A salt-alkali-resistant, high-temperature-resistant and growth-promoting composition containing edodene, and its preparation method and application

Abstract

The present invention belongs to the field of agricultural technology, and relates to a salt-alkali-resistant, high temperature-resistant, growth-promoting composition containing ekdoin, and a preparation method and application thereof. The composition is made of the following components by weight: 12-15 parts of aloin, 6-10 parts of ekdoin, 10-15 parts of seaweed extract, 10-15 parts of γ-aminobutyric acid, 3-4 parts of polyvinyl alcohol, 2-3 parts of sodium dodecylbenzene sulfonate, 3-4 parts of Tween-20, 5-8 parts of glycerol, 5-8 parts of ethyl salicylate, 5-10 parts of chitosan oligosaccharides, and 45-50 parts of water. The composition of the present invention significantly improves the stress resistance of crops, alleviates the membrane damage of crops caused by saline-alkali and high temperature stress, enhances the activity of antioxidant enzymes in crop leaves under saline-alkali and high temperature stress, and promotes the growth of crops under saline-alkali and high temperature stress conditions.

Description

Technical Field

[0001] The invention belongs to the technical field of agriculture, and relates to a salt-alkali resistant, high temperature resistant and growth-promoting composition containing ectoine, a preparation method and an application thereof. Background Art

[0002] Saline-alkali land is the main type of medium- and low-yield land in my country. Soil salinization is an important factor limiting crop growth and production, and has become a resource constraint for the sustainable development of irrigation agriculture in the world. Salt-alkali stress can cause osmotic stress, hypoxia stress and ion poisoning, hinder plant photosynthesis, affect its water absorption, cause a large amount of active oxygen accumulation, and ultimately lead to plant tissue aging or death.

[0003] Due to the intense light intensity and high outside temperature in summer, the transpiration of crop water is large, and water cannot be replenished in time, resulting in crop burns. Especially in the hot and dry winds in the north, if the newly planted seedlings lack water, the branches and trunks will "cramp", the leaves will curl or fall off, and in severe cases, the seedlings will die. In winter and spring, the temperature difference between day and night is large, and the tissues in the crops are affected by the changes in heat and cold, and they will also be damaged and sunburn will occur. The main causes of sunburn are drought causing crop water shortage; long-term high temperature outside induces sunburn damage; the growth habits of crop species are affected by environmental factors, etc.

[0004] Ecdoin, also known as tetrahydromethylpyrimidine carboxylic acid, is an osmotic pressure protective substance widely found in halophilic bacteria and halophilic bacteria. As the main osmotic pressure compensation solute in most moderate halophilic bacteria, Ecdoin accumulates in high concentrations in cells to balance the external osmotic pressure. It is characterized by high water solubility, no static charge, and no active groups. Ecdoin is a natural cell protectant with stable properties.

[0005] It is still necessary to develop salt-alkali resistance, high temperature resistance and growth-promoting products to enhance the stress resistance of crops and increase yields. Summary of the invention

[0006] In view of this, the object of the present invention is to provide a salt-alkali resistant, high temperature resistant, growth-promoting composition containing ectoine, as well as a preparation method and application. The salt-alkali resistant, high temperature resistant, growth-promoting composition containing ectoine of the present invention significantly improves the stress resistance of crops, increases the plant height and leaf area of ​​crops under salt-alkali stress, reduces the content of REC and MDA, and increases the activity of POD, SOD, and CAT; increases the chlorophyll content of crops under high temperature stress, increases the yield of fresh fruit ears and grains, and promotes crop growth.

[0007] To achieve the above objectives, this application adopts the following technical solutions:

[0008] In the first aspect, the present invention provides a salt-alkali resistant, high temperature resistant and growth-promoting composition containing ectoine, which is made of the following components by weight: 12-15 parts of aloin, 6-10 parts of ectoine, 10-15 parts of seaweed extract, 10-15 parts of γ-aminobutyric acid, 3-4 parts of polyvinyl alcohol, 2-3 parts of sodium dodecylbenzene sulfonate, 3-4 parts of Tween-20, 5-8 parts of glycerol, 5-8 parts of ethyl salicylate, 5-10 parts of chitosan oligosaccharides and 45-50 parts of water.

