Composition for improving skin state, functional hydrogel dressing and application of functional hydrogel dressing

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a composition for improving the skin state, a functional hydrogel dressing and application of the functional hydrogel dressing. The composition for improving the skin condition, provided by the invention, is prepared by taking the asiaticoside, the coptis chinensis extracting solution and the purslane extracting solution as core components, and matching the core components with the nicotinamide, the allantoin, the ceramide and the sodium hyaluronate. The composition provided by the invention achieves the effects of repairing the skin barrier, improving the skin state and adjusting the skin color through multiple mechanisms of resisting skin inflammation, resisting oxidation, inhibiting tyrosinase activity and the like. The composition is applied to the functional hydrogel dressing, so that the antibacterial activity and the skin repairing capacity of the composition can be further improved, the use frequency can be reduced, and the composition has a wide development prospect.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to a composition capable of improving skin condition, a functional hydrogel dressing and application thereof in skin care products. Background Art

[0002] Inflammation and skin sensitivity are common chronic recurrent inflammatory skin problems, with symptoms including severe itching and recurrent skin damage, which greatly reduce the patient's quality of life and physical and mental health. Therefore, the development of new skin care products that are easy to use in daily life is of great significance for the repair and relief of inflammatory and allergic skin problems.

[0003] Patent document CN101732205A discloses a skin care product composition that soothes allergic skin, resists inflammation, and resists irritation, wherein the composition contains one or more of grape seed extract, cactus extract, and dipotassium glycyrrhizinate. The raw materials of the composition are all soothing natural ingredients, the raw materials are natural and mild, and have little irritation to the skin. It can resist inflammation, soothe allergies, repair and protect the skin through multiple pathways, and improve skin immunity. It is easily accepted by consumers and has good application and market prospects.

[0004] Patent document CN115089521A discloses a soothing anti-inflammatory composition. The soothing anti-inflammatory composition includes sodium carboxymethyl β-glucan, purslane extract, Centella asiatica extract, sea fennel extract and panthenol. By using these five ingredients in combination, they can effectively exert their respective effects, alleviate skin sensitivity from multiple dimensions, and effectively soothe sensitive skin; at the same time, they can also be added as functional components into soothing anti-inflammatory cosmetics, and the obtained soothing anti-inflammatory cosmetics also show excellent skin repair ability; and the preparation method of the soothing anti-inflammatory composition and soothing anti-inflammatory cosmetics provided is simple, which is conducive to practical application.

[0005] Patent document CN117959217A discloses a natural plant extract, which includes the following components: Rehmannia root extract, Coptis root extract, Phellodendron bark extract, Curcuma root extract, Angelica root extract, and modified Purslane extract. The plant extract uses modified tea tree oil and adds modified Purslane to improve the symptoms of desquamation in patients with non-infectious inflammation, and can alleviate the pain symptoms of skin pollen allergy, and has good effects in skin anti-allergy and anti-inflammatory aspects.

[0006] However, the anti-inflammatory and antioxidant skin care products on the market are often too single-minded. They may have a certain effect in the short term, but long-term use will cause the skin to develop tolerance and rebound, and the effect will deteriorate. Therefore, researching and developing an efficient, safe, and economical anti-inflammatory, antioxidant, and skin color regulating skin care product is still a problem that needs to be solved urgently. Summary of the invention

[0007] In view of the above shortcomings, the present invention provides a composition for improving skin condition and a functional hydrogel dressing. The composition for improving skin condition provided by the present invention is prepared with total glycosides of Centella asiatica, extract of Coptis chinensis and extract of Portulaca oleracea as core ingredients, and is matched with niacinamide, allantoin, ceramide and sodium hyaluronate. The various components of the composition for improving skin condition synergistically have significant effects of anti-skin inflammation, repairing skin barrier, improving skin condition and regulating skin color. At the same time, the application of the composition in a functional hydrogel dressing can not only improve the antibacterial property and skin repair ability of the composition, but also reduce the frequency of use, and has good application value.

[0008] In order to achieve the above technical objectives, the technical solution provided by the present invention is as follows:

[0009] The present invention provides a composition for improving skin condition, comprising the following components in parts by weight:

[0010] 2-15 parts of niacinamide, 0.1-2 parts of allantoin, 0.5-8 parts of total Centella asiatica glycosides, 2-10 parts of Coptis chinensis extract, 1-8 parts of Portulaca oleracea extract, 1-5 parts of ceramide, 0.5-7 parts of sodium hyaluronate, add water to 100 parts.

[0011] Furthermore, the weight ratio of the Centella asiatica total glucoside, the Coptis chinensis extract and the Portulaca oleracea extract is (5-8):(5-10):(3-7).

[0012] Furthermore, the weight ratio of the Centella asiatica total glucoside, the Coptis chinensis extract and the Portulaca oleracea extract is 6.4:8.9:6.3.

