Use of n-acetyl-l-cysteine in improving drought tolerance in plants

Abstract

The application discloses application of N-acetyl-L-cysteine in improving drought resistance of plants and belongs to the technical field of plant cultivation.The application first proposes application of N-acetyl-L-cysteine (NAC) in improving drought resistance of Brassica napus L.And through experiments, it is proved that compared with a control group, the fresh weight, dry weight, root length and chlorophyll content of the Brassica napus L seedlings that are sprayed with 150 muM of N-acetyl-L-cysteine on leaves are improved, the leaf water loss rate is reduced, and the drought stress tolerance of the Brassica napus L is enhanced.This reflects that the drought stress tolerance of the Brassica napus L treated with N-acetyl-L-cysteine is obviously improved.Further experiments are conducted to obtain an optimal application concentration of 150 muM, so that the N-acetyl-L-cysteine can be optimally used in improving the drought stress tolerance of the Brassica napus L.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of plant cultivation, in particular to the application of N-acetyl-L-cysteine in improving the drought tolerance of plants. BACKGROUND

[0002] Global climate change intensification is one of the most severe challenges facing the world today. Global temperature rise, changes in precipitation patterns, and an increase in extreme weather events caused by climate change pose a significant threat to agricultural production. Among them, soil drought is particularly serious, which directly affects the growth of crops and thus affects food security and sustainable agricultural development. Soil drought refers to a severe lack of water in the soil, which cannot meet the needs of normal plant growth. This situation is increasingly common in many arid and semi-arid regions around the world, especially under the background of climate change, the frequency and intensity of drought events have increased. Crops will experience water stress under drought conditions, which will hinder physiological and biochemical processes, and thus affect their growth and yield. For example, drought stress usually leads to reduced photosynthesis, closed stomata, and decreased growth rate, thereby significantly reducing crop yield.

[0003] Brassica napus is an important oil crop widely used for producing edible oil and industrial raw materials. With the increasing global population and decreasing resources, it is particularly important to improve the yield and adaptability of oilseed rape. Due to the influence of climate change, drought has become one of the main environmental factors limiting the production of Brassica napus. Oilseed rape has relatively high water requirements, and drought will directly affect its growth and seed yield. Under drought conditions, the growth of oilseed rape plants will be hindered, and the flowering and seed setting periods may be advanced, which usually leads to a significant decrease in yield. In addition, drought can also affect the oil quality and quantity of oilseed rape seeds, reducing their economic value. In this context, the development and application of effective drought-resistant technologies and methods have become a research focus.

[0004] N-acetyl-L-cysteine (NAC) is a sulfur-containing amino acid that has important physiological functions in various organisms. Under drought conditions, a large amount of reactive oxygen species is produced in plants, which can damage cell structures and affect the normal physiological functions of plants. N-acetyl-L-cysteine can enhance the antioxidant system of plants, scavenge excess reactive oxygen species, and thus alleviate the damage of oxidative stress to plants. There is no related report on the use of N-acetyl-L-cysteine to improve the drought tolerance of plants. SUMMARY

[0005] The application provides application of N-acetyl-L-cysteine in improving drought resistance of plants, so as to solve the problems in the prior art.

[0006] To achieve the above object, the application provides the following scheme.

[0007] One of the technical schemes of the application is application of N-acetyl-L-cysteine in improving drought resistance of plants.

[0008] The second technical scheme of the application is application of N-acetyl-L-cysteine in preparing a product for improving drought resistance of plants.

[0009] The third technical scheme of the application is a product for improving drought resistance of plants, which comprises N-acetyl-L-cysteine.

[0010] The fourth technical scheme of the application is a method for improving drought resistance of plants, which comprises spraying a plant with a N-acetyl-L-cysteine solution or the product.

[0011] Based on the above technical scheme, the application has the following technical effects.

[0012] The application first proposes application of N-acetyl-L-cysteine (N-acetyl-L-cysteine, NAC) in improving drought resistance of Brassica napus L., and experiments prove that, compared with a control group, Brassica napus L. seedlings sprayed with 150 mu of N-acetyl-L-cysteine have improved fresh weight, dry weight, root length, chlorophyll content under drought stress conditions, and reduced leaf water loss rate, and the drought stress tolerance of the Brassica napus L. seedlings is improved. This reflects that the drought stress tolerance of the Brassica napus L. seedlings treated with N-acetyl-L-cysteine is obviously improved. Further experiments prove that the optimal spraying concentration of N-acetyl-L-cysteine is 150 mu, so that N-acetyl-L-cysteine can be optimally used in improving drought stress tolerance of Brassica napus L. BRIEF DESCRIPTION OF DRAWINGS

[0013] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed in the embodiments. Obviously, the drawings described below only illustrate some of the embodiments of the present application, and for those skilled in the art, other drawings can also be obtained from these drawings without creative effort.

[0014] Figure 1 Fresh weight of Brassica napus treated with N-acetyl-L-cysteine solution for 9 days under control and drought conditions.

[0015] Figure 2 Fresh weight of Brassica napus treated with N-acetyl-L-cysteine solution for 9 days under control and drought conditions.

