Device and method for evaluating developmental toxicity of high-concentration cyanotoxin based on fish embryos

Abstract

The present invention relates to a device and method for evaluating the developmental toxicity of high-concentration cyanotoxin based on fish embryos, and relates to the technical field of pollutant toxicity analysis. The device structure includes a machine base and an electric guide rail mechanism I; a carrier frame slidably arranged on the electric guide rail mechanism I, an active oscillation seat is arranged on the carrier frame, and a plurality of multi-well plates for placing fish embryos are arranged on the active oscillation seat. A lighting structure is arranged at a position below the carrier frame and at the position of the active oscillation seat; a liquid injection unit group and an incubation chamber, the liquid injection unit group is used to inject cyanotoxin working solutions with different high concentrations into a plurality of multi-well plates, and an observation unit group is arranged at the top of the incubation chamber; and an adjustable light-shielding unit group arranged between the lighting structure and the multi-well plates. The evaluation device provided by the present invention is convenient to use, reduces the manual labor intensity, improves the work efficiency, and is convenient for large-scale and high-efficiency evaluation and analysis of the developmental toxicity of high-concentration cyanotoxin to fish embryos.

Description

Technical Field

[0001] The present invention belongs to the technical field of pollutant toxicity analysis, and particularly relates to a device and method for evaluating the developmental toxicity of high-concentration cyanotoxin based on fish embryos. Background Art

[0002] As a new pollutant, cyanotoxin (CYN) has been widely present in aquatic ecosystems and has the characteristic of being difficult to degrade. At the same time, cyanotoxin poses a potential threat to the ecological environment and human health through various channels such as drinking water, aquatic products, and water-based recreational activities. Therefore, exploring the toxic effects and potential mechanisms of cyanotoxin is of research significance for understanding and preventing cyanotoxin.

[0003] To explore the toxic effects and potential mechanisms of cyanotoxin, the fertilized fish embryos 4 hours after fertilization can be exposed to different high concentrations of CYN for a certain incubation period to evaluate the effects of CYN on the early developmental toxicity, behavioral toxicity, and neurotoxicity of zebrafish, so as to further enrich the data on the toxicity of cyanotoxin to aquatic organisms. Among them, the evaluation of early developmental toxicity is mostly carried out from aspects such as recording the mortality rate, hatching rate, and malformation incidence rate of fish embryos. At the same time, dead or malformed embryos need to be removed in time during the recording process to ensure the accuracy of the experiment.

[0004] Currently, the evaluation process of early developmental toxicity is usually carried out manually through a series of cumbersome steps, including injecting different high-concentration CYN solutions into the samples, transferring the samples to the incubator for incubation, and regular recording during the incubation process. The operation and observation are rather troublesome, increasing the manual labor intensity and reducing the work efficiency, which is not conducive to efficiently evaluating the developmental toxicity of high-concentration cyanotoxin. For this reason, we propose a device and method for evaluating the developmental toxicity of high-concentration cyanotoxin based on fish embryos. Summary of the Invention

[0005] The purpose of the present invention is to provide a device and method for evaluating the developmental toxicity of high-concentration cyanotoxin based on fish embryos to solve the above problems.

[0006] The present invention realizes the above purpose through the following technical solutions:

[0007] As the first aspect of the present invention, a device for evaluating the developmental toxicity of high-concentration cyanotoxin based on fish embryos is provided, including a machine base, and further including an electric guide rail mechanism 1 extending along the length direction of the machine base into the incubation chamber;

[0008] A carrier mounted slidably on an electric guide rail mechanism. An active oscillation seat is provided on the carrier, and a plurality of porous plates for placing fish embryos are provided on the active oscillation seat. A lighting structure is provided at a position below the active oscillation seat of the carrier to provide the light conditions required for fish embryos.

[0009] A liquid injection unit and an incubation chamber extending along the length direction of the machine base. The liquid injection unit is used to inject working fluids of different high concentrations of cyanotoxin into a plurality of porous plates, and an observation unit is provided at the top of the incubation chamber.

