Bacterial fermentation broth separation and extraction device and separation and extraction method thereof

Abstract

The application discloses a bacterial fermentation liquid separation and extraction device and a separation and extraction method. The bacterial fermentation liquid separation and extraction device comprises a separation tank body, a first separation mechanism and a second separation mechanism, and the separation tank body has a separation cavity; the first separation mechanism comprises a filtering part, a stirring part, a weight detection part and a pressure detection part, the filtering part is provided with filtering holes, the filtering part is arranged in the separation cavity, the stirring part is connected to the separation tank body, the weight detection part is connected to the separation tank body, and the pressure detection part is connected to the separation tank body; the second separation mechanism comprises a separation cylinder and a rotating cylinder, the separation cylinder is communicated with the separation cavity, the rotating cylinder is provided with a plurality of separation holes, and the rotating cylinder is rotatably arranged in the separation cylinder. The bacterial fermentation liquid separation and extraction device can realize efficient extraction and recovery of bacterial bodies and rapid separation of solid metabolites, the recovered bacterial bodies can be reused, and the production cost is low.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of bioengineering and fermentation liquid separation, in particular to a bacterial fermentation liquid separation and extraction device and a separation and extraction method thereof. BACKGROUND

[0002] Fermentation is a process of preparing microbial cells, direct metabolites or secondary metabolites by means of microbial life activities under aerobic or anaerobic conditions. Fermentation is widely used in food industry, biological and chemical industry. At present, fermentation is carried out by large-scale growth culture of microorganisms or animal cells, plant cells, so as to produce and accumulate a large amount of metabolites required for fermentation.

[0003] In the process of bacterial fermentation liquid treatment, for high-value bacteria, the high-value bacteria mixed in the fermentation product need to be recovered and separated from the solid components in the fermentation liquid. The bacterial separation method in the traditional technology generally adopts the methods of filtration, centrifugal separation or precipitation. Among them, the separation methods of filtration and precipitation are prone to block the filter screen or filter membrane due to the small diameter of bacteria in the separation process, resulting in long separation time and unsatisfactory separation effect; the separation effect is poor when the density of the bacterial body is close to the density of the solid in the fermentation liquid in the centrifugal separation method. SUMMARY

[0004] Therefore, it is necessary to provide a bacterial fermentation liquid separation and extraction device.

[0005] An embodiment of the present application provides a bacterial fermentation liquid separation and extraction device.

[0006] A bacterial fermentation liquid separation and extraction device, comprising a separation tank body, a first-stage separation mechanism and a second-stage separation mechanism, the separation tank body has a separable separation cavity;

[0007] The first-stage separation mechanism comprises a filter component, a stirring component, a weight detection component and a pressure detection component, the filter component is arranged in the separation cavity for first-stage separation of the bacterial fermentation liquid to obtain the bacterial body and the concentrated fermentation liquid, the stirring component is connected to the separation tank body for stirring the bacterial fermentation liquid in the separation cavity, the weight detection component is used for detecting the weight m of the bacterial fermentation liquid in the separation tank body, and the pressure detection component is used for detecting the pressure in the separation cavity;

[0008] The secondary separation mechanism comprises a separation cylinder and a rotating cylinder, the rotating cylinder is communicated with the separation cavity for collecting the concentrated fermentation liquid after the primary separation, the rotating cylinder has a plurality of separation holes, the diameter of the separation holes is greater than the diameter of the bacteria and less than the diameter of the solid metabolites, the rotating cylinder is rotatably arranged in the separation cylinder, and the rotating cylinder can rotate to realize the secondary separation of the concentrated fermentation liquid to obtain the solid metabolites and the separation liquid containing the bacteria.

[0009] The bacterial fermentation liquid separation and extraction device can realize efficient extraction and recovery of the bacteria and rapid separation of the solid metabolites, the recovered bacteria can be reused, and the production cost is reduced.

[0010] In some embodiments, the separation tank body further has a liquid inlet, a liquid outlet and a liquid transfer port communicated with the separation cavity, the position of the liquid outlet is higher than that of the liquid inlet along the height direction of the separation tank body, the filter component is communicated with the liquid outlet, and the separation cylinder is communicated with the separation cavity through the liquid transfer port.

[0011] In some embodiments, the filter component comprises a filter core arranged in the separation cavity and a liquid collecting pipeline, the filter core has a plurality of filter holes, the diameter of the filter holes is greater than the diameter of the bacteria and less than the diameter of the solid metabolites, and the filter core is connected to and can pass through the liquid collecting pipeline.

[0012] In some embodiments, the number of the filter cores is a plurality, and the plurality of filter cores are distributed at intervals in the separation cavity.

[0013] In some embodiments, the filter core extends from the top of the separation tank body to the bottom of the separation tank body, and the filter core has an interval from the bottom of the separation tank body.

[0014] In some embodiments, the liquid collecting pipeline is close to the top of the separation tank body.

[0015] In some embodiments, the separation tank body further has a backwashing port communicated with the liquid collecting pipeline, and the backwashing port is used for connecting a backwashing liquid to backflush the blocked filter core.

[0016] In some embodiments, the stirring component comprises a stirring shaft, a sealing member, a stirring paddle and a stirring driver, one part of the stirring shaft extends into the separation cavity and the other part extends to the outside of the separation tank body, the stirring shaft is sealed with the separation tank body through the sealing member, the stirring paddle is located in the separation cavity and connected to the stirring shaft, and the stirring driver is installed on the separation tank body and connected to the stirring shaft.

[0017] In some embodiments, the stirring paddle is arranged below the filter component and close to the bottom of the separation cavity.

[0018] In some embodiments, the stirring component further comprises a driver support connected to the top of the separation tank body, and the stirring driver is mounted on the driver support.

[0019] In some embodiments, the stirring component further comprises a coupling, and the stirring driver is connected with the stirring shaft through the coupling.

[0020] In some embodiments, the bottom of the separation tank body is connected with supports, the separation tank body is mounted on the supports, and the weight detection component is mounted on the supports and connected with the separation tank body.

[0021] In some embodiments, a pneumatic valve is arranged on the communication pipeline between the separation cylinder and the separation tank body.

[0022] In some embodiments, the communication pipeline between the separation cylinder and the separation tank body is arranged in a vertical direction, the separation cylinder and the rotating cylinder are both arranged in an inclined manner, and the rotating shaft of the rotating cylinder has an angle with the vertical direction.

[0023] In some embodiments, the secondary separation mechanism further comprises a liquid return pipeline connected with the separation cylinder for draining the separation liquid containing the bacterial bodies after the secondary separation.

[0024] In some embodiments, the secondary separation mechanism further comprises a rotating driving component connected with the rotating cylinder for driving the rotating cylinder to rotate.

