Application of agrobacterium tumefaciens Y2 in promoting soil nitrification and plant growth
By screening and isolating the obtained Agrobacterium tumefaciens Y2 and inoculating the strain in the soil, the problem of lack of effective microbial resources in the prior art to regulate soil nitrogen transformation and promote plant growth is solved, and significant soil nitration and Plant growth and development effect.
Patent Information
- Application Number
- CN202411761476.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-03
- Publication Date
- 2025-02-18
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
No research reports on Agrobacterium tumefaciens in promoting plant growth and promoting soil nitration have been seen in the prior art, resulting in a lack of effective microbial resources in agricultural production to regulate soil nitrogen conversion and promote plant growth.
Agrobacterium tumefaciens Y2 obtained through screening and isolation, classified as Agrobacterium tumefaciens strain, has been deposited in the Chinese Type Culture Collection Center, with the collection number CCTCC M 20241833, and the strain is inoculated in the soil to promote soil nitration and Plant growth and development.
Agrobacterium tumefaciens Y2 significantly promotes soil nitration and plant growth and development, provides a new microbial resource, suitable for promotion and use in areas such as corn planting, and has the advantages of low production costs and easy use.
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Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of microorganisms and their applications, and relates to Agrobacterium tumefaciens bacteria for promoting soil nitrification and plant growth and development and applications thereof. Background Art
[0002] Soil nitrification is one of the important nitrogen transformation processes in the soil, which can convert ammonium nitrogen into nitrate nitrogen, and the produced nitrate nitrogen is an important source of soil nitrogen. Nitrogen, as an essential macronutrient for plants, is the main factor affecting plant growth and yield. Therefore, regulating soil nitrification by appropriate means to promote crop growth and development is of great significance to agricultural production.
[0003] Agrobacterium tumefaciens is widely present in natural environments such as soil and river sediments. Currently, there are many reports on the research of Agrobacterium tumefaciens in the field of environmental protection, but there are no research reports on its promotion of plant growth and soil nitrification in the prior art.
[0004] Invention Technology The technical objectives of the present invention are: 1. To provide the application of Agrobacterium tumefaciens Y2 in promoting soil nitrification; 2. To provide a method for promoting soil nitrification; 3. To provide the application of Agrobacterium tumefaciens Y2 in promoting plant growth and development; 4. To provide a method for promoting plant growth and development; The present invention explores the function of Agrobacterium tumefaciens Y2 screened from the soil and finds that it has the effects of promoting plant growth and development and promoting soil nitrification, thereby providing a new technical path for agricultural production.
[0005] In order to achieve the above object, the specific scheme adopted by the present invention is: application of Agrobacterium tumefaciens Y2 in promoting soil nitrification, wherein the Agrobacterium tumefaciens Y2 is classified and named Agrobacterium tumefaciens strain , and was deposited in the China Center for Type Culture Collection on August 21, 2024, with the deposit number CCTCC M 20241833.
[0006] A method for promoting soil nitrification, inoculating Agrobacterium tumefaciens Y2 in the soil; the Agrobacterium tumefaciens Y2 is classified and named Agrobacterium tumefaciens strain , and was deposited in the China Center for Type Culture Collection on August 21, 2024, with the deposit number CCTCC M 20241833.
[0007] Application of Agrobacterium tumefaciens Y2 in promoting plant growth and development, wherein the Agrobacterium tumefaciens Y2 is classified and named Agrobacterium tumefaciens strain , and was deposited in the China Center for Type Culture Collection on August 21, 2024, with the deposit number CCTCC M 20241833.
[0008] Furthermore, the plant is corn.
[0009] A method for promoting plant growth and development, inoculating Agrobacterium tumefaciens Y2 in soil; the Agrobacterium tumefaciens Y2 is classified and named Agrobacterium tumefaciens strain , and was deposited in the China Center for Type Culture Collection on August 21, 2024, with the deposit number CCTCC M 20241833.
[0010] Furthermore, before sowing the plants, the plant seeds are evenly mixed with Agrobacterium tumefaciens Y2 and then planted in the soil.