[0010] And an adjustable light-shielding unit provided between the lighting structure and the porous plates to adjust the contrast between the lower part of the porous plates and the environment, facilitating the observation of the development of fish embryos through the observation unit.

[0011] As a further optimized solution of the present invention, the carrier includes a gantry 1, a panel provided on the gantry 1, elastic telescopic members provided at both ends of the active oscillation seat and movably inserted into the panel, and a driving oscillation unit provided on the gantry 1 for driving the active oscillation seat to oscillate.

[0012] As a further optimized solution of the present invention, the driving oscillation unit includes a transmission rod axially connected to the gantry 1 and an eccentric connecting member. The transmission rod rotates synchronously with the eccentric connecting member through a transmission member. When the eccentric connecting member rotates, its outer end intermittently and periodically pushes the active oscillation seat to realize the oscillating swing of the active oscillation seat.

[0013] As a further optimized solution of the present invention, hollow grooves are provided at positions corresponding to the holes of the porous plates on the active oscillation seat. The structure of the adjustable light-shielding unit includes a driving roller, a winding roller, and a light-shielding cloth provided between the driving roller and the winding roller. The light-shielding cloth has hollow parts.

[0014] As a further optimized solution of the present invention, a multi-directional adjustment structure is provided at the top of the incubation chamber for mounting the observation unit. The structure of the multi-directional adjustment structure includes an elastic membrane provided at the top end of the incubation chamber, a mounting seat for fixing the observation unit provided on the elastic membrane, an annular slide rail provided in the incubation chamber, a sliding block movably provided in the annular slide rail, a telescopic rod with one end hinged to the outside of the sliding block, and a hinge seat hinged to the other end of the telescopic rod. The hinge seat is hinged to one end of the observation unit extending into the incubation chamber.

[0015] As a further optimized solution of the present invention, the structure of the observation unit includes a cylinder connected to the multi-directional adjustment structure, an observation eyepiece provided at one end of the cylinder located outside the incubation chamber, and a convex lens provided at one end of the cylinder located inside the incubation chamber.

[0016] As a further optimized solution of the present invention, the structure of the liquid injection unit includes a second gantry, several pumping and liquid injection cylinders are arranged at the top of the second gantry, a liquid injection head connected to the pumping and liquid injection cylinder through a delivery pipe, a cross plate connected to the liquid injection head, a positioning rod movably sleeved at the lower end of the second gantry and the cross plate, a pair of electric guide rail mechanisms two arranged in the length direction of the second gantry, and a horizontal axis arranged in front of the delivery pipe and driven to move by the electric guide rail mechanism two. A travel switch electrically connected to the electric guide rail mechanism two is provided at the bottom end of the positioning rod, and a convex platform with a height equal to the placement height of the porous plate is provided at the position corresponding to the positioning rod on the carrier.

[0017] As a further optimized solution of the present invention, an operation glove is installed on one side of the hatching room relative to the liquid injection unit, and a tool box is slidably arranged in the hatching room.

[0018] As the second aspect of the present invention, there is also provided an evaluation method for the developmental toxicity of high-concentration cyanotoxin based on fish embryos by using the evaluation device as described in any one of the above, including the following steps:

[0019] Step 1: Select several normally developing zebrafish embryos, place them in the holes of several porous plates in equal numbers respectively, and then place the several porous plates in the movable oscillation seats on the carrier.

[0020] Step 2: Drive the porous plate on the carrier to move to the liquid injection unit through the electric guide rail mechanism one, inject equal volumes of cyanotoxin working solutions with different concentrations into the several porous plates respectively through the liquid injection unit, and make the zebrafish embryos and the cyanotoxin working solutions mix evenly by controlling the oscillation of the movable oscillation seat.

[0021] Step 3: Drive the porous plate on the carrier to move into the hatching room through the electric guide rail mechanism one, and provide light conditions through the lighting structure, and incubate the zebrafish embryos in the hatching room under the set hatching time.

