Multi-channel small animal inhalation anesthesia induction box

Abstract

The invention discloses a multi-channel small animal inhalation anesthesia induction box, and relates to the field of anesthetics animal research. In the prior art, a test cavity of a transparent induction box is divided into a plurality of experiment cavities by partition plates, and adjacent experiment cavities are communicated, so that only a single-concentration anesthesia experiment can be carried out. The invention provides a multi-channel small animal inhalation anesthesia induction box. An anesthesia induction area, an adjuster and an experiment area are arranged in an induction box main body. In the anesthesia induction area, the size of an anesthesia box is controlled through a curved induction channel, an adjuster and an anesthesia injection cavity, use of anesthetic gas is reduced, the concentration of the anesthetic gas is monitored and adjusted in real time through an anesthetic gas adjusting and monitoring assembly and a waste gas treatment mechanism, meanwhile, waste gas obtained after an experiment is subjected to harmless treatment, and the environment is protected. The safety performance of the experimental device is improved, and the experimental result is more intuitive and accurate through real-time experimental data comparison of a plurality of same experimental devices in the experimental area.

Description

Technical Field

[0001] The invention relates to the field of anesthesiology animal research, in particular to a multi-channel small animal inhalation anesthesia induction box. Background Art

[0002] The basic task of anesthesia is to eliminate the pain and discomfort caused during the experiment, ensure the safety of experimental animals, make the animals obey the operation in the experiment, and ensure the smooth progress of the experiment. Among them, inhalation anesthesia, also known as gas anesthesia, is a commonly used method of anesthesia. Anesthetics are inhaled through the respiratory tract in the form of vapor or gas to produce anesthesia, which is called inhalation anesthesia, and ether is commonly used as an anesthetic. Inhalation anesthesia is usually suitable for animal experiments with a short anesthesia time, or used as basic anesthesia, or as an auxiliary anesthesia for injection anesthesia. It has a fast onset, stable anesthetic effect, and the animal wakes up quickly. The depth and duration of anesthesia are easy to control. It is considered to be the most reliable anesthesia method for rodents. The disadvantage is that it is more suitable for medium and small animals. For large animals such as dogs, the inhalation anesthesia operation is complicated and is usually not used.

[0003] In the field of anesthesia animal research, it is often necessary to measure the sensitivity of small animals to inhaled anesthetics in routine anesthesia experiments. This measurement not only helps to study the sensitivity of small animals with certain gene mutations to existing inhaled anesthetics to determine the targets of inhaled anesthetics, but can also be used to evaluate the anesthetic effects of new anesthetics on small animals. Therefore, it is particularly important to develop an efficient, accurate and multi-channel induction box for small animal inhaled anesthetic dose measurement.

[0004] The ‌Multi-channel Animal Anesthesia Induction Chamber‌ is a device specially used for pre-anesthesia treatment of experimental animals. It uses high-concentration, high-flow anesthetic gas to put the animal into an anesthesia state in a very short time (one or two minutes) for operations such as restraint or preoperative treatment. This type of equipment is usually made of transparent materials, which makes it easy for experimenters to observe the anesthetic state of the animal. Due to the use of high-concentration, high-flow anesthetic gas, it must be carried out in a sealed space, such as an anesthesia induction box or anesthesia induction chamber, to ensure rapid results and facilitate exhaust gas collection and treatment. ‌ After searching, a Chinese patent with patent number CN105527416A discloses a multi-channel small animal inhalation anesthetic dosage measurement induction box, including a square or cylindrical transparent induction box and a sealed box cover compatible with the transparent induction box. The transparent induction box and the sealed box cover enclose a test chamber. The transparent induction box is provided with an air inlet for introducing gaseous anesthetics into the test chamber and an air outlet for exhausting the gaseous anesthetics. The air outlet is provided with a concentration monitor. A number of partitions are provided in the test chamber. The partitions divide the test chamber into a number of unit test chambers and the adjacent two unit test chambers are connected. An opening connecting to the outside is provided on the wall of each unit test chamber.

[0005] The existing technology has the following problems: 1. Waste of gaseous anesthetics. Since the closed induction box has a large volume and needs to frequently introduce high-flow gaseous anesthetics to maintain the concentration balance in the box, a large amount of gaseous anesthetics is wasted; 2. The operation is complicated. The tail-flick reaction experiment and the righting reflex experiment require the replacement of the induction box, resulting in a long experimental cycle and low efficiency; 3. The experimental efficiency is low. The gaseous anesthetics in the multi-channel induction box are of the same concentration, there is no comparison effect, and multiple experiments need to be carried out; 4. The sealing effect is poor and the gaseous anesthetic overflows. Summary of the invention

[0006] In view of the above-mentioned problems, the present invention aims to provide a multi-channel small animal inhalation anesthesia induction box, which can improve experimental efficiency, reduce anesthetic waste, and add anesthesia boxes and induction experimental devices with different concentrations to form a contrast effect.

