Organic water-soluble fertilizer of microbial enzymolysis humic acid as well as preparation method and application of organic water-soluble fertilizer
By optimizing the raw material ratio and enzymatic process of organic water-soluble fertilizers for enzymatic decomposition of microorganisms, and adding modified colloidal additives and polymer additives, the problems of fertilizer stability and preparation efficiency are solved, and high stability and excellentness are achieved soil effects.
Patent Information
- Application Number
- CN202411615768.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-12
- Publication Date
- 2025-02-18
- Estimated Expiration
- Not applicable · inactive patent
Smart Images
Figure BDA0005132181650000131
Abstract
Description
Technical Field
[0001] The present application relates to the field of fertilizers, and in particular to an organic water-soluble fertilizer produced by microbial enzymatic hydrolysis of humic acid, a preparation method and an application thereof. Background Art
[0002] As global agriculture develops towards green and sustainable development, organic water-soluble fertilizers are gaining more and more attention due to their environmental friendliness, high efficiency and safety. Although traditional chemical fertilizers can quickly replenish nutrients in the soil, long-term use can lead to problems such as soil compaction, decreased fertility and environmental pollution. In contrast, organic water-soluble fertilizers can effectively improve soil structure, promote plant root growth, and reduce negative impacts on the environment. Among the many types of organic water-soluble fertilizers, organic water-soluble fertilizers with microbial enzymatic hydrolysis of humic acid stand out for their unique functions and effects.
[0003] Organic water-soluble fertilizers with microbial enzymatic hydrolysis of humic acid use the enzyme system produced by specific microbial groups to carry out biotransformation of humic acid, breaking down its macromolecular structure into small molecules that are more easily absorbed by plants, such as humic acid, fulvic acid, etc. These small molecules can not only directly supply the carbon source and energy required by plants, but also significantly improve the availability of other nutrients in the soil, thereby promoting healthy plant growth.
[0004] However, although the organic water-soluble fertilizer of microbial enzymatic hydrolysis of humic acid has many advantages in theory, it still faces some challenges in the actual application process, such as the stability of the enzymatic hydrolysis products and water-soluble fertilizers, which involves the storage and transportation of fertilizers, and will directly affect the use cost and actual life of the fertilizers; and the overall enzymatic hydrolysis process is affected by the large differences in the composition of humic acid, and the actual preparation efficiency of the fertilizer is difficult to control. Therefore, in order to effectively solve the above problems, the present application provides an organic water-soluble fertilizer of biological enzymatic hydrolysis of humic acid and a preparation method. The organic water-soluble fertilizer prepared by the present application can not only achieve a stable humic acid enzymatic hydrolysis quality level, optimize the enzymatic hydrolysis process, and the organic water-soluble fertilizer finally prepared has good self-stability, can have excellent stability in the existing storage and transportation environment, reduce storage, transportation and use costs, and has very excellent market and application prospects. Summary of the invention
[0005] In order to solve the above problems, the first aspect of the present application provides an organic water-soluble fertilizer of humic acid hydrolyzed by microorganisms. The raw materials are, by mass: 80 to 100 parts of humic acid material, 10 to 15 parts of composite microbial composition, 15 to 20 parts of potassium humate, 2 to 4 parts of amino acids, 10 to 25 parts of functional additives, 10 to 15 parts of complex composition, 5 to 15 parts of metal compounds, and 120 to 160 parts of sterile deionized water.
[0006] As a preferred solution, the mass ratio of the humic acid material, the composite microbial composition and potassium fulvic acid is (85-95): (11-13): (16-18).
[0007] As a preferred solution, the mass ratio of the humic acid material, the composite microbial composition and potassium fulvic acid is (88-92): (12-13): (16-17).
[0008] As a preferred solution, the mass ratio of the amino acid, the functional additive and the complexing composition is (2-2.5): (18-25): (11-14).
[0009] As a preferred solution, the mass ratio of the amino acid, the functional additive and the complexing composition is (2.2-2.4): (19-22): (12-13).
[0010] As a preferred solution, the humic acid material is a composition of humic acid and ammonium humate.
[0011] As a preferred solution, the mass ratio of humic acid to ammonium humate is (8-10):(2-2.5).
[0012] As a preferred solution, the composite microbial composition is a composite microbial agent of Bacillus subtilis, Bacillus licheniformis, white rot fungi, Bacillus amyloliquefaciens and Lactobacillus brevis.
