A cough relieving and asthma relieving composition

Abstract

The application discloses a cough relieving and asthma relieving composition and belongs to the field of traditional Chinese medicines. The cough relieving and asthma relieving composition is made of traditional Chinese medicine fermentation granules, bitter apricot kernels, perilla leaves and radix peucedani. The traditional Chinese medicine fermentation granules are made of pericarpium citri reticulatae, platycodon grandiflorum and glycyrrhiza uralensis as raw materials. Cellulase is used to enzymatically hydrolyze the raw materials to obtain an enzymatic hydrolysis liquid. Then, lactobacillus plantarum, lactobacillus johnsonii and rhodotorula mucilaginosa are used to mix ferment the enzymatic hydrolysis liquid to obtain a fermentation liquid. The fermentation liquid is sterilized, filtered, dried and granulated to obtain the traditional Chinese medicine fermentation granules. The traditional Chinese medicine fermentation granules, the bitter apricot kernels, the perilla leaves and the radix peucedani are used in combination and have a synergistic effect, so that an excellent cough relieving and asthma relieving effect is achieved.

Description

Technical Field

[0001] The present application relates to the technical field of traditional Chinese medicine, and in particular to a cough and asthma relieving composition. Background Art

[0002] Xing Su San is composed of perilla leaves, pinellia, poria, peucedanum, bitter almond, bitter platycodon, fructus aurantii, tangerine peel, licorice, ginger, and jujube. It is mainly used to treat exogenous cold and dryness syndrome, aversion to cold without sweating, slight headache, cough with thin sputum, nasal congestion and dry throat, white tongue coating and stringy pulse. It is one of the ancient prescriptions recorded in Su Wen Zhi Zhen Yao Da Lun. Perilla leaves and bitter almond are the main ingredients, pinellia, platycodon, and fructus aurantii are the secondary ingredients, pinellia, tangerine peel, poria, ginger, and jujube are the auxiliary ingredients, and licorice is the assistant ingredient. It has certain cough, phlegm and asthma-relieving effects, but the decoction preparation of traditional Chinese medicine makes it impossible to take it in time when symptoms appear, and the decoction time and the degree of decoction may affect the efficacy, which is extremely inconvenient.

[0003] With the advancement of science and technology, fermentation technology is rapidly evolving. The synergistic effects of multiple strains in multi-strain mixed fermentation are expected to further amplify the efficacy of formulas. Furthermore, compared to traditional Chinese medicine decoctions, granular preparations offer advantages such as portability, precise dosage, enhanced stability, and long-term room temperature storage. Therefore, the use of multi-strain mixed fermentation to improve existing Chinese medicine formulas and the adoption of granules as an alternative to traditional Chinese medicine dosage forms hold significant research value. Summary of the Invention

[0004] The present invention aims to provide a cough and asthma relieving composition, which comprises the following steps: using a multi-strain mixed fermentation method to ferment tangerine peel, platycodon grandiflorum and liquorice, and then simply preparing the fermentation product with bitter almond, perilla leaf and peucedanum peucedanum to obtain a composition capable of improving respiratory diseases. The composition has high safety, and the fermentation product obtained by fermenting tangerine peel, platycodon grandiflorum and liquorice is compounded with bitter almond, perilla leaf and peucedanum peucedanum to achieve synergistic effect, thereby having excellent cough and asthma relieving effects.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] In a first aspect, the present invention provides a method for preparing Chinese medicine fermented granules, wherein the preparation raw materials include the following ingredients: dried tangerine peel, platycodon grandiflorum, and liquorice, with a mass ratio of 1:1:0.3-0.4.

[0007] The specific preparation steps of the Chinese medicine fermentation granules are as follows:

[0008] S1: Grind dried tangerine peel, platycodon grandiflorum, and liquorice together, and pass through a 400-600 mesh sieve to obtain a mixed powder;

[0009] S2: Add sterile deionized water 10 times the mass of the mixed powder to the mixed powder, stir evenly to obtain a mixed solution;

[0010] S3: adding cellulase to the mixed solution for enzymolysis, wherein the amount of cellulase added is 5-7 wt % of the mixed solution, the enzymolysis temperature is 30-35° C., and the enzymolysis time is 3-6 h. After the enzymolysis is completed, filtering is performed to obtain an enzymolysis solution;

[0011] S4: adding 9-12 wt% glucose and 3 wt% peptone to the enzymatic hydrolysate, mixing well, sterilizing the enzymatic hydrolysate at 105°C for 40 min, and filtering to obtain a filtrate;

[0012] S5: inoculating Lactobacillus plantarum, Lactobacillus johnsonii and Rhodotorula glutinosus into the filtrate and performing mixed fermentation. After the fermentation, sterilizing at high temperature and filtering to obtain a Chinese medicine fermentation liquid, wherein the mass ratio of Lactobacillus plantarum, Lactobacillus johnsonii and Rhodotorula glutinosus is 1:0.5-1:1, the total amount of bacteria is 8-10wt% of the filtrate, the fermentation time is 12-16h, the fermentation temperature is 28-30°C, the fermentation pH is 5.8-7.1, and the ventilation rate is 3L / min;

[0013] S6: Concentrating the Chinese medicine fermentation liquid, spray drying and granulating to obtain Chinese medicine fermentation granules.