[0022] Step 4: During the incubation process, observe the growth status of the zebrafish embryos through the observation unit, regularly record the number of dead, hatched and deformed zebrafish embryos, and use the mortality rate, hatching rate and malformation incidence rate of the zebrafish embryos as evaluation indicators to evaluate the developmental toxicity of cyanotoxin to the zebrafish embryos.

[0023] The beneficial effects of the present invention are as follows:

[0024] (1) The present invention transports a load rack through an electric guide rail mechanism I, so that the fish embryos in the porous plate placed on the load rack are sent to the hatching chamber for hatching. During the transportation process, different high-concentration CYN solutions are automatically injected into the porous plates on the load rack through a liquid injection mechanism. During the hatching process, the development of the fish embryos in each porous plate in the hatching chamber is observed through an observation unit, and the mortality rate, hatching rate and malformation rate of the embryos are recorded. The device is easy to use, reduces the manual labor intensity, improves the work efficiency, and is convenient for large-scale and high-efficiency evaluation and analysis of the toxicity of high-concentration cyanotoxin to the development of fish embryos.

[0025] (2) The present invention is provided with an adjustable shading unit that can adjust the contrast between the lower part of the porous plate and the environment, which is convenient for experimental operators to observe the development of fish embryos and has good operation effect. In addition, by setting a multi-directional adjustment structure, the observation unit can be further flexibly adjusted according to the usage requirements of experimental operators. Compared with a multi-axis manipulator, the multi-directional adjustment structure has a simple structure, a small floor area, low use and maintenance costs, and good use effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 is a three-dimensional view of the overall structure of the device provided by the present invention;

[0027] Figure 2 is a schematic structural diagram of the load rack provided by the present invention;

[0028] Figure 3 is a schematic internal structure diagram of the hatching chamber provided by the present invention;

[0029] Figure 4 is a three-dimensional view of the structure of the light-shielding cloth provided by the present invention;

[0030] Figure 5 is a schematic overall structure diagram of the liquid injection unit provided by the present invention;

[0031] In the figure: 1, machine base; 2, electric guide rail mechanism I; 3, load rack; 31, gantry I; 32, panel; 33, movable oscillation seat; 34, elastic telescopic member; 4, liquid injection unit; 41, gantry II; 42, pumping and injecting cylinder; 43, injection head; 44, positioning rod; 45, cross plate; 46, travel switch; 47, electric guide rail mechanism II; 48, horizontal axis; 5, hatching chamber; 6, observation unit; 61, observation eye mask; 62, cylinder body; 63, convex lens; 7, porous plate; 8, multi-directional adjustment structure; 81, annular slide rail; 82, elastic membrane; 83, mounting seat; 84, hinge seat; 85, telescopic rod; 86, sliding block; 9, adjustable shading unit; 91, driving roller; 92, light-shielding cloth; 93, winding roller; 10, driving oscillation unit; 101, transmission rod; 102, transmission member; 103, eccentric connecting member; 11, operating glove; 12, tool box; 13, convex platform. Detailed implementation mode

[0032] The present application will be further described in detail below with reference to the accompanying drawings. It is necessary to point out here that the following specific implementation modes are only used to further illustrate the present application and cannot be understood as limiting the protection scope of the present application. Those skilled in the art can make some non-essential improvements and adjustments to the present application based on the above application content.

[0033] Example 1

[0034] This example provides a device for evaluating the developmental toxicity of high-concentration cyanotoxin based on fish embryos. After injecting different high-concentration cyanotoxins into the culture solution of fish embryos, the treated fish embryos are hatched. The hatching temperature is controlled at 28±1°C, and the light cycle is set to 14 hours of light and 10 hours of dark period. During the test set period, the mortality rate, hatching rate, and malformation incidence rate of the embryos are regularly recorded, and the developmental toxicity of cyanotoxin is evaluated based on the statistical data.

