Double-layer essence oil with antioxidant and firming functions and preparation method of double-layer essence oil

Abstract

The invention discloses antioxidant and firming double-layer essence oil and a preparation method thereof, and belongs to the technical field of cosmetics. The double-layer essence oil contains an anti-oxidation and firming composition, the composition comprises the following components: crambe abyssinica seed oil, cynara plantago seed oil, a hop extract and a humulus lupulus extract, and through the synergistic effect of the four components, the double-layer essence oil has good anti-oxidation and skin firming effects; and the double-layer essence oil prepared by the preparation method disclosed by the invention is easier to absorb by skin.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, and in particular to a double-layer essence oil with anti-oxidation and firming effects and a preparation method thereof. Background Art

[0002] There are many factors that cause skin wrinkling and aging, such as environmental pollution, environmental factors, life and work pressure, aging, etc. Among them, environmental factors such as ultraviolet radiation, long-term exposure of the skin to ultraviolet radiation causes photoaging, and the photoaging of the skin is due to the increase of free radicals in the skin, which damages the biofilm, promotes the cross-linking and polymerization of elastic fibers, and accelerates skin aging. There are many types of skin care products for anti-aging on the market. Some of them are vitamin-based antioxidants such as vitamin E, but this type of antioxidant has poor stability and its application is limited. In recent years, studies have found that some plants or Chinese herbal medicines contain antioxidant ingredients, which can delay skin aging, tighten the skin, and play a role in wrinkle removal and anti-aging. However, the anti-aging effect of existing cosmetics containing plants or Chinese herbal medicines is unstable. Therefore, based on the above problems, it is urgent to provide an essential oil product with good antioxidant and firming effects and strong stability. Summary of the invention

[0003] The purpose of the present invention is to provide a double-layer essential oil with antioxidant and firming effects and a preparation method thereof. The active substance of the product adopts natural plant extracts and has high safety. The double-layer essential oil obtained by the preparation method of the present invention has better antioxidant and skin firming effects.

[0004] In order to achieve the above object, the present invention adopts the following technical solutions:

[0005] In a first aspect, the present invention provides an antioxidant firming composition, comprising the following components: Camelina officinalis seed oil, Echium plantain seed oil, hops extract, and Scutellaria baicalensis extract.

[0006] Preferably, the antioxidant firming composition consists of the following components in parts by weight:

[0007] 3-6 parts of deep sea camellia oil;

[0008] 3-6 parts of Echium seed oil;

[0009] 3-6 parts of hop extract;

[0010] 3-6 parts of Radix Codonopsis pilosulae extract.

[0011] Furthermore, the antioxidant firming composition is composed of the following components in parts by weight:

[0012] 3-4 parts of deep sea camellia oil;

[0013] 3-4 parts of Echium seed oil;

[0014] 3-4 parts of hop extract;

[0015] 3-4 parts of Radix Angelicae Pubescentis extract.

[0016] Optimally, the antioxidant firming composition consists of the following components in parts by weight:

[0017] 4 parts of deep sea camellia oil;

[0018] 4 parts of Echium seed oil;

[0019] 4 parts of hop extract;

[0020] 4 parts of Radix Angelicae Pubescentis extract.

[0021] In a second aspect, the present invention provides a double-layer essence oil, which is composed of the following components in percentage by mass: 1-5wt% of an antioxidant firming composition, 35-40wt% of isohexadecane, 2-4wt% of C13-16 isoparaffin, 0.1-0.3wt% of caprylic / capric triglyceride, 1-5wt% of coconut oil caprylate / caprate, 3-6wt% of butylene glycol, 0.1-0.5wt% of 1,2-hexanediol, 0.1-0.3wt% of parahydroxyacetophenone, 0.1-0.5wt% of potassium methoxysalicylate, and the balance is deionized water.

[0022] Preferably, the double-layer essential oil is composed of the following components in percentage by mass:

[0023] 1-3 wt% of antioxidant firming composition;

[0024] Isohexadecane 35-38wt%;

[0025] C13-16 isoparaffin 2-3 wt%;

[0026] Caprylic / capric triglyceride 0.1-0.2wt%;

[0027] Coconut oil alcohol-caprylate / caprate 1-3wt%;

[0028] Butanediol 3-4wt%;

[0029] 1,2-Hexanediol 0.1-0.3wt%;

[0030] p-Hydroxyacetophenone 0.1-0.2wt%;

[0031] Potassium methoxysalicylate 0.1-0.3wt%;

[0032] The balance is deionized water.

