Method and system for automatically separating and purifying two insects based on accurate positioning of refractive index

Abstract

The invention relates to the technical field of water quality testing, and discloses a refractive index accurate positioning-based automatic separation and purification method for two insects, which comprises the following steps: preparing and starting an automatic separation and purification device for two insects, the automatic separation and purification device comprising a liquid treatment work station comprising a centrifuge tube, a liquid absorption unit, a liquid relief unit and a controller, the controller controls the liquid suction unit and the liquid transfer unit to automatically complete accurate liquid suction operation and accurate liquid transfer operation of a water sample in the centrifugal tube based on an automatic liquid treatment control program; the liquid suction unit comprises a liquid level sensor, a first flow sensor and a first precision valve unit; the pipetting unit comprises a second flow sensor and a second precision valve unit; collecting and obtaining a water sample, and inputting the water sample into a centrifugal tube of automatic separation and purification equipment for the two insects; accurately positioning and identifying the middle layer of the water sample based on the refractive index, and automatically separating and purifying the two insects based on the middle layer; on the basis of completing automatic separation and purification of the two insects, all pipelines of automatic separation and purification equipment for the two insects are cleaned. A corresponding system is also disclosed.

Description

Technical Field

[0001] The invention relates to the technical field of water quality testing, and in particular to a method and system for automatically separating and purifying two insects based on precise refractive index positioning. Background Art

[0002] Giardia and Cryptosporidium, collectively referred to as "the two worms", are widespread waterborne, chlorine-resistant zoonotic pathogenic microorganisms that can penetrate conventional water treatment processes and enter drinking water systems. Human infection causes diarrhea, dehydration, and even death. There is currently no specific medicine to treat it, and we can only rely on our own immunity. In order to prevent large-scale infection incidents, it is particularly important to detect the two worm indicators in the water. Now the membrane concentration / density gradient separation fluorescent antibody method has been included in GB / T 5750-2023 "Standard Test Methods for Drinking Water", and its overall experimental process includes four steps: sample concentration, separation and purification, staining, and microscopy. At present, the products of the existing technology have been able to realize the intelligence and automation of the two stages of sample concentration and microscopy.

[0003] However, in the separation and purification stage, based on the principle of density gradient separation, whether adding centrifugal medium or extracting the middle layer rich in the two worms, the professionalism and technical skills of the experimenters are very high. A little carelessness will lead to large experimental errors. At the least, the recovery rate will not meet the standard, and at the worst, impurities will be distributed in the middle layer, making it impossible to carry out subsequent overall experiments. The high practical technical threshold of this step has limited the overall development of this method in the industry to a certain extent. Summary of the invention

[0004] The purpose of the present invention is to provide a method and system for automatic separation and purification of two insects based on precise positioning of refractive index, which is used for the automatic separation and purification equipment of the two insects to accurately extract the middle layer, eliminate the microscopic experimental errors caused by the automatic separation and purification of the two insects, ensure that the recovery rate meets the standard, and prevent the phenomenon of impurities being distributed in the middle layer.

[0005] The first aspect of the present invention is to provide a method for automatic separation and purification of two insects based on precise positioning of refractive index, comprising: S1, prepare and start the automatic separation and purification equipment of two insects, the automatic separation and purification equipment of two insects includes a liquid processing workstation, the liquid processing workstation includes a centrifuge tube, a liquid suction unit, a liquid transfer unit and a controller, the controller has an automated liquid processing control program inside, the controller is connected with the liquid suction unit and the liquid transfer unit respectively, and is used to control the liquid suction unit and the liquid transfer unit to automatically complete the precise liquid suction operation and precise liquid transfer operation of the water sample in the centrifuge tube based on the automated liquid processing control program; the liquid suction unit includes a liquid level sensor, a first flow sensor and a first precision valve unit; the liquid transfer unit includes a second flow sensor and a second precision valve unit; S2, collecting and obtaining a water sample, and inputting the water sample into the centrifuge tube of the two-worm automatic separation and purification device; S3, accurately locating and identifying the middle layer of the water sample based on the refractive index, and automatically separating and purifying the two insects based on the middle layer; S4, based on the completion of the automatic separation and purification of the two insects, all pipelines of the automatic separation and purification equipment of the two insects are cleaned.

