Method for using nitrogen as treatment gas for experimental animal euthanasia

Abstract

A method for using nitrogen as treatment gas for experimental animal euthanasia comprises the steps that firstly, air enters a small compressor to be compressed, the compressed air enters a three-position five-way electromagnetic valve through an air inlet pipe, high-pressure oxygen is adsorbed by a carbon molecular sieve in a left reaction tank, the remaining nitrogen is collected, and after nitrogen collection is completed, nitrogen in the left reaction tank is discharged out of the left reaction tank; a left air outlet of the three-position five-way electromagnetic valve is opened, at the moment, the pressure in the left reaction tank is reduced, oxygen adsorbed on the carbon molecular sieve is desorbed, air remaining in the left reaction tank is discharged together, meanwhile, compressed air enters the right reaction tank through the three-position five-way electromagnetic valve and a right circulating pipe for reaction, and the reaction process is the same as the process in the left reaction tank; and sequentially carrying out reciprocating circular reaction. Nitrogen can be obtained more conveniently, the cost is low, it can be ensured that animals do not experience any anxiety, discomfort or pain in the death process by using nitrogen as euthanasia gas, and the humanity and efficiency of animal euthanasia are improved.

Description

Technical Field

[0001] The invention relates to an improvement of an experimental animal euthanasia technology, belongs to the field of experimental animals, and in particular to a method of using nitrogen as a processing gas for euthanasia of experimental animals. Background Art

[0002] Laboratory animal euthanasia refers to a humane treatment of laboratory animals when a scientific experiment is over or needs to be terminated to avoid unnecessary pain and suffering. It is usually carried out to terminate the experiment, end the animal's pain or achieve the purpose of research. This treatment method usually uses specific methods or drugs to quickly and painlessly end the life of the laboratory animal. The purpose of euthanasia is to ensure that the laboratory animal is respected and treated decently at the last moment, while minimizing the pain and anxiety of the laboratory animal. In general, laboratory animal euthanasia is an important measure to protect the welfare of laboratory animals and reduce their pain, while ensuring the compliance and accuracy of scientific research. When conducting laboratory animal euthanasia, strict laws, regulations and ethical standards must be followed, and appropriate methods and measures must be taken to ensure that the animals do not suffer pain. At present, euthanasia mostly uses carbon dioxide asphyxiation, and carbon dioxide is inconvenient to obtain: it needs to be purchased from specific merchants, the price is expensive, and there are some purchase cycles, and it cannot be used anytime and anywhere.

[0003] A Chinese patent application with application number CN201911075874.2 and application date November 6, 2019 discloses a large experimental animal euthanasia system, which relates to the field of experimental animal technology and is used to solve the problem that animals die painfully when euthanized by carbon dioxide euthanasia. The invention comprises a shell, a partition is provided in the shell, the partition divides the shell into a cabin and an electrical control box, an air extraction port is provided on the partition, a vacuum pump is provided in the electrical control box, the vacuum pump is connected to the air extraction port through an air outlet pipe, a regulating valve is provided on the air inlet pipe, an exhaust port is provided on the side of the electrical control box, the front end of the cabin is open and provided with a closed door, and a refrigeration system for cooling the cabin is provided in the shell; the invention has the advantages of simple structure, saving the cost and time of euthanasia of experimental animals, and being more in line with the regulations on the management of experimental animals and animal welfare, but the above technology does not solve the problem of inconvenient acquisition of carbon dioxide for euthanasia.

[0004] The information disclosed in this background technology section is only intended to increase the understanding of the overall background of this patent application, and should not be regarded as acknowledging or suggesting in any form that the information constitutes the prior art already known to ordinary technicians in this field. Summary of the invention

[0005] The purpose of the present invention is to overcome the problem of inconvenient acquisition of carbon dioxide for euthanasia in the prior art, and to provide a method for conveniently acquiring carbon dioxide for euthanasia using nitrogen as a treatment gas for euthanasia of experimental animals.