[0025] In some embodiments, the bacterial fermentation liquid separation and extraction device further comprises a control mechanism electrically connected with the stirring component, the weight detection component, and the pressure detection component.

[0026] In some embodiments, the bacterial fermentation liquid separation and extraction device further comprises an alarm mechanism signal connected with the pressure detection component, and the alarm mechanism works when the pressure value in the separation cavity detected by the pressure detection component is greater than a preset pressure value.

[0027] An embodiment of the present application further provides a bacterial fermentation liquid separation and extraction method.

[0028] A bacterial fermentation liquid separation and extraction method using the bacterial fermentation liquid separation and extraction device described above, comprising the following steps:

[0029] The bacterial fermentation liquid is placed in a separation cavity of a separation tank, a weight detection component detects the weight m of the bacterial fermentation liquid in the separation tank in real time, and a pressure detection component detects the pressure value P in the separation cavity in real time;

[0030] A stirring component is controlled to stir the bacterial fermentation liquid in the separation cavity, the bacterial fermentation liquid forms a filter cake layer on the surface of a filter element of a filter component, and the filtrate containing bacterial bodies passes through the filter cake layer and is separated by the filter component to be discharged out of the separation tank;

[0031] According to the pressure value P and the liquid density ρ in the separation cavity calculated from the weight m of the bacterial fermentation liquid in the separation tank, it is determined whether the primary separation is completed.

[0032] When it is determined that the primary separation is completed, the concentrated fermentation liquid in the separation cavity is controlled to enter a rotating drum, and the rotating drum is controlled to rotate to realize secondary separation of the concentrated fermentation liquid to obtain solid metabolites and separation liquid containing bacterial bodies.

[0033] In some embodiments, when the pressure value P is greater than or equal to the preset pressure value P1, it indicates that the filter element of the filter component is blocked, backwashing liquid is input through a backwashing port on the separation tank to backwash the filter element of the filter component, and the process is continued until the pressure value P is less than the preset pressure value P2.

[0034] In some embodiments, when the pressure value P is greater than or equal to the preset pressure value P1, it indicates that the filter element of the filter component is blocked, and an alarm mechanism is controlled to work.

[0035] In some embodiments, a liquid return pipeline is controlled to guide the separation liquid containing bacterial bodies after the secondary separation to be combined with the filtrate containing bacterial bodies after the primary separation.

[0036] In some embodiments, the thickness of the filter cake layer is controlled to be 0.4 mm to 1.6 mm.

[0037] In some embodiments, when it is determined whether the primary separation is completed, the following steps are included: the liquid density ρ in the separation cavity is calculated from the weight m of the bacterial fermentation liquid in the separation tank, the relationship between the liquid density ρ and the critical density ρ0 is determined, when ρ is greater than or equal to ρ0 and the pressure value P is less than the preset pressure value P0, it indicates that the primary separation is completed.

[0038] The bacterial fermentation liquid separation and extraction device can solve the problems that the centrifugal separation method is difficult to separate when the density difference between the bacteria and the solid metabolites in the fermentation liquid is very small, and the filter part filter screen or filter membrane is easily blocked when the conventional filtering or sedimentation method is used. The application strictly controls the thickness of the solid layer such as filter cake layer in the separation process by setting a closed separation environment, ensures that the activity of most of the bacteria recovered by primary separation is not affected, can meet the strict requirements of the bacteria on the living environment, and realizes efficient extraction and recovery of the bacteria and rapid separation of the solid metabolites under the condition that the bacteria are difficult to survive in the unsterilized air environment and the solid sediment, the recovered bacteria can be reused, and the production cost can be greatly reduced.

[0039] The bacterial fermentation liquid separation and extraction device of the application increases stirring control in the separation process, realizes the filter cake layer with a thin core layer on the filter core of the filter part, controls the thickness of the filter cake layer to be 0.4mm-1.6mm, and makes the filtering and separation process be completed under the very thin filter cake layer, so that most of the bacteria can be recovered in the primary separation, the production cost is reduced, and the activity of the bacteria is ensured not to be destroyed. Further, the application realizes secondary separation of the solid metabolites contained in the concentrated fermentation liquid after the primary separation by the secondary separation mechanism, directly obtains the solid metabolites generated by fermentation, reduces the time of transferring the fermentation liquid between devices, and improves the separation and extraction efficiency. BRIEF DESCRIPTION OF DRAWINGS

[0040] In order to more clearly illustrate the technical solutions in the embodiments of the application, the following will briefly introduce the drawings needed to be used in the embodiment description. Obviously, the drawings in the following description are only some embodiments of the application, and other drawings can be obtained by those skilled in the art without creative labor.

[0041] In order to more completely understand the application and its beneficial effects, the following will be described with reference to the drawings. In the following description, the same reference numerals represent the same parts.

[0042] Figure 1 The bacterial fermentation liquid separation and extraction device described in an embodiment of the application is shown in the schematic diagram.

[0043] Figure 2 The bacterial fermentation liquid separation and extraction device described in an embodiment of the application is shown in the schematic diagram.

[0044] Explanation of reference numerals

[0045] 10, bacterial fermentation liquid separation and extraction device; 100, separation tank body; 101, separation cavity; 102, liquid inlet; 103, liquid outlet; 104, pipette port; 105, backwash port; 106, baffle; 107, support; 108, connecting cylinder; 109, cover plate; 200, primary separation mechanism; 210, filter component; 211, filter core; 212, liquid collection pipeline; 220, stirring component; 221, stirring shaft; 222, sealing member; 223, stirring paddle; 224, stirring driver; 225, driver support; 226, coupling; 230, weight detection component; 240, pressure detection component; 300, secondary separation mechanism; 310, separation cylinder; 320, rotating cylinder; 330, liquid return pipeline; 340, rotating drive component; 350, pneumatic valve. DETAILED DESCRIPTION

[0046] In order to make the above objectives, characteristics and advantages of the present application more obvious and easy to understand, the specific embodiments of the present application are described in detail below with reference to the drawings. In the following description, many specific details are set forth in order to provide a thorough understanding of the present application. However, the present application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without departing from the concept of the present application, so the present application is not limited to the specific embodiments disclosed below.

[0047] In the description of the present application, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the purpose of facilitating the description of the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application.

[0048] In the present application, unless otherwise explicitly specified and limited, the terms "mounting", "connection", "connection", "fixing" and the like should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or it can be integrated; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the internal communication of two elements or the interaction relationship between two elements, unless otherwise explicitly limited. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0049] In the present application, unless otherwise explicitly specified and limited, a first feature is "on" or "under" a second feature can mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature "over", "above" and "on top of" the second feature can mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is horizontally higher than the second feature. The first feature "under", "below" and "underneath" the second feature can mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is horizontally lower than the second feature.