[0009] Furthermore, the salt-alkali resistant, high temperature resistant and growth-promoting composition containing ectoine is made of the following components by weight: 15 parts of aloin, 8 parts of ectoine, 15 parts of seaweed extract, 12 parts of γ-aminobutyric acid, 4 parts of polyvinyl alcohol, 2 parts of sodium dodecylbenzene sulfonate, 3 parts of Tween-20, 6 parts of glycerol, 6 parts of ethyl salicylate, 8 parts of chitosan oligosaccharides and 45 parts of water.

[0010] Furthermore, the salt-alkali resistant, high temperature resistant and growth-promoting composition containing ectoine is made of the following components by weight: 12 parts of aloin, 10 parts of ectoine, 15 parts of seaweed extract, 10 parts of γ-aminobutyric acid, 4 parts of polyvinyl alcohol, 2 parts of sodium dodecylbenzene sulfonate, 3 parts of Tween-20, 5 parts of glycerol, 8 parts of ethyl salicylate, 10 parts of chitosan oligosaccharides and 50 parts of water.

[0011] Furthermore, the salt-alkali resistant, high temperature resistant and growth-promoting composition containing ectoine is made of the following components by weight: 15 parts of aloin, 6 parts of ectoine, 10 parts of seaweed extract, 15 parts of γ-aminobutyric acid, 3 parts of polyvinyl alcohol, 3 parts of sodium dodecylbenzene sulfonate, 4 parts of Tween-20, 8 parts of glycerol, 5 parts of ethyl salicylate, 5 parts of chitosan oligosaccharides and 45 parts of water.

[0012] Furthermore, the preparation method of the seaweed extract is: grinding the kelp dry, adding a composite enzyme for enzymolysis at a temperature of 50°C and a pH of 6.0, fully reacting for 72 hours to obtain an enzymolysis solution, filtering the enzymolysis solution to obtain a filtrate, and drying to obtain the seaweed extract.

[0013] Furthermore, the complex enzyme is composed of cellulase and pectinase in a mass ratio of 2:1; and the added amount of the complex enzyme is 1.4% of the dry weight of the kelp.

[0014] In a second aspect, the present invention provides a method for preparing the above-mentioned salt-alkali resistant, high temperature resistant, and growth-promoting composition containing ectoine, the preparation method being: (1) slowly adding seaweed extract into water, stirring evenly to prepare a solution; (2) heating to 50-60°C, weighing aloin and ectoine according to the weight parts of the formula composition, adding them to the above-mentioned solution, stirring while adding, to prepare a mixture; (3) weighing γ-aminobutyric acid, polyvinyl alcohol, sodium dodecylbenzene sulfonate, Tween-20, glycerol, ethyl salicylate, and chitosan oligosaccharide according to the weight parts of the formula composition, adding them to the above-mentioned mixture, mixing evenly, drying, and grinding through a 100-mesh sieve to obtain the target composition.

[0015] Furthermore, in step (2), the temperature is raised to 55°C.

[0016] In a third aspect, the present invention provides the use of the salt-alkali resistant, high temperature resistant, growth-promoting composition containing ectoine obtained by the above preparation method in promoting crop growth and / or sun protection.

[0017] In a fourth aspect, the present invention provides a method for using the salt-alkali resistant, high temperature resistant, growth-promoting composition containing ectoine, including: diluting the salt-alkali resistant, high temperature resistant, growth-promoting composition containing ectoine by 10-60 times, and spraying it on the leaves and / or fruit surfaces of the crops to be treated.

[0018] Furthermore, the salt-alkali resistant, high temperature resistant and growth-promoting composition containing ectoine is diluted 30 or 50 times and sprayed on the leaves and / or fruit surfaces of the crops to be treated.

[0019] Beneficial effects achieved by the present invention:

[0020] The salt-alkali resistance, high temperature resistance and growth-promoting composition containing ekdoin of the present invention significantly improves the stress resistance of crops, increases the plant height and leaf area of ​​crops under salt-alkali stress, reduces the content of REC and MDA, and increases the activities of POD, SOD and CAT; increases the chlorophyll content of crops under high temperature stress, and increases the yield of fresh fruit ears and grains. The composition of the present invention can alleviate the membrane damage of crops caused by salt-alkali and high temperature stress, enhance the activity of antioxidant enzymes in the leaves of crops under salt-alkali and high temperature stress, and promote the growth of crops under salt-alkali and high temperature stress conditions. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the drawings required for the specific implementation methods are briefly introduced below.

[0022] Figure 1 Comparison of plant height among treatments

[0023] Compared with the XP group, * P<0.05, ** P<0.01; compared with group A,# P<0.05.

