Double-layer essential oil with anti-oxidation and firming properties and preparation method thereof

Abstract

The present invention discloses a double-layer essential oil with antioxidant and firming properties and a preparation method thereof, belonging to the field of cosmetics technology. The double-layer essential oil contains an antioxidant and firming composition comprising the following ingredients: Capsella oleracea seed oil, Echium plantain seed oil, hops extract, and Rhizoma Cimicifugae extract. Through the synergistic effect of these four components, the double-layer essential oil exhibits excellent antioxidant and skin-firming effects. Furthermore, the double-layer essential oil obtained by the preparation method of the present invention is more easily absorbed by the skin.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a double-layer essence oil with anti-oxidation and firming effects and a preparation method thereof. Background Art

[0002] There are many factors that cause skin wrinkling and aging, such as environmental pollution, environmental factors, life and work pressure, aging, etc. Among them, environmental factors such as ultraviolet radiation, long-term exposure of the skin to ultraviolet radiation causes photoaging, and photoaging of the skin is due to the increase of free radicals in the skin, which damages the biological membrane, promotes the cross-linking and polymerization of elastic fibers, and accelerates skin aging. There are many types of skin care products for anti-aging on the market. Some of them add vitamin-based antioxidants such as vitamin E, but these antioxidants have poor stability and their application is limited. In recent years, studies have found that some plants or Chinese herbal medicines contain antioxidant ingredients that can delay skin aging, tighten the skin, and have the effect of wrinkle removal and anti-aging. However, the anti-aging effect of existing cosmetics containing plants or Chinese herbal medicines is unstable. Therefore, based on the above problems, it is urgent to provide an essential oil product with good antioxidant and firming effects and strong stability. Summary of the Invention

[0003] The purpose of the present invention is to provide a double-layer essence oil with antioxidant and firming effects and a preparation method thereof. The product active substance adopts natural plant extracts, which is highly safe. The double-layer essence oil obtained by the preparation method of the present invention has better antioxidant and skin firming effects.

[0004] In order to achieve the above object, the present invention adopts the following technical solutions:

[0005] In a first aspect, the present invention provides an antioxidant firming composition comprising the following components: Camellia oleifera seed oil, Echium plantain seed oil, hops extract, and Scutellaria baicalensis extract.

[0006] Preferably, the antioxidant firming composition is composed of the following components in parts by weight:

[0007] 3-6 parts of deep sea camellia seed oil;

[0008] 3-6 parts of Echium plantago seed oil;

[0009] 3-6 parts of hops extract;

[0010] 3-6 parts of Radix Angelicae Dahuricae extract.

[0011] Furthermore, the antioxidant firming composition is composed of the following components in parts by weight:

[0012] 3-4 parts of deep sea camellia seed oil;

[0013] 3-4 parts of plantain leaf echium seed oil;

[0014] 3-4 parts of hops extract;

[0015] 3-4 parts of Radix Angelicae Dahuricae extract.

[0016] Optimally, the antioxidant firming composition consists of the following components in parts by weight:

[0017] 4 parts of deep sea camellia seed oil;

[0018] 4 parts of Echium seed oil;

[0019] 4 parts of hops extract;

[0020] 4 parts of Radix Angelicae Dahuricae Extract.

[0021] In a second aspect, the present invention provides a double-layer essence oil, which is composed of the following components in percentage by weight: 1-5wt% of an antioxidant firming composition, 35-40wt% of isohexadecane, 2-4wt% of C13-16 isoparaffin, 0.1-0.3wt% of caprylic / capric triglyceride, 1-5wt% of coconut oil caprylate / caprate, 3-6wt% of butylene glycol, 0.1-0.5wt% of 1,2-hexanediol, 0.1-0.3wt% of parahydroxyacetophenone, 0.1-0.5wt% of potassium methoxysalicylate, and the balance being deionized water.

