Clostridium ethanolicum enzymolysis product, new application of clostridium ethanolicum enzymolysis product and cat strip

Abstract

The invention belongs to the field of pet food, and discloses a clostridium ethanolicum enzymolysis product which is obtained by performing enzymolysis on clostridium ethanolicum. Compared with the clostridium ethanol protein, the clostridium ethanol protein can obviously improve the hair color brightness of cats. Meanwhile, the enzymolysis product can assist in controlling the weight of the cats and improving the health of the cats. The invention also discloses a new application of the clostridium ethanolicum enzymolysis product, and a cat strip.

Description

Technical Field

[0001] The invention relates to the field of pet food, in particular to an alcoholic clostridium enzymolysis product, a new use of the alcoholic clostridium enzymolysis product, and a cat strip. Background Art

[0002] Cat bars, also known as cat snack bars or nutritional bars, are a type of food specially designed for cats, usually with rich nutrients and specific functionality. Through continuous technological updates and scientific research, cat food bars not only meet the taste and nutritional needs of cats, but also provide pet owners with more choices and convenience. With the development of the market, more innovative products may emerge in the future.

[0003] In the patent application with Chinese patent publication number CN112056466A, the subject is the application of ethanolic clostridium protein in pet feed, which discloses the application of ethanolic clostridium protein to replace plant protein and reduce anti-nutritional factors; the scheme points out the direct application method of ethanolic clostridium protein in pet feed.

[0004] In the process of using ethanolic Clostridium protein to prepare cat strips, the present application discovered that the deep-processed products of ethanolic Clostridium protein have new application prospects.

[0005] The problem that this program needs to solve: How to improve the health and coat color of cats. Summary of the invention

[0006] The purpose of the present invention is to provide an alcoholic clostridium enzymolysis product and its application in controlling the fur color of cats, which can significantly improve the fur color brightness of cats compared with alcoholic clostridium protein. At the same time, the enzymolysis product can also help control the weight of cats and improve the health of cats.

[0007] In addition, the invention also provides a cat strip.

[0008] The invention discloses an alcoholic clostridium enzymolysis product, which is obtained by enzymolysis with alcoholic clostridium.

[0009] In the above-mentioned enzymatic hydrolysis product of Clostridium ethanolum, the enzymatic hydrolysis product of Clostridium ethanolum is a product obtained by performing a primary enzymatic hydrolysis with β-glucanase and a secondary enzymatic hydrolysis with one or both of neutral protease and papain.

[0010] In the above-mentioned alcoholic Clostridium enzymatic hydrolysate, the mass ratio of the β-glucanase, neutral protease and papain is 1-2: 0.1-0.3: 0.1-0.2

[0011] To achieve the above objectives, the present application discloses the use of the enzymatic hydrolysate of Clostridium ethanolum as a cat feed additive.

[0012] The enzymatic hydrolysate of ethanolic Clostridium can improve the fur color of cats. The principle is that the ethanolic Clostridium protein is decomposed into tyrosine, phenylalanine and other flavor amino acids through enzymatic decomposition, which is beneficial to the deposition of melanin in cat hair and beautifies cat hair. In the patent application with Chinese patent publication number CN112056466A, the subject of which is the application of ethanolic Clostridium protein in pet feed, the application of ethanolic Clostridium protein to replace plant protein and reduce anti-nutritional factors is disclosed. The scheme points out the direct application method of ethanolic Clostridium protein in pet feed. The special contribution of the present invention is that it is surprisingly found that after using the ethanolic Clostridium enzymatic hydrolysate, it is more beneficial to the growth and health of animals, is beneficial to the absorption and utilization of nutrients by cats, is beneficial to the deposition of melanin in cat hair and beautifies cat hair.

[0013] In the above-mentioned use, the cat feed additive is an additive for improving the coat color of cats.

[0014] In the above-mentioned use, the enzymatic hydrolysis product of Clostridium ethanolum is a product obtained by a primary enzymatic hydrolysis with β-glucanase and a secondary enzymatic hydrolysis with one or both of neutral protease and papain.

[0015] In the above-mentioned use, the mass ratio of the β-glucanase, the neutral protease and the papain is 1-2:0.1-0.3:0.1-0.2.

