Ginseng extract composition, preparation method and application thereof

Abstract

The present invention discloses a ginseng extract composition and its preparation method and application, belonging to the field of extraction technology. The preparation method comprises extracting an enzyme-containing crude extract from fresh ginseng containing fresh stems and leaves, extracting taproot total saponins from the ginseng taproot, extracting fibrous root total saponins from the ginseng fibrous roots, dissolving and mixing the enzyme-containing crude extract, taproot total saponins, and fibrous root total saponins with a buffer solution, converting them at an enzyme-suitable temperature for a predetermined time to obtain a crude product system, and removing water and water-soluble impurities from the crude product system to obtain a ginseng extract composition. The present invention combines enzymes in fresh ginseng with saponins in the taproot and fibrous roots to promote the conversion and extraction of ginsenosides. The natural enzymes in fresh ginseng help convert saponins into components with higher biological activity. The obtained ginseng extract composition can not only treat alcoholic fatty liver disease, but also has a preventive effect on alcoholic fatty liver disease, and has a better effect than directly taking ginseng or taking a direct extract of the whole ginseng root.

Description

Technical Field

[0001] The invention relates to a ginseng extraction composition and a preparation method and application thereof, belonging to the field of extraction technology. Background Art

[0002] Excessive drinking, through ethanol itself and its derivative acetaldehyde, can cause repeated fatty degeneration, necrosis, and regeneration of liver cells, leading to alcoholic liver disease, such as alcoholic fatty liver. Starting with simple alcoholic fatty liver, if left untreated, the condition can gradually progress to alcoholic hepatitis, leading to liver fibrosis and, ultimately, the severe stage of alcoholic cirrhosis.

[0003] Common medications for treating alcoholic fatty liver disease include silybin capsules, liver-protecting capsules, compound Yiganling capsules, and Kaixilai tablets. However, these medications may cause adverse reactions such as dizziness and vomiting. In patients with allergies, these medications may also cause allergic reactions. Long-term, high-dose use may also cause liver dysfunction. Therefore, there is a need for a medication or food that can prevent and treat alcoholic fatty liver disease without toxic side effects, thereby preventing or alleviating the symptoms associated with alcoholic fatty liver disease. Summary of the Invention

[0004] In order to overcome the deficiencies of the prior art, the present invention provides a ginseng extract composition and a preparation method thereof, which can treat and prevent alcoholic fatty liver, and provides related applications.

[0005] The technical solution adopted by the present invention to solve its technical problem is:

[0006] In a first aspect, the present application provides a method for preparing a ginseng extract composition, comprising the following steps:

[0007] Extracting crude enzyme extract from fresh ginseng with fresh stems and leaves;

[0008] Extracting taproot total saponins from ginseng taproot;

[0009] Extracting total saponins from ginseng root fibers;

[0010] dissolving and mixing the crude enzyme extract, the main root total saponins, and the fibrous root total saponins with a buffer solution, and converting them at a temperature suitable for the enzyme for a predetermined time to obtain a crude production system;

[0011] The water and water-soluble impurities in the crude product system are removed to obtain the ginseng extract composition.

[0012] The preparation method of the ginseng extract composition provided in this application utilizes the enzyme activity in fresh ginseng to promote the conversion of saponins in the main root and fibrous root of ginseng. Experiments have confirmed that ginsenosides in specific parts are more conducive to the treatment and prevention of alcoholic fatty liver after conversion.

[0013] Furthermore, the step of dissolving and mixing the crude enzyme extract, the main root total saponins and the fibrous root total saponins with a buffer solution comprises:

[0014] Mixing the main root total saponins and the fibrous root total saponins to obtain a first mixture, and dissolving the first mixture with the buffer solution 10 times the mass of the first mixture to obtain a first solution;

[0015] dissolving the crude enzyme extract with the buffer solution that is 10 times the mass of the crude enzyme extract to obtain a second solution;

[0016] mixing equal masses of the first solution and the second solution;

[0017] The buffer solution is a phosphate buffer solution with a pH of 5.5 to 7.0.

[0018] This process can ensure that the enzyme activity is maintained and exerted, thereby more efficiently converting saponins, optimizing the effect of the enzymatic reaction, and ultimately improving the therapeutic effect of the ginseng extract composition.

[0019] Furthermore, in the first mixture, the mass ratio of the total saponins in the main root to the total saponins in the fibrous root is 2.6:1.