[0013] Furthermore, the present invention provides a method for preparing the composition for improving skin condition, and the specific steps are as follows:

[0014] Mix niacinamide, allantoin, ceramide and water evenly, and stir for 5 to 15 minutes at a temperature of 30 to 40°C; then add total glucosides of Centella asiatica, coptis extract and purslane extract, and stir for 5 to 15 minutes at a temperature of 30 to 40°C; then add sodium hyaluronate, and stir for 1 to 10 minutes at a temperature of 30 to 40°C; cool to room temperature to obtain the product.

[0015] In addition, the present invention also provides a functional hydrogel dressing, which comprises the above composition for improving skin condition and a thermosensitive hydrogel carrier.

[0016] Furthermore, the temperature-sensitive hydrogel carrier is chitosan and β-glycerophosphate disodium hydrate.

[0017] Furthermore, the present invention also provides a method for preparing the functional hydrogel dressing, comprising the following steps:

[0018] Step S1, dissolving chitosan in a citric acid aqueous solution with a mass concentration of 1%, stirring until the chitosan is completely dissolved, and preparing a chitosan solution with a mass concentration of 4-6%;

[0019] Step S2, dissolving β-glycerophosphate disodium hydrate in deionized water to prepare a β-glycerophosphate disodium hydrate solution with a mass concentration of 1 to 2%;

[0020] Step S3, mixing the chitosan solution obtained in step S1 with the β-glycerophosphate disodium hydrate obtained in step S2 to obtain a mixed solution, adding the above composition for improving skin condition to the mixed solution, stirring evenly to obtain an aqueous phase;

[0021] Step S4, mixing the liquid paraffin and the emulsifier evenly to obtain an oil phase;

[0022] Step S5, mixing the water phase obtained in step S3 and the oil phase obtained in step S4 at a temperature of 35-40° C., stirring and emulsifying, and then adding glycerol dropwise, stirring for 28-35 minutes for cross-linking reaction to obtain the product.

[0023] Furthermore, in step S3, the volume ratio of the chitosan solution to the β-glycerophosphate disodium hydrate solution is 1:2, and the volume ratio of the mixed solution to the composition for improving skin condition is (1-2):2.

[0024] Furthermore, the emulsifier in step S4 is composed of Tween 80 and Span 80 mixed in a volume ratio of 5:2.

[0025] In addition, the present invention also claims to protect the use of the composition for improving skin condition or the functional hydrogel dressing in the preparation of skin care products.

[0026] Furthermore, the skin care products are anti-inflammatory and repair skin care products, and the composition for improving skin condition or the functional hydrogel dressing is added in an amount of 5 to 50% by mass in the skin care products. The anti-inflammatory, antioxidant and skin color regulating skin care products are skin care water, facial masks, gels or creams.

[0027] The composition provided by the present invention is composed of seven components, including nicotinamide, allantoin, ceramide, asiatica total glycosides, coptis extract, purslane extract, sodium hyaluronate, etc. In the composition, asiatica total glycosides, coptis extract and purslane extract are core components, which mainly play the role of anti-inflammatory, anti-oxidation and skin repair. Centella asiatica total glycosides have the effects of calming and soothing, and promoting skin cell regeneration, coptis extract has the effect of inhibiting the growth and reproduction of bacteria and viruses on damaged skin, and purslane extract can alleviate the symptoms of redness, swelling, itching and bacterial infection of damaged skin. The inventor has found through experiments that the anti-inflammatory and antioxidant effects of the composition can be significantly increased by combining the three in a specific ratio.

[0028] In addition, the inventors found that mixing niacinamide, allantoin, ceramide and sodium hyaluronate in a specific ratio can synergistically promote skin cell regeneration and wound healing, enhance skin barrier function, reduce water loss, and improve the skin's moisturizing ability. At the same time, niacinamide can inhibit the transfer of melanin to epidermal cells, reduce pigmentation, and help regulate skin color.

[0029] The composition provided by the present invention is prepared with Centella asiatica total glucosides, Coptis chinensis extract and Portulaca oleracea extract as core ingredients, and is combined with niacinamide, allantoin, ceramide and sodium hyaluronate. The ingredients interact with each other to have the effects of anti-skin inflammation, anti-oxidation, repairing skin barrier and regulating skin color, and can fundamentally improve skin condition.

[0030] Furthermore, in order to make the composition for improving skin condition have the effect of better improving skin condition and regulating skin color, the composition is further preferably composed of the following ingredients:

[0031] 10-15 parts of niacinamide, 0.3-0.5 parts of allantoin, 0.5-2 parts of Centella asiatica total glucosides, 3-7 parts of Coptis chinensis extract, 1-3 parts of Portulaca oleracea extract, 1-2 parts of ceramide, 1-3 parts of sodium hyaluronate, add water to 100 parts.

