Efficient bio-organic fertilizer and preparation method thereof
Through freezing pretreatment, microwave treatment and puffing technology, animal corpses are converted into efficient biological organic fertilizer, solving the problems of long treatment cycles, low efficiency and environmental pollution in the existing technology, achieving efficient and environmentally friendly organic fertilizer production, improving Soil fertility and crop yield.
Patent Information
- Application Number
- CN202411666170.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-20
- Publication Date
- 2025-02-18
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
The prior art has problems such as long treatment cycle, low efficiency, low fertilizer efficiency and possible harmful substances when converting animal carcasses into organic fertilizers, resulting in environmental pollution and economic losses.
Refrigeration pretreatment, microwave treatment and puffing technology are used to convert animal carcasses into bio-organic fertilizers rich in nutrients. Specific steps include hair removal, bone removal, freezing, crushing, bacterial infusion, microwave treatment, puffing, fermentation and formulation, using composite fermentation agents and microbial flora to improve fermentation efficiency and the quality of organic fertilizers.
It significantly shortens the processing cycle of converting animal carcasses into organic fertilizers, improves treatment efficiency and the quality of organic fertilizers, avoids the production of harmful substances, improves soil structure and fertility, and promotes the growth and development of crops.
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Figure CN119462258A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the field of biological fertilizers, and in particular to a high-efficiency biological organic fertilizer and a preparation method thereof. Background Art
[0002] With the development of agricultural waste resource utilization technology, converting animal carcasses into organic fertilizer has become a more environmentally friendly and sustainable solution.
[0003] However, existing methods of converting animal carcasses into organic fertilizers often have some problems. For example, some methods have a long processing cycle and low efficiency; some methods produce organic fertilizers with low fertilizer efficiency and are difficult to meet the growth needs of crops; and some methods may produce harmful substances during the processing process, causing secondary pollution to the environment.
[0004] At the same time, the traditional method of using animal carcasses to prepare biological organic fertilizers is usually to directly crush the animal carcasses and pile them up for fermentation. In order to further improve the fermentation efficiency, some people have tried to pulp the animal carcasses. Although these treatment methods are simple, in addition to the low fermentation effect and long fermentation time, there is also a thorny problem. That is, during the entire treatment process, the stench of the animal carcasses is everywhere, polluting the environment and causing discomfort to the staff. On the other hand, in the process of pulping the animal carcasses, many organic substances in the animal carcasses are easily lost, causing environmental pollution while also causing economic losses. Summary of the invention
[0005] In order to overcome the shortcomings of the prior art, the present invention proposes a high-efficiency biological organic fertilizer and a preparation method thereof. The method uses animal carcasses as the main raw material, and converts them into nutrient-rich and harmless biological organic fertilizers through freezing pretreatment, microwave treatment, and puffing technology. The organic fertilizer not only has a high fertilizer effect, but also can improve soil structure, increase soil fertility, and promote the growth and development of crops. The details are as follows:
[0006] A high-efficiency bio-organic fertilizer, which is prepared by processing animal carcasses, and the preparation method comprises the following steps:
[0007] S1. Pretreatment: remove hair and bones from the animal carcass and freeze it at -20℃ to -25℃ for 5-7 days to completely freeze it;
[0008] S2, crushing: cutting and crushing the frozen animal carcass to obtain frozen fragments;
[0009] S3, inoculation: evenly mixing the frozen pieces with the composite starter to obtain mixed frozen pieces;
[0010] The composite fermentation agent is composed of an enzyme composite preparation, a microbial composite bacterial agent, and nano-cerium oxide; according to the mass ratio, the enzyme composite preparation: the microbial composite bacterial agent: the nano-cerium oxide = 8-12: 80-90: 5-8;
[0011] S4, microwave treatment: subjecting the mixed frozen pieces to microwave treatment for 10 min-15 min; the microwave power is 10 kW-30 kW, and the microwave frequency is 2400 MHz-2500 MHz;
[0012] S5, puffing: after the microwave treatment, immediately pressurize to 5-8MPa and maintain for 20min-25min, then suddenly reduce the pressure to make it puffed;
[0013] S6, fermentation: in a sealed container, at 30°C-50°C, ferment for 5d-6d to obtain fermented organic fertilizer; humidity is maintained at 60%-70%, pH is 6.5-8.5;
[0014] S7, preparation: after mixing the fermented organic fertilizer with humic acid, decomposed crop straw and rice bran, 2%-3% of microbial flora is added to obtain a high-efficiency biological organic fertilizer; the microbial flora is a rhizobium agent, a nitrogen-fixing bacteria agent, a phosphorus bacteria agent and a silicate bacteria agent. In addition, in the step S2, the frozen animal carcass is cut and broken into pieces of 2-4cm×2-4cm to obtain frozen pieces.