[0020] This specific mass ratio optimizes the ratio of saponins in the main root and fibrous roots, maximizing the effect of saponin conversion and contributing to the formation of richer active ingredients.

[0021] Furthermore, the step of extracting the enzyme-containing crude extract from fresh ginseng containing fresh stems and leaves comprises:

[0022] The fresh ginseng is crushed into a pulp at below 30° C., water is added and the mixture is centrifuged, the supernatants are combined, and the mixture is concentrated under reduced pressure at 30° C. to 40° C. to obtain a concentrate, the concentrate is mixed with 95% edible alcohol in an amount that is 3 times the volume of the concentrate, and the insoluble matter is collected by centrifugation to obtain the enzyme-containing crude extract.

[0023] This process protects the enzyme activity in fresh ginseng from being destroyed. Low-temperature treatment and reduced-pressure concentration effectively preserve the bioactive components in fresh ginseng, particularly the activity of enzymes. This process also results in a purer enzyme-containing crude extract, which helps improve the efficiency of subsequent saponin conversion and the final quality of the extract.

[0024] Furthermore, the main root total saponins and the fibrous root total saponins are extracted according to the following steps:

[0025] The roots of the relevant parts are crushed, and extracted with 8 to 10 times the amount of water by mass at 85° C. to 95° C. for at least 1 hour. The extraction is repeated multiple times, and the extracts are combined. The extracts are adsorbed with a macroporous adsorption resin, and the macroporous adsorption resin is rinsed with water to remove impurities. The macroporous adsorption resin is then eluted with 70% to 90% edible alcohol. Finally, the eluate is concentrated and dried to obtain saponins from the relevant parts.

[0026] This process can effectively extract saponin components from ginseng, which is beneficial to improving the purity and activity of the extract and providing purer basic materials for subsequent enzymatic conversion.

[0027] Furthermore, the requirement for the conversion at the enzyme suitable temperature for a preset time is: keeping the mixture at 40° C. for 48 h to 96 h under stirring at 80 rpm to 120 rpm.

[0028] In other words, the optimal temperature for the enzyme is 40°C, and the preset time is 48 to 96 hours. This process can fully utilize the catalytic effect of the enzyme in fresh ginseng, converting it into components with higher biological activity and achieving higher saponin conversion efficiency. The resulting extract is more effective in treating alcoholic fatty liver disease.

[0029] Furthermore, the step of removing water and water-soluble impurities in the crude product system comprises:

[0030] The crude product system was concentrated under reduced pressure to one tenth of its original volume to obtain a crude product concentrate, 3 times the volume of 95% edible alcohol as that of the crude product concentrate was added to the crude product concentrate, and then the supernatant was evaporated to dryness to obtain a crude product;

[0031] The crude product is dissolved in 40% to 50% edible alcohol and adsorbed on a macroporous adsorption resin. The macroporous adsorption resin is rinsed with water to remove impurities. The macroporous adsorption resin is then eluted with 85% to 95% edible alcohol. Finally, the eluate is concentrated and dried.

[0032] After purification and refinement, the purity of the extract can be greatly improved, which is beneficial to improving the quality of the extract and ensuring its higher safety and stability in subsequent use, thereby improving its effectiveness in the treatment and prevention of alcoholic fatty liver.

[0033] In a second aspect, the present application provides a ginseng extract composition, prepared by the ginseng extract composition preparation method described in the first aspect, which is particularly suitable for preventing and treating alcoholic fatty liver disease. Through optimized extraction and conversion processes, the resulting composition has higher activity.

[0034] Furthermore, the total ginsenoside content of ginsenosides is ≥90wt%, and contains ginsenosides S-Rg2, R-Rg2, S-Rg3, R-Rg3, Rg4, Rg5, Rg6, S-Rh1, R-Rh1, S-Rh2, R-Rh2, Rh3, Rh4, Rk1, Rk2, and Rk3.

[0035] In a third aspect, the present application provides an application of the ginseng extract composition as described in the second aspect, specifically mixed in a beverage or food.

[0036] The beneficial effects of the present invention are as follows: the present invention combines the enzymes in fresh ginseng containing fresh stems and leaves with the saponins in the main roots and fibrous roots, thereby promoting the conversion and extraction of ginsenosides. The natural enzymes in fresh ginseng help to convert saponins into components with higher biological activity. The prepared ginseng extract composition can not only treat alcoholic fatty liver, but also has a preventive effect on alcoholic fatty liver. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 It is a comparison chart of serum biochemical indicators in each prevention group.