[0033] Optimally, the double-layer essential oil is composed of the following components in percentage by mass:

[0034] 3 wt% of antioxidant firming composition;

[0035] Isohexadecane 38wt%;

[0036] C13-16 isoparaffin 3wt%;

[0037] Caprylic / capric triglyceride 0.2wt%;

[0038] Coconut oil alcohol-caprylate / caprate 3wt%;

[0039] Butanediol 4wt%;

[0040] 1,2-Hexanediol 0.3wt%;

[0041] p-Hydroxyacetophenone 0.2wt%;

[0042] Potassium methoxysalicylate 0.3wt%;

[0043] The balance is deionized water.

[0044] In a third aspect, the present invention provides a method for preparing a double-layer essential oil, the specific steps of which are as follows:

[0045] S1. Place deep sea camellia seed oil, echium plantaginis seed oil, coconut oil caprylate / caprate, isohexadecane, C13-16 isoparaffin, caprylic / capric triglyceride in an emulsifier, heat to 50-55° C., mix well, and obtain phase A;

[0046] S2. Butanediol, 1,2-hexanediol, p-hydroxyacetophenone and deionized water are placed in an emulsifying pot, heated to 75-80°C, mixed evenly, cooled to 45-48°C, potassium methoxysalicylate, hop extract and yellow heart extract are added and mixed and stirred evenly to obtain phase B;

[0047] S3. Mix phase A and phase B, sterilize, fill, and seal for storage.

[0048] Compared with the prior art, the present invention has the following beneficial effects: the antioxidant firming composition of the present invention comprises deep sea camellia seed oil, echium plantaginis seed oil, hop extract and yellow calendula extract, wherein deep sea camellia seed oil and yellow calendula extract provide vitamin E and flavonoids, capture free radicals and reduce oxidative stress; echium plantaginis seed oil and hop extract convert ALA and SDA into EPA and α-acid and β-acid, thereby enhancing the overall antioxidant effect; echium plantaginis seed oil indirectly improves the nutrition and oxygen supply of the skin by promoting cardiovascular health, while Deep-sea camellia oil forms a protective film directly on the skin surface, and the synergistic effect of the two can effectively enhance the firmness and elasticity of the skin; the anti-inflammatory and repairing effects of the yellow caltrop extract, combined with the antioxidant capacity of the hops extract, can reduce inflammatory responses and UV damage to the skin, and promote skin health; the present invention innovatively uses deep-sea camellia oil, echium plantaginis seed oil, hops extract, and yellow caltrop extract together. Experiments have found that the combined use of the above four ingredients has significant synergistic effects in anti-oxidation and skin firming, and has excellent production and application value. DETAILED DESCRIPTION

[0049] In order to better understand the present invention, the present invention is further described below in conjunction with specific examples, wherein the terms used in the examples are for describing specific embodiments and do not constitute limitations on the scope of protection of the present invention.

[0050] The experimental methods without specific conditions in the following tests are usually carried out under conventional conditions or the conditions recommended by the manufacturer. Unless otherwise specified, percentages and parts are calculated by weight.

[0051] Some raw materials and their sources are as follows:

[0052] Camelina purse seed oil: purchased from Guangzhou Chenyu Biotechnology Co., Ltd.;

[0053] Echium plantain seed oil: purchased from Guangzhou Artemisia Biotechnology Co., Ltd.;

[0054] Hops extract: purchased from Shaanxi Hengling Natural Biological Products Co., Ltd.;

[0055] Huang Xixin extract: purchased from Shaanxi Yinuo Biotechnology Co., Ltd.;

[0056] The weight fractions of the components in the antioxidant firming compositions of the embodiments of the present invention and the comparative examples are shown in Table 1 below:

[0057] Table 1 Mass fraction of each component in the antioxidant firming composition

[0058]

[0059] Note: "" means no addition, the missing mass parts are replaced by an equal amount of deionized water.