[0014] Preferably, the mass ratio of Lactobacillus plantarum, Lactobacillus johnsonii and Rhodotorula glutinosae is 1:0.7:1.

[0015] In a second aspect, the present invention provides a cough and asthma relieving composition, wherein the composition is made of the following components in parts by weight:

[0016] 5.4-5.6 parts of Chinese herbal fermented granules;

[0017] 1-1.3 parts bitter almonds;

[0018] 1-1.3 parts of perilla leaves;

[0019] Peucedanum 1-1.3 parts;

[0020] 90-92 parts of sucrose.

[0021] Preferably, the composition is made from the following components in parts by weight:

[0022] 5.4-5.5 parts of Chinese herbal fermented granules;

[0023] 1-1.15 parts bitter almonds;

[0024] 1-1.15 parts of perilla leaves;

[0025] Peucedanum 1-1.15 parts;

[0026] Sucrose 91-92 parts.

[0027] Most preferably, the composition is made from the following components in parts by weight:

[0028] 5.5 parts of Chinese herbal fermented granules;

[0029] 1.15 parts bitter almonds;

[0030] 1.15 parts of perilla leaves;

[0031] Peucedanum 1.15 parts;

[0032] 91.05 parts of sucrose.

[0033] In a third aspect, the present invention provides a method for preparing an antitussive and antiasthmatic composition, the specific steps of which are as follows:

[0034] T1: Crush bitter almonds, soak in water for 20-26 hours, steam distill, collect the distillate, add 1-2 v / v% 90-95 v / v% ethanol to the distillate, perform secondary distillation, collect the secondary distillate, dilute the secondary distillate with ultrapure water to a concentration of 0.3 mg of hydrocyanic acid per 1 ml, and seal for later use;

[0035] T2: Extract the extracts of Perilla frutescens leaves and Peucedanum peucedanum by steam distillation, seal and set aside, wherein the solid-liquid ratio of the raw materials to water is 1:20;

[0036] T3: dissolving the Chinese medicinal fermentation granules in the bitter almond double distilled dilution solution to obtain a mixed solution;

[0037] T4: Evenly mix the perilla leaf extract, peucedanum peucedanum extract, sucrose, and the mixed solution obtained in step T3, cool, and spray-dry to obtain finished granules;

[0038] T5: Divide the finished granules into smaller pieces, seal them tightly, and store them at room temperature.

[0039] Compared with the prior art, the present invention has the following beneficial effects: It provides a cough and asthma relieving composition, which is made from traditional Chinese medicine fermented granules, bitter almonds, perilla leaves, and peucedanum. The traditional Chinese medicine fermented granules are made from dried tangerine peel, platycodon, and licorice. The raw materials are first enzymatically hydrolyzed with cellulase to obtain an enzymatic hydrolyzate, which is then mixed and fermented with Lactobacillus plantarum, Lactobacillus johnsonii, and Rhodotorula glutinosae to obtain a fermentation broth. The mixed fermentation broth is then sterilized, filtered, dried, and granulated to obtain the traditional Chinese medicine fermented granules. Among them, the secondary metabolites of Lactobacillus plantarum can improve the biological barrier and enhance immunity; Lactobacillus johnsonii and its secondary metabolites may be one of the bacteria involved in respiratory immune protection; yeast can synergize and complement the nutrition of Lactobacillus strains, promoting the proliferation of Lactobacillus, and the carotenoids in Rhodotorula glutinosae also help neutralize free radicals and reduce cell damage, thereby protecting respiratory and lung cells. The study found that the fermentation products obtained by mixed fermentation of tangerine peel, platycodon and licorice with Lactobacillus plantarum, Lactobacillus johnsonii and Rhodotorula, combined with bitter almond, perilla leaf and peucedanum scutellariae, have better antitussive and antiasthmatic effects than the simple combination of tangerine peel, platycodon, licorice with bitter almond, perilla leaf and peucedanum scutellariae. DETAILED DESCRIPTION

[0040] In order to better understand the present invention, the present invention is further described below in conjunction with specific application examples, wherein the terms used in the application examples are for describing specific embodiments and do not constitute a limitation on the scope of protection of the present invention.

[0041] In the following examples, the experimental methods without specific conditions are generally based on conventional conditions or the conditions recommended by the manufacturer. Unless otherwise specified, the percentages and parts by mass are calculated.

[0042] Some of the raw materials and their sources are as follows:

[0043] Tangerine peel: purchased from Bozhou Ruantang Pharmaceutical Co., Ltd.

[0044] Platycodon grandiflorum: purchased from Bozhou Ruantang Pharmaceutical Co., Ltd.

[0045] Licorice: purchased from Bozhou Ruantang Pharmaceutical Co., Ltd.

[0046] Bitter almonds: purchased from Bozhou Ruantang Pharmaceutical Co., Ltd.

[0047] Perilla leaves: purchased from Bozhou Ruantang Pharmaceutical Co., Ltd.

[0048] Peucedanum peucedanum: purchased from Bozhou Ruantang Pharmaceutical Co., Ltd.

[0049] Cellulase: purchased from Xiasheng Group, Xiasheng Cellulase (CEL-01 model), product number: FDY-2243, enzyme activity: 11000u / mL;

[0050] Lactobacillus plantarum: purchased from China General Microorganism Collection Center, with the collection number CGMCC 1.568;

[0051] Lactobacillus johnsonii: purchased from China General Microorganism Collection Center, with the accession number CGMCC 1.10778;

[0052] Rhodotorula glutinosae: purchased from China General Microorganism Collection Center, with the collection number CGMCC 2.5690.