[0016] At the same time, the present invention also discloses a cat strip, comprising any of the above-mentioned clostridium ethanolic hydrolysate, fermented plant fiber, and fatty acid mixture, wherein the mass ratio of the clostridium ethanolic hydrolysate, fermented plant fiber, and fatty acid mixture is 2-4:15-20:4-8; the fatty acid mixture comprises wall material and fatty acids wrapped in the wall material.

[0017] In the technical solution of the present invention, a fatty acid mixture wrapped with resistant dextrin, carboxymethyl chitosan and sodium alginate as wall materials is added, which can protect the oral nutritional supplement from being destroyed by gastric acid and digestive enzymes while ensuring release and absorption in the intestine;

[0018] The ethanolic clostridium enzymolysis liquid is added, and the ethanolic clostridium protein is decomposed into tyrosine, phenylalanine and other flavor amino acids through enzyme decomposition, which is beneficial to the deposition of melanin in cat hair and beautifies cat hair; in the patent application with the theme of application of ethanolic clostridium protein in pet feed with Chinese patent publication number CN112056466A, the application of ethanolic clostridium protein to replace plant protein and reduce anti-nutritional factors is disclosed; the scheme points out the direct application mode of ethanolic clostridium protein in pet feed, and the special contribution of the present invention is that it is surprisingly found that after using the ethanolic clostridium enzymolysis liquid, it is more beneficial to the growth and health of animals, is beneficial to the absorption and utilization of nutrients by cats, is beneficial to the deposition of melanin in cat hair, and beautifies cat hair;

[0019] The addition of fermented plant fiber not only provides energy for colon cells through the acetic acid, propionic acid and butyric acid produced by it, but also enters the liver through the portal vein circulation, where it is used as an energy substrate and participates in the biosynthesis of glucose, cholesterol and fatty acids. The plant fiber also contains bamboo shoot fiber and konjac flour. Bamboo shoot fiber prevents obesity by regulating intestinal flora, and konjac flour can promote intestinal peristalsis, help defecation and reduce the residence time of harmful substances in the intestine. In addition, other functional substances are added to provide other nutrients for the health of cats.

[0020] By using the above three main nutrients, you can effectively regulate the cat's weight and solve the problem of obesity, increase daily water intake, and benefit the health of hair and feces.

[0021] In the above cat strips, the fermented plant fiber is obtained by fermenting plant fiber with microorganisms, and the mass ratio of plant fiber to microorganism is 96-99:1-4;

[0022] The microorganism is selected from at least one of Lactobacillus plantarum, Lactobacillus bulgaricus and Lactobacillus acidophilus; the plant fiber is oat fiber, bamboo shoot fiber and konjac fiber, and the mass ratio of oat fiber, bamboo shoot fiber and konjac fiber is 20-50:5-20:5-10.

[0023] In the above cat strips, the plant fiber also includes psyllium fiber, carrot fiber, and broccoli fiber;

[0024] The plant fiber is composed of the following components by mass fraction:

[0025] 20-50% oat fiber;

[0026] 5-20% bamboo shoot fiber;

[0027] 5-10% konjac fiber;

[0028] 0-5% psyllium fiber;

[0029] 2-5% carrot fiber;

[0030] 2-5% broccoli fiber;

[0031] The plant fiber powder is supplemented to a total mass fraction of 100%.

[0032] In the above cat strips, the wall material is prepared by mixing resistant dextrin, carboxymethyl chitosan and sodium alginate; the fatty acid is a medium-chain fatty acid, and the fatty acid also contains fish oil, soybean lecithin and vitamin E.

[0033] In the above cat strips, it also includes complex beneficial ingredients and / or functional substances;

[0034] The mass ratio of the composite beneficial ingredients to the enzymatic hydrolysate of ethanolic Clostridium is 2-5:2-4;

[0035] The composite beneficial component is a mixture of taurine, L-carnitine, Poria cocos extract, and resveratrol, and the mass ratio of taurine, L-carnitine, Poria cocos extract, and resveratrol in the mixture is 30-50:10-20:10-20:5-10;

[0036] The effects of compound beneficial ingredients mainly include the following aspects:

[0037] Promote intestinal motility: Taurine and L-carnitine help promote intestinal motility, aid in food digestion and absorption, and reduce the occurrence of constipation.

