Traditional Chinese medicine composition, preparation method thereof and application of traditional Chinese medicine composition in preparation of medicine for treating depression

Abstract

The invention relates to the technical field of biological medicines, and particularly discloses a traditional Chinese medicine composition, a preparation method thereof and application of the traditional Chinese medicine composition in preparation of medicines for treating depression. The traditional Chinese medicine composition is prepared from the following traditional Chinese medicine raw materials: radix curcumae, poria cocos, polygala tenuifolia, andrographis paniculata and schisandra chinensis. Furthermore, the traditional Chinese medicine composition further comprises rhizoma corydalis and rhizoma coptidis. The traditional Chinese medicine composition disclosed by the invention has the effect of treating depression; therefore, the traditional Chinese medicine composition serving as an active ingredient has important application value in preparation of the medicine for treating depression.

Description

Technical Field

[0001] The present invention relates to the technical field of biomedicine, and in particular to a Chinese medicine composition and a preparation method thereof and an application thereof in preparing a medicine for treating depression. Background Art

[0002] Depression, also known as depressive disorder, is characterized by significant and persistent low mood. Long-term depression can also cause a series of complications, such as the occurrence of hyperglycemia. At present, the treatment of depression mainly includes physical therapy, psychotherapy and drug therapy; among these treatments, drug therapy is the main treatment method, which generally takes effect within 2 to 4 weeks. In the drug treatment of depression, Western medicines such as fluoxetine, sertraline, paroxetine, fluvoxamine, citalopram, and escitalopram are mainly used; and long-term use has more or less side effects and dependence; in addition, these drugs do not have the effect of lowering blood sugar.

[0003] Compared with Western medicine, Chinese medicine has attracted much attention due to its fewer side effects and wider range of treatments;

[0004] Curcuma, Poria, Polygala and Schisandra are commonly used Chinese medicines for treating depression. For example, Chinese invention patent CN201610159883.X discloses a Chinese medicine composition for treating postpartum depression, which is made of Acorus calamus, Ophiopogon japonicus, Chuanxiong, Poria, Ziziphus jujuba seeds, Schisandra chinensis, Salvia miltiorrhiza, Curcuma, Tangerine peel, Citrus aurantium, Polygala, Licorice, Mint and Chrysanthemum. The medicine composition has a good effect in treating postpartum depression, and has fewer adverse reactions than commonly used antidepressants in Western medicine. However, there is no report in the prior art that the composition has a hypoglycemic effect. Summary of the invention

[0005] In order to overcome at least one of the technical problems existing in the prior art, the present invention provides a traditional Chinese medicine composition and a preparation method thereof.

[0006] The technical solution of the present invention is as follows:

[0007] The present invention firstly provides a traditional Chinese medicine composition, which is prepared from the following traditional Chinese medicine raw materials: turmeric, tuckahoe, polygala, andrographis paniculata and schisandra chinensis.

[0008] The inventors have selected a Chinese medicine composition prepared from five Chinese medicinal materials, namely, Curcuma, Poria, Polygala, Andrographis paniculata and Schisandra chinensis, from a large number of Chinese medicinal raw materials with the effect of treating depression; further studies have shown that the Chinese medicine composition also has the effect of lowering blood sugar.

[0009] Preferably, the Chinese medicine composition is made of the following Chinese medicinal materials in parts by weight:

[0010] 10-20 parts of Curcuma Radix; 10-20 parts of Poria cocos; 8-15 parts of Polygala tenuifolia; 8-15 parts of Andrographis paniculata; 5-10 parts of Schisandra chinensis.

[0011] More preferably, the Chinese medicine composition is made of the following Chinese medicinal materials in parts by weight:

[0012] 12-18 parts of Curcuma Radix; 12-18 parts of Poria; 10-12 parts of Polygala Tenuifolia; 10-12 parts of Andrographis paniculata

[0013] 8-10 portions of Schisandra chinensis.

