Panda expected date of date and true and false pregnancy judgment method

Abstract

The invention relates to the technical field of panda breeding, and discloses a panda expected date of confinement and true and false pregnancy judgment method which comprises the following steps: after panda mating, collecting and detecting the progesterone value of a urine sample in a gestation period every day; counting the excrement amount of the giant pandas in the gestation period; taking the current day of the progesterone peak value of the farrowing panda as 0 day, taking the number of days as an abscissa, and taking the progesterone value and the excrement amount of a urine sample in the gestation period of the panda as an ordinate to construct a change mode pattern of the progesterone value and the excrement amount of the panda; and the true and false pregnancy conditions and the expected date of delivery of the panda are judged by observing the change mode pattern. According to the method, the true and false pregnancy conditions of the pandas can be judged by observing the food reduction and excrement amount change conditions of the pandas after the point C, and the expected date of birth of the true pregnancy pandas is predicted according to the time after the progesterone value peak point B. Therefore, management personnel can conveniently strengthen gestation period management on the pandas in the embryo implantation period; the method effectively prevents early embryo loss of pandas, improves the pregnancy rate of pandas, and prevents early abortion of fetuses.

Description

Technical Field

[0001] The invention relates to the technical field of giant panda breeding, and in particular to a method for judging the expected delivery date and true or false pregnancy of a giant panda. Background Art

[0002] Giant pandas are rare protected animals in China. They are seasonal single-estrus animals. They usually estrus once a year in spring, which lasts one to two weeks, and give birth in autumn. The embryonic development of giant pandas is typical of dormancy. After fertilization, the egg cell floats in the uterus and does not immediately implant into the uterine wall. Due to the different lengths of the blastocyst free period, the length of the giant panda's gestation period varies greatly, ranging from 89 to 186 days. Giant pandas also have pseudopregnancy, which has extremely similar progesterone changes and behavioral performance to true pregnant giant pandas. Even in the late pregnancy, it is difficult to judge whether the mother giant panda is pregnant based on the changes in the mother's weight and morphology. There is also a phenomenon of delayed implantation after mating of giant pandas. There is almost no change in the months before delivery, except for a decrease in food intake when delivery is imminent.

[0003] At present, most of the giant panda reproductive hormone detection technology is used to determine whether the giant panda is pregnant or the due date is approaching. It is necessary to collect fresh morning urine from the female giant panda every day after mating until one week after delivery or the end of false pregnancy, and count the total amount of giant panda feces every day, use ELISA detection method to detect the progesterone level in the urine sample, and then use Microsoft Excel to organize and analyze the data. However, this method cannot directly judge the due date of the giant panda after mating. It is necessary to combine the progesterone data and the change in feces volume to analyze and determine whether the giant panda is pregnant and predict the due date. It can be seen that the existing technology is particularly difficult to judge the due date of the giant panda and the true or false pregnancy of the giant panda, which is easy to cause the loss of the early embryo of the giant panda, reduce the pregnancy rate of the giant panda, and the early miscarriage of the giant panda fetus, which is not conducive to the health care of the pregnant giant panda. In view of the above problems, it is urgent for those skilled in the art to propose a method for judging the due date of the giant panda and the true or false pregnancy of the giant panda that can predict the due date of the giant panda, judge the true or false pregnancy of the giant panda, improve the pregnancy rate, and prevent the loss of the early embryo of the giant panda and the early miscarriage of the fetus. Summary of the invention

[0004] The present invention aims at the above technical problems and provides a method for judging the expected date of delivery and true or false pregnancy of giant pandas, which solves the problems in the prior art that the expected date of delivery and true or false pregnancy of giant pandas are difficult to judge, the early embryos of giant pandas are easy to lose, the pregnancy rate of giant pandas is low, and the early fetuses are easy to miscarry.

[0005] In order to achieve the above object, the technical solution adopted by the present invention is as follows: A method for judging the expected date of delivery and true or false pregnancy of a giant panda of the present invention comprises the following steps: S1: After the giant pandas are mated, morning urine is collected once a day and the progesterone level of the giant pandas’ urine during pregnancy is tested; S2: Count the amount of feces during the pregnancy of giant pandas to monitor their appetite; S3: The peak day of the giant panda's progesterone level during childbirth was recorded as day 0, the number of days was used as the horizontal axis, and the urine progesterone level and feces volume of the giant panda during pregnancy were used as the vertical axis to construct a graph showing the change pattern of the giant panda's progesterone level and feces volume; S4: The peak value of progesterone is recorded as point B, the progesterone value when the feces volume begins to decrease is recorded as point A, the progesterone value when the feces volume of pseudo-pregnant giant pandas begins to increase is recorded as point C, and the progesterone value when the giant panda gives birth is recorded as point D; S5: Combine the values ​​of points A, B, C, and D to determine whether the giant panda is truly pregnant or not, as well as the expected date of birth.