[0032] Furthermore, in order to make the composition for improving skin condition have the effect of better improving damaged skin and repairing skin barrier, the composition is further preferably composed of:

[0033] 2-5 parts of niacinamide, 1-2 parts of allantoin, 2-5 parts of Centella asiatica total glucosides, 2-5 parts of Coptis chinensis extract, 1-3 parts of Portulaca oleracea extract, 3-5 parts of ceramide, 5-7 parts of sodium hyaluronate, add water to 100 parts.

[0034] Furthermore, in order to make the composition for improving skin condition have better anti-inflammatory and antioxidant effects, the composition is preferably composed of:

[0035] 3-7 parts of niacinamide, 0.3-1 parts of allantoin, 5-8 parts of Centella asiatica total glucosides, 5-10 parts of Coptis chinensis extract, 3-7 parts of Portulaca oleracea extract, 1-2 parts of ceramide, 2-5 parts of sodium hyaluronate, add water to 100 parts.

[0036] In summary, compared with the prior art, the composition for improving skin condition provided by the present invention can achieve the effects of repairing skin barrier, improving skin condition, and regulating skin color through multi-layer mechanisms such as anti-skin inflammation, anti-oxidation, and inhibition of tyrosinase activity. The composition is applied to functional hydrogel dressings to further improve the antibacterial property and skin repair ability of the composition. At the same time, the adhesion and sustained release of the hydrogel dressing can also reduce the frequency of use, and has broad development prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 This is a graph showing the results of measuring the DPPH radical scavenging rate of the compositions for improving skin condition prepared in Examples 1 to 3.

[0038] Figure 2 This is a graph showing the measurement results of changes in tyrosinase activity of the compositions for improving skin condition prepared in Examples 1 to 3.

[0039] Figure 3 The graphs show the results of HE staining of mice in each group 10 days after administration.

[0040] Figure 4 The graph shows the changes in the levels of SOD, MDA, 1L-6, 1L-1β and TNF-α in the skin tissues of mice in each group.

[0041] Figure 5 This is a comparison of the differential expression of genes in the skin of each group of mice.

[0042] Figure 6 This is a graph showing the results of GSEA pathway enrichment analysis of Example 6 and the AD group. DETAILED DESCRIPTION

[0043] The present invention is further described below by the description of specific embodiments, but this is not a limitation of the present invention. Those skilled in the art can make various modifications or improvements based on the basic idea of ​​the present invention, but as long as they do not deviate from the basic idea of ​​the present invention, they are all within the scope of the present invention. The raw materials involved in the present invention can be obtained by commercially available or conventional technical means in the field. Among them: the brand name of Centella asiatica total glycosides is Yuanye, the product number is V10743, the brand name of Coptis chinensis extract is Luo En, the product number is 141433-60-5, and the brand name of Portulaca oleracea extract is Luo En, the product number is RH-02099.

[0044] Example 1: A composition for improving skin condition

[0045] The composition for improving skin condition is composed of the following ingredients and their weight parts:

[0046] 12.9 parts of niacinamide, 0.5 parts of allantoin, 1.2 parts of Centella asiatica total glycosides, 5.7 parts of Coptis chinensis extract, 2.5 parts of Portulaca oleracea extract, 1.8 parts of ceramide, 2.2 parts of sodium hyaluronate, add water to 100 parts;

[0047] The preparation process of the composition for improving skin condition is as follows:

[0048] Add niacinamide, allantoin, ceramide and water into a stirring tank, heat the stirring tank to 33°C, and stir for 12 minutes; maintain the temperature of the stirring tank at 33°C, then add total glucosides of Centella asiatica, Coptis chinensis extract and Portulaca oleracea extract, and stir for 12 minutes; maintain the temperature of the stirring tank at 33°C, then add sodium hyaluronate, stir for 6 minutes, and finally reduce the temperature of the stirring tank to room temperature.

[0049] Example 2: A composition for improving skin condition

[0050] The composition for improving skin condition is composed of the following ingredients and their weight parts:

[0051] 4.5 parts of niacinamide, 0.7 parts of allantoin, 6.4 parts of total Centella asiatica glycosides, 8.9 parts of Coptis chinensis extract, 6.3 parts of Portulaca oleracea extract, 1.7 parts of ceramide, 3.5 parts of sodium hyaluronate, add water to 100 parts;

[0052] The preparation process of the composition for improving skin condition is as follows:

[0053] Add niacinamide, allantoin, ceramide and water into a stirring tank, heat the stirring tank to 35°C, and stir for 10 minutes; maintain the temperature of the stirring tank at 35°C, then add total glucosides of Centella asiatica, Coptis chinensis extract and Portulaca oleracea extract, and stir for 10 minutes; maintain the temperature of the stirring tank at 35°C, then add sodium hyaluronate, stir for 5 minutes, and finally reduce the temperature of the stirring tank to room temperature.