[0006] To achieve the above objectives, the technical solution of the present invention is: a method of using nitrogen as a processing gas for euthanasia of experimental animals. The method of using nitrogen as a processing gas for euthanasia of experimental animals is as follows: First, the air enters the small compressor for compression, and the compressed air enters the three-position five-way solenoid valve through the air intake pipe. The three-position five-way solenoid valve is connected to the left reaction tank through the left circulation pipe, and is connected to the right reaction tank through the right circulation pipe. When the compressed air enters the left reaction tank through the left circulation pipe, the high-pressure oxygen is adsorbed by the carbon molecular sieve in the left reaction tank, and the remaining nitrogen is collected. When the nitrogen is collected, the left air outlet of the three-position five-way solenoid valve is opened. At this time, the pressure in the left reaction tank is reduced, and the oxygen adsorbed on the carbon molecular sieve is desorbed, and the air remaining in the left reaction tank is discharged together. At the same time, the compressed air enters the right reaction tank through the three-position five-way solenoid valve and the right circulation pipe for reaction. The reaction process is the same as that in the left reaction tank, and the reaction is repeated in sequence. When enough nitrogen is collected, the left reaction tank and the right reaction tank are closed, and then the nitrogen is transported to the area where the experimental animals are to be treated.

[0007] The three-position five-way solenoid valve comprises a housing, a left circulation port, a right circulation port, an air inlet, a left air outlet and a right air outlet, wherein the left circulation port is connected to the left circulation pipe, the right circulation port is connected to the right circulation pipe, and the air inlet is connected to the air inlet pipe; A slide groove is provided inside the housing, a valve core is slidably fitted in the slide groove, and a left spring ring and a right spring ring are connected to the left and right ends of the valve core respectively; A left coil shell is installed on the left side of the shell, a left electromagnetic coil is arranged in the left coil shell, and the left electromagnetic coil is magnetically connected to the left spring ring. A right coil shell is installed on the right side of the shell, a right electromagnetic coil is arranged in the right coil shell, and the right electromagnetic coil is magnetically connected to the right spring ring.

[0008] The valve core includes one valve core column, two valve core columns, three valve core columns and a valve shaft. The one valve core column, the two valve core columns and the three valve core columns are connected through the valve shaft, and the one valve core column, the two valve core columns and the three valve core columns are all slidably connected to the slide groove.

[0009] The left side of the valve core column is connected to the right side of the left connecting end, and the left side of the left connecting end is connected to the right side of the left spring ring; The right end of the three-valve core column is connected to the left side of the right connecting end, and the right side of the right connecting end is connected to the left side of the right spring ring.

[0010] The diameters of the first valve core column, the second valve core column, and the third valve core column are the same, the diameters of the left connecting end and the right connecting end are the same, the diameter of the first valve core column is larger than the diameter of the left connecting end, the left connecting end is larger than the diameter of the valve shaft, and a gas flow area exists between the valve shaft and the slide groove.

[0011] A left empty slot and a right empty slot are respectively provided on the left and right sides of the shell, the left spring ring is arranged in the left empty slot, and a left connecting plate is arranged between the left spring ring and the left connecting end; The right spring ring is arranged in the right empty groove, and a right connecting plate is arranged between the right spring ring and the right connecting end.

[0012] The air inlet of the three-position five-way solenoid valve is threadedly connected with a filtering mechanism.

[0013] The filtering mechanism includes a top ring, a filter ring and a bottom ring. The outer periphery of the top ring is threadedly connected to the air inlet, the inner periphery of the top ring is provided with a filter screen, the bottom of the top ring is connected to the top of the filter ring, the bottom of the filter ring is connected to the top of the bottom ring, and a plurality of filter holes are provided on the side of the filter ring.

[0014] An inlet is arranged at the center of the bottom ring; The diameter of the top ring decreases along the filter ring to the bottom ring; The diameter of the inlet is larger than the diameter of the filter hole, and the diameter of the filter hole is larger than the aperture of the filter screen.

[0015] A plurality of filter holes are evenly distributed in a ring shape on the side of the filter ring, and the filter ring is arranged in an arc shape.