[0011] Beneficial effects: The present invention aims to promote plant growth, screens and separates Agrobacterium tumefaciens Y2 from the soil, and identifies and analyzes the growth and development of plants before and after seed dressing and the promotion of soil nitrification. It is found that Agrobacterium tumefaciens Y2 has significant effects in promoting plant growth and development and promoting soil nitrification. It can be applied to crop growth, providing a new microbial resource for agricultural production. In addition, the microbial agent prepared by using the Agrobacterium tumefaciens Y2 has the advantages of low production cost and convenient use, and is suitable for promotion and use in corn-growing areas across the country.
[0012] Bacterium deposit information: The Agrobacterium tumefaciens Y2 described in the present invention is classified and named Agrobacterium tumefaciens strain , has been deposited in the China Center for Type Culture Collection, the preservation date is August 21, 2024, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC: M20241833. BRIEF DESCRIPTION OF THE DRAWINGS
[0013] Figure 1 This is a colony morphology diagram of Agrobacterium tumefaciens Y2 in an isolated culture medium that has the function of promoting soil nitrification and promoting plant growth and development according to the present invention; Figure 2 It is an optical microscope picture of the colony morphology of Agrobacterium tumefaciens Y2 which has the function of promoting soil nitrification and promoting plant growth and development according to the present invention. DETAILED DESCRIPTION
[0014] The technical solution of the present invention is described more clearly and completely below in conjunction with the accompanying drawings and embodiments.
[0015] The experimental methods described in the following examples are conventional methods unless otherwise specified; the reagents and materials described are commercially available unless otherwise specified.
[0016] The inoculation amounts described in the following examples are all inoculated according to the corresponding volume ratios.
[0017] Example 1: Isolation and screening of Agrobacterium tumefaciens Y2 that promotes soil nitrification and plant growth and development 20g of soil from the experimental farm of Henan University of Science and Technology was taken and added to the enrichment medium, the formula of which was: (NH4)2SO40.5g, KH2PO40.75g, NaH2PO40.25g, MnSO4∙4H2O 0.01g, MgSO4∙7H2O 0.03g, CaCO35.0g, deionized water was added to 1000ml, pH value was 7.0-7.2, sterilized at 121℃ for 20 min, and cultured at 28℃ in the dark for 15-25 days. Then, Griess reagent was used to detect the presence of ammonia oxidizing bacterial strains, and the enrichment culture solution showing the growth of ammonia oxidizing bacterial strains was transferred to a new enrichment medium for continued culture. The process was repeated twice to obtain a pure bacterial enrichment solution.
[0018] Take 0.1ml of the enriched culture fluid obtained above, spread it on the agar plate separation medium, and culture it at 28℃ and dark conditions for 7 days. The formula of the agar plate separation medium is: (NH4)2SO40.5g, KH2PO40.75g, NaH2PO40.25g, MnSO4∙4H2O 0.01g, MgSO4∙7H2O 0.03 g, CaCO35.0 g, 2.5g agar, deionized water to 1000ml, pH 7.0~7.2, sterilize at 121℃ for 20 minutes. After 7 days, select a single colony from the above separation medium and spread it on the agar plate separation medium. Repeat this process 5 to 10 times to obtain a plate medium containing pure bacterial colonies.
[0019] On the plate culture medium containing pure bacterial colonies obtained above, 5 to 10 pure colonies were transplanted into an enrichment culture medium (formula as above), and cultured at 28°C in the dark for 15 days to obtain the Agrobacterium tumefaciens Y2 microbial agent described in this study.
[0020] The above Agrobacterium tumefaciens Y2, which has the function of promoting soil nitrification and promoting plant growth and development, is named Y2, and its classification is named Agrobacterium tumefaciens strain , and was deposited in the China Center for Type Culture Collection on August 21, 2024, with the deposit number CCTCC M 20241833.
