Liquid phase low pressure gradient pump multi-cycle distribution method and computer equipment

Abstract

The present application provides a multi-cycle allocation method of a liquid-phase low-pressure gradient pump and a computer device, which belongs to the technical field of liquid chromatographs. The method includes: determining whether the liquid chromatograph currently meets the cycle conditions according to the operating state of the infusion pump in the liquid chromatograph; if so, determining the actual number of cycles according to the target solvent ratio in the target sample solution, the flow rate of the infusion pump, the preset reference cycle factor, the number of cycles corresponding to the preset reference cycle factor, and the preset upper limit of the number of cycles; determining whether the liquid chromatograph is currently in the allocation cycle according to the operating state of the liquid chromatograph and the actual number of cycles; if so, determining the allocation cycle ratio corresponding to the allocation cycle according to the target solvent ratio in the target sample solution and the actual number of cycles, and extracting liquid within the allocation cycle according to the allocation cycle ratio. The present application can achieve the effect of optimizing the accuracy and repeatability of the solvent ratio of small proportions or large flow rates, thereby improving the accuracy and reliability of the liquid chromatograph.

Description

Technical Field

[0001] The present application relates to the technical field of liquid chromatographs, and in particular to a multi-cycle distribution method of a liquid phase low-pressure gradient pump and a computer device. Background Art

[0002] Liquid chromatograph is an instrument based on liquid chromatography technology. It is highly favored in biomedicine, environmental monitoring, food safety, petrochemical and other fields due to its strong separation ability, fast analysis speed and high detection sensitivity.

[0003] In the related art, a plurality of quantitative solvents are often mixed through a liquid chromatograph to obtain a sample solution that meets the needs of the user. However, when the infusion pump is used to extract liquid based on the related art, at the same flow rate, the smaller the proportion of the solvent in the sample solution, the shorter the extraction time required, and the switching error of the gradient valve will seriously affect the extraction accuracy of the solvent, thereby affecting the accuracy and reliability of the sample solution. In addition, if the extraction speed of the infusion pump is faster, the extraction time required for each solvent will be shorter, and the switching error of the gradient valve will also seriously affect the extraction accuracy of each solvent, thereby affecting the accuracy and reliability of the sample solution. Therefore, the solution of the related art has the problem that the extraction accuracy of the liquid chromatograph is low, and the accuracy and reliability of the sample solution obtained by mixing the liquid chromatograph are also low. Summary of the invention

[0004] The purpose of the present application is to provide a liquid phase low-pressure gradient pump multi-cycle distribution method and computer equipment, which can optimize the accuracy and repeatability of solvent ratios with small proportions or large flow rates, thereby improving the accuracy and reliability of liquid chromatographs.

[0005] The embodiment of the present application is implemented as follows:

[0006] In a first aspect of an embodiment of the present application, a liquid phase low-pressure gradient pump multi-cycle distribution method is provided, the method comprising:

[0007] Obtaining the operating status of the liquid chromatograph, and determining whether the liquid chromatograph meets the cycle conditions at the current moment according to the operating status;

[0008] If yes, the actual number of cycles is determined according to the target solvent ratio in the target sample solution, the flow rate of the infusion pump, the preset reference cycle factor, the number of cycles corresponding to the preset reference cycle factor, and the preset upper limit of the number of cycles;

[0009] According to the running state of the liquid chromatograph and the actual number of divided weeks, determine whether the liquid chromatograph is currently in the allocated week;

[0010] If so, the allocation week ratio corresponding to the allocation week is determined according to the target solvent ratio in the target sample solution and the actual number of divided weeks, and the solvents required for the target sample solution are extracted within the allocation week according to the allocation week ratio.

[0011] As a possible implementation method, the actual number of cycles is determined according to the target solvent ratio in the target sample solution, the flow rate of the infusion pump, the preset reference cycle factor, the number of cycles corresponding to the preset reference cycle factor, and the preset upper limit of the number of cycles, including:

[0012] Determine the current cycle factor according to the target solvent ratio in the target sample solution and the flow rate of the infusion pump;

[0013] Determine the theoretical number of weeks according to the current week division factor, the preset reference week division factor and the number of weeks corresponding to the preset reference week division factor;

[0014] The actual number of weeks is determined based on the rounded-down result of the theoretical number of weeks and the preset upper limit of the number of weeks.

[0015] As a possible implementation method, the current cycle factor is determined according to the target solvent ratio in the target sample solution and the flow rate of the infusion pump, including:

[0016] Calculating a first product between a target solvent ratio in a target sample solution and a preset threshold value;

[0017] calculating a first ratio between the flow rate of the infusion pump and the first product;

[0018] The current cycle factor is determined according to the first ratio.

[0019] As a possible implementation, the theoretical number of divided weeks is determined according to the current divided week factor, the preset reference divided week factor, and the number of divided weeks corresponding to the preset reference divided week factor, including:

[0020] Calculating a second ratio between the current clocking factor and a preset reference clocking factor;

[0021] Calculating a second product between the second ratio and the number of division cycles corresponding to a preset reference division cycle factor;

[0022] Based on the second product, determine the theoretical number of sub-cycles.

[0023] As a possible implementation method, the actual number of divided weeks is determined according to the result of rounding down the theoretical number of divided weeks and the preset upper limit of the number of divided weeks, including:

[0024] If the result of rounding down the theoretical number of divided weeks is greater than the preset upper limit of the number of divided weeks, the actual number of divided weeks is determined to be the preset upper limit of the number of divided weeks;

[0025] If the rounded-down result of the theoretical divided week number is less than or equal to the preset divided week number upper limit value, the actual divided week number is determined to be the rounded-down result of the theoretical divided week number.

[0026] As a possible implementation method, according to the target solvent ratio in the target sample solution and the actual number of divided weeks, the allocation week ratio corresponding to the allocation week is determined, including:

[0027] Calculate the third product between the target solvent ratio in the target sample solution and the actual number of divided cycles;

[0028] Based on the third product, the allocation week ratio corresponding to the allocation week is determined.

[0029] As a possible implementation method, the above-mentioned liquid phase low-pressure gradient multi-cycle distribution method also includes:

[0030] If the liquid chromatograph is not currently in a dispensing cycle, the extraction of the target solvent is stopped.

