Composition for inhibiting oral pathogens, preparation method and application

Abstract

The present invention belongs to the field of oral care technology, and specifically relates to a composition, preparation method and application for inhibiting oral pathogens. The raw materials of the composition are selected from peony bark, cloves and / or licorice. The preparation method comprises extracting the raw materials of the composition with water separately, and mixing the extracts to obtain the composition; or, mixing the raw materials of the composition, extracting with water to obtain the composition. The present invention has found that the combination of peony bark and cloves, and the combination of peony bark and licorice show synergistic effects on Streptococcus mutans and Porphyromonas gingivalis respectively; the combination of cloves, peony bark and licorice has a synergistic inhibitory effect on the oral pathogens Streptococcus mutans and Porphyromonas gingivalis at the same time. The present invention selects eight kinds of traditional Chinese medicines such as licorice, mulberry leaves, honeysuckle and their combination formulas to conduct in vitro antibacterial test research on oral pathogens Streptococcus mutans and Porphyromonas gingivalis, aiming to provide a reference for the research and development of oral health preparations.

Description

Technical Field

[0001] The present invention belongs to the technical field of oral care, and in particular relates to a composition for inhibiting oral pathogens, a preparation method and an application thereof. Background Art

[0002] Dental caries and bad breath are common oral diseases in clinical practice, which seriously affect human health and quality of life.

[0003] Streptococci mutans (SM), a Gram-positive, facultative anaerobic cocci, is the primary pathogen causing dental caries, leading to damage to tooth tissue and structure. Porphyromonas gingivalis (Pg), a Gram-negative anaerobic bacterium, can cause infections such as dental plaque, oral ulcers, and periodontitis, resulting in bad breath.

[0004] Conventional treatment for oral diseases is with antibiotics, but long-term use of antibiotics can easily lead to disturbances in the oral microbiome. Currently, studies have found that tea, propolis, and other foods have inhibitory effects on oral pathogens.

[0005] Chinese invention patent application CN201510242391.2 discloses a traditional Chinese medicine preparation for treating dental caries, the weight parts of which are as follows: 10-15 parts of coptis root, 4-5 parts of apricot kernel, 2-4 parts of cynanchum chinense, 6-8 parts of xanthium sibiricum, 8-10 parts of red cohosh, 10-12 parts of gallnut, 10-15 parts of roasted liquorice root, 4-5 parts of ligusticum chuanxiong, 2-4 parts of magnolia bark, 6-8 parts of radix polygoni multiflori, 4-5 parts of lilac flower, 10-12 parts of wine-rhubarb, 4-5 parts of morinda officinalis, 2-4 parts of sophora flavescens root, 6-8 parts of haiercha, 8-10 parts of moutan bark, 1-3 parts of zedoaria root, 4-5 parts of evodia rutaecarpa, 6-8 parts of zizyphus zymoides, 2-4 parts of oyster, 1-3 parts of yanhusuo root, 3-4 parts of hydrangea flower, and 2-4 parts of radix scutellariae root. ; Through scientific compatibility, the Chinese medicine preparations have the mutual influence and synergistic effect of the medicines to achieve the purpose of treating both the symptoms and the root causes, and treating both the deficiency and the excess. It has the efficacy of clearing away heat and purging fire, killing insects and relieving itching, and attacking toxins and reducing swelling.

[0006] However, the above-mentioned methods use many raw materials, have complex formulas, and the interactions between the raw materials are difficult to explain. The effects also need further research and improvement. Summary of the Invention

[0007] In view of the problems existing in the prior art, the present invention provides a composition for inhibiting oral pathogens, a preparation method and applications.

[0008] In order to achieve the purpose of the invention, the technical solutions adopted are as follows:

[0009] A composition for inhibiting oral pathogens, wherein the raw materials of the composition are selected from peony bark, clove and / or liquorice.

[0010] Preferably, the raw materials of the composition consist of peony bark and cloves in a mass ratio of 1:1-3, or consist of peony bark extract and clove extract in a mass ratio of 1:1-3.

[0011] Preferably, the raw materials of the composition consist of Paeonia suffruticosa and Licorice at a mass ratio of 1-3:1, or consist of Paeonia suffruticosa extract and Licorice extract at a mass ratio of 1-3:1.

[0012] Preferably, the raw materials of the composition are composed of peony bark, cloves and licorice in a mass ratio of 0.25-8:0.25-8:1; preferably, the mass ratio of peony bark: cloves: licorice = 4:1:4, 2:8:1, 8:2:1, 4:0.5:1, 0.25:0.25:1, 4:8:1, 2:0.5:1.

