Bacterial fermentation liquid separation and extraction device and separation and extraction method thereof

Abstract

The invention discloses a bacterial fermentation liquid separation and extraction device and a separation and extraction method. The bacterial fermentation liquid separation and extraction device comprises a separation tank body, a primary separation mechanism and a secondary separation mechanism, wherein the separation tank body is provided with a separation cavity; the primary separation mechanism comprises a filtering part, a stirring part, a weight detection part and a pressure detection part, the filtering part is provided with filtering holes and arranged in the separation cavity, the stirring part is connected to the separation tank body, the weight detection part is connected to the separation tank body, and the pressure detection part is connected to the separation tank body; the secondary separation mechanism comprises a separation cylinder and a rotating cylinder, the separation cylinder is communicated with the separation cavity, the rotating cylinder is provided with a plurality of separation holes, and the rotating cylinder is rotatably arranged in the separation cylinder. According to the bacterial fermentation liquor separation and extraction device, thalli can be efficiently extracted and recycled, solid metabolites can be rapidly separated, the recycled thalli can be reused, and the production cost is low.

Description

Technical Field

[0001] The present application relates to the field of bioengineering and fermentation liquid separation technology, and in particular to a bacterial fermentation liquid separation and extraction device and a separation and extraction method thereof. Background Art

[0002] Fermentation is the process of preparing microbial cells, direct metabolites or secondary metabolites by means of the life activities of microorganisms under aerobic or anaerobic conditions. Fermentation is widely used in the food industry, biological and chemical industries. At present, fermentation is the process of producing and accumulating a large amount of metabolites required for fermentation by large-scale growth and cultivation of microorganisms or animal cells and plant cells to cause chemical and physiological changes.

[0003] In the process of processing bacterial fermentation broth, for high-value bacteria, it is necessary to recover the high-value bacteria mixed into the fermentation product and separate them from the solid components in the fermentation broth. The traditional technology of separating bacteria from fermentation broth generally adopts filtration, centrifugal separation or precipitation methods. Among them, the filtration and precipitation separation methods are due to the small diameter of bacteria. During the separation process, the solids in the fermentation broth can easily block the filter screen or filter membrane, resulting in a long separation time and unsatisfactory separation effect; in the centrifugal separation method, when the density of the bacteria is not much different from the density of the solids in the fermentation broth, the separation effect is poor. Summary of the invention

[0004] Based on this, it is necessary to provide a bacterial fermentation liquid separation and extraction device.

[0005] An embodiment of the present application provides a bacterial fermentation liquid separation and extraction device.

[0006] A bacterial fermentation liquid separation and extraction device comprises a separation tank body, a primary separation mechanism and a secondary separation mechanism, wherein the separation tank body has a closable separation chamber;

[0007] The primary separation mechanism includes a filtering component, a stirring component, a weight detection component and a pressure detection component. The filtering component is arranged in the separation chamber to perform primary separation on the bacterial fermentation liquid to obtain bacterial bodies and concentrated fermentation liquid. The stirring component is connected to the separation tank body to stir the bacterial fermentation liquid in the separation chamber. The weight detection component is used to detect the weight m of the bacterial fermentation liquid in the separation tank body. The pressure detection component is used to detect the pressure in the separation chamber.

[0008] The secondary separation mechanism includes a separation cylinder and a rotating cylinder, the rotating cylinder is connected to the separation chamber to collect the concentrated fermentation liquid after the primary separation, the rotating cylinder has a plurality of separation holes, the aperture of the separation holes is larger than the diameter of the bacteria and smaller than the diameter of the solid metabolites, the rotating cylinder can be rotatably arranged in the separation cylinder, and the rotating cylinder can be rotated to achieve secondary separation of the concentrated fermentation liquid to obtain solid metabolites and separation liquid containing bacteria.

[0009] The bacterial fermentation liquid separation and extraction device of the present invention can realize the efficient extraction and recovery of bacterial bodies and the rapid separation of solid metabolites. The recovered bacterial bodies can be reused, which is conducive to reducing production costs.

[0010] In some embodiments, the separation tank body also has a liquid inlet, a liquid outlet and a pipette port connected to the separation chamber, the position of the liquid outlet along the height direction of the separation tank body is higher than the position of the liquid inlet, the filter component is connected to the liquid outlet, and the separation cylinder is connected to the separation chamber through the pipette port.

[0011] In some embodiments, the filtering component includes a filter element and a liquid collecting pipe arranged in the separation chamber, the filter element has a plurality of filter holes, the pore size of the filter holes is larger than the diameter of the bacteria and smaller than the diameter of the solid metabolic products, the filter element is connected to the liquid collecting pipe and can pass through the liquid collecting pipe.

[0012] In some embodiments, there are multiple filter elements, and the multiple filter elements are distributed at intervals in the separation chamber.

[0013] In some embodiments, the filter element extends from the top of the separation tank body to the bottom of the separation tank body, and there is a gap between the filter element and the bottom of the separation tank body.

[0014] In some embodiments, the liquid collecting pipe is close to the top of the separation tank.

[0015] In some embodiments, the separation tank body further has a backwash port, which is communicated with the liquid collecting pipe, and is used for externally connecting backwash liquid to backwash the clogged filter element.

[0016] In some embodiments, the stirring component includes a stirring shaft, a seal, a stirring paddle and a stirring drive, a portion of the stirring shaft extends into the separation chamber and another portion extends to the outside of the separation tank body, the stirring shaft is dynamically sealed with the separation tank through the seal, the stirring paddle is located in the separation chamber and connected to the stirring shaft, and the stirring drive is installed on the separation tank body and connected to the stirring shaft.

[0017] In some embodiments, the stirring paddle is disposed below the filtering component and close to the bottom of the separation chamber.

[0018] In some embodiments, the stirring component further includes a driver support, the driver support is connected to the top of the separation tank, and the stirring driver is installed on the driver support.

[0019] In some embodiments, the stirring component further includes a coupling, and the stirring drive is connected to the stirring shaft via the coupling.

[0020] In some embodiments, a support is connected to the bottom of the separation tank body, the separation tank body is mounted on a plurality of the support members, and the weight detection component is mounted on the support member and connected to the separation tank body.

[0021] In some of the embodiments, a pneumatic valve is provided on the connecting pipeline between the separation cylinder and the separation tank body.

[0022] In some embodiments, the connecting pipeline between the separation cylinder and the separation tank body is arranged in a vertical direction, the separation cylinder and the rotating cylinder are both arranged obliquely, and the rotating axis of the rotating cylinder has an angle with the vertical direction.

[0023] In some embodiments, the secondary separation mechanism further includes a liquid return pipeline, which is connected to the separation cylinder to drain the separation liquid containing the bacteria after the secondary separation.