[0054] Example 3: A composition for improving skin condition

[0055] The composition for improving skin condition is composed of the following ingredients and their weight parts:

[0056] 4.5 parts of niacinamide, 1.5 parts of allantoin, 4.5 parts of Centella asiatica total glycosides, 3.2 parts of Coptis chinensis extract, 1.5 parts of Portulaca oleracea extract, 3.9 parts of ceramide, 6.7 parts of sodium hyaluronate, add water to 100 parts;

[0057] The preparation process of the composition for improving skin condition is as follows:

[0058] Add niacinamide, allantoin, ceramide and water into a stirring tank, heat the stirring tank to 38°C, and stir for 8 minutes; maintain the temperature of the stirring tank at 38°C, then add Centella asiatica total glucosides, Coptis chinensis extract, and Portulaca oleracea extract, and stir for 8 minutes; maintain the temperature of the stirring tank at 38°C, then add sodium hyaluronate, and stir for 4 minutes, and finally reduce the temperature of the stirring tank to room temperature.

[0059] Example 4: A functional hydrogel dressing

[0060] The functional hydrogel dressing comprises the composition for improving skin condition prepared in Example 1 and a thermosensitive hydrogel carrier, wherein the thermosensitive hydrogel carrier is chitosan and β-glycerophosphate disodium hydrate.

[0061] The preparation process of the functional hydrogel dressing is as follows:

[0062] Step S1, dissolving 1 g of chitosan in a citric acid aqueous solution with a mass concentration of 1%, stirring until the chitosan is completely dissolved, to prepare a chitosan solution with a mass concentration of 5%;

[0063] Step S2, dissolving 2 g of β-glycerophosphate disodium hydrate in deionized water to prepare a β-glycerophosphate disodium hydrate solution with a mass concentration of 1%;

[0064] Step S3, mixing the chitosan solution obtained in step S1 and the β-glycerophosphate disodium hydrate obtained in step S2 at a volume ratio of 1:2 to obtain a mixed solution, adding 20 mL of the composition for improving skin condition obtained in Example 1 to 17.3 mL of the mixed solution, stirring evenly to obtain an aqueous phase;

[0065] Step S4, uniformly mixing 10 mL of liquid paraffin with 0.7 mL of an emulsifier, wherein the emulsifier is composed of a mixture of 0.5 mL of Tween 80 and 0.2 mL of Span 80, to obtain an oil phase;

[0066] Step S5, mixing the water phase obtained in step S3 and the oil phase obtained in step S4 at a temperature of 38° C., stirring and emulsifying, then adding 2 mL of glycerol dropwise, stirring for 28 minutes for cross-linking reaction, to obtain the product.

[0067] Example 5: A functional hydrogel dressing

[0068] The functional hydrogel dressing comprises the composition for improving skin condition prepared in Example 3 and a thermosensitive hydrogel carrier, wherein the thermosensitive hydrogel carrier is chitosan and β-glycerophosphate disodium hydrate.

[0069] The preparation process of the functional hydrogel dressing is as follows:

[0070] Step S1, dissolving 1 g of chitosan in a citric acid aqueous solution with a mass concentration of 1%, stirring until the chitosan is completely dissolved, to prepare a chitosan solution with a mass concentration of 5%;

[0071] Step S2, dissolving 2 g of β-glycerophosphate disodium hydrate in deionized water to prepare a β-glycerophosphate disodium hydrate solution with a mass concentration of 1%;

[0072] Step S3, mixing the chitosan solution obtained in step S1 and the β-glycerophosphate disodium hydrate obtained in step S2 at a volume ratio of 1:2 to obtain a mixed solution, adding 20 mL of the composition for improving skin condition obtained in Example 3 to 17.3 mL of the mixed solution, stirring evenly to obtain an aqueous phase;

[0073] Step S4, uniformly mixing 10 mL of liquid paraffin with 0.7 mL of an emulsifier, wherein the emulsifier is composed of a mixture of 0.5 mL of Tween 80 and 0.2 mL of Span 80, to obtain an oil phase;

[0074] Step S5, mixing the water phase obtained in step S3 and the oil phase obtained in step S4 at a temperature of 37° C., stirring and emulsifying, then adding 2 mL of glycerol dropwise, stirring for 30 min for cross-linking reaction, to obtain the product.

[0075] Example 6: A functional hydrogel dressing

[0076] The functional hydrogel dressing comprises the composition for improving skin condition prepared in Example 2 and a thermosensitive hydrogel carrier, wherein the thermosensitive hydrogel carrier is chitosan and β-glycerophosphate disodium hydrate.