[0014] Most preferably, the Chinese medicine composition is made from the following Chinese medicinal materials in parts by weight:

[0015] 15 parts of Curcuma Radix; 15 parts of Poria; 10 parts of Polygala; 10 parts of Andrographis Herba; 8 parts of Schisandrae Chinensis.

[0016] Preferably, the traditional Chinese medicine composition also contains Corydalis yanhusuo and Coptis chinensis.

[0017] The inventors' further research shows that the Chinese medicine composition prepared with seven Chinese medicinal materials, namely, Curcuma, Poria, Polygala, Andrographis, Schisandra, Corydalis and Coptis, as raw materials, by further adding Corydalis and Coptis to the five Chinese medicinal materials, namely, Curcuma, Poria, Polygala, Andrographis, Schisandra, Corydalis and Coptis, has a significantly higher blood sugar-lowering effect than the Chinese medicine composition prepared with the five Chinese medicinal materials, namely, Curcuma, Poria, Polygala, Andrographis and Schisandra, as raw materials.

[0018] More preferably, the Chinese medicine composition is made of the following Chinese medicinal materials in parts by weight:

[0019] 10-20 parts of Curcuma Radix; 10-20 parts of Poria; 8-15 parts of Polygala Tenuifolia; 8-15 parts of Andrographis paniculata; 5-10 parts of Schisandrae Chinensis; 10-20 parts of Corydalis yanhusuo; 8-15 parts of Coptis chinensis.

[0020] More preferably, the Chinese medicine composition is made of the following Chinese medicinal materials in parts by weight:

[0021] 12-18 parts of Curcuma Radix; 12-18 parts of Poria; 10-12 parts of Polygala Tenuifolia; 10-12 parts of Andrographis paniculata

[0022] 8-10 parts of Schisandrae Chinensis; 12-18 parts of Corydalis; 10-12 parts of Coptis Chinensis.

[0023] Most preferably, the Chinese medicine composition is made from the following Chinese medicinal materials in parts by weight:

[0024] 15 parts of Curcuma Radix; 15 parts of Poria; 10 parts of Polygala; 10 parts of Andrographis Herba; 8 parts of Schisandrae Chinensis; 15 parts of Corydalis; 10 parts of Coptidis Rhizoma.

[0025] The present invention also provides a method for preparing the above-mentioned Chinese medicine composition, which comprises the following steps:

[0026] After mixing the Chinese medicinal materials, they are extracted with a solvent, and after the extraction is completed, the extract is filtered and separated.

[0027] The extract is concentrated and dried to obtain the traditional Chinese medicine composition.

[0028] Preferably, the total weight of the Chinese medicine raw materials and the amount of the solvent used are in a ratio of 1kg:5 to 15L.

[0029] Most preferably, the ratio of the total weight of the Chinese medicine raw materials to the amount of the solvent is 1kg:10L.

[0030] Preferably, the solvent is water or ethanol.

[0031] Preferably, the solvent consists of methanol, cyclohexane and ethyl acetate.

[0032] More preferably, the volume ratio of methanol, cyclohexane and ethyl acetate is 4-6:4-6:2-4.

[0033] Most preferably, the volume ratio of methanol, cyclohexane and ethyl acetate is 5:5:3.

[0034] The inventors found in further research that in the preparation process of the Chinese medicine composition of the present invention, the selection of the extraction solvent plays an important role in the hypoglycemic effect of the prepared Chinese medicine composition; the study showed that the hypoglycemic effect of the Chinese medicine composition obtained by extraction with a ternary mixed solvent such as methanol, cyclohexane and ethyl acetate is much higher than that of the Chinese medicine composition obtained by conventional water or ethanol extraction.

[0035] Preferably, the Chinese medicine composition is a composition having the effect of treating depression.

[0036] Preferably, the composition is a composition having an inhibitory effect on α-glucosidase.

[0037] Preferably, the composition is a composition having a blood sugar lowering effect.