[0038] Figure 2 It is a comparison chart of liver tissue lipid metabolism indicators in each prevention group.

[0039] Figure 3 It is a comparison chart of lipid peroxidation indexes in liver tissue of each prevention group.

[0040] Figure 4 It is a comparison chart of body weight and liver weight changes in each prevention group.

[0041] Figure 5 Figure 2 is the liver anatomy of each prevention group.

[0042] Figure 6 These are HE staining images of liver tissues in each prevention group.

[0043] Figure 7 It is a comparison chart of serum biochemical indicators in each treatment group.

[0044] Figure 8 It is a comparison chart of liver tissue lipid metabolism indicators in each treatment group.

[0045] Figure 9 It is a comparison chart of lipid peroxidation indexes in liver tissue of each treatment group.

[0046] Figure 10 It is a comparison chart of the changes in body weight and liver weight in each treatment group.

[0047] Figure 11 Figure 4 is an anatomical diagram of the liver in each treatment group.

[0048] Figure 12HE staining images of liver tissues in each treatment group. DETAILED DESCRIPTION

[0049] To make the purpose, technical solutions, and advantages of the embodiments of the present disclosure more clear, the technical solutions of the embodiments of the present disclosure are clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present disclosure, not all of the embodiments. Based on the described embodiments of the present disclosure, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0050] It should be understood that, under the premise of no conflict, any and all embodiments of the present invention can be combined with the technical features in any other embodiment or multiple other embodiments to obtain additional embodiments. The present invention includes such combinations to obtain additional embodiments.

[0051] Unless otherwise specified, all technical and scientific terms used herein have the standard meanings in the art to which the claimed subject matter belongs. If there are multiple definitions for a term, the definition herein shall prevail.

[0052] Ginseng, a widely used and long-standing medicinal herb, has the ability to sober up. Research has shown that ginsenosides possess numerous therapeutic benefits, including antioxidant, anti-inflammatory, vasodilator, anti-allergic, and anti-diabetic properties, without toxic side effects. While ginseng's numerous benefits and potential for treating alcoholic fatty liver disease are known, the challenge remains to harness its components to enhance their effectiveness and focus on treating this condition.

[0053] Ginsenosides are a composite material extracted from ginseng. Their composition is complex, and the components extracted from different parts of ginseng are different. More than 40 ginsenosides have been isolated from ginseng roots in the existing technology, and the composition of ginseng has not yet been fully explored.

[0054] The project team serendipitously discovered that mixing a slurry of fresh ginseng stems and leaves with saponins extracted from dried ginseng overnight and then recovering the saponins from the mixture was more effective in treating and preventing alcoholic fatty liver disease than extracting the whole ginseng root directly. They hypothesized that enzymes in fresh ginseng could partially convert the saponins extracted from dried ginseng, making the resulting mixture more effective in treating and preventing alcoholic fatty liver disease.

[0055] Based on this, the project team continued to experiment with different parts of ginseng under different conditions and adjusted a method for preparing a ginseng extract composition, which includes the following steps:

[0056] S1: Extraction of crude enzyme extract from fresh ginseng with fresh stems and leaves.

[0057] S2: Extracting total saponins from the main root of ginseng.

[0058] S3: Extract total saponins from ginseng root fibers.

[0059] S4: dissolving and mixing the crude enzyme extract, the main root total saponins and the fibrous root total saponins with a buffer solution, and converting them at a temperature suitable for the enzyme for a preset time to obtain a crude production system.

[0060] The water and water-soluble impurities in the crude product system are removed through purification in step S5 and refinement in step S6 to obtain a ginseng extract composition.