[0060] A double-layer essential oil, the mass percentages of its components are shown in Table 2 below.

[0061] Table 2 Double-layer essential oil mass percentage

[0062]

[0063]

[0064]

[0065]

[0066] The preparation methods of the above essential oils 1, 4, 5, essential oils ①-⑧ and essential oil blanks are as follows:

[0067] S1. Place deep sea camellia seed oil, echium plantaginis seed oil, coconut oil caprylate / caprate, isohexadecane, C13-16 isoparaffin, caprylic / capric triglyceride in an emulsifier, heat to 53° C., mix well, and obtain phase A;

[0068] S2. Butanediol, 1,2-hexanediol, p-hydroxyacetophenone and deionized water are placed in an emulsifying pot, heated to 80°C, mixed evenly, cooled to 47°C, potassium methoxysalicylate, hop extract and yellow heart extract are added and mixed and stirred evenly to obtain phase B;

[0069] S3. Mix phase A and phase B, sterilize, fill, and seal for storage.

[0070] The preparation method of essential oil 2 is as follows:

[0071] S1. Place deep sea camellia seed oil, echium plantaginis seed oil, coconut oil caprylate / caprate, isohexadecane, C13-16 isoparaffin, caprylic / capric triglyceride in an emulsifier, heat to 50° C., mix well, and obtain phase A;

[0072] S2. Butanediol, 1,2-hexanediol, p-hydroxyacetophenone and deionized water are placed in an emulsifying pot, heated to 80°C, mixed evenly, cooled to 45°C, potassium methoxysalicylate, hop extract and yellow heart extract are added and mixed and stirred evenly to obtain phase B;

[0073] S3. Mix phase A and phase B, sterilize, fill, and seal for storage.

[0074] The preparation method of essential oil 3 is as follows:

[0075] S1. Place deep sea camellia seed oil, echium plantaginis seed oil, coconut oil caprylate / caprate, isohexadecane, C13-16 isoparaffin, caprylic / capric triglyceride in an emulsifier, heat to 55° C., mix well, and obtain phase A;

[0076] S2. Butanediol, 1,2-hexanediol, p-hydroxyacetophenone and deionized water are placed in an emulsifying pot, heated to 75°C, mixed evenly, cooled to 48°C, potassium methoxysalicylate, hop extract and yellow heart extract are added and mixed and stirred evenly to obtain phase B;

[0077] S3. Mix phase A and phase B, sterilize, fill, and seal for storage.

[0078] Essential Oil ⑨:

[0079] The formula used in essence oil 9 is the same as that of essence oil 1. The difference from essence oil 1 is that its preparation method is as follows:

[0080] Potassium methoxysalicylate, hops extract, yellow calendula extract, deep sea camellia seed oil, plantain echium seed oil, coconut oil caprylate / caprate, isohexadecane, C13-16 isoparaffin, caprylic / capric triglyceride, butylene glycol, 1,2-hexanediol, p-hydroxyacetophenone and deionized water are placed in an emulsifying pot, first heated to 55°C and mixed evenly, then heated to 80°C and mixed evenly, cooled to 47°C, mixed and stirred evenly, sterilized, filled, and sealed for storage.

[0081] Essential Oil ⑩:

[0082] Essence Oil ⑩ differs from Essence Oil 1 in that it contains only Phase A.

[0083] Essential Oil

[0084] Essential Oil Unlike Essence Oil 1, only Phase B is included.

[0085] Experiment 1: Human safety test

[0086] 1-1. Human skin patch test

[0087] Tested substances: Essence oil 1-5 and essence oil

[0088] Subject selection: Select healthy volunteers aged 21-57 years old, who voluntarily participate and sign the informed consent form and can complete the test as required during the test period. Each group has 30 subjects.

[0089] Experimental method: Select qualified patch equipment and closed patch test method, absorb 0.020mL-0.025mL (liquid) of the test substance and place it in the patch tester, apply it to the subject's back or flexor side of the forearm with low-allergenic tape, remove the test substance after 24 hours, observe and record the skin reaction 0.5, 24, and 48 hours after removal.