[0013] The most preferred mass ratios are peony bark:clove:licorice=4:1:4, 2:8:1, 8:2:1 or 4:0.5:1.

[0014] The peony bark, cloves, and licorice in the above raw materials can be replaced with peony bark extract, clove extract, and licorice extract. The preparation method of the above peony bark extract, clove extract, or licorice extract comprises extracting the peony bark, clove, or licorice with water. Preferably, the amount of water used is 5-15 times the total mass of the raw materials, and the extraction is performed by decoction, the number of decoctions is 1-3, the decoction time for each decoction is 0.5-2 hours, and the decoction temperature is 95-105°C.

[0015] The second object of the present invention is to provide a method for preparing a composition for inhibiting oral pathogens, comprising extracting the raw materials of the composition with water separately and mixing the extracts to obtain the composition; or mixing the raw materials of the composition and extracting with water to obtain the composition.

[0016] Preferably, the amount of water is 5-15 times the total mass of the raw materials, the extraction is decoction, the number of decoctions is 1-3 times, the decoction time each time is 0.5-2 hours, and the decoction temperature is 95-105°C.

[0017] Preferably, the decoction is followed by filtration, the filtrates are combined, the filtrates are decompressed, and the filtrates are concentrated to obtain the product;

[0018] Preferably, the concentration is to concentrate until the filtrate concentration is 1 g crude drug equivalent / mL; and sterilization is performed after the concentration.

[0019] The third object of the present invention is to provide an antibacterial preparation, the raw materials of which include the above-mentioned composition.

[0020] Another object of the present invention is to provide the use of the above composition or the composition prepared by the above preparation method or the above antibacterial preparation in inhibiting oral pathogens;

[0021] Preferably, the oral pathogens include Streptococcus mutans and / or Porphyromonas gingivalis.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] (1) The composition provided by the present invention uses appropriate raw materials and proportions, and achieves a good effect of inhibiting oral pathogens compared to using a single raw material or other medicinal materials.

[0024] (2) The present study found that the combination of peony bark and cloves, and peony bark and licorice showed synergistic effects on Streptococcus mutans and Porphyromonas gingivalis, respectively; the combination of cloves, peony bark and licorice had a synergistic inhibitory effect on oral pathogens Streptococcus mutans and Porphyromonas gingivalis, with the MIC against Streptococcus mutans being 3.91 mg / mL and the MIC against Porphyromonas gingivalis being 15.63 mg / mL. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 The graph is a graph showing the antibacterial growth of Streptococcus mutans in Example 4.

[0026] Figure 2 This is the antibacterial growth curve of Example 4 against Porphyromonas gingivalis. DETAILED DESCRIPTION

[0027] The technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Although the representative embodiments of the present invention have been described in detail, it will be understood by those skilled in the art that various modifications and changes can be made to the present invention without departing from the scope of the present invention. Therefore, the scope of the present invention should not be limited to the embodiments, but should be limited by the appended claims and their equivalents.

[0028] Experimental Materials

[0029] Licorice (lot number: C23060103) and mulberry leaf (lot number: C201224263) were purchased from Anguo Runde Pharmaceutical Co., Ltd.; honeysuckle (lot number: 2212009), atractylodes (lot number: 2309003), angelica dahurica (lot number: 2309001), and mint (lot number: 2311004) were purchased from Jiangxi Zhangshu Tianqitang Chinese Medicine Pieces Co., Ltd.; clove (lot number: 231101) and peony bark (lot number: 231101) were purchased from Jiangxi Heshuo Pharmaceutical Co., Ltd.; HB8297-5 brain heart infusion broth was purchased from Jiangxi Heshuo Pharmaceutical Co., Ltd. Culture medium (Qingdao Hi-Tech Industrial Park Haibo Biotechnology Co., Ltd., 20230525); CM107 nutrient agar (Beijing Luqiao Technology Co., Ltd., 2302242); HB0124 Columbia blood agar medium (Qingdao Hi-Tech Industrial Park Haibo Biotechnology Co., Ltd., 20230831); Streptococcus mutans (SM, ATCC25175) was purchased from Guangdong Provincial Center for Microbiological Culture Collection; Porphyromonas gingivalis (PG, ATCC33277) was purchased from Shanghai Center for Microbiological Culture Collection.