[0038] Enhance intestinal barrier function: Poria cocos extract has the effect of enhancing intestinal barrier function, which can reduce the intestinal absorption of harmful substances and protect intestinal health.

[0039] Antioxidant effect: Resveratrol has a strong antioxidant effect, which can reduce free radical damage in the intestines and protect intestinal cells from oxidative stress.

[0040] Regulate intestinal flora: The ingredients in the compound prebiotics can regulate intestinal flora, promote the growth of beneficial bacteria, inhibit the reproduction of harmful bacteria, and maintain the balance of intestinal microecology.

[0041] Enhance immunity: Intestinal health is closely related to the immune system. Compound prebiotic ingredients can enhance the body's immune function by improving intestinal health.

[0042] The mass ratio of the functional substance to the enzymatic hydrolysate of ethanolic Clostridium is 2:2-4;

[0043] The functional substance is composed of complex vitamins and minerals, and the mass ratio of the two is 0.1-0.5:0.2-0.5;

[0044] The compound vitamins are vitamin A, vitamin D, vitamin E and vitamin K;

[0045] The minerals are copper sulfate, potassium iodide, ferrous sulfate, manganese sulfate, sodium selenite and zinc sulfate.

[0046] In the above cat strips, the following components are composed by mass percentage:

[0047] 40%-60% chicken;

[0048] 5% to 10% krill powder;

[0049] 2% to 4% ethanolic Clostridium enzymatic hydrolysate;

[0050] 2%-4% chicken liver;

[0051] 15-20% fermented plant fiber;

[0052] 4-8% fatty acid mixture;

[0053] 2-5% compound beneficial ingredients;

[0054] 2% functional substances;

[0055] Add water to a total mass fraction of 100%

[0056] The beneficial effects of the present invention are:

[0057] 1. The present invention has experimentally verified the contribution of the enzymatic hydrolysate of Clostridium ethanolum to the improvement of the cat's coat color;

[0058] 2. The invention provides a cat strip, which is added with a fatty acid mixture wrapped with resistant dextrin, carboxymethyl chitosan and sodium alginate as wall materials, can protect the oral nutritional supplement from being destroyed by gastric acid and digestive enzymes, and ensures release and absorption in the intestine; it is added with ethanolic clostridium enzymolysis liquid, and the ethanolic clostridium protein is decomposed into tyrosine and phenylalanine through enzyme decomposition, which is beneficial to the deposition of melanin in cat hair and beautifies cat hair; it is added with fermented plant fiber, and the acetic acid, propionic acid and butyric acid produced by the fermented plant fiber not only provide energy for colon cells, but also enter the liver through the portal vein circulation, where they are used as energy substrates and participate in the biosynthesis of glucose, cholesterol and fatty acids, and the plant fiber also contains bamboo shoot fiber and konjac flour, the bamboo shoot fiber prevents obesity by regulating intestinal flora, and the konjac flour can promote intestinal peristalsis, help defecation and reduce the residence time of harmful substances in the intestine; in addition, it is added with other functional substances to provide other nutrients for the health of cats. DETAILED DESCRIPTION

[0059] In the description of the present invention, it should be noted that, if the specific conditions are not specified in the examples, the experiments were carried out according to conventional conditions or the conditions recommended by the manufacturer. The reagents or instruments used, if the manufacturer is not specified, are all conventional products that can be purchased commercially.

[0060] Part I Raw Materials and Their Preparation

[0061] The raw material information of the product is shown in Table 1:

[0062] Table 1 Raw material sources

[0063]

[0064]

[0065] In the following examples and comparative examples, the preparation method of the fatty acid mixture is as follows:

[0066] Step 1: dissolving resistant dextrin, carboxymethyl chitosan and sodium alginate in water at a mass ratio of 6:3:1 at 70° C. to obtain a wall material solution;

[0067] Step 2: Weigh medium-chain fatty acids, fish oil, soybean lecithin, and vitamin E in a mass ratio of 25% and add them to the wall material solution, use a high-speed dispersing homogenizer to disperse and homogenize at 18000 r / min for 8 minutes, and homogenize at 50 MPa for 4 times to obtain a mixed emulsion;

[0068] Step 3: spray-dry the mixed emulsion, control the inlet temperature at 170-180° C., and the outlet temperature at 85-95° C. to obtain a fatty acid mixture wrapped with a wall material.