[0061] The prepared ginseng extract composition has a total ginsenoside content of ≥90wt%, and contains ginsenosides S-Rg2, R-Rg2, S-Rg3, R-Rg3, Rg4, Rg5, Rg6, S-Rh1, R-Rh1, S-Rh2, R-Rh2, Rh3, Rh4, Rk1, Rk2, and Rk3. It should be noted that ginsenosides are inherently complex and have not been fully explored. It is also difficult to qualitatively and quantitatively determine all the components of the ginseng extract composition after transformation. It is unclear whether the benefit of transformation in treating and preventing alcoholic fatty liver disease is due to the production of more unknown substances or a change in the specific saponin ratio. This only indicates that the ginseng extract composition prepared in this application can be measured to meet this condition, and it cannot be considered a characterization of the active ingredients (ingredients that are more beneficial for treating and preventing alcoholic fatty liver disease). The fact that compositions obtained by other extraction methods meet these conditions does not guarantee that they will achieve the same effect as the composition prepared by the preparation method of this application.

[0062] The ginseng extract composition can be used as a general food, health supplement, or medicine. For example, it can be made into medicinal wine, capsules, and snacks. The application of the ginseng extract composition in food or beverages facilitates the prevention or improvement of alcoholic fatty liver disease in daily life, increasing its feasibility and popularity.

[0063] The ginseng extract composition in the following experiments was prepared by the following steps. For ease of description, the concentration of alcohol is by volume.

[0064] Enzyme extraction: Take fresh ginseng with fresh stems and leaves and grind it into a homogenous slurry at below 30℃, add 1-2 times the weight of fresh ginseng water to mix, stir thoroughly and centrifuge, retain the supernatant, continue to mix it with the precipitate with appropriate amount of water, centrifuge, combine the supernatants from multiple times, concentrate under reduced pressure at 30-40℃, mix the concentrate with 3 times 95% edible alcohol, centrifuge, and the obtained precipitate is the crude enzyme extract.

[0065] Extraction of total saponins from ginseng taproot: Grind the ginseng taproot, extract with 8-10 times the amount of water at 85-95°C for 1 hour, repeat the extraction three times, combine the extracts, adsorb on a macroporous adsorption resin column, wash away impurities with water, elute the saponins with 70%-90% alcohol, and concentrate and dry to obtain total saponins from ginseng taproot.

[0066] Extraction of total saponins from ginseng fibrous roots: Extract total saponins according to the method of ginseng taproot.

[0067] Transformation: Mix the total saponins from the main root of ginseng and the total saponins from the fibrous root of ginseng, add 10 times the pH 5.5-7.0 phosphate buffer to fully dissolve them, dissolve the crude enzyme extract in 10 times the pH 5.5-7.0 phosphate buffer, and then mix with the saponin solution (first solution) in an equal weight. Keep warm at 40°C for 48-96 hours with stirring at 80-120 rpm.

[0068] Purification: After the insulation is completed, the mixture is concentrated under reduced pressure to one-tenth of the volume, and then 3 times 95% edible alcohol is added to fully dissolve it. The supernatant is evaporated to dryness to obtain a crude product.

[0069] Refining: The crude product is dissolved in 40% to 50% edible alcohol and adsorbed on a macroporous adsorption resin column. After adsorption, water is used to wash away water-soluble impurities such as salt and sugar, and then eluted with 85% to 95% alcohol. The eluted solution is concentrated and dried to obtain the ginseng extract composition.

[0070] In addition, the ginseng extract control required for the control experiment was prepared as follows:

[0071] Enzyme extraction: Take fresh ginseng roots without stems and leaves and grind them into a homogenate at below 30℃, add 1-2 times the weight of fresh ginseng water and mix, stir thoroughly and centrifuge, retain the supernatant, continue to mix it with the precipitate with appropriate amount of water, centrifuge, combine the supernatants from multiple times, concentrate under reduced pressure at 30-40℃, mix the concentrate with 3 times 95% edible alcohol, centrifuge, and the obtained precipitate is the crude enzyme extract.

[0072] Extraction of total saponins from ginseng roots: Crush the main root of ginseng, extract with 8-10 times the amount of water at 85-95°C for 1 hour, repeat the extraction three times, combine the extracts, adsorb on a macroporous adsorption resin column, wash away impurities with water, elute the saponins with 70%-90% alcohol, and concentrate and dry to obtain total saponins from the main root of ginseng.

[0073] Transformation: Dissolve the total saponins from the ginseng taproot in 10-fold phosphate buffer (pH 5.5-7.0). Dissolve the crude enzyme extract in 10-fold phosphate buffer (pH 5.5-7.0). Mix the extract with an equal weight of the saponin solution (first solution). Keep the mixture at 40°C with stirring at 80-120 rpm for 48-96 hours.