[0050] In the description of the present application, if the meaning of several is more than one, the meaning of multiple is more than two, greater than, less than, more than, etc. are understood as not including the number, above, below, within, etc. are understood as including the number. If it is described that the first, second is only used for the purpose of distinguishing technical features, it cannot be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features or the sequence of indicated technical features.

[0051] In this paper, "optionally", "optional", "optional" means optional, that is, optional from "have" or "no" two parallel schemes. If there are multiple "optional" in a technical solution, unless otherwise specified, and there is no contradiction or mutual restriction, each "optional" is independent of each other. In this application, "optionally contains", "optionally contains" and the like are described as "contains or does not contain".

[0052] In this application, if a numerical interval (i.e. a numerical range) is involved, unless otherwise specified, the distribution of optional values in the numerical interval is considered to be continuous and includes both numerical endpoints (i.e. the minimum value and the maximum value) of the numerical interval and every value between the two numerical endpoints. If no special instructions are given, when the numerical interval only points to the integers in the numerical interval, including the two endpoint integers of the numerical range and every integer between the two endpoints, it is equivalent to directly listing each integer. When multiple numerical ranges are provided to describe a feature or characteristic, these numerical ranges can be combined. In other words, unless otherwise indicated, the numerical range disclosed in this application should be understood to include any and all sub-ranges included therein. The "numerical value" in the numerical interval can be any quantitative value, such as a number, a percentage, a ratio, etc. The "numerical interval" allows a broad definition of the quantitative interval, such as the percentage interval, the ratio interval, the ratio interval, etc.

[0053] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein in the specification of this invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0054] This application provides a bacterial fermentation broth separation and extraction device 10 to solve at least one of the following problems in the existing bacterial fermentation broth separation methods: (1) When using filtration or sedimentation methods, due to the small diameter of the bacteria, the solid metabolites in the fermentation broth easily clog the filter screen or filter membrane during the separation process, resulting in excessively long separation time and unsatisfactory separation effect; (2) When using centrifugation separation methods, the separation effect is poor when the density of the bacteria and the density of the solids in the fermentation broth are not significantly different. The bacterial fermentation broth separation and extraction device 10 will be described below with reference to the accompanying drawings.

[0055] The bacterial fermentation broth separation and extraction device 10 of this application can be used for the separation of bacterial cells from bacterial fermentation broth. The separation and extraction method of this invention reduces production costs by reusing the recovered bacterial cells, and improves separation efficiency by completing two-stage separation in one device to directly obtain solid metabolites. The bacterial fermentation broth separation and extraction device 10 and the separation and extraction method provided in this application are suitable for processing bacterial fermentation broths with a small density difference between bacterial cells and solid metabolites, and can efficiently complete the recovery of bacterial cells and the separation of solid metabolites. It should be noted that the diameter of the bacterial cells to be separated in this application is smaller than the diameter of the solid metabolites.

[0056] To more clearly illustrate the structure of the bacterial fermentation broth separation and extraction device 10, the following description, in conjunction with the accompanying drawings, will explain the device. For example, please refer to... Figure 1 As shown, Figure 1 This is a schematic diagram of the structure of the bacterial fermentation broth separation and extraction device 10 provided in the embodiments of this application.

[0057] For example, please refer to Figure 1 As shown, a bacterial fermentation broth separation and extraction device 10 includes a separation tank 100, a primary separation mechanism 200, and a secondary separation mechanism 300. The separation tank 100 has a sealable separation chamber 101.

[0058] Please see Figure 1 and Figure 2 As shown, Figure 2This is a cross-sectional structural diagram of a bacterial fermentation broth separation and extraction device 10 according to an embodiment of the present invention. The primary separation mechanism 200 includes a filter element 210, a stirring element 220, a weight detection element 230, and a pressure detection element 240. The filter element 210 is disposed in the separation chamber 101 for primary separation of the bacterial fermentation broth to obtain bacterial cells and concentrated fermentation broth. The stirring element 220 is connected to the separation tank 100 for stirring the bacterial fermentation broth in the separation chamber 101. The weight detection element 230 is used to detect the weight m of the bacterial fermentation broth in the separation tank 100, and the weight detection element 230 can be connected to the separation tank 100. The pressure detection element 240 is used to detect the pressure in the separation chamber 101 and obtain a real-time pressure value P. The pressure value P is used to determine whether the filter element in the separation chamber is clogged and whether the primary separation is completed. The pressure detection element 240 can be connected to the separation tank 100.

[0059] Please see Figure 1 and Figure 2 As shown, the secondary separation mechanism 300 includes a separation cylinder 310 and a rotating cylinder 320. The rotating cylinder 320 is connected to the separation chamber 101. The rotating cylinder 320 has several separation holes, the diameter of which is larger than the diameter of the bacterial cells and smaller than the diameter of the solid metabolites. The rotating cylinder 320 is rotatably disposed within the separation cylinder 310. The rotating cylinder 320 can rotate to achieve secondary separation of the concentrated fermentation broth to obtain solid metabolites and a separation liquid containing bacterial cells. Through the rotation of the rotating cylinder 320, the concentrated fermentation broth within the rotating cylinder 320 can be centrifuged to obtain solid metabolites and a separation liquid containing bacterial cells. The separation liquid containing bacterial cells can be refluxed back into the separation chamber for primary separation, or directly combined with the filtrate containing bacterial cells after primary separation, and the recovered bacterial cells can be reused.

[0060] The aforementioned bacterial fermentation broth separation and extraction device 10 solves the problems of difficulty in achieving separation by centrifugation when the density difference between bacteria and solid metabolites in the fermentation broth is small, and the easy clogging of the filter screen or membrane of the filter element 210 by conventional filtration or sedimentation methods. This application, by setting up a sealed separation environment and strictly controlling the thickness of the solid layer, such as the filter cake layer, during the separation process, ensures that the activity of most of the recovered bacteria remains unaffected. It meets the stringent requirements of bacteria for their living environment and enables efficient extraction and recovery of bacteria and rapid separation of solid metabolites under conditions where bacteria struggle to survive in unsterilized air and solid sediment. The recovered bacteria can be reused, significantly reducing production costs.

[0061] In some of these embodiments, please refer to Figure 2As shown, the separation tank 100 also has a liquid inlet 102, a liquid outlet 103 and a liquid transfer port 104 which are in communication with the separation chamber 101. The liquid outlet 103 is located higher than the liquid inlet 102 along the height direction of the separation tank 100. The filter component 210 is connected to the liquid outlet 103 for primary separation, and the separation cylinder 310 is connected to the separation chamber 101 through the liquid transfer port 104.