[0030] Instruments and Equipment

[0031] HFsafe-1500LC biological safety cabinet, Shanghai Likang Scientific Instrument Co., Ltd.; ZWY-200D constant temperature oscillating incubator, Shanghai Zhichu Instrument Co., Ltd.; LDZF-50L-111 vertical pressure steam autoclave, Shanghai Shen'an Medical Instrument Factory; Eppendorf pipette, Nanjing Beden Medical Co., Ltd.; XW-80A micro vortex mixer, Shanghai Huxi Instrument Factory Co., Ltd.; ENSIGHT multifunctional microplate reader, PerkinElmer, Singapore; BP-2200-1 growth curve analyzer, Bioscreen C, Finland; NEST 96-well plate, Beijing Biotech Co., Ltd.; 6 mm Oxford cup, Beijing Jitaiyuancheng Technology Co., Ltd.; BCE6202i-iCCN one-hundredth balance, Sartorius, Germany; SXKW digital display temperature-controlled electric heating mantle, Beijing Yongguangming Medical; RE-6000A rotary evaporator, IKA, Germany.

[0032] Example 1

[0033] Raw materials of composition 1: peony bark and liquorice, with a mass ratio of 2:1.

[0034] Preparation: Weigh peony bark and liquorice root separately, add 10 times the amount of water, and boil twice for 1 hour each time. Filter and collect the filtrates. Concentrate the filtrates under reduced pressure to a concentration of 1.0 g crude drug equivalent / mL. Sterilize by autoclaving, cool, and refrigerate at 4°C until ready for use.

[0035] Example 2

[0036] Raw materials of composition 2: peony bark and cloves, with a mass ratio of 1:2.

[0037] Preparation: Weigh peony bark and cloves separately, add 10 times the amount of water, and boil twice for 1 hour each time. Filter and collect the filtrates. Concentrate the filtrates under reduced pressure to a concentration of 1.0 g crude drug equivalent / mL. Sterilize by autoclaving, cool, and refrigerate at 4°C until ready for use.

[0038] Example 3

[0039] Raw materials of composition 3: peony bark, cloves and liquorice, with a mass ratio of 4:1:4.

[0040] Preparation: Weigh peony bark, cloves, and licorice separately, add 10 times the amount of water, and boil twice for 1 hour each time. Filter and collect the filtrates. Concentrate the filtrates under reduced pressure to 1.0 g of crude drug equivalent / mL. Sterilize by autoclaving, cool, and refrigerate at 4°C until ready for use.

[0041] Example 4

[0042] Raw materials of composition 4: peony bark, cloves and liquorice, with a mass ratio of 2:8:1.

[0043] Preparation: Weigh peony bark, cloves, and licorice root separately, add 10 times the amount of water, and boil twice for 1 hour each time. Filter and collect the filtrates. Concentrate the filtrates under reduced pressure to a concentration of 1.0 g crude drug equivalent / mL. Sterilize by autoclaving, cool, and refrigerate at 4°C until ready for use.

[0044] Example 5

[0045] Raw materials of composition 5: peony bark, cloves and liquorice, with a mass ratio of 4:1:4.

[0046] Preparation: Weigh peony bark, clove, and licorice separately, add 10 times the amount of water, and boil twice for 1 hour each time. Filter and collect the filtrates. Concentrate the filtrates under reduced pressure to a concentration of 1.0 g crude drug equivalent / mL to obtain the peony bark, clove, and licorice extracts. Combine the three extracts to obtain the extract. Autoclave, cool, and refrigerate at 4°C until ready for use.

[0047] Comparative Example 1

[0048] The raw material is single peony bark, and the preparation method is the same as that in Example 3.

[0049] Comparative Example 2

[0050] The raw material is single liquorice, and the preparation method is the same as that in Example 3.

[0051] Comparative Example 3

[0052] The raw material is single clove, and the preparation method is the same as that in Example 3.

[0053] Experiment 1: Antibacterial activity test of the Chinese medicine composition of the present invention

[0054] 1. Preparation of Bacterial Suspension

[0055] Glycerol tubes of Streptococcus mutans and Porphyromonas gingivalis (-80°C) were taken and activated twice in brain heart infusion broth. After anaerobically incubated at 37°C for 16 h, they were diluted to approximately 1×10 6 ~5×10 6 The bacterial suspension with a CFU / mL was used as the experimental bacterial solution.