[0024] In some embodiments, the secondary separation mechanism further includes a rotation driving component, and the rotation driving component is connected to the rotating drum to drive the rotating drum to rotate.

[0025] In some of the embodiments, the bacterial fermentation liquid separation and extraction device further includes a control mechanism, and the control mechanism is electrically connected to the stirring component, the weight detection component and the pressure detection component.

[0026] In some embodiments, the bacterial fermentation liquid separation and extraction device further comprises an alarm mechanism, which is signal-connected to the pressure detection component. When the pressure value in the separation chamber detected by the pressure detection component is greater than a preset pressure value, the alarm mechanism operates.

[0027] An embodiment of the present application also provides a method for separating and extracting bacterial fermentation broth.

[0028] A method for separating and extracting bacterial fermentation liquid, using the above-mentioned bacterial fermentation liquid separation and extraction device, comprises the following steps:

[0029] Placing the bacterial fermentation liquid in the separation chamber of the separation tank, controlling the weight detection component to detect the weight m of the bacterial fermentation liquid in the separation tank in real time, and controlling the pressure detection component to detect the pressure value P in the separation chamber in real time;

[0030] Controlling the stirring component to stir the bacterial fermentation liquid in the separation chamber, so that the bacterial fermentation liquid forms a filter cake layer on the surface of the filter element of the filter component, and the filtrate containing the bacteria passes through the filter cake layer and is discharged from the separation tank after the primary separation of the filter component;

[0031] Determine whether the primary separation is completed according to the pressure value P and the liquid density ρ in the separation chamber calculated from the weight m of the bacterial fermentation liquid in the separation tank body;

[0032] When it is determined that the primary separation is completed, the concentrated fermentation liquid in the separation chamber is controlled to enter the rotating drum, and the rotating drum is controlled to rotate to achieve secondary separation of the concentrated fermentation liquid to obtain solid metabolites and separation liquid containing bacteria.

[0033] In some embodiments, when the pressure value P ≥ the preset pressure value P1, it indicates that the filter element of the filter component is clogged, and backwash liquid is input through the backwash port on the separation tank body to backwash the filter element of the filter component until the pressure value P < the preset pressure value P2.

[0034] In some embodiments, when the pressure value P≥the preset pressure value P1, it indicates that the filter element of the filter component is clogged, and the control alarm mechanism is operated.

[0035] In some embodiments, the return liquid pipeline is controlled to drain the separation liquid containing bacterial cells after the secondary separation and merge it with the filtrate containing bacterial cells after the primary separation.

[0036] In some embodiments, the thickness of the filter cake layer is controlled to be 0.4 mm to 1.6 mm.

[0037] In some embodiments, when judging whether the primary separation is completed, the following steps are included: calculating the liquid density ρ in the separation chamber according to the weight m of the bacterial fermentation liquid in the separation tank body, judging the relationship between the liquid density ρ and the critical density ρ0, when ρ≥ρ0 and the pressure value P<the preset pressure value P0, it indicates that the primary separation is completed.

[0038] The above-mentioned bacterial fermentation liquid separation and extraction device can solve the problem that when the density difference between the bacteria and solid metabolites in the fermentation liquid is very small in the traditional technology, it is difficult to separate them by using the centrifugal separation method, and the filter screen or filter membrane of the filter component is easily blocked by using the conventional filtration or precipitation method. The present application sets up a closed separation environment and strictly controls the thickness of the solid layer such as the filter cake layer in the separation process to ensure that the activity of most of the bacteria recovered by the primary separation is not affected. It can meet the strict requirements of the bacteria for the living environment and the conditions where it is extremely difficult to survive in the unsterilized air environment and solid precipitation. It can achieve efficient extraction and recovery of bacteria and rapid separation of solid metabolites. The recovered bacteria can be reused, which can greatly reduce production costs.

[0039] The bacterial fermentation liquid separation and extraction device of the present invention increases stirring control during the separation process, realizes a thinner filter cake layer on the filter element of the filter component, controls the thickness of the filter cake layer to be 0.4mm to 1.6mm, completes the filtration and separation process under a very thin filter cake layer, can recover most of the bacteria in the primary separation, reduces production costs, and ensures that the activity of the bacteria is not destroyed. Furthermore, the present application performs secondary separation on the solid metabolites contained in the concentrated fermentation liquid after the primary separation through a secondary separation mechanism, directly obtains the solid metabolites generated by fermentation, reduces the time for the fermentation liquid to be transferred between devices, and improves the separation and extraction efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present application, and those skilled in the art can obtain other drawings based on these drawings without creative work.

[0041] In order to more completely understand the present application and its beneficial effects, the following description will be given in conjunction with the accompanying drawings. In the following description, the same reference numerals represent the same parts.

[0042] FIG1 is a schematic diagram of a bacterial fermentation liquid separation and extraction device according to an embodiment of the present invention;

[0043] FIG. 2 is a schematic cross-sectional view of a bacterial fermentation liquid separation and extraction device according to an embodiment of the present invention.

[0044] Description of Reference Numerals

[0045] 10. Bacterial fermentation liquid separation and extraction device; 100. Separation tank; 101. Separation chamber; 102. Liquid inlet; 103. Liquid outlet; 104. Transfer port; 105. Backwash port; 106. Baffle; 107. Support; 108. Connecting cylinder; 109. Cover plate; 200. Primary separation mechanism; 210. Filter component; 211. Filter element; 212. Liquid collecting pipe; 220. Stirring component; 221. Stirring shaft; 222. Seal; 223. Stirring paddle; 224. Stirring drive; 225. Drive support; 226. Coupling; 230. Weight detection component; 240. Pressure detection component; 300. Secondary separation mechanism; 310. Separation cylinder; 320. Rotating cylinder; 330. Liquid return line; 340. Rotating drive component; 350. Pneumatic valve. DETAILED DESCRIPTION

[0046] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below in conjunction with the accompanying drawings. In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present invention, so the present invention is not limited by the specific embodiments disclosed below.

[0047] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0048] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0049] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0050] In the description of the present invention, "several" means more than one, "many" means more than two, "greater than", "less than", "exceed", etc. are understood to exclude the number itself, and "above", "below", "within", etc. are understood to include the number itself. If there is a description of "first" or "second", it is only used for the purpose of distinguishing the technical features, and cannot be understood as indicating or implying the relative importance or implicitly indicating the number of the indicated technical features or implicitly indicating the order of the indicated technical features.

[0051] In this article, "optionally", "optional", "optional" means optional, that is, it means to be selected from any of the two parallel solutions of "yes" or "no". If there are multiple "optional" in a technical solution, unless otherwise specified and there is no contradiction or mutual restriction, each "optional" is independent. In this application, the descriptions such as "optionally contain" and "optionally include" mean "contain or not contain".