[0031] As a possible implementation method, the above-mentioned liquid phase low-pressure gradient multi-cycle distribution method also includes:

[0032] Determine the channel flow rate corresponding to the target solvent in the target sample solution according to the target solvent ratio in the target sample solution and the flow rate of the infusion pump;

[0033] The duration of extracting the target solvent from the target sample solution is determined according to the target solvent ratio in the target sample solution and the channel flow rate.

[0034] As a possible implementation method, according to the target solvent ratio in the target sample solution and the flow rate of the infusion pump, the channel flow rate corresponding to the target solvent in the target sample solution is determined, including:

[0035] calculating a fourth product between the flow rate of the infusion pump and the proportion of the target solvent in the target sample solution;

[0036] The channel flow rate corresponding to the target solvent in the target sample solution is determined according to the fourth product.

[0037] According to a second aspect of an embodiment of the present application, a computer device is provided, which includes: a memory and a processor, wherein the memory stores a computer program that can be run on the processor, and when the processor executes the computer program, the steps of the liquid-phase low-pressure gradient multi-cycle distribution method described in the first aspect are implemented.

[0038] According to a third aspect of an embodiment of the present application, a computer-readable storage medium is provided, on which a computer program is stored. When the computer program is executed by a processor, the steps of the liquid-phase low-pressure gradient multi-cycle distribution method described in the first aspect are implemented.

[0039] The beneficial effects of the embodiments of the present application include:

[0040] The embodiment of the present application provides a liquid phase low-pressure gradient multi-cycle allocation method, which obtains the operating state of a liquid chromatograph, determines the operating state of an infusion pump in the liquid chromatograph according to the operating state of the liquid chromatograph, and judges whether the liquid chromatograph meets the cycle conditions at the current moment according to the operating state of the infusion pump in the liquid chromatograph; if so, the actual cycle number is calculated according to the target solvent ratio required by the target sample solution, the flow rate in the liquid chromatograph, the preset reference cycle factor, the number of cycles corresponding to the preset reference cycle factor, and the preset upper limit of the number of cycles; the entire liquid extraction stroke of the liquid chromatograph is processed according to the actual cycle number to obtain the liquid extraction cycle result of the liquid chromatograph, and judges whether the liquid chromatograph reaches the allocation cycle according to the liquid extraction cycle result of the liquid chromatograph and the operating state of the liquid chromatograph; if so, the allocation cycle ratio corresponding to the allocation cycle is calculated according to the target solvent ratio required by the target sample solution and the actual cycle number, so as to control the infusion pump in the liquid chromatograph to extract each solvent required by the target sample solution within the allocation cycle according to the allocation cycle ratio. Among them, the entire extraction stroke of the liquid chromatograph is converted into the time dimension by introducing a division factor, and the actual division number is calculated according to the solvent ratio of the target solvent with a smaller proportion required by the target sample solution and the preset division factor and other reference parameters. The entire extraction stroke of the liquid chromatograph is divided into periods based on the actual division number, and each solvent required by the target sample solution is drawn according to the distribution week ratio within the distribution week, so that the target solvent with a smaller proportion of solvent is fully extracted within the distribution week, so as to extend the single extraction time of the target solvent, thereby reducing the switching error of the channel valve where the target solvent with a smaller proportion of solvent is located, and improving the extraction accuracy of the target solvent. In this way, the accuracy and repeatability of the solvent ratio of small proportion or large flow rate can be optimized, thereby improving the accuracy and reliability of the liquid chromatograph. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present application and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.

[0042] Figure 1 It is a working principle diagram of an existing liquid chromatograph;

[0043] Figure 2 It is a schematic diagram of a complete liquid extraction stroke of an existing liquid chromatograph;

[0044] Figure 3 A flow chart of a first liquid phase low pressure gradient pump multi-cycle distribution method provided in an embodiment of the present application;

[0045] Figure 4 A flow chart of a second liquid-phase low-pressure gradient pump multi-cycle distribution method provided in an embodiment of the present application;

[0046] Figure 5 A flow chart of a third liquid phase low pressure gradient pump multi-cycle distribution method provided in an embodiment of the present application;

[0047] Figure 6 A flowchart of a fourth liquid-phase low-pressure gradient pump multi-cycle distribution method provided in an embodiment of the present application;

[0048] Figure 7 A working flow chart of a multi-cycle distribution method of a liquid phase low-pressure gradient pump provided in an embodiment of the present application;

[0049] Figure 8 This is an implementation effect diagram of a liquid phase low-pressure gradient pump multi-cycle distribution method provided in an embodiment of the present application;

[0050] Fig. 9 A schematic diagram of the structure of a computer device provided in an embodiment of the present application. DETAILED DESCRIPTION

[0051] In order to make the purpose, technical solution and advantages of the embodiments of the present application clearer, the technical solution in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. The components of the embodiments of the present application described and shown in the drawings here can be arranged and designed in various different configurations.

[0052] Therefore, the following detailed description of the embodiments of the present application provided in the accompanying drawings is not intended to limit the scope of the present application for which protection is sought, but merely represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in the field without creative work are within the scope of protection of the present application.

[0053] At present, multiple quantitative solvents are often mixed through a liquid chromatograph to obtain a sample solution that meets user needs. However, when the infusion pump is pumping liquid based on this scheme, at the same flow rate, the smaller the proportion of the solvent in the sample solution, the shorter the pumping time required. The switching error of the quaternary gradient valve will seriously affect the pumping accuracy of the solvent, thereby affecting the accuracy and reliability of the sample solution. In addition, when the proportions of each solvent required for the sample solution are the same, the faster the pumping speed of the infusion pump, the shorter the pumping time required for each solvent. The switching error of the quaternary gradient valve will seriously affect the pumping progress of each solvent, thereby affecting the accuracy and reliability of the sample solution.