[0056] 2. Inhibition Zone Determination

[0057] The Oxford cup method was used to test the antibacterial properties of single Chinese herbal medicines. 200 μL each of the Streptococcus mutans and Porphyromonas gingivalis suspensions prepared above were plated onto nutrient agar and Columbia blood agar plates, respectively, and evenly spread using a disposable spreading stick. After drying, an Oxford cup (6 mm diameter) was placed on the plate using tweezers. 200 μL each of the single Chinese herbal extracts prepared in Comparative Examples 1-3 (Paeonia suffruticosa, Licorice, and Clove) was pipetted into the cup. The cups were labeled and incubated anaerobically at 37°C for 24 hours. The results were observed and the inhibition zones were measured. The test was repeated three times. The criteria for determining the inhibition zone were: an average inhibition zone diameter >20 mm, indicating extreme sensitivity; an average inhibition zone diameter of 15-20 mm, indicating high sensitivity; an average inhibition zone diameter of 10-15 mm, indicating moderate sensitivity; an average inhibition zone diameter <10 mm, indicating low sensitivity; and no inhibition zone, indicating insensitivity. The results are shown in Table 1.

[0058] Table 1

[0059]

[0060] 3. Minimum Inhibitory Concentration Determination

[0061] The experimental group received a TCM aqueous extract at a concentration of 3.91-500 mg / mL, while the negative control group received sterile water instead of the drug. A blank control group was also established without the addition of the drug. Both experimental and control groups were incubated in an anaerobic incubator at 37°C for 18 hours, followed by determination of OD600 values using a microplate reader. The minimum TCM concentration at which the OD600 value of the experimental group was not significantly different from that of the blank control group and the negative control group was defined as the MIC. The experiment was repeated three times in parallel. The results showed that Paeonia suffruticosa, Clove, and Licorice had significant antibacterial effects against two oral pathogens (see Table 2).

[0062] Table 2

[0063]

[0064] 4. Determination of the antibacterial activity of three Chinese herbal medicine combinations using the chessboard method

[0065] Based on the Oxford cup experiment and the MIC experiment, the checkerboard method was used to detect the inhibitory effects of the pairwise combinations of the above-mentioned Cortex Moutan, Flos Caryophylli, and Radix Glycyrrhizae on two pathogenic bacteria. The specific method was to add the diluted aqueous extracts of traditional Chinese medicine horizontally and vertically respectively, so that the total concentration in each well was 2, 1, 1 / 2, 1 / 4, 1 / 8 MIC. Two-dimensional checkerboard method: It is known that FIC = MIC A Combined use / MIC A Single use + MIC B Combined use / MIC B Single use of the drug. When FIC < 0.5, it is a synergistic effect; when 0.5 < FIC < 1.0, it is an additive effect; when 1.0 < FIC < 2.0, it is an irrelevant effect; when FIC > 2.0, it is an antagonistic effect. The antibacterial results of the combination of Cortex Moutan and Flos Caryophylli, and the combination of Cortex Moutan and Radix Glycyrrhizae against Streptococcus mutans and Porphyromonas gingivalis are shown in Tables 3, 4, 7, and 8. In addition, the antibacterial results of the combination of Radix Glycyrrhizae and Flos Caryophylli against Streptococcus mutans and Porphyromonas gingivalis are shown in Tables 5 and 6.

[0066] The two-dimensional checkerboard method was upgraded to a three-dimensional checkerboard method to detect the inhibitory effects of the combination of three herbs, Cortex Moutan, Flos Caryophylli, and Radix Glycyrrhizae, on two pathogenic bacteria. The specific method was to adjust the concentration according to the MIC value of each extract. The first two extracts were added horizontally and vertically to a 96-well plate, and the concentrations were adjusted to 1 / 2 MIC, 1 / 4 MIC, 1 / 8 MIC, and 1 / 16 MIC. Finally, the third drug was added and the concentration was adjusted to 1 / 2 MIC, 1 / 4 MIC, 1 / 8 MIC, and 1 / 16 MIC. So that each drug accounted for 1 / 4, 1 / 8, 1 / 16, and 1 / 32 MIC of the concentration in each well. According to the checkerboard method experiment, experimental groups, blank controls, and negative controls were set up. After anaerobic culture at 37 °C for 18 h, OD600 was measured and the combined MIC value was calculated. The results showed that the MIC against Streptococcus mutans was 3.91 mg / mL, and the MIC against Porphyromonas gingivalis was 15.63 mg / mL. The specific antibacterial results are shown in Tables 9 - 16.