[0015] Moreover, in the step S3, the enzyme complex preparation is composed of collagenase, keratinase and lipase, and the mass ratio is collagenase: keratinase: lipase = 5-10: 20-30: 1-5.
[0016] Moreover, the enzyme compound preparation is composed of collagenase, keratinase and lipase, and the mass ratio is collagenase: keratinase: lipase = 8:25:3;
[0017] Moreover, in step S3, the microbial composite agent is bacillus, lactic acid bacteria, yeast, filamentous fungi, and actinomycetes, and the mass ratio is bacillus: lactic acid bacteria: yeast: filamentous fungi: actinomycetes = 4-6: 3-5: 2-4: 1-3: 3-6.
[0018] Moreover, in step S3, the microbial composite agent is bacillus, lactic acid bacteria, yeast, filamentous fungi, and actinomycetes, and the mass ratio of bacillus: lactic acid bacteria: yeast: filamentous fungi: actinomycetes is 5:4:2-4:2:5.
[0019] Moreover, in the step S7, the mass ratio of the fermented organic fertilizer to the humic acid, the decomposed crop straw and the rice bran is 6-10:2-4:3-6:1-3.
[0020] Moreover, in step S7, the mass ratio of the rhizobium agent, the nitrogen-fixing bacteria agent, the phosphorus bacteria agent and the silicate bacteria agent is 1-3:1-2:2-4:1-3.
[0021] Moreover, in step S7, the mass ratio is 2:1.5:3:2.
[0022] Moreover, in the step S7, after the fermented organic fertilizer is mixed with humic acid, decomposed crop straw and rice bran, 2%-3% of microbial flora is added, and then chicken manure fermentation is added to obtain a high-efficiency biological organic fertilizer; the chicken manure fermentation takes chicken manure as the main fermentation raw material, adds a fermentation agent (Gimbobe organic fertilizer fermentation agent), a regulator (corn flour, rice bran, wheat bran, etc.), and corn straw (for adjusting the moisture content and looseness of the fermented material), and then water is added to make the moisture content about 60%, and the product is dried after being piled up and fermented for 7 days.
[0023] Moreover, the pH value of the high-efficiency biological organic fertilizer is 7.5-8.5 and the humidity is 20%-30%.
[0024] Compared with the prior art, the present invention has the following beneficial effects:
[0025] 1. The present invention uses freezing pretreatment, microwave treatment and puffing technology. During the microwave treatment and puffing process, the composite fermentation agent fully and evenly penetrates into the interior of the animal carcass fragments and fully reacts with the animal carcass fragments, further accelerating the fermentation speed and effectively shortening the processing cycle of converting animal carcasses into organic fertilizers. The traditional animal carcass fermentation time is about half a month, while the present invention only takes 5-6 days to complete, which significantly improves the processing efficiency, greatly reduces the fermentation time, and makes the production of organic fertilizers faster and more efficient.
[0026] 2. The present invention adopts a composite fermentation agent for fermentation, which is composed of an enzyme composite preparation, a microbial composite agent and nano cerium oxide, wherein the enzyme composite preparation is composed of collagenase, keratinase and lipase; the microbial composite agent is composed of Bacillus, lactic acid bacteria, yeast, filamentous fungi and actinomycetes; the enzyme composite preparation can preliminarily decompose the macromolecular organic matter in the animal carcasses, provide a more easily usable substrate for the microbial composite agent, thereby accelerating the fermentation process and improving the quality and yield of the organic fertilizer; nano cerium oxide can promote the growth and reproduction of microorganisms and improve the activity of microorganisms. At the same time, during the fermentation process, nano cerium oxide also acts as a catalyst to accelerate the decomposition and conversion of organic matter during the fermentation process; during the fermentation process, nano cerium oxide can also improve the material structure, making it easier to be decomposed and utilized by microorganisms; moreover, nano cerium oxide has excellent antioxidant properties, can protect microorganisms from damage by oxidative stress, and help maintain the stability and safety of organic fertilizers; the enzyme composite preparation, the microbial composite agent and nano cerium oxide work together to effectively decompose the organic matter in the animal carcasses and convert them into nutrients that are easily absorbed by plants.