[0069] The preparation methods of fermented plant fibers are:

[0070] Step 1: fresh carrots and broccoli are crushed into a paste by a homogenizer, and added into the plant fiber powder together with oats, bamboo shoot fiber, konjac flour and psyllium, and stirred evenly, and distilled water is added to make the water content of the mixture 50%, and sterilized by high pressure steam at 121° C. for 15 minutes to obtain a sterilized solid component;

[0071] Step 2: Inoculate the fermentation bacteria onto MRS solid culture medium and culture in a 37°C incubator for 24 hours; then select a single colony and transfer it to MRS broth culture medium, and culture it in a 37°C incubator for another 24 hours to obtain the test strain, shake the MRS broth culture medium of the test strain, take 5 ml into a sterile centrifuge tube, centrifuge at 4500r / min for 15 minutes, skim off the supernatant, wash with 0.85% sterile saline, and centrifuge to obtain the lactic acid bacteria mother liquor.

[0072] Step 3: Add 1000 mL of 7 The bacterial suspension of CFU / mL fermentation bacteria and 100g dry yeast was mixed with 10kg sterilized solid components, loaded into a 50kg solid fermentation tank, and fermented at 37°C for 48h to obtain fermented plant fiber.

[0073] Part II Preparation of enzymatic hydrolysate of Clostridium ethanolum

[0074] The ethanolic Clostridium cells and water were mixed at a mass ratio of 4:6 to obtain an ethanolic Clostridium cell suspension; β-glucanase was then added, the solution pH was adjusted to 4.8, the temperature was 50°C, and the solution was hydrolyzed for 8 hours;

[0075] Neutral protease and papain were then added for enzymolysis, the pH of the solution was adjusted to 6.0, and the solution was hydrolyzed for 8 hours to obtain ethanolic Clostridium enzymolysis solution.

[0076] The types and dosages of the enzymes can be referred to Table 1. In Table 1, the dosage of the enzymes is based on the total amount of ethanol Clostridium as 100 wt %;

[0077] Table 1 Enzyme types and dosage

[0078] β-Glucanase Neutral protease Papain Ethanol Clostridium ethanolicum enzymatic hydrolysate 1 2wt% 0.3wt% 0.2wt% Ethanol Clostridium enzymolysis product 2 1wt% 0.3wt% 0.2wt% Ethanol Clostridium enzymolysis product 3 2wt% 0.1wt% 0.1wt% Ethanol Clostridium enzymolysis product 4 2wt% 0.5wt% 0 Ethanol Clostridium enzymolysis product 5 2wt% 0 0.5wt%

[0079] Examples 1-3

[0080] The ingredients of a cat strip are shown in Table 2:

[0081] Table 2

[0082] Example 1 Example 2 Example 3 chicken 50％ 50％ 50％ Krill Meal 7％ 7％ 7％ Chicken liver 3％ 3％ 3％ Ethanol Clostridium enzymatic hydrolysate 1 3％ 2％ 4％ Fermented plant fiber 17％ 15％ 20％ Fatty acid mixture 6％ 4％ 8％ Compound beneficial ingredients 3％ 2％ 5％ Functional substances 2％ 2％ 2％ water Add to 100% Add to 100% Add to 100%

[0083] The preparation method of cat strips is as follows:

[0084] Step 1: Mix chicken, krill meal, ethanolic clostridium enzymatic hydrolysate, fermented plant fiber, fatty acid mixture, compound probiotic ingredients, compound vitamins and minerals, and water to obtain a mixture;

[0085] Step 2: The mixture is sterilized at 180°C for 8 minutes, and then the temperature is reduced to 80°C for low-temperature baking, and the moisture content is controlled within 31% to obtain a finished product;

[0086] Step 3: Put the finished product into packaging bags, fill 10g in each bag, and pack it with a vacuum machine to obtain cat strips.