[0054] To this end, the embodiment of the present application provides a multi-cycle allocation method for a liquid phase low-pressure gradient pump, by obtaining the operating state of the liquid chromatograph at the current moment, and determining whether the liquid chromatograph meets the conditions for the weekly calculation at that moment according to the operating state of the liquid chromatograph at the current moment; if so, the actual number of weekly divisions is calculated according to the target solvent ratio in the target sample solution, the flow rate of the infusion pump, the preset reference weekly division factor, the number of weekly divisions corresponding to the preset reference weekly division factor, and the preset upper limit of the number of weekly divisions; according to the operating state of the liquid chromatograph and the calculated actual number of weekly divisions, determine whether the liquid chromatograph is currently in the allocation cycle; if so, according to the target solvent ratio in the target sample solution and the actual number of weekly divisions, calculate the allocation cycle ratio corresponding to the allocation cycle, and control the infusion pump in the liquid chromatograph to extract the solvents required for the target sample solution according to the allocation cycle ratio. Among them, the present application unifies the extraction process of each solvent contained in the target sample solution in the time dimension by introducing the weekly division factor. In this way, the accuracy and repeatability of the solvent ratio at a small ratio or a large flow rate can be optimized, thereby improving the accuracy and reliability of the liquid chromatograph.

[0055] Figure 1 is a working principle diagram of an existing liquid chromatograph, see Figure 1 The existing liquid chromatograph includes: a vacuum degassing chamber, a gradient valve, an inlet check valve, an outlet check valve, a mixing damper, and a vent valve. Among them, the gradient valve can be a ternary gradient valve, a quaternary gradient valve, etc. This application takes a quaternary gradient valve as an example, but it does not mean that the gradient valve can only be a quaternary gradient valve, and this application does not make a specific limitation on this.

[0056] Optionally, the vacuum degassing chamber can remove the gas in the mobile phase of the liquid chromatograph to avoid the influence of bubbles on the analysis results. The vacuum degassing chamber eliminates oxygen, nitrogen and other gases in the mobile phase of the liquid chromatograph by heating and vacuuming to avoid gas dissolution and degradation of the stationary phase of the chromatographic column, thereby affecting the separation performance of the liquid chromatograph; the quaternary gradient valve is mainly used to control the proportion of the mobile phase to achieve gradient elution, thereby improving the separation effect and analysis efficiency of the liquid chromatograph. The quaternary gradient valve can run the mobile phase of four channels at the same time, and achieve different proportions of mobile phase mixing through the switch control of the solenoid valve. The quaternary gradient valve can automatically adjust the proportion of the mobile phase under a preset program to achieve gradient elution; the inlet check valve is mainly used to prevent the backflow of the medium to protect the infusion pump and the drive motor from reversal damage. The inlet check valve, based on its mechanical structure, allows the fluid to flow only in a single phase to prevent the medium from flowing back during the liquid chromatography separation process. It can effectively avoid cross-contamination between samples and washing liquid, thereby improving the separation accuracy of the liquid chromatograph. The inlet check valve can also prevent leakage of container media, thereby improving the sealing and stability of the liquid chromatograph. The outlet check valve is mainly used to prevent medium backflow, protect the infusion pump and the drive motor from reverse damage, and prevent leakage of the medium in the container. The outlet check valve uses the flow power of the medium itself to realize the automatic opening and closing of the valve disc, thereby ensuring that the fluid can only flow in one direction and avoiding damage to the liquid chromatograph caused by reverse flow of the fluid. The mixing damper is mainly used to reduce the pressure pulse generated by the infusion pump during movement to stabilize the liquid flow rate in the liquid chromatograph to ensure the accuracy of the sample solution mixed by the liquid chromatograph. The vent valve serves as the maintenance interface of the liquid chromatograph. The vent valve can be an automatic vent valve or a manual vent valve. The vent valve is mainly used to remove bubbles in the solvent in the infusion pump to maintain the stability of the system.

[0057] Optionally, the quaternary gradient valve is composed of four solenoid valves assembled on a cubic five-way valve, each solenoid valve controlling the mobile phase of a channel. When the quaternary gradient valve is working, the mainboard program controls the power supply of each solenoid valve, and controls the proportion of the mobile phase by adjusting the switching time of the solenoid valve.

[0058] Optionally, the outlet check valve can control the flow direction of the liquid between the inlet and outlet during the liquid chromatograph's liquid chromatographic analysis to prevent cross-infection between the sample solution and the washing solution; the outlet check valve can also reduce the noise and peak shape deformation generated when the liquid chromatograph is disturbed by the external environment, thereby improving the separation efficiency of the liquid chromatograph. In addition, to ensure the good performance of the outlet check valve, the outlet check valve needs to be cleaned and maintained regularly.

[0059] Optionally, the hybrid damper prevents pressure fluctuations through the pressure changes of the internal pressure sensor and all subsequent pipelines. When the infusion pump in the liquid chromatograph moves, corresponding pressure pulses will be generated. These pressure pulses will cause the liquid flow rate in the liquid chromatograph to be unstable, thereby affecting the separation effect and detection accuracy of the liquid chromatograph. The hybrid damper is used to eliminate the pressure pulses generated by the movement of the infusion pump, thereby ensuring the stability of the liquid flow rate in the liquid chromatograph. In addition, the hybrid damper can adjust the liquid flow rate by controlling the pressure at the inlet of the chromatographic column to avoid damage to the chromatographic column due to excessive flow rate.

[0060] ‌‌‌Optionally, in a liquid chromatograph, the sample solution enters the chromatographic column through the injection port for analysis and analysis. After the sample undergoes multiple distributions on the stationary phase of the chromatographic column, different components are separated. However, the distribution process causes the solvent to accumulate on the stationary phase, thereby forming back pressure. The increase in back pressure will affect the normal operation of the liquid chromatograph, causing the flow rate in the liquid chromatograph to slow down or even be interrupted. At this time, the vent valve opens the valve to discharge the increased liquid from the liquid chromatography system to reduce the back pressure accumulated on the stationary phase, so that the liquid chromatograph can resume normal operation.

[0061] In addition, if the vent valve is an automatic vent valve, the automatic vent valve will open at a certain time interval to discharge the deposits accumulated on the valve structure in the liquid chromatograph from the valve, thereby avoiding valve knotting and blockage problems in the valve structure in the liquid chromatograph.

[0062] ‌ Figure 2 A schematic diagram of a complete liquid extraction stroke of an existing liquid chromatograph is shown in FIG. Figure 2 The periodic scale points in the complete liquid extraction stroke schematic diagram of a liquid chromatograph provided in an embodiment of the present application are used to indicate that one cycle of the infusion pump of the liquid chromatograph is subdivided into multiple small cycles, and the starting point and end point of each small cycle are used to characterize the cycle position, wherein one cycle of the infusion pump of the liquid chromatograph is used to indicate the comprehensive liquid extraction process and liquid discharge process of the infusion pump of the liquid chromatograph.