[0038] The present invention also provides an application of the above-mentioned traditional Chinese medicine composition in preparing a medicine for treating depression.

[0041] The present invention also provides an application of the traditional Chinese medicine composition in preparing a medicine having an inhibitory effect on α-glucosidase.

[0042] The present invention also provides an application of the Chinese medicine composition in preparing a medicine having a blood sugar lowering effect.

[0043] Beneficial effects: The present invention provides a new Chinese medicine composition; the Chinese medicine composition prepared from five Chinese medicinal materials, namely, Curcuma, Poria, Polygala, Andrographis, and Schisandra, has the effect of treating depression; and also has the effect of lowering blood sugar. In particular, the Chinese medicine composition prepared from seven Chinese medicinal materials, namely, Curcuma, Poria, Polygala, Andrographis, Schisandra, Corydalis, and Coptis, by further adding Corydalis and Coptis, has a further significant improvement in its hypoglycemic effect; therefore, the Chinese medicine composition of the present invention as an active ingredient for preparing a drug for treating depression or for preparing a drug for lowering blood sugar has important application value. DETAILED DESCRIPTION

[0044] The present invention is further explained below in conjunction with specific examples, but the examples do not limit the present invention in any form.

[0045] Example 1 Chinese medicine composition

[0046] The Chinese medicinal raw materials are composed of 15 parts of Curcuma aromatica, 15 parts of Poria cocos, 10 parts of Polygala tenuifolia, 10 parts of Andrographis paniculata, and 8 parts of Schisandra chinensis.

[0047] Preparation method: Take the above-mentioned Chinese medicines and mix them evenly, then heat and reflux them with water at 100°C for 1 hour, filter and separate the extract after the extraction is completed, and concentrate and dry the extract to obtain the Chinese medicine composition; the total weight of the Chinese medicine raw materials and the amount of water used is 1kg:10L.

[0048] Example 2 Chinese medicine composition

[0049] The Chinese medicinal raw materials are composed of 15 parts of Curcuma, 15 parts of Poria, 10 parts of Polygala, 10 parts of Andrographis, 8 parts of Schisandra, 15 parts of Corydalis and 10 parts of Coptis.

[0050] Preparation method: Take the above-mentioned Chinese medicines and mix them evenly, then heat and reflux them with water at 100°C for 1 hour, filter and separate the extract after the extraction is completed, and concentrate and dry the extract to obtain the Chinese medicine composition; the total weight of the Chinese medicine raw materials and the amount of water used is 1kg:10L.

[0051] Example 3 Chinese medicine composition

[0052] The Chinese medicinal raw materials are composed of 15 parts of Curcuma, 15 parts of Poria, 10 parts of Polygala, 10 parts of Andrographis, 8 parts of Schisandra, 15 parts of Corydalis and 10 parts of Coptis.

[0053] Preparation method: take the above-mentioned Chinese medicine and mix them evenly, then heat and reflux them with a 95% by volume ethanol aqueous solution at 75°C for 1 hour, filter and separate the extract after the extraction is completed, and concentrate and dry the extract to obtain the Chinese medicine composition; the total weight of the Chinese medicine raw materials and the volume fraction of 95% by volume ethanol aqueous solution The ratio is 1kg:10L.

[0054] Example 4 Chinese medicine composition

[0055] The Chinese medicinal raw materials are composed of 15 parts of Curcuma, 15 parts of Poria, 10 parts of Polygala, 10 parts of Andrographis, 8 parts of Schisandra, 15 parts of Corydalis and 10 parts of Coptis.

[0056] Preparation method: take the above Chinese medicines and mix them evenly, then heat and reflux them with a solvent at 75°C for 1 hour, filter and separate the extract after extraction, and concentrate and dry the extract to obtain the Chinese medicine composition;

[0057] The total weight of the Chinese medicinal raw materials and the amount of the solvent used are 1kg:10L;

[0058] The solvent consists of methanol, cyclohexane and ethyl acetate in a volume ratio of 5:5:3.