[0027] 3. The pretreatment process of the present invention is carried out under the condition of freezing the animal carcasses, and the microwave treatment and puffing technology adopted by the present invention not only helps to remove the rancid smell of the animal carcasses, but also reduces the loss of organic matter, reduces environmental pollution and economic losses. In addition, the prepared high-efficiency biological organic fertilizer itself will not cause pollution to the soil and groundwater. The method of the present invention effectively avoids the generation of harmful substances during the treatment process and reduces the risk of environmental pollution.
[0028] 4. The high-efficiency biological organic fertilizer of the present invention is rich in organic matter and microbial flora (rhizobia, nitrogen-fixing bacteria, phosphate bacteria and silicate bacteria), which can improve soil structure, increase soil fertility and air permeability, promote root growth and nutrient absorption of crops, and can also enhance the water retention capacity and salt-alkali resistance of the soil after long-term use, providing a good environment for the healthy growth of crops.
[0029] 5. Since the high-efficiency biological organic fertilizer of the present invention has the characteristics of high fertilizer efficiency and balanced nutrition, it can significantly improve the yield and quality of crops. At the same time, the microbial flora therein can also inhibit pathogens and pests in the soil, reduce the use of pesticides, and improve the safety and market competitiveness of agricultural products.
[0030] 6. The organic fertilizer provided by the present invention contains chicken manure fermentation product, which is rich in organic matter, nitrogen, phosphorus, potassium and other essential nutrients for plants, as well as a variety of trace elements and beneficial biologically active bacteria. These nutrients are released and transformed during the fermentation process and are easier to be absorbed and utilized by plants. Compared with chemical fertilizers, the fertilizer effect of organic fertilizers is more lasting, and can continuously provide nutrients for plants, promoting the healthy growth and high yield of crops; after the organic matter in the chicken manure fermentation product is decomposed in the soil, humus can be formed, which is conducive to the formation of soil aggregate structure, increases soil porosity, and improves soil water retention, air permeability and fertilizer retention; chicken manure fermentation product contains a large number of beneficial microorganisms, which reproduce in large numbers in the soil, can secrete antibiotic substances, inhibit the growth of pathogens and pests in the soil, and reduce the occurrence of soil-borne diseases. At the same time, they can also activate nitrogen in the air, decompose and release insoluble phosphorus and potassium nutrients, and provide comprehensive nutritional support for crops. BRIEF DESCRIPTION OF THE DRAWINGS
[0031] Figure 1 The present invention is a flow chart for preparing the high-efficiency biological organic fertilizer. DETAILED DESCRIPTION
[0032] Example 1
[0033] A high-efficiency bio-organic fertilizer, which is prepared by processing animal carcasses, and the preparation method comprises the following steps:
[0034] S1. Pretreatment: remove hair and bones from the animal carcass and freeze it at -20°C for 7 days to completely freeze it;
[0035] S2, crushing: cutting and crushing the frozen animal carcass to obtain frozen fragments;
[0036] S3, inoculation: evenly mixing the frozen pieces with the composite starter to obtain mixed frozen pieces;
[0037] The composite fermentation agent is composed of an enzyme composite preparation, a microbial composite bacterial agent, and nano-cerium oxide; according to the mass ratio, the enzyme composite preparation: the microbial composite bacterial agent: the nano-cerium oxide is 8:80:5;
[0038] S4, microwave treatment: subjecting the mixed frozen pieces to microwave treatment for 10 min; the microwave power is 10 kW, and the microwave frequency is 2400 MHz;
[0039] S5, puffing: after the microwave treatment, the pressure is immediately increased to 5 MPa, maintained for 25 min, and then the pressure is suddenly reduced to puff;
[0040] S6, fermentation: fermentation for 6 days at 30°C in a sealed container to obtain fermented organic fertilizer; humidity is maintained at 60% and pH is 6.5;
[0041] S7. Preparation: After mixing the fermented organic fertilizer with humic acid, decomposed crop straw and rice bran, 2%-3% of microbial flora is added, and then chicken manure fermentation is added to obtain a high-efficiency biological organic fertilizer; the microbial flora includes rhizobia, nitrogen-fixing bacteria, phosphate bacteria and silicate bacteria.