[0063] Optionally, the pumping stroke is used to indicate the entire pumping stroke of the infusion pump, and the plunger displacement increment is used to indicate the running distance corresponding to each scale point in front of the plunger. Among them, a positive value is used to indicate that the plunger moves forward, that is, the infusion pump pushes the liquid forward; a negative value is used to indicate that the plunger moves backward, that is, the infusion pump pumps the liquid backward.

[0064] Optionally, the plunger is a key component of the solenoid valve. The function of the plunger is to control the flow of the fluid through the action of electromagnetic force. The electromagnet generates electromagnetic force through the current, so that the plunger moves up or down, thereby controlling the flow of the fluid. Among them, the structure of the plunger includes: an upper plunger, a lower plunger, an elastic sealing ring, a guide rod and other components. The elastic sealing ring seals the gap between the plunger and the magnetic core to ensure the sealing performance of the solenoid valve; the guide rod is used to guide the movement trajectory of the plunger, so that the movement process of the plunger is stable and reliable. It is worth noting that the movement speed of the plunger is affected by the current flowing through the solenoid valve, the magnetic permeability of the magnetic core, the quality of the plunger, and the resistance of the external medium.

[0065] Optionally, the dotted line position is used to indicate the switching action of the solenoid valve under ideal conditions, that is, under ideal conditions, when the period scale point corresponding to the dotted line position is reached, the solenoid valve is immediately actuated, the actuation time is 0, and the solvent in each channel is accurately distributed. However, the actual switching of the flow phase is achieved by the switching of the solenoid valve in conjunction with the specific flow path design. For example, when channel A switches to channel B, the solenoid valve of channel A is closed, and the solenoid valve of channel B is opened at the same time. During the switching process of channel A switching to channel B, the infusion pump continues to operate. The time factors and consistency of the opening and closing of the solenoid valves of channel A and channel B will have a certain impact on the accuracy and repeatability of the solvent ratio.

[0066] The multi-cycle distribution method of the liquid-phase low-pressure gradient pump provided in the embodiment of the present application is explained in detail below with reference to the accompanying drawings.

[0067] Figure 3 This is a flow chart of a multi-cycle distribution method of a liquid phase low-pressure gradient pump provided by this application. This method can be applied to computer equipment, third-party servers, terminal equipment, etc., and this application does not make specific limitations on this. Figure 3 The present application embodiment provides a liquid phase low-pressure gradient pump multi-cycle distribution method, comprising:

[0068] S301. Obtain the operating status of an infusion pump in a liquid chromatograph, and determine whether the liquid chromatograph meets the cycle conditions at the current moment according to the operating status.

[0069] Optionally, the operating status of the infusion pump is specifically used to indicate the current action of the infusion pump in the liquid chromatograph, such as whether the infusion pump is in the end of liquid extraction state, the preparation state before liquid extraction, the liquid extraction operation state, etc.

[0070] Optionally, according to the running state of the infusion pump in the liquid chromatograph, it is determined whether the liquid chromatograph meets the cycle division condition at the current moment, that is, according to the running state of the infusion pump in the liquid chromatograph, it is determined whether the infusion pump in the liquid chromatograph is in the preparation stage before liquid extraction at the current moment. That is, when the infusion pump in the liquid chromatograph is in the preparation stage before liquid extraction at the current moment, it is determined that the liquid chromatograph meets the cycle division condition at the current moment.

[0071] It is worth noting that the multi-cycle distribution method of the liquid-phase low-pressure gradient pump provided in the embodiment of the present application is mainly used in the liquid extraction stroke of the liquid chromatograph.

[0072] Optionally, the weekly condition is used to indicate whether the liquid chromatograph is about to enter a liquid extraction stroke. If the liquid chromatograph is in the process of weekly liquid extraction or the liquid chromatograph has completed liquid extraction, the liquid chromatograph does not meet the weekly condition at the current moment.

[0073] Optionally, the entire liquid extraction stroke of the liquid chromatograph is regarded as a large cycle. If the liquid extraction stroke of the liquid chromatograph is analyzed from the time dimension, the 0 time scale point is regarded as the pre-extraction preparation node of the liquid chromatograph. Whether the liquid chromatograph meets the cycle conditions at the current moment is judged based on whether the operating status of the liquid chromatograph at the current moment reaches the 0 time point.

[0074] It is worth noting that if the liquid chromatograph reaches time point 0 at the current moment, it can be determined that the liquid chromatograph has restarted a liquid extraction stroke or the liquid chromatograph has initially started a liquid extraction stroke, and this application does not make specific limitations on this.

[0075] S302: If yes, determine the actual number of cycles according to the target solvent ratio in the target sample solution, the flow rate of the infusion pump, the preset reference cycle factor, the number of cycles corresponding to the preset reference cycle factor, and the preset upper limit of the number of cycles.

[0076] Optionally, the target sample solution is used to indicate a sample solution that the user wants to obtain, and the target sample solution includes a plurality of solvents in the same proportion or in different proportions, and the target sample solution is obtained by fully mixing these solvents through a liquid chromatograph.

[0077] Optionally, the target solvent proportion in the target sample solution is used to indicate the proportion of the solvent with a smaller proportion among the solvents required for the target sample solution in the target sample solution. For example, the solvents required for the target sample solution A are: solvent a, solvent b, and solvent c. The proportion of solvent a in the target sample solution A is 5%, the proportion of solvent b in the target sample solution A is 40%, and the proportion of solvent c in the target sample solution A is 55%. The target solvent is solution a, and the target solvent proportion is 5%. The above is only an example and the present application does not make any specific limitation on this.

[0078] Optionally, the flow rate of the infusion pump is used to indicate the operating speed of the infusion pump in the liquid chromatograph to extract the solvent required for the target sample solution under the drive of the drive motor. The operating flow rate of the infusion pump is jointly determined by the displacement increment of the main cam and the displacement increment of the auxiliary cam in the liquid chromatograph, and this application does not make specific limitations on this.