[0013] As a preferred solution, the mass ratio of Bacillus subtilis, Bacillus licheniformis, white rot fungus, Bacillus amyloliquefaciens and Lactobacillus brevis is (30-35): (20-25): (15-20): (10-15): (5-10).
[0014] As a preferred solution, the mass ratio of Bacillus subtilis, Bacillus licheniformis, white rot fungus, Bacillus amyloliquefaciens and Lactobacillus brevis is (32-34):(22-23):(18-20):(12-15):(8-10).
[0015] As a preferred embodiment, the amino acid is at least one of alanine, aspartic acid, lysine and tryptophan.
[0016] As a preferred embodiment, the amino acid is a combination of alanine and tryptophan.
[0017] As a preferred solution, the mass ratio of alanine to tryptophan is (3-4):(0.4-0.8).
[0018] As a preferred solution, the functional additive is a composition of a modified colloid additive and a polymer additive.
[0019] As a preferred solution, the mass ratio of the modified colloid auxiliary agent to the polymer auxiliary agent is (8-14): (3-8).
[0020] As a preferred solution, the mass ratio of the modified colloid auxiliary agent to the polymer auxiliary agent is (9-13):(4-6).
[0021] As a preferred solution, the preparation method of the modified colloid auxiliary agent includes the following steps: S1: adding amylose, amylopectin and xanthan gum to deionized water and mixing them evenly, then adding sodium nitrite, stirring at 0-5°C for 40-80 minutes, evacuating to vacuum, adding propyl acrylate, hydroxyethyl acrylate and acrylamide, then adding ammonia water to adjust the pH to 9-10, and adding initiator dropwise at 60-65°C; S2: maintaining the reaction container at 300-400rpm and 85-90°C for 3-4h, after the reaction is completed, adding triethanolamine, standing and keeping warm for 2-3h, then adding it to cool naturally to room temperature, and adjusting the pH to 7-7.5 with citric acid, after completion, the product is precipitated, centrifuged and filtered, washed with deionized water and dried.
[0022] As a preferred solution, the mass ratio of the amylose, amylopectin, xanthan gum and sodium nitrite is (3-5): (0.6-0.8): (8-10): (0.5-0.6).
[0023] As a preferred solution, the mass ratio of xanthan gum, propyl acrylate, hydroxyethyl acrylate and acrylamide is (8-10): (0.5-1): (3-4): (15-20).
[0024] As a preferred solution, the polymer auxiliary agent is a composition of lauryl alcohol polyoxyethylene ether and epoxy block polyether.
[0025] As a preferred solution, the mass ratio of lauryl alcohol polyoxyethylene ether to epoxy block polyether is (3-4): (1-1.2).
[0026] As a preferred solution, the hydroxyl value of the lauryl alcohol polyoxyethylene ether is 150-180 mgKOH / g.
[0027] As a preferred solution, the epoxy block polyether is a block copolyether of ethylene oxide-propylene oxide; the weight average molecular weight of the block copolyether of ethylene oxide-propylene oxide is 1200 to 2400 Da.
[0028] In the present application, the addition of the above-mentioned functional additives can effectively improve the self-stability and soil efficacy of organic water-soluble fertilizers. The combined action of the added polymer additives and modified colloid additives can form a dense interface film with the polymer additives as the main component in the water-soluble system, and the presence of the film layer can effectively improve the free resistance between fertilizer molecules in the aqueous system through the help of the modified colloid additives, and a large amount of molecular weights distributed by the modified colloid additives form a stable consistency region in a stable system, in which the long molecular chains and multi-branched connections of amylopectin and amylopectin greatly reduce the contact probability of active molecules and groups in the active state, reduce the degree of collision, and then greatly reduce the probability of its internal active reaction, so as to maintain the high stability of the internal system during long-term storage and transportation, reduce precipitation, stratification and a large number of flocculation phenomena, and effectively reduce the storage and transportation costs of fertilizers.
[0029] As a preferred solution, the complex composition is a polyglutamic acid complex composition.