[0053] The preparation process of Chinese medicine fermentation granules has the following specific steps:

[0054] Chinese herbal fermented granules 1:

[0055] S1: Grind dried tangerine peel, platycodon grandiflorum, and liquorice together, and pass through a 500-mesh sieve to obtain a mixed powder, wherein the mass ratio of dried tangerine peel, platycodon grandiflorum, and liquorice is 1:1:0.4;

[0056] S2: Add sterile deionized water 10 times the mass of the mixed powder to the mixed powder, stir evenly to obtain a mixed solution;

[0057] S3: adding cellulase to the mixed solution for enzymatic hydrolysis, wherein the amount of cellulase added is 6 wt % of the mixed solution, the enzymatic hydrolysis temperature is 33° C., and the enzymatic hydrolysis time is 5 h. After the enzymatic hydrolysis is completed, filtering is performed to obtain an enzymatic hydrolyzate;

[0058] S4: adding 10 wt% glucose and 3 wt% peptone to the enzymatic hydrolysate, mixing well, sterilizing the enzymatic hydrolysate at 105°C for 40 min, and filtering to obtain a filtrate;

[0059] S5: inoculating Lactobacillus plantarum, Lactobacillus johnsonii and Rhodotorula glutinosus into the filtrate and performing mixed fermentation. After the fermentation, the mixture was sterilized at high temperature and filtered to obtain a Chinese medicine fermentation liquid, wherein the mass ratio of Lactobacillus plantarum, Lactobacillus johnsonii and Rhodotorula glutinosus was 1:0.7:1, the total amount of bacteria was 9 wt % of the filtrate, the fermentation time was 14 h, the fermentation temperature was 29° C., the fermentation pH was 6.5, and the sterile air ventilation rate was 3 L / min;

[0060] S6: Concentrating the Chinese medicine fermentation liquid to 20% of the original volume, spray drying and granulating to obtain Chinese medicine fermentation granules.

[0061] Chinese herbal fermented granules 2:

[0062] S1: Grind dried tangerine peel, platycodon grandiflorum, and liquorice together, and pass through a 400-mesh sieve to obtain a mixed powder, wherein the mass ratio of dried tangerine peel, platycodon grandiflorum, and liquorice is 1:1:0.3;

[0063] S2: Add sterile deionized water 10 times the mass of the mixed powder to the mixed powder, stir evenly to obtain a mixed solution;

[0064] S3: adding cellulase to the mixed solution for enzymatic hydrolysis, wherein the amount of cellulase added is 7 wt % of the mixed solution, the enzymatic hydrolysis time is 3 h, the enzymatic hydrolysis temperature is 30° C. After the enzymatic hydrolysis is completed, filtering is performed to obtain an enzymatic hydrolyzate;

[0065] S4: adding 12 wt% glucose and 3 wt% peptone to the enzymatic hydrolysate, mixing well, sterilizing the enzymatic hydrolysate at 105°C for 40 min, and filtering to obtain a filtrate;

[0066] S5: inoculating Lactobacillus plantarum, Lactobacillus johnsonii and Rhodotorula glutinosus into the filtrate and performing mixed fermentation. After the fermentation, the mixture was sterilized at high temperature and filtered to obtain a Chinese medicine fermentation broth, wherein the mass ratio of Lactobacillus plantarum, Lactobacillus johnsonii and Rhodotorula glutinosus was 1:0.5:1, the total amount of bacteria was 8 wt % of the filtrate, the fermentation time was 16 h, the fermentation temperature was 28° C., the fermentation pH was 5.8, and the sterile air ventilation rate was 3 L / min;

[0067] S6: Concentrating the Chinese medicine fermentation liquid to 30% of the original volume, spray drying and granulating to obtain Chinese medicine fermentation granules.

[0068] Chinese herbal fermented granules 3:

[0069] S1: Grind dried tangerine peel, platycodon grandiflorum and liquorice together, and pass through a 600-mesh sieve to obtain a mixed powder, wherein the mass ratio of dried tangerine peel, platycodon grandiflorum and liquorice is 1:1:0.4;

[0070] S2: Add sterile deionized water 10 times the mass of the mixed powder to the mixed powder, stir evenly to obtain a mixed solution;

[0071] S3: adding cellulase to the mixed solution for enzymolysis, wherein the amount of cellulase added is 5 wt % of the mixed solution, the enzymolysis time is 6 h, the enzymolysis temperature is 35°C, and after the enzymolysis is completed, filtering is performed to obtain an enzymolysis solution;

[0072] S4: adding 9 wt% glucose and 3 wt% peptone to the enzymatic hydrolysate, mixing well, sterilizing the enzymatic hydrolysate at 105°C for 40 min, and filtering to obtain a filtrate;

[0073] S5: inoculating Lactobacillus plantarum, Lactobacillus johnsonii and Rhodotorula glutinosus into the filtrate and performing mixed fermentation. After the fermentation, the mixture was sterilized at high temperature and filtered to obtain a Chinese medicine fermentation liquid, wherein the mass ratio of Lactobacillus plantarum, Lactobacillus johnsonii and Rhodotorula glutinosus was 1:1:1, the total amount of bacteria was 10 wt % of the filtrate, the fermentation time was 12 h, the fermentation temperature was 30° C., the fermentation pH was 7.1, and the sterile air ventilation rate was 3 L / min;

[0074] S6: Concentrating the Chinese medicine fermentation liquid to 10% of the original volume, spray drying and granulating to obtain Chinese medicine fermentation granules.