[0090] Result analysis: The grading of adverse skin reactions is determined according to the grading of adverse skin reactions in human trial tests specified in the "Technical Specifications for Safety of Cosmetics" (2015 edition) issued by the State Food and Drug Administration. The grading standards are shown in Table 1-1 and the results are shown in Table 1-2.

[0091] Table 1-1 Classification criteria for adverse skin reactions

[0092]

[0093]

[0094] Table 1-2 Human skin patch test results

[0095]

[0096]

[0097] 1-2. Human test on sensitive skin

[0098] Tested substances: Essence oil 1-5 and essence oil

[0099] Subject selection: healthy volunteers aged 21-44 years old, who meet the requirements of lactic acid sting test, voluntarily participate and sign the informed consent, and can complete the test as required during the test period are selected. Each group has 30 subjects.

[0100] Experimental method: Volunteers randomly divided both sides of their faces into test side and control side. After cleansing, the product was evenly applied to the facial skin of the test side according to the product usage instructions, and no product was used on the control side. After 28 days of trial, the volunteers reported local skin reactions. If there were any adverse reactions, dermatologists would evaluate the adverse reactions.

[0101] Result analysis: The grading of adverse skin reactions is determined according to the grading of adverse skin reactions in human trial tests specified in the "Technical Specifications for Safety of Cosmetics" (2015 edition) issued by the State Food and Drug Administration. The grading standards are shown in Table 1-1 and the results are shown in Table 1-3.

[0102] Table 1-3 Results of human trial tests

[0103]

[0104] Based on the results of human skin patch test and sensitive skin suitability test, during the test period, essence oil 1-5 and essence oil None of the subjects experienced adverse skin reactions, indicating that essential oils 1-5 and essential oil It has good safety, is mild and non-irritating, and is suitable for people with sensitive skin.

[0105] Test 2: Antioxidant test determination

[0106] Sample solution: The sample solution is divided into a sample group and a positive control group; the sample group is composed of essence oil 1-5, essence oil The blank essential oil was prepared into an aqueous solution with a concentration of 5 mg / mL, the positive control group was a Vc aqueous solution with a concentration of 1 mg / L, and the solvent used in the sample solution was sterile deionized water.

[0107] Note: Before use, the sample group should be emulsified using an AES3300 fully automatic multi-channel emulsifier (Nanjing Baode Biotechnology Co., Ltd.), and the test should be completed within 60 minutes after the sample is emulsified.

[0108] Test steps:

[0109] 1. Take 2mL of sample solution and 2mL of 2*10 -4 1. 1 mol / L DPPH anhydrous ethanol solution was mixed (A1);

[0110] 2. Take 2mL of sterile deionized water and 2mL of 2*10 -4 1 mol / L DPPH anhydrous ethanol solution was mixed (A2);

[0111] 3. Take 2 mL of anhydrous ethanol and mix it with the sample solution (A3);

[0112] 4. After A1, A2, and A3 have reacted for 40 minutes, measure the absorbance of A1, A2, and A3 at 517 nm.

[0113] The calculation formula of clearance rate is: Clearance rate (%) = [1-(A1-A3) / A2]*100%

[0114] The experimental results are shown in Table 4.

[0115] Test 3: Determination of firmness test

[0116] Sample solution: The sample solution is divided into a sample group and a positive control group; the sample group is essence oil 1-5, essence oil and blank essential oil, which were prepared into 0.5 mg / L aqueous solution using sterile deionized water; the positive control group was TGF-β1, with a concentration of 100 ng / mL.

[0117] Test method: Refer to T / SHRH 031-2020 "Test of firming and anti-wrinkle efficacy of cosmetics-determination of type I collagen content in fibroblasts in vitro" to test essence oil 1-5, essence oil The effects of the blank essential oil on promoting the synthesis of type I collagen were tested at a concentration of 0.5 mg / L. The test results are shown in Table 4.

[0118] Table 4 Antioxidant and firming effects

[0119]

[0120]

[0121] Note: “*”P<0.05.

[0122] Comparing the essence oils 1-5, the results show that the essence oil 3 has the best antioxidant effect and the best effect of improving type I collagen, but compared with the addition amount of the essence oil 3 composition, the essence oil 1 has the best cost performance, so the essence oil 1 is selected as the best embodiment.