[0007] The main ideas of the technical solution adopted by the present invention are: multi-channel design, which can carry out experiments on multiple small animals at the same time, greatly improving the experimental efficiency; using the inflation characteristics of the airbag to adjust the size of the anesthesia box, saving gaseous anesthetics and not wasting resources; using a concentration monitoring mechanism to monitor the concentration of anesthetic gas in real time, and controlling the piston injection mechanism according to experimental requirements to adjust the concentration of anesthetics in the anesthesia box; setting up multiple anesthesia boxes to carry out anesthesia experiments of various concentrations, turning experiment mechanisms, maze experiment mechanisms, balance experiment mechanisms and tail-flicking experiment mechanisms, reducing experimental operation steps, improving experimental efficiency, and performing comparisons.

[0008] In order to achieve the above object, the technical solution adopted by the present invention is as follows: The multi-channel small animal inhalation anesthesia induction box comprises an induction box base, an induction box body and an induction box cover, wherein the induction box base is rotatably connected to the induction box body, the induction box cover is arranged above the induction box body, and the induction box cover and the induction box body enclose a test chamber, characterized in that it also comprises an anesthesia induction core cylinder concentric with the induction box body, and a plurality of partition plates arranged in the induction box body divide the anesthesia induction core cylinder and the induction box body into a plurality of experimental areas; wherein, The anesthesia induction core tube is provided with a plurality of identical anesthesia induction areas for anesthetizing small animals and a regulator capable of adjusting the size of an anesthesia channel for small animals; The experimental area includes a righting experimental mechanism, a maze experimental mechanism, a balance experimental mechanism and a tail-flicking experimental mechanism which are arranged in a connected manner.

[0009] Through the above technical solution, further: the anesthesia induction area includes a curved induction channel and an anesthesia injection cavity arranged adjacent to the curved induction channel, and an anesthesia device is arranged in the anesthesia injection cavity.

[0010] Through the above technical solution, further: the anesthesia apparatus includes an anesthesia device for anesthetizing small animals and a concentration monitoring mechanism for monitoring the concentration of anesthetic gas in the anesthesia device.

[0011] Through the above technical solution, further: the regulator is arranged on the side wall of the curved induction channel, including a channel regulating mechanism and a channel sealing switch mechanism, and the channel regulating mechanism and the channel sealing switch mechanism are connected through a ventilation duct.

[0012] Through the above technical scheme, further: the straightening experimental mechanism includes an upper straightening bracket, a lower straightening bracket, a middle sliding bracket and a folding enclosure, the middle sliding bracket is slidably connected to the lower straightening bracket, the middle sliding bracket supports the upper straightening bracket, a first magnet is installed on one side of the middle sliding bracket, and a second magnet is arranged at a corresponding position of the folding enclosure, a magnetic attraction force is formed between the first magnet and the second magnet, the middle sliding bracket moves downward to drive the first magnet to move downward, so that the first magnet and the second magnet are misaligned, the linked helical torsion spring and the torsion shaft rotate synchronously, and drive the straightening experimental mechanism to rotate together, so as to realize the straightening of the device.

[0013] Through the above technical scheme, further: the maze experimental mechanism includes a cross-shaped maze, door one and door two, the cross-shaped maze is a cross-shaped hollow structure, one end of which is provided with door one, the open end of door one is inserted into a folding enclosure, and the other end of which is provided with door two, which is set on the maze experimental mechanism.

[0014] Through the above technical scheme, further: the balance experiment mechanism includes an arc-shaped balance lever, a balance spring and a balance connecting rod, the middle part of the arc-shaped balance lever is fixedly connected to the balance connecting rod, one end is overlapped under the second cross-shaped maze door, and the other end is fixedly connected to the balance spring.

[0015] Through the above technical scheme, further: the tail-flicking experiment mechanism includes a restraint part and a tail-flicking experiment part, the restraint part and the tail-flicking experiment part are arranged adjacent to each other, the valve of the restraint part is mounted above the valve mounting hole by an L-shaped slide bar, after the L-shaped slide bar moves, the valve falls to restrain and fix the head of the small animal, and the tail-flicking experiment part stimulates the tail of the small animal.