[0075] Chinese medicine fermented granules①:

[0076] The difference from the Chinese medicine fermentation granules 1 is that the mass ratio of Lactobacillus plantarum, Lactobacillus johnsonii, and Rhodotorula glutinosus is 1:0:1, and the remaining steps are the same as those of the Chinese medicine fermentation granules 1.

[0077] Chinese medicine fermented granules②:

[0078] The difference from the Chinese medicine fermentation granules 1 is that the mass ratio of Lactobacillus plantarum, Lactobacillus johnsonii, and Rhodotorula glutinosus is 1:0.7:0, and the remaining steps are the same as those of the Chinese medicine fermentation granules 1.

[0079] Chinese medicine fermented granules ③:

[0080] The difference from the Chinese medicine fermentation granules 1 is that the mass ratio of Lactobacillus plantarum, Lactobacillus johnsonii, and Rhodotorula glutinosus is 0:0.7:1, and the remaining steps are the same as those of the Chinese medicine fermentation granules 1.

[0081] Chinese medicine fermented granules ④:

[0082] The difference from the Chinese medicine fermentation granules 1 is that the steps S4-S5 are not included, the enzymatic hydrolysate obtained in the step S3 is subjected to the step S6, and the remaining steps are the same as those of the Chinese medicine fermentation granules 1.

[0083] Chinese medicine fermented granules⑤:

[0084] Different from the Chinese medicine fermentation granules 1, the steps S3-S5 are not included. The mixed solution obtained in the step S2 is subjected to hot reflux extraction at an extraction temperature of 65°C, an extraction time of 5 hours, and four extractions. The filtrates are combined, and then the filtrate is subjected to step S6.

[0085] Chinese medicine fermented granules ⑥:

[0086] Different from the Chinese medicine fermentation granules 1, the S3 enzymatic hydrolysis step is not included, the mixed liquid obtained in S2 is directly subjected to the S4 step, and the remaining steps are the same as those of the Chinese medicine fermentation granules 1.

[0087] Chinese medicine fermented granules ⑦:

[0088] The difference from the Chinese medicine fermentation granules 1 is that only red yeast rice is used, and the remaining steps are the same as those of the Chinese medicine fermentation granules 1.

[0089] Chinese herbal fermented granules⑧:

[0090] The difference from the Chinese medicine fermented granules 1 is that only Lactobacillus johnsonii is used, and the remaining steps are the same as those of the Chinese medicine fermented granules 1.

[0091] Chinese medicine fermented granules ⑨:

[0092] The difference from the Chinese medicine fermentation granules 1 is that only Lactobacillus plantarum is used, and the remaining steps are the same as those of the Chinese medicine fermentation granules 1.

[0093] Traditional Chinese medicine fermented granules ⑩:

[0094] The difference from the Chinese medicine fermentation granules 1 is that the fermentation time in step S5 is 96 h, and the remaining steps are the same as those of the Chinese medicine fermentation granules 1.

[0095] A cough and asthma relieving composition, the weight proportion of which is shown in Table 1:

[0096] Table 1 Composition mass ratio

[0097] experimental group Chinese medicine fermented granules Chinese medicine fermented granule number bitter almonds Perilla leaves Peucedanum chinense Composition 1 5.5 1 1.15 1.15 1.15 91.05 Composition 2 5.4 1 1.3 1.3 1.3 90.7 Composition 3 5.6 1 1 1 1 91.4 Composition 4 5.5 2 1.15 1.15 1.15 91.05 Composition 5 5.5 3 1.15 1.15 1.15 91.05 Composition ① 5.5 ① 1.15 1.15 1.15 91.05 Composition ② 5.5 ② 1.15 1.15 1.15 91.05 Composition ③ 5.5 ③ 1.15 1.15 1.15 91.05 Composition ④ 5.5 ④ 1.15 1.15 1.15 91.05 Composition ⑤ 5.5 ⑤ 1.15 1.15 1.15 91.05 Composition ⑥ 5.5 ⑥ 1.15 1.15 1.15 91.05 Composition ⑦ 5.5 ⑦ 1.15 1.15 1.15 91.05 Composition ⑧ 5.5 ⑧ 1.15 1.15 1.15 91.05 Composition ⑨ 5.5 ⑨ 1.15 1.15 1.15 91.05 Composition ⑩ 5.5 ⑩ 1.15 1.15 1.15 91.05 Composition ⑪ 0 - 1.15 1.15 1.15 91.05 Composition ⑫ 5.5 1 0 0 0 91.05

[0098] In compositions ⑪-⑫, the missing components were replaced with equal amounts of sterile deionized water.

[0099] The preparation methods of the above compositions 1, 4, 5 and compositions ①-⑩ are as follows:

[0100] T1: Crush bitter almonds and soak them in water for 24 hours at a material-to-liquid ratio of 1:20. Steam distill the mixture at atmospheric pressure, collect the distillate, add 2 v / v% 93 v / v% ethanol to the distillate, and perform secondary distillation. Collect the secondary distillate and dilute it with ultrapure water to a concentration of 0.3 mg of hydrocyanic acid per 1 mL. Seal the mixture for later use.