[0030] As a preferred solution, the preparation method of the polyglutamic acid complex composition comprises the following steps: S1: dissolving ferrous sulfate, copper sulfate and manganese sulfate in deionized water to form a salt solution, then adding polyglutamic acid and mixing while stirring to mix evenly, and adding sodium hydroxide to adjust the pH to 7.5-8; S2: heating to 65-70°C, then keeping the temperature for reaction for 2-2.5h, and controlling the pH to 7.5-8 by adding sodium hydroxide during the reaction to obtain a product mixed solution; S3: adding a small amount of hydrochloric acid to the product mixed solution to adjust the pH to 7, and standing for 1-2h to allow precipitation to form and aggregate, thereby obtaining the polyglutamic acid complex composition.
[0031] As a preferred solution, the mass ratio of ferrous sulfate, cupric sulfate, manganese sulfate and polyglutamic acid is (0.8-1): (1.2-1.5): (0.2-0.4): (4-4.5).
[0032] As a preferred solution, the metal compound is at least one of zinc sulfate, magnesium sulfate, magnesium nitrate, calcium chloride, copper sulfate and calcium nitrate.
[0033] As a preferred solution, the metal compound is a combination of zinc sulfate, magnesium sulfate, calcium chloride and copper sulfate.
[0034] As a preferred solution, the mass ratio of zinc sulfate, magnesium sulfate, calcium chloride and copper sulfate is (1-1.5): (0.4-0.6): (2.5-3): (1.8-2).
[0035] The second aspect of the present application provides a method for preparing the above-mentioned organic water-soluble fertilizer of microbial enzymatic humic acid, which specifically includes the following steps: S1: crushing and sieving the humic acid material to an average particle size of ≤1mm, then adding the sieved humic acid material to an enzymatic hydrolysis tank under sterile conditions, adding sterile deionized water and then adding a composite microbial composition, controlling the temperature to 30-35°C and storing for 3-5 days, removing the solid residue after completion to obtain a humic acid enzymatic hydrolyzate; S2: adding the remaining raw materials to the humic acid enzymatic hydrolyzate in sequence, the addition of each raw material is accompanied by stirring at 60-80rpm for 30-40min, after completion, adding sterile water, adding to a clean container, sealing and storing, and obtaining.
[0036] The third aspect of the present application provides an organic water-soluble fertilizer produced by the enzymatic hydrolysis of humic acid by microorganisms for use in agricultural planting, fruit tree cultivation, flower planting and landscaping.
[0037] The beneficial effects of this application are:
[0038] 1. The organic water-soluble fertilizer of humic acid hydrolyzed by microorganisms provided in the present application can not only achieve a stable quality level of humic acid hydrolysis and optimize the hydrolysis process, but also the organic water-soluble fertilizer finally obtained has good self-stability, can have excellent stability in the existing storage and transportation environment, reduce storage, transportation and use costs, and has very excellent market and application prospects.
[0039] 2. The present application provides an organic water-soluble fertilizer of humic acid hydrolyzed by microorganisms, wherein the added functional additives can effectively improve the self-stability of the organic water-soluble fertilizer and its soil efficacy; the combined action of the added polymer additives and modified colloid additives can form a dense interfacial film with the polymer additives as the main component in the water-soluble system, and the presence of the film layer can effectively increase the free resistance between fertilizer molecules in the aqueous system with the help of the modified colloid additives, and form a stable consistency region in the stable system through the large amount of molecular weight emitted by the modified colloid additives, in which the long molecular chains and multi-branched connections of amylopectin and amylopectin greatly reduce the contact probability of active molecules and groups in the active state, reduce the degree of collision, and thus greatly reduce the probability of internal active reactions, thereby maintaining the high stability of the internal system during long-term storage and transportation, reducing precipitation, stratification and a large number of flocculation phenomena, and effectively reducing the storage and transportation costs of the fertilizer.
[0040] 3. The organic water-soluble fertilizer provided in the present application is a microbial enzymatic hydrolysis of humic acid. The raw materials for its preparation are simple and easy to obtain, the preparation steps are simple, and the final organic water-soluble fertilizer also has good soil improvement and water retention performance, which can greatly improve the water retention rate of the soil, thereby optimizing the soil structure and improving the soil planting efficiency. DETAILED DESCRIPTION
[0041] The following will further explain and demonstrate the technical solutions in the above invention content of this application in the form of specific implementation schemes. The following embodiments are only practical examples used to illustrate and explain the contents of the technical solutions in the specification, and should not limit the scope of the claims to be protected by this application. All technical products based on the technical solutions described in the invention content of this application should be included in the scope to be protected by this application.
[0042] In the following examples, unless otherwise specified, the raw materials are all commercially available products, or can be prepared by methods well known to those skilled in the art.