[0016] Through the above technical scheme, further: a multi-channel small animal inhalation anesthesia induction experimental method is implemented using a multi-channel small animal inhalation anesthesia induction box, comprising the following steps: Place the small animal into the induction box body, rotate the induction box body so that the induction box base and the induction box body are staggered and sealed, and close the induction box lid; After the small animal enters the curved induction channel through the straight induction channel, the channel regulating mechanism is used to regulate the space of the curved induction channel, and the synchronous channel sealing switch mechanism is used to seal the curved induction channel to form an anesthesia device; The concentration of anesthetic gas in the anesthesia device is monitored in real time by a concentration monitoring mechanism, and the concentration of anesthetic gas in the anesthesia device is adjusted by regulating the anesthesia device according to experimental needs; After the small animals are anesthetized with different concentrations of anesthetic doses, the uprighting experimental data can be recorded through the uprighting experimental mechanism to test the degree of anesthesia at different concentrations of anesthetic doses and compare the experimental data of the four channels at the same time; After the small animal righting experiment, the time and number of errors of the small animal were measured through the maze experiment mechanism to determine the effects of different concentrations of anesthetics on the cognitive function of the small animal. After the small animal maze experiment is completed, the time and number of errors of the small animal are measured by the balance experiment mechanism to determine the effect of different concentrations of anesthetics on the balance of the small animal after anesthesia; After the small animal balance experiment is completed, the small animal's tail is stimulated with electric current through the tail-flicking experiment mechanism, and the reaction time of the small animal to quickly flick its tail after feeling pain is recorded to evaluate the animal's sensitivity to pain and pain threshold.

[0017] The beneficial effects of the present invention are: 1. Improve experimental accuracy The anesthesia devices are set in multiple induction channels to measure the responses of multiple small animals under the same or different anesthetic drug concentrations at the same time, thereby reducing the variability and error between experiments and improving the accuracy and reliability of experimental data; 2. Reduce animal suffering: In the anesthetic gas regulation and monitoring component, the concentration of anesthetics in the anesthesia box can be monitored in real time through the piston injection mechanism and the concentration monitoring mechanism, and the concentration of anesthetics in the anesthesia box can be regulated according to the experimental requirements. The dosage and concentration of anesthetics can be accurately controlled, which can minimize the pain and discomfort of small animals during the experiment, in line with the principle of animal welfare protection; 3. Experimental efficiency Improve experimental efficiency: The induction box is equipped with four identical induction channels, anesthesia devices and induction experimental devices, which can observe and record multiple samples at the same time, significantly shorten the experimental cycle, improve the efficiency and accuracy of experimental operations, and greatly save time and labor costs; Convenient for data comparison and analysis: All samples in the multi-channel induction chamber are tested at the same time point and in the same device, so the data obtained are more comparable, which is convenient for subsequent data comparison and analysis; 4. Reduce anesthetic waste Taking advantage of the free inflation feature of the airbag, the size of the airbag can be changed by inflation when conducting experiments on small animals of different sizes, thereby controlling the size of the channel and thus controlling the amount of anesthetic gas required for the anesthesia experiment, thereby achieving the purpose of the most reasonable use of anesthetic gas; 5. Improve sealing The base of the induction box is rotatably connected to the main body of the induction box via a rotating shaft, so the first inlet and the second inlet can be aligned and staggered by rotating the main body of the induction box. There is no need to open the lid to place small animals, thereby improving the sealing of the device. At the same time, the anesthesia box is arranged in the induction channel and is sealed by a channel sealing switch mechanism, thereby further enhancing the sealing of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments are briefly introduced below.