[0101] T2: Extracting the extracts of Perilla frutescens leaves and Peucedanum peucedanum by steam distillation, sealing the extracts for later use, wherein the material-to-liquid ratio of the raw materials to water is 1:20, and the pressure is normal pressure;

[0102] T3: dissolving the Chinese medicine fermented granules in bitter almond secondary distillate to obtain solution B;

[0103] T4: uniformly mix the perilla leaf extract, peucedanum peucedanum extract, sucrose, and solution B, cool, and spray-dry to obtain granules B;

[0104] T5: Divide granules B into smaller packages, seal them tightly, and store them at room temperature.

[0105] The preparation method of composition 2 is as follows:

[0106] T1: Crush bitter almonds and soak them in water for 20 hours at a material-to-liquid ratio of 1:20. Steam distill the mixture under normal pressure and collect the distillate. Add 1 v / v% of 95 v / v% ethanol to the distillate and perform secondary distillation. Collect the secondary distillate and dilute it with ultrapure water to a concentration of 0.3 mg of hydrocyanic acid per 1 mL. Seal the mixture for later use.

[0107] T2: Extract the perilla leaf and peucedanum peucedanum extracts by steam distillation, seal and set aside, wherein the material-liquid ratio of the raw material to water is 1:20, and the pressure is normal pressure;

[0108] T3: dissolving the Chinese medicine fermented granules in bitter almond secondary distillate to obtain solution B;

[0109] T4: Evenly mix the perilla leaf extract, peucedanum peucedanum extract, sucrose, and solution B, cool, and spray-dry to obtain granules B;

[0110] T5: Divide granules B into smaller packages, seal them tightly, and store them at room temperature.

[0111] The preparation method of composition 3 is as follows:

[0112] T1: Crush bitter almonds and soak them in water for 26 hours at a material-to-liquid ratio of 1:20. Steam distill the mixture under normal pressure and collect the distillate. Add 2 v / v% of 90 v / v% ethanol to the distillate and perform secondary distillation. Collect the secondary distillate and dilute it with ultrapure water to a concentration of 0.3 mg of hydrocyanic acid per 1 mL. Seal the mixture for later use.

[0113] T2: Extract the perilla leaf and peucedanum peucedanum extracts by steam distillation, seal and set aside, wherein the material-liquid ratio of the raw material to water is 1:20, and the pressure is normal pressure;

[0114] T3: dissolving the Chinese medicine fermented granules in bitter almond secondary distillate to obtain solution B;

[0115] T4: Evenly mix the perilla leaf extract, peucedanum peucedanum extract, sucrose, and solution B, cool, and spray-dry to obtain granules B;

[0116] T5: Divide granules B into smaller packages, seal them tightly, and store them at room temperature.

[0117] Composition ⑪:

[0118] T1: Crush bitter almonds and soak them in water for 24 hours at a material-to-liquid ratio of 1:20. Steam distill the mixture at atmospheric pressure, collect the distillate, add 2 v / v% 93 v / v% ethanol to the distillate, and perform secondary distillation. Collect the secondary distillate and dilute it with ultrapure water to a concentration of 0.3 mg of hydrocyanic acid per 1 mL. Seal the mixture for later use.

[0119] T2: Extract the perilla leaf and peucedanum peucedanum extracts by steam distillation, seal and set aside, wherein the material-liquid ratio of the raw material to water is 1:20, and the pressure is normal pressure;

[0120] T3: Perilla leaf extract, Peucedanum peucedanum extract, sucrose, and bitter almond secondary distillate were mixed uniformly, cooled, and spray-dried to obtain granules B;

[0121] T4: Divide Granules B into smaller packages, seal tightly, and store at room temperature.

[0122] Composition ⑫:

[0123] T1: Dissolve the Chinese medicine fermentation granules in distilled water to obtain solution A;

[0124] T2: Mix sucrose and solution A evenly, cool, and spray-dry to obtain granules A;

[0125] T3: Divide the pellets A into smaller packages, seal them tightly, and store them at room temperature.

[0126] Composition A:

[0127] The difference from the preparation method of composition 1 is that one distillation is used in step T1, and the remaining steps are the same as those of composition 1.

[0128] Composition B:

[0129] The difference from the preparation method of composition 1 is that in step T1, 93v / v% ethanol reflux extraction is used instead of steam distillation, the solid-liquid ratio is 1:20, the ethanol reflux extraction temperature is 65°C, the reflux times are 2 times, and after reflux, the secondary reflux liquid is diluted with ultrapure water to a concentration of 0.3 mg of hydrocyanic acid per 1 mL, and sealed for later use; in step T2, 93v / v% ethanol reflux extraction is used instead of steam distillation, the solid-liquid ratio is 1:20, the ethanol reflux extraction temperature is 65°C, and the reflux times are 1 time; steps T1-T2 are all at normal pressure; and the remaining steps are the same as composition 1.

[0130] Test 1: Cough Suppression Test

[0131] Test materials: Ammonia water (Guangdong Chemical Reagent Engineering Research and Development Center), codeine phosphate (Qinghai Pharmaceutical Co., Ltd.).