[0006] Preferably, S3 includes: S31, measuring the refractive index of different layers in the centrifuge tube using a refractometer, wherein the different layers are determined according to a predetermined vertical spacing; S32, determining the refractive index corresponding to the middle layer based on the known density-refractive index relationship of different water samples, and accurately finding the starting and ending positions of the middle layer based on the refractive index corresponding to the middle layer; S33, adding centrifugal medium, and automatically separating and purifying the two insects based on the starting and ending positions of the intermediate layer, wherein the automatic separation and purification of the two insects includes precise aspiration and precise pipetting operations on the water sample in the centrifuge tube.

[0007] Preferably, the vertical spacing ranges from 0.1 to 1 mm.

[0008] Preferably, the S31 includes: (1) Aligning the light source of the refractometer toward the centrifuge tube; (2) measuring the refraction angle of the light generated by the light source when passing through different layers; (3) Calculating the refractive index of different layers in the centrifuge tube according to the refraction angle.

[0009] Preferably, the precise liquid aspiration operation includes: (1) Move the liquid suction unit precisely to the position of the middle layer; (2) accurately aspirating the separation and purification solution containing the two insects in the middle layer; Among them, the precise liquid suction operation is completed by a high-precision suction tool, and the high-precision suction tool includes a suction head controlled by a stepper motor carried by itself, and the stepper motor carried by itself is used to accurately move the liquid suction unit to the position of the middle layer; the suction head is used to accurately suck the separation and purification liquid containing the two insects in the middle layer.

[0010] Preferably, the suction head is a trumpet-shaped suction head.

[0011] Preferably, the precise pipetting operation includes: (1) accurately moving the pipetting unit to the outside of the centrifuge tube and above the container receiving the separation and purification solution containing the two insects; (2) releasing the separated and purified liquid containing the two insects into the container at a predetermined flow rate.

[0012] Preferably, the step of controlling the aspiration unit and the pipetting unit to automatically complete the precise aspiration and pipetting operations of the water sample in the centrifuge tube based on the automated liquid handling control program includes: (1) Before aspirating the liquid, the liquid level of the intermediate layer is monitored in real time by a liquid level sensor and sent to a controller, and the controller controls the suction head to descend synchronously at a controlled speed based on the liquid level of the intermediate layer, so as to ensure accurate aspiration of a quantitative amount of the separated and purified liquid containing the two insects within a fixed time; wherein the controlling the suction head to descend synchronously at a controlled speed based on the liquid level of the intermediate layer comprises: obtaining a real-time measurement value of the liquid level sensor, and adjusting the descending speed of the suction head based on a PID control algorithm and the real-time measurement value of the liquid level sensor; (2) During the liquid aspiration process, the controller automatically controls the suction head to aspirate the separation and purification liquid containing the two insects at a stable flow rate and speed through the first precision valve unit based on the measurement value of the first flow sensor; (3) During the pipetting process, the controller automatically controls the pipette tip to release the separation and purification solution containing the two worms at a stable flow rate and speed through the second precision valve unit based on the measurement value of the second flow sensor.

[0013] Preferably, S4 includes: S41, real-time monitoring of the automatic separation and purification index of the two insects, and determining that the automatic separation and purification of the two insects is completed based on the automatic separation and purification index of the two insects reaching a predetermined threshold; the automatic separation and purification index of the two insects is the recovery rate of the two insects; S42, based on the completion of the automatic separation and purification of the two insects, start the automatic cleaning program and according to the settings of the automatic cleaning program, automatically flush the pipeline, use water, buffer solution or cleaning solution for deep cleaning and drying.

[0014] The second aspect of the present invention is to provide an automatic separation and purification system of two insects based on precise positioning of refractive index, which is used to implement the method of the first aspect, comprising: An automatic separation and purification equipment module (101) for two insects, the automatic separation and purification equipment for two insects comprising a liquid processing workstation, the liquid processing workstation comprising a centrifuge tube, a liquid aspiration unit, a liquid transfer unit and a controller, the controller having an automated liquid processing control program inside, the controller being connected to the liquid aspiration unit and the liquid transfer unit respectively, and being used to control the liquid aspiration unit and the liquid transfer unit to automatically complete precise liquid aspiration and precise liquid transfer operations of the water sample in the centrifuge tube based on the automated liquid processing control program; the liquid aspiration unit comprising a liquid level sensor, a first flow sensor and a first precision valve unit; the liquid transfer unit comprising a second flow sensor and a second precision valve unit; A water sample collection and acquisition module (102) is used to collect and acquire water samples and input the water samples into the centrifuge tube of the two-worm automatic separation and purification device; An intermediate layer positioning module (103) is used to accurately locate and identify the intermediate layer of the water sample based on the refractive index, and automatically separate and purify the two insects based on the intermediate layer; The automatic pipeline cleaning module (104) is used to clean all pipelines of the automatic separation and purification device for the two insects based on the completion of the automatic separation and purification of the two insects.