[0043] Example 1
[0044] Example 1 The first aspect provides an organic water-soluble fertilizer of humic acid hydrolyzed by microorganisms, wherein the raw materials are, by mass: 90.5 parts of humic acid material, 12.8 parts of composite microbial composition, 16.6 parts of potassium humate, 2.2 parts of amino acid, 21.5 parts of functional additives, 12.2 parts of complex composition, 9.5 parts of metal compound, and 145 parts of sterile deionized water.
[0045] The humic acid material is a composition of humic acid and ammonium humate, and the mass ratio of the two is 9.5:2.3; the humic acid was purchased from Xinjiang Shuanglong Humic Acid Co., Ltd.
[0046] The composite microbial composition is a composite microbial agent of Bacillus subtilis, Bacillus licheniformis, white rot fungus, Bacillus amyloliquefaciens and Lactobacillus brevis, with a mass ratio of 33.5:22:19:13.5:9.
[0047] The amino acid is a combination of alanine and tryptophan, and the mass ratio of the two is 3.6:0.5.
[0048] The functional additive is a composite of a modified colloid additive and a polymer additive, and the mass ratio of the two is 11:5.8.
[0049] The preparation method of the modified colloid auxiliary agent comprises the following steps, calculated by mass: S1: adding 4.2 parts of amylose, 0.75 parts of amylopectin and 10 parts of xanthan gum to 200 parts of deionized water and mixing them evenly, then adding 0.55 parts of sodium nitrite, stirring at 4°C for 60 minutes, evacuating to vacuum, adding 0.8 parts of propyl acrylate, 3.2 parts of hydroxyethyl acrylate and 18.8 parts of acrylamide, then adding ammonia water to adjust the pH to 10, and adding an initiator (a total of 5 parts of potassium persulfate aqueous solution, including 0.2 parts of potassium persulfate) dropwise at 65°C; S2: maintaining the reaction container at 350rpm, keeping the temperature at 85°C for 3.5 hours, after the reaction is completed, adding 0.5 parts of triethanolamine, standing and keeping the temperature for 2.5 hours, then adding it to cool naturally to room temperature, and adjusting the pH to 7.2 with citric acid, after completion, the product is precipitated, centrifuged and filtered, washed with deionized water and dried.
[0050] Xanthan gum was purchased from Anhui Zhonghong Bioengineering Co., Ltd. as a food-grade product.
[0051] The polymer auxiliary agent is a composition of lauryl alcohol polyoxyethylene ether and epoxy block polyether, and the mass ratio of the two is 3.2:1.
[0052] The hydroxyl value of lauryl alcohol polyoxyethylene ether is 155 mgKOH / g, and it was purchased from Hai'an Guoyun Chemical Co., Ltd. as a MOA-4 product.
[0053] The epoxy block polyether is a block copolyether of ethylene oxide-propylene oxide, has a weight average molecular weight of 1900 Da, and is purchased from the L42 block copolyether product of ethylene oxide-propylene oxide sold by BASF.
[0054] The complex composition is a polyglutamic acid complex composition; the preparation method of the polyglutamic acid complex composition comprises the following steps, calculated by mass: S1: dissolving 0.85 parts of ferrous sulfate, 1.4 parts of copper sulfate and 0.25 parts of manganese sulfate in 100 parts of deionized water to form a salt solution, then adding 4.4 parts of polyglutamic acid and stirring while adding to mix evenly, adding sodium hydroxide to adjust the pH to 7.8; S2: heating to 70°C, then keeping the temperature for reaction for 2 hours, and controlling the pH to 7.5-8 by adding sodium hydroxide during the reaction process to obtain a product mixed solution; S3: adding a small amount of hydrochloric acid to the product mixed solution to adjust the pH to 7, and standing for 1.5 hours to allow precipitation to form and aggregate, so as to obtain the polyglutamic acid complex composition.
[0055] Polyglutamic acid was purchased from food-grade polyglutamic acid products sold by Guangdong Mingtong Biotechnology Co., Ltd.
[0056] The metal compound is a composition of zinc sulfate, magnesium sulfate, calcium chloride and copper sulfate, with a mass ratio of 1.2:0.5:2.8:2.