[0019] Figure 1 It is a schematic diagram of the structure of the present invention; Figure 2 This is a schematic diagram of the structure of the induction box cover of the present invention; Figure 3 This is a schematic diagram of the structure of the induction box base of the present invention; Figure 4 This is a schematic diagram of the main structure of the induction box of the present invention; Figure 5 It is a top view of the induction box body of the present invention; Figure 6 This is a schematic diagram of the structure of the induction channel and the anesthesia device of the present invention; Figure 7 This is a schematic diagram of the structure of the anesthesia box of the present invention; Figure 8 It is a schematic diagram of the structure of the waste gas treatment mechanism of the present invention; Fig. 9 This is a schematic diagram of the structure of the piston injection mechanism of the present invention; Fig.10 This is a schematic diagram of the structure of the experiment mechanism of the present invention; Fig.11 This is a schematic diagram of the structure of the upper uprighting bracket of the uprighting experimental mechanism of the present invention; Fig.12 This is a schematic diagram of the structure of the lower righting bracket of the righting experimental mechanism of the present invention; Fig.13 This is a schematic diagram of the structure of the sliding bracket in the middle of the uprighting experimental mechanism of the present invention; Fig.14 This is a schematic diagram of the structure of the maze experiment mechanism of the present invention; Fig.15 It is a structural schematic diagram of the balance experiment mechanism of the present invention; Fig.16This is a schematic diagram of the structure of the tail-flick experiment mechanism of the present invention; Fig.17 This is a schematic diagram of the structure of the straight channel and the circular movable part of the present invention; Fig.18 This is a schematic diagram of the structure of the stimulation part of the present invention; Fig.19 Schematic diagram of the constraint structure of the present invention. In the figure: 1. Induction box base; 101. Rotating shaft; 102. First inlet; 103.; 2. Induction box body; 201. Second inlet; 203.; 3. Induction box cover; 301. Baffle jack; 302. Anesthesia jack; 303. Exhaust hole; 4. Induction channel; 401. Straight induction channel; 402. Curved induction channel; 403. Exit; 404. Baffle 5. Channel adjustment mechanism; 501. Airbag; 502. Ventilation duct; 503. Inflation duct; 6. Channel sealing switch mechanism; 601. First sealing piston; 602. Second sealing piston; 603. Telescopic joint; 7. Anesthetic storage cylinder; 701. Anesthetic agent chamber; 702. Anesthetic filling hole; 703. Spray nozzle; 704. Piston mounting hole; 705. Anesthetic filling tube; 706. Anesthetic sealing cap; 8. Piston injection mechanism; 801. Pressure plate; 802. Connecting rod; 803. Piston; 804. Anesthetic injection cylinder; 805. Air outlet; 9.Concentration adjustment mechanism components; 10. Concentration monitoring mechanism; 1001. Monitoring components; 1002. Display screen; 11. Waste gas treatment mechanism; 1101. Air pump; 1102. Air extraction pipeline; 1103. Waste gas treatment device; 1104. Exhaust pipe; 12. Turning experiment mechanism; 1201. Upper turning bracket; 1202. Lower turning bracket; 1203. Middle sliding bracket; 1204. Helical torsion spring; 1205. First spring; 1206. First magnet; 1207. Folding enclosure; 1208. Second magnet; 1209. First support rod; 1210. First stopper; 1211. First support surface; 1212. Square hole 1; 1213. First panel; 1214. Second panel; 1215. Second support rod; 1216. Second stopper; 1217. Support frame; 1218. First slide; 1219. Torsion shaft; 1220. Third support rod; 1221. Sliding support plate; 1222. Second support surface; 13. Maze experiment mechanism; 1301. Cross maze; 1302. Gate 1; 1303. Gate 2; 14. Balance experiment mechanism; 1401. Arc balance lever; 1402. Balance spring; 1403. Balance connecting rod; 15. Tail-drift experiment mechanism; 1501. Gate three; 1502. Straight-moving channel; 1503. Circular movable part; 1504. Tail-drift experiment part; 1505. Constraint part; 1506. Pedal; 1507. Second spring; 1508. Spring mounting groove; 1509. Pedal mounting groove; 1510. Third spring; 1511. L-shaped slide bar; 1512. L-shaped slide groove; 1513. Valve mounting hole; 1514. Third magnet; 1515. Switch; 1516. Valve; 1517. Rubber fixing pad; 1518. Slot; 1519. Stimulation part; 1520. Sliding pin; 1521. Sliding plate; 1522. Stimulation plate; 1523. Second slide groove; 1524. Third slide groove; 1525. First slide bar; 1526. Limit plate; 1527. Pin hole. DETAILED DESCRIPTION

[0020] In order to make the purpose, technical solution and advantages of the embodiments of the present application clearer, the technical solution in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. The components of the embodiments of the present application described and shown in the drawings here can be arranged and designed in various different configurations.

[0021] Therefore, the following detailed description of the embodiments of the present application provided in the accompanying drawings is not intended to limit the scope of the present application for which protection is sought, but merely represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in the field without creative work are within the scope of protection of the present application.

[0022] The inventors have found in practice that the existing devices have the following problems: the closed induction box has a large volume, resulting in a large amount of waste of gaseous anesthetics; the tail-flick reaction experiment and the righting reflex experiment require the replacement of the induction box, resulting in a long experimental cycle and low efficiency; the gaseous anesthetics in the multi-channel induction box have the same concentration, there is no comparison effect, and multiple experiments need to be carried out; the sealing effect is poor, and the gaseous anesthetics overflow.

[0023] Based on the above findings, the present application proposes a multi-channel small animal inhalation anesthesia induction box, which can improve experimental efficiency, reduce anesthetic waste, and at the same time form a contrast effect. Example

[0024] See also Figures 1 to 19The present application discloses a multi-channel small animal inhalation anesthesia induction box, including an induction box base 1, an induction box body 2 and an induction box cover 3. The induction box base 1 is rotatably connected to the induction box body 2, the induction box cover 3 is arranged above the induction box body 2, and the induction box cover 3 and the induction box body 2 enclose a test chamber.

[0025] The induction box cover 3 is provided with a baffle jack 301, an anesthesia jack 302 and an exhaust hole 303. The baffle 404 can be lifted at any time through the baffle jack 301 to open the outlet 403; the anesthesia jack 302 is inserted into the connecting rod 802 of the piston injection mechanism 8, and the anesthesia box can be directly filled with anesthetics on the pressure plate 801 outside the induction box; the exhaust hole 303 is inserted into the exhaust pipe 1104 of the waste gas treatment mechanism 11, and the purified gas is discharged into the air through the exhaust pipe 1104 arranged above the waste gas treatment device 1103. The induction box cover 3 is made of transparent material, which is convenient for experimenters to observe directly from the outside.

