective culture medium, TCBS agar medium is used here, and cultured at 37°C for 18 hours; S2. Prepare food-grade sodium chloride and deionized water, and select a slightly acidic electrolyzed water generator, a liquid storage tank, a spraying device, and a soaking tank; S3, add appropriate amount of deionized water and sodium chloride to the liquid storage tank and keep stirring, add the prepared electrolyte to the slightly acidic electrolyzed water generator, start the electrolyzed water generator and continue electrolysis for 40 minutes, use a pH meter and an effective chlorine concentration meter to monitor the pH value and effective chlorine concentration of the electrolyzed water, and ensure that the pH value of the slightly acidic electrolyzed water is between 5.0 and 5.6, and the effective chlorine concentration is between 40 and 80 mg / L; S4. Place the aquatic product on a clean operating table and use a spraying device to evenly spray the slightly acidic electrolyzed water on the surface of the aquatic product, ensuring that the spraying volume can cover the entire surface of the aquatic product, usually 30mL / m 2 , let the electrolyzed water stay on the surface of the aquatic product for 5 minutes, and at the same time, inject the slightly acidic electrolyzed water into the immersion tank, making sure that the depth of the electrolyzed water in the tank is enough to completely immerse the aquatic product to be treated, and completely immerse the contaminated aquatic product in the electrolyzed water, making sure there is enough space between the aquatic products, and let the aquatic products soak for 10 minutes; S5. After the treatment is completed, take out the aquatic product, rinse the surface of the aquatic product with clean water for 1 minute to remove the residual electrolyzed water, and use a clean cloth to wipe the moisture on the surface of the aquatic product. At this time, sample the aquatic product again according to the steps of S1 to detect the residual colonies, and package the remaining aquatic products in packaging that meets food safety standards and store them in a low temperature environment below 7.2°C. At the same time, the remaining slightly acidic electrolyzed water can be directly discharged into the sewer.

2. The green sterilization method for Vibrio parahaemolyticus in aquatic products according to claim 1, characterized in that: The following formula is used in the colony culture process in S1: Colony forming unit calculation: CFU / g or Among them, CFU is colony forming unit; Calculation of bacterial concentration: total bacterial count = CFU / g or CFU / mL × total dilution multiple.

3. The green sterilization method for Vibrio parahaemolyticus in aquatic products according to claim 1, characterized in that: The weight ratio of food-grade sodium chloride in S2 is 0.1% to 0.2%.

4. The green sterilization method for Vibrio parahaemolyticus in aquatic products according to claim 1, characterized in that: The following formula is used in S3: Calculation of effective chlorine concentration: Among them, C CI is the effective chlorine concentration, m CI is the mass of available chlorine, V SAEW is the volume of slightly acidic electrolyzed water; Electrolysis efficiency calculation: Among them, m actual is the actual mass of chlorine generated, m theoretical is the theoretical mass of chlorine generated.

5. The green sterilization method for Vibrio parahaemolyticus in aquatic products according to claim 1, characterized in that: The following formula is used in S5: D value calculation: Where D is the time required to kill microorganisms, t is the treatment time, N0 is the initial colony count, N t Colony count after treatment.

6. The green sterilization method for Vibrio parahaemolyticus in aquatic products according to claim 1, characterized in that: In the steps S1 to S5, the operator should wear protective gloves, goggles and protective clothing, and the electrolyzed water generator should be placed in a well-ventilated place to avoid inhaling chlorine gas generated during the electrolysis process.

7. The green sterilization method for Vibrio parahaemolyticus in aquatic products according to claim 1, characterized in that: The slightly acidic electrolyzed water in S3 is prepared and used immediately, and the pH meter and the effective chlorine concentration meter are calibrated regularly, and the electrolyzed water generator is cleaned and maintained regularly.

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