[0087] The plant fiber in the fermented plant fiber is composed of the following components in weight percentage: 35% oats, 12% bamboo shoot fiber, 7% konjac flour, 3% psyllium, 3% carrots, 3% broccoli, and the plant fiber powder is supplemented to 100%.

[0088] The mass ratio of plant fiber to plant lactobacillus in the fermented plant fiber is 99:1.

[0089] Example 4

[0090] The method is basically the same as Example 1, except that the enzymatic hydrolysate of ethanolic Clostridium is enzymatic hydrolysate 2 of ethanolic Clostridium.

[0091] Example 5

[0092] The method is basically the same as Example 1, except that the enzymatic hydrolysate of Clostridium ethanolicum used is the enzymatic hydrolysate 3 of Clostridium ethanolicum.

[0093] Example 6

[0094] The method is basically the same as Example 1, except that the enzymatic hydrolysate of ethanolic Clostridium is enzymatic hydrolysate 4.

[0095] Example 7

[0096] The method is basically the same as Example 1, except that the enzymatic hydrolysate of ethanolic Clostridium is ethanolic Clostridium enzymatic hydrolysate 5.

[0097] Comparative Example 1

[0098] The method is basically the same as Example 1, except that the ethanolic Clostridium enzymatic hydrolysate 1 is replaced by an equal amount of ethanolic Clostridium protein.

[0099] Comparative Example 2

[0100] The method is basically the same as Example 1, except that no ethanolic Clostridium enzymatic hydrolysate is added to the cat strips, and the amount of chicken is adjusted to 53 wt %.

[0101] Performance Test:

[0102] Test method: 95 healthy American shorthair cats with an average weight of 5.49±0.45 kg aged 12-24 months were provided, and 5 cats were randomly assigned to each group. The cats were individually housed in cat cages with a relative humidity and temperature of 70% and 22°C±2°C. During the experiment, the cats were free to eat enough fresh food and drink clean water. The pens were cleaned and disinfected regularly to keep the pens clean. The feeding conditions and mental states of all pets were observed during the trial period. The dry food group was fed commercial dry food, and the cat strip group was fed 10 grams of cat strips prepared in the above embodiments and comparative examples every day. The other conditions were consistent with the dry food group, and the experimental period was 90 days.

[0103] Feces scoring method: Through the color, shape, contents, etc. of the feces, a rough analysis of the pet's physical condition can be made. 5 points: extremely low water content, dry, fragile, and blocky. 4 points: There are clearly visible lines and cracks on the surface of the feces, which are easy to pick up and basically no residue on the ground. 3 points: The feces are moist and cylindrical, and there will be residue when picked up from the ground. 2 points: The feces are relatively wet, but not completely liquid. It is not easy to pick up, and sometimes it will stick to the pet's anus. 1 point: Completely liquid feces. In the feces scoring system, 3 points is the most ideal shape.

[0104] Hair quality test method: Five professionals conducted a double-blind evaluation of the sensory indicators of fur quality of each group of cats according to the fur condition evaluation criteria (Table 3).

[0105] Table 3 Fur quality evaluation standards

[0106]

[0107] The test results are shown in Table 4:

[0108] Table 3 Test results

[0109]

[0110]

[0111] Result analysis:

[0112] 1. By comparing the data of Examples 1-7, Comparative Examples 1 and 2, and the dry food group, the body weight and daily food intake of the Example group were reduced, the amount of water consumed was increased, and the feces score was increased, indicating that the use of ethanolic Clostridium enzymatic hydrolysate can improve the utilization rate of nutrients by cats; as can be seen from the coat color quality, the coat color of the experimental group of the embodiment of the present invention is significantly higher than that of the comparative example group and the dry food group, indicating that the ethanolic Clostridium enzymatic hydrolysate can promote the deposition of melanin while improving the absorption of nutrients, thereby improving the coat color effect.