[0016] Compared with the prior art, the present invention has the following beneficial effects: 1. In a method of using nitrogen as a treatment gas for euthanasia of experimental animals, the method uses nitrogen extracted from the air, which is easier to obtain, has a wide range of sources, and has a low cost. At the same time, existing nitrogen generation devices all pursue high purity, while the nitrogen used in the present invention does not require a very high purity, because the ammonia extracted by the present invention is used for euthanasia, and the dead object is the rodent after the experiment. Compared with the traditional method, the use of nitrogen as the euthanasia gas can ensure that the animal will not experience any anxiety, discomfort or pain during the death process, thereby improving the humanity and efficiency of animal euthanasia. Therefore, the nitrogen of the present invention is more convenient to obtain and has a low cost.

[0017] 2. In the method of using nitrogen as a treatment gas for euthanasia of experimental animals of the present invention, the preparation of nitrogen by this method is a cyclic process, which can be prepared cyclically, and the nitrogen can be recovered and reused after being used in the process of euthanasia of experimental animals. The recovery and reuse of nitrogen can also reduce the operating cost of the laboratory, improve the efficiency of resource utilization, reduce the impact on the environment, and realize the sustainable utilization of resources. Therefore, the present invention improves the utilization rate of resources and reduces energy consumption.

[0018] 3. In a method of using nitrogen as a processing gas for euthanasia of experimental animals, the air inlet of the three-position five-way solenoid valve is threadedly connected with a filter mechanism, and the filter mechanism includes a top ring, a filter ring and a bottom ring. The outer periphery of the top ring is threadedly connected to the air inlet, and the inner periphery of the top ring is provided with a filter screen. The bottom of the top ring is connected to the top of the filter ring, and the bottom of the filter ring is connected to the top of the bottom ring. The side of the filter ring is provided with a plurality of filter holes. When used, when the compressed air enters the housing from the air inlet, impurities in the gas will be blocked in the area between the filter ring and the bottom ring, and the filter ring is made of a certain magnetic material, which can adsorb part of the particles, and the filter screen on the side of the filter ring can improve the efficiency of air entry, and the design of the arc belt with a certain length can prolong the time for air to pass, so that the adsorption effect is better, thereby avoiding impurities or particles from entering the three-position five-way solenoid valve to affect the use, and improving the safety of use. Therefore, the present invention can adsorb particulate impurities and is safer to use. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a structural schematic diagram of the present invention.

[0020] Figure 2 It is a structural schematic diagram of a three-position five-way solenoid valve in the present invention.

[0021] Figure 3 It is a structural schematic diagram of the valve core in the present invention.

[0022] Figure 4 It is a structural schematic diagram of the filtering mechanism in the present invention.

[0023] Figure 5 It is a bottom view of the filtering mechanism in the present invention.

[0024] In the figure: small compressor 1, air intake pipe 2, three-position five-way solenoid valve 3, shell 31, left circulation port 32, right circulation port 33, left air outlet 34, air intake port 35, right air outlet 36, slide slot 37, valve core 38, right connecting end 381, first valve core column 382, ​​valve shaft 383, second valve core column 384, third valve core column 385, right connecting end 386, gas circulation area 39, left coil shell 310, left electromagnetic coil 311, left connecting plate 312, left spring ring 313, right coil shell 314, right electromagnetic coil 315, right spring ring 316, right connecting plate 317, left empty slot 318, right empty slot 319, left circulation pipe 4, right circulation pipe 41, left reaction tank 5, right reaction tank 51, filtering mechanism 6, top ring 61, filter screen 62, filter ring 63, filter hole 64, bottom ring 65, inlet 66. DETAILED DESCRIPTION