[0132] Experimental animals: KM mice (half male and half female), weighing 24±4 g, SPF grade; purchased from Guangdong Weitonglihua Experimental Animal Technology Co., Ltd.

[0133] Test instrument: ultrasonic atomizer (purchased from Yuehua WH-2000).

[0134] Experimental methods:

[0135] (1) Animal screening

[0136] After the thermal ultrasonic atomizer, add the 40mL ammonia solution (12.5wt%) of fresh preparation. It is 1000mL that mice are put into the beaker of volume, the beaker mouth is airtight with preservative film, and opens two mouths (one for inserting ultrasonic atomizer outlet pipe, the other is ventilation hole). After ultrasonic atomizer is continuously passed into ammonia for 60s with minimum spray intensity, immediately close and open preservative film rapidly so that ammonia is naturally diffused. When passing into ammonia, use stopwatch to time, record mouse cough incubation period (s) and the number of coughs (times) in three minutes. The manifestation of cough is: when opening mouth wide or opening mouth slightly, with coughing sound, all visible abdominal muscle contraction.

[0137] Elimination criteria for mice with cough sensitivity: ① Coughing less than 10 times and more than 80 times within three minutes; ② Latency after spraying less than 15 seconds and more than 50 seconds; ③ Others: animals died, in poor condition, or had abnormally strong reactions; animals that met any of the three criteria were eliminated and not included in subsequent experiments.

[0138] (2) Effect of the composition on the mouse cough model induced by concentrated ammonia

[0139] 210 mice were screened from 240 mice. The qualified animals were randomly divided according to body weight into a control group, a positive group (codeine phosphate; dosage: 15 mg / kg), and a test group (compositions 1-5, compositions ①-⑫, composition A, and composition B; dosage: 35 mg / kg). Each group consisted of 10 mice. The drug-treated groups (including the positive and test groups) were gavaged with the corresponding drug solution (20 mL / kg, solvent: normal saline), while the control group was gavaged with normal saline (20 mL / kg). The drugs were administered three times daily. One hour after the last dose, the mice were challenged with 12.5 wt% ammonia water. The cough latency (s) and the number of coughs within three minutes (tick) were recorded. Statistical Methods: Data are expressed as mean ± standard deviation (x̄ ± s). Statistical analysis was performed using SPSS 17.0 software. One-way analysis of variance was used for statistical analysis. P < 0.05 was considered statistically significant.

[0140] Test results: After three doses, compositions 1-5 all tended to prolong the cough latency period, but there was a significant difference compared with the control group; compositions 1-5 and the positive group (codeine phosphate) significantly reduced the number of coughs within 3 minutes, which was significantly different from the control group.

[0141] Table 2 Effects of three doses of drug administration on the latency period (s) and the number of coughs within three minutes (times) of mice induced by ammonia (x̄±S)

[0142] experimental group Dosage (mg / kg) N Weight (g) Cough incubation period (s) 3min (number of coughs) Death control group - 10 25.36±1.04 42.76±24.52 42.57±5.83 3 Positive group 15 10 24.42±3.52 93.51±19.36** 7.24±3.62** 1 Composition 1 35 10 25.37±6.62 87.42±20.42* 7.6±2.97* 0 Composition 2 35 10 26.12±4.37 82.57±35.73* 8.5±2.15* 0 Composition 3 35 10 24.51±2.54 83.61±30.27* 10.33±4.42* 1 Composition 4 35 10 25.07±4.26 84.36±29.36* 9.7±3.51* 0 Composition 5 35 10 25.36±3.66 81.47±24.51* 8.9±4.27* 0 Composition ① 35 10 25.32±1.08 72.41±19.74* 27.56±10.22* 1 Composition ② 35 10 25.41±4.45 77.52±23.62* 25.37±9.67* 2 Composition ③ 35 10 24.47±3.51 73.14±28.41* 30.22±3.69* 1 Composition ④ 35 10 24.53±4.15 78.36±34.21* 24.21±5.24* 0 Composition ⑤ 35 10 25.14±3.32 61.36±24.53* 30.44±11.36* 1 Composition ⑥ 35 10 24.48±1.39 59.47±27.55* 36.33±9.41* 1 Composition ⑦ 35 10 24.26±2.59 69.42±27.55* 34.33±9.41* 1 Composition ⑧ 35 10 25.33±2.63 65.31±24.36* 36.125±5.82* 2 Composition ⑨ 35 10 24.14±4.21 69.24±35.14* 31.22±6.27* 1 Composition ⑩ 35 10 24.72±1.76 54.72±27.33* 37.33±10.42* 1 Composition ⑪ 35 10 24.58±1.57 54.27±26.42* 35.44±9.93* 1 Composition ⑫ 35 10 24.36±2.41 72.36±21.51* 28.3±6.82* 0 Composition A 35 10 24.41±3.09 76.37±36.71* 24.56±14.27* 1 Composition B 35 10 24.57±2.62 73.26±34.22* 26.67±9.42* 1

[0143] Note: Compared with the control group, *P<0.05, **P<0.01.

[0144] Conclusion: In the animal model, compositions 1-5 (35 mg / kg) all had significant antitussive effects, and composition 1 had an effect similar to that of codeine phosphate.

[0145] Experiment 2: Antiasthmatic effect test

[0146] Test reagents: histamine phosphate, aminophylline solution.