[0024] Figure 2 Comparison of leaf area among treatments

[0025] Compared with the XP group, * P<0.05, ** P<0.01; compared with group A, # P<0.05.

[0026] Figure 3 Comparison of REC among treatments

[0027] Compared with the XP group, * P<0.05, ** P<0.01; compared with group A, # P<0.05, ## P<0.01.

[0028] Figure 4 Comparison of MDA content among treatments

[0029] Compared with the XP group, * P<0.05, ** P<0.01; compared with group A, # P<0.05.

[0030] Figure 5 Comparison of POD activity among treatments

[0031] Compared with the XP group, * P<0.05, ** P<0.01; compared with group A, # P<0.05.

[0032] Figure 6 Comparison of SOD activity among treatments

[0033] Compared with the XP group, * P<0.05, ** P<0.01; compared with group A, # P<0.05, ## P<0.01.

[0034] Figure 7 Comparison of CAT activity among treatments

[0035] Compared with the XP group, * P<0.05, ** P<0.01; compared with group A, # P<0.05.

[0036] Figure 8 Comparison of chlorophyll content

[0037] Compared with the CK group, * P<0.05, ** P<0.01; compared with group A, # P<0.05, ## P<0.01.

[0038] Fig. 9 Comparison of ear weight per plant

[0039] Compared with the CK group, * P<0.05, ** P<0.01; compared with group A, # P<0.05, ## P<0.01.

[0040] Fig.10 Comparison of fresh kernel quality

[0041] Compared with the CK group, * P<0.05, ** P<0.01; compared with group A, # P<0.05, ## P<0.01.

[0042] Fig.11 Comparison of dry kernel quality

[0043] Compared with the CK group, * P<0.05, ** P<0.01; compared with group A, # P<0.05, ## P<0.01. DETAILED DESCRIPTION

[0044] It should be understood that the terms described in the present invention are only for describing special embodiments and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0045] The drugs required for the present invention are conventional experimental drugs, which are purchased from commercial channels; the experimental methods not mentioned are conventional experimental methods and will not be described in detail here.

[0046] The preparation method of the seaweed extract in the following Examples 1-6 and Comparative Examples 1-3 is as follows: grind the kelp dry, add a composite enzyme for enzymolysis at a temperature of 50°C and pH=6.0, fully react for 72 hours to obtain an enzymolysis solution, filter the enzymolysis solution to obtain a filtrate, dry it, and obtain the seaweed extract. The composite enzyme is composed of cellulase and pectinase in a mass ratio of 2:1; the amount of the composite enzyme added is 1.4% of the dry weight of the kelp.

[0047] Example 1

[0048] 15 parts of aloesin, 8 parts of ectoine, 15 parts of seaweed extract, 12 parts of gamma-aminobutyric acid, 4 parts of polyvinyl alcohol, 2 parts of sodium dodecylbenzene sulfonate, 3 parts of Tween-20, 6 parts of glycerol, 6 parts of ethyl salicylate, 8 parts of chitosan oligosaccharide and 45 parts of water.

[0049] The preparation method is as follows: (1) slowly adding seaweed extract into water, stirring evenly to prepare a solution; (2) heating to 55°C, weighing aloin and ectoine according to the weight of the formula composition, adding them to the above solution, stirring while adding, to prepare a mixture; (3) weighing γ-aminobutyric acid, polyvinyl alcohol, sodium dodecylbenzene sulfonate, Tween-20, glycerol, ethyl salicylate, and chitosan oligosaccharide according to the weight of the formula composition, adding them to the above mixture, mixing evenly, drying, and grinding through a 100-mesh sieve to obtain the target composition.

[0050] Example 2

[0051] 12 parts of aloesin, 10 parts of ectoine, 15 parts of seaweed extract, 10 parts of gamma-aminobutyric acid, 4 parts of polyvinyl alcohol, 2 parts of sodium dodecylbenzene sulfonate, 3 parts of Tween-20, 5 parts of glycerol, 8 parts of ethyl salicylate, 10 parts of chitosan oligosaccharides and 50 parts of water.

[0052] The preparation method is as follows: (1) slowly adding seaweed extract into water and stirring evenly to prepare a solution; (2) heating to 50°C, weighing aloin and ectoine according to the weight of the formula composition, adding them to the above solution, stirring while adding, to prepare a mixture; (3) weighing γ-aminobutyric acid, polyvinyl alcohol, sodium dodecylbenzene sulfonate, Tween-20, glycerol, ethyl salicylate, and chitosan oligosaccharide according to the weight of the formula composition, adding them to the above mixture, mixing evenly, drying, and grinding through a 100-mesh sieve to obtain the target composition.