[0079] Optionally, the preset reference frequency factor is a reference frequency factor pre-set by the user according to the cam design of the infusion pump in the liquid chromatograph, the target sample solution volume obtained by the post-stage mixing, etc., that is, the preset reference frequency factor can be regarded as the reference frequency factor of the current frequency, wherein the cam design includes the stroke of the cam, and the preset reference frequency factor can be 0.2, 0.3, 0.5, etc., and the present application does not make specific restrictions on this; the frequency number corresponding to the preset reference frequency factor is the frequency number pre-set by the user according to the preset reference frequency factor, and the frequency number corresponding to the preset reference frequency factor can be 2 weeks, 3 weeks, 5 weeks, etc., and the present application does not make specific restrictions on this. It is worth noting that the actual frequency number can be proportionally calculated with reference to the frequency number corresponding to the preset reference frequency factor.

[0080] Exemplarily, the reference division factor preset by the user is 0.3, and the number of divisions corresponding to the preset reference division factor can be set to 3 weeks.

[0081] Optionally, the preset upper limit of the number of cycles is a threshold value of the number of cycles pre-set by the user according to the working performance of the liquid chromatograph, and the preset upper limit of the number of cycles may be 5 weeks, 10 weeks, etc., and this application does not make specific restrictions on this. Among them, when the actual number of cycles exceeds the preset upper limit of the number of cycles, it may cause the solvent extracted by the infusion pump to be segmented, thereby causing the liquid chromatograph to be unable to fully mix the target sample solution.

[0082] It is worth noting that the larger the proportion of solvent in the target sample solution, the lower the mixing requirement of the solvent, and this application does not make any specific limitation on this.

[0083] Optionally, the actual number of cycles is used to indicate the actual number of cycles of the liquid extraction stroke of the liquid chromatograph, and the actual number of cycles can be 2 cycles, 3 cycles, 5 cycles, 10 cycles, etc., which is not specifically limited in this application. Among them, the actual number of cycles can only be less than or equal to the preset upper limit of the cycle.

[0084] Optionally, the actual number of cycles is calculated based on the target solvent ratio in the target sample solution, the flow rate of the infusion pump in the liquid chromatograph, a preset reference cycle factor, the number of cycles corresponding to the preset reference cycle factor, and a preset upper limit of the number of cycles.

[0085] Optionally, the liquid extraction stroke of the liquid chromatograph is divided into cycles according to the actual number of cycles to obtain multiple small-cycle liquid extraction strokes. The starting time point and the ending time point of each small cycle after division can be determined according to the period scale points of the liquid extraction stroke of the divided small cycles.

[0086] S303: Determine whether the liquid chromatograph is currently in an allocation cycle according to the operating status of the liquid chromatograph and the actual number of divided cycles.

[0087] Optionally, the allocation cycle is used to indicate the liquid extraction allocation cycle of the infusion pump in the liquid chromatograph to extract the solvent with the smallest proportion in the target sample solution. It is worth noting that the allocation cycle is the liquid extraction allocation cycle set for the solvent with the smallest or smaller proportion in the target sample solution, that is, the solvent with the smallest proportion required by the target sample solution is extracted in place at one time within the allocation cycle.

[0088] Optionally, the smaller the proportion of solvent required in the target sample solution, the shorter the extraction time required for the solvent with a small proportion under the same channel flow rate. If the solvent with a small proportion is extracted in batches, the extraction time for each extraction of the solvent may be very short. If there is an opening and closing error in the channel valve where the solvent is located, it will seriously affect the extraction accuracy of the solvent.

[0089] Optionally, the liquid chromatograph's pumping stroke is divided into cycles based on the actual number of divided cycles, and based on the division results of the liquid chromatograph's pumping stroke and the operating status of the liquid chromatograph at the current moment, it is determined whether the liquid chromatograph has reached the allocation cycle at the current operating moment.

[0090] Optionally, the allocation cycle is set to the first pumping cycle in the pumping stroke division result of the liquid chromatograph, that is, when the infusion pump in the liquid chromatograph starts to pump liquid, the solvent with a small proportion required by the target sample solution is extracted in one go within the first pumping cycle.

[0091] Exemplarily, the volume of the target sample solution A is 100 mL, and the proportion of solvent a required for the target sample solution A is 5%, that is, the volume of solvent a is 5 mL. When the liquid chromatograph reaches the allocation week on the time scale, 5 mL of solvent a is completely extracted once in the entire allocation week, and solvent a is no longer extracted in the subsequent non-allocation week.

[0092] S304: If yes, determine the allocation week ratio corresponding to the allocation week according to the target solvent ratio in the target sample solution and the actual number of divided weeks, and extract each solvent required for the target sample solution within the allocation week according to the allocation week ratio.

[0093] Optionally, the allocation week ratio is used to indicate the ratio of the target solvent required in a smaller proportion in the target sample solution within the allocation week and the target solvent extracted by the infusion pump within the allocation week. Based on the ratio corresponding to the target solvent required in a smaller proportion in the target sample solution and the calculated actual number of divided weeks, the allocation week ratio corresponding to the target solvent required in a smaller proportion in the target sample solution in the allocation week is determined.

[0094] Optionally, if it is determined that the liquid chromatograph reaches the allocation week in the time dimension, each solvent required in the target sample solution is extracted within the allocation week according to the allocation week ratio.