[0123] Comparison of essence oil 1 with essence oils ①-⑧ shows that the four compositions provided by the present invention have significant synergistic effects. The results show that the preparation method defined in the present invention can effectively enhance the antioxidant effect of the essence oil and the effect of synthesizing type I collagen. It can be seen that compared with using phases AB alone, using them together has better antioxidant effects and the effect of promoting the synthesis of type I collagen.

[0124] The embodiments described above are part of the embodiments of the present application, rather than all of the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the present application for protection, but merely represents the selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present application.

Claims

1. An antioxidant firming composition, characterized in that: The antioxidant firming composition is composed of the following components in parts by weight: 3-6 parts of deep sea camellia oil; 3-6 parts of Echium seed oil; 3-6 parts of hop extract; 3-6 parts of Radix Codonopsis pilosulae extract.

2. The antioxidant firming composition according to claim 1, characterized in that: The antioxidant firming composition is composed of the following components in parts by weight: 3-4 parts of deep sea camellia oil; 3-4 parts of Echium seed oil; 3-4 parts of hop extract; 3-4 parts of Radix Angelicae Pubescentis extract.

3. The antioxidant firming composition according to claim 1, characterized in that: The antioxidant firming composition is composed of the following components in parts by weight: 4 parts of deep sea camellia oil; 4 parts of Echium seed oil; 4 parts of hop extract; 4 parts of Radix Angelicae Pubescentis extract.

4. A double-layer essence oil containing the antioxidant firming composition of claim 1, characterized in that: The double-layer essential oil is composed of the following components in percentage by weight: 1-5 wt% of antioxidant firming composition; Isohexadecane 35-40wt%; C13-16 isoparaffin 2-4 wt%; Caprylic / capric triglyceride 0.1-0.3wt%; Coconut oil alcohol-caprylate / caprate 1-5wt%; Butanediol 3-6wt%; 1,2-Hexanediol 0.1-0.5wt%; p-Hydroxyacetophenone 0.1-0.3wt%; Potassium methoxysalicylate 0.1-0.5wt%; The balance is deionized water.

5. The double-layer essential oil according to claim 4, characterized in that: The double-layer essential oil is composed of the following components in percentage by weight: 1-3 wt% of antioxidant firming composition; Isohexadecane 35-38wt%; C13-16 isoparaffin 2-3 wt%; Caprylic / capric triglyceride 0.1-0.2wt%; Coconut oil alcohol-caprylate / caprate 1-3wt%; Butanediol 3-4wt%; 1,2-Hexanediol 0.1-0.3wt%; p-Hydroxyacetophenone 0.1-0.2wt%; Potassium methoxysalicylate 0.1-0.3wt%; The balance is deionized water.

6. The double-layer essential oil according to claim 4, characterized in that: The double-layer essential oil is composed of the following components in percentage by weight: 3 wt% of antioxidant firming composition; Isohexadecane 38wt%; C13-16 isoparaffin 3wt%; Caprylic / capric triglyceride 0.2wt%; Coconut oil alcohol-caprylate / caprate 3wt%; Butanediol 4wt%; 1,2-Hexanediol 0.3wt%; p-Hydroxyacetophenone 0.2wt%; Potassium methoxysalicylate 0.3wt%; The balance is deionized water.

7. The double-layer essence oil according to any one of claims 4 to 6, characterized in that: The preparation method of the double-layer essential oil comprises the following steps: S1. Place deep sea camellia seed oil, echium plantaginis seed oil, coconut oil caprylate / caprate, isohexadecane, C13-16 isoparaffin, caprylic / capric triglyceride in an emulsifier, heat to 50-55° C., mix well, and obtain phase A; S2. Butanediol, 1,2-hexanediol, p-hydroxyacetophenone and deionized water are placed in an emulsifying pot, heated to 75-80°C, mixed evenly, cooled to 45-48°C, potassium methoxysalicylate, hop extract and yellow heart extract are added and mixed and stirred evenly to obtain phase B; S3. Mix phase A and phase B, sterilize, fill, and seal for storage.

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