[0053] Example 3

[0054] 15 parts of aloesin, 6 parts of ectoine, 10 parts of seaweed extract, 15 parts of gamma-aminobutyric acid, 3 parts of polyvinyl alcohol, 3 parts of sodium dodecylbenzene sulfonate, 4 parts of Tween-20, 8 parts of glycerol, 5 parts of ethyl salicylate, 5 parts of chitosan oligosaccharide and 45 parts of water.

[0055] The preparation method is as follows: (1) slowly adding seaweed extract into water and stirring evenly to prepare a solution; (2) heating to 60°C, weighing aloin and ectoine according to the weight of the formula composition, adding them to the above solution, stirring while adding, to prepare a mixture; (3) weighing γ-aminobutyric acid, polyvinyl alcohol, sodium dodecylbenzene sulfonate, Tween-20, glycerol, ethyl salicylate, and chitosan oligosaccharide according to the weight of the formula composition, adding them to the above mixture, mixing evenly, drying, and grinding through a 100-mesh sieve to obtain the target composition.

[0056] Example 4

[0057] 12 parts of aloesin, 8 parts of ectoine, 10 parts of seaweed extract, 15 parts of gamma-aminobutyric acid, 4 parts of polyvinyl alcohol, 2 parts of sodium dodecylbenzene sulfonate, 4 parts of Tween-20, 5 parts of glycerol, 8 parts of ethyl salicylate, 10 parts of chitosan oligosaccharides and 50 parts of water.

[0058] The preparation method is as follows: (1) slowly adding seaweed extract into water, stirring evenly to prepare a solution; (2) heating to 55°C, weighing aloin and ectoine according to the weight of the formula composition, adding them to the above solution, stirring while adding, to prepare a mixture; (3) weighing γ-aminobutyric acid, polyvinyl alcohol, sodium dodecylbenzene sulfonate, Tween-20, glycerol, ethyl salicylate, and chitosan oligosaccharide according to the weight of the formula composition, adding them to the above mixture, mixing evenly, drying, and grinding through a 100-mesh sieve to obtain the target composition.

[0059] Example 5

[0060] 15 parts of aloin, 10 parts of ectoine, 15 parts of seaweed extract, 10 parts of gamma-aminobutyric acid, 3 parts of polyvinyl alcohol, 3 parts of sodium dodecylbenzene sulfonate, 3 parts of Tween-20, 8 parts of glycerol, 5 parts of ethyl salicylate, 5 parts of chitosan oligosaccharide and 45 parts of water.

[0061] The preparation method is as follows: (1) slowly adding seaweed extract into water and stirring evenly to prepare a solution; (2) heating to 50°C, weighing aloin and ectoine according to the weight of the formula composition, adding them to the above solution, stirring while adding, to prepare a mixture; (3) weighing γ-aminobutyric acid, polyvinyl alcohol, sodium dodecylbenzene sulfonate, Tween-20, glycerol, ethyl salicylate, and chitosan oligosaccharide according to the weight of the formula composition, adding them to the above mixture, mixing evenly, drying, and grinding through a 100-mesh sieve to obtain the target composition.

[0062] Example 6

[0063] 13 parts of aloesin, 9 parts of ectoine, 12 parts of seaweed extract, 12 parts of gamma-aminobutyric acid, 3 parts of polyvinyl alcohol, 3 parts of sodium dodecylbenzene sulfonate, 4 parts of Tween-20, 6 parts of glycerol, 6 parts of ethyl salicylate, 9 parts of chitosan oligosaccharide and 48 parts of water.

[0064] The preparation method is as follows: (1) slowly adding seaweed extract into water and stirring evenly to prepare a solution; (2) heating to 60°C, weighing aloin and ectoine according to the weight of the formula composition, adding them to the above solution, stirring while adding, to prepare a mixture; (3) weighing γ-aminobutyric acid, polyvinyl alcohol, sodium dodecylbenzene sulfonate, Tween-20, glycerol, ethyl salicylate, and chitosan oligosaccharide according to the weight of the formula composition, adding them to the above mixture, mixing evenly, drying, and grinding through a 100-mesh sieve to obtain the target composition.