[0077] The preparation process of the functional hydrogel dressing is as follows:

[0078] Step S1, dissolving 1 g of chitosan in a citric acid aqueous solution with a mass concentration of 1%, stirring until the chitosan is completely dissolved, to prepare a chitosan solution with a mass concentration of 5%;

[0079] Step S2, dissolving 2 g of β-glycerophosphate disodium hydrate in deionized water to prepare a β-glycerophosphate disodium hydrate solution with a mass concentration of 1%;

[0080] Step S3, mixing the chitosan solution obtained in step S1 and the β-glycerophosphate disodium hydrate obtained in step S2 at a volume ratio of 1:2 to obtain a mixed solution, adding 20 mL of the composition for improving skin condition obtained in Example 2 to 17.3 mL of the mixed solution, stirring evenly to obtain an aqueous phase;

[0081] Step S4, uniformly mixing 10 mL of liquid paraffin with 0.7 mL of an emulsifier, wherein the emulsifier is composed of a mixture of 0.5 mL of Tween 80 and 0.2 mL of Span 80, to obtain an oil phase;

[0082] Step S5, mixing the water phase obtained in step S3 and the oil phase obtained in step S4 at a temperature of 37° C., stirring and emulsifying, then adding 2 mL of glycerol dropwise, stirring for 30 min for cross-linking reaction, to obtain the product.

[0083] Test Example 1: In vitro free radical scavenging performance test

[0084] 1. Test materials:

[0085] The composition for improving skin condition prepared in Example 1, Example 2 and Example 3 is a niacinamide solution.

[0086] 2. Test methods:

[0087] The ability of the composition for improving skin condition prepared in Example 1, Example 2 and Example 3 to remove DPPH (1,1-diphenyl-2-trinitrophenylhydrazine) was determined, and a nicotinamide solution with a mass concentration of 13% was used as a control. The specific determination method is: 4.0mL (0.02mg / mL) of DPPH anhydrous ethanol solution and 2.0mL of anhydrous ethanol dilution (3mg / mL) of each composition sample were added to a 10mL colorimetric tube in sequence, and then anhydrous ethanol was added to the scale, mixed and immediately measured the absorbance value (A) at a wavelength of 517nm with a 1cm colorimetric dish, and the absorbance value was recorded as Ai, and then the absorbance value was measured after being stored in a greenhouse away from light for 30min, and recorded as Aj. The control test was an ethanol solution with only DPPH added, and its absorbance value was recorded as Ac. The free radical scavenging rate was the average of three measurements.

[0088] The free radical scavenging rate (K) is calculated as follows: K (%) = [l·(Ai·Aj) / Ac]*100%

[0089] 3. Test results:

[0090] The test results are as follows Figure 1 shown.

[0091] Figure 1 The figure is a graph showing the results of the determination of the DPPH free radical scavenging rate of the compositions for improving skin condition prepared in Examples 1, 2 and 3. Figure 1 It can be seen that the compositions for improving skin condition prepared in Examples 1 to 3 all exhibited better in vitro antioxidant capacity than niacinamide solutions of the same concentration, among which the antioxidant capacity of Example 2 was particularly outstanding.

[0092] Test Example 2: Tyrosinase Activity Test

[0093] 1. Test materials:

[0094] The compositions for improving skin condition prepared in Example 1, Example 2 and Example 3 are niacinamide solutions with a mass concentration of 13%.

[0095] 2. Test methods:

[0096] The ability of the compositions for improving skin condition prepared in Examples 1, 2 and 3 to adjust skin color was evaluated by measuring tyrosinase activity by colorimetry. The specific measuring method is as follows: 100 μL of 2.5 mmol / L L-tyrosine solution, 75 μL of PBS, and 75 μL of samples of different concentrations (5, 10 and 20 mg / mL) (PBS for the control group) were added to each well of a 96-well plate, mixed evenly, incubated at 30°C in the dark for 15 minutes, and the absorbance was measured at 475 nm. The operation and calculation were performed according to the detailed steps and calculation formula of the kit. The average value of three measurements was taken.

[0097] 3. Test results:

[0098] The test results are as follows Figure 2 shown.

[0099] Figure 2 The following is a graph showing the results of measuring the changes in tyrosinase activity of the compositions for improving skin condition prepared in Examples 1, 2 and 3. Figure 2 It can be seen that the compositions for improving skin condition prepared in Examples 1 to 3 all exhibited better ability to inhibit melanin production than niacinamide solutions of the same concentration, among which the inhibitory ability of Example 1 was particularly outstanding.

[0100] Experimental Example 3: Determination of Antioxidant and Inflammatory Factors of Functional Hydrogel Dressing in Vivo

[0101] 1. Test materials:

[0102] Functional hydrogel dressings prepared in Example 4, Example 5 and Example 6.