[0052] In the present application, when it comes to a numerical interval (i.e., a numerical range), unless otherwise specified, the distribution of the optional numerical values ​​in the numerical interval is considered to be continuous, and includes the two numerical endpoints (i.e., the minimum and maximum values) of the numerical interval, and each numerical value between the two numerical endpoints. Unless otherwise specified, when the numerical interval only refers to an integer in the numerical interval, it includes the two endpoint integers of the numerical range, and each integer between the two endpoints, which is equivalent to directly listing each integer. When multiple numerical ranges are provided to describe features or characteristics, these numerical ranges can be merged. In other words, unless otherwise specified, the numerical range disclosed in the present application should be understood to include any and all sub-ranges included therein. The "numerical value" in the numerical interval can be any quantitative value, such as a number, a percentage, a ratio, etc. "Numerical interval" allows for broadly including quantitative intervals such as percentage intervals, ratio intervals, and ratio intervals.

[0053] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those generally understood by those skilled in the art of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0054] The present application provides a bacterial fermentation liquid separation and extraction device 10 to solve at least one of the following problems in the prior art bacterial fermentation liquid separation method: (1) When using the filtration and precipitation method, due to the small diameter of the bacteria, the solid metabolites in the fermentation liquid are easy to block the filter screen or filter membrane during the separation process, resulting in a long separation time and unsatisfactory separation effect; (2) When using the centrifugal separation method, when the density of the bacteria is not much different from the solid density in the fermentation liquid, the separation effect is poor. The bacterial fermentation liquid separation and extraction device 10 will be described below in conjunction with the accompanying drawings.

[0055] The bacterial fermentation liquid separation and extraction device 10 of the present application can be used for the purpose of separating bacteria from bacterial fermentation liquid. The separation and extraction method of the present invention reduces the production cost by reusing the recovered bacteria, and directly obtains solid metabolites by completing two-stage separation in one device, thereby improving the separation efficiency. The bacterial fermentation liquid separation and extraction device 10 and the separation and extraction method provided in the embodiment of the present application can be applied to the separation and extraction of bacterial fermentation liquid in which the density difference between bacteria and solid metabolites is very small, and efficiently completes the recovery of bacteria and the separation of solid metabolites. It should be noted that the diameter of the bacteria to be separated in the present application is smaller than the diameter of the solid metabolites.

[0056] In order to more clearly illustrate the structure of the bacterial fermentation liquid separation and extraction device 10, the bacterial fermentation liquid separation and extraction device 10 will be introduced below in conjunction with the accompanying drawings. For example, please refer to Figure 1, which is a structural schematic diagram of the bacterial fermentation liquid separation and extraction device 10 provided in an embodiment of the present application.

[0057] For example, please refer to Fig. 1, a bacterial fermentation liquid separation and extraction device 10 includes a separation tank 100, a primary separation mechanism 200 and a secondary separation mechanism 300. The separation tank 100 has a separation chamber 101 that can be sealed.

[0058] Please refer to FIG. 1 and FIG. 2 , FIG. 2 is a schematic diagram of the cross-sectional structure of a bacterial fermentation liquid separation and extraction device 10 according to an embodiment of the present invention, wherein the primary separation mechanism 200 includes a filter component 210, a stirring component 220, a weight detection component 230 and a pressure detection component 240. The filter component 210 is disposed in the separation chamber 101 to perform primary separation on the bacterial fermentation liquid to obtain bacterial cells and concentrate the fermentation liquid. The stirring component 220 is connected to the separation tank body 100 to stir the bacterial fermentation liquid in the separation chamber 101. The weight detection component 230 is used to detect the weight m of the bacterial fermentation liquid in the separation tank body 100, and the weight detection component 230 can be connected to the separation tank body 100. The pressure detection component 240 is used to detect the pressure in the separation chamber 101 and obtain a real-time pressure value P, and the pressure value P is used to determine whether the filter element in the separation chamber is blocked and whether the primary separation is completed, and the pressure detection component 240 can be connected to the separation tank body 100.

[0059] Please refer to FIG. 1 and FIG. 2 , the secondary separation mechanism 300 includes a separation cylinder 310 and a rotating cylinder 320. The rotating cylinder 320 is connected to the separation chamber 101. The rotating cylinder 320 has a plurality of separation holes, and the aperture of the separation hole is larger than the diameter of the bacterial body and smaller than the diameter of the solid metabolite. The rotating cylinder 320 is rotatably arranged in the separation cylinder 310. The rotating cylinder 320 can be rotated to achieve secondary separation of the concentrated fermentation liquid to obtain solid metabolites and a separation liquid containing bacterial bodies. By rotating the rotating cylinder 320, the concentrated fermentation liquid in the rotating cylinder 320 can be centrifuged, and solid metabolites and a separation liquid containing bacterial bodies can be obtained. The separated separation liquid containing bacterial bodies can continue to flow back to the separation chamber for primary separation, or directly merged into the filtrate containing bacterial bodies after the primary separation, and the recovered bacterial bodies can be reused.

[0060] The above-mentioned bacterial fermentation liquid separation and extraction device 10 can solve the problem that when the density difference between the bacteria and solid metabolites in the fermentation liquid is very small, it is difficult to separate them by using the centrifugal separation method in the traditional technology, and the filter screen or filter membrane of the filter component 210 is easily blocked by using the conventional filtration or precipitation method. The present application sets up a closed separation environment and strictly controls the thickness of the solid layer such as the filter cake layer during the separation process to ensure that the activity of most of the recovered bacteria is not affected, and can meet the strict requirements of the bacteria for the living environment and the conditions where it is extremely difficult to survive in the non-sterile air environment and solid precipitation. Efficient extraction and recovery of bacteria and rapid separation of solid metabolites are achieved, and the recovered bacteria can be reused, which can greatly reduce production costs.

[0061] In some embodiments, as shown in FIG. 2 , the separation tank body 100 further has a liquid inlet 102, a liquid outlet 103 and a pipetting port 104 communicating with the separation chamber 101. The position of the liquid outlet 103 is higher than the position of the liquid inlet 102 along the height direction of the separation tank body 100. The filter component 210 is connected to the liquid outlet 103 for primary separation, and the separation cylinder 310 is connected to the separation chamber 101 through the pipetting port 104.

[0062] In some embodiments, as shown in FIG. 2 , a baffle 106 is further provided in the separation tank 100. The baffle 106 is located close to the lower portion of the separation chamber 101. The baffle 106 is used to provide a certain shear dispersion effect to the fermentation liquid during the stirring process. The size of the baffle 106 should not be too large so as to improve the shear dispersion effect. Preferably, there can be a plurality of baffles 106, and the plurality of baffles 106 are arranged at intervals. The shape and size of the baffle 106 are not limited, so as to achieve turbulence and improve the shear dispersion effect.