[0074] Purification: After the insulation is completed, the mixture is concentrated under reduced pressure to one-tenth of the volume, and then 3 times 95% edible alcohol is added to fully dissolve it. The supernatant is evaporated to dryness to obtain a crude product.

[0075] Refining: Dissolve the crude product in 40%~50% edible alcohol and adsorb it on a macroporous adsorption resin column. After adsorption, wash away water-soluble impurities such as salt and sugar with water, then elute with 85%~95% alcohol. Concentrate and dry the eluted solution to obtain the ginseng extract reference.

[0076] Experimental Preparation

[0077] Reagents: 0.9% saline, acetic acid, 4% paraformaldehyde, anhydrous ethanol, 95% ethanol, silybin, mouse alcohol liquid feed, mouse alcohol-free control liquid feed, maltodextrin, aspartate aminotransferase (AST / GOT) kit, alanine aminotransferase (ALT / GOT) kit, triglyceride (TG) kit, total cholesterol (TC) kit, reduced glutathione (GSH) ratio kit, malondialdehyde (MDA) kit, and BCA protein concentration assay kit, etc.

[0078] To prepare L-RGG solution, the ginseng extract composition prepared above was dissolved in 20% ethanol solution to prepare a 20 mg / kg solution.

[0079] To prepare H-RGG solution, the ginseng extract composition prepared above was dissolved in 20% ethanol solution to prepare a 40 mg / kg solution.

[0080] To prepare the RT solution, dissolve the ginseng extract control prepared above in 20% ethanol solution to make a 40 mg / kg solution.

[0081] Instruments: constant temperature water bath, microplate reader, high-speed low-temperature centrifuge, homogenizer, etc.

[0082] Experimental mice: healthy SPF-grade C57BL / 6 J male mice (weighing 20-22 g)

[0083] Statistical analysis of experimental data was performed using GraphPad Prism 10.0 software. Final data are expressed as mean ± standard deviation, and differences between groups were compared using one-way analysis of variance. Statistical significance is defined as follows: * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001; **** indicates p < 0.0001.

[0084] Experimental process

[0085] 1. After five days of adaptive feeding, mice in the blank prevention group were fed an alcohol-free control liquid diet. Starting from the first day of feeding with the alcohol-free control liquid diet, they were gavaged with 0.1 ml of normal saline every day. After five days of adaptive feeding, mice in the blank treatment group were fed an alcohol-free control liquid diet. On the tenth day of feeding with the alcohol-free control liquid diet, they were gavaged with 0.1 ml of normal saline.

[0086] 2. After five days of adaptive feeding, mice in the prevention group were fed a 1% to 5% (vol / vol) alcohol liquid diet at increasing concentrations (1% on the first day, 2% on the second day, 3% on the third day, 4% on the fourth day, and 5% on the fifth day). They continued to be fed a 5% (vol / vol) alcohol liquid diet. Starting from the first day of feeding with the alcohol liquid diet, they were gavaged with 0.1 ml of normal saline daily. After five days of adaptive feeding, mice in the treatment group were fed a 1% to 5% (vol / vol) alcohol liquid diet at increasing concentrations. They continued to be fed a 5% (vol / vol) alcohol liquid diet. On the sixth day of feeding with the 5% alcohol liquid diet (equivalent to the tenth day of feeding with the alcohol liquid diet), they were gavaged with 0.1 ml of normal saline.

[0087] 3. After five days of adaptive feeding, mice in the silibinin prevention group were fed a diet containing increasing concentrations of 1% to 5% (vol / vol) alcohol, followed by a 5% (vol / vol) alcohol diet. Starting on the first day of feeding with the alcohol diet, mice were gavaged daily with 0.1 ml of a silibinin solution (50 mg / kg). After five days of adaptive feeding, mice in the silibinin treatment group were fed a diet containing increasing concentrations of 1% to 5% (vol / vol) alcohol, followed by a 5% (vol / vol) alcohol diet. On the sixth day of feeding with the 5% alcohol diet, mice were gavaged with 0.1 ml of a silibinin solution (50 mg / kg).