[0042] Preferably, in step S2, the frozen animal carcass is cut and broken into pieces of 2 cm×4 cm to obtain frozen pieces.
[0043] Preferably, in step S3, the enzyme complex preparation is composed of collagenase, keratinase and lipase, and the mass ratio of collagenase: keratinase: lipase is 5:20:1.
[0044] Preferably, in step S3, the microbial composite agent is Bacillus, lactic acid bacteria, yeast, filamentous fungi, and actinomycetes, and the mass ratio of Bacillus: lactic acid bacteria: yeast: filamentous fungi: actinomycetes is 4:3:2:1:3.
[0045] Preferably, in step S7, the mass ratio of fermented organic fertilizer to humic acid, decomposed crop straw and rice bran is 6:2:3:1.
[0046] Preferably, in step S7, the mass ratio of the rhizobium agent, the nitrogen-fixing bacteria agent, the phosphorus bacteria agent and the silicate bacteria agent is 1:1:2:1.
[0047] Preferably, the pH value of the high-efficiency biological organic fertilizer is 7.5 and the humidity is 20%.
[0048] Example 2
[0049] A high-efficiency bio-organic fertilizer, which is prepared by processing animal carcasses, and the preparation method comprises the following steps:
[0050] S1. Pretreatment: remove hair and bones from the animal carcass and freeze it at -25°C for 5 days to completely freeze it;
[0051] S2, crushing: cutting and crushing the frozen animal carcass to obtain frozen fragments;
[0052] S3, inoculation: evenly mixing the frozen pieces with the composite starter to obtain mixed frozen pieces;
[0053] The composite fermentation agent is composed of an enzyme composite preparation, a microbial composite bacterial agent, and nano-cerium oxide; according to the mass ratio, the enzyme composite preparation: the microbial composite bacterial agent: the nano-cerium oxide is 12:90:8;
[0054] S4, microwave treatment: subjecting the mixed frozen pieces to microwave treatment for 15 min; the microwave power is 10 kW, and the microwave frequency is 2500 MHz;
[0055] S5, puffing: after the microwave treatment, the pressure is immediately increased to 8 MPa, maintained for 20 minutes, and then the pressure is suddenly reduced to puff;
[0056] S6, fermentation: fermentation for 6 days at 30°C in a sealed container to obtain fermented organic fertilizer; humidity is maintained at 60% and pH is 6.5;
[0057] S7. Preparation: After mixing the fermented organic fertilizer with humic acid, decomposed crop straw and rice bran, 2% of microbial flora is added to obtain a high-efficiency biological organic fertilizer; the microbial flora includes rhizobium agent, nitrogen-fixing bacteria agent, phosphorus bacteria and silicate bacteria agent.
[0058] Preferably, in step S2, the frozen animal carcass is cut and broken into pieces of 4 cm×2 cm to obtain frozen pieces.
[0059] Preferably, in step S3, the enzyme complex preparation is composed of collagenase, keratinase and lipase, and the mass ratio of collagenase: keratinase: lipase is 10:30:5.
[0060] Preferably, in step S3, the microbial composite agent is Bacillus, lactic acid bacteria, yeast, filamentous fungi, and actinomycetes, and the mass ratio of Bacillus: lactic acid bacteria: yeast: filamentous fungi: actinomycetes is 6:5:4:3:6.
[0061] Preferably, in step S7, the mass ratio of fermented organic fertilizer to humic acid, decomposed crop straw and rice bran is 10:4:6:3.
[0062] Preferably, in step S7, the mass ratio of the rhizobium agent, the nitrogen-fixing bacteria agent, the phosphorus bacteria agent and the silicate bacteria agent is 3:2:4:3.
[0063] Preferably, the pH value of the high-efficiency biological organic fertilizer is 8.5 and the humidity is 30%.