[0065] Comparative Example 1

[0066] 18 parts of aloesin, 17 parts of seaweed extract, 15 parts of gamma-aminobutyric acid, 4 parts of polyvinyl alcohol, 2 parts of sodium dodecylbenzene sulfonate, 3 parts of Tween-20, 6 parts of glycerol, 6 parts of ethyl salicylate, 8 parts of chitosan oligosaccharide and 45 parts of water.

[0067] Compared with Example 1, the difference is that ectoine is not contained, and the dosage of aloin, seaweed extract and γ-aminobutyric acid is different.

[0068] The preparation method is similar to that of Example 1.

[0069] Comparative Example 2

[0070] 13 parts of ectoine, 20 parts of seaweed extract, 17 parts of gamma-aminobutyric acid, 4 parts of polyvinyl alcohol, 2 parts of sodium dodecylbenzene sulfonate, 3 parts of Tween-20, 6 parts of glycerol, 6 parts of ethyl salicylate, 8 parts of chitosan oligosaccharide and 45 parts of water.

[0071] Compared with Example 1, the difference is that aloin is not contained, and the dosage of ectoine, seaweed extract and γ-aminobutyric acid is different.

[0072] The preparation method is similar to that of Example 1.

[0073] Comparative Example 3

[0074] 20 parts of aloesin, 13 parts of ectoine, 17 parts of gamma-aminobutyric acid, 4 parts of polyvinyl alcohol, 2 parts of sodium dodecylbenzene sulfonate, 3 parts of Tween-20, 6 parts of glycerol, 6 parts of ethyl salicylate, 8 parts of chitosan oligosaccharide and 45 parts of water.

[0075] Compared with Example 1, the difference is that the seaweed extract is not contained, and the dosage of aloin, ectoine and γ-aminobutyric acid is different.

[0076] The preparation method is similar to that of Example 1.

[0077] Comparative Example 4

[0078] 23 parts of aloesin, 15 parts of seaweed extract, 12 parts of gamma-aminobutyric acid, 4 parts of polyvinyl alcohol, 2 parts of sodium dodecylbenzene sulfonate, 3 parts of Tween-20, 6 parts of glycerol, 6 parts of ethyl salicylate, 8 parts of chitosan oligosaccharide and 45 parts of water.

[0079] Compared with Example 1, the difference is that ectoine is not contained and the amount of aloin used is different.

[0080] The preparation method is similar to that of Example 1.

[0081] Comparative Example 5

[0082] 23 parts of ectoine, 15 parts of seaweed extract, 12 parts of gamma-aminobutyric acid, 4 parts of polyvinyl alcohol, 2 parts of sodium dodecylbenzene sulfonate, 3 parts of Tween-20, 6 parts of glycerol, 6 parts of ethyl salicylate, 8 parts of chitosan oligosaccharide and 45 parts of water.

[0083] Compared with Example 1, the difference is that aloin is not contained and the dosage of ectoine is different.

[0084] The preparation method is similar to that of Example 1.

[0085] Comparative Example 6

[0086] 15 parts of aloesin, 8 parts of ectoine, 27 parts of seaweed extract, 4 parts of polyvinyl alcohol, 2 parts of sodium dodecylbenzene sulfonate, 3 parts of Tween-20, 6 parts of glycerol, 6 parts of ethyl salicylate, 8 parts of chitosan oligosaccharide and 45 parts of water.

[0087] Compared with Example 1, the difference is that γ-aminobutyric acid is not contained and the amount of seaweed extract used is different.

[0088] The preparation method is similar to that of Example 1.

[0089] Test Example 1 Effect of the composition of the present invention on the growth of kidney bean seedlings under saline-alkali stress

[0090] 1. Experimental Methods

[0091] Kidney beans were used as the test material. The seeds were disinfected with 1% sodium hypochlorite for 8 minutes, then rinsed with clean water several times, rinsed with deionized water 3 times, and finally soaked in water for 12 hours. After that, they were placed in a culture dish covered with moist filter paper and waited for 48 hours to germinate. The germinated seeds with consistent growth were selected and planted in a flower pot (8cm×8cm) filled with vermiculite and perlite as the matrix, where the weight ratio of vermiculite to perlite was 3:1, and then placed in an artificial climate box for cultivation at 25℃ / 18℃ (day / night), photoperiod of 16h / 8h (light / dark), and light intensity of 300μmol / (m 2 ·s) and cultured at a relative humidity of 60%.