[0015] A third aspect of the present invention provides an electronic device, comprising a processor and a memory, wherein the memory stores a plurality of instructions, and the processor is configured to read the instructions and execute the method described in the first aspect.

[0016] A fourth aspect of the present invention provides a computer-readable storage medium, wherein the computer-readable storage medium stores a plurality of instructions, and the plurality of instructions can be read by a processor and execute the method described in the first aspect.

[0017] Beneficial effects of the method, system, electronic device and computer-readable storage medium of the present invention: 1. Significantly improve detection efficiency, as shown in: (1) Automated operation process: The equipment can automatically complete a series of operations for the separation and purification of the two insects, such as adding centrifugal media and extracting the intermediate layer, reducing the tedious process of manual operation and greatly saving operation time. Compared with traditional manual detection methods, it can significantly improve detection efficiency. For example, the work of adding separation media, which originally took twenty minutes or even longer to complete, can be completed in a few minutes using automatic equipment.

[0018] (2) Batch processing capability: Some automatic separation and purification equipment for the two insects has the function of batch processing samples and can process multiple samples at the same time. This can greatly improve work efficiency and meet the needs of large-scale testing for water companies, environmental monitoring agencies, etc. that need to conduct large-scale water sample testing.

[0019] 2. Enhance detection accuracy: (1) Accurate control parameters: The automatic equipment uses advanced sensors and control systems to accurately control various parameters in the separation and purification process, such as flow rate, etc. These precise parameter controls can ensure that the separation and purification effects of the two insects are more stable and reliable, reduce errors caused by human factors, and improve the accuracy of the test results.

[0020] (2) Reduce human contamination: During manual operation, samples may be contaminated due to improper operation by operators or environmental factors, which may affect the accuracy of test results. Automatic separation and purification equipment operates in a closed system, which can effectively reduce the contamination of samples by the external environment and reduce the error of test results.

[0021] 3. Reduce labor intensity: (1) Simplified operation steps: The operator only needs to perform simple operations, such as setting parameters and placing samples, and the equipment can automatically complete the subsequent separation and purification work. There is no need for complex manual operations, which greatly reduces the labor intensity of the operator. Especially for those who need to perform two-worm detection work for a long time, the use of automatic equipment can effectively relieve work fatigue.

[0022] (2) No professional skills required: Compared with the traditional method of separating and purifying two worms, which requires operators to have higher professional skills and rich experience, the operation of automatic separation and purification equipment is relatively simple and can be started after simple training, which reduces the professional requirements for operators.

[0023] (IV) It has stable operating performance, is practical, safe and reliable: The automatic separation and purification equipment has undergone rigorous testing and optimization, has stable operating performance, can maintain a good working condition during long-term work, and ensure the smooth progress of the detection work.

[0024] In short, given the many technical advantages such as high degree of automation, high detection accuracy, and strong processing capacity, the automatic separation and purification equipment for the two insects has important application value in the application fields of disease control centers, water supply systems, third-party testing, environmental monitoring, and water quality testing institutions. The promotion and application of this product provides basic tools for a large number of drinking water tests; it provides support for the prevention and control of the "two insects" in the water supply safety guarantee from the source to the tap, and provides guarantees for curbing the transmission of the "two insects" in tap water, which has high social value. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the related technologies, the drawings required for use in the specific embodiments or the related technical descriptions will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0026] Figure 1 A flow chart of a method for automatic separation and purification of two insects based on precise refractive index positioning according to an embodiment of the present invention; Figure 2 This is a flow chart of step S3 of the method for automatic separation and purification of two insects based on precise positioning of refractive index according to an embodiment of the present invention; Figure 3 This is a flow chart of step S4 of the method for automatic separation and purification of two insects based on precise positioning of refractive index according to an embodiment of the present invention; Figure 4 A schematic diagram of the principle structure of a system for automatic separation and purification of two insects based on precise refractive index positioning according to an embodiment of the present invention; Figure 5 A structural diagram of an electronic device provided according to an embodiment of the present invention. DETAILED DESCRIPTION