[0064] Example 3
[0065] A high-efficiency bio-organic fertilizer, which is prepared by processing animal carcasses, and the preparation method comprises the following steps:
[0066] S1. Pretreatment: remove hair and bones from the animal carcasses and freeze them at -23°C for 6 days to completely freeze them;
[0067] S2, crushing: cutting and crushing the frozen animal carcass to obtain frozen fragments;
[0068] S3, inoculation: evenly mixing the frozen pieces with the composite starter to obtain mixed frozen pieces;
[0069] The composite fermentation agent is composed of an enzyme composite preparation, a microbial composite bacterial agent, and nano-cerium oxide; according to the mass ratio, the enzyme composite preparation: the microbial composite bacterial agent: the nano-cerium oxide is 10:85:6;
[0070] S4, microwave treatment: subjecting the mixed frozen pieces to microwave treatment for 13 min; the microwave power is 20 kW, and the microwave frequency is 2450 MHz;
[0071] S5, puffing: after the microwave treatment, the pressure is immediately increased to 7 MPa, maintained for 23 minutes, and then the pressure is suddenly reduced to puff;
[0072] S6, fermentation: fermentation for 5.5 days at 40°C in a sealed container to obtain fermented organic fertilizer; humidity is maintained at 65% and pH is 7;
[0073] S7, preparation: after mixing the fermented organic fertilizer with humic acid, decomposed crop straw and rice bran, 2.5% of microbial flora is added, wherein the microbial flora includes rhizobium agent, nitrogen-fixing bacteria agent, phosphorus bacteria and silicate bacteria agent to obtain a high-efficiency biological organic fertilizer.
[0074] Preferably, in step S2, the frozen animal carcass is cut and broken into 3 cm×3 cm pieces to obtain frozen pieces.
[0075] Preferably, in step S3, the enzyme complex preparation is composed of collagenase, keratinase and lipase, and the mass ratio of collagenase: keratinase: lipase is 8:25:3;
[0076] Preferably, in step S3, the microbial composite agent is bacillus, lactic acid bacteria, yeast, filamentous fungi, and actinomycetes, and the mass ratio of bacillus: lactic acid bacteria: yeast: filamentous fungi: actinomycetes is 5:4:2-4:2:5.
[0077] Preferably, in step S7, the mass ratio is 2:1.5:3:2.
[0078] Preferably, the pH value of the high-efficiency biological organic fertilizer is 7.5-8.5 and the humidity is 25%.
[0079] Comparative Example 1
[0080] The difference between this comparative example and Example 3 is that in step S3, the composite fermentation agent is composed of an enzyme composite preparation and a microbial composite bacterial agent; in terms of mass ratio, the enzyme composite preparation: the microbial composite bacterial agent = 10:85.
[0081] Comparative Example 2
[0082] The difference between this comparative example and Example 3 is that in step S3, the composite fermentation agent is composed of an enzyme composite preparation and nano-cerium oxide; in terms of mass ratio, the enzyme composite preparation: nano-cerium oxide = 10:6.
[0083] Comparative Example 3
[0084] The difference between this comparative example and Example 3 is that in step S3, the composite fermentation agent is composed of a microbial composite bacterial agent and nano-cerium oxide; in terms of mass ratio, the microbial composite bacterial agent: nano-cerium oxide = 85:6.
[0085] Comparative Example 4
[0086] The composite fermentation agent is composed of an enzyme composite preparation, a microbial composite bacterial agent, and nano-cerium oxide; according to the mass ratio, the enzyme composite preparation: the microbial composite bacterial agent: the nano-cerium oxide is 10:85:2;
[0087] Comparative Example 5
[0088] The composite fermentation agent is composed of an enzyme composite preparation, a microbial composite bacterial agent, and nano-cerium oxide; according to the mass ratio, the enzyme composite preparation: the microbial composite bacterial agent: the nano-cerium oxide is 10:85:15;
[0089] Comparative Example 6
[0090] The difference between this comparative example and Example 3 is that in step S3, the microwave treatment process in step S4 is not performed.
[0091] Comparative Example 7
[0092] The difference between this comparative example and Example 3 is that in step S3, the puffing process of step S5 is not performed.