[0026] The side wall of the induction box base 1 is evenly provided with four first entrances 102, and a rotating shaft 101 is arranged in the center; the side wall of the induction box body 2 is evenly provided with four second entrances 201, and the induction box base 1 is rotatably connected to the induction box body 2 via the rotating shaft 101. Before placing the small animal, the induction box body 2 is rotated to make the first entrance 102 and the second entrance 201 overlap. After placing the small animal, the induction box body 2 is rotated to make the first entrance 102 and the second entrance 201 staggered and closed.

[0027] The induction box body 2 is concentrically provided with an anesthesia induction core barrel, and a plurality of partitions arranged inside the induction box body 2 divide the anesthesia induction core barrel and the induction box body 2 into a plurality of experimental areas; the anesthesia induction core barrel is provided with a plurality of identical anesthesia induction areas for anesthetizing small animals and a regulator capable of adjusting the size of the anesthesia channel for small animals, and an exhaust gas treatment mechanism 11 is arranged in the center of the anesthesia induction core barrel.

[0028] The anesthesia induction area includes a curved induction channel 402, wherein the induction channel 4 includes a straight induction channel 401 and a curved induction channel 402, one end of the straight induction channel 401 is connected to the second entrance 201, and the other end is connected to the curved induction channel 402, one end of the curved induction channel 402 is connected to the exit 403, and adjacent curved induction channels 402 are tangent to each other. The small animal enters the straight induction channel 401 and the curved induction channel 402 in turn from the second entrance 201, lifts the baffle 404 to open the exit 403, and then enters the experimental area from the exit 403.

[0029] The anesthesia induction area also includes an anesthesia injection cavity adjacent to the curved induction channel 402. The anesthesia device is disposed in the anesthesia injection cavity. The anesthesia device includes a concentration monitoring mechanism 10 for anesthetizing the small animal through the anesthesia device and regulating and monitoring the concentration of anesthetic gas in the anesthesia device.

[0030] The anesthesia device includes an anesthetic storage cylinder 7, a piston injection mechanism 8 and an anesthesia box.

[0031] The anesthetic storage cylinder 7 is a curved cavity, a piston mounting hole 704 is provided in the middle of the curved cavity, an anesthetic filling hole 702 is provided at the edge, an anesthetic agent cabin 701 is sleeved in the curved cavity, a piston mounting hole 704 and an anesthetic agent filling hole 702 are also provided at the corresponding position of the anesthetic agent cabin 701, an anesthetic agent filling tube 705 is inserted into the anesthetic agent filling hole 702 at the lower end, and an anesthetic agent sealing cap 706 is connected at the upper end, and anesthetic agents of different concentrations are pressurized and injected into the anesthetic agent cabin 701 through the anesthetic agent filling tube 705, and the pressurization range is 1.5~2.0kg / cm²; a plurality of nozzles 703 are provided on the side wall of the anesthetic agent cabin 701, and the pressure range of the nozzles 703 is usually about 2~4kg / cm², and the nozzles 703 are staggered with the airbag 501 to prevent the airbag 501 from blocking the atomizing nozzle 703 after expansion, so that the nozzles 703 cannot allow anesthetics to be filled into the anesthesia box.

[0032] The piston injection mechanism 8 includes a pressure plate 801, a connecting rod 802, a piston 803, an anesthetic injection cylinder 804 and an air outlet 805. One end of the connecting rod 802 is connected to the pressure plate 801, and the other end is connected to the piston 803. The piston 803 is slidably installed in the anesthetic injection cylinder 804. The air outlet 805 is opened just below the anesthetic injection cylinder 804. The anesthetic injection cylinder 804 has a scale, and the anesthetic dosage can be measured by reading the value of the piston 803 being pressed down. The piston injection mechanism 8 is installed on the anesthetic storage cylinder 7 through the piston installation hole 704.

[0033] The anesthesia box is an anesthesia sealed cavity composed of a curved induction channel 402 and a regulator. The regulator is arranged on the side wall of the curved induction channel 402, and includes a channel regulating mechanism 5 and a channel sealing switch mechanism 6, which are connected through a ventilation pipe 502.

[0034] The channel regulating mechanism 5 is installed on the side of the curved induction channel 402 close to the anesthetic storage cylinder 7. The channel regulating mechanism 5 includes an airbag 501, a ventilation pipe 502 and an inflation pipe 503. The inflation pipe 503 is arranged above the airbag 501. By utilizing the characteristic that the airbag 501 can be freely inflated, the size of the airbag 501 can be changed by inflation when conducting experiments on small animals of different sizes, thereby controlling the size of the channel and further controlling the amount of anesthetic gas required for the anesthesia experiment, thereby achieving the purpose of the most reasonable use of anesthetic gas.