[0103] 2. Test methods:

[0104] 2.1. Preparation and grouping of atopic dermatitis mouse model:

[0105] The in vivo efficacy of the functional hydrogel dressing of the present invention in combating skin inflammation, anti-oxidation, improving skin condition, and regulating skin color was verified by using an atopic dermatitis mouse model (AD). 50 6-week-old BALB / c mice were selected and randomly divided into a control group (10), an AD model group (10), Example 4 (10), Example 5 (10), and Example 6 (10) according to the random number table method. All mice were adaptively fed for 7 days. After 1 week, each group of mice was smeared with a depilatory cream on the back of the mouse, and washed off with warm water after about 1 minute. The area of ​​the depilatory area was 2*2cm. On the second day of depilation, except for the control group, the other groups were smeared with 0.5% dinitrochlorobenzene (DNCB) on the depilated skin on the back of the mouse, 100μg per mouse, and applied continuously for 7 days. The model was successfully established when the back skin of the mouse formed erythema, edema, scratches, scales, and epidermal exfoliation.

[0106] After the model was stabilized, except for the control group and AD model group, the other groups began to be given the corresponding test substances for 10 consecutive days. The recovery status was observed and recorded every day. After the end of the medication, tissue morphology, in vivo antioxidant and inflammatory factor tests were performed.

[0107] 2.2. Observation of skin tissue morphology of mice in each group:

[0108] After the mice were anesthetized and killed, the back skin of the mice was taken and placed in 4% paraformaldehyde. After dehydration, transparency, wax immersion, embedding, slicing, dewaxing, slicing, dewaxing, HE staining and other steps, the pathological changes of the local skin of the mice were observed by a microscope imaging system. Five slices were selected for each tissue, and five fields of view were observed in each slice. Epidermal necrosis and dermal inflammatory cell infiltration were observed under a microscope.

[0109] 2.3. Determination of the contents of SOD, MDA, 1L-6, 1L-1β and TNF-α in the skin tissues of mice in each group:

[0110] Prepare the application solution required for the test according to the instructions of the superoxide dismutase (T-SOD) kit, add each reagent in turn, mix evenly and react at room temperature for 10 minutes, and use an enzyme marker to measure the absorbance of each well at a wavelength of 550nm. Calculate the total SOD activity in the skin tissue according to the formula.

[0111] According to the instructions of the malondialdehyde (MDA) kit, the application liquid required for the test was prepared, and each reagent was added in turn. After mixing evenly, the centrifuge tube was tightly capped. A small hole was pierced on the cap of the centrifuge tube with a syringe, and the reaction was carried out in a 95°C water bath for 80 minutes. After taking it out, it was cooled in running water and then centrifuged at 4°C; 4000r / min; 10min. After the centrifugation, the upper supernatant was collected, and the absorbance of each well was measured at a wavelength of 532nm using an enzyme marker, and the MDA content was calculated according to the formula of the kit. 1L-6, 1L-1β and TNF-α were operated according to the requirements of the kit.

[0112] 2.4. Determination of differential gene expression in the skin of mice in each group:

[0113] RNA Use Fibrous Tissue Mini Kit (Qiagen) was used for extraction. RNA library was constructed using TruSeqmRNA Sample Prep Kit v2 (Illumina) and sequenced on HiSeq 4000 platform. Mini Kit (Qiagen) was used for extraction, following the manufacturer's instructions. RNA libraries were constructed using the TruSeqStrandedmRNALT Sample Prep Kit (Illumina) and sequenced on the NovaSeq 6000 system using the NovaSeq 6000S4 kit. The raw reads were preprocessed to remove low-quality and adapter sequences before analysis, and the processed reads were aligned to the Mus musculus (mm10) genome using HISATv2.1.0.94. The reference genome sequence and annotation data were downloaded from UCSC. Transcript assembly and abundance estimation were performed using StringTie. After alignment, StringTie v1.3.4d assembled the aligned reads into transcripts and estimated abundance, providing relative abundance estimates (read count values) of transcripts and genes in each sample. The DESeq2 package was used to analyze differentially expressed genes between groups AD and Examples 4 to 6. GSEA software was used to perform gene set enrichment analysis on differentially expressed genes (log2 fold change ≥ 1.5 or ≤ -1.5) between the skins of mice with different treatments, and the Gene Ontology: Biological pathway gene set used was from MSigDB.

[0114] 3. Test results:

[0115] 3.1. Skin tissue morphology of mice in each group Figure 3 As shown:

[0116] Figure 3The results of HE staining 10 days after administration show that there is no excessive keratinization in the skin of mice in the control group, the thickness of the epidermis is normal, and the junction between the epidermis and the dermis is wavy and regularly arranged in the dermis. The stratum corneum of the skin of mice in the AD model group is significantly thickened, which is a phenomenon of excessive proliferation of keratinocytes and partial loss of epidermis in the damaged area. The stratum corneum of the skin of mice in groups 4 to 6 of Examples of the present invention is thicker than that of the control group, and scabs, inflammatory cell infiltration under the epidermis and in the dermis can be seen, and dermal papillae are visible but less. Among them, the skin repair after modeling in Example 5 is particularly prominent, and the thickness of the epidermis is basically normal.