[0093] Experimental Section
[0094] Experiment 1 Fermentation cycle comparison
[0095] The animal carcasses were fermented using the technical solutions of Examples 1-3 and Comparative Examples 1-7. When the temperature of the fermented material dropped to 35°C and the color and odor of the material became stable, the fermentation cycle ended. The fermentation cycles of Examples 1-3 and Comparative Examples 1-7 were recorded respectively. The results are shown in Table 1.
[0096] Table 1 Fermentation cycle results statistics
[0097]
[0098] It can be seen from Table 1 that the fermentation period of Examples 1-3 of the present invention is only 5-6 days. This is mainly due to the optimization of the ratio of the composite leavening agent, which makes the fermentation proceed more quickly. At the same time, the microwave treatment and puffing process allow the composite leavening agent to fully and evenly enter the interior of the animal carcass fragments and fully react with the animal carcass fragments, further accelerating the fermentation speed.
[0099] The composite fermentation agent of comparative example 1 is not added with nano cerium oxide, and the fermentation period is extended to 10 days. In the composite fermentation agents of comparative examples 4 and 5, the amount of nano cerium oxide added is more or less, which eventually affects the fermentation period; nano cerium oxide can promote the growth and reproduction of microorganisms and improve the activity of microorganisms. At the same time, during the fermentation process, it also acts as a catalyst to accelerate the decomposition and conversion of organic matter during the fermentation process; moreover, during the fermentation process, nano cerium oxide can also improve the material structure, making it easier to be decomposed and utilized by microorganisms; it is also found in the study that nano cerium oxide has excellent antioxidant properties and can protect microorganisms from oxidative stress; the ratio of the present invention can promote the growth and reproduction of enzyme composite preparations and microbial composite bacterial agents, and play a synergistic effect; when the amount of nano cerium oxide is too low, its synergistic effect cannot be fully exerted, and when the amount is too high, the catalytic effect of nano cerium oxide will inhibit the activity of microorganisms, thereby affecting the decomposition and conversion efficiency of organic matter during the fermentation process; on the other hand, excessive addition of nano cerium oxide may also change the thermal effect during the fermentation process, resulting in fermentation temperature fluctuations, which is not conducive to the growth and reproduction of microorganisms.
[0100] The composite fermentation agent of Comparative Example 2 only used a combination of an enzyme composite preparation and nano-cerium oxide, which was insufficient to support the fermentation process, and the experiment failed.
[0101] The composite fermentation agent of Comparative Example 3 was not added with an enzyme composite preparation, and the fermentation period was 11 days, which shows the importance of enzyme composite preparations in decomposing organic matter in animal carcasses and improving the availability of microorganisms.
[0102] Comparative Example 6 did not undergo a microwave-free treatment process, and the fermentation period was extended to 9 days. This is because microwave treatment can improve the material structure and allow the material to come into more complete contact with the microorganisms. At the same time, microwave treatment may also increase the activity of the microorganisms.
[0103] In Comparative Example 7, the puffing process was not performed, and the fermentation period was extended to 10 days. The puffing process may be helpful for further decomposition of the material and penetration of microorganisms, thereby shortening the fermentation period.
[0104] The optimized ratio of the composite fermentation agent, the introduction of microwave treatment and the puffing process are all key factors in shortening the fermentation cycle and improving the fermentation efficiency. At the same time, the experimental results also show that nano-cerium oxide, as a new type of additive, and the ratio of the present invention, have a significant positive effect in promoting the fermentation process of biological organic fertilizer.
[0105] Experiment 2 Fertilizer Efficiency Comparison
[0106] Under the same soil conditions, experimental fields were selected, and the bio-organic fertilizers of Examples 1-3 and Comparative Examples 1-7 were applied respectively, and wheat (Zhongxin Wheat 998) was planted. After one month, the plant height and the incidence of diseases and insect pests of wheat were recorded. The results are shown in Table 2.
[0107] Table 2 Statistics of fertilizer efficiency comparison results
[0108]
[0109]
[0110] It can be seen from Table 2 that the high-efficiency biological organic fertilizers in Examples 1, 2, and 3 are superior to those in Comparative Examples 1-7 in terms of plant height and incidence of pests and diseases, which indicates that the composite fermentation agent formula (comprising an enzyme composite preparation, a microbial composite agent, and nano-cerium oxide) and the preparation process (including microwave treatment and puffing steps) in the embodiments have a significant effect on improving the fertilizer efficiency of the biological organic fertilizer. The use of the biological organic fertilizer of the present invention can promote plant growth and inhibit the invasion of pests and diseases on plants.