[0063] In some embodiments, as shown in FIG. 1 and FIG. 2 , a connection tube 108 and a cover plate 109 are connected to the top of the separation tank body 100. The cover plate 109 can be detachably connected to the connection tube 108. The cover plate 109 can be opened to open the separation tank body 100, so as to realize the processes of testing, repairing, replacing, cleaning, etc. of each mechanism in the separation chamber 101.

[0064] In some of the embodiments, please refer to FIG. 2 , the filter component 210 has filter holes. The pore size of the filter holes is larger than the diameter of the bacteria and smaller than the diameter of the solid metabolites. In a specific example, the filter component 210 includes a filter element 211 and a liquid collecting pipe 212 arranged in the separation chamber 101. The filter element 211 has a plurality of filter holes, and the filter element 211 is connected to the liquid collecting pipe 212 and can pass through the liquid collecting pipe 212. It should be noted that the diameter of the bacteria generally refers to the maximum diameter of the bacteria, and the diameter of the solid metabolites generally refers to the minimum diameter of the solid metabolites.

[0065] In the present application, the pore size of the filter hole is slightly larger than the diameter of the bacteria and smaller than the diameter of the solid metabolites to improve the filtering accuracy of the filter element 211. The size of bacteria is approximately between 0.6 μm and 3.2 μm, and the diameter of solid metabolites is generally above 5 μm. Therefore, the pore size of the filter element 211 is slightly larger than 3 μm, for example, the pore size of the filter element 211 is 3.1 μm to 3.5 μm to ensure efficient filtration of bacteria. The pore size of the separation hole can be 3.1 μm to 3.5 μm.

[0066] In some embodiments, there are multiple filter elements 211. Multiple filter elements 211 are spaced apart in the separation chamber 101. Please refer to FIG2, which shows three filter elements 211. It should be noted that the example shown in FIG2 is only one example, and cannot be used to limit the actual number of filter elements 211.

[0067] In some embodiments, a plurality of filter elements 211 are evenly spaced and distributed in the separation chamber 101. The evenly distributed filter elements 211 can achieve the maximum separation of bacteria in the separation chamber 101, thereby improving the separation efficiency and separation effect.

[0068] In some embodiments, the filter element 211 extends from the top of the separation tank body 100 to the bottom of the separation tank body 100, and there is a gap between the filter element 211 and the bottom of the separation tank body 100. Preferably, the length of the filter element 211 occupies 50%-90% of the height of the separation tank body 100. Further preferably, the length of the filter element 211 occupies 70%-90% of the height of the separation tank body 100.

[0069] In some embodiments, the liquid collecting pipe 212 is close to the top of the separation tank 100. Preferably, as shown in FIG. 2 , the liquid collecting pipe 212 is arranged in a horizontal state in the separation chamber 101.

[0070] In some embodiments, as shown in FIG. 2 , the separation tank 100 further has a backwash port 105, which is in communication with the liquid collecting pipe 212, and is used to connect an external backwash liquid to backwash the clogged filter element 211. The backwash port 105 can be connected to an external backwash liquid, and a certain pressure is set by the backwash liquid to backwash the liquid collecting pipe 212 and the filter element 211, thereby avoiding or reducing the blockage of the filter element 211 and increasing the service life of the filter element 211. The backwash liquid can be a specific cleaning liquid or deionized water, etc., and the backwash liquid will not have an adverse effect on the bacteria and solid metabolites in the separation chamber 101.

[0071] In some embodiments, as shown in FIG. 2 , the stirring member 220 includes a stirring shaft 221, a sealing member 222, a stirring paddle 223, and a stirring driver 224. A portion of the stirring shaft 221 extends into the separation chamber 101 and another portion extends to the outside of the separation tank body 100. The stirring shaft 221 is dynamically sealed with the separation tank through the sealing member 222. The stirring paddle 223 is located in the separation chamber 101 and connected to the stirring shaft 221. The stirring driver 224 is installed in the separation tank body 100 and connected to the stirring shaft 221.

[0072] In some of these embodiments, the agitation drive 224 may be a agitation motor.

[0073] In some embodiments, the rotation speed of the stirring driver 224 is 200 rpm to 1000 rpm. The rotation speed of the stirring driver 224 can be set according to actual needs. The structure of the stirring paddle 223 should avoid generating strong shear force on the fermentation liquid, and the rotation speed of the stirring driver 224 should not be too high to avoid the loss of bacteria due to excessive shear force. Preferably, the rotation speed of the stirring driver 224 is 400 rpm to 800 rpm.

[0074] In some embodiments, the stirring paddle 223 is disposed below the filter component 210 in the separation chamber 101 and close to the bottom of the separation tank 100. The stirring paddle 223 is as close to the bottom surface of the separation tank 100 as possible, which can improve the liquid flow efficiency and liquid flow effect of the fermentation liquid in the separation chamber.

[0075] In some embodiments, as shown in FIG. 1 and FIG. 2 , the stirring component 220 further includes a driver support 225 . The driver support 225 is connected to the top of the separation tank 100 . The stirring driver 224 is installed on the driver support 225 .

[0076] In some embodiments, as shown in FIG2 , the stirring member 220 further includes a coupling 226. The stirring driver 224 is connected to the stirring shaft 221 via the coupling 226. The arrangement of the coupling 226 facilitates the disassembly, maintenance, cleaning, and other processes between the stirring driver 224 and the stirring shaft 221.

[0077] In some embodiments, as shown in FIG. 1 and FIG. 2 , a support 107 is connected to the bottom of the separation tank 100. The separation tank 100 is mounted on a plurality of support members 107, and the weight detection component 230 is mounted on the support members 107 and connected to the separation tank 100. Preferably, the plurality of support members 107 are detachably connected to the separation tank 100.

[0078] In some embodiments, the support member 107 may be a support leg. Preferably, referring to FIG. 1 and FIG. 2 , the support member 107 is in a long strip structure.

[0079] In some of the embodiments, a pneumatic valve 350 is provided on the connecting pipeline between the separation cylinder 310 and the separation tank body 100. The pneumatic valve 350 is a device that drives the valve to open or close by compressed air. For example, the main components of the pneumatic valve 350 include: Valve body: the main structure of the valve, usually made of metal or other corrosion-resistant materials, with a fluid channel inside. Actuator: the part that converts the pressure of compressed air into mechanical motion, which can open or close the valve through linear motion or rotational motion. Positioner: used to adjust the opening position of the valve to ensure that the valve can be opened to the specified angle accurately as needed. Air source processing unit: provides clean, dry compressed air, and can adjust the pressure. Accessories: such as limit switches, solenoid valves, etc., used to achieve more complex functions and controls.