[0059] Comparative Example 1 Chinese medicine composition

[0060] The Chinese medicinal raw materials are composed of 15 parts of Curcuma, 15 parts of Poria, 10 parts of Polygala, 10 parts of Andrographis paniculata, 8 parts of Schisandra chinensis and 15 parts of Corydalis yanhusuo.

[0061] Preparation method: Take the above-mentioned Chinese medicines and mix them evenly, then heat and reflux them with water at 100°C for 1 hour, filter and separate the extract after the extraction is completed, and concentrate and dry the extract to obtain the Chinese medicine composition; the total weight of the Chinese medicine raw materials and the amount of water used is 1kg:10L.

[0062] Comparative Example 2 Chinese medicine composition

[0063] The Chinese medicinal raw materials are composed of 15 parts of Curcuma, 15 parts of Poria, 10 parts of Polygala, 10 parts of Andrographis paniculata, 8 parts of Schisandra chinensis and 10 parts of Coptis chinensis.

[0064] Preparation method: Take the above-mentioned Chinese medicines and mix them evenly, then heat and reflux them with water at 100°C for 1 hour, filter and separate the extract after the extraction is completed, and concentrate and dry the extract to obtain the Chinese medicine composition; the total weight of the Chinese medicine raw materials and the amount of water used is 1kg:10L.

[0065] Comparative Example 3 Chinese medicine composition

[0066] The Chinese medicinal raw materials are composed of 15 parts of Curcuma, 15 parts of Poria, 10 parts of Polygala, 10 parts of Andrographis, 8 parts of Schisandra, 15 parts of Corydalis and 10 parts of Coptis.

[0067] Preparation method: take the above Chinese medicines and mix them evenly, then heat and reflux them with a solvent at 75°C for 1 hour, filter and separate the extract after extraction, and concentrate and dry the extract to obtain the Chinese medicine composition;

[0068] The total weight of the Chinese medicinal raw materials and the amount of the solvent used are 1kg:10L;

[0069] The solvent consists of methanol and cyclohexane in a volume ratio of 5:5.

[0070] Comparative Example 4 Chinese medicine composition

[0071] The Chinese medicinal raw materials are composed of 15 parts of Curcuma, 15 parts of Poria, 10 parts of Polygala, 10 parts of Andrographis, 8 parts of Schisandra, 15 parts of Corydalis and 10 parts of Coptis.

[0072] Preparation method: take the above Chinese medicines and mix them evenly, then heat and reflux them with a solvent at 75°C for 1 hour, filter and separate the extract after extraction, and concentrate and dry the extract to obtain the Chinese medicine composition;

[0073] The total weight of the Chinese medicinal raw materials and the amount of the solvent used are 1kg:10L;

[0074] The solvent consists of methanol and ethyl acetate in a volume ratio of 5:3.

[0075] Comparative Example 5 Chinese medicine composition

[0076] The Chinese medicinal raw materials are composed of 15 parts of Curcuma, 15 parts of Poria, 10 parts of Polygala, 10 parts of Andrographis, 8 parts of Schisandra, 15 parts of Corydalis and 10 parts of Coptis.

[0077] Preparation method: take the above Chinese medicines and mix them evenly, then heat and reflux them with a solvent at 75°C for 1 hour, filter and separate the extract after extraction, and concentrate and dry the extract to obtain the Chinese medicine composition;

[0078] The total weight of the Chinese medicinal raw materials and the amount of the solvent used are 1kg:10L;

[0079] The solvent consists of cyclohexane and ethyl acetate in a volume ratio of 5:3.