[0035] The channel regulating mechanism 5 and the channel sealing switch mechanism 6 are connected through the ventilation pipe 502. The two channel sealing switch mechanisms 6 are fixedly installed on the side wall of the anesthetic storage cylinder 7. The second sealing piston 602 is installed in the first sealing piston 601. The telescopic joint 603 is installed between the first sealing piston 601 and the second sealing piston 602. The ventilation pipe 502 inflates the first sealing piston 601 and the second sealing piston 602. The second sealing piston 602 starts to move relative to the first sealing piston 601, thereby driving the telescopic joint 603 to perform telescopic movement until the second sealing piston 602 fits and seals the curved induction channel 402 to achieve sealing of the channel.

[0036] The concentration monitoring mechanism 10 is arranged above the anesthesia box, and includes a monitoring component 1001 and a display screen 1002. The monitoring component 1001 and the display screen 1002 are electrically connected. The monitoring component 1001 is a gas anesthesia concentration sensor. The monitoring component 1001 is inserted into the anesthesia box to monitor the concentration of anesthetic agents in the anesthesia box and display the concentration value of anesthetic agents in the anesthesia box in real time through the display screen 1002.

[0037] The concentration regulating mechanism assembly 9 includes a piston injection mechanism 8 and a concentration monitoring mechanism 10. The concentration of the anesthetic in the anesthesia box is monitored in real time by the concentration monitoring mechanism 10. Then, according to the experimental requirements, the pressure plate 801 is pressed to adjust the concentration of the anesthetic in the anesthesia box.

[0038] The waste gas treatment mechanism 11 includes an exhaust pump 1101, an exhaust pipe 1102, a waste gas processor 1103 and an exhaust pipe 1104. The exhaust pipe 1102 connects the anesthesia box and the exhaust pump 1101. The exhaust pump 1101 extracts the waste gas after the experiment in the anesthesia box, and then the waste gas processor 1103 at the upper end of the channel treats the anesthetic gas harmlessly. When extracting the anesthetic gas, in order to avoid the internal vacuum causing harm to the experimental animals, it is necessary to simultaneously deflate the airbag so that the air at both ends can enter the anesthesia box. The purified gas is discharged into the air through the exhaust pipe 1104 arranged above the waste gas processor 1103. When the extraction is completed, the baffle 404 is lifted to proceed to the next experiment.

[0039] A usage process of this embodiment is: Four small animals with the same or similar physiological conditions were selected, and after one week of adaptation to the experimental environment, the experiment was conducted. The induction box body 2 was adjusted so that the four first entrances 102 and the second entrance 201 were aligned, and the four small animals were placed in the second entrances 201 respectively. The induction box body 2 was rotated so that the first entrance 102 and the second entrance 201 were staggered and closed.

[0040] The small animal enters the straight induction channel 401 and the curved induction channel 402 in sequence through the second entrance 201. After entering the curved induction channel 402, Immediately inflate the airbag 501 through the inflation pipe 503 to expand the airbag, and clamp the small animal between the airbag 501 and the curved induction channel 402 to fix the small animal. Continue to inflate the airbag 501, and the gas is squeezed from the ventilation pipe 502 into the first sealing piston 601 and the second sealing piston 602. Then continue to inflate the airbag 501. The gas squeezes the second sealing piston 602 to start moving relative to the first sealing piston 601, thereby driving the telescopic joint 603 to perform telescopic movement until the second sealing piston 602 fits tightly with the curved induction channel 402, thereby sealing the channel and forming an anesthesia box.

[0041] Pressing the pressure plate 801 drives the connecting rod 802 and the piston 803 to move in the anesthetic injection cylinder 804, causing the pressure in the anesthetic injection cylinder 804 to rise, and the pressure is transmitted to the anesthetic agent chamber 701 through the air outlet 805. Continue to press the pressure plate 801 to increase the pressure in the anesthetic agent chamber 701, allowing the anesthetic in the anesthetic agent chamber 701 to be filled into the anesthesia box through the nozzle 703.

[0042] The concentration of the anesthetic in the anesthesia box is monitored in real time by the concentration monitoring mechanism 10 , and the concentration of the anesthetic in the anesthesia box is adjusted by pressing the pressure plate 801 according to the experimental requirements.