[0035] As Figure 1 shown, the structure of the device includes a machine base 1, and also includes an electric guide rail mechanism 2 extending along the length direction of the machine base 1 into the hatching chamber 5. The electric guide rail mechanism 2 is a conventional structure in the art, including a guide rail, a slider, a motor, etc., and will not be described in detail here.

[0036] A carrier 3 slidably arranged on the electric guide rail mechanism 2. The carrier 3 is provided with a movable oscillation seat 33, and the movable oscillation seat 33 is provided with a plurality of porous plates 7 for placing fish embryos. In this example, the movable oscillation seat 33 is preferably two groups. The power source for driving the movable oscillation seat 33 to move relative to the carrier 3 can be manually pushed by the test operator or an electric drive device such as a linear motor installed on the movable oscillation seat 33.

[0037] The fish embryos placed in the porous plate 7 are preferably embryos of the mature aquatic biological model - zebrafish, preferably a 6-well plate. As Figure 2 shown, the shape of the porous plate 7 is designed to match the shape of the movable oscillation seat 33. In addition, a lighting structure is provided at the position below the carrier 3 where the movable oscillation seat 33 is located, for providing the lighting conditions required for the hatching of fish embryos.

[0038] A liquid injection unit 4 and an incubation chamber 5 are arranged to extend along the length direction of the machine base 1. The liquid injection unit 4 is used to inject cyanotoxin working solutions with different high concentrations into a plurality of porous plates 7. A notch is left on one side of the incubation chamber 5 facing the liquid injection unit 4 to facilitate the electric guide rail mechanism 1 to push the carrier rack 3 into the incubation chamber 5. In addition, to ensure the structural seal of the incubation chamber 5, one side of the carrier rack 3 is designed with a door panel structure adapted to the notch. An observation unit 6 is provided at the top of the incubation chamber 5. The observation unit 6 facilitates the test operator to regularly record the mortality rate, hatching rate, and malformation incidence rate of fish embryos during the test. In addition, an operation glove 11 is installed on one side of the incubation chamber 5 opposite to the liquid injection unit 4, and a tool box 12 is slidably arranged in the incubation chamber 5. During the experiment, while regularly recording, it is necessary to remove dead or malformed embryos to maintain the accuracy of the experiment. The test operator can use relevant operation tools such as a cell clamp to extract and remove the dead embryos through the operation glove 11. The tool box 12 can store relevant tools or the extracted dead or malformed embryos.

[0039] The structure of the device further includes an adjustment light-shielding unit 9 arranged between the lighting structure and the porous plate 7, which is used to adjust the contrast between the lower part of the porous plate 7 and the environment, so as to facilitate observing the development of fish embryos through the observation unit 6. A hollow groove is provided at the position corresponding to the holes of the porous plate 7 on the movable oscillation seat 33. The structure of the adjustment light-shielding unit 9 includes a driving roller 91, a winding roller 93, and a light-shielding cloth 92 arranged between the driving roller 91 and the winding roller 93. The light-shielding cloth 92 has a hollow part (as Figure 4 shown). The non-hollow part of the light-shielding cloth 92 is preferably set to black. Since the fish embryos are transparent when alive and the dead embryos are partially or completely milky white, the black background is more likely to detect dead embryos or embryos with abnormal development or malformations. Driven by an external driving source, preferably a driving motor, the driving roller 91 pulls the light-shielding cloth 92 to pull it out or roll it back relative to the winding roller 93. When the hollow part of the light-shielding cloth 92 corresponds to the hollow groove on the movable oscillation seat 33, the lighting structure provides the light required for embryo development. When other parts of the light-shielding cloth 92 rotate to the position corresponding to the hollow groove of the movable oscillation seat 33, at this time, the position directly below the hole of the porous plate 7 is in a dark state, and the light generated by the lighting structure will overflow from the surrounding position of the light-shielding cloth 92. As a result, the light contrast between the lower part of the porous plate 7 and the environment increases, and then it is more convenient for the test operator to observe the development of the embryos in the porous plate 7. At the same time, it can also ensure the normal light conditions required for the embryos when not observing. The device is more flexible and has good practicability.