[0062] In some embodiments, referring to Figure 2 As shown, the separation tank 100 also has a baffle 106. The baffle 106 is located near the lower part of the separation chamber 101. The baffle 106 is used to provide a certain shear dispersion effect for the fermentation liquid during stirring. The size of the baffle 106 should not be too large, so as to be able to improve the shear dispersion effect. Preferably, the baffle 106 can be multiple, and the multiple baffles 106 are arranged at intervals. The shape and size of the baffle 106 are not limited, as long as the baffle 106 can achieve flow disturbance and improve the shear dispersion effect.

[0063] In some embodiments, referring to Figure 1 and Figure 2 As shown, the top of the separation tank 100 is connected with a connecting cylinder 108 and a cover plate 109. The cover plate 109 can be detachably connected with the connecting cylinder 108. The opening of the cover plate 109 can open the separation tank 100, so as to realize the detection, maintenance, replacement, cleaning and other processes of the various mechanisms in the separation chamber 101.

[0064] In some embodiments, referring to Figure 2 As shown, the filter component 210 has filter holes. The pore size of the filter holes is greater than the diameter of the bacteria and smaller than the diameter of the solid metabolites. In a specific example, the filter component 210 includes a filter core 211 arranged in the separation chamber 101 and a liquid collecting pipeline 212. The filter core 211 has a plurality of filter holes, and the filter core 211 is connected to and can pass through the liquid collecting pipeline 212. It should be noted that the diameter of the bacteria generally refers to the maximum diameter of the bacteria, and the diameter of the solid metabolites generally refers to the minimum diameter of the solid metabolites.

[0065] In this application, the pore size of the filter holes is slightly larger than the diameter of the bacteria and smaller than the diameter of the solid metabolites, so as to improve the filtering precision of the filter core 211. The size of the bacteria is about 0.6 μm to 3.2 μm, and the diameter of the solid metabolites is generally more than 5 μm. Therefore, the pore size of the filter core 211 is slightly larger than 3 μm, for example, the pore size of the filter core 211 is 3.1 μm to 3.5 μm, so as to ensure efficient filtration of the bacteria. The pore size of the separation hole can be 3.1 μm to 3.5 μm.

[0066] In some embodiments, the number of filter cores 211 is multiple. The multiple filter cores 211 are distributed at intervals in the separation chamber 101. Referring to the drawings,Figure 2 As shown, the filter cartridges 211 are evenly distributed in the separation chamber 101. Figure 2 As shown, the filter cartridges 211 are evenly distributed in the separation chamber 101. Figure 2 The example shown is only one of the examples, but cannot be used to limit the actual number of filter cartridges 211.

[0067] In some embodiments, the filter cartridges 211 are evenly spaced in the separation chamber 101. The evenly distributed filter cartridges 211 can maximize the separation of the bacteria in the separation chamber 101, improving the separation efficiency and effect.

[0068] In some embodiments, the filter cartridges 211 extend from the top of the separation tank body 100 to the bottom of the separation tank body 100, and the filter cartridges 211 have a spacing from the bottom of the separation tank body 100. Preferably, the length of the filter cartridges 211 occupies 50%-90% of the height of the separation tank body 100. Further preferably, the length of the filter cartridges 211 occupies 70%-90% of the height of the separation tank body 100.

[0069] In some embodiments, the liquid collecting pipe 212 is close to the top of the separation tank body 100. Preferably, please refer to Figure 2 As shown, the liquid collecting pipe 212 is arranged in a horizontal state in the separation chamber 101.

[0070] In some embodiments, please refer to Figure 2 As shown, the separation tank body 100 also has a backwashing port 105, which is communicated with the liquid collecting pipe 212, and the backwashing port 105 is used to connect a backwashing liquid to backflush the clogged filter cartridges 211. The backwashing port 105 can be connected to the backwashing liquid, and by setting a certain pressure of the backwashing liquid, the liquid collecting pipe 212 and the filter cartridges 211 can be backflushed, avoiding or reducing the clogging of the filter cartridges 211, and improving the service life of the filter cartridges 211. The backwashing liquid can be a specific cleaning liquid or deionized water, etc., and the backwashing liquid will not adversely affect the bacteria and solid metabolites in the separation chamber 101.

[0071] In some embodiments, please refer to Figure 2 As shown, the stirring component 220 includes a stirring shaft 221, a seal 222, a stirring paddle 223, and a stirring driver 224. The stirring shaft 221 extends into the separation chamber 101 and extends to the outside of the separation tank body 100. The stirring shaft 221 is sealed with the separation tank by the seal 222. The stirring paddle 223 is located in the separation chamber 101 and is connected to the stirring shaft 221. The stirring driver 224 is installed on the separation tank body 100 and is connected to the stirring shaft 221.

[0072] In some embodiments, the stirring driver 224 can be a stirring motor.

[0073] In some embodiments, the stirring driver 224 rotates at a speed of 200 rpm to 1000 rpm. The speed of the stirring driver 224 can be set according to actual needs. The structure of the stirring paddle 223 should avoid strong shear force on the fermentation broth, and the speed of the stirring driver 224 should not be too high to avoid loss of bacterial bodies due to excessive shear force. Preferably, the stirring driver 224 rotates at a speed of 400 rpm to 800 rpm.

[0074] In some embodiments, the stirring paddle 223 is arranged below the filter member 210 and close to the bottom of the separation tank body 100 in the separation cavity 101. The stirring paddle 223 is as close as possible to the bottom surface of the separation tank body 100, which can improve the liquid flow efficiency and effect of the fermentation broth in the separation cavity.

[0075] In some embodiments, as shown in Figure 1 and Figure 2 , the stirring member 220 further comprises a driver support 225. The driver support 225 is connected to the top of the separation tank body 100. The stirring driver 224 is installed on the driver support 225.

[0076] In some embodiments, as shown in Figure 2 , the stirring member 220 further comprises a coupling 226. The stirring driver 224 and the stirring shaft 221 are connected through the coupling 226. The coupling 226 is provided to facilitate the disassembly, maintenance, cleaning and other processes between the stirring driver 224 and the stirring shaft 221.

[0077] In some embodiments, as shown in Figure 1 and Figure 2 , the bottom of the separation tank body 100 is connected with a support 107. The separation tank body 100 is installed on a plurality of supports 107, and the weight detection member 230 is installed on the support 107 and connected with the separation tank body 100. Preferably, the plurality of supports 107 are detachably connected with the separation tank body 100.

[0078] In some embodiments, the support 107 can be a support leg. Preferably, as shown in Figure 1 and Figure 2 , the support 107 has a long strip structure.