[0025] The present invention is further described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0026] See also Figures 1 to 5 A method for using nitrogen as a processing gas for euthanasia of experimental animals. The method for using nitrogen as a processing gas for euthanasia of experimental animals is as follows: First, air enters the small compressor 1 for compression, and the compressed air enters the three-position five-way solenoid valve 3 through the air intake pipe 2. The three-position five-way solenoid valve 3 is connected to the left reaction tank 5 through the left circulation pipe 4, and is connected to the right reaction tank 51 through the right circulation pipe 41. When the compressed air enters the left reaction tank 5 through the left circulation pipe 4, the high-pressure oxygen is adsorbed by the carbon molecular sieve in the left reaction tank 5, and the remaining nitrogen is collected. When the nitrogen is collected, the left air outlet 34 of the three-position five-way solenoid valve 3 is opened. At this time, the pressure in the left reaction tank 5 is reduced, and the oxygen adsorbed on the carbon molecular sieve is desorbed, and the air remaining in the left reaction tank 5 is discharged together. At the same time, the compressed air enters the right reaction tank 51 through the three-position five-way solenoid valve 3 and the right circulation pipe 41 to react. The reaction process is the same as that in the left reaction tank 5, and the reaction is repeated in sequence. When a sufficient amount of nitrogen is collected, the left reaction tank 5 and the right reaction tank 51 are closed, and then the nitrogen is transported to the area where the experimental animals are to be treated.

[0027] The three-position five-way solenoid valve 3 includes a housing 31, a left circulation port 32, a right circulation port 33, an air inlet 35, a left air outlet 34 and a right air outlet 36. The left circulation port 32 is connected to the left circulation pipe 4, the right circulation port 33 is connected to the right circulation pipe 41, and the air inlet 35 is connected to the air inlet pipe 2; The housing 1 has a slide groove 37 formed inside, a valve core 38 is slidably fitted in the slide groove 37, and the left and right ends of the valve core 38 are respectively connected to a left spring ring 313 and a right spring ring 316; A left coil housing 310 is installed on the left side of the shell 1, and a left electromagnetic coil 311 is arranged in the left coil housing 310. The left electromagnetic coil 311 is magnetically connected to the left spring ring 313. A right coil housing 314 is installed on the right side of the shell 1. A right electromagnetic coil 315 is arranged in the right coil housing 314. The right electromagnetic coil 315 is magnetically connected to the right spring ring 316.

[0028] The valve core 38 includes a valve core column 382, ​​a second valve core column 384, a third valve core column 385 and a valve shaft 383. The valve core column 382, ​​the second valve core column 384, and the third valve core column 385 are connected by the valve shaft 383, and the valve core column 382, ​​the second valve core column 384, and the third valve core column 385 are all slidably connected to the slide groove 37.

[0029] The left side of the valve core column 382 is connected to the right side of the left connecting end 381, and the left side of the left connecting end 381 is connected to the right side of the left spring ring 313; The right end of the three-valve core column 385 is connected to the left side of the right connecting end 386 , and the right side of the right connecting end 381 is connected to the left side of the right spring coil 316 .

[0030] The diameters of the first valve core column 382, ​​the second valve core column 384, and the third valve core column 385 are the same, the diameters of the left connecting end 381 and the right connecting end 386 are the same, the diameter of the first valve core column 382 is larger than the diameter of the left connecting end 381, the left connecting end 381 is larger than the diameter of the valve shaft 383, and there is a gas circulation area 39 between the valve shaft 383 and the slide groove 37.

[0031] The left and right sides of the housing 31 are respectively provided with a left slot 318 and a right slot 319, the left spring ring 313 is arranged in the left slot 318, and a left connecting plate 312 is arranged between the left spring ring 313 and the left connecting end 381; The right spring ring 316 is arranged in the right empty groove 319 , and a right connecting plate 317 is arranged between the right spring ring 316 and the right connecting end 386 .

[0032] The air inlet 35 of the three-position five-way solenoid valve 3 is threadedly connected with a filter mechanism 6 .

[0033] The filtering mechanism 6 includes a top ring 61, a filter ring 63 and a bottom ring 65. The outer periphery of the top ring 61 is threadedly connected to the air inlet 35, the inner periphery of the top ring 61 is provided with a filter screen 62, the bottom of the top ring 61 is connected to the top of the filter ring 63, the bottom of the filter ring 63 is connected to the top of the bottom ring 65, and a plurality of filter holes 64 are provided on the side of the filter ring 63.