[0022] Preferably, the double-layer essential oil is composed of the following components in percentage by mass:

[0023] 1-3 wt% of antioxidant firming composition;

[0024] Isohexadecane 35-38 wt%;

[0025] C13-16 isoparaffin 2-3 wt%;

[0026] Caprylic / capric triglyceride 0.1-0.2 wt%;

[0027] Coconut oil alcohol-caprylate / caprate 1-3wt%;

[0028] Butanediol 3-4 wt%;

[0029] 1,2-Hexanediol 0.1-0.3wt%;

[0030] 4-Hydroxyacetophenone 0.1-0.2 wt%;

[0031] Potassium methoxysalicylate 0.1-0.3wt%;

[0032] The balance is deionized water.

[0033] Optimally, the double-layer essence oil is composed of the following components in percentage by mass:

[0034] 3 wt% of antioxidant firming composition;

[0035] Isohexadecane 38 wt%;

[0036] C13-16 isoparaffin 3 wt%;

[0037] Caprylic / capric triglyceride 0.2 wt%;

[0038] Coco-caprylate / caprate 3wt%;

[0039] Butanediol 4wt%;

[0040] 1,2-Hexanediol 0.3 wt%;

[0041] 4-Hydroxyacetophenone 0.2 wt%;

[0042] Potassium methoxysalicylate 0.3wt%;

[0043] The balance is deionized water.

[0044] In a third aspect, the present invention provides a method for preparing a double-layer essential oil, the specific steps of which are as follows:

[0045] S1. Place Camellia oleifera seed oil, Echium plantain seed oil, coconut oil caprylate / caprate, isohexadecane, C13-16 isoparaffin, and caprylic / capric triglyceride in an emulsifier, heat to 50-55°C, and mix well to obtain phase A;

[0046] S2. Place butanediol, 1,2-hexanediol, p-hydroxyacetophenone, and deionized water in an emulsifier, heat to 75-80°C, mix well, cool to 45-48°C, add potassium methoxysalicylate, hops extract, and yellow hop extract, and mix well to obtain phase B;

[0047] S3. Mix phase A and phase B, sterilize, fill, and seal for storage.

[0048] Compared with the prior art, the present invention has the following beneficial effects: the antioxidant firming composition of the present invention comprises deep-sea camellia seed oil, echium plantaginis seed oil, hops extract, and yellow caltrop extract, wherein deep-sea camellia seed oil and yellow caltrop extract provide vitamin E and flavonoids, capture free radicals, and reduce oxidative stress; echium plantaginis seed oil and hops extract convert ALA and SDA into EPA and α-acid and β-acid, thereby enhancing the overall antioxidant effect; echium plantaginis seed oil indirectly improves the nutrition and oxygen supply of the skin by promoting cardiovascular health, and Deep-sea camellia seed oil directly forms a protective film on the skin surface. The synergistic effect of the two can effectively enhance the firmness and elasticity of the skin; the anti-inflammatory and repairing effects of the yellow celery extract, combined with the antioxidant capacity of the hops extract, can reduce inflammatory responses and ultraviolet damage to the skin, and promote skin health; the present invention pioneered the use of deep-sea camellia seed oil, echium seed oil, hops extract, and yellow celery extract together. After experiments, it was found that the above four ingredients used together have significant synergistic effects in anti-oxidation and skin firming, and have excellent production and application value. DETAILED DESCRIPTION

[0049] In order to better understand the present invention, the present invention is further described below in conjunction with specific examples. The terms used in the examples are for describing specific embodiments and do not constitute a limitation on the scope of protection of the present invention.

[0050] For the following tests where specific conditions are not specified, the experimental methods are generally based on conventional conditions or those recommended by the manufacturer. Unless otherwise specified, percentages and parts are calculated by weight.

[0051] Some of the raw materials and their sources are as follows:

[0052] Camellia purse seed oil: purchased from Guangzhou Chenyu Biotechnology Co., Ltd.

[0053] Echium plantain seed oil: purchased from Guangzhou Artemisia Biotechnology Co., Ltd.

[0054] Hops extract: purchased from Shaanxi Hengling Natural Biological Products Co., Ltd.

[0055] Huang Xixin extract: purchased from Shaanxi Yinuo Biotechnology Co., Ltd.;

[0056] The mass fractions of the components in the antioxidant firming compositions of the examples and comparative examples of the present invention are shown in Table 1 below:

[0057] Table 1 Mass fractions of each component in the antioxidant firming composition

[0058]

[0059] Note: “” means no addition, the missing mass parts shall be replaced by equal amount of deionized water.