[0027] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0029] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances. Embodiment 1

[0030] See also Figure 1 This embodiment provides a method for automatic separation and purification of two insects based on precise positioning of refractive index, comprising: S1, prepare and start the automatic separation and purification equipment of two insects, the automatic separation and purification equipment of two insects includes a liquid processing workstation, the liquid processing workstation includes a centrifuge tube, a liquid suction unit, a liquid transfer unit and a controller, the controller has an automated liquid processing control program inside, the controller is connected with the liquid suction unit and the liquid transfer unit respectively, and is used to control the liquid suction unit and the liquid transfer unit to automatically complete the precise liquid suction operation and precise liquid transfer operation of the water sample in the centrifuge tube based on the automated liquid processing control program; the liquid suction unit includes a liquid level sensor, a first flow sensor and a first precision valve unit; the liquid transfer unit includes a second flow sensor and a second precision valve unit; S2, collecting and obtaining a water sample, and inputting the water sample into the centrifuge tube of the two-worm automatic separation and purification device; S3, accurately locating and identifying the middle layer of the water sample based on the refractive index, and automatically separating and purifying the two insects based on the middle layer; like Figure 2 As shown, as a preferred implementation, S3 includes: S31, using a refractometer to measure the refractive index of different layers in the centrifuge tube, wherein the different layers are determined according to a predetermined vertical spacing. In this embodiment, the vertical spacing is in the range of 0.1-1 mm; As a preferred implementation, the S31 includes: (1) Aligning the light source of the refractometer toward the centrifuge tube; (2) measuring the refraction angle of the light generated by the light source when passing through different layers; (3) Calculating the refractive index of different layers in the centrifuge tube according to the refraction angle.

[0031] In this embodiment, in density gradient centrifugation, layers of different densities may have different refractive indices, so the interface between the layers can be identified by the change in refractive index, and then the position of the intermediate layer can be determined. A refractometer is used to measure the refractive index of different layers in the centrifuge tube. The light source of the refractometer is aimed at the centrifuge tube, and the refraction angle of the light when passing through different layers is measured. The refractometer calculates the refractive index based on the refraction angle.

[0032] Working principle: Refractive index is the ratio of the speed of light in a vacuum to the speed of light in the medium. The higher the refractive index of a material, the stronger its ability to refract incident light. The higher the refractive index, the thinner the lens, that is, the same thickness at the center of the lens, the same degree and the same material, the lens with a high refractive index is thinner at the edge than the lens with a low refractive index. The refractive index is closely related to the electromagnetic properties of the medium. When a relatively light-dense medium is incident on a relatively light-sparse medium, and the incident angle is greater than the critical angle, total reflection can occur. In density gradient centrifugation, the essential reason why layers of different densities may have different refractive indices is that the refractive index is also closely related to the arrangement of ions (physical and chemical manifestations of solutions containing two worms). Isotropic optical materials, such as amorphous (amorphous body) and cubic crystals, have only one refractive index; when light enters an inhomogeneous medium, it is generally divided into two waves with mutually perpendicular vibration directions and different propagation speeds, each of which has two refracted rays, forming the so-called double refraction. The refractive index of the two refracted rays parallel to the incident plane is called the ordinary light refractive index; no matter how the incident angle of the incident light changes, it is always a constant and obeys the law of refraction. The refractive index of the other ray perpendicular to the incident plane changes with the direction of the incident light, which is called the extraordinary light refractive index; it does not obey the law of refraction. When light is incident along the optical axis of the crystal, only the ordinary light refractive index exists. When it is incident perpendicular to the optical axis, the extraordinary light refractive index reaches the maximum value, which is the characteristic of the material. Therefore, the extraordinary light refractive index is larger along the direction where the crystal is more densely packed.