[0021] Example 2: Determination of the effects of Agrobacterium tumefaciens Y2 on promoting soil nitrification and plant growth and development Take 12 pots, each pot is designed to be planted with 3 corns, and the pots are filled with soil. It is determined that each kilogram of soil in the pot contains 25.7g of organic matter. The 12 pots of corn are randomly divided into 3 groups, each with 4 pots, namely test groups a, b, and c, and the 4 pots in each group represent 4 repetitions.
[0022] 200 ml of bacterial solution was added to 1 kg of corn seeds for seed dressing. Test group a was used as a blank control, and corn seeds were directly sown. In test group b, corn seeds were mixed with bacterial solution in each pot when sowing. In test group c, the same proportion of pure enrichment solution was added to the seeds in each pot when sowing, stirred, and then sown as a control; that is, test group b was corn seeds mixed with Agrobacterium tumefaciens Y2 bacterial agent, and test group c was corn seeds mixed with enrichment solution.
[0023] Take out the roots, stems and leaves of corn seedlings grown 20 days after sowing, test group a; test group b; and test group c. Put these roots, stems and leaves into a drying box at a temperature of 75°C, and after drying, obtain the whole corn plant dried products, and then use an electronic balance to detect the weight of the dried products to obtain the dried product mass Ra of test group a, the dried product mass Rb of test group b, and the dried product mass Rc of test group c, and set them aside.
[0024] The soil nitrification rate of the rhizosphere soil of a, b, and c was determined by the culture method, and the soil nitrification rate Za after inoculation of the test group a was 0.047, the soil nitrification rate Zb after inoculation of the test group b was 0.182, and the soil nitrification rate Zc after inoculation of the test group c was 0.072, which were set aside for later use.
[0025] The test results show that Ra, Rb, and Rc are 2.21g, 3.34g, and 2.54g / pot, respectively. Comparing the sizes of Ra, Rb, and Rc, Rb>Rc>Ra, and Zb>Zc>Za, indicating that inoculation with Agrobacterium tumefaciens Y2 can effectively accelerate soil nitrification and promote the growth and development of corn. That is, inoculation with Agrobacterium tumefaciens Y2 can accelerate the growth and development of corn by promoting nitrification in the rhizosphere soil.
[0026] It should be noted that the above-described embodiments should be understood as illustrative rather than limiting the scope of protection of the present invention, and the scope of protection of the present invention shall be subject to the claims. For those skilled in the art, some non-essential improvements and adjustments made to the present invention still fall within the scope of protection of the present invention without departing from the essence and scope of the present invention.
Claims
1. Application of Agrobacterium tumefaciens Y2 in promoting soil nitrification, characterized in that: The Agrobacterium tumefaciens Y2 is classified as Agrobacterium tumefaciens strain , and was deposited in the China Center for Type Culture Collection on August 21, 2024, with the deposit number CCTCC M 20241833.
2. A method for promoting soil nitrification, characterized in that: Inoculate Agrobacterium tumefaciens Y2 in soil; the Agrobacterium tumefaciens Y2 is classified and named Agrobacterium tumefaciens strain , and was deposited in the China Center for Type Culture Collection on August 21, 2024, with the deposit number CCTCC M 20241833.
3. Application of Agrobacterium tumefaciens Y2 in promoting plant growth and development, characterized in that: The Agrobacterium tumefaciens Y2 is classified as Agrobacterium tumefaciens strain , and was deposited in the China Center for Type Culture Collection on August 21, 2024, with the deposit number CCTCC M 20241833.
4. The use of Agrobacterium tumefaciens Y2 in promoting plant growth and development according to claim 3, characterized in that: The plant is corn.
5. A method for promoting plant growth and development, characterized in that: Inoculate Agrobacterium tumefaciens Y2 in soil; the Agrobacterium tumefaciens Y2 is classified and named Agrobacterium tumefaciens strain , and was deposited in the China Center for Type Culture Collection on August 21, 2024, with the deposit number CCTCC M 20241833.
6. A method for promoting plant growth and development according to claim 5, characterized in that: Before sowing the plants, the plant seeds are mixed evenly with Agrobacterium tumefaciens Y2 and then planted in the soil.