[0095] In an embodiment of the present application, by acquiring the operating state of the liquid chromatograph, and determining the operating state of the infusion pump in the liquid chromatograph according to the operating state of the liquid chromatograph, it is judged whether the liquid chromatograph meets the cycle conditions at the current moment according to the operating state of the infusion pump in the liquid chromatograph; if so, the actual cycle number is calculated according to the target solvent ratio required for the target sample solution, the flow rate of the infusion pump in the liquid chromatograph, the preset reference cycle factor, the number of cycles corresponding to the preset reference cycle factor, and the preset upper limit of the number of cycles; the entire liquid extraction stroke of the liquid chromatograph is processed in cycles based on the actual cycle number to obtain the liquid extraction cycle result of the liquid chromatograph, and it is judged whether the liquid chromatograph has reached the allocation cycle according to the liquid extraction cycle result of the liquid chromatograph and the operating state of the liquid chromatograph; if so, the allocation cycle ratio corresponding to the allocation cycle is calculated according to the target solvent ratio required for the target sample solution and the actual cycle number, so as to control the infusion pump in the liquid chromatograph to extract each solvent required for the target sample solution within the allocation cycle according to the allocation cycle ratio. Among them, the entire extraction stroke of the liquid chromatograph is converted into the time dimension by introducing a division factor, and the actual division number is calculated according to the solvent ratio of the target solvent with a smaller proportion required by the target sample solution and the preset division factor and other reference parameters. The entire extraction stroke of the liquid chromatograph is divided into periods based on the actual division number, and each solvent required by the target sample solution is drawn according to the distribution week ratio within the distribution week, so that the target solvent with a smaller proportion of solvent is fully extracted within the distribution week, so as to extend the single extraction time of the target solvent, thereby reducing the switching error of the channel valve where the target solvent with a smaller proportion of solvent is located, and improving the extraction accuracy of the target solvent. In this way, the accuracy and repeatability of the solvent ratio of small proportion or large flow rate can be optimized, thereby improving the accuracy and reliability of the liquid chromatograph.

[0096] In an optional embodiment, see Figure 4 The operation of step S302 may specifically be:

[0097] S401. Determine a current cycle factor according to the target solvent ratio in the target sample solution and the flow rate of the infusion pump.

[0098] Optionally, the current cycle factor is used to indicate the cycle factor of the liquid chromatograph's pumping stroke at the current moment, wherein the current cycle factor is calculated based on the solution ratio corresponding to the target solvent with a smaller solvent ratio in the target sample solution and the current flow rate of the infusion pump in the liquid chromatograph.

[0099] S402: Determine a theoretical number of divided weeks according to a current divided week factor, a preset reference divided week factor, and a number of divided weeks corresponding to the preset reference divided week factor.

[0100] Optionally, the theoretical sub-week number is used to indicate the theoretical sub-week value corresponding to the current sub-week factor, that is, the theoretical sub-week number corresponding to the current sub-week factor can be calculated based on the current sub-week factor, a preset reference sub-week factor and the sub-week number corresponding to the preset reference sub-week factor.

[0101] S403: Determine the actual number of divided weeks according to the result of rounding down the theoretical number of divided weeks and the preset upper limit of the number of divided weeks.

[0102] Optionally, the actual number of weeks is used to indicate the number of weeks that can be implemented in practice corresponding to the current number of weeks factor. The actual number of weeks corresponding to the current number of weeks factor is determined based on the comparison result between the rounded-down result of the theoretical number of weeks and a preset upper limit of the number of weeks.

[0103] In an optional implementation manner, the operation of step S401 may specifically be:

[0104] Calculating a first product between a target solvent ratio in a target sample solution and a preset threshold value;

[0105] calculating a first ratio between the flow rate of the infusion pump and the first product;

[0106] The current cycle factor is determined according to the first ratio.

[0107] Optionally, the current cycle factor can be calculated according to the following formula (1), which is as follows:

[0108] C = V ÷ (P × 100) (1)

[0109] Optionally, C is used to represent the current cycle factor, V is used to represent the flow rate of the infusion pump in the liquid chromatograph at the current moment, and P is used to indicate the target solvent ratio required for the target sample solution.

[0110] For example, if the flow rate V of the infusion pump in the liquid chromatograph at the current moment is 1.0 mL / min, and the proportion P of the target solvent a required by the target sample solution A in the target sample solution A is 0.5%, then the current cycle factor C at the current moment is 1.0÷(0.5%×100)=2.

[0111] In an optional implementation manner, the operation of step S402 may specifically be:

[0112] Calculating a second ratio between the current clocking factor and a preset reference clocking factor;

[0113] Calculating a second product between the second ratio and the number of division cycles corresponding to a preset reference division cycle factor;

[0114] Based on the second product, determine the theoretical number of sub-cycles.

[0115] Optionally, the theoretical number of sub-weeks can be calculated according to the following formula (2):

[0116] M=C÷n×N(2)

[0117] Optionally, M is used to represent the theoretical number of weeks, C is used to represent the current week factor, n is used to represent a preset reference week factor, and N is used to represent the number of weeks corresponding to the preset reference week factor.

[0118] For example, if the preset reference division factor n=0.3 at the current moment, the number of divisions N corresponding to the preset reference division factor=3, and the current division factor C=2, then the theoretical number of divisions M at the current moment=2÷0.3×3=20.

[0119] Exemplarily, if the reference division factor n=0.3 preset at the current moment, the division number N=3 corresponding to the preset reference division factor, the proportion of target solvent a required for target sample solution A in target sample solution A P=6%, and the flow rate of the infusion pump in the liquid chromatograph V=1.0mL / min, then the current division factor C=1.0÷(6%×100)=0.166666666667, the theoretical division number M=0.166666666667÷0.3×3=1.66666666667, then the result of rounding down the theoretical division number is 1.

[0120] In an optional embodiment, see Figure 5 The operation of step S403 may specifically be:

[0121] S501. If the result of rounding down the theoretical divided week number is greater than the preset divided week number upper limit, determine that the actual divided week number is the preset divided week number upper limit.

[0122] Optionally, if the result of rounding down the theoretical divided week number exceeds the limit of a preset upper limit of the divided week number, the actual divided week number is the preset upper limit of the divided week number.

[0123] For example, if the result of rounding down the theoretical number of cycles at the current moment is 20, and the preset upper limit of the number of cycles is 10, then the actual number of cycles of the liquid chromatograph at the current moment is 10.

[0124] S502: If the rounded-down result of the theoretical divided week number is less than or equal to the preset upper limit of the divided week number, the actual divided week number is determined to be the rounded-down result of the theoretical divided week number.

[0125] Optionally, if the result of rounding down the theoretical divided week number does not exceed the preset upper limit of the divided week number, the actual divided week number is the result of rounding down the theoretical divided week number.

[0126] For example, if the result of rounding down the theoretical number of cycles at the current moment is 10, and the preset upper limit of the number of cycles is 10, then the actual number of cycles of the liquid chromatograph at the current moment is 10; if the result of rounding down the theoretical number of cycles at the current moment is 1, and the preset upper limit of the number of cycles is 10, then the actual number of cycles of the liquid chromatograph at the current moment is 1.