[0033] S32, determining the refractive index corresponding to the middle layer based on the known density-refractive index relationship of different water samples, and accurately finding the starting and ending positions of the middle layer based on the refractive index corresponding to the middle layer; S33, adding centrifugal medium, and automatically separating and purifying the two insects based on the starting and ending positions of the intermediate layer, wherein the automatic separation and purification of the two insects includes precise liquid aspiration and precise liquid transfer operations on the water sample in the centrifuge tube; As a preferred embodiment, the precise liquid aspiration operation includes: (1) Move the liquid suction unit precisely to the position of the middle layer; (2) Accurately aspirate the separation and purification solution containing the two insects in the middle layer.

[0034] As a preferred embodiment, the precise liquid aspiration operation is completed by a high-precision suction tool, and the high-precision suction tool includes a suction head controlled by a stepper motor carried by the tool, and the suction unit is precisely moved to the position of the middle layer by the stepper motor carried by the tool, and the movement accuracy can reach the millimeter level or even higher; the suction head is used to accurately absorb the separated and purified liquid containing the two insects in the middle layer; wherein the shape of the suction head is designed to facilitate accurate extraction. For example, the present embodiment adopts a trumpet-shaped suction head, which better fits the middle layer during the suction process and reduces interference with the upper and lower layer materials.

[0035] As a preferred embodiment, the precise pipetting operation includes: (1) accurately moving the pipetting unit to the outside of the centrifuge tube and above the container receiving the separation and purification solution containing the two insects; (2) releasing the separated and purified liquid containing the two insects into the container at a predetermined flow rate.

[0036] As a preferred embodiment, the automatic liquid handling control program is used to control the aspiration unit and the pipetting unit to automatically complete the precise aspiration and precise pipetting operations of the water sample in the centrifuge tube, including: (1) Before aspirating the liquid, the liquid level of the intermediate layer is monitored in real time by a liquid level sensor and sent to a controller, and the controller controls the suction head to descend synchronously at a controlled speed based on the liquid level of the intermediate layer, so as to ensure accurate aspiration of a quantitative amount of the separated and purified liquid containing the two insects within a fixed time; wherein, the control of the suction head to descend synchronously at a controlled speed based on the liquid level of the intermediate layer includes: obtaining a real-time measurement value of the liquid level sensor, and adjusting the descending speed of the suction head based on a PID control algorithm and the real-time measurement value of the liquid level sensor, so as to avoid aspirating materials in other layers, thereby achieving efficient and accurate liquid transfer; (2) During the liquid aspiration process, the controller automatically controls the suction head to aspirate the separation and purification liquid containing the two insects at a stable flow rate and speed through the first precision valve unit based on the measurement value of the first flow sensor, thereby avoiding mixing of substances in other layers due to excessively fast aspiration speed; (3) During the pipetting process, the controller automatically controls the pipette tip to release the separation and purification solution containing the two worms at a stable flow rate and speed through the second precision valve unit based on the measurement value of the second flow sensor.

[0037] S4, based on the completion of the automatic separation and purification of the two insects, all pipelines of the automatic separation and purification equipment of the two insects are cleaned.

[0038] like Figure 3 As shown, as a preferred embodiment, the S4 includes: S41, monitoring the automatic separation and purification index of the two insects in real time, and determining that the automatic separation and purification of the two insects is completed based on the automatic separation and purification index of the two insects reaching a predetermined threshold; In this embodiment, the automatic separation and purification index of the two insects is the recovery rate of the two insects.

[0039] S42, based on the completion of the automatic separation and purification of the two insects, start the automatic cleaning program and according to the settings of the automatic cleaning program, automatically flush the pipeline, use water, buffer solution or cleaning solution for deep cleaning and drying.

[0040] In this embodiment, the method and system are equipped with an automatic cleaning system, which can thoroughly clean and dry the pipeline according to the program settings to effectively prevent cross contamination. The system supports a comprehensive cleaning process, including automatic rinsing, drying, and deep cleaning using water or a special buffer solution (cleaning solution). In addition, the system also provides delayed start, pause, and resume functions to ensure flexibility and convenience of operation. The complete collection function allows the cleaned samples to be collected safely and accurately for subsequent use. Embodiment 2