[0016] Figure 3 Fresh weight of Brassica napus treated with N-acetyl-L-cysteine solution for 9 days under control and drought conditions.

[0017] Figure 4 Fresh weight of Brassica napus treated with N-acetyl-L-cysteine solution for 9 days under control and drought conditions.

[0018] Figure 5 Fresh weight of Brassica napus treated with N-acetyl-L-cysteine solution for 9 days under control and drought conditions.

[0019] Figure 6 Fresh weight of Brassica napus treated with N-acetyl-L-cysteine solution for 9 days under control and drought conditions. DETAILED DESCRIPTION

[0020] The various illustrative embodiments of the present application will now be described in detail below. The detailed description is made with reference to the drawings, wherein like reference numerals indicate like elements throughout the several views. The detailed description is made with reference to the drawings, wherein like reference numerals indicate like elements throughout the several views. The detailed description is made with reference to the drawings, wherein like reference numerals indicate like elements throughout the several views.

[0021] It should be understood that the terms used in the present application merely describe particular embodiments and are not intended to limit the present application. In addition, for numerical ranges in the present application, it should be understood that each intermediate value between the upper limit and the lower limit of the range is specifically disclosed. Each smaller range between any stated value or stated range and any other stated value or stated range is also included in the present application. The upper limit and the lower limit of these smaller ranges can be included or excluded independently.

[0022] Unless otherwise indicated, all technical and scientific terms used herein have the same meaning as those commonly understood by one of ordinary skill in the art to which this application pertains. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present application, the preferred methods and materials are described. All publications mentioned in this specification are herein incorporated by reference to disclose and describe the methods and / or materials in connection with which the publications are cited. In case of conflict between the content of the specification and that of any incorporated literature, the content of the specification controls.

[0023] Many modifications and variations of this application of the application can be made without departing from its spirit or scope, which will be apparent to those skilled in the art. Other embodiments of the application will be apparent to those skilled in the art from consideration of the specification and practice of the application disclosed herein. The specification and examples given are exemplary only.

[0024] As used herein, the terms "comprise", "comprising", "include", "including", "have", "having" and the like are open-ended and do not exclude additional, unrecited elements or method steps.

[0025] The technical solutions described in the present application are conventional solutions in the art, and the reagents or raw materials used are commercially available or have been disclosed, unless otherwise specified.

[0026] The embodiments of the present application provide application of N-acetyl-L-cysteine in improving drought tolerance of plants.

[0027] The chemical structural formula of N-acetyl-L-cysteine is as follows:

[0028]

[0029] In some specific embodiments, the plant comprises Brassica napus.

[0030] The embodiments of the present application also provide application of N-acetyl-L-cysteine in preparing a product for improving drought tolerance of plants.

[0031] The embodiments of the present application also provide a product for improving drought tolerance of plants, comprising N-acetyl-L-cysteine.

[0032] The embodiments of the present application also provide a method for improving drought tolerance of plants, wherein a plant is sprayed with a N-acetyl-L-cysteine solution or the product.

[0033] In some specific embodiments, the concentration of the N-acetyl-L-cysteine solution is 150 μM.

[0034] In some specific embodiments, the product is diluted to a concentration of 150 μM of N-acetyl-L-cysteine.

[0035] In some specific embodiments, the method of the spray treatment is that the spray treatment is performed on the leaves of the plants at the seedling stage.

[0036] The fresh weight, dry weight, root length, chlorophyll content of the oilseed rape are increased, the leaf water loss rate is reduced, and the drought stress tolerance of the oilseed rape is improved by using N-acetyl-L-cysteine solution with a concentration of 150 μM to perform foliar spray treatment on the oilseed rape.

[0037] Example 1

[0038] N-acetyl-L-cysteine solution foliar spray treatment

[0039] The oilseed rape seeds are oscillation disinfected with '84' disinfectant solution (chlorine content ~ 5%) for 10 minutes, rinsed with double distilled water for 3 times, and then placed in a culture dish with double-layer filter paper for seed germination. After 3 days of germination, the oilseed rape with uniform growth vigor is selected and transferred to a hydroponic box for hydroponic growth. The hydroponic solution is Hogland nutrient solution, which is replaced every 3 days. After continuous replacement for 3 times, N-acetyl-L-cysteine solution with concentrations of 100, 150, 200, and 250 μM is used for foliar spray treatment of the oilseed rape, and the oilseed rape sprayed with pure water without N-acetyl-L-cysteine is used as a control group. The Hogland nutrient solution is replaced every 3 days, and different concentrations of N-acetyl-L-cysteine solution are used for foliar spray treatment.

[0040] Example 2

[0041] PEG6000 drought stress treatment

[0042] The hydroponic solution of the above oilseed rape is replaced with Hogland nutrient solution containing 15% PEG6000 for simulated drought stress treatment, and the Hogland nutrient solution containing 15% PEG6000 is replaced every 3 days. At the same time, the Hogland nutrient solution without PEG6000 is used as a control group (Mock), and the replacement is continuously performed for 3 times. The plant live phenotype is as shown in Figure 1 The control group (Mock) is the Hogland nutrient solution without the addition of PEG6000, and the experimental group 15% PEG6000 is the simulated drought stress treatment.