[0034] An inlet 66 is provided at the center of the bottom ring 65; The diameter of the top ring 61 decreases along the filter ring 63 to the bottom ring 65; The diameter of the inlet 66 is larger than the diameter of the filter hole 64 , and the diameter of the filter hole 64 is larger than the aperture of the filter screen 62 .

[0035] A plurality of filter holes 64 are evenly distributed in a ring shape on the side of the filter ring 63, and the filter ring 63 is arranged in an arc shape.

[0036] Embodiment 1: A method for using nitrogen as a processing gas for euthanasia of experimental animals The three-position five-way solenoid valve 3 includes a shell 31, a left circulation port 32, a right circulation port 33, an air inlet 35, a left air outlet 34 and a right air outlet 36. The left circulation port 32 is connected to the left circulation pipe 4, the right circulation port 33 is connected to the right circulation pipe 41, and the air inlet 35 is connected to the air inlet pipe 2; a slide groove 37 is provided inside the shell 1, a valve core 38 is slidably fitted in the slide groove 37, and the left and right ends of the valve core 38 are respectively connected to The left spring ring 313 and the right spring ring 316; the left coil housing 310 is installed on the left side of the housing 1, and the left electromagnetic coil 311 is arranged in the left coil housing 310, and the left electromagnetic coil 311 is magnetically connected to the left spring ring 313; the right coil housing 314 is installed on the right side of the housing 1, and the right electromagnetic coil 315 is arranged in the right coil housing 314, and the right electromagnetic coil 315 is magnetically connected to the right spring ring 316; the valve core 38 includes a valve core column 382, ​​a second valve core column 384, and a third valve core column 385. The valve core column 385 is connected to the valve shaft 383, and the first valve core column 382, ​​the second valve core column 384, and the third valve core column 385 are connected through the valve shaft 383. The first valve core column 382, ​​the second valve core column 384, and the third valve core column 385 are all slidably connected to the slide groove 37; the left side of the first valve core column 382 is connected to the right side of the left connecting end 381, and the left side of the left connecting end 381 is connected to the right side of the left spring ring 313; the right end of the third valve core column 385 is connected to the left side of the right connecting end 386, and the right connecting end 381 The right side is connected to the left side of the right spring ring 316; the diameters of the first valve core column 382, ​​the second valve core column 384, and the third valve core column 385 are the same, the diameters of the left connecting end 381 and the right connecting end 386 are the same, the diameter of the first valve core column 382 is larger than the diameter of the left connecting end 381, the left connecting end 381 is larger than the diameter of the valve shaft 383, and there is a gas flow area 39 between the valve shaft 383 and the slide groove 37; the left and right sides of the shell 31 are respectively provided with a left empty groove 318 and a right empty groove 319, the left spring ring 313 is arranged in the left empty groove 318, and a left connecting plate 312 is arranged between the left spring ring 313 and the left connecting end 381; the right spring ring 316 is arranged in the right empty groove 319, and a right connecting plate 317 is arranged between the right spring ring 316 and the right connecting end 386.

[0037] When applying: First, air enters the small compressor 1 for compression. The compressed air enters the three-position five-way solenoid valve 3 through the intake pipe 2. The three-position five-way solenoid valve 3 is connected with the left reaction tank 5 through the left circulation pipe 4, and is connected with the right reaction tank 51 through the right circulation pipe 41. The left electromagnetic coil 311 is energized, and the right electromagnetic coil 315 is not energized. At this time, the valve core 38 moves to the right, the left spring coil 313 is stretched, and the right spring coil 316 is compressed. The channel between the left circulation port 32 and the air inlet 35 is opened, and the compressed air flows into the left circulation pipe 4 after passing through the air inlet 35, the gas flow area 39, and the left circulation port 32 in turn. When the compressed air enters the left reaction tank 5, the high-pressure oxygen is The carbon molecular sieve in the reaction tank 5 is adsorbed and the remaining nitrogen is collected. When the nitrogen is collected, the right electromagnetic coil 315 is energized and the left electromagnetic coil 311 is not energized. At this time, the valve core 38 moves to the left, the left spring coil 313 is compressed, and the right spring coil 316 is stretched. At this time, the pressure in the left reaction tank 5 is reduced, and the oxygen adsorbed on the carbon molecular sieve is desorbed, and the gas remaining in the left reaction tank 5 is discharged through the left air outlet 34. At the same time, the compressed air enters the right reaction tank 51 through the air inlet 35, the gas flow area 39, and the right circulation port 33 through the right circulation pipe 41 to react. The reaction process is the same as the process in the left reaction tank 5, and the reaction is repeated in sequence until an appropriate amount of nitrogen is collected.