[0092] At the three-leaf and one-heart stage, uniformly growing kidney bean seedlings were selected for treatment. Seven treatments were set up: (1) blank control group (CK group): watering with clean water; (2) saline-alkali stress group (XP group): 100mmol / L saline-alkali stress treatment (50mmol / LNaCl+50mmol / LNaHCO3 solution)+watering with clean water; (3) Example 1 group (A group): saline-alkali stress+composition of Example 1; (4) Example 2 group (B group): saline-alkali stress+composition of Example 2; (5) Comparative Example 1 group (C group): saline-alkali stress+composition of Comparative Example 1; (6) Comparative Example 2 group (D group): saline-alkali stress+composition of Comparative Example 2; (7) Comparative Example 3 group (E group): saline-alkali stress+composition of Comparative Example 3. Three replicates were set up for each treatment.

[0093] The products of Example 1, Example 2, Comparative Example 1, Comparative Example 2, and Comparative Example 3 were diluted 50 times respectively. At 6 pm every day, each treatment was sprayed with clean water, Example 1, Example 2, Comparative Example 1, Comparative Example 2, and Comparative Example 3 products on the leaves until water droplets dripped from the leaves, and the treatment was continued for 6 days. Each treatment had 6 pots, and each pot had 2 plants. Samples were taken after 6 days of treatment, and the leaves of the seedlings were treated with liquid nitrogen and stored in a -80°C refrigerator for measuring various physiological indicators.

[0094] 2. Determination of indicators

[0095] 2.1 Plant height and leaf area

[0096] Plant height was measured by extending a string from the base of the seedling stem to the top growing point.

[0097] The total leaf area of ​​individual plants was measured using a leaf area scanner, and each treatment was replicated three times.

[0098] 2.2 Physiological indicators

[0099] The leaf samples stored in -80℃ refrigerator were taken out to measure the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), malondialdehyde (MDA) content and relative conductivity (REC). Each treatment was repeated 3 times when measuring the indicators.

[0100] 3. Data processing

[0101] Graphpad Prism7.0 software and SPSS19.0 data processing system were used for data statistical analysis and graphing.

[0102] 4. Test results

[0103] 4.1 Effects on plant height and leaf area

[0104] like Figure 1 , 2As shown in the figure, compared with the CK group, the plant height and leaf area of ​​the seedlings in the XP group were significantly reduced. Compared with the XP group, the plant height and leaf area were significantly increased in the A and B groups. The effect of the A group on increasing plant height and leaf area was the most obvious, with an increase of 103.85% and 86.08% in plant height and leaf area, respectively. The effect of the B group on increasing plant height and leaf area was also very significant, with an increase of 90.31% and 77.28% in plant height and leaf area, respectively. The effect of the A and B groups on increasing plant height and leaf area was significantly better than that of the C, D and E groups.

[0105] The composition of the invention can alleviate the inhibitory effect of saline-alkali stress on the growth of kidney bean seedlings.

[0106] 4.2 Effect on REC and MDA content

[0107] like Figure 3 , 4 As shown in the figure, compared with the CK group, the REC and MDA contents of the seedlings in the XP group were significantly increased. Compared with the XP group, the treatments in groups A and B significantly reduced the REC and MDA contents. The REC and MDA contents in group A were reduced by 69.67% and 44.29%, respectively, and those in group B were reduced by 66.01% and 37.34%, respectively. The effects of groups A and B in reducing the REC and MDA contents were significantly better than those in groups C, D, and E.

[0108] The composition of the invention can alleviate the membrane damage of kidney bean seedling leaves caused by saline-alkali stress.

[0109] 4.3 Effects on antioxidant enzyme activity

[0110] like Figure 5 , 6 As shown in Figures 7 and 8, compared with the CK group, the activities of antioxidant enzymes POD, SOD, and CAT in the seedlings of the XP group were significantly increased. Compared with the XP group, the treatments of groups A and B further increased the activities of antioxidant enzymes POD, SOD, and CAT. The effects of groups A and B on increasing the activities of antioxidant enzymes POD, SOD, and CAT were significantly better than those of groups C, D, and E.

[0111] Kidney bean plants have their own oxidative stress protection mechanism under saline-alkali stress, which protects cells from oxidative damage by activating the activity of antioxidant enzymes. The composition of the present invention can further induce and enhance the activity of antioxidant enzymes in leaves under saline-alkali stress.