[0088] 4. After five days of adaptive feeding, mice in the L-RGG prevention group were fed a 1% to 5% (vol / vol) alcohol liquid diet with increasing concentrations. Once the mice adapted to the alcohol concentration, they continued to be fed a 5% (vol / vol) alcohol liquid diet. Starting on the first day of feeding with the alcohol liquid diet, they were gavaged daily with 0.1 ml of L-RGG solution (20 mg / kg). After five days of adaptive feeding, mice in the L-RGG treatment group were fed a 1% to 5% (vol / vol) alcohol liquid diet with increasing concentrations. Once the mice adapted to the alcohol concentration, they continued to be fed a 5% (vol / vol) alcohol liquid diet. On the sixth day of feeding with the 5% alcohol liquid diet, they were gavaged with 0.1 ml of L-RGG solution (20 mg / kg).

[0089] 5. After five days of adaptive feeding, mice in the H-RGG prevention group were fed a 1% to 5% (vol / vol) alcohol liquid diet with increasing concentrations. Once the mice adapted to the alcohol concentration, they continued to be fed a 5% (vol / vol) alcohol liquid diet. Starting from the first day of feeding with the alcohol liquid diet, they were gavaged daily with 0.1 ml of H-RGG solution (40 mg / kg). After five days of adaptive feeding, mice in the H-RGG treatment group were fed a 1% to 5% (vol / vol) alcohol liquid diet with increasing concentrations. Once the mice adapted to the alcohol concentration, they continued to be fed a 5% (vol / vol) alcohol liquid diet. On the sixth day of feeding with the 5% alcohol liquid diet, they were gavaged with 0.1 ml of H-RGG solution (20 mg / kg).

[0090] 6. After five days of adaptive feeding, mice in the RT prevention group were fed a 1% to 5% (vol / vol) alcohol liquid diet at increasing concentrations, followed by a 5% (vol / vol) alcohol liquid diet. Starting on the first day of feeding with the alcohol liquid diet, they were gavaged daily with 0.1 ml of a ginsenoside solution (40 mg / kg). After five days of adaptive feeding, mice in the RT treatment group were fed a 1% to 5% (vol / vol) alcohol liquid diet at increasing concentrations, followed by a 5% (vol / vol) alcohol liquid diet. On the sixth day of feeding with the 5% alcohol liquid diet, they were gavaged with 0.1 ml of a ginsenoside solution (40 mg / kg).

[0091] 7. On the eleventh day of the experiment (the sixteenth day if adaptive feeding is included), all mice were gavaged with 95% ethanol (5 g / kg). Mice in each group died suddenly 9 hours after gavage.

[0092] 8. Blood was collected by enucleation. Immediately after enucleation, the entire liver was excised and rinsed several times with pre-chilled saline to remove any residual blood for weighing. The largest right lobe of each liver was immersed in 4% paraformaldehyde for preparation of tissue sections for histopathological evaluation. The remaining liver tissue was prepared into the corresponding liver homogenate for testing according to the corresponding kit instructions.

[0093] The experimental results are as follows Figures 1 to 12 In the figure, the blank group is represented by Control, the model group is represented by Model, the silybin group is represented by Silybin, the L-RGG group is represented by L-RGG, the H-RGG group is represented by H-RGG, and the ginseng whole extract group is represented by RT.

[0094] Reference Figures 1 to 6Results from the prevention groups showed that a 40 mg / kg dose of RGG effectively inhibited the elevation of triglycerides (TG), total cholesterol (TC), malondialdehyde (MDA), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) in the mouse liver; effectively promoted glutathione (GSH) synthesis; and curbed weight loss and liver weight gain in mice. HE staining results showed that RGG significantly reduced the formation of fat particles in the liver. RGG demonstrated a strong preventive effect against alcoholic liver disease, but this was not dose-dependent. The control ginseng extract composition 2 showed no significant preventive effect, with no statistically significant effect observed, indicating that the crude enzymes in fresh ginseng root alone are incapable of converting the active ingredients.

[0095] Reference Figures 7 to 12 The experimental results of each treatment group showed that high-dose RGG (the ginseng extract composition of the present application) effectively suppressed the increase in triglyceride (TG), total cholesterol (TC), malondialdehyde (MDA), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) in the mouse liver; effectively promoted the synthesis of glutathione (GSH); and curbed the decrease in mouse body weight and the increase in liver weight. HE staining results showed that RGG significantly reduced the formation of fat particles in the liver. It has a good therapeutic effect on alcoholic liver disease, and a dose-dependent relationship is observed. The therapeutic effect of the ginseng extract control group was not obvious, and no statistically significant effect was found, indicating that the crude enzymes in fresh ginseng root alone cannot be converted into effective ingredients.