[0038] Embodiment 2: Embodiment 2 is substantially the same as Embodiment 1, except that: A method for using nitrogen as a processing gas for euthanasia of experimental animals. The air inlet 35 of the three-position five-way solenoid valve 3 is threadedly connected with a filter mechanism 6; the filter mechanism 6 includes a top ring 61, a filter ring 63 and a bottom ring 65, the outer periphery of the top ring 61 is threadedly connected to the air inlet 35, the inner periphery of the top ring 61 is provided with a filter screen 62, the bottom of the top ring 61 is connected to the top of the filter ring 63, the bottom of the filter ring 63 is connected to the top of the bottom ring 65, and a plurality of filter holes 64 are provided on the side of the filter ring 63; an inlet 66 is provided at the center of the bottom ring 65; the diameter of the top ring 61 along the filter ring 63 to the bottom ring 65 is in a decreasing trend; the diameter of the inlet 66 is larger than the diameter of the filter hole 64, and the diameter of the filter hole 64 is larger than the aperture of the filter screen 62; a plurality of filter holes 64 are evenly distributed in a ring shape on the side periphery of the filter ring 63, and the filter ring 63 is arranged in an arc shape.

[0039] When applying: When compressed air enters the shell 31 from the air inlet, impurities in the gas will be blocked in the area between the filter ring 63 and the bottom ring 65. The filter ring 63 is made of a certain magnetic material and can adsorb some particles. The filter screen on the side of the filter ring 63 can improve the efficiency of air entry, and the design of the arc belt with a certain length can extend the time for air to pass through, thereby achieving a better adsorption effect.

[0040] The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiment. Any equivalent modifications or changes made by ordinary technicians in this field based on the contents disclosed by the present invention should be included in the protection scope recorded in the claims.

Claims

1. A method for using nitrogen as a treatment gas for euthanasia of experimental animals, characterized in that: The method of using nitrogen as the treatment gas for euthanasia of experimental animals is specifically as follows: First, air enters the small compressor (1) for compression. The compressed air enters the three-position five-way solenoid valve (3) through the air intake pipe (2). The three-position five-way solenoid valve (3) is connected to the left reaction tank (5) through the left circulation pipe (4), and is connected to the right reaction tank (51) through the right circulation pipe (41). When the compressed air enters the left reaction tank (5) through the left circulation pipe (4), the high-pressure oxygen is adsorbed by the carbon molecular sieve in the left reaction tank (5), and the remaining nitrogen is collected. When the nitrogen is collected, the three-position five-way solenoid valve is opened. (3) is connected to the left air outlet (34). At this time, the pressure in the left reaction tank (5) decreases, the oxygen adsorbed on the carbon molecular sieve is desorbed, and the air remaining in the left reaction tank (5) is discharged together. At the same time, the compressed air enters the right reaction tank (51) through the three-position five-way solenoid valve (3) and the right circulation pipe (41) to react. The reaction process is the same as the process in the left reaction tank (5). The reaction is repeated in sequence. When a sufficient amount of nitrogen is collected, the left reaction tank (5) and the right reaction tank (51) are closed, and then the nitrogen is transported to the area where the experimental animals are to be treated.