[0043] The ability of N-acetyl-L-cysteine ​​to influence the drought stress tolerance of Brassica napus was comprehensively assessed by measuring the fresh weight, dry weight, root length, chlorophyll content, and leaf water loss rate of each experimental and control group. The fresh weight, dry weight, root length, chlorophyll content, and leaf water loss rate of a single plant are five important plant physiological indicators that reflect the plant's stress resistance level. Under the same drought treatment conditions, plants that maintained higher fresh weight, dry weight, root length, and chlorophyll content showed better drought resistance, while lower leaf water loss rates indicated stronger water storage capacity and better drought tolerance.

[0044] Example 3

[0045] Optimization of N-acetyl-L-cysteine ​​solution concentration

[0046] Data from the experimental and control groups were analyzed. Individual plants were taken, and the chlorophyll content of the leaves was measured using a chlorophyll meter. After removing excess water from the roots, the fresh weight of each plant was measured, and the root length was also measured. The plants were then placed in a 65℃ oven for 24 hours, and their dry weight was measured. Fresh leaves were cut and weighed (M0), then placed in a temperature- and humidity-controlled treatment room and weighed at 3-hour intervals (M3). The leaf water loss rate was then calculated as: Leaf water loss rate (%) = (M0 - M3) / M0. The statistical results are shown in [the table below]. Figures 2-6 .

[0047] Depend on Figure 2 It was found that the fresh weight of individual plants after spraying with N-acetyl-L-cysteine ​​solutions of 100, 150, 200, and 250 μM was significantly higher than that of the control group sprayed with pure water under normal conditions, with the highest fresh weight per plant at 150 μM. The fresh weight of individual plants after spraying with N-acetyl-L-cysteine ​​solutions of 100, 150, 200, and 250 μM was also significantly higher than that of the control group sprayed with pure water under drought stress conditions, with the highest fresh weight per plant at 150 μM.

[0048] Depend on Figure 3 It was found that the dry weight of individual plants after spraying with 150 and 200 μM N-acetyl-L-cysteine ​​solutions was significantly higher than that of the control group sprayed with pure water under normal conditions, with the highest dry weight of individual plants observed at 150 μM. The dry weight of individual plants after spraying with 100, 150, 200, and 250 μM N-acetyl-L-cysteine ​​solutions was also significantly higher than that of the control group sprayed with pure water under drought stress conditions, with the highest dry weight of individual plants observed at 150 μM.

[0049] Depend on Figure 4It was found that the root length after spraying with a 150 μM N-acetyl-L-cysteine ​​solution was significantly increased compared to the control group sprayed with pure water under normal conditions, while the other dimensions showed no significant difference compared to the control. The root lengths after spraying with 100, 150, 200, and 250 μM N-acetyl-L-cysteine ​​solutions were all significantly increased compared to the control group sprayed with pure water under drought stress conditions, with the largest root length observed at 150 μM.

[0050] Depend on Figure 5 It was found that the chlorophyll content after spraying with 100, 150, 200, and 250 μM N-acetyl-L-cysteine ​​solutions did not show significant differences compared to the control group sprayed with pure water under normal conditions. The chlorophyll content after spraying with 100, 150, 200, and 250 μM N-acetyl-L-cysteine ​​solutions also did not show significant differences compared to the control group sprayed with pure water under drought stress conditions, but the average chlorophyll content was highest at 150 μM.

[0051] Depend on Figure 6 It was found that the water loss rate of leaves after spraying with 150, 200, and 250 μM N-acetyl-L-cysteine ​​solutions was significantly lower than that of the control group sprayed with pure water under normal conditions, while the difference was not significant under the 100 μM treatment. The water loss rate of leaves after spraying with 100 and 150 μM N-acetyl-L-cysteine ​​solutions was significantly lower than that of the control group sprayed with pure water under drought stress conditions, but the difference was not significant under the 200 and 250 μM conditions.

[0052] comprehensive Figure 1 Phenotype and Figures 2-6 The statistical results of physiological index detection showed that after foliar spraying of Brassica napus with a 150 μM N-acetyl-L-cysteine ​​solution, the fresh weight, dry weight, root length, and chlorophyll content were significantly increased compared with the control, while the leaf water loss rate was significantly reduced. All physiological indicators of stress resistance were superior to the control group and other concentration treatment groups. Therefore, it is concluded that foliar spraying of Brassica napus with a 150 μM N-acetyl-L-cysteine ​​solution can effectively improve the drought stress tolerance of plants, which is of great significance for production practice.

[0053] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the implementation of the present invention. For those skilled in the art, other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively describe all embodiments here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the scope of protection of the claims of the present invention.

Claims

1. A method for improving the drought resistance of plants, characterized in that, Plants were treated by spraying with N-acetyl-L-cysteine ​​solution; The concentration of the N-acetyl-L-cysteine ​​solution was 150 μM; The spraying treatment method is as follows: spray the leaves of the plant during the seedling stage.

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