[0060] A double-layer essential oil, the mass percentages of its components are shown in Table 2 below.

[0061] Table 2 Double-layer essential oil mass percentage

[0062]

[0063]

[0064]

[0065]

[0066] The preparation methods of the above-mentioned essential oils 1, 4, 5, essential oils ①-⑧, and essential oil blanks are as follows:

[0067] S1. Place Camellia sinensis seed oil, Echium plantain seed oil, coconut oil caprylate / caprate, isohexadecane, C13-16 isoparaffin, and caprylic / capric triglyceride in an emulsifier, heat to 53°C, and mix thoroughly to obtain phase A.

[0068] S2. Place butanediol, 1,2-hexanediol, p-hydroxyacetophenone, and deionized water in an emulsifier, heat to 80°C, mix well, cool to 47°C, add potassium methoxysalicylate, hops extract, and yellow hop extract, and mix well to obtain phase B;

[0069] S3. Mix phase A and phase B, sterilize, fill, and seal for storage.

[0070] The preparation method of essential oil 2 is as follows:

[0071] S1. Place Camellia sinensis seed oil, Echium plantain seed oil, coconut oil caprylate / caprate, isohexadecane, C13-16 isoparaffin, and caprylic / capric triglyceride in an emulsifier, heat to 50°C, and mix well to obtain phase A.

[0072] S2. Place butanediol, 1,2-hexanediol, p-hydroxyacetophenone, and deionized water in an emulsifier, heat to 80°C, mix well, cool to 45°C, add potassium methoxysalicylate, hops extract, and yellow hop extract, and mix well to obtain phase B;

[0073] S3. Mix phase A and phase B, sterilize, fill, and seal for storage.

[0074] The preparation method of essential oil 3 is as follows:

[0075] S1. Place Camellia oleifera seed oil, Echium plantain seed oil, coconut oil caprylate / caprate, isohexadecane, C13-16 isoparaffin, and caprylic / capric triglyceride in an emulsifier, heat to 55°C, and mix well to obtain phase A.

[0076] S2. Place butanediol, 1,2-hexanediol, p-hydroxyacetophenone, and deionized water in an emulsifier, heat to 75°C, mix well, cool to 48°C, add potassium methoxysalicylate, hops extract, and yellow angelica extract, and mix well to obtain phase B;

[0077] S3. Mix phase A and phase B, sterilize, fill, and seal for storage.

[0078] Essential Oil ⑨:

[0079] The formula of Essence Oil 9 is the same as that of Essence Oil 1. However, the preparation method is as follows:

[0080] Potassium methoxysalicylate, hops extract, yellow calendula extract, deep-sea camellia seed oil, plantain echium seed oil, coconut oil caprylate / caprate, isohexadecane, C13-16 isoparaffin, caprylic / capric triglyceride, butylene glycol, 1,2-hexanediol, p-hydroxyacetophenone, and deionized water are placed in an emulsifying pot, first heated to 55°C and mixed evenly, then heated to 80°C and mixed evenly, cooled to 47°C, mixed and stirred evenly, sterilized, filled, and sealed for storage.

[0081] Essential Oil ⑩:

[0082] Essence Oil ⑩ differs from Essence Oil 1 in that it contains only Phase A.

[0083] essential oils

[0084] essential oils Unlike Essence Oil 1, it only contains Phase B.

[0085] Experiment 1: Human safety test

[0086] 1-1. Human skin patch test

[0087] Tested substances: Essential oils 1-5 and essential oil

[0088] Subject selection: Healthy volunteers aged 21-57 years old were selected. They voluntarily participated and signed the informed consent form and were able to complete the trial as required during the test period. Each group had 30 subjects.

[0089] Experimental method: Select qualified patch equipment and adopt closed patch test method. Pipette 0.020mL-0.025mL (liquid) of the test substance and place it in the patch tester. Apply it to the subject's back or flexor side of the forearm with hypoallergenic tape. Remove the test substance after 24 hours. Observe and record the skin reaction 0.5, 24, and 48 hours after removal.