[0041] like Figure 4 As shown, this embodiment provides an automatic separation and purification system of two insects based on precise positioning of refractive index, which is used to implement the method of embodiment 1, including: The automatic separation and purification equipment module 101 of two insects comprises a liquid processing workstation, the liquid processing workstation comprises a centrifuge tube, a liquid suction unit, a liquid transfer unit and a controller, the controller has an automated liquid processing control program inside, the controller is connected to the liquid suction unit and the liquid transfer unit respectively, and is used to control the liquid suction unit and the liquid transfer unit to automatically complete the precise liquid suction operation and precise liquid transfer operation of the water sample in the centrifuge tube based on the automated liquid processing control program; the liquid suction unit comprises a liquid level sensor, a first flow sensor and a first precision valve unit; the liquid transfer unit comprises a second flow sensor and a second precision valve unit; A water sample collection and acquisition module 102 is used to collect and acquire water samples and input the water samples into the centrifuge tube of the two-worm automatic separation and purification device; An intermediate layer positioning module 103 is used to accurately locate and identify the intermediate layer of the water sample based on the refractive index, and automatically separate and purify the two insects based on the intermediate layer; The automatic pipeline cleaning module 104 is used to clean all pipelines of the automatic separation and purification device of the two insects based on the completion of the automatic separation and purification of the two insects.

[0042] The present invention also provides a memory storing a plurality of instructions, wherein the instructions are used to implement the method as in the first embodiment.

[0043] like Figure 5 As shown, the present invention also provides an electronic device, including a processor 301 and a memory 302 connected to the processor 301, the memory 302 stores a plurality of instructions, and the instructions can be loaded and executed by the processor so that the processor can execute the method as in the first embodiment.

[0044] Through the description of the above implementation methods, those skilled in the art can clearly understand that the above embodiments can be implemented by software, or by means of software plus necessary general hardware platforms. Based on such understanding, the technical solutions of the above embodiments can be embodied in the form of software products, which can be stored in a non-volatile storage medium (which can be a CD-ROM, a USB flash drive, a mobile hard disk, etc.), including several instructions for enabling a computer device (which can be a personal computer, a server, or a network device, etc.) to execute the methods described in the various embodiments of the present invention.

[0045] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for automatic separation and purification of two insects based on precise positioning of refractive index, characterized in that: include: S1, prepare and start the automatic separation and purification equipment of two insects, the automatic separation and purification equipment of two insects includes a liquid processing workstation, the liquid processing workstation includes a centrifuge tube, a liquid suction unit, a liquid transfer unit and a controller, the controller has an automated liquid processing control program inside, the controller is connected with the liquid suction unit and the liquid transfer unit respectively, and is used to control the liquid suction unit and the liquid transfer unit to automatically complete the precise liquid suction operation and precise liquid transfer operation of the water sample in the centrifuge tube based on the automated liquid processing control program; the liquid suction unit includes a liquid level sensor, a first flow sensor and a first precision valve unit; the liquid transfer unit includes a second flow sensor and a second precision valve unit; S2, collecting and obtaining a water sample, and inputting the water sample into the centrifuge tube of the two-worm automatic separation and purification device; S3, accurately locating and identifying the middle layer of the water sample based on the refractive index, and automatically separating and purifying the two insects based on the middle layer; S4, based on the completion of the automatic separation and purification of the two insects, all pipelines of the automatic separation and purification equipment of the two insects are cleaned.

2. The method for automatic separation and purification of two insects based on precise refractive index positioning according to claim 1 is characterized in that: The S3 includes: S31, measuring the refractive index of different layers in the centrifuge tube using a refractometer, wherein the different layers are determined according to a predetermined vertical spacing; S32, determining the refractive index corresponding to the middle layer based on the known density-refractive index relationship of different water samples, and accurately finding the starting and ending positions of the middle layer based on the refractive index corresponding to the middle layer; S33, adding centrifugal medium, and automatically separating and purifying the two insects based on the starting and ending positions of the intermediate layer, wherein the automatic separation and purification of the two insects includes precise aspiration and precise pipetting operations on the water sample in the centrifuge tube.

3. The method for automatic separation and purification of two insects based on precise refractive index positioning according to claim 2 is characterized in that: The vertical spacing ranges from 0.1 to 1 mm.

4. The method for automatic separation and purification of two insects based on precise refractive index positioning according to claim 3 is characterized in that: The S31 includes: (1) Aligning the light source of the refractometer toward the centrifuge tube; (2) measuring the refraction angle of the light generated by the light source when passing through different layers; (3) Calculating the refractive index of different layers in the centrifuge tube according to the refraction angle.