[0096] Throughout this specification, reference to terms such as "one embodiment," "certain embodiments," "illustrative embodiments," "examples," "specific examples," or "some examples" means that the specific features, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, illustrative uses of these terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

[0097] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications are also considered to be within the scope of protection of the present invention.

Claims

1. A method for preparing a ginseng extract composition, characterized in that: The following steps are involved: Extracting crude enzyme extract from fresh ginseng with fresh stems and leaves; Extracting taproot total saponins from ginseng taproot; Extracting total saponins from ginseng root fibers; dissolving and mixing the crude enzyme extract, the main root total saponins, and the fibrous root total saponins with a buffer solution, and converting them at a temperature suitable for the enzyme for a predetermined time to obtain a crude production system; removing water and water-soluble impurities from the crude product system to obtain the ginseng extract composition; The step of extracting the enzyme-containing crude extract from fresh ginseng containing fresh stems and leaves comprises: The fresh ginseng containing fresh stems and leaves is crushed into a pulp at below 30°C, water is added and the mixture is centrifuged, the supernatants are combined, and the mixture is concentrated under reduced pressure at 30°C to 40°C to obtain a concentrate, the concentrate is mixed with 95% edible alcohol in an amount three times the volume of the concentrate, and the insoluble matter is collected by centrifugation to obtain the crude enzyme extract; The main root total saponins and the fibrous root total saponins are extracted according to the following steps: The root of the relevant part is crushed, and extracted with 8 to 10 times the amount of water by weight at 85°C to 95°C for at least 1 hour, and the extraction is repeated multiple times. The extracts are combined, and the extracts are adsorbed with a macroporous adsorption resin. The macroporous adsorption resin is rinsed with water to remove impurities, and then the macroporous adsorption resin is eluted with 70% to 90% edible alcohol. Finally, the eluate is concentrated and dried to obtain saponins from the relevant part. The requirement for converting at the enzyme suitable temperature for a preset time is: keeping the temperature at 40° C. for 48 h to 96 h under stirring at 80 rpm to 120 rpm.

2. The method for preparing the ginseng extract composition according to claim 1, wherein: The step of dissolving and mixing the crude enzyme extract, the main root total saponins and the fibrous root total saponins with a buffer solution comprises: Mixing the main root total saponins and the fibrous root total saponins to obtain a first mixture, and dissolving the first mixture with the buffer solution 10 times the mass of the first mixture to obtain a first solution; dissolving the crude enzyme extract with the buffer solution that is 10 times the mass of the crude enzyme extract to obtain a second solution; mixing equal masses of the first solution and the second solution; The buffer solution is a phosphate buffer solution with a pH of 5.5 to 7.

0.

3. The method for preparing the ginseng extract composition according to claim 2, wherein: In the first mixture, the mass ratio of the main root total saponins to the fibrous root total saponins is 2.6:

1.

4. The method for preparing the ginseng extract composition according to claim 1, wherein: The step of removing water and water-soluble impurities in the crude product system comprises: The crude product system was concentrated under reduced pressure to one tenth of its original volume to obtain a crude product concentrate, 3 times the volume of 95% edible alcohol as that of the crude product concentrate was added to the crude product concentrate, and then the supernatant was evaporated to dryness to obtain a crude product; The crude product is dissolved in 40% to 50% edible alcohol and adsorbed on a macroporous adsorption resin. The macroporous adsorption resin is rinsed with water to remove impurities. The macroporous adsorption resin is then eluted with 85% to 95% edible alcohol. Finally, the eluate is concentrated and dried.

5. A ginseng extract composition for preventing alcoholic fatty liver disease, characterized in that: The ginseng extract composition is prepared by the preparation method of any one of claims 1 to 4.

6. The ginseng extract composition according to claim 5, characterized in that The total ginsenoside content of Panax not exceeding 90wt%, comprising ginsenosides S-Rg2, R-Rg2, S-Rg3, R-Rg3, Rg4, Rg5, Rg6, S-Rh1, R-Rh1, S-Rh2, R-Rh2, Rh3, Rh4, Rk1, Rk2 and Rk3.

7. Use of the ginseng extract composition according to any one of claims 5 or 6 in the preparation of a medicine for preventing alcoholic fatty liver.

Images