[0006] Furthermore, the method for judging the true or false pregnancy of the giant panda in step S5 is as follows: after point A, the giant panda begins to eat less and the amount of feces begins to decrease. If after point C, the giant panda's appetite gradually recovers and the amount of feces begins to increase, it is a false pregnancy; if after point C, the giant panda's feces volume is still at a low level, it is a true pregnancy.

[0007] Furthermore, there are two types of changes in the amount of food and feces of pregnant giant pandas: one is that after reducing food intake, the appetite is very low, and the pandas are not interested in the food they usually like, and they only eat a small amount occasionally. The amount of feces remains at a low level with small fluctuations. The other is that the pandas will still eat after reducing food intake, but their appetite is unstable, and the total amount of food eaten per day is still significantly lower than normal. The amount of feces will fluctuate within a small range, but the amount of feces will not continue to increase.

[0008] Furthermore, the pseudo-pregnant panda's appetite will gradually recover and the amount of feces will gradually increase after point C. By observing the panda's reduced food intake and changes in feces after point C, the true or false pregnancy of the panda can be determined.

[0009] Furthermore, the progesterone value of the truly pregnant giant panda gradually increased 17.8±0.9 days before the peak value B of the progesterone value; the progesterone value of the falsely pregnant giant panda gradually increased 16.7±1.4 days before the peak value B of the progesterone value.

[0010] Furthermore, the method for determining the expected date of delivery of the giant panda in step S5 is as follows: when the giant panda is truly pregnant, the panda gives birth 22.0±0.71 days after the peak value of progesterone value B. By observing the peak value of progesterone value B, the expected date of delivery of the giant panda can be predicted.

[0011] Compared with the prior art, the present invention has the following beneficial effects: the present invention can judge the true or false pregnancy of giant pandas by observing the pandas' reduced food intake and changes in feces volume after point C, and then predict the expected date of delivery of truly pregnant giant pandas by the time after the peak of progesterone value at point B. The prediction of the expected date of delivery of giant pandas helps managers understand the pregnancy stage of giant pandas, and facilitates managers to strengthen pregnancy management of giant pandas in the embryo implantation period; thus, it can effectively prevent the loss of early embryos of giant pandas, improve the pregnancy rate of giant pandas, and prevent early miscarriage of fetuses. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Attached Figure 1 This is a graph showing the change pattern of progesterone value and feces volume of giant pandas in this embodiment; Attached Figure 2 The changes of progesterone and feces volume of giant pandas giving birth in this example Figure 1 ; Attached Figure 3 The changes of progesterone and feces volume of giant pandas giving birth in this example Figure 2 ; Attached Figure 4 This is a graph showing the changes in progesterone and feces volume in pseudo-pregnant giant pandas in this example. DETAILED DESCRIPTION

[0013] In order to make the purpose and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention. Example

[0014] This example uses the results of 28 breedings of 11 giant pandas that participated in breeding at the Qinling Giant Panda Research Center from 2019 to 2023 for statistical verification, with a total of 18 successful births and a birth rate of 64.3%.

[0015] See attached Figure 1-4 , a method for judging the expected date of delivery and true or false pregnancy of a giant panda in this embodiment comprises the following steps: S1: After the giant pandas are mated, morning urine is collected once a day and the progesterone value in the morning urine sample of the giant pandas during pregnancy is tested; S2: Count the amount of feces during the pregnancy of giant pandas to monitor their appetite; S3: The peak day of the giant panda's progesterone level during childbirth was recorded as day 0, the number of days was used as the horizontal axis, and the urine progesterone level and feces volume of the giant panda during pregnancy were used as the vertical axis to construct a graph showing the change pattern of the giant panda's progesterone level and feces volume. Figure 1 , where I 0 is the progesterone curve, I 1 is the pseudo-pregnancy stool volume curve, I 2 This is the true pregnancy stool volume curve; S4: The peak value of progesterone in the pregnant giant panda was recorded as point B, the progesterone value when the feces volume began to decrease was recorded as point A, the progesterone value when the feces volume of the pseudo-pregnant giant panda began to increase was recorded as point C, and the progesterone value of the pregnant giant panda at the time of giving birth was recorded as point D; S5: Determine the true or false pregnancy and expected date of giant pandas by combining the values ​​of points A, B, C, and D respectively: The specific method for determining the true or false pregnancy of giant pandas is as follows: After point A, the giant pandas begin to eat less and the amount of feces begins to decrease. If the appetite of the giant pandas gradually recovers after point C and the amount of feces begins to increase, it is a false pregnancy; if the amount of feces of the giant pandas is still at a low level after point C, it is a true pregnancy; when the giant panda is truly pregnant, it will give birth 22.0±0.71 days after the peak value of progesterone B. The true or false pregnancy of the giant panda can be determined by observing the reduction of food intake and the changes in feces after point C, and the expected date of birth of the giant panda can be predicted by observing the peak value of progesterone B.