[0147] Experimental animals: 147 SPF guinea pigs (74 females and 73 males), weighing 450±50 g, were purchased from Guangdong Weitonglihua Experimental Animal Technology Co., Ltd.

[0148] Main instruments: Ultrasonic nebulizer (purchased from Yuehua WH-2000).

[0149] Experimental Methods: Guinea pigs were randomly divided according to body weight into a control group, a positive group (aminophylline solution, concentration: 12.5wt%), and a test group (Compositions 1-5, Compositions ①-⑫, Composition A, and Composition B, dosed at 35mg / kg), with seven pigs in each group. The drug-treated groups (including the positive and test groups) were gavaged with the corresponding drug solution (20mL / kg, solvent: normal saline), while the control group was gavaged with normal saline (20mL / kg). Drug administration was performed once daily for three consecutive days. One hour after the last dose, the guinea pigs inhaled 0.4v / v% histamine phosphate. The time from the onset of convulsions or falls was recorded as the latency period (time from the start of spraying to the onset of fall). The pigs were observed for 6 minutes, with those who did not fall within 6 minutes being counted as 360s.

[0150] Statistical methods: Data were expressed as mean ± standard deviation (x̄ ± s). SPSS 17.0 statistical analysis software was used for statistical analysis. One-way analysis of variance was used for analysis and statistics. P < 0.05 was considered statistically significant.

[0151] Test results: After three days of administration, the latent period of asthma onset in compositions 1-5 and the positive group (aminophylline solution) was significantly longer than that in the control group and compositions ①-⑫, with significant differences compared with the control group.

[0152] Table 3 Effects of 3-day drug administration on the latency period (s) of histamine-induced asthma in mice (x̄±S)

[0153] experimental group N Weight (g) Dosage (mg / kg) Convulsion-fall latency (s) control group 7 458.42±15.36 0 47.68±6.42 Positive group 7 476.21±14.28 35 104.32±4.37** Composition 1 7 482.53±25.37 35 99.42±5.46* Composition 2 7 476.12±24.62 35 89.37±6.82* Composition 3 7 468.21±19.27 35 93.52±9.24* Composition 4 7 474.36±14.61 35 95.17±9.73* Composition 5 7 469.32±13.74 35 90.26±8.17* Composition ① 7 465.61±12.98 35 73.51±14.53* Composition ② 7 474.36±12.85 35 75.21±18.42* Composition ③ 7 458.37±12.94 35 74.32±25.47* Composition ④ 7 436.21±15.43 35 72.32±22.76* Composition ⑤ 7 459.24±17.52 35 74.62±24.17* Composition ⑥ 7 458.44±15.12 35 68.73±16.27* Composition ⑦ 7 462.73±16.74 35 69.31±24.66* Composition ⑧ 7 467.22±15.13 35 72.14±21.52* Composition ⑨ 7 455.18±14.52 35 73.61±23.54* Composition ⑩ 7 472.33±13.66 35 55.27±22.76* Composition ⑪ 7 465.27±12.51 35 53.27±35.42* Composition ⑫ 7 471.32±11.36 35 59.61±30.28* Composition A 7 468.25±18.42 35 78.42±29.34* Composition B 7 461.38±12.54 35 76.51±35.44*

[0154] Note: Compared with the control group, *P<0.05, **P<0.01.

[0155] Conclusion: In the experimental animal model, compositions 1-5 (dosage: 35 mg / kg) all had significant antiasthmatic effects, and composition 1 had a similar effect to aminophylline.

[0156] Test 3: Hepatotoxicity and renal toxicity test

[0157] Test reagent: urethane.

[0158] Test animals: 60 KM mice (male), body weight 18±3 g, SPF level, purchased from: Guangdong Weitong Lihua Experimental Animal Technology Co., Ltd.

[0159] Main instrument: optical microscope.

[0160] Test method: randomly divide the mice according to body weight into control group and test group (compositions 1-5; dosage: 35 mg / kg), 10 in each group. The administration groups (including positive group and test group) are given corresponding liquid medicine (20 mL / kg, solvent is normal saline) by gavage, the control group is given normal saline (20 mL / kg) by gavage, continuously for 7 days. Before administration, weigh and record, fast for 12 h before sacrifice, weigh, 15 wt% urethane intraperitoneal injection anesthesia, abdominal aortic blood sampling, serum separation, determination of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea nitrogen (BUN), creatinine (Cr) four biochemical indexes of each group of mice. Sacrifice the mice, take the liver and kidney, respectively, to determine the liver coefficient, kidney coefficient, liver coefficient = liver mass / body quality × 100%, kidney coefficient = kidney mass / body quality × 100%, take the liver and kidney tissues, respectively, with 4 v / v% formaldehyde solution fixation, conventional dehydration, paraffin embedding, section, HE staining, light microscope observation of liver and kidney tissue microstructure.

[0161] Statistical method: data are expressed as mean ± standard deviation (x̄±s), statistical analysis using SPSS17.0 statistical analysis software, using single factor analysis of variance for statistical analysis, P<0.05 is statistically different.