[0127] In an optional implementation manner, the operation of step S304 may specifically be:

[0128] Calculate the third product between the target solvent ratio in the target sample solution and the actual number of divided cycles;

[0129] Based on the third product, the allocation week ratio corresponding to the allocation week is determined.

[0130] Optionally, the allocation week ratio corresponding to the allocation week can be calculated according to the following formula (3), and the formula (3) is as follows:

[0131] F = P × Q (3)

[0132] Optionally, F is used to indicate the distribution week ratio of the target solvent required by the target sample solution in the distribution week, Q is used to indicate the actual number of distribution weeks, and P is used to indicate the proportion of the target solvent required by the target sample solution.

[0133] For example, if the solvent proportion of target solvent a required by target sample solution A at the current moment is P=0.5%, and the actual number of divided weeks Q=10, then the distribution week proportion F corresponding to the target solvent a required by target sample solution A in the distribution week is F=0.5%×10=5%.

[0134] In an optional implementation manner, the operation of step S303 further includes:

[0135] If the liquid chromatograph is not currently in a dispensing cycle, the extraction of the target solvent is stopped.

[0136] Optionally, if it is determined according to the operating state of the liquid chromatograph at the current moment that the liquid chromatograph has not reached the allocation cycle at the current moment, the extraction of the target solvent required for the target sample solution is stopped.

[0137] In an optional embodiment, see Figure 6 The multi-cycle distribution method of the liquid phase low-pressure gradient pump provided in the embodiment of the present application also includes:

[0138] S601 . Determine a channel flow rate corresponding to a target solvent in a target sample solution according to a target solvent ratio in a target sample solution and a flow rate of an infusion pump.

[0139] Optionally, the channel flow rate is used to indicate the liquid flow rate in the channel where the target solvent required by the target sample solution is located. According to the target solvent ratio required by the target sample solution and the flow rate of the infusion pump, the liquid flow rate of the target solvent required by the target sample solution in the channel through which the target solvent flows can be calculated.

[0140] S602 , determining the extraction time of the target solvent in the target sample solution according to the target solvent ratio in the target sample solution and the channel flow rate.

[0141] Optionally, the extraction time of the target solvent is used to indicate the extraction time required for the infusion pump in the liquid chromatograph to extract a smaller proportion of the target solvent at a time based on the multi-cycle allocation method. According to the proportion of the target solvent required by the target sample solution and the liquid flow rate of the target solvent required by the target sample solution in the channel where the target solvent is located, the extraction time corresponding to the target solvent required by the target sample solution can be calculated.

[0142] In an optional implementation manner, the operation of step S601 may specifically be:

[0143] calculating a fourth product between the flow rate of the infusion pump and the proportion of the target solvent in the target sample solution;

[0144] According to the fourth product, the channel flow rate of the target solvent in the target sample solution is determined.

[0145] Optionally, the channel flow rate corresponding to the target solvent required by the target sample solution can be calculated according to the following formula (4), and the formula (4) is as follows:

[0146] v = V × P (4)

[0147] Optionally, v is used to represent the channel flow rate of the target solvent required by the target sample solution in the channel through which the target solvent flows, V is used to represent the flow rate of the infusion pump in the liquid chromatograph, and P is used to indicate the proportion of the target solvent required by the target sample solution.

[0148] For example, if the flow rate of the infusion pump in the liquid chromatograph is 1.0 mL / min at the current moment, and the solvent proportion of the target solvent a required by the target sample solution A is P=0.5%, then the channel flow rate of the target solvent a required by the target sample solution A in the channel x where it is located is v=1.0 mL / min×0.5%=0.005 mL / min.

[0149] Figure 7 For a flow chart of a liquid phase low pressure gradient pump multi-cycle distribution method provided in this application, see Figure 7 The specific working process of the multi-cycle allocation method of the liquid phase low-pressure gradient pump provided in the embodiment of the present application is as follows: the liquid chromatograph starts to start, and according to the operating state of the infusion pump of the liquid chromatograph at the current time, the operating state of the infusion pump equivalent to the pumping stroke time dimension is determined to determine whether the liquid chromatograph runs to the cycle calculation position; if it is determined that the liquid chromatograph runs to the cycle calculation position at the current moment, the current cycle factor is calculated according to the target solvent proportion in the target sample solution and the flow rate of the infusion pump, and the theoretical cycle number is calculated according to the current cycle factor, the preset reference cycle factor and the preset reference cycle factor The corresponding cycle number, and the actual cycle number is determined according to the result of rounding down the theoretical cycle number and the preset cycle number upper limit The comparison result; the liquid chromatograph pumping stroke is divided into cycles based on the actual cycle number, and it is determined whether the liquid chromatograph runs to the allocation cycle. If so, the allocation cycle ratio corresponding to the allocation cycle is calculated according to the target solvent proportion in the target sample solution and the actual cycle number, and each solvent required for the target sample solution is extracted according to the allocation cycle ratio within the allocation cycle.

[0150] In an optional implementation, Figure 8 For the implementation effect of the liquid phase low pressure gradient pump multi-cycle distribution method provided in the embodiment of the present application, see Figure 8 The multi-cycle distribution method of the liquid-phase low-pressure gradient pump provided in the embodiment of the present application can extend the extraction time of the small-proportion solvent to reduce the influence of the switching error of the low-pressure gradient pump on the extraction accuracy of the small-proportion solvent.

[0151] For example, Figure 8 The preset reference cycle factor is 0.2, the corresponding cycle number is 2, the upper limit of the cycle is 5, the theoretical step length of the infusion pump to extract 1% solvent is x, and the time required for the infusion pump to move forward at a flow rate of 1.0mL / min is t milliseconds. Among them, the units of infusion flow rate and channel flow rate are mL / min, the units of channel ratio, distribution cycle ratio and the ratio of distribution cycle to the whole are all %, the unit of small proportion step is uStep, and the unit of small proportion time is ms.

[0152] Depend on Figure 8It can be seen that after the multi-cycle allocation algorithm, the extraction time of the small proportion solvent is greatly extended while ensuring the accuracy of the target sample solution, thereby reducing the influence of the gradient valve switching error on the extraction accuracy of the small proportion solvent.

[0153] The following is a description of the computer equipment and computer-readable storage medium used to execute the liquid-phase low-pressure gradient multi-cycle distribution method provided in this application. The specific implementation process and technical effects are described above and will not be repeated below.