[0016] Among them, the progesterone level of truly pregnant giant pandas gradually increased 17.8±0.9 days before the peak value B; the progesterone level of falsely pregnant giant pandas gradually increased 16.7±1.4 days before the peak value B.

[0017] The progesterone values ​​and feces volume of giant pandas after mating were statistically analyzed. The statistical results are shown in Table 1: Table 1 Progesterone levels and feces volume data of giant pandas after mating

[0018]

[0019]

[0020]

[0021] According to the table above, there are two types of changes in the amount of food and feces of pregnant giant pandas: one is that after reducing food intake, the appetite is very low, and they are not interested in the food they usually like. They eat a small amount occasionally, and the amount of feces is always at a low level with little fluctuation. See the attached Figure 2 The other is that the patient still eats after reducing food intake, but the appetite is unstable, the total amount of food consumed per day is still significantly lower than normal, and the amount of stool fluctuates within a small range, but the amount of stool does not continue to increase. See the attached Figure 3 ; The appetite of pseudo-pregnant giant pandas will gradually recover and the amount of feces will gradually increase after point C. Figure 4 .

[0022] By the attached Figure 1-4It can be seen that the method of this embodiment can determine the true or false pregnancy of the giant panda by observing the panda's reduced food intake and changes in the amount of feces after point C, and then predict the expected date of birth of the truly pregnant giant panda by the time after the peak of the progesterone value at point B. The prediction of the expected date of birth of the giant panda helps the management personnel understand the pregnancy stage of the giant panda, and facilitates the management personnel to strengthen the pregnancy management of the giant panda in the embryo implantation period; thus, it can effectively prevent the loss of early embryos of giant pandas, improve the pregnancy rate of giant pandas, and prevent early miscarriage of the fetus.

[0023] The above description is only a preferred embodiment of the present application and an explanation of the technical principles used. Those skilled in the art should understand that the scope of the invention involved in the present application is not limited to the technical solutions formed by the specific combination of the above technical features, but should also cover other technical solutions formed by any combination of the above technical features or their equivalent features without departing from the inventive concept. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for determining the expected date of delivery and true or false pregnancy of a giant panda, characterized by: The method comprises the following steps: S1: After the giant pandas are mated, morning urine is collected once a day and the progesterone level of the giant pandas’ urine during pregnancy is tested; S2: Count the amount of feces during the giant panda's pregnancy; S3: The peak day of the giant panda's progesterone level during childbirth was recorded as day 0, the number of days was used as the horizontal axis, and the urine progesterone level and feces volume of the giant panda during pregnancy were used as the vertical axis to construct a graph showing the change pattern of the giant panda's progesterone level and feces volume; S4: The peak value of progesterone is recorded as point B, the progesterone value when the feces volume begins to decrease is recorded as point A, the progesterone value when the feces volume of pseudo-pregnant giant pandas begins to increase is recorded as point C, and the progesterone value when the giant panda gives birth is recorded as point D; S5: Combine the values ​​of points A, B, C, and D to determine whether the giant panda is truly pregnant or not, as well as the expected date of birth.

2. A method for determining the expected date of delivery and true or false pregnancy of a giant panda according to claim 1, characterized in that: The method for judging the true or false pregnancy of the giant panda in step S5 is as follows: after point A, the giant panda begins to eat less and the amount of feces begins to decrease. If after point C, the giant panda's appetite gradually recovers and the amount of feces begins to increase, it is a false pregnancy; if after point C, the giant panda's feces volume is still at a low level, it is a true pregnancy.

3. A method for determining the expected date of delivery and true or false pregnancy of a giant panda according to claim 2, characterized in that: There are two types of changes in the amount of food and feces of pregnant giant pandas: one is that after reducing food intake, the appetite is very low, and the panda is not interested in the food it usually likes. It only eats a small amount occasionally, and the amount of feces remains at a low level with small fluctuations. The other is that the panda will still eat after reducing food intake, but its appetite is unstable, and the total amount of food eaten per day is still significantly lower than normal. The amount of feces will fluctuate within a small range, but the amount of feces will not continue to increase.

4. A method for determining the expected date of delivery and true or false pregnancy of a giant panda according to claim 2, characterized in that: The pseudo-pregnant giant panda will eat less and excrement volume will gradually increase after point C.

5. A method for determining the expected date of delivery and true or false pregnancy of a giant panda according to claim 2, characterized in that: The progesterone value of the truly pregnant giant panda gradually increased 17.8±0.9 days before the peak value B of the progesterone value; the progesterone value of the falsely pregnant giant panda gradually increased 16.7±1.4 days before the peak value B of the progesterone value.

6. A method for determining the expected date of delivery and true or false pregnancy of a giant panda according to claim 1, characterized in that: The method for determining the expected date of delivery of a giant panda in step S5 is as follows: when the giant panda is truly pregnant, it will give birth 22.0±0.71 days after the peak value B of the progesterone value.

Images