A crRNA target site and crisp-cas13a system for detecting hendra virus
By combining the CRISPR-Cas13a system with lateral flow chromatography test strips, a specific crRNA targeting Hendra virus nucleic acid was designed, solving the problem that existing detection methods cannot maintain high sensitivity and high specificity in a rapid and simple manner, and realizing high-sensitivity detection of Hendra virus nucleic acid.
CN119431522BActive Publication Date: 2026-06-05ACADEMY OF MILITARY MEDICAL SCIENCES
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- ACADEMY OF MILITARY MEDICAL SCIENCES
- Filing Date
- 2024-12-18
- Publication Date
- 2026-06-05
AI Technical Summary
Technical Problem
Existing Hendra virus detection methods cannot maintain high sensitivity and specificity while being rapid and convenient, making it difficult to meet the needs of early diagnosis.
Method used
A detection method combining the CRISPR-Cas13a system with lateral flow chromatography strips was developed. Specific crRNA targeting Hendra virus nucleic acid was designed, and high-sensitivity detection was achieved through activation by the CRISPR-Cas13a system.
Benefits of technology
It achieves rapid, simple, highly sensitive, and highly specific detection of Hendra virus nucleic acid, with a detection sensitivity of one copy/μL.
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Smart Images
Figure CN119431522B_ABST
Abstract
The application discloses a crRNA target point and a CRISPR-Cas13a system for detecting Hendra virus, wherein the CRISPR-Cas13a system comprises a Cas13a protein and a crRNA, or a complex formed by both; the crRNA comprises an anchor sequence for being combined with the Cas13a protein and a guide sequence for targeting a Hendra virus target sequence; and the Hendra virus target sequence is located at the 632th-659th position of a Hendra virus genome. Experiments prove that the crRNA can realize high-sensitivity and high-specificity detection on the nucleic acid of the Hendra virus by activating the Cas13a, and the sensitivity reaches one copy (1 copy / muL); and the application has important application value.
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