[0043] After the anesthesia of the small animal's head is completed, the waste gas after the experiment in the anesthesia box is extracted through the vacuum pump 1101, and the waste gas processor 1103 at the upper end of the channel treats the anesthetic gas harmlessly. When extracting the anesthetic gas, in order to avoid the internal vacuum causing harm to the experimental animals, it is necessary to deflate the airbag simultaneously so that the air at both ends can enter the anesthesia box. The purified gas is discharged into the air through the exhaust pipe 1104 arranged above the waste gas processor 1103. When the extraction is completed, the baffle 404 is lifted to proceed to the next experiment. Example

[0044] The experimental area includes a righting experimental mechanism 12, a maze experimental mechanism 13, a balance experimental mechanism 14 and a tail-flicking experimental mechanism 15 which are connected to each other in sequence.

[0045] The uprighting experimental mechanism 12 is composed of an upper uprighting bracket 1201 , a lower uprighting bracket 1202 , a middle sliding bracket 1203 and a folding enclosure 1207 .

[0046] The upper turning bracket 1201 is composed of the following structures: the bottoms of the four first support rods 1209 are respectively fixedly connected to the first stoppers 1210; the upper parts of the four first support rods 1209 are horizontally fixedly connected to the first support surface 1211; a square hole 1212 is provided on the first support surface 1211; the square hole 1212 has two opposite sides on one side hingedly connected to the first panel 1213 and the other side fixedly connected to the second panel 1214; the first panel 1213 and the second panel 1214 form a one-way door.

[0047] The lower uprighting bracket 1202 is composed of the following structures: the first springs 1205 are respectively sleeved on the four second support rods 1215, the bottoms of the four second support rods 1215 are respectively fixedly connected to the second stop blocks 1216, the upper part is horizontally fixedly connected to the support frame 1217, and the first sliding grooves 1218 are respectively provided on the opposite sides of the support frame 1217; a torsion shaft 1219 is fixedly installed on one of the end faces of the support frame 1217, and a spiral torsion spring 1204 is sleeved on the torsion shaft 1219.

[0048] The structure of the middle sliding bracket 1203 is as follows: four third support rods 1220 penetrate the second support surface 1222 and are fixedly connected to the second support surface 1222. The upper parts of the four third support rods 1220 horizontally support the first support surface 1211. The bottoms of the four third support rods 1220 are vertically connected to the sliding support plate 1221 in an "L" shape. The sliding support plate 1221 is slidably sleeved on the second support rod 1215, and the sliding support plate 1221 is arranged on the upper end of the first spring 1205. The second support surface 1222 is set in the support frame 1217. The first magnet 1206 is installed on one side of the middle sliding bracket 1203, and the second magnet 1208 is arranged at the corresponding position of the folding enclosure 1207.

[0049] The righting experiment mechanism 12 is linked: the small animal's own gravity triggers the sliding support plate 1221 to compress the first spring 1205 to move downward and drive the middle sliding bracket 1203 to move downward. Because the first support rod 1209 is inserted in the first slide groove 1218, and the top of the third support rod 1220 of the middle sliding bracket 1203 is horizontally supported on the four corners of the first support surface 1211 of the upper righting bracket 1201, the upper righting bracket 1201 moves downward, so that the distance between the upper righting bracket 1201 and the lower righting bracket 1202 is reduced; because the first The magnet 1206 is installed on the middle sliding bracket 1203. The middle sliding bracket 1203 drives the first magnet 1206 to move downward, causing the first magnet 1206 to be misaligned with the second magnet 1208 installed on the folding enclosure 1207, causing the spiral torsion spring 1204 to rotate. The spiral torsion spring 1204 drives the torsion shaft 1219 to rotate synchronously. The torsion shaft 1219 drives the three connected upper correction brackets 1201, lower correction brackets 1202 and middle sliding brackets 1203 to rotate together to realize the correction of the device.

[0050] A usage process of this embodiment is: after anesthesia with different concentrations of anesthetic doses, the small animal comes out from the exit 403, enters between the upper righting bracket 1201 and the lower righting bracket 1202, and stands on the middle sliding bracket 1203. Due to the gravity of the small animal itself, the middle sliding bracket 1203 is triggered to move down. While the middle sliding bracket 1203 moves down, it drives the first magnet 1206 to move down, so that the first magnet 1206 and the second magnet 1208 are misaligned, the magnetic attraction is disconnected, and the linked spiral torsion spring 1204 is turned 180 degrees. After turning 180 degrees, the small animal and the first panel 1213 are pressed by their own gravity to open the one-way door at the lower end. During the downward movement, the second panel 1214 plays a limiting role to ensure that the small animal lands in a supine position after turning 180 degrees. At this time, the righting experiment data can be recorded, and the experimental data of the four channels can be compared at the same time. After the experiment is completed, reset it and wait for the next experiment. Example

[0051] The maze experiment mechanism 13 includes a cross-shaped maze 1301, a first door 1302 and a second door 1303. The cross-shaped maze 1301 is a cross-shaped hollow structure, with a first door 1302 at one end, and a folding enclosure 1207 is inserted into the opening end of the first door 1302, so that the small animals can enter the maze experiment after the righting experiment is completed. A second door 1303 is opened at one end, and the second door 1303 is set on the arc-shaped balance lever 1401 and is perpendicular to the passage inserted into the folding enclosure 1207, so that the small animals can enter the balance experiment 14 after the maze experiment is completed. The other two ends are closed without opening the passage, and there are sticky glue or nails on the road section to force the small animals to turn to the correct road.