5. The method for automatic separation and purification of two insects based on precise refractive index positioning according to claim 4 is characterized in that: The precise liquid aspiration operation includes: (1) Move the liquid suction unit precisely to the position of the middle layer; (2) accurately aspirating the separation and purification solution containing the two insects in the middle layer; Among them, the precise liquid suction operation is completed by a high-precision suction tool, and the high-precision suction tool includes a suction head controlled by a stepper motor carried by itself, and the stepper motor carried by itself is used to accurately move the liquid suction unit to the position of the middle layer; the suction head is used to accurately suck the separation and purification liquid containing the two insects in the middle layer.

6. The method for automatic separation and purification of two insects based on precise refractive index positioning according to claim 5 is characterized in that: The suction head is a trumpet-shaped suction head.

7. The method for automatic separation and purification of two insects based on precise refractive index positioning according to claim 6 is characterized in that: The precise pipetting operation includes: (1) accurately moving the pipetting unit to the outside of the centrifuge tube and above the container receiving the separation and purification solution containing the two insects; (2) releasing the separated and purified liquid containing the two insects into the container at a predetermined flow rate.

8. The method for automatic separation and purification of two insects based on precise refractive index positioning according to claim 7 is characterized in that: The method of controlling the aspiration unit and the pipetting unit based on the automated liquid handling control program to automatically complete the precise aspiration operation and precise pipetting operation of the water sample in the centrifuge tube includes: (1) Before aspirating the liquid, the liquid level of the intermediate layer is monitored in real time by a liquid level sensor and sent to a controller, and the controller controls the suction head to descend synchronously at a controlled speed based on the liquid level of the intermediate layer, so as to ensure accurate aspiration of a quantitative amount of the separated and purified liquid containing the two insects within a fixed time; wherein the controlling the suction head to descend synchronously at a controlled speed based on the liquid level of the intermediate layer comprises: obtaining a real-time measurement value of the liquid level sensor, and adjusting the descending speed of the suction head based on a PID control algorithm and the real-time measurement value of the liquid level sensor; (2) During the liquid aspiration process, the controller automatically controls the suction head to aspirate the separation and purification liquid containing the two insects at a stable flow rate and speed through the first precision valve unit based on the measurement value of the first flow sensor; (3) During the pipetting process, the controller automatically controls the pipette tip to release the separation and purification solution containing the two worms at a stable flow rate and speed through the second precision valve unit based on the measurement value of the second flow sensor.

9. The method for automatic separation and purification of two insects based on precise refractive index positioning according to claim 8, characterized in that: The S4 includes: S41, real-time monitoring of the automatic separation and purification index of the two insects, and determining that the automatic separation and purification of the two insects is completed based on the automatic separation and purification index of the two insects reaching a predetermined threshold; the automatic separation and purification index of the two insects is the recovery rate of the two insects; S42, based on the completion of the automatic separation and purification of the two insects, start the automatic cleaning program and according to the settings of the automatic cleaning program, automatically flush the pipeline, use water, buffer solution or cleaning solution for deep cleaning and drying.

10. An automatic separation and purification system for two insects based on precise positioning of refractive index, used for implementing any method described in claims 1-9, characterized in that: include: An automatic separation and purification equipment module (101) for two insects, the automatic separation and purification equipment for two insects comprising a liquid processing workstation, the liquid processing workstation comprising a centrifuge tube, a liquid aspiration unit, a liquid transfer unit and a controller, the controller having an automated liquid processing control program inside, the controller being connected to the liquid aspiration unit and the liquid transfer unit respectively, and being used to control the liquid aspiration unit and the liquid transfer unit to automatically complete precise liquid aspiration and precise liquid transfer operations of the water sample in the centrifuge tube based on the automated liquid processing control program; the liquid aspiration unit comprising a liquid level sensor, a first flow sensor and a first precision valve unit; the liquid transfer unit comprising a second flow sensor and a second precision valve unit; A water sample collection and acquisition module (102) is used to collect and acquire water samples and input the water samples into the centrifuge tube of the two-worm automatic separation and purification device; An intermediate layer positioning module (103) is used to accurately locate and identify the intermediate layer of the water sample based on the refractive index, and automatically separate and purify the two insects based on the intermediate layer; The automatic pipeline cleaning module (104) is used to clean all pipelines of the automatic separation and purification device for the two insects based on the completion of the automatic separation and purification of the two insects.

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