[0057] The second aspect of the present embodiment provides a method for preparing the above-mentioned organic water-soluble fertilizer of microbial enzymatic humic acid, which specifically includes the following steps: S1: crushing and sieving the humic acid material to an average particle size of ≤1 mm, then adding the sifted humic acid material to an enzymatic hydrolysis tank under sterile conditions, adding sterile deionized water and then adding a composite microbial composition, controlling the temperature to 32°C and storing for 4 days, and removing the solid residue after completion to obtain a humic acid enzymatic hydrolyzate; S2: adding the remaining raw materials to the humic acid enzymatic hydrolyzate in sequence, and adding each raw material while stirring at 70rpm for 40min. After completion, adding sterile water, adding to a clean container, and sealing and storing to obtain the product.
[0058] Example 2
[0059] The specific implementation of this embodiment is basically the same as that of Example 1, except that the organic water-soluble fertilizer of microbial enzymatic humic acid comprises, by weight, 85 parts of humic acid material, 13 parts of composite microbial composition, 18 parts of potassium humate, 2 parts of amino acid, 24.8 parts of functional additive, 13.6 parts of complex composition, 9.5 parts of metal compound and 145 parts of sterile deionized water.
[0060] Example 3
[0061] The specific implementation of this embodiment is basically the same as that of Example 1, except that the organic water-soluble fertilizer of microbial enzymatic humic acid comprises, by weight, 95 parts of humic acid material, 11 parts of composite microbial composition, 16 parts of potassium humate, 2.5 parts of amino acids, 18.8 parts of functional additives, 11.2 parts of complex composition, 9.5 parts of metal compounds, and 140 parts of sterile deionized water.
[0062] Comparative Example 1
[0063] The specific implementation manner of this comparative example is basically the same as that of Example 1, except that the organic water-soluble fertilizer of microbial enzymatic humic acid, in parts by mass, comprises the following raw materials: 90 parts of humic acid material, 5 parts of composite microbial composition, 25 parts of potassium humate, 2 parts of amino acid, 24.8 parts of functional additive, 8.2 parts of complex composition, 9.5 parts of metal compound and 125 parts of sterile deionized water.
[0064] Comparative Example 2
[0065] The specific implementation method of this comparative example is basically the same as that of Example 1, except that the organic water-soluble fertilizer of microbial enzymatic humic acid, in parts by mass, comprises the following raw materials: 60 parts of humic acid material, 22 parts of composite microbial composition, 18 parts of potassium humate, 2 parts of amino acid, 24.8 parts of functional additive, 25.6 parts of complex composition, 9.5 parts of metal compound and 133 parts of sterile deionized water.
[0066] Comparative Example 3
[0067] The specific implementation of this comparative example is basically the same as that of Example 1, except that the functional additive is a composition of a modified colloid additive and a polymer additive, and the mass ratio of the two is 15:1.8.
[0068] Comparative Example 4
[0069] The specific implementation of this comparative example is basically the same as that of Example 1, except that the polymer auxiliary agent is a composition of lauryl alcohol polyoxyethylene ether and epoxy block polyether, and the mass ratio of the two is 1:2.
[0070] Comparative Example 5
[0071] The specific implementation of this comparative example is basically the same as that of Example 1, except that: the preparation method of the modified colloid auxiliary agent comprises the following steps, calculated by mass: S1: 5.5 parts of amylose, 0.15 parts of amylopectin and 25 parts of xanthan gum are added to 200 parts of deionized water and mixed evenly, then 0.55 parts of sodium nitrite are added, stirred at 4°C for 60 minutes, evacuated to vacuum, 0.8 parts of propyl acrylate, 3.2 parts of hydroxyethyl acrylate and 18.8 parts of acrylamide are added, then ammonia water is added to adjust the pH to 10, and an initiator (a total of 5 parts of potassium persulfate aqueous solution, including 0.2 parts of potassium persulfate) is added dropwise at 65°C; S2: the reaction vessel is maintained at 350rpm and kept warm at 85°C for 3.5 hours. After the reaction is completed, 0.5 parts of triethanolamine are added, and the temperature is naturally cooled to room temperature after standing and keeping warm for 2.5 hours, and citric acid is added to adjust the pH to 7.2. After completion, the product is precipitated, centrifuged, filtered, washed with deionized water, and dried.