[0067] It is known that for the combined antibacterial index FIC, the three-drug combination gives FIC = MIC A Combined use / MIC A Single use + MIC B Combined use / MIC B Single use of the drug + MIC C Combined use / MIC C Single use of the drug. When FIC < 0.5, it is a synergistic effect; when 0.5 < FIC < 1.0, it is an additive effect; when 1.0 < FIC < 2.0, it is an irrelevant effect; when FIC > 2.0, it is an antagonistic effect.

[0068] Table 3 Antibacterial situation of drug combinations against Streptococcus mutans

[0069]

[0070] Note: “+” indicates bacterial growth and “-” indicates no bacterial growth.

[0071] Table 4 Antibacterial effect of drug combination on Porphyromonas gingivalis

[0072]

[0073] Note: “+” indicates bacterial growth and “-” indicates no bacterial growth.

[0074] Table 5 Antibacterial effect of drug combination on Streptococcus mutans

[0075]

[0076] Note: “+” indicates bacterial growth and “-” indicates no bacterial growth.

[0077] Table 6 Antibacterial effect of drug combination on Porphyromonas gingivalis

[0078]

[0079] Note: “+” indicates bacterial growth and “-” indicates no bacterial growth.

[0080] Table 7 Antibacterial effect of drug combination on Streptococcus mutans

[0081]

[0082] Note: “+” indicates bacterial growth and “-” indicates no bacterial growth.

[0083] Table 8 Antibacterial effect of drug combination on Porphyromonas gingivalis

[0084]

[0085] Note: “+” indicates bacterial growth and “-” indicates no bacterial growth.

[0086] Table 9 Inhibition of three drugs combined against Streptococcus mutans

[0087]

[0088] Note: “+” indicates bacterial growth and “-” indicates no bacterial growth.

[0089] Table 10 Inhibition of three drugs combined against Streptococcus mutans

[0090]

[0091] Note: “+” indicates bacterial growth and “-” indicates no bacterial growth.

[0092] Table 11 Inhibitory effect of three drugs combined on Streptococcus mutans

[0093]

[0094] Note: “+” indicates bacterial growth and “-” indicates no bacterial growth.

[0095] Table 12 Inhibition of three drugs combined against Streptococcus mutans

[0096]

[0097] Note: “+” indicates bacterial growth and “-” indicates no bacterial growth.

[0098] Table 13 Inhibitory effect of three drugs combined on Porphyromonas gingivalis

[0099]

[0100] Note: “+” indicates bacterial growth and “-” indicates no bacterial growth.

[0101] Table 14 Inhibitory effect of three drugs combined on Porphyromonas gingivalis

[0102]

[0103] Note: “+” indicates bacterial growth and “-” indicates no bacterial growth.

[0104] Table 15 Inhibitory effect of three drugs combined on Porphyromonas gingivalis

[0105]

[0106] Note: “+” indicates bacterial growth and “-” indicates no bacterial growth.

[0107] Table 16 Inhibitory effect of three drugs combined on Porphyromonas gingivalis

[0108]

[0109] Note: “+” indicates bacterial growth and “-” indicates no bacterial growth.

[0110] Tables 3-8 show that the most effective combination with peony bark is licorice and clove; the most effective combination with clove is peony bark; and the most effective combination with licorice is peony bark and clove. The combination of peony bark and clove has the best antibacterial effect, with an FIC of 0.38, exhibiting a synergistic effect against Streptococcus mutans. The combination of peony bark and licorice has the best antibacterial effect, with an FIC of 0.38, exhibiting a synergistic effect against Porphyromonas gingivalis. However, the combination of any two Chinese herbal extracts did not exhibit synergistic effects against both bacteria.

[0111] Considering the antibacterial effects of a combination of aqueous extracts of three traditional Chinese medicines, experimental data were processed: the absorbance of the experimental group minus the absorbance of the blank group equals the OD600 of each drug concentration against Streptococcus mutans and Porphyromonas gingivalis. The results were then compared with the negative control group. No significant differences were considered to indicate a lack of bacterial growth. The results showed that the combination of peony bark, clove, and licorice had a synergistic antibacterial effect against both bacteria. The optimal mass ratio of the three herbs was peony bark: clove: licorice = 4:1:4 or 2:8:1, resulting in an FICI of 0.34, as shown in Tables 9-16.