Claims
1. A high-efficiency bio-organic fertilizer, which is prepared by processing animal carcasses, characterized in that: The preparation method comprises the following steps: S1. Pretreatment: remove hair and bones from the animal carcass and freeze it at -20℃ to -25℃ for 5-7 days to completely freeze it; S2, crushing: cutting and crushing the frozen animal carcass to obtain frozen fragments; S3, inoculation: evenly mixing the frozen pieces with the composite starter to obtain mixed frozen pieces; The composite fermentation agent is composed of an enzyme composite preparation, a microbial composite bacterial agent, and nano-cerium oxide; according to the mass ratio, the enzyme composite preparation: the microbial composite bacterial agent: the nano-cerium oxide = 8-12: 80-90: 5-8; S4, microwave treatment: subjecting the mixed frozen pieces to microwave treatment for 10 min-15 min; the microwave power is 10 kW-30 kW, and the microwave frequency is 2400 MHz-2500 MHz; S5, puffing: after the microwave treatment, immediately pressurize to 5-8MPa and maintain for 20min-25min, then suddenly reduce the pressure to make it puffed; S6, fermentation: fermentation in a closed container at 30°C-50°C for 5d-6d to obtain fermented organic fertilizer; S7. Preparation: After mixing the fermented organic fertilizer with humic acid, decomposed crop straw and rice bran, 2%-3% of microbial flora is added to obtain a high-efficiency biological organic fertilizer; the microbial flora includes rhizobia, nitrogen-fixing bacteria, phosphate bacteria and silicate bacteria.
2. A highly efficient biological organic fertilizer as claimed in claim 1, characterized in that, In the step S2, the frozen animal carcass is cut and broken into pieces of 2-4 cm×2-4 cm to obtain frozen pieces.
3. A kind of high-efficiency biological organic fertilizer as claimed in claim 1, characterized in that, In step S3, the enzyme complex preparation is composed of collagenase, keratinase and lipase, and the mass ratio is collagenase: keratinase: lipase = 5-10: 20-30: 1-5.
4. A highly efficient biological organic fertilizer as claimed in claim 3, characterized in that, In step S3, the enzyme complex preparation is composed of collagenase, keratinase and lipase, and the mass ratio of collagenase: keratinase: lipase is 8:25:
3.
5. A highly efficient biological organic fertilizer as claimed in claim 1, characterized in that, In step S3, the microbial composite agent is bacillus, lactic acid bacteria, yeast, filamentous fungi, and actinomycetes, and the mass ratio is bacillus: lactic acid bacteria: yeast: filamentous fungi: actinomycetes = 4-6: 3-5: 2-4: 1-3: 3-6.
6. A highly efficient biological organic fertilizer as claimed in claim 5, characterized in that, In step S3, the microbial composite agent is bacillus, lactic acid bacteria, yeast, filamentous fungi, and actinomycetes, and the mass ratio of bacillus: lactic acid bacteria: yeast: filamentous fungi: actinomycetes is 5:4:2-4:2:
5.
7. A highly efficient bio-organic fertilizer as claimed in claim 1, characterized in that, In step S7, the mass ratio of the fermented organic fertilizer to the humic acid, the decomposed crop straw, and the rice bran is 6-10:2-4:3-6:1-3.
8. A highly efficient bio-organic fertilizer as claimed in claim 7, characterized in that, In step S7, the mass ratio of the rhizobium agent, the nitrogen-fixing bacteria agent, the phosphorus bacteria agent and the silicate bacteria agent is 1-3:1-2:2-4:1-3.
9. A highly efficient bio-organic fertilizer as claimed in any one of claims 1 to 9, characterized in that: The pH value of the high-efficiency biological organic fertilizer is 7.5-8.5, and the humidity is 20%-30%.
10. A high-efficiency biological organic fertilizer as claimed in claim 1, characterized in that: In step S7, the fermented organic fertilizer is mixed with humic acid, decomposed crop straw and rice bran, 2%-3% of microbial flora is added, and then chicken manure fermentation is added to obtain a high-efficiency biological organic fertilizer.
Citation Information
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