[0117] 3.2. The results of determination of SOD, MDA, 1L-6, 1L-1β and TNF-α contents in the skin tissues of mice in each group are as follows: Figure 4 As shown:

[0118] Figure 4 The following is a graph showing the changes in the levels of SOD, MDA, 1L-6, 1L-1β and TNF-α in the skin tissues of mice in each group:

[0119] (1) MDA, as the final product of lipid oxidation in biological membranes, can cross-link with proteins, nucleic acids and other substances to generate insoluble substances, thereby causing cell membrane damage and affecting the exchange of substances between cells. Figure 4 (a) It can be seen that compared with the control group, the MDA content in the AD model group increased significantly, which shows that inflammation can increase the MDA content in skin tissue, thereby causing skin damage. Compared with the AD model group, the MDA content in Example 4 to 6 groups tended to decrease.

[0120] (2) The SOD antioxidant enzyme system is an important component of the body's resistance to oxidative stress and is one of the important indicators for evaluating the body's antioxidant capacity. Figure 4 (b) It can be seen that compared with the control group, the SOD activity in the tissues of the AD model group mice was significantly decreased. Compared with the AD model group, the SOD activity in the skin tissues of the Example 4 to 6 groups was significantly increased, and the effect of Example 6 was particularly prominent.

[0121] (3) When the skin is damaged, it will promote the secretion of pro-inflammatory factors such as 1L-6, 1L-1β and tumor necrosis factor TNF-α in the epidermis and dermis. The production of these pro-inflammatory factors will damage mitochondria and increase the production of ROS; and the increase in ROS will further promote the production of pro-inflammatory factors, thus forming a positive feedback mechanism. Figure 4(c, d, e) It can be seen that compared with the control group, the content of 1L-6, 1L-1β and TNF-α in the skin tissue of the AD model group increased significantly, indicating that inflammatory damage can significantly increase the content of 1L-6, 1L-1β and TNF-α in the skin tissue. Compared with the AD model group, the content of 1L-6, 1L-1β and TNF-α in the skin of animals in Examples 4 to 6 decreased significantly, among which the effect of Example 6 was particularly prominent.

[0122] 3.3. Gene differential expression results in the skin of each group of mice Figure 5 and Figure 6 As shown:

[0123] Figure 5 The comparison diagram of gene differential expression in the skin of each group of mice is shown in Figure 2. Figure 6 This is a graph showing the results of GSEA pathway enrichment analysis of Example 6 and the AD group.

[0124] (1) From Figure 5 It can be seen that the expression of genes related to inflammatory response such as "Tnf", "Cxcl13", skin barrier related genes such as "Krt17", "Fgf10", "Tgm3", "Flg2", "Shh", and oxidative stress related genes such as "Sod1", "Nox1", "Tlr4", "Foxo3", and "Mcl1" increased in the AD group, while decreased in the Example 4 to 6 groups. This indicates that this example can significantly inhibit inflammation, skin barrier damage, and oxidative stress increase caused by AD, and the expression change of composition Example 6 is the most significant.

[0125] (2) From Figure 6 It can be seen that the results of GSEA pathway enrichment analysis show that compared with the AD group, the gene set related to oxidative stress and inflammatory response in Example 6 showed a significant down-regulation trend (as shown in Figures B and C), while the gene set related to skin protection showed a significant up-regulation trend (as shown in Figure D). This change in gene expression pattern indicates that Example 6 may have the effect of enhancing the skin protection function while reducing oxidative stress and inflammatory response, which is of great significance for skin health and disease treatment.

[0126] Test Example 5: Clinical Trial

[0127] (1) Testing the irritation of functional hydrogel dressings to human skin:

[0128] The functional hydrogel dressing prepared in Example 4 was used for the test, and a total of 50 subjects were recruited, including 15 males and 35 females, aged between 18 and 50 years old. The specific test method is: about 1.5*1.5cm gel, using the closed patch test method, the test object is fixed on the back of the subject with a low-allergenic tape, and the test object is removed after 24 hours. The skin reaction is observed at 0.5, 24, and 48 hours after removal, and the results are recorded according to the skin reaction grading standard in the "Safety Technical Specifications for Cosmetics" (2015 Edition). The results show that 0 cases of adverse skin reactions occurred in 50 people.