[0112] 5. Verification of the antibacterial growth curve of the aqueous extract of the Chinese herbal medicine combination formula against two bacteria

[0113] According to the ratio and preparation method of Examples 1-5, the aqueous extract (1.0 mg / mL) of the antibacterial compatibility formula of Examples 1-5, which has a synergistic effect on two pathogenic bacteria, was prepared. Take a sterile honeycomb microplate and use a micropipette to add 300 μL of the aqueous extract of the combined formula diluted with brain heart infusion broth to a concentration of 500 mg / mL in well 11. Add 150 μL of brain heart infusion broth to wells 12 to 18 and perform micro-two-fold dilution with a pipette. Discard the last well and add 150 μL of bacterial solution to each well. The same method is used for wells 21-28 and 31-38. Wells 01-03 are negative controls and 150 μL of mutans Streptococcus bacterial solution and 150 μL of sterile water are added. Take another sterile honeycomb microplate as above and change the bacterial solution to Porphyromonas gingivalis. The rest of the samples remain unchanged. Because Porphyromonas gingivalis is anaerobic, 50 μL of liquid paraffin is added to each well after all the drug solution and bacterial culture medium are added and anaerobically sealed. The two well plates were placed in a fully automatic growth curve instrument and cultured for 24 h. The OD600 was measured every hour. The experiment was repeated three times.

[0114] The results showed that the Chinese medicine composition prepared by the mixed extraction of the above Chinese medicines also showed significant synergistic antibacterial effect. The antibacterial growth curve results of Example 4 are shown in Figure 1-Figure 2 .

[0115] Clove, peony bark, and licorice have inhibitory effects on oral pathogens. The MICs of clove, peony bark, and licorice against Streptococcus mutans were 3.91 mg / mL, and against Porphyromonas gingivalis were 15.63 mg / mL. Combinations of peony bark and clove, and peony bark and licorice, showed additive effects against S. mutans and P. gingivalis, respectively. A combination of these three Chinese herbal extracts achieved synergistic inhibition against both bacteria, effectively suppressing oral pathogens. At present, there has been no research on the antibacterial Chinese herbal compound for inhibiting Streptococcus mutans and Porphyromonas gingivalis and its effects. The present invention explores the antibacterial effects of Chinese herbal water extracts such as peony bark, cloves and licorice, as well as the compound, on Streptococcus mutans and Porphyromonas gingivalis. It is expected that peony bark, cloves and licorice can be put into actual production applications, providing a theoretical basis for the clinical application of Chinese herbal medicines such as peony bark, cloves and licorice, and conducting basic research for the research and development of Chinese herbal oral health products with antibacterial effects.

[0116] 6. Antibacterial results of different ratios of peony bark, clove and liquorice compositions

[0117] The FICI results of peony bark, clove and licorice showing synergistic inhibitory effects on Streptococcus mutans and Porphyromonas gingivalis are shown in Table 17.

[0118] Table 17

[0119]

[0120] Experiment 2: Antibacterial Test of Other Traditional Chinese Medicines

[0121] In addition, the present invention also provides other comparative examples to conduct antibacterial tests on other traditional Chinese medicines. The test method is the same as the above-mentioned "Experiment 1". The results of the antibacterial zone test are shown in Table 18 below.

[0122] Table 18

[0123]

[0124] The MIC value determination results of Atractylodes macrocephala are shown in Table 19.

[0125] Table 19

[0126]

[0127] The above detailed description is a specific description of one feasible embodiment of the present invention. This embodiment is not intended to limit the patent scope of the present invention. Any equivalent implementation or modification that does not depart from the present invention should be included in the scope of the technical solution of the present invention.

Claims

1. A composition for inhibiting oral pathogens, the composition comprising peony bark, cloves, and liquorice in a mass ratio of 0.25-8:0.25-8:1; The method for preparing the composition for inhibiting oral pathogens comprises extracting the raw materials of the composition with water separately and mixing the extracts to obtain the composition; or mixing the raw materials of the composition and extracting with water to obtain the composition; The amount of water used is 5-15 times the total mass of the raw materials. The extraction is performed by decoction. The number of decoctions is 1-3 times. The decoction time for each decoction is 0.5-2 hours. The decoction temperature is 95-105°C.

2. The composition according to claim 1, characterized in that After the decoction, the decoction is filtered, the filtrates are combined, the filtrates are decompressed, and the decompression is performed to obtain the product; the concentration is performed until the filtrate concentration is 1 g of crude drug equivalent / mL; and the product is sterilized after the concentration.

3. Use of the composition according to any one of claims 1 to 2 in the preparation of a product for inhibiting oral pathogens.

4. The use according to claim 3, characterized in that The oral pathogens include Streptococcus mutans and / or Porphyromonas gingivalis.

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