[0079] In some embodiments, a pneumatic valve 350 is arranged on the communication pipeline between the separation cylinder 310 and the separation tank body 100. The pneumatic valve 350 is a device driven by compressed air to open or close the valve. For example, the main components of the pneumatic valve 350 include: valve body: the main structure of the valve, usually made of metal or other corrosion-resistant materials, with fluid passages inside. Actuator: the part that converts the pressure of compressed air into mechanical motion, which can open or close the valve through linear motion or rotary motion. Positioner: used to adjust the opening position of the valve to ensure that the valve can be accurately opened to the specified angle as needed. Air supply processing unit: provides clean and dry compressed air and can adjust the pressure. Accessories: such as limit switches, solenoid valves, etc., used to achieve more complex functions and controls.

[0080] In some embodiments, the pneumatic valve 350 can be selected from (1) a pneumatic ball valve: controlling the flow of fluid by rotating a ball with a hole. (2) Pneumatic butterfly valve: using a disc-shaped flapper to control the flow of fluid. (3) Pneumatic stop valve: using the up and down movement of the valve stem to drive the valve to control the on-off of the fluid. (4) Pneumatic diaphragm valve: controlling the flow of fluid by deforming the diaphragm.

[0081] In some embodiments, the secondary separation mechanism 300 includes a liquid return pipeline 330. The liquid return pipeline 330 is connected to the separation cylinder 310 for guiding the separation liquid containing the bacterial bodies after the secondary separation.

[0082] In some embodiments, the liquid return pipeline 330 is a liquid return manifold.

[0083] In some embodiments, the secondary separation mechanism 300 includes a rotary drive component 340. The separation cylinder 310 is connected to the rotary cylinder 320 for driving the rotary cylinder 320 to rotate. Optionally, the rotation speed of the rotary cylinder 320 can be 1000 rpm to 3000 rpm. Preferably, the rotation speed of the rotary cylinder 320 can be 1000 rpm to 1500 rpm.

[0084] In some embodiments, the communication pipeline between the separation cylinder 310 and the separation tank body 100 is arranged in a vertical direction. The separation cylinder 310 and the rotary cylinder 320 are both arranged obliquely, the rotary axis of the rotary cylinder 320 has an angle with the vertical direction, and the liquid return pipeline 330 is connected to the separation cylinder 310 from the bottom of the separation cylinder 310. Generally, after the separation tank body 100 is installed, the separation tank body 100 is arranged in a vertical direction as a whole, and in the height direction, the primary separation mechanism 200 is located above and the secondary separation mechanism 300 is located below, so that the concentrated fermentation liquid after the primary separation of the primary separation mechanism 200 enters the secondary separation mechanism 300 under the action of gravity.

[0085] In some embodiments, please refer to Figure 2 As shown, the angle between the rotation axis of the rotating cylinder 320 and the vertical direction is 30°-60°, so that the rotating cylinder 320 is in an inclined state, ensuring that the separation liquid containing bacteria in the rotating cylinder 320 can be discharged under the action of gravity. For example, the angle between the rotation axis of the rotating cylinder 320 and the vertical direction can be 30°, 40°, 45°, 50°, 60° or other angles. The setting of the angle between the rotation axis of the rotating cylinder 320 and the vertical direction facilitates the discharge of the separation liquid after the secondary separation, and can improve the working efficiency.

[0086] In some embodiments, the bacterial fermentation liquid separation and extraction device 10 further comprises a control mechanism. The control mechanism is electrically connected with the stirring component 220, the weight detection component 230, the pressure detection component 240 and the rotation driving component 340.

[0087] In some embodiments, the control mechanism can be a PLC programmable logic controller or a computer. The control mechanism can be programmed according to actual needs to realize the automatic operation or shutdown of the above-mentioned stirring component 220, weight detection component 230, pressure detection component 240 and rotation driving component 340 according to the set program.

[0088] In some embodiments, the bacterial fermentation liquid separation and extraction device 10 further comprises an alarm mechanism. The alarm mechanism is signal connected with the pressure detection component 240, and works when the pressure value P in the separation cavity 101 detected by the pressure detection component 240 is greater than the preset pressure value P1. The alarm mechanism is not shown in the drawings. The pressure detection component 240 monitors the pressure value P in the separation cavity 101 in real time. When the pressure value P is within the normal value range, it indicates that the primary separation of the separation cavity 101 is stable. When the pressure value P abnormally changes, for example, exceeds the preset pressure value P1, it is judged that at least part of the filter core 211 in the separation cavity 101 is blocked, and the alarm mechanism works. At this time, the backwashing port 105 needs to be opened and backwashing is performed to clean the attachments on the surface of the filter core 211 until the pressure value P is within the normal value range, and the alarm is removed.

[0089] In some embodiments, the alarm mechanism includes an alarm lamp, a buzzer and a vibrator, etc. The alarm mechanism can alarm through vision, hearing and touch to remind the operator.

[0090] The control mechanism analyzes the pressure value P data through real-time monitoring of the pressure detection component 240, judges whether the filter element 211 is blocked according to whether the pressure value P changes abnormally and the preset pressure value P1; analyzes the data of the weight detection component 230, calculates the density p of the liquid in the separation tank body 100 in real time, analyzes the process of the first-stage separation, and compares it with the set critical density p0; when the density p reaches the set critical density p0, the pneumatic valve 350 is opened, and the concentrated fermentation broth treated in the separation tank body 100 is transferred to the secondary separation mechanism 300 through the pipette port 104; the rotation speed of the stirring driver 224 is adjusted in real time through the analysis of the treatment process in the separation cavity 101, so that the bacteria and solid metabolites in the fermentation broth can be uniformly distributed in the separation cavity 101 at each stage of the treatment process; the treatment time of the secondary separation mechanism 300 is controlled by controlling the rotary driving component 340 of the secondary separation mechanism 300, and the secondary separation is completed.

[0091] An embodiment of the present application also provides a bacterial fermentation broth separation and extraction method.

[0092] It should be noted that, unless otherwise specified, each reaction step can be performed in the order described herein, or can not be performed in the order described herein. For example, each reaction step can include other steps, and the order of the reaction steps can be appropriately changed. This can be determined by a person skilled in the art based on conventional knowledge and experience. Preferably, the reaction method herein is performed sequentially.

[0093] A bacterial fermentation broth separation and extraction method using the bacterial fermentation broth separation and extraction device 10 described above, comprising the following steps:

[0094] The bacterial fermentation broth is placed in the separation cavity 101 of the separation tank body 100, the weight detection component 230 is controlled to detect the weight m of the bacterial fermentation broth in the separation tank body 100 in real time, and the pressure detection component 240 is controlled to detect the pressure in the separation cavity 101 in real time and obtain the pressure value P.