[0090] Result analysis: The grading of adverse skin reactions was determined according to the grading of adverse skin reactions in human trials specified in the "Technical Specifications for Safety of Cosmetics" (2015 edition) issued by the State Food and Drug Administration. The grading standards are shown in Table 1-1, and the results are shown in Table 1-2.

[0091] Table 1-1 Grading standards for adverse skin reactions

[0092]

[0093]

[0094] Table 1-2 Human skin patch test results

[0095]

[0096]

[0097] 1-2. Human testing on sensitive skin

[0098] Tested substances: Essential oils 1-5 and essential oils

[0099] Subject selection: Healthy volunteers aged 21-44 years old, who meet the requirements of lactic acid sting test, voluntarily participate and sign informed consent, and can complete the test as required during the test period are selected. Each group has 30 subjects.

[0100] Experimental Method: Volunteers randomly divided their faces into two groups: a test side and a control side. After cleansing, the product was evenly applied to the test side according to the product instructions. The control side remained untreated. After a 28-day trial, volunteers reported any local skin reactions. Any adverse reactions were evaluated by a dermatologist.

[0101] Result analysis: The grading of adverse skin reactions was determined according to the grading of adverse skin reactions in human trials specified in the "Technical Specifications for Safety of Cosmetics" (2015 edition) issued by the State Food and Drug Administration. The grading standards are shown in Table 1-1, and the results are shown in Table 1-3.

[0102] Table 1-3 Results of human trial tests

[0103]

[0104] Combined with the results of human skin patch test and sensitive skin test, during the test period, the essence oil 1-5 and essence oil No subjects experienced adverse skin reactions, indicating that essential oils 1-5 and essential oil It has good safety, is mild and non-irritating, and is suitable for people with sensitive skin.

[0105] Test 2: Antioxidant test

[0106] Sample solution: The sample solution is divided into a sample group and a positive control group; wherein the sample group is composed of essence oil 1-5, essence oil The blank essential oil was prepared into aqueous solution with a concentration of 5 mg / mL, the positive control group was a Vc aqueous solution with a concentration of 1 mg / L, and the solvent used in the sample solution was sterile deionized water.

[0107] Note: Before use, the sample group should be emulsified using an AES3300 fully automatic multi-channel emulsifier (Nanjing Baode Biotechnology Co., Ltd.). The test should be completed within 60 minutes after the sample is emulsified.

[0108] Test steps:

[0109] 1. Take 2mL of sample solution and 2mL of 2*10 -4 Mix 100 mol / L DPPH anhydrous ethanol solution (A1);

[0110] 2. Take 2mL of sterile deionized water and 2mL of 2*10 -4 Mix 100 mol / L DPPH anhydrous ethanol solution (A2);

[0111] 3. Take 2 mL of anhydrous ethanol and mix it with the sample solution (A3);

[0112] 4. After A1, A2, and A3 have reacted for 40 minutes, measure the absorbance of A1, A2, and A3 at 517 nm.

[0113] The formula for calculating the clearance rate is: Clearance rate (%) = [1-(A1-A3) / A2]*100%

[0114] The experimental results are shown in Table 4.

[0115] Test 3: Firmness test determination

[0116] Sample solution: The sample solution was divided into a sample group and a positive control group; the sample group consisted of essence oil 1-5, essence oil and blank essential oil, which were prepared into a 0.5 mg / L aqueous solution using sterile deionized water; the positive control group was TGF-β1, with a concentration of 100 ng / mL.

[0117] Test method: Refer to T / SHRH 031-2020 "Test of cosmetic firming and anti-wrinkle efficacy - Determination of type I collagen content in fibroblasts in vitro" to test essence oils 1-5, essence oil The effects of the essential oil and blank essential oil on promoting the synthesis of type I collagen were tested at a concentration of 0.5 mg / L. The test results are shown in Table 4.

[0118] Table 4 Antioxidant and firming effects

[0119]

[0120]

[0121] Note: *P<0.05.

[0122] Comparing essence oils 1-5, the results show that essence oil 3 has the best antioxidant effect and the best effect in increasing type I collagen. However, compared with the addition amount of essence oil 3, essence oil 1 has the best cost-effectiveness, so essence oil 1 is selected as the best embodiment.