[0154] Fig. 9 is a schematic diagram of the structure of a computer device provided in an embodiment of the present application. Fig. 9 The computer device includes: a memory 901 and a processor 902. The memory 901 stores a computer program that can be run on the processor 902. When the processor 902 executes the computer program, the steps in any of the above method embodiments are implemented.

[0155] An embodiment of the present application further provides a computer-readable storage medium, which stores a computer program. When the computer program is executed by a processor, the steps in the above-mentioned method embodiments can be implemented.

[0156] Optionally, the present application also provides a program product, such as a computer-readable storage medium, comprising a program, which, when executed by a processor, is used to execute any of the above-mentioned liquid-phase low-pressure gradient pump multi-cycle distribution method embodiments.

[0157] The above-mentioned integrated unit implemented in the form of a software functional unit can be stored in a computer-readable storage medium. The above-mentioned software functional unit is stored in a storage medium, including a number of instructions for a computer device (which can be a personal computer, a server, or a network device, etc.) or a processor (English: processor) to perform some steps of the methods of each embodiment of the present invention. The aforementioned storage medium includes: U disk, mobile hard disk, read-only memory (English: Read-Only Memory, abbreviated: ROM), random access memory (English: Random Access Memory, abbreviated: RAM), disk or optical disk and other media that can store program codes.

[0158] The above are only specific implementations of the present application, but the protection scope of the present application is not limited thereto. Any technician familiar with the technical field can easily think of changes or substitutions within the technical scope disclosed in the present application, which should be included in the protection scope of the present application. Therefore, the protection scope of the present application should be based on the protection scope of the claims.

[0159] The above description is only the preferred embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A liquid phase low pressure gradient pump multi-cycle distribution method, characterized in that: The method comprises: Acquire the running status of the infusion pump in the liquid chromatograph, and determine whether the liquid chromatograph meets the cycle condition at the current moment according to the running status; If yes, the actual number of cycles is determined according to the target solvent ratio in the target sample solution, the flow rate of the infusion pump, the preset reference cycle factor, the number of cycles corresponding to the preset reference cycle factor, and the preset upper limit of the number of cycles; Determining whether the liquid chromatograph is currently in an allocation cycle according to the operating state of the infusion pump and the actual number of divided cycles; If yes, then determine the allocation week ratio corresponding to the allocation week according to the target solvent ratio in the target sample solution and the actual number of divided weeks, and extract each solvent required for the target sample solution within the allocation week according to the allocation week ratio; The step of determining the allocation week ratio corresponding to the allocation week according to the target solvent ratio in the target sample solution and the actual number of divided weeks includes: Calculating a third product between the target solvent ratio in the target sample solution and the actual number of divided cycles; The allocation week ratio corresponding to the allocation week is determined according to the third product.

2. The multi-cycle distribution method of the liquid phase low-pressure gradient pump according to claim 1, characterized in that: The actual number of cycles is determined according to the target solvent ratio in the target sample solution, the flow rate of the infusion pump, the preset reference cycle factor, the number of cycles corresponding to the preset reference cycle factor, and the preset upper limit of the number of cycles, including: Determining a current cycle factor according to the target solvent ratio in the target sample solution and the flow rate of the infusion pump; Determine a theoretical number of divided weeks according to the current divided week factor, a preset reference divided week factor, and the number of divided weeks corresponding to the preset reference divided week factor; The actual number of weeks is determined based on the rounded-down result of the theoretical number of weeks and the preset upper limit of the number of weeks.

3. The multi-cycle distribution method of the liquid phase low-pressure gradient pump according to claim 2, characterized in that: Determining the current cycle factor according to the target solvent ratio in the target sample solution and the flow rate of the infusion pump includes: Calculating a first product between the target solvent ratio in the target sample solution and a preset threshold value; calculating a first ratio between the flow rate of the infusion pump and the first product; The current cycle factor is determined according to the first ratio.

4. The multi-cycle distribution method of the liquid phase low-pressure gradient pump according to claim 2, characterized in that: The determining of the theoretical number of divided weeks according to the current divided week factor, the preset reference divided week factor and the number of divided weeks corresponding to the preset reference divided week factor comprises: Calculating a second ratio between the current clock factor and the preset reference clock factor; Calculating a second product of the second ratio and the number of division cycles corresponding to the preset reference division cycle factor; Based on the second product, the theoretical number of divided cycles is determined.

5. The multi-cycle distribution method of the liquid phase low-pressure gradient pump according to claim 2, characterized in that: Determining the actual number of divided weeks according to the result of rounding down the theoretical number of divided weeks and the preset upper limit of the number of divided weeks includes: If the result of rounding down the theoretical divided week number is greater than the preset divided week number upper limit value, then determining the actual divided week number to be the preset divided week number upper limit value; If the rounded-down result of the theoretical divided week number is less than or equal to the preset divided week number upper limit, the actual divided week number is determined to be the rounded-down result of the theoretical divided week number.

6. The multi-cycle distribution method of the liquid phase low-pressure gradient pump according to claim 1, characterized in that: Also includes: If the liquid chromatograph is not currently in a dispensing cycle, the extraction of the target solvent is stopped.

7. The multi-cycle distribution method of a liquid phase low-pressure gradient pump according to claim 1, characterized in that: The method further comprises: Determining a channel flow rate corresponding to the target solvent in the target sample solution according to the target solvent ratio in the target sample solution and the flow rate of the infusion pump; The duration of extracting the target solvent from the target sample solution is determined according to the target solvent ratio in the target sample solution and the channel flow rate.

8. The multi-cycle distribution method of a liquid phase low-pressure gradient pump according to claim 1, characterized in that: The determining, according to the target solvent proportion in the target sample solution and the flow rate of the infusion pump, a channel flow rate corresponding to the target solvent in the target sample solution comprises: calculating a fourth product between the flow rate of the infusion pump and the target solvent proportion in the target sample solution; The channel flow rate corresponding to the target solvent in the target sample solution is determined according to the fourth product.

9. A computer device, characterized in that: include: A memory and a processor, wherein the memory stores a computer program that can be run on the processor, and when the processor executes the computer program, the steps of the method described in any one of claims 1 to 8 are implemented.

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