[0095] The stirring component 220 is controlled to stir the bacterial fermentation broth in the separation cavity 101, and the rotation speed of the stirring driver 224 is 200 rpm to 500 rpm. Under the continuous stirring action of the stirring component 220, the bacterial fermentation broth uniformly distributed in the separation cavity 101 forms a filter cake layer on the surface of the filter element 211 of the filter component 210, and the filtrate containing bacteria passes through the filter cake layer and is discharged out of the separation tank body 100 after the first-stage separation of the filter component 210.

[0096] The density of the liquid in the separation chamber 101 is calculated according to the weight m of the bacterial fermentation liquid in the separation tank 100 detected by the weight detection component 230, and the pressure value P in the separation chamber 101 detected by the pressure detection component 240, to determine whether the primary separation is completed. The density calculation formula is: p = m / V, wherein p represents the density of the liquid in the separation chamber 101, m represents the mass (weight) of the bacterial fermentation liquid in the separation tank 100, and V represents the volume of the bacterial fermentation liquid added into the separation chamber 101.

[0097] When it is determined that the primary separation is completed, the concentrated fermentation liquid in the separation chamber 101 is controlled to enter the rotating cylinder 320, and the rotating cylinder 320 is controlled to rotate, so as to realize the secondary separation of the concentrated fermentation liquid to obtain the solid metabolites and the separation liquid containing the bacterial bodies. The separated solid metabolites remain in the rotating cylinder 320, and the separation liquid containing the bacterial bodies is discharged to the separation cylinder 310 through the separation hole of the rotating cylinder 320, and can be discharged through the discharge outlet of the separation cylinder 310.

[0098] In some embodiments, when the pressure value P in the separation chamber 101 detected by the pressure detection component 240 in real time is greater than or equal to a preset pressure value P1, it indicates that the filter core 211 of the filter component 210 in the separation chamber 101 is blocked, and the filter core 211 of the filter component 210 is backwashed by inputting the backwashing liquid through the backwashing port 105 on the separation tank 100 until the pressure value P in the separation chamber 101 detected by the pressure detection component 240 is less than a preset pressure value P2.

[0099] In some embodiments, when the pressure value P is greater than or equal to a preset pressure value P1, it indicates that the filter core of the filter component is blocked, and the alarm mechanism is controlled to work.

[0100] In some embodiments, the liquid return pipeline 330 is controlled to guide the separation liquid containing the bacterial bodies after the secondary separation, and the separation liquid containing the bacterial bodies after the primary separation is combined.

[0101] In some embodiments, the thickness of the filter cake layer is controlled to be 0.4mm-1.6mm.

[0102] In some embodiments, when it is determined whether the primary separation is completed, the following steps are included: the density p of the liquid in the separation chamber 101 is calculated according to the weight m of the bacterial fermentation liquid in the separation tank 100 detected by the weight detection component 230, and the relationship between the density p and the critical density p0 is determined, when p is greater than or equal to p0 and the pressure value P is less than a preset pressure value P0, it indicates that the primary separation is completed, and the concentrated fermentation liquid in the separation chamber 101 is controlled to enter the rotating cylinder 320. It should be noted that when the density p is calculated, the weight m is the real-time weight of the bacterial fermentation liquid in the separation tank 100 at the time of judgment after the primary separation. Similarly, the pressure value P is the real-time pressure of the separation chamber 101 at the time of judgment after the primary separation.

[0103] In the bacterial fermentation liquor separation and extraction method, stirring control is added in the separation process, the filter cake layer with a thin core layer on the filter core 211 of the filter part 210 is realized as a solid layer, the thickness of the filter cake layer is controlled to be 0.4mm-1.6mm, the filtration and separation process is completed under the thin filter cake layer, most of the bacteria bodies can be recovered in the primary separation, the production cost is reduced, and the activity of the bacteria bodies is ensured not to be damaged. Further, the secondary separation mechanism 300 is used to perform secondary separation on the concentrated fermentation liquor after the primary separation, the solid metabolic product generated by fermentation is directly obtained, the time of transferring the fermentation liquor between devices is reduced, and the separation and extraction efficiency is improved.

[0104] Embodiment 1

[0105] The embodiment provides a bacterial fermentation liquor separation and extraction device 10.

[0106] Please refer to Figure 1 and Figure 2 The bacterial fermentation liquor separation and extraction device 10 of the embodiment includes a separation tank body 100, a primary separation mechanism 200, a secondary separation mechanism 300, an alarm mechanism and a control mechanism.

[0107] The bottom of the separation tank body 100 is connected with a support 107, and the separation tank body 100 is installed on a plurality of supports 107. The separation tank body 100 has a separation cavity 101. The separation tank body 100 has a liquid inlet 102, a liquid outlet 103, a pipetting port 104 and a backwashing port 105 which are communicated with the separation cavity 101. The position of the liquid outlet 103 is higher than that of the liquid inlet 102 along the height direction of the separation tank body 100. The filter part 210 is communicated with the liquid outlet 103 for discharging the bacteria bodies after the primary separation, and the separation cylinder 310 is communicated with the separation cavity 101 through the pipetting port 104.

[0108] The primary separation mechanism 200 includes a filtering component 210, an agitating component 220, a weight detecting component 230, and a pressure detecting component 240. The filtering component 210 includes filter cartridges 211 and a liquid collecting pipe 212, the filter cartridges 211 are arranged in the separation chamber 101, the number of the filter cartridges 211 is plural, the plural filter cartridges 211 are distributed at intervals, and the filter cartridges 211 have filtering holes. The filter cartridges 211 extend from the top of the separation chamber 101 to the bottom of the separation chamber 101, and the filter cartridges 211 have an interval with the bottom of the separation chamber 101. The filter cartridges 211 have a plurality of filtering holes, the diameter of the filtering holes is slightly larger than the diameter of the bacteria and smaller than the diameter of the solid metabolites. The liquid collecting pipe 212 is close to the top of the separation chamber 101, the filter cartridges 211 are connected to the liquid collecting pipe 212 and discharge the bacteria after the primary separation through the liquid collecting pipe 212. The backwashing port 105 is communicated with the liquid collecting pipe 212, and the backwashing port 105 is used to backwash the blocked filter cartridges 211 by the backwashing liquid.

[0109] The agitating component 220 is connected to the separation tank body 100 for agitating the bacteria fermentation liquid in the separation chamber 101. The agitating component 220 includes an agitating shaft 221, a sealing member 222, an agitating paddle 223, an agitating driver 224, a driver support 225, and a coupling 226. The agitating shaft 221 extends into the separation chamber 101 and extends to the outside of the separation tank body 100. The agitating shaft 221 is sealed with the separation tank body by the sealing member 222. The agitating paddle 223 is located in the separation chamber 101 and is connected to the agitating shaft 221. The agitating paddle 223 is arranged below the filter cartridges 211 in the separation chamber 101. The driver support 225 is connected to the top of the separation tank body 100, the agitating driver 224 is installed on the driver support 225, and the agitating driver 224 is connected to the agitating shaft 221 through the coupling 226.