[0129] (2) Testing the efficacy of functional hydrogel dressings in improving skin condition and regulating skin color:

[0130] The functional hydrogel dressings prepared in Example 4, Example 5 and Example 6 were used for the test, and a total of 30 female subjects aged between 18 and 50 years old were recruited. In addition, this clinical trial selected a nicotinamide solution with a mass concentration of 4.5% instead of the composition and added it to the functional hydrogel as a positive control, and the negative control was not applied. A solar simulator was used to irradiate the skin of specific parts of the subjects. After the melanization test was completed, different dosing groups applied the corresponding dosing parts continuously for 4 weeks, once a day, and then before application (0 week) and in the 1st, 2nd, 3rd, and 4th weeks, respectively, and then used Miravex 3D skin imager to comprehensively analyze the changes in skin color. The test results are shown in Table 1:

[0131] Table 1 Clinical trial results

[0132]

[0133] As shown in Table 1, the functional hydrogel dressings prepared in Examples 4 to 6 of the present invention have better skin color regulating effects than the negative control and the positive control.

[0134] The above-described embodiments are merely descriptions of preferred implementations of the present invention, and are not intended to limit the scope of the present invention. Without departing from the spirit of the present invention, various modifications and alterations made by those skilled in the art to the technical solution of the present invention should fall within the scope of protection determined by the claims of the present invention. Other parts not described in detail in the present invention belong to the prior art, so they will not be described here.

[0135] Although the present invention has been described in conjunction with preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can implement various changes, substitutions and modifications to the subject matter listed here without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention shall be based on the scope defined by the claims.

Claims

1. A composition for improving skin condition, characterized in that: The composition comprises the following components in parts by weight: 2-15 parts of niacinamide, 0.1-2 parts of allantoin, 0.5-8 parts of total Centella asiatica glycosides, 2-10 parts of Coptis chinensis extract, 1-8 parts of Portulaca oleracea extract, 1-5 parts of ceramide, 0.5-7 parts of sodium hyaluronate, add water to 100 parts.

2. The composition for improving skin condition according to claim 1, characterized in that: The weight ratio of the total glucosides of Centella asiatica, the extract of Coptis chinensis and the extract of Portulaca oleracea is (5-8):(5-10):(3-7).

3. The composition for improving skin condition according to claim 2, characterized in that: The weight ratio of the total glucosides of Centella asiatica, the extract of Coptis chinensis and the extract of Portulaca oleracea is 6.4:8.9:6.

3.

4. The method for preparing the composition for improving skin condition according to any one of claims 1 to 3, characterized in that: The specific steps are as follows: Mix niacinamide, allantoin, ceramide and water evenly, and stir for 5 to 15 minutes at a temperature of 30 to 40°C; then add total glucosides of Centella asiatica, coptis extract and purslane extract, and stir for 5 to 15 minutes at a temperature of 30 to 40°C; then add sodium hyaluronate, and stir for 1 to 10 minutes at a temperature of 30 to 40°C; cool to room temperature to obtain the product.

5. A functional hydrogel dressing, characterized in that: The functional hydrogel dressing comprises the composition for improving skin condition according to any one of claims 1 to 3 and a thermosensitive hydrogel carrier.

6. The functional hydrogel dressing according to claim 5, characterized in that: The temperature-sensitive hydrogel carrier is chitosan and β-glycerophosphate disodium hydrate.

7. The method for preparing the functional hydrogel dressing according to claim 5 or 6, characterized in that: The following steps are involved: Step S1, dissolving chitosan in a citric acid aqueous solution with a mass concentration of 1%, stirring until the chitosan is completely dissolved, and preparing a chitosan solution with a mass concentration of 4-6%; Step S2, dissolving β-glycerophosphate disodium hydrate in deionized water to prepare a β-glycerophosphate disodium hydrate solution with a mass concentration of 1 to 2%; Step S3, mixing the chitosan solution obtained in step S1 with the β-glycerophosphate disodium hydrate solution obtained in step S2 to obtain a mixed solution, adding the composition for improving skin condition according to any one of claims 1 to 3 to the mixed solution, stirring evenly to obtain an aqueous phase; Step S4, mixing the liquid paraffin and the emulsifier evenly to obtain an oil phase; Step S5, mixing the water phase obtained in step S3 and the oil phase obtained in step S4 at a temperature of 35 to 40° C., dropping glycerol, and stirring for 28 to 35 minutes to carry out a cross-linking reaction to obtain the product.

8. The method for preparing the functional hydrogel dressing according to claim 7, characterized in that: In step S3, the volume ratio of the chitosan solution to the β-glycerophosphate disodium hydrate solution is 1:2, and the volume ratio of the mixed solution to the composition for improving skin condition is (1-2):

2.

9. Use of the composition for improving skin condition according to any one of claims 1 to 3 or the functional hydrogel dressing according to claim 5 or 6 in the preparation of skin care products.

10. The use according to claim 9, characterized in that The skin care product is an anti-inflammatory, antioxidant, and skin color regulating skin care product, and the composition for improving skin condition described in any one of claims 1 to 3 or the functional hydrogel dressing described in claim 5 or 6 is added to the skin care product in an amount of 5 to 50% by mass.

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