[0072] Comparative Example 6
[0073] The specific implementation of this comparative example is basically the same as that of Example 1, except that: the preparation method of the modified colloid auxiliary agent comprises the following steps, calculated by mass: S1: 4.2 parts of amylose, 0.75 parts of amylopectin and 10 parts of xanthan gum are added to 200 parts of deionized water and mixed evenly, then 0.55 parts of sodium nitrite are added, stirred at 4°C for 60 minutes, evacuated to vacuum, 0.2 parts of propyl acrylate, 1.1 parts of hydroxyethyl acrylate and 30 parts of acrylamide are added, then ammonia water is added to adjust the pH to 10, and an initiator (a total of 5 parts of potassium persulfate aqueous solution, including 0.2 parts of potassium persulfate) is added dropwise at 65°C; S2: the reaction vessel is maintained at 350rpm and kept warm at 85°C for 3.5 hours. After the reaction is completed, 0.5 parts of triethanolamine are added, and the temperature is naturally cooled to room temperature after standing and keeping warm for 2.5 hours, and citric acid is added to adjust the pH to 7.2. After completion, the product is precipitated, centrifuged, filtered, washed with deionized water, and dried.
[0074] Performance Evaluation
[0075] Soil water retention rate test: The eggplant planting plot in Shouguang City, Shandong Province was divided into 9 areas of 3 mu. During the test, the soil in the 9 planting areas was plowed 6-8 cm along the planting rows, and the fertilizers prepared in the above embodiments and comparative examples were sprayed respectively and then plowed into the soil again. The application rate was 20 kg / mu. The water retention rate of the soil on the day of fertilization was tested. After 14 days of normal maintenance, the water retention rate of the soil was tested again, and the percentage increase of the water retention rate of the soil before and after was calculated. The measured value was taken as the average value of 10 tests and recorded in Table 1.
[0076] Thermal stability test: The fertilizers prepared in the embodiments and comparative examples were stored in a constant temperature box at 60±2°C and a relative humidity of 75±2% for 14 days, and the total mass decrease percentage of the fertilizer after 14 days of storage was recorded. The total mass decrease percentage % = {1-(mass after test / mass before test)} × 100%, and the average value of 10 measured values was recorded in Table 1.
[0077] Storage stability test: The fertilizers prepared in the embodiments and comparative examples were fixedly stored at 30±2°C and a relative humidity of 50±2%. After 60 days of storage, the fertilizers were observed to see whether there was obvious deterioration, surface suspended impurities, and phase separation. If there was, the fertilizers were unqualified, otherwise, they were qualified. In this test, 50 samples were tested in parallel for each embodiment and comparative example group, and the qualified rate of the samples among the 50 samples was recorded. The results are recorded in Table 1.
[0078] Table 1 Performance test results
[0079]
[0080] It can be seen from the examples and comparative examples of the present application and the data results in Table 1 that Examples 1 to 3 of the present application have obvious advantages over Comparative Examples 1 to 6 in terms of fertilizer efficacy, soil water retention and transportation / storage stability. This is mainly due to the effect of the modified functional additives and other matching technical solutions specified in the present application. Comparative Examples 1 to 6 did not adopt the technical solution specified in the present application, resulting in obvious disadvantages in the above performance tests. This further proves the necessity of the technical solution specified in the present application for the technical effect of the present application and solving technical problems.
Claims
1. An organic water-soluble fertilizer of humic acid hydrolyzed by microorganisms, characterized in that: The raw materials are as follows, by weight: 80-100 parts of humic acid material, 10-15 parts of composite microbial composition, 15-20 parts of potassium fulvic acid, 2-4 parts of amino acid, 10-25 parts of functional additive, 10-15 parts of complex composition, 5-15 parts of metal compound, and 120-160 parts of sterile deionized water; The humic acid material is a composition of humic acid and ammonium humate; the mass ratio of the humic acid to the ammonium humate is (8-10): (2-2.5); The composite microbial composition is a composite microbial agent of Bacillus subtilis, Bacillus licheniformis, white rot fungus, Bacillus amyloliquefaciens and Lactobacillus brevis; the mass ratio of Bacillus subtilis, Bacillus licheniformis, white rot fungus, Bacillus amyloliquefaciens and Lactobacillus brevis is (30-35): (20-25): (15-20): (10-15): (5-10); The amino acid is at least one of alanine, aspartic acid, lysine and tryptophan; The functional additive is a composition of a modified colloid additive and a polymer additive; the mass ratio of the modified colloid additive to the polymer additive is (8-14): (3-8); Preparation method of the modified colloid auxiliary agent The following steps are involved: S1: Add amylose, amylopectin and xanthan gum to deionized water and mix well, then add sodium nitrite, stir at 0-5°C for 40-80min, evacuate to vacuum, add propyl acrylate, hydroxyethyl acrylate and acrylamide, then add ammonia water to adjust the pH to 9-10, and add initiator dropwise at 60-65°C; S2: Keep the reaction vessel at 300-400rpm and 85-90°C for 3-4h. After the reaction is completed, add triethanolamine, keep it still for 2-3h, then add it to cool naturally to room temperature, and adjust the pH to 7-7.5 with citric acid. After completion, wait for the product to precipitate, centrifuge and filter, wash with deionized water and dry; The mass ratio of the amylose, amylopectin, xanthan gum and sodium nitrite is (3-5): (0.6-0.8): (8-10): (0.5-0.6); The mass ratio of xanthan gum, propyl acrylate, hydroxyethyl acrylate and acrylamide is (8-10): (0.5-1): (3-4): (15-20).