[0162] Table 4 Effect of composition on liver and kidney coefficient of mice (x̄±S)

[0163] experimental group N Weight (g) ALT (IU / L) AST (IU / L) BUN (c / μmol / mL) Cr (c / μmol / mL) Liver coefficient Kidney coefficient control group 10 17.36±0.24 18.6±1.75 15.7±1.35 3.1±1.4 65.4±10.37 6.543±0.427 1.643±0.144 Composition 1 10 17.51±1.04 19.4±2.63 15.8±1.17 3.3±1.2 68.2±9.82 6.633±0.624 1.674±0.182 Composition 2 10 17.14±0.98 20.3±2.51 15.6±1.62 3.5±1.1 67.4±10.34 6.637±0.374 1.694±0.193 Composition 3 10 17.39±1.44 19.3±1.72 15.9±1.83 3.4±1.2 69.3±11.57 6.649±0.455 1.702±0.215 Composition 4 10 17.08±1.07 19.6±1.67 16.1±1.75 3.3±1.1 68.7±10.93 6.638±0.362 1.698±0.248 Composition 5 10 17.94±0.95 19.1±2.14 15.7±1.42 3.2±1.4 68.9±11.51 6.721±0.541 1.731±0.174

[0164] Note: compared with the control group, *P<0.05.

[0165] Conclusion: the pathological section observation of compositions 1-5 is basically similar to the control group, no obvious degeneration, necrosis and pathological abnormalities of liver and kidney cells are found, and each index is basically similar to the control group, without obvious difference.

[0166] The above-described embodiments are part of the embodiments of the present application, rather than all the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the claimed application, but only represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

Claims

1. A cough and asthma relieving composition, characterized in that: The antitussive and antiasthmatic composition is made from the following components in parts by mass: 5.4-5.6 parts of Chinese herbal fermented granules; 1-1.3 parts bitter almonds; 1-1.3 parts of perilla leaves; Peucedanum 1-1.3 parts; 90-92 parts of sucrose; The specific steps of the preparation method of the traditional Chinese medicine fermented granules are as follows: S1: Grind tangerine peel, platycodon grandiflorum, and liquorice together, and pass through a 400-600 mesh sieve to obtain a mixed powder, wherein the mass ratio of tangerine peel, platycodon grandiflorum, and liquorice is 1:1:0.3-0.4; S2: Add sterile deionized water 10 times the mass of the mixed powder to the mixed powder and stir evenly to obtain a mixed solution; S3: adding cellulase to the mixed solution for enzymolysis, wherein the amount of cellulase added is 5-7 wt % of the mixed solution, the enzymolysis temperature is 30-35° C., and the enzymolysis time is 3-6 h. After the enzymolysis is completed, filtering is performed to obtain an enzymolysis solution; S4: adding 9-12 wt% glucose and 3 wt% peptone to the enzymatic hydrolysate, mixing well, sterilizing the enzymatic hydrolysate at 105°C for 40 min, and filtering to obtain a filtrate; S5: inoculating Lactobacillus plantarum, Lactobacillus johnsonii, and Rhodotorula glutinosae into the filtrate and performing mixed fermentation. After the fermentation is completed, the mixture is sterilized at high temperature and filtered to obtain a traditional Chinese medicine fermentation liquid, wherein the mass ratio of Lactobacillus plantarum, Lactobacillus johnsonii, and Rhodotorula glutinosae is 1:0.5-1:1, the total amount of bacteria is 8-10wt% of the filtrate, the fermentation time is 12-16h, the fermentation temperature is 28-30°C, the fermentation pH is 5.8-7.1, and the sterile air ventilation rate is 3L / min; the number of the Lactobacillus johnsonii is CGMCC 1.10778, the number of the Lactobacillus plantarum is CGMCC 1.568, and the number of the Rhodotorula glutinosae is CGMCC 2.5690; S6: Concentrating the Chinese medicine fermentation liquid, spray drying and granulating to obtain Chinese medicine fermentation granules.

2. The antitussive and antiasthmatic composition according to claim 1, characterized in that The antitussive and antiasthmatic composition is made from the following components in parts by mass: 5.4-5.5 parts of Chinese herbal fermented granules; 1-1.15 parts bitter almonds; 1-1.15 parts of perilla leaves; Peucedanum 1-1.15 parts; Sucrose 91-92 parts.

3. The antitussive and antiasthmatic composition according to claim 1, characterized in that The antitussive and antiasthmatic composition is made from the following components in parts by mass: 5.5 parts of Chinese herbal fermented granules; 1.15 parts bitter almonds; 1.15 parts of perilla leaves; Peucedanum 1.15 parts; 91.05 parts of sucrose.

4. A method for preparing the cough and asthma relieving composition according to any one of claims 1 to 3, characterized in that: The following steps are included: T1: Crush bitter almonds, soak in water for 20-26 hours, steam distill, collect the distillate, add 1-2 v / v% 90-95 v / v% ethanol to the distillate, distill again, collect the secondary distillate, dilute the secondary distillate with ultrapure water to a concentration of 0.3 mg of hydrocyanic acid per 1 mL, and seal for later use; T2: Extract the perilla leaves and peucedanum chinense by steam distillation and seal them for later use; T3: dissolving the Chinese medicinal fermentation granules in the bitter almond double distilled dilution solution to obtain a mixed solution; T4: Evenly mix the perilla leaf extract, peucedanum peucedanum extract, sucrose, and the mixed solution obtained in step T3, cool, and spray-dry to obtain finished granules; T5: Divide the finished granules into smaller pieces, seal them tightly, and store them at room temperature.