2. A method for using nitrogen as a processing gas for euthanasia of experimental animals according to claim 1, characterized in that: The three-position five-way solenoid valve (3) comprises a housing (31), a left circulation port (32), a right circulation port (33), an air inlet (35), a left air outlet (34) and a right air outlet (36); the left circulation port (32) is connected to a left circulation pipe (4), the right circulation port (33) is connected to a right circulation pipe (41), and the air inlet (35) is connected to an air inlet pipe (2); The housing (1) has a slide groove (37) formed inside, a valve core (38) slidably fitted in the slide groove (37), and the left and right ends of the valve core (38) are respectively connected to a left spring ring (313) and a right spring ring (316); A left coil housing (310) is installed on the left side of the housing (1), a left electromagnetic coil (311) is arranged inside the left coil housing (310), and the left electromagnetic coil (311) is magnetically connected to the left spring coil (313); a right coil housing (314) is installed on the right side of the housing (1), a right electromagnetic coil (315) is arranged inside the right coil housing (314), and the right electromagnetic coil (315) is magnetically connected to the right spring coil (316).

3. A method for using nitrogen as a processing gas for euthanasia of experimental animals according to claim 2, characterized in that: The valve core (38) comprises a first valve core column (382), a second valve core column (384), a third valve core column (385) and a valve shaft (383); the first valve core column (382), the second valve core column (384) and the third valve core column (385) are connected via the valve shaft (383); the first valve core column (382), the second valve core column (384) and the third valve core column (385) are all slidably connected to the slide groove (37).

4. The method of using nitrogen as a processing gas for euthanasia of experimental animals according to claim 3, characterized in that: The left side of the valve core column (382) is connected to the right side of the left connecting end (381), and the left side of the left connecting end (381) is connected to the right side of the left spring coil (313); The right end of the three-valve core column (385) is connected to the left side of the right connecting end (386), and the right side of the right connecting end (381) is connected to the left side of the right spring coil (316).

5. A method for using nitrogen as a processing gas for euthanasia of experimental animals according to claim 4, characterized in that: The first valve core column (382), the second valve core column (384), and the third valve core column (385) have the same diameter, the left connecting end (381) and the right connecting end (386) have the same diameter, the diameter of the first valve core column (382) is larger than the diameter of the left connecting end (381), the left connecting end (381) is larger than the diameter of the valve shaft (383), and a gas flow area (39) is present between the valve shaft (383) and the slide groove (37).

6. The method of using nitrogen as a processing gas for euthanasia of experimental animals according to claim 4, characterized in that: The left and right sides of the housing (31) are respectively provided with a left empty slot (318) and a right empty slot (319), the left spring ring (313) is arranged in the left empty slot (318), and a left connecting plate (312) is arranged between the left spring ring (313) and the left connecting end (381); The right spring ring (316) is arranged in the right empty groove (319), and a right connecting plate (317) is arranged between the right spring ring (316) and the right connecting end (386).

7. The method of using nitrogen as a processing gas for euthanasia of experimental animals according to claim 2, characterized in that: The air inlet (35) of the three-position five-way solenoid valve (3) is threadedly connected with a filter mechanism (6).

8. The method of using nitrogen as a processing gas for euthanasia of experimental animals according to claim 7, characterized in that: The filtering mechanism (6) comprises a top ring (61), a filter ring (63) and a bottom ring (65); the outer periphery of the top ring (61) is threadedly connected to the air inlet (35); the inner periphery of the top ring (61) is provided with a filter screen (62); the bottom of the top ring (61) is connected to the top of the filter ring (63); the bottom of the filter ring (63) is connected to the top of the bottom ring (65); and a plurality of filtering holes (64) are provided on the side of the filter ring (63).

9. A method for using nitrogen as a processing gas for euthanasia of experimental animals according to claim 8, characterized in that: An inlet (66) is provided at the center of the bottom ring (65); The diameter of the top ring (61) decreases along the filter ring (63) to the bottom ring (65); The diameter of the inlet (66) is greater than the diameter of the filter hole (64), and the diameter of the filter hole (64) is greater than the aperture of the filter screen (62).

10. A method for using nitrogen as a processing gas for euthanasia of experimental animals according to claim 9, characterized in that: The plurality of filter holes (64) are evenly distributed in a ring shape on the side of the filter ring (63), and the filter ring (63) is arranged in an arc shape.

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