2. The organic water-soluble fertilizer of humic acid hydrolyzed by microorganisms according to claim 1, characterized in that: The mass ratio of the humic acid material, the composite microbial composition and potassium fulvic acid is (85-95): (11-13): (16-18).
3. The organic water-soluble fertilizer of humic acid hydrolyzed by microorganisms according to claim 2, characterized in that: The mass ratio of the amino acid, the functional auxiliary agent and the complexing composition is (2-2.5): (18-25): (11-14).
4. The organic water-soluble fertilizer of humic acid hydrolyzed by microorganisms according to claim 3, characterized in that: The amino acid is a combination of alanine and tryptophan; the mass ratio of alanine to tryptophan is (3-4): (0.4-0.8).
5. The organic water-soluble fertilizer of humic acid hydrolyzed by microorganisms according to claim 4, characterized in that: The polymer auxiliary agent is a composition of lauryl alcohol polyoxyethylene ether and epoxy block polyether; the mass ratio of the lauryl alcohol polyoxyethylene ether and the epoxy block polyether is (3-4): (1-1.2).
6. The organic water-soluble fertilizer of humic acid hydrolyzed by microorganisms according to claim 5, characterized in that: The epoxy block polyether is a block copolyether of ethylene oxide-propylene oxide; the weight average molecular weight of the block copolyether of ethylene oxide-propylene oxide is 1200-2400 Da.
7. The organic water-soluble fertilizer of humic acid hydrolyzed by microorganisms according to claim 6, characterized in that: The complex composition is a polyglutamic acid complex composition; the preparation method of the polyglutamic acid complex composition comprises the following steps: S1: dissolving ferrous sulfate, copper sulfate and manganese sulfate in deionized water to form a salt solution, then adding polyglutamic acid and mixing while stirring to mix evenly, adding sodium hydroxide to adjust the pH value to 7.5-8; S2: heating to 65-70°C, then keeping the temperature for reaction for 2-2.5h, and controlling the pH value to 7.5-8 by adding sodium hydroxide during the reaction process to obtain a product mixed solution; S3: adding a small amount of hydrochloric acid to the product mixed solution to adjust the pH value to 7, and standing for 1-2h to allow precipitation to form and aggregate, thereby obtaining the polyglutamic acid complex composition.
8. The organic water-soluble fertilizer of humic acid hydrolyzed by microorganisms according to claim 7, characterized in that: The metal compound is at least one of zinc sulfate, magnesium sulfate, magnesium nitrate, calcium chloride, copper sulfate and calcium nitrate.
9. A method for preparing an organic water-soluble fertilizer of humic acid hydrolyzed by microorganisms according to any one of claims 1 to 8, characterized in that: The specific steps include: S1: Grind and sieve the humic acid material to an average particle size of ≤1 mm, then add the sieved humic acid material into an enzymolysis tank under sterile conditions, add sterile deionized water, and then add the composite microbial composition, control the temperature at 30-35°C and store for 3-5 days, remove the solid residue after completion, and obtain a humic acid enzymolysis solution; S2: Add the remaining raw materials to the humic acid enzymolysis solution in sequence, and the addition of each raw material is accompanied by stirring at 60-80rpm for 30-40min. After completion, add sterile water, add to a clean container, and seal and store.
10. Use of the organic water-soluble fertilizer obtained by enzymolysis of humic acid by microorganisms according to any one of claims 1 to 8 in agricultural planting, fruit tree cultivation, flower planting and landscaping.