[0040] Embodiment 2

[0041] As Figure 2As shown, on the basis of Embodiment 1, in order to further save labor and ensure the stability of the movable oscillation seat 33 relative to the movable structure of the carrier 3. The carrier 3 includes a first gantry 31, a panel 32 provided on the first gantry 31, elastic telescopic members 34 provided at both ends of the movable oscillation seat 33 and movably inserted into the panel 32, and a driving oscillation unit 10 provided on the first gantry 31 for driving the movable oscillation seat 33 to oscillate. Among them, the driving oscillation unit 10 includes a transmission rod 101 pivotally connected to the first gantry 31 and an eccentric connecting member 103. The transmission rod 101 rotates synchronously with the eccentric connecting member 103 through a transmission member 102. When the eccentric connecting member 103 rotates, its outer end periodically and intermittently pushes the movable oscillation seat 33, realizing the oscillating swing of the movable oscillation seat 33. The oscillating swing of the movable oscillation seat 33 can, on the one hand, make the cyanotoxin working solution mix evenly with the embryos and culture solution in the porous plate 7 when the liquid injection unit 4 injects the cyanotoxin working solution into the porous plate 7. On the other hand, when the test operator regularly observes the embryonic development, it is convenient to change the position of the embryos growing adherently in the porous plate 7, facilitating statistics and recording.

[0042] In specific applications, the power source for driving the rotation of the transmission rod 101 is preferably installed on the door panel structure, preferably a driving motor. The transmission rod 101 is connected to the driving source of the driving motor and rotates under the drive of the driving motor, driving the eccentric connecting member 103 to rotate through the transmission member 102. The transmission member 102 is preferably a meshing gear set. The eccentric connecting member 103 is preferably a cam member. When it rotates, its path is periodically cyclic, so that its outer end can periodically and intermittently push the movable oscillation seat 33 to generate a displacement relative to the panel 32. The magnitude of the displacement of the movable oscillation seat 33 is related to the maximum diameter value of the cam member.

[0043] Embodiment 3

[0044] On the basis of Embodiment 1, in order to facilitate the test operator to use the observation unit 6, as Figure 3 shown, the structure of the observation unit 6 includes a cylinder 62 connected to the multi-directional adjustment structure 8, an observation eyepiece 61 provided at one end of the cylinder 62 outside the incubation chamber 5, and a convex lens 63 provided at one end of the cylinder 62 inside the incubation chamber 5. The convex lens 63 preferably uses a combination of an objective lens and an eyepiece, similar to the relevant structure of a microscope, aiming to magnify the morphology of the embryos in the porous plate 7.

[0045] In specific applications, the eyes of the experimental operator can be fitted with the position of the observation eye mask 61, and the multi-hole plate 7 on the carrier 3 in the incubation chamber 5 can be magnified through the convex lens 63 so that the morphology of the embryos in each hole of the multi-hole plate 7 can be clearly displayed. In order to facilitate the flexible adjustment of the observation unit 6 according to the usage requirements of the experimental operator, in this embodiment, a multi-directional adjustment structure 8 is further provided. The structure of the multi-directional adjustment structure 8 includes an elastic membrane 82 arranged at the top of the incubation chamber 5. The elastic membrane 82 is set to an opaque material to meet the requirements of dark embryo culture, and has good elasticity to meet the movement of the observation unit 6. A mounting seat 83 is arranged on the elastic membrane 82 to fix the observation unit 6, an annular slide rail 81 arranged in the incubation chamber 5, a sliding block 86 movably arranged in the annular slide rail 81, a telescopic rod 85 with one end hinged to the outside of the sliding block 86, and an articulated seat 84 hinged to the other end of the telescopic rod 85, wherein the articulated seat 84 is hinged to one end of the observation unit 6 extending into the incubation chamber 5, and the telescopic rod 85 is preferably two, and the sliding block 86 is preferably a spherical slider. The track shape of the annular slide rail 81 matches it. When the observation unit 6 is not in use, the telescopic rod 85 and its two The cooperation between the sliding block 86 and the hinged seat 84 at the end can realize the structural support of the observation unit 6 and avoid the elastic membrane 82 from being subjected to stress, thereby ensuring the service life of the elastic membrane 82. The telescopic rod 85 can also meet the support requirements of the observation unit 6 when it moves. Specifically, when the observation unit 6 needs to move horizontally, the displacement requirement of the observation unit 6 can be achieved through the extension and contraction of the telescopic rod 85 and the sliding block 86 connected to the telescopic rod 85 sliding on the annular slide rail 81. When the observation unit 6 needs to move vertically, it can be achieved through the rotation of one end of the telescopic rod 85 and the sliding block 86, and the other end and the observation unit 6. It has high operational flexibility. Compared with a multi-axial manipulator, the multi-directional adjustment structure 8 provided in this embodiment has a simple structure, a small footprint, low use and maintenance costs, and a good use effect.