[0110] The weight detecting component 230 is installed on the support 107 and connected to the separation tank body 100 for detecting the weight m of the bacteria fermentation liquid in the separation tank body 100. The pressure detecting component 240 is connected to the separation tank body 100 for detecting the pressure in the separation chamber 101.

[0111] The secondary separation mechanism 300 comprises a separation cylinder 310, a rotating cylinder 320, a liquid return pipeline 330 and a rotating driving component 340. The separation cylinder 310 is communicated with the separation cavity 101, and a pneumatic valve 350 is arranged on the communication pipeline between the separation cylinder 310 and the separation tank body 100. The communication pipeline between the separation cylinder 310 and the separation tank body 100 is arranged in a vertical direction. The separation cylinder 310 and the rotating cylinder 320 are both arranged in an inclined manner, and the rotating shaft of the rotating cylinder 320 has an angle of 45° with the vertical direction. The rotating cylinder 320 has a plurality of separation holes, and the diameter of the separation holes is greater than the diameter of the bacteria and less than the diameter of the solid metabolites. The rotating cylinder 320 is rotatably arranged in the separation cylinder 310, and the rotating driving component 340 is connected to the rotating cylinder 320 for driving the rotating cylinder 320 to rotate. The liquid return pipeline 330 is communicated with the separation cylinder 310 from the lowest point of the separation cylinder 310, and is used for draining the separation liquid containing bacteria.

[0112] The control mechanism is electrically connected with the stirring component 220, the weight detection component 230, the pressure detection component 240, the pneumatic valve 350, the rotating driving component 340 and the alarm mechanism.

[0113] An embodiment of the present application also provides a bacteria fermentation liquid separation and extraction method, which is used for the separation and extraction of sophorolipid fermentation liquid. The bacteria diameter ranges from 0.6 μm to 3.2 μm, and the diameter of the solid metabolites ranges from >5 μm.

[0114] A bacteria fermentation liquid separation and extraction method is adopted in the bacteria fermentation liquid separation and extraction device 10, and comprises the following steps.

[0115] S1, the bacteria fermentation liquid is placed in the separation cavity 101 of the separation tank body 100 through the liquid inlet 102, and then the liquid inlet 102 is closed. The control mechanism controls the weight detection component 230 to detect the weight m of the bacteria fermentation liquid in the separation tank body 100 in real time, and controls the pressure detection component 240 to detect the pressure in the separation cavity 101 to obtain the pressure value P.

[0116] S2, the control mechanism controls the stirring component 220 to stir the bacteria fermentation liquid in the separation cavity 101. The rotating speed of the stirring driver 224 is controlled to be 300 revolutions per minute. Under the continuous stirring action of the stirring component 220, the bacteria fermentation liquid uniformly distributed in the separation cavity 101 forms a filter cake layer with a thickness of 1.2 mm on the surface of the filter element 211 of the filter component 210. The filtrate containing bacteria passes through the filter cake layer and is discharged out of the separation tank body 100 after the first separation of the filter component 210.

[0117] S3, the initial liquid density ρ=1.2x10 3kg / m 3 and the pressure detection component 240 detects the initial pressure value P = 0.05 MPa in the separation chamber 101, as the separation and extraction process proceeds, the liquid density p and the critical density p0 = 1.42 x 10 3 kg / m 3 When p > p0 and the pressure value P > the preset pressure value P0 = 0.2 MPa, it indicates that the primary separation is completed, the control mechanism opens the pneumatic valve 350 to open, and controls the concentrated fermentation liquid in the separation chamber 101 to enter the rotating cylinder 320. When the pressure value P detected by the pressure detection component 240 in the separation chamber 101 > the preset pressure value P1 = 0.4 MPa, it indicates that the filter core 211 of the filter component 210 in the separation chamber 101 is blocked, at this time, the control mechanism controls the stirring component 220 to stop stirring, and inputs the backwash liquid through the backwash port 105 on the separation tank body 100 to backwash the filter core 211 of the filter component 210, until the pressure value P detected by the pressure detection component 240 in the separation chamber 101 in real time < the preset pressure value P2 = 0.02 MPa.

[0118] S4, when p > p0 and the pressure value P < the preset pressure value P0, it is judged that the primary separation is completed, at this time the control mechanism opens the pneumatic valve 350 to open, and realizes the concentrated fermentation liquid in the separation chamber 101 to enter the rotating cylinder 320.

[0119] S5, the control mechanism controls the rotating driving component 340 to drive the rotating cylinder 320 to rotate at a speed of 1500 revolutions / min, to realize the separation of the separation liquid containing part of the bacteria in the concentrated fermentation liquid. The return liquid pipeline 330 is opened to drain the separation liquid containing bacteria after the secondary separation. The return liquid pipeline 330 drains the treated separation liquid containing bacteria and the filtrate of the bacteria after the primary separation. The separation chamber 101 is opened to obtain the solid metabolite.

[0120] S6, the activity of the separated bacteria is detected. The separated bacteria are added to the newly cultured sophorolipid fermentation liquid, and sophorolipid can be normally produced. After the same fermentation time of 150 h, the content of sophorolipid in the fermentation liquid is measured, and the specific comparison data is: the content of sophorolipid in the initial fermentation liquid is about 408 g / L, and the content of sophorolipid in the fermentation liquid after the secondary fermentation of the separated bacteria is about 376 g / L, which can prove that the separated bacteria have strong activity, meet the needs of actual production, and can realize the reuse of the bacteria.

[0121] In summary, the bacterial fermentation liquor separation and extraction device 10 can realize efficient extraction and recovery of the bacteria and rapid separation of the solid metabolites, the recovered bacteria can be reused, and the production cost is reduced.

[0122] In the above embodiments, the description of each embodiment has its own focus, and the parts not described in detail in a certain embodiment can be referred to the relevant description of other embodiments.

[0123] The technical features of the above-described embodiments can be combined arbitrarily, and in order to make the description concise, not all possible combinations of the technical features in the above embodiments are described, however, as long as the combinations of the technical features do not exist contradictory, they should be considered as the scope of the present application.

[0124] The above-described embodiments only express several implementation manners of the present application, the description is more specific and detailed, but it should not be understood as the limitation of the patent scope of the present application. It should be pointed out that for ordinary skilled in the art, without departing from the concept of the present application, a number of modifications and improvements can be made, which all belong to the protection scope of the present application. Therefore, the protection scope of the present application patent should be subject to the appended claims.