[0113] 2. According to the results of Example 1 to Example 3, when the ratio of ethanolic clostridium enzymolysis product, fermented plant fiber, fatty acid mixture, the ethanolic clostridium enzymolysis product, fermented plant fiber, fatty acid mixture is large, its effect is more obvious. According to the results of Example 4 to Example 7, adopting ethanolic clostridium enzymolysis product 1 is a relatively better choice, indicating that the combination and dosage of the enzyme used for enzymolysis are relatively important.

[0114] 3. The full effect of the present invention is not solely determined by the ethanolic Clostridium enzymolysis solution, but by the ethanolic Clostridium enzymolysis product, fermented plant fiber, and fatty acid mixture, which work together to produce the corresponding effect.

[0115] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principles of the present invention shall be equivalent replacement methods and shall be included in the protection scope of the present invention.

Claims

1. An enzymatic hydrolysate of Clostridium ethanolum, characterized in that: Obtained by enzymatic hydrolysis using Clostridium ethanolum.

2. The enzymatic hydrolysate of Clostridium ethanolum according to claim 1, characterized in that The ethanolic Clostridium enzymatic hydrolysis product is a product obtained by performing a primary enzymatic hydrolysis with β-glucanase and a secondary enzymatic hydrolysis with one or both of neutral protease and papain.

3. The enzymatic hydrolysate of Clostridium ethanolum according to claim 1, characterized in that The mass ratio of the beta-glucanase, neutral protease and papain is 1-2: 0.1-0.3: 0.1-0.

2.

4. Use of the enzymatic hydrolysate of Clostridium ethanolum as any one of claims 1 to 3 as a cat feed additive.

5. The use according to claim 4, characterized in that The cat feed additive is an additive used to improve the coat color of cats.

6. A cat strip, characterized in that: The method comprises the enzymatic hydrolysis product of ethanolic clostridium as claimed in any one of claims 1 to 3, fermented plant fiber and fatty acid mixture, wherein the mass ratio of the enzymatic hydrolysis product of ethanolic clostridium, fermented plant fiber and fatty acid mixture is 2-4:15-20:4-8; the fatty acid mixture comprises wall material and fatty acids encapsulated in the wall material.

7. The cat strip according to claim 6, characterized in that: The fermented plant fiber is obtained by fermenting plant fiber with microorganisms, and the mass ratio of plant fiber to microorganisms is 96-99:1-4; The microorganism is selected from at least one of Lactobacillus plantarum, Lactobacillus bulgaricus and Lactobacillus acidophilus; the plant fiber is oat fiber, bamboo shoot fiber and konjac fiber, and the mass ratio of oat fiber, bamboo shoot fiber and konjac fiber is 20-50:5-20:5-10.

8. The cat strip according to claim 6, characterized in that: The wall material is prepared by mixing resistant dextrin, carboxymethyl chitosan and sodium alginate; the fatty acid is a medium-chain fatty acid, and the fatty acid also contains fish oil, soybean lecithin and vitamin E.

9. The cat strip according to claim 6, characterized in that: It also includes complex beneficial ingredients and / or functional substances; The mass ratio of the composite beneficial ingredients to the enzymatic hydrolysate of ethanolic Clostridium is 2-5:2-4; The composite beneficial component is a mixture of taurine, L-carnitine, Poria cocos extract, and resveratrol, and the mass ratio of taurine, L-carnitine, Poria cocos extract, and resveratrol in the mixture is 30-50:10-20:10-20:5-10; The mass ratio of the functional substance to the enzymatic hydrolysate of ethanolic Clostridium is 2:2-4; The functional substance is composed of complex vitamins and minerals, and the mass ratio of the two is 0.1-0.5:0.2-0.5; The compound vitamins are vitamin A, vitamin D, vitamin E and vitamin K; The minerals are copper sulfate, potassium iodide, ferrous sulfate, manganese sulfate, sodium selenite and zinc sulfate.

10. The cat strip according to claim 6, characterized in that: By mass percentage, it is composed of the following ingredients: 40%-60% chicken; 5%-10% krill meal; 2% to 4% ethanolic Clostridium enzymatic hydrolysate; 2%-4% chicken liver; 15-20% fermented plant fiber; 4-8% fatty acid mixture; 2-5% compound beneficial ingredients; 2% functional substances; Add water to make the total mass fraction 100%.

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