[0080] Experimental Example 1 α-glucosidase inhibition activity experiment

[0081] Determination of α-glucosidase inhibitory activity

[0082] 500 μL of phosphate buffer solution (pH 6.8, 0.067 mol / L), 500 μL of α-glucosidase (0.1 U / mL) and 100 μL of the sample solution to be tested (50 μg / mL) were mixed evenly, and then 500 μL of pNPG (4.0 mmol / L) was added after incubation at 37°C for 20 min. After mixing evenly, the mixture was reacted at 37°C for 30 min. After the reaction was completed, methanol was added to terminate the reaction. The concentration of pNPG was detected, and the inhibition rate of α-glucosidase was calculated.

[0083] α-glucosidase inhibition rate (%) = [(C a -C b +C c ) / C a ]×100%; among which, C a is the concentration of pNPG after the reaction of buffer solution, enzyme solution and substrate; C b is the concentration of pNPG after the sample reacts with the enzyme solution and substrate; C c is the concentration of pNPG after the sample reacts with the buffer and substrate. The test results are shown in Table 1.

[0084] Table 1. α-glucosidase inhibition rate experimental results

[0085]

[0086] It can be seen from the experimental results in Table 1 that the Chinese medicine composition prepared in Example 1 tested in Experimental Group 1 has a certain inhibitory effect on α-glucosidase; this shows that the Chinese medicine composition prepared from five Chinese medicinal materials, namely, Curcuma, Poria, Polygala, Andrographis paniculata and Schisandra chinensis, has a certain blood sugar-lowering effect.

[0087] From the experimental results in Table 1, it can be seen that the α-glucosidase inhibition rates of experimental groups 2 and 3 are further significantly higher than those of experimental group 1; this indicates that the Chinese medicine composition prepared with 7 Chinese medicinal materials, namely, Curcuma, Poria, Polygala, Andrographis, Schisandra, Corydalis and Coptis as raw materials, after further adding Corydalis and Coptis to the 5 Chinese medicinal materials, namely, Curcuma, Poria, Polygala, Andrographis, Schisandra, Corydalis and Coptis, has a significantly higher hypoglycemic effect than the Chinese medicine composition prepared with 5 Chinese medicinal materials, namely, Curcuma, Poria, Polygala, Andrographis and Schisandra as raw materials.

[0088] From the experimental results in Table 1, it can be seen that the α-glucosidase inhibition rates of experimental groups 5 and 6 are improved compared with those of experimental group 1, but the improvement is not significant; the increase is significantly lower than that of experimental group 2. This shows that: when Corydalis and Coptis chinensis must be added to the five Chinese medicinal materials of Curcuma, Poria, Polygala, Andrographis and Schisandra, the hypoglycemic effect can be significantly higher than that of the Chinese medicine composition prepared from the five Chinese medicinal materials of Curcuma, Poria, Polygala, Andrographis and Schisandra as raw materials; however, when only Corydalis or Coptis chinensis is added to the five Chinese medicinal materials of Curcuma, Poria, Polygala, Andrographis and Schisandra, the hypoglycemic effect is not significantly higher than that of the Chinese medicine composition prepared from the five Chinese medicinal materials of Curcuma, Poria, Polygala, Andrographis and Schisandra as raw materials.

[0089] It can be seen from the experimental results in Table 1 that the α-glucosidase inhibition rate of experimental group 4 is further significantly higher than that of experimental groups 2 and 3; this shows that: in the preparation process of the Chinese medicine composition of the present invention, the selection of the extraction solvent plays an important role in the hypoglycemic effect of the prepared Chinese medicine composition; the above experimental results show that the Chinese medicine composition obtained by extraction with a ternary mixed solvent such as methanol, cyclohexane and ethyl acetate has a significantly higher hypoglycemic effect than the Chinese medicine composition obtained by conventional water or ethanol extraction.