[0046] Example 4

[0047] Based on Example 1, this example provides a new structure of a liquid injection unit 4, such as Figure 1 , 5As shown in the figure, the structure of the liquid injection unit 4 includes a second gantry 41. At the top of the second gantry 41, a number of pumping and liquid injection cylinders 42 are provided. A liquid injection head 43 is connected to the pumping and liquid injection cylinder 42 through a delivery pipe. A cross plate 45 is connected to the liquid injection head 43. A positioning rod 44 is movably sleeved at the lower end of the second gantry 41 and the cross plate 45. An electric guide rail mechanism two 47 is arranged in pairs in the length direction of the second gantry 41. A horizontal shaft 48 is arranged on the front side of the delivery pipe and is driven to move by the electric guide rail mechanism two 47. The number of pumping and liquid injection cylinders 42 is set according to the concentration gradient required for the cyanotoxin working solution during the test. Different high-concentration cyanotoxin working solutions are stored in each pumping and liquid injection cylinder 42. A travel switch 46 electrically connected to the electric guide rail mechanism two 47 is provided at the bottom end of the positioning rod 44. At the position corresponding to the positioning rod 44 on the carrier 3, a convex platform 13 with a height equal to the placement height of the porous plate 7 is provided.

[0048] During specific application, when the electric guide rail mechanism one 2 drives the carrier 3 to move to the liquid injection unit 4, the convex platform 13 on the carrier 3 contacts the travel switch 46 at the lower end of the positioning rod 44. After the travel switch 46 acts, the electric guide rail mechanism two 47 and the pumping and liquid injection cylinder 42 are started. The liquid injection volume of the pumping and liquid injection cylinder 42 is set through the control device of the device. The distance that the electric guide rail mechanism two 47 controls the horizontal shaft 48 to reciprocate is designed according to the reciprocating height of the liquid injection head 43 and the distance between adjacent holes of the porous plate 7 and is set through the control device of the device at the same time. During the process that the electric guide rail mechanism two 47 controls the horizontal shaft 48 to reciprocate, the horizontal shaft 48 contacts the delivery pipe and drives the delivery pipe to pull the connected liquid injection head 43 and the cross plate 45 to realize lifting displacement under the limitation of the positioning rod 44. At the same time, in cooperation with the work of the pumping and liquid injection cylinder 42, a continuous liquid injection operation is realized. The equipment has good mobility. When the carrier 3 moves until the convex platform 13 does not contact the lower end of the positioning rod 44, the electric guide rail mechanism two 47 and the pumping and liquid injection cylinder 42 are immediately closed. The equipment has good mobility and can synchronously realize the operation of injecting different high-concentration cyanotoxin working solutions into the corresponding porous plates 7. Compared with the manual addition method, it saves more time and improves efficiency.