Claims

1. A bacterial fermentation broth separation and extraction device, characterized in that, It includes a separation tank, a primary separation mechanism, and a secondary separation mechanism; the separation tank has a sealable separation chamber; The primary separation mechanism includes a filtration component, a stirring component, a weight detection component, and a pressure detection component. The filtration component is disposed in the separation chamber for primary separation of the bacterial fermentation broth to obtain bacterial cells and concentrated fermentation broth. The stirring component is connected to the separation tank for stirring the bacterial fermentation broth in the separation chamber. The weight detection component is used to detect the weight of the bacterial fermentation broth in the separation tank. The pressure detection component is used to detect the pressure in the separation chamber. The secondary separation mechanism includes a separation cylinder and a rotating cylinder. The rotating cylinder is connected to the separation chamber for collecting the concentrated fermentation broth after the primary separation. The rotating cylinder has several separation holes with a pore size of 3.1 μm to 3.5 μm. The pore size of the separation holes is larger than the diameter of the bacterial cells and smaller than the diameter of the solid metabolites. The rotating cylinder is rotatably disposed inside the separation cylinder, and the rotating cylinder can rotate to achieve secondary separation of the concentrated fermentation broth. The connecting pipe between the separating cylinder and the separating tank is arranged vertically, and both the separating cylinder and the rotating cylinder are arranged at an angle, with the rotation axis of the rotating cylinder forming an angle with the vertical direction.

2. The bacterial fermentation broth separation and extraction device according to claim 1, characterized in that, The separation tank also has an inlet, an outlet, and a transfer port that communicate with the separation chamber. The outlet is positioned higher than the inlet along the height of the separation tank. The filter component is connected to the outlet, and the separation cylinder is connected to the separation chamber through the transfer port.

3. The bacterial fermentation broth separation and extraction device according to claim 1, characterized in that, The filtration component includes a filter element disposed in the separation chamber and a liquid collection pipe. The filter element has a plurality of filter holes, the diameter of which is larger than the diameter of the bacterial cells and smaller than the diameter of the solid metabolites. The filter element is connected to the liquid collection pipe and can pass through the liquid collection pipe.

4. The bacterial fermentation broth separation and extraction device according to claim 3, characterized in that, The bacterial fermentation broth separation and extraction device also meets at least one of the following conditions: (1) The number of filter elements is multiple, and the multiple filter elements are distributed at intervals in the separation chamber; (2) The filter element extends from the top of the separation tank to the bottom of the separation tank, and there is a gap between the filter element and the bottom of the separation tank; (3) The liquid collection pipe is located near the top of the separation tank; (4) The separation tank also has a backwash port, which is connected to the liquid collection pipe. The backwash port is used to connect backwash liquid to backwash the clogged filter element.

5. The bacterial fermentation broth separation and extraction apparatus according to any one of claims 1 to 4, characterized in that, The stirring component includes a stirring shaft, a seal, a stirring paddle, and a stirring driver. A portion of the stirring shaft extends into the separation chamber and another portion extends to the outside of the separation tank. The stirring shaft is dynamically sealed to the separation tank through the seal. The stirring paddle is located inside the separation chamber and connected to the stirring shaft. The stirring driver is installed in the separation tank and connected to the stirring shaft.

6. The bacterial fermentation broth separation and extraction device according to claim 5, characterized in that, The bacterial fermentation broth separation and extraction device also meets at least one of the following conditions: (1) The stirring paddle is located below the filter element and close to the bottom of the separation chamber; (2) The stirring component further includes a driver support, the driver support is connected to the top of the separation tank, and the stirring driver is installed on the driver support; (3) The stirring component also includes a coupling, and the stirring drive is connected to the stirring shaft through the coupling.

7. The bacterial fermentation broth separation and extraction apparatus according to any one of claims 1 to 4, 6, characterized in that, The bacterial fermentation broth separation and extraction device also meets at least one of the following conditions: (1) The bottom of the separation tank is connected to a support member, the separation tank is installed on a plurality of the support members, and the weight detection component is installed on the support member and connected to the separation tank; (2) A pneumatic valve is installed on the connecting pipeline between the separation cylinder and the separation tank; (3) The secondary separation mechanism further includes a return liquid pipeline, which is connected to the separation cylinder to guide the separation liquid containing bacteria after the secondary separation. (4) The secondary separation mechanism further includes a rotary drive component, which is connected to the rotating cylinder to drive the rotating cylinder to rotate; (5) The bacterial fermentation broth separation and extraction device further includes an alarm mechanism, which is connected to the pressure detection component. When the pressure value detected by the pressure detection component in the separation chamber is greater than the preset pressure value, the alarm mechanism is activated.

8. A method for separating and extracting bacterial fermentation broth, characterized in that, The bacterial fermentation broth separation and extraction apparatus according to any one of claims 1 to 7 includes the following steps: The bacterial fermentation broth is placed in the separation chamber of the separation tank. The weight detection component controls the weight m of the bacterial fermentation broth in the separation tank in real time, and the pressure detection component controls the pressure value P in the separation chamber in real time. The stirring component is controlled to stir the bacterial fermentation broth in the separation chamber. The bacterial fermentation broth forms a filter cake layer on the surface of the filter element of the filtration component. The filtrate containing bacteria passes through the filter cake layer and is discharged from the separation tank after primary separation by the filtration component. Based on the pressure value P and the liquid density ρ in the separation chamber calculated from the weight m of the bacterial fermentation liquid in the separation tank, it is determined whether the first-stage separation is complete. After the primary separation is completed, the concentrated fermentation broth in the separation chamber is controlled to enter the rotating cylinder, and the rotating cylinder is controlled to rotate to achieve secondary separation of the concentrated fermentation broth to obtain solid metabolites and a separation broth containing bacteria.

9. The method for separating and extracting bacterial fermentation broth according to claim 8, characterized in that, The bacterial fermentation broth separation and extraction method further includes at least one of the following steps: (1) When the pressure value P ≥ the preset pressure value P1, it indicates that the filter element of the filter component is blocked. Backwash liquid is introduced through the backwash port on the separation tank to backwash the filter element of the filter component until the pressure value P < the preset pressure value P2. (2) When the pressure value P ≥ the preset pressure value P1, it indicates that the filter element of the filter component is blocked, and the alarm mechanism is activated. (3) Control the return liquid pipeline to drain the separation liquid containing bacteria after secondary separation and combine it with the filtrate containing bacteria after primary separation; (4) The thickness of the filter cake layer is controlled to be 0.4 mm to 1.6 mm.

10. The method for separating and extracting bacterial fermentation broth according to claim 8 or 9, characterized in that, When determining whether the first-stage separation is complete, the following steps are included: Calculate the liquid density ρ in the separation chamber based on the weight m of the bacterial fermentation broth in the separation tank, determine the relationship between the liquid density ρ and the critical density ρ0, and indicate that the first-stage separation is complete when ρ≥ρ0 and the pressure value P<preset pressure value P0.

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