[0080] In some embodiments, the pneumatic valve 350 can be selected from (1) pneumatic ball valve: controlling the flow of fluid by rotating a ball with a hole. (2) pneumatic butterfly valve: using a disc-shaped baffle to control the flow of fluid. (3) pneumatic stop valve: using the valve stem to move up and down to drive the valve disc to control the flow of fluid. (4) pneumatic diaphragm valve: controlling the flow of fluid by the deformation of the diaphragm.

[0081] In some embodiments, the secondary separation mechanism 300 includes a liquid return pipeline 330. The liquid return pipeline 330 is connected to the separation cylinder 310 to drain the separation liquid containing the bacterial cells after the secondary separation.

[0082] In some embodiments, the liquid return line 330 is a liquid return manifold.

[0083] In some embodiments, the secondary separation mechanism 300 includes a rotation drive component 340. The separation cylinder 310 is connected to the separation chamber 101. The rotation drive component 340 is connected to the rotating cylinder 320 to drive the rotating cylinder 320 to rotate. Optionally, the rotation speed of the rotating cylinder 320 can be 1000 rpm to 3000 rpm. Preferably, the rotation speed of the rotating cylinder 320 can be 1000 rpm to 1500 rpm.

[0084] In some embodiments, the connecting pipeline between the separation cylinder 310 and the separation tank body 100 is arranged in a vertical direction. The separation cylinder 310 and the rotating cylinder 320 are both arranged in an inclined manner, the rotation axis of the rotating cylinder 320 has an angle with the vertical direction, and the liquid return pipeline 330 is connected to the separation cylinder 310 from the bottom of the separation cylinder 310. Generally speaking, after the separation tank body 100 of the present application is installed, the separation tank body 100 is distributed in a vertical direction as a whole. In the height direction, the primary separation mechanism 200 is located at the top and the secondary separation mechanism 300 is located at the bottom. Such an arrangement facilitates the concentrated fermentation liquid after the primary separation of the primary separation mechanism 200 to enter the secondary separation mechanism 300 under the action of its own weight.

[0085] In some embodiments, as shown in FIG. 2 , the angle between the rotation axis of the rotating cylinder 320 and the vertical direction is 30° to 60°, so that the rotating cylinder 320 is in an inclined state, ensuring that the separation liquid containing the bacteria in the rotating cylinder 320 can be discharged under the action of gravity. For example, the angle between the rotation axis of the rotating cylinder 320 and the vertical direction can be 30°, 40°, 45°, 50°, 60° or other angles. The setting of the rotation axis of the rotating cylinder 320 having an angle with the vertical direction facilitates the discharge of the separation liquid after the secondary separation, which can improve work efficiency.

[0086] In some embodiments, the bacterial fermentation liquid separation and extraction device 10 further includes a control mechanism, which is electrically connected to the stirring component 220 , the weight detection component 230 , the pressure detection component 240 , and the rotation driving component 340 .

[0087] In some embodiments, the control mechanism may be a PLC programmable logic controller or a computer. The control mechanism can set certain programs according to actual needs to achieve automatic operation or shutdown of the stirring component 220, the weight detection component 230, the pressure detection component 240 and the rotation drive component 340 according to the set program.

[0088] In some embodiments, the bacterial fermentation liquid separation and extraction device 10 also includes an alarm mechanism. The alarm mechanism is connected to the pressure detection component 240 signal. When the pressure value P in the separation chamber 101 detected by the pressure detection component 240 is greater than the preset pressure value P1, the alarm mechanism works. The alarm mechanism is not shown in the accompanying drawings. The pressure detection component 240 monitors the pressure value P in the separation chamber 101 in real time. When the pressure value P is within the normal value range, it indicates that the primary separation of the separation chamber 101 is proceeding steadily. When the pressure value P changes abnormally, for example, it exceeds the preset pressure value P1, it is judged that at least part of the filter element 211 in the separation chamber 101 is blocked, and the alarm mechanism works. At this time, it is necessary to open the backwash port 105 and perform backwashing to clean the attachments on the surface of the filter element 211 until the pressure value P is within the normal value range and the alarm is released.

[0089] In some embodiments, the alarm mechanism includes an alarm light, a buzzer, a vibrator, etc. The alarm mechanism can give an alarm through vision, hearing, and touch to remind the operator.

[0090] The control mechanism analyzes the real-time monitoring pressure value P data of the pressure detection component 240, and determines whether the filter element 211 is blocked according to whether the pressure value P changes abnormally and the set preset pressure value P1; by analyzing the data of the weight detection component 230, the liquid density ρ inside the separation tank 100 is calculated in real time, the process of the first-level separation is analyzed, and compared with the set critical density ρ0. When ρ reaches the set critical density ρ0, the pneumatic valve 350 is opened, and the concentrated fermentation liquid treated by the separation tank 100 is transferred to the secondary separation mechanism 300 through the pipette port 104; by analyzing the processing process in the separation chamber 101, the rotation speed of the stirring driver 224 is adjusted in real time to ensure that the bacteria and solid metabolites in the fermentation liquid can be evenly distributed in the separation chamber 101 at each stage of the processing process; by controlling the rotating drive component 340 of the secondary separation mechanism 300, the processing time of the secondary separation mechanism 300 is controlled to complete the secondary separation.

[0091] An embodiment of the present application also provides a method for separating and extracting bacterial fermentation broth.

[0092] It should be noted that, in this article, unless otherwise specified, each reaction step can be carried out in the order described herein, or can be carried out in a non-sequential manner. For example, other steps may be included between each reaction step, and the order of the reaction steps may also be appropriately swapped. This can be determined by the technician based on conventional knowledge and experience. Preferably, the reaction method herein is carried out sequentially.

[0093] A bacterial fermentation liquid separation and extraction method, using the above-mentioned bacterial fermentation liquid separation and extraction device 10, comprises the following steps:

[0094] The bacterial fermentation liquid is placed in the separation chamber 101 of the separation tank 100, the weight detection component 230 is controlled to detect the weight m of the bacterial fermentation liquid in the separation tank 100 in real time, and the pressure detection component 240 is controlled to detect the pressure in the separation chamber 101 in real time and obtain the pressure value P.

[0095] The stirring component 220 is controlled to stir the bacterial fermentation liquid in the separation chamber 101, and the rotation speed of the stirring driver 224 is 200 rpm to 500 rpm. Under the continuous stirring action of the stirring component 220, the bacterial fermentation liquid evenly distributed in the separation chamber 101 forms a filter cake layer on the surface of the filter element 211 of the filter component 210, and the filtrate containing the bacteria passes through the filter cake layer and is discharged from the separation tank 100 after the primary separation of the filter component 210.