[0112] Test Example 2 Effect of the composition of the present invention on photosynthetic characteristics and yield of corn under high temperature stress

[0113] 1. Experimental Methods

[0114] The test was conducted in the field. The test corn variety was Ludan 510. Six treatments were set up in the test: (1) Control group (CK group): high temperature stress + spraying water; (2) Example 1 group (A group): high temperature stress + Example 1 composition; (3) Example 3 group (B group): high temperature stress + Example 3 composition; (4) Comparative example 4 group (C group): high temperature stress + Comparative example 4 composition; (5) Comparative example 5 group (D group): high temperature stress + Comparative example 5 composition; (6) Comparative example 6 group (E group): high temperature stress + Comparative example 6 composition.

[0115] The products of Example 1, Example 3, Comparative Example 4, Comparative Example 5, and Comparative Example 6 were diluted 30 times respectively. 6 days before high temperature treatment, at 4 pm every day, the leaves of each treatment were sprayed with clean water, Example 1, Example 3, Comparative Example 4, Comparative Example 5, and Comparative Example 6, and sprayed every other day for 3 consecutive times. The spraying standard was that the droplets were evenly distributed on the surface of most leaves of corn. The area of ​​the test plot was 16m 2 (4m×4m), with 3 replicates for each treatment.

[0116] 5 days after spraying pretreatment, the field was treated with sheds and film covering to increase temperature, ensuring that the temperature in the shed was controlled between (37±2)℃ during the day. When the temperature was higher than 40℃, the two ends of the shed were opened for ventilation and cooling. The high temperature stress treatment lasted until the end of the filling period (15 days after treatment). Other field management was the same as conventional field production.

[0117] 2. Determination of indicators

[0118] 2.1 Chlorophyll content

[0119] 5 days after high temperature treatment, sample the same part of leaves from 5 ears in each treatment, weigh 0.1 g of sample, extract with 20 mL of 95% ethanol until the leaves completely lose their green color, measure the absorbance at wavelengths of 663 and 645 nm with an ultraviolet spectrophotometer, and calculate the chlorophyll a+b content using the following formula.

[0120] Chlorophyll a+b content (mg / g) = (20.2D645nm+8.02D663nm)×V / (1000×m).

[0121] Where: D663nm and D645nm are the absorbances at the measurement wavelengths; V is the volume of the extract, mL; m is the fresh weight of the sampled leaves, g.

[0122] 2.2 Output

[0123] At the milky stage, five representative ears were selected from each plot for testing, and the single-ear weight, single-plant grain fresh weight and single-plant dry weight were measured indoors.

[0124] 3. Data processing

[0125] Graphpad Prism7.0 software and SPSS19.0 data processing system were used for data statistical analysis and graphing.

[0126] 4. Test results

[0127] 4.1 Effect on chlorophyll content

[0128] like Figure 8 As shown, compared with the CK group, the treatments of group A and group B significantly increased the chlorophyll content. The effect of group A on increasing the chlorophyll content was the most obvious, with the chlorophyll content increasing by 29.70%. The effect of group B on increasing the chlorophyll content was also very significant, with the chlorophyll content increasing by 22.77%. The effect of group A and group B on increasing the chlorophyll content was significantly better than that of group C, group D, and group E.

[0129] The composition of the invention can increase the chlorophyll content of corn under high temperature stress conditions.

[0130] 4.2 Impact on the quality of individual ear

[0131] like Fig. 9 As shown, compared with the CK group, the treatments of group A and group B significantly increased the ear weight per plant. The ear weight per plant in group A increased by 29.09%, and the ear weight per plant in group B increased by 18.20%. The effect of group A and group B in increasing the ear weight per plant was significantly better than that of group C, group D, and group E.

[0132] The composition of the invention can improve the single-plant ear quality of corn under high temperature stress conditions.

[0133] 4.3 Impact on production

[0134] like Fig.10 , 11 As shown, compared with the CK group, the treatments of group A and group B significantly increased the weight of fresh grains and dry grains. The weight of fresh grains and dry grains in group A increased by 8.45% and 13.57%, respectively, and the weight of fresh grains and dry grains in group B increased by 6.83% and 13.22%, respectively. The effect of increasing the weight of fresh grains and dry grains in groups A and B was significantly better than that in groups C, D, and E.

[0135] The composition of the invention can improve the quality of fresh kernels and dry kernels of corn under high temperature stress conditions.