[0090] It can be seen from the experimental results in Table 1 that the α-glucosidase inhibition rate of experimental groups 7 to 9 is improved compared with experimental groups 2 and 3, but the improvement is not large; the improvement is much smaller than that of experimental group 4. This shows that: in the preparation process of the Chinese medicine composition of the present invention, the selection of the extraction solvent plays an important role in the hypoglycemic effect of the prepared Chinese medicine composition; the above experimental results show that the Chinese medicine composition extracted by a ternary mixed solvent such as methanol, cyclohexane and ethyl acetate must be used to obtain a Chinese medicine composition, and its hypoglycemic effect can be greatly higher than that of the Chinese medicine composition extracted by conventional water or ethanol; however, the Chinese medicine composition extracted by a combination of any two of the three solvents such as methanol, cyclohexane and ethyl acetate cannot have a significantly higher hypoglycemic effect than that of the Chinese medicine composition extracted by conventional water or ethanol.

Claims

1. A Chinese medicine composition, characterized in that: It is made from the following Chinese medicinal materials: Curcuma, Poria, Polygala, Andrographis paniculata and Schisandra chinensis.

2. The Chinese medicine composition according to claim 1, characterized in that: Made from the following Chinese medicinal materials in parts by weight: 10-20 parts of Curcuma Radix; 10-20 parts of Poria cocos; 8-15 parts of Polygala tenuifolia; 8-15 parts of Andrographis paniculata; 5-10 parts of Schisandra chinensis.

3. The Chinese medicine composition according to claim 2, characterized in that: Made from the following Chinese medicinal materials in parts by weight: 12-18 parts of Curcuma Radix; 12-18 parts of Poria; 10-12 parts of Polygala Tenuifolia; 10-12 parts of Andrographis paniculata 8-10 portions of Schisandra chinensis. Most preferably, the Chinese medicine composition is made from the following Chinese medicinal materials in parts by weight: 15 parts of Curcuma Radix; 15 parts of Poria; 10 parts of Polygala; 10 parts of Andrographis Herba; 8 parts of Schisandrae Chinensis.

4. The Chinese medicine composition according to claim 1, characterized in that: The traditional Chinese medicine composition also contains Corydalis yanhusuo and Coptis chinensis.

5. The Chinese medicine composition according to claim 4, characterized in that: Made from the following Chinese medicinal materials in parts by weight: 10-20 parts of Curcuma Radix; 10-20 parts of Poria; 8-15 parts of Polygala Tenuifolia; 8-15 parts of Andrographis paniculata; 5-10 parts of Schisandrae Chinensis; 10-20 parts of Corydalis yanhusuo; 8-15 parts of Coptis chinensis.

6. The Chinese medicine composition according to claim 4, characterized in that: Made from the following Chinese medicinal materials in parts by weight: 12-18 parts of Curcuma Radix; 12-18 parts of Poria; 10-12 parts of Polygala Tenuifolia; 10-12 parts of Andrographis paniculata 8-10 parts of Schisandrae Chinensis; 12-18 parts of Corydalis; 10-12 parts of Coptis Chinensis. Most preferably, the Chinese medicine composition is made from the following Chinese medicinal materials in parts by weight: 15 parts of Curcuma Radix; 15 parts of Poria; 10 parts of Polygala; 10 parts of Andrographis Herba; 8 parts of Schisandrae Chinensis; 15 parts of Corydalis; 10 parts of Coptidis Rhizoma.

7. The method for preparing the Chinese medicine composition according to any one of claims 1 to 6, characterized in that: The following steps are included: After mixing the Chinese medicinal materials, they are extracted with a solvent, and after the extraction is completed, the extract is filtered and separated. The extract is concentrated and dried to obtain the traditional Chinese medicine composition.

8. The preparation method according to claim 7, characterized in that: The total weight of the Chinese medicine raw materials and the amount of the solvent used are in a ratio of 1kg:5-15L. Most preferably, the ratio of the total weight of the Chinese medicine raw materials to the amount of the solvent is 1kg:10L.

9. The preparation method according to claim 7, characterized in that: The solvent is water or ethanol.

10. Use of the Chinese medicine composition according to any one of claims 1 to 9 in the preparation of a drug for treating depression.

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