[0049] Example 5

[0050] On the basis of Example 1, this embodiment further provides an evaluation method for the developmental toxicity of high-concentration cyanotoxin based on fish embryos using the evaluation device as described in any one of the above, including the following steps:

[0051] Step 1: Select a number of normally developed zebrafish embryos, place them in equal numbers in the holes of a number of porous plates 7 respectively, and then place the number of porous plates 7 in the movable oscillation seat 33 on the carrier 3.

[0052] Step 2: Drive the porous plate 7 on the carrier 3 to move to the liquid injection unit 4 through the electric guide rail mechanism 1. Inject different concentrations of cyanotoxin working solutions with equal volumes into several porous plates 7 respectively through the liquid injection unit 4, and control the movable oscillation base 33 to oscillate to mix the zebrafish embryos and the cyanotoxin working solutions evenly;

[0053] Step 3: Drive the porous plate 7 on the carrier 3 to move into the hatching room 5 through the electric guide rail mechanism 1, and provide light conditions through the lighting structure to hatch the zebrafish embryos in the hatching room 5 under the set hatching time;

[0054] Step 4: During the hatching process, observe the growth state of the zebrafish embryos through the observation unit 6, regularly record the number of dead, hatched, and deformed zebrafish embryos, and use the mortality rate, hatching rate, and malformation incidence rate of the zebrafish embryos as evaluation indicators to evaluate the developmental toxicity of cyanotoxin to the zebrafish embryos.

[0055] The above embodiments only represent several implementation manners of the present invention, and their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of the present invention patent. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several deformations and improvements can still be made, and these all belong to the protection scope of the present invention.

Claims

1. A device for evaluating the developmental toxicity of high-concentration cyanobacterial toxins based on fish embryos, comprising a base (1), characterized in that: It also includes an electric guide rail mechanism (2) extending along the length direction of the base (1) into the incubation chamber (5); A carrier (3) is slidably arranged on an electric guide rail mechanism (2), the carrier (3) is provided with a movable oscillating seat (33), and the movable oscillating seat (33) is provided with a plurality of porous plates (7) for placing fish embryos, the movable oscillating seat (33) is provided with hollow grooves at positions corresponding to the holes of the porous plates (7), and the carrier (3) is provided with an illumination structure at a position below the movable oscillating seat (33) for providing the required illumination conditions for the fish embryos; A liquid injection unit (4) and an incubation chamber (5) are arranged extending along the length direction of the machine base (1), wherein the liquid injection unit (4) is used to inject cyanobacteria toxin working solutions of different high concentrations into a plurality of porous plates (7), and an observation unit (6) is arranged on the top of the incubation chamber (5); And an adjustable shading unit (9) is arranged between the lighting structure and the porous plate (7), and is used to adjust the contrast between the bottom of the porous plate (7) and the environment, so as to facilitate observation of the development of fish embryos through the observation unit (6). The structure of the adjustable shading unit (9) includes an active roller (91), a winding roller (93), and a shading cloth (92) arranged between the active roller (91) and the winding roller (93), and the shading cloth (92) has a hollow portion.

2. The high-concentration cyanobacterial toxin developmental toxicity evaluation device based on fish embryos according to claim 1, characterized in that: The object carrier (3) comprises a gantry frame (31), a panel (32) arranged on the gantry frame (31), elastic telescopic parts (34) arranged at both ends of a movable oscillation seat (33) and movably connected to the panel (32), and a driving oscillation unit (10) arranged on the gantry frame (31) and used for driving the movable oscillation seat to oscillate.

3. The high-concentration cyanobacterial toxin developmental toxicity evaluation device based on fish embryos according to claim 2 is characterized by: The driving oscillation unit (10) comprises a transmission rod (101) and an eccentric connecting member (103) axially connected to a gantry frame (31); the transmission rod (101) rotates synchronously with the eccentric connecting member (103) via a transmission member (102); when the eccentric connecting member (103) rotates, the outer end of the eccentric connecting member (103) intermittently and periodically pushes the movable oscillation seat (33) to achieve oscillation and swing of the movable oscillation seat (33).