[0096] The liquid density ρ in the separation chamber 101 is calculated according to the weight m of the bacterial fermentation liquid in the separation tank body 100 detected by the weight detection component 230, and the pressure value P in the separation chamber 101 detected by the pressure detection component 240, to determine whether the primary separation is completed. The density calculation formula is: ρ = m / V, where ρ represents the liquid density in the separation chamber 101, m represents the mass (weight) of the bacterial fermentation liquid in the separation tank body 100, and V represents the volume of the bacterial fermentation liquid added to the separation chamber 101.

[0097] When it is determined that the primary separation is completed, the concentrated fermentation liquid in the separation chamber 101 is controlled to enter the rotating cylinder 320, and the rotating cylinder 320 is controlled to rotate to achieve secondary separation of the concentrated fermentation liquid to obtain solid metabolites and separation liquid containing bacterial cells. The separated solid metabolites remain in the rotating cylinder 320, and the separation liquid containing bacterial cells is discharged to the separation cylinder 310 through the separation hole of the rotating cylinder 320, and can be discharged from the external discharge outlet of the separation cylinder 310.

[0098] In some of the embodiments, when the pressure value P in the separation chamber 101 detected in real time by the pressure detection component 240 is ≥ the preset pressure value P1, it means that the filter element 211 of the filter component 210 in the separation chamber 101 is blocked, and backwash liquid is input through the backwash port 105 on the separation tank body 100 to backwash the filter element 211 of the filter component 210 until the pressure value P in the separation chamber 101 detected by the pressure detection component 240 is < the preset pressure value P2.

[0099] In some embodiments, when the pressure value P≥preset pressure value P1, it indicates that the filter element of the filter component is clogged, and the alarm mechanism is controlled to operate.

[0100] In some embodiments, the liquid return line 330 is controlled to drain the separation liquid containing the bacterial cells after the secondary separation and combine it with the filtrate containing the bacterial cells after the primary separation.

[0101] In some embodiments, the thickness of the filter cake layer is controlled to be 0.4 mm to 1.6 mm.

[0102] In some embodiments, when judging whether the primary separation is completed, the following steps are included: the liquid density ρ in the separation chamber 101 is calculated according to the weight m of the bacterial fermentation liquid in the separation tank body 100 detected by the weight detection component 230, and the relationship between the liquid density ρ and the critical density ρ0 is judged. When ρ≥ρ0 and the pressure value P<preset pressure value P0, it means that the primary separation is completed, and the concentrated fermentation liquid in the separation chamber 101 is controlled to enter the rotating cylinder 320. It should be noted that when calculating the liquid density ρ, the weight m is based on the real-time weight of the bacterial fermentation liquid in the separation tank body 100 after the primary separation at the time of judgment. Similarly, the pressure value P is based on the real-time pressure of the separation chamber 101 at the time of judgment after the primary separation.

[0103] In the bacterial fermentation broth separation and extraction method of the present invention, stirring control is added during the separation process to realize a thinner filter cake layer on the filter element 211 of the filter component 210 as a solid layer, and the thickness of the filter cake layer is controlled to be 0.4mm to 1.6mm, so that the filtration separation process is completed under a very thin filter cake layer, and most of the bacteria can be recovered in the primary separation, reducing production costs, while ensuring that the activity of the bacteria is not destroyed. Furthermore, the present application performs secondary separation on the concentrated fermentation broth after the primary separation through the secondary separation mechanism 300, directly obtaining the solid metabolites generated by the fermentation, reducing the time for the fermentation broth to be transferred between devices, and improving the separation and extraction efficiency.

[0104] Example 1

[0105] This embodiment provides a bacterial fermentation liquid separation and extraction device 10.

[0106] 1 and 2 , the bacterial fermentation liquid separation and extraction device 10 of this embodiment includes a separation tank 100 , a primary separation mechanism 200 , a secondary separation mechanism 300 , an alarm mechanism, and a control mechanism.

[0107] The bottom of the separation tank body 100 is connected with a support 107, and the separation tank body 100 is installed on a plurality of support members 107. The separation tank body 100 has a separation chamber 101. The separation tank body 100 has a liquid inlet 102, a liquid outlet 103, a pipetting port 104 and a backwash port 105 which are communicated with the separation chamber 101. The position of the liquid outlet 103 along the height direction of the separation tank body 100 is higher than the position of the liquid inlet 102. The filter component 210 is connected to the liquid outlet 103 for discharging the bacteria after the primary separation, and the separation cylinder 310 is connected to the separation chamber 101 through the pipetting port 104.

[0108] The primary separation mechanism 200 includes a filter component 210, a stirring component 220, a weight detection component 230 and a pressure detection component 240. The filter component 210 includes a filter element 211 and a liquid collecting pipe 212 arranged in the separation chamber 101. The number of filter elements 211 is multiple, and the multiple filter elements 211 are distributed at intervals. The filter element 211 has filter holes. The filter element 211 extends from the top of the separation chamber 101 to the bottom of the separation chamber 101, and the filter element 211 is spaced from the bottom of the separation chamber 101. There are several filter holes on the filter element 211, and the pore size of the filter hole is 3.5μm, which is slightly larger than the diameter of the bacteria and smaller than the diameter of the solid metabolites. The liquid collecting pipe 212 is close to the top of the separation chamber 101, and the filter element 211 is connected to the liquid collecting pipe 212 and discharges the bacteria after primary separation through the liquid collecting pipe 212. The backwash port 105 is communicated with the liquid collecting pipe 212 , and the backwash port 105 is used to backwash the clogged filter element 211 with the backwash liquid.

[0109] The stirring member 220 is connected to the separation tank body 100 for stirring the bacterial fermentation liquid in the separation chamber 101. The stirring member 220 includes a stirring shaft 221, a sealing member 222, a stirring paddle 223, a stirring driver 224, a driver support 225 and a coupling 226. A portion of the stirring shaft 221 extends into the separation chamber 101 and another portion extends to the outside of the separation tank body 100. The stirring shaft 221 is dynamically sealed with the separation tank through the sealing member 222. The stirring paddle 223 is located in the separation chamber 101 and connected to the stirring shaft 221. The stirring paddle 223 is arranged below the filter element 211 in the separation chamber 101. The driver support 225 is connected to the top of the separation tank body 100, the stirring driver 224 is installed on the driver support 225, and the stirring driver 224 is connected to the stirring shaft 221 through the coupling 226.

[0110] The weight detection component 230 is mounted on the support 107 and connected to the separation tank 100 to detect the weight m of the bacterial fermentation liquid in the separation tank 100. The pressure detection component 240 is connected to the separation tank 100 to detect the pressure in the separation chamber 101.