[0136] The above is only an embodiment of the present invention, and the common knowledge such as the known specific technical solutions and / or characteristics in the solution is not described in detail here. It should be pointed out that for those skilled in the art, without departing from the technical solution of the present invention, several modifications and improvements can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. A salt-alkali resistant, high temperature resistant, growth-promoting composition containing ectoine, characterized in that: The salt-alkali-resistant, high-temperature-resistant and growth-promoting composition containing ectoine is prepared from the following components by weight: 12-15 parts of aloin, 6-10 parts of ectoine, 10-15 parts of seaweed extract, 10-15 parts of gamma-aminobutyric acid, 3-4 parts of polyvinyl alcohol, 2-3 parts of sodium dodecylbenzene sulfonate, 3-4 parts of Tween-20, 5-8 parts of glycerol, 5-8 parts of ethyl salicylate, 5-10 parts of chitosan oligosaccharide and 45-50 parts of water; The preparation method of the seaweed extract comprises: grinding dried kelp, adding a composite enzyme for enzymolysis at a temperature of 50° C. and a pH of 6.0, reacting for 72 hours to obtain an enzymolysis solution, filtering the enzymolysis solution to obtain a filtrate, and drying to obtain the seaweed extract; The complex enzyme is composed of cellulase and pectinase in a mass ratio of 2:1; and the added amount of the complex enzyme is 1.4% of the dry weight of the kelp.

2. The salt-alkali-resistant, high-temperature-resistant, growth-promoting composition containing ectoine according to claim 1, characterized in that: The salt-alkali resistant, high temperature resistant and growth-promoting composition containing ectoine is made of the following components by weight: 15 parts of aloin, 8 parts of ectoine, 15 parts of seaweed extract, 12 parts of gamma-aminobutyric acid, 4 parts of polyvinyl alcohol, 2 parts of sodium dodecylbenzene sulfonate, 3 parts of Tween-20, 6 parts of glycerol, 6 parts of ethyl salicylate, 8 parts of chitosan oligosaccharide and 45 parts of water.

3. The salt-alkali-resistant, high-temperature-resistant, growth-promoting composition containing ectoine according to claim 1, characterized in that: The salt-alkali resistant, high temperature resistant and growth-promoting composition containing ectoine is made of the following components by weight: 12 parts of aloin, 10 parts of ectoine, 15 parts of seaweed extract, 10 parts of gamma-aminobutyric acid, 4 parts of polyvinyl alcohol, 2 parts of sodium dodecylbenzene sulfonate, 3 parts of Tween-20, 5 parts of glycerol, 8 parts of ethyl salicylate, 10 parts of chitosan oligosaccharides and 50 parts of water.

4. The salt-alkali-resistant, high-temperature-resistant, growth-promoting composition containing ectoine according to claim 1, characterized in that: The salt-alkali resistant, high temperature resistant and growth-promoting composition containing ectoine is made of the following components by weight: 15 parts of aloin, 6 parts of ectoine, 10 parts of seaweed extract, 15 parts of gamma-aminobutyric acid, 3 parts of polyvinyl alcohol, 3 parts of sodium dodecylbenzene sulfonate, 4 parts of Tween-20, 8 parts of glycerol, 5 parts of ethyl salicylate, 5 parts of chitosan oligosaccharide and 45 parts of water.

5. The salt-alkali-resistant, high-temperature-resistant, growth-promoting composition containing ectoine according to claim 1, characterized in that: The preparation method of the composition is as follows: (1) slowly adding seaweed extract into water, stirring evenly to prepare a solution; (2) heating to 50-60° C., weighing aloin and ectoine according to the weight of the formula composition, adding them to the above solution, stirring while adding, to prepare a mixture; (3) weighing γ-aminobutyric acid, polyvinyl alcohol, sodium dodecylbenzene sulfonate, Tween-20, glycerol, ethyl salicylate, and chitosan oligosaccharide according to the weight of the formula composition, adding them to the above mixture, mixing evenly, drying, and grinding through a 100-mesh sieve to obtain the target composition.

6. The salt-alkali-resistant, high-temperature-resistant, growth-promoting composition containing ectoine according to claim 5, characterized in that: In the step (2), the temperature is raised to 55°C.

7. An application of the salt-alkali-resistant, high-temperature-resistant, growth-promoting composition containing ectoine according to claim 1 in promoting crop growth and / or sun protection.

8. The use according to claim 7, characterized in that: The method for using the salt-alkali resistant, high temperature resistant and growth-promoting composition containing ectoine comprises: diluting the salt-alkali resistant, high temperature resistant and growth-promoting composition containing ectoine by 10-60 times and spraying it on the leaves and / or fruit surfaces of the crops to be treated.

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