[0123] Comparison of essential oil 1 with essential oils ①-⑧ shows that the four compositions provided by the present invention have significant synergistic effects. The results show that the preparation method defined in the present invention can effectively enhance the antioxidant effect of the essential oil and the effect of synthesizing type I collagen; Comparison of essential oil 1 and essential oil It can be seen that compared with using phases AB separately, using them together has better antioxidant effects and the effect of promoting the synthesis of type I collagen.

[0124] The embodiments described above are part of the embodiments of the present application, rather than all of the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the present application for protection, but merely represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without making creative efforts are within the scope of protection of the present application.

Claims

1. A double-layer essential oil, characterized in that: The double-layer essence oil is composed of the following components in percentage by mass: 1-5 wt% of antioxidant firming composition; Isohexadecane 35-40 wt%; C13-16 isoparaffin 2-4 wt%; Caprylic / capric triglyceride 0.1-0.3 wt%; Coconut oil alcohol-caprylate / caprate 1-5wt%; Butanediol 3-6 wt%; 1,2-Hexanediol 0.1-0.5wt%; 4-Hydroxyacetophenone 0.1-0.3 wt%; Potassium methoxysalicylate 0.1-0.5wt%; The balance is deionized water; The antioxidant firming composition is composed of the following components in parts by mass 3-6 parts of deep sea camellia seed oil; 3-6 parts of Echium plantago seed oil; 3-6 parts of hops extract; 3-6 parts of Radix Angelicae Dahuricae extract.

2. The double-layer essential oil according to claim 1, characterized in that The antioxidant firming composition is composed of the following components in parts by weight: 3-4 parts of deep sea camellia seed oil; 3-4 parts of plantain leaf echium seed oil; 3-4 parts of hops extract; 3-4 parts of Radix Angelicae Dahuricae extract.

3. The double-layer essential oil according to claim 1, characterized in that The antioxidant firming composition is composed of the following components in parts by weight: 4 parts of deep sea camellia seed oil; 4 parts of Echium seed oil; 4 parts of hops extract; 4 parts of Radix Angelicae Dahuricae Extract.

4. The double-layer essential oil according to claim 1, characterized in that The double-layer essence oil is composed of the following components in percentage by mass: 1-3 wt% of antioxidant firming composition; Isohexadecane 35-38 wt%; C13-16 isoparaffin 2-3 wt%; Caprylic / capric triglyceride 0.1-0.2 wt%; Coconut oil alcohol-caprylate / caprate 1-3wt%; Butanediol 3-4 wt%; 1,2-Hexanediol 0.1-0.3wt%; 4-Hydroxyacetophenone 0.1-0.2 wt%; Potassium methoxysalicylate 0.1-0.3wt%; The balance is deionized water.

5. The double-layer essential oil according to claim 1, characterized in that The double-layer essence oil is composed of the following components in percentage by mass: 3 wt% of antioxidant firming composition; Isohexadecane 38 wt%; C13-16 isoparaffin 3 wt%; Caprylic / capric triglyceride 0.2 wt%; Coco-caprylate / caprate 3wt%; Butanediol 4wt%; 1,2-Hexanediol 0.3 wt%; 4-Hydroxyacetophenone 0.2 wt%; Potassium methoxysalicylate 0.3wt%; The balance is deionized water.

6. The double-layer essence oil according to any one of claims 1 to 5, characterized in that The preparation method of the double-layer essential oil comprises the following steps: S1. Place Camellia oleifera seed oil, Echium plantain seed oil, coconut oil caprylate / caprate, isohexadecane, C13-16 isoparaffin, and caprylic / capric triglyceride in an emulsifier, heat to 50-55°C, and mix well to obtain phase A; S2. Place butanediol, 1,2-hexanediol, p-hydroxyacetophenone, and deionized water in an emulsifier, heat to 75-80°C, mix well, cool to 45-48°C, add potassium methoxysalicylate, hops extract, and yellow hop extract, and mix well to obtain phase B; S3. Mix phase A and phase B, sterilize, fill, and seal for storage.

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