[0111] The secondary separation mechanism 300 includes a separation cylinder 310, a rotating cylinder 320, a liquid return pipeline 330 and a rotating drive component 340. The separation cylinder 310 is connected to the separation chamber 101, and a pneumatic valve 350 is provided on the connecting pipeline between the separation cylinder 310 and the separation tank body 100, and the connecting pipeline between the separation cylinder 310 and the separation tank body 100 is arranged in a vertical direction. The separation cylinder 310 and the rotating cylinder 320 are both arranged in an inclined manner, and the rotation axis of the rotating cylinder 320 has an angle of 45° with the vertical direction. The rotating cylinder 320 has a plurality of separation holes, and the aperture of the separation hole is larger than the diameter of the bacterial body and smaller than the diameter of the solid metabolite. The rotating cylinder 320 is rotatably arranged in the separation cylinder 310, and the rotating drive component 340 is connected to the rotating cylinder 320 to drive the rotating cylinder 320 to rotate. The liquid return pipeline 330 is connected to the separation cylinder 310 from the lowest point of the separation cylinder 310, and the liquid return pipeline 330 is used to drain the separation liquid containing the bacterial body.

[0112] The control mechanism is electrically connected to the stirring component 220 , the weight detecting component 230 , the pressure detecting component 240 , the pneumatic valve 350 , the rotation driving component 340 and the alarm mechanism.

[0113] An embodiment of the present application also provides a bacterial fermentation broth separation and extraction method for the separation and extraction of sophorolipid fermentation broth. Some parameters for separation and extraction are: the bacterial body diameter range is 0.6μm to 3.2μm, and the solid metabolite diameter range is >5μm.

[0114] A bacterial fermentation liquid separation and extraction method, using the above-mentioned bacterial fermentation liquid separation and extraction device 10, comprises the following steps:

[0115] S1. Place the bacterial fermentation liquid in the separation chamber 101 of the separation tank 100 through the liquid inlet 102 and then close the liquid inlet 102. The control mechanism controls the weight detection component 230 to detect the weight m of the bacterial fermentation liquid in the separation tank 100 in real time, and controls the pressure detection component 240 to detect the pressure in the separation chamber 101 in real time to obtain the pressure value P.

[0116] S2. The control mechanism controls the stirring component 220 to stir the bacterial fermentation liquid in the separation chamber 101. The rotation speed of the stirring driver 224 is controlled to be 300 rpm. Under the continuous stirring action of the stirring component 220, the bacterial fermentation liquid evenly distributed in the separation chamber 101 forms a filter cake layer with a thickness of 1.2 mm on the surface of the filter element 211 of the filter component 210, and the filtrate containing the bacteria passes through the filter cake layer and is discharged from the separation tank 100 after the primary separation of the filter component 210.

[0117] S3, according to the weight of the bacterial fermentation liquid in the separation tank 100 detected by the weight detection component 230, 50 kg, the initial liquid density ρ in the separation chamber 101 is calculated as 1.2 x 10 3 kg / m 3 The pressure detection component 240 detects the initial pressure value P=0.05MPa in the separation chamber 101. As the separation and extraction process proceeds, the liquid density ρ and the critical density ρ0=1.42x10 3 kg / m 3 , when ρ≥ρ0, and the pressure value P≥preset pressure value P0=0.2MPa, it indicates that the primary separation is completed, and the control mechanism turns on the pneumatic valve 350 to control the concentrated fermentation liquid in the separation chamber 101 to enter the rotating cylinder 320. When the pressure value P in the separation chamber 101 detected by the pressure detection component 240≥preset pressure value P1=0.4MPA, it indicates that the filter element 211 of the filter component 210 in the separation chamber 101 is blocked. At this time, the control mechanism controls the stirring component 220 to stop stirring, and inputs backwashing liquid through the backwashing port 105 on the separation tank body 100 to backwash the filter element 211 of the filter component 210, until the pressure value P in the separation chamber 101 detected by the pressure detection component 240 in real time is less than the preset pressure value P2=0.02MPa.

[0118] S4. When ρ≥ρ0 and the pressure value P<preset pressure value P0, it is determined that the primary separation is completed. At this time, the control mechanism opens the pneumatic valve 350 to allow the concentrated fermentation liquid in the separation chamber 101 to enter the rotating cylinder 320.

[0119] S5. The control mechanism controls the rotary drive component 340 to drive the rotating drum 320 to rotate at a speed of 1500 rpm to separate and concentrate the separated liquid containing bacteria in the fermentation liquid. The return liquid pipeline 330 is controlled to open and drain the separated liquid containing bacteria after the secondary separation. The separated liquid containing bacteria drained by the return liquid pipeline 330 is combined with the filtrate of bacteria after the primary separation. The separation chamber 101 is opened to obtain solid metabolites.

[0120] S6. Perform activity detection on the separated bacteria. The separated bacteria are added to the newly cultured sophorolipid fermentation broth, and sophorolipids can be produced normally. The content of sophorolipids in the fermentation broth was measured after the same fermentation time of 150h. The specific comparison data was: the sophorolipid content in the initial fermentation broth was about 408g / L, and the sophorolipid content in the fermentation broth of the secondary fermentation of the separated bacteria was about 376g / L. It can be proved that the separated bacteria have strong activity, meet the needs of actual production, and can achieve the reuse of bacteria.

[0121] In summary, the bacterial fermentation liquid separation and extraction device 10 of the present invention can realize the efficient extraction and recovery of bacterial cells and the rapid separation of solid metabolites, and the recovered bacterial cells are reused to reduce production costs. The bacterial fermentation liquid separation and extraction method of the present invention can efficiently complete the recovery of bacterial cells and the separation of solid metabolites when processing fermentation liquid with a small density difference between bacterial cells and solid metabolites. During the separation process, most of the bacterial cells can be recovered through the primary recovery of the separation chamber 101 under the premise of well protecting the bacterial cells, and the solid metabolites can be directly obtained through the secondary separation, which greatly improves the efficiency of separating solid metabolites.

[0122] In the above embodiments, the description of each embodiment has its own emphasis. For parts that are not described in detail in a certain embodiment, reference can be made to the relevant descriptions of other embodiments.