4. The high-concentration cyanobacterial toxin developmental toxicity evaluation device based on fish embryos according to claim 1, characterized in that: The top of the incubation chamber (5) is provided with a multi-directional adjustment structure (8) mounted on the observation unit (6), the structure of the multi-directional adjustment structure (8) comprising an elastic membrane (82) arranged at the top of the incubation chamber (5), a mounting seat (83) arranged on the elastic membrane (82) to fix the observation unit (6), an annular slide rail (81) arranged in the incubation chamber (5), a sliding block (86) movably arranged in the annular slide rail (81), a telescopic rod (85) with one end hinged to the outer side of the sliding block (86), and an articulated seat (84) hinged to the other end of the telescopic rod (85), the articulated seat (84) being articulated to one end of the observation unit (6) extending into the incubation chamber (5).

5. The high-concentration cyanobacterial toxin developmental toxicity evaluation device based on fish embryos according to claim 1, characterized in that: The structure of the observation unit (6) comprises a cylinder (62) connected to the multi-directional adjustment structure (8), an observation eye mask (61) arranged at one end of the cylinder (62) located outside the incubation chamber (5), and a convex lens (63) arranged at one end of the cylinder (62) located inside the incubation chamber (5).

6. The high-concentration cyanobacterial toxin developmental toxicity evaluation device based on fish embryos according to claim 1, characterized in that: The structure of the liquid injection unit (4) comprises a gantry frame (41), a plurality of pumping liquid injection cylinders (42) arranged at the top of the gantry frame (41), a liquid injection head (43) connected to the pumping liquid injection cylinder (42) through a delivery pipe, a horizontal plate (45) connected to the liquid injection head (43), a positioning rod (44) arranged at the lower end of the gantry frame (41) and movably sleeved with the horizontal plate (45), two electric guide rail mechanisms (47) arranged in pairs in the length direction of the gantry frame (41), and a horizontal shaft (48) arranged at the front side of the delivery pipe and driven to move by the electric guide rail mechanism (47), a travel switch (46) electrically connected to the electric guide rail mechanism (47) is arranged at the bottom end of the positioning rod (44), and a boss (13) whose height is equal to the placement height of the porous plate (7) is arranged at a position corresponding to the positioning rod (44) on the carrier (3).

7. The high-concentration cyanobacterial toxin developmental toxicity evaluation device based on fish embryos according to claim 1, characterized in that: An operating glove (11) is installed on the side of the incubation chamber (5) opposite to the liquid injection unit (4), and a tool box (12) is slidably arranged in the incubation chamber (5).

8. A method for evaluating the developmental toxicity of high-concentration cyanobacterial toxins based on fish embryos using the device according to any one of claims 1 to 7, characterized in that: The following steps are involved: Step 1: Select a number of zebrafish embryos with normal development, place an equal number of them in the holes of a number of porous plates (7), and then place the number of porous plates (7) in a movable oscillating seat (33) on the carrier (3); Step 2: The multi-hole plate (7) on the carrier (3) is driven to move to the liquid injection unit (4) by the electric guide rail mechanism 1 (2), and the same volume of cyanobacteria toxin working solution of different concentrations is respectively injected into a plurality of multi-hole plates (7) by the liquid injection unit (4), and the zebrafish embryos and the cyanobacteria toxin working solution are uniformly mixed by controlling the movable oscillating seat (33) to oscillate; Step 3: driving the multi-hole plate (7) on the carrier (3) to move into the incubation chamber (5) through the electric guide rail mechanism 1 (2), and providing lighting conditions through the lighting structure to incubate the zebrafish embryos in the incubation chamber (5) at a set incubation time; Step 4: During the incubation process, the growth status of the zebrafish embryos is observed by an observation unit (6), and the number of deaths, hatchings and deformities of the zebrafish embryos are regularly recorded. The mortality rate, hatching rate and deformity incidence rate of the zebrafish embryos are used as evaluation indicators to evaluate the developmental toxicity of cyanobacterial toxins on the zebrafish embryos.

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