[0123] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0124] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A bacterial fermentation liquid separation and extraction device, characterized in that: It comprises a separation tank body, a primary separation mechanism and a secondary separation mechanism; the separation tank body has a separation chamber that can be sealed; The primary separation mechanism includes a filtering component, a stirring component, a weight detection component and a pressure detection component. The filtering component is arranged in the separation chamber to perform primary separation on the bacterial fermentation liquid to obtain bacterial bodies and concentrated fermentation liquid. The stirring component is connected to the separation tank body to stir the bacterial fermentation liquid in the separation chamber. The weight detection component is used to detect the weight of the bacterial fermentation liquid in the separation tank body. The pressure detection component is used to detect the pressure in the separation chamber. The secondary separation mechanism includes a separation cylinder and a rotating cylinder. The rotating cylinder is connected to the separation chamber to collect the concentrated fermentation liquid after the primary separation. The rotating cylinder has a plurality of separation holes. The aperture of the separation holes is larger than the diameter of the bacteria and smaller than the diameter of the solid metabolites. The rotating cylinder can be rotatably arranged in the separation cylinder. The rotating cylinder can be rotated to realize the secondary separation of the concentrated fermentation liquid.

2. The bacterial fermentation liquid separation and extraction device according to claim 1, characterized in that: The separation tank body also has a liquid inlet, a liquid outlet and a pipetting port connected to the separation chamber. The position of the liquid outlet along the height direction of the separation tank body is higher than the position of the liquid inlet. The filter component is connected to the liquid outlet, and the separation cylinder is connected to the separation chamber through the pipetting port.

3. The bacterial fermentation liquid separation and extraction device according to claim 1, characterized in that: The filter component includes a filter element and a liquid collection pipe arranged in the separation chamber, the filter element has a plurality of filter holes, the pore size of the filter holes is larger than the diameter of the bacteria and smaller than the diameter of the solid metabolic products, and the filter element is connected to the liquid collection pipe and can pass through the liquid collection pipe.

4. The bacterial fermentation liquid separation and extraction device according to claim 3, characterized in that: The bacterial fermentation liquid separation and extraction device also meets at least one of the following conditions: (1) There are multiple filter elements, and the multiple filter elements are distributed at intervals in the separation chamber; (2) The filter element extends from the top of the separation tank body to the bottom of the separation tank body, and there is a gap between the filter element and the bottom of the separation tank body; (3) The liquid collecting pipe is close to the top of the separation tank; (4) The separation tank body also has a backwash port, which is communicated with the liquid collecting pipe and is used to connect an external backwash liquid to backwash the clogged filter element.

5. The bacterial fermentation liquid separation and extraction device according to any one of claims 1 to 4, characterized in that: The stirring component includes a stirring shaft, a sealing member, a stirring paddle and a stirring driver. A portion of the stirring shaft extends into the separation chamber and another portion extends to the outside of the separation tank body. The stirring shaft is dynamically sealed with the separation tank through the sealing member. The stirring paddle is located in the separation chamber and connected to the stirring shaft. The stirring driver is installed on the separation tank body and connected to the stirring shaft.

6. The bacterial fermentation liquid separation and extraction device according to claim 5, characterized in that: The bacterial fermentation liquid separation and extraction device also meets at least one of the following conditions: (1) The stirring paddle is arranged below the filter component and close to the bottom of the separation chamber; (2) The stirring component further includes a driver support, the driver support is connected to the top of the separation tank, and the stirring driver is installed on the driver support; (3) The stirring component further includes a coupling, and the stirring drive is connected to the stirring shaft via the coupling.

7. The bacterial fermentation liquid separation and extraction device according to any one of claims 1 to 4 and 6, characterized in that: The bacterial fermentation liquid separation and extraction device also meets at least one of the following conditions: (1) A support is connected to the bottom of the separation tank body, the separation tank body is mounted on a plurality of the support members, and the weight detection component is mounted on the support member and connected to the separation tank body; (2) A pneumatic valve is provided on the connecting pipeline between the separation cylinder and the separation tank body; (3) The connecting pipe between the separation cylinder and the separation tank body is arranged in a vertical direction, the separation cylinder and the rotating cylinder are arranged in an inclined manner, and the rotating axis of the rotating cylinder has an angle with the vertical direction; (4) The secondary separation mechanism further includes a liquid return pipeline, which is connected to the separation cylinder and is used to drain the separation liquid containing bacterial cells after the secondary separation; (5) The secondary separation mechanism further includes a rotation driving component, which is connected to the rotating drum to drive the rotating drum to rotate; (6) The bacterial fermentation liquid separation and extraction device further comprises an alarm mechanism, which is connected to the pressure detection component by signal. When the pressure value in the separation chamber detected by the pressure detection component is greater than a preset pressure value, the alarm mechanism operates.

8. A method for separating and extracting bacterial fermentation broth, characterized in that: The bacterial fermentation liquid separation and extraction device according to any one of claims 1 to 7 comprises the following steps: Placing the bacterial fermentation liquid in the separation chamber of the separation tank, controlling the weight detection component to detect the weight m of the bacterial fermentation liquid in the separation tank in real time, and controlling the pressure detection component to detect the pressure value P in the separation chamber in real time; Controlling the stirring component to stir the bacterial fermentation liquid in the separation chamber, so that the bacterial fermentation liquid forms a filter cake layer on the surface of the filter element of the filter component, and the filtrate containing the bacteria passes through the filter cake layer and is discharged from the separation tank after the primary separation of the filter component; Determine whether the primary separation is completed according to the pressure value P and the liquid density ρ in the separation chamber calculated from the weight m of the bacterial fermentation liquid in the separation tank body; After the primary separation is completed, the concentrated fermentation liquid in the separation chamber is controlled to enter the rotating drum, and the rotating drum is controlled to rotate to achieve secondary separation of the concentrated fermentation liquid to obtain solid metabolites and separation liquid containing bacteria.

9. The method for separating and extracting bacterial fermentation broth according to claim 8, characterized in that: The bacterial fermentation broth separation and extraction method further comprises at least one of the following steps: (1) When the pressure value P ≥ the preset pressure value P1, it indicates that the filter element of the filter component is clogged, and a backwashing liquid is input through the backwashing port on the separation tank body to backwash the filter element of the filter component until the pressure value P < the preset pressure value P2; (2) When the pressure value P ≥ the preset pressure value P1, it indicates that the filter element of the filter component is clogged, and the control alarm mechanism works; (3) Control the return liquid pipeline to drain the separated liquid containing bacteria after the secondary separation and merge it with the filtrate containing bacteria after the primary separation; (4) The thickness of the filter cake layer is controlled to be 0.4 mm to 1.6 mm.

10. The method for separating and extracting bacterial fermentation broth according to claim 8 or 9, characterized in that: When judging whether the primary separation is completed, the following steps are included: calculating the liquid density ρ in the separation chamber according to the weight m of the bacterial fermentation liquid in the separation tank body, judging the relationship between the liquid density ρ and the critical density ρ0, when ρ≥ρ0 and the pressure value P<preset pressure value P0, it indicates that the primary separation is completed.

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