[0052] A use process of this embodiment is: the small animal enters the cross-shaped maze 1301 from the first gate 1302. When the small animal enters the wrong channel at the other two ends, the sticky glue or nails provided in the channel section force the small animal to turn to the correct channel and then exit from the second gate 1303 of the correct channel. By measuring the time and number of errors of the small animal passing through, the effect of different concentrations of anesthetic agents on the cognitive function of the small animal after anesthesia is determined. Example

[0053] The balance experiment mechanism 14 includes an arc-shaped balance lever 1401, a balance spring 1402 and a balance connecting rod 1403. The middle part of the arc-shaped balance lever 1401 is fixedly connected to the balance connecting rod 1403, one end of which is overlapped under the second gate 1303 of the cross-shaped maze 1301, and the other end is fixedly connected to the balance spring 1402.

[0054] A usage process of this embodiment is: the small animal enters the balance experiment from the second passage 1303, and stands in the middle of the arc-shaped balance lever 1401. When the small animal enters the wrong end, this end drops due to its own gravity, and the balance spring 1402 stretches to cushion the direct fall of the wrong end to prevent the small animal from being injured. At the same time, sticky glue or nails are provided on the wrong end to force the small animal to walk into the correct channel in the opposite direction. By measuring the time the small animal passes and the number of errors, the influence of different concentrations of anesthetics on the balance of the small animal after anesthesia is determined. Example

[0055] The tail-flick experiment mechanism 15 is composed of a straight passage 1502, a circular movable part 1503, a tail-flick experiment part 1504 and a restraining part 1505. One end of the straight passage 1502 is hinged to the door 3 1501, and the other end is fixedly connected to the circular movable part 1503. The tail-flick experiment part 1504 is arranged at the junction of the straight passage 1502 and the circular movable part 1503. The tail-flick experiment part 1504 includes a restraining part 1505 and a stimulating part 1519. The stimulating part 1519 is adjacently arranged behind the restraining part 1505. The restraining part 1505 and the stimulating part 1519 are linked by the electrical connection between the switch 1515 and the stimulating plate 1522.

[0056] The constraining part 1505 is composed of the following structures: the two sides of the pedal 1506 are installed in the pedal installation groove 1509, the bottom of the pedal 1506 is clamped in the spring installation groove 1508, the bottoms of the two second springs 1507 are fixedly connected to the spring installation groove 1508, and the tops of the two second springs 1507 support the bottom of the pedal 1506. Two L-shaped slide grooves 1512 are respectively provided on the straight passage 1502 and the side wall of the circular movable part 1503, and penetrate the pedal installation groove 1509 and the valve installation hole 1513 above. The round rod of the L-shaped slide rod 1511 is a telescopic structure, and the third spring 1510 is compressed on the round rod. The square rod of the L-shaped slide rod 1511 penetrates the valve installation hole 1513 and is clamped in the clamping groove 1518 of the valve 1516, which has a limited support effect on the valve 1516 to prevent the valve 1516 from falling. The middle part of the valve 1516 is connected to a rubber fixing pad 1517 through a connecting rod. After the valve 1516 falls, the support rods on both sides of the valve 1516 clamp the small animal from both sides, and the rubber fixing pad 1517 fixes the small animal from the back. A third magnet 1514 is provided at the bottom of the valve mounting hole 1513 below, and a switch 1515 is provided above the third magnet 1514. After the valve 1516 falls, the support rods on both sides of the valve 1516 enter the valve mounting hole 1513 and are adsorbed and fixed by the third magnet 1514. At the same time, the switch 1515 is turned on to energize the stimulation part 1519.

[0057] The composition structure of the stimulation part 1519 is as follows: the stimulation plate 1522 is fixedly connected to the top of the sliding plate 1521 through the first sliding rod 1525, the surface of the stimulation plate 1522 is energized and slidably set on the surface of the straight channel 1502, the first sliding rod 1525 passes through the second sliding groove 1523 and is slidably connected to the second sliding groove 1523; the sliding plate 1521 is clamped in the two third sliding grooves 1524 and is slidably connected to the third sliding grooves 1524; the two sides of the sliding plate 1521 are vertically fixedly connected to the limiting plates 1526, and the two limiting plates 1526 are respectively provided with pin holes 1527 on the top, and the sliding pin 1520 is inserted into the pin hole 1527, and the sliding pin 1520 is pulled to drive the limiting plate 1526 to move, and the limiting plate 1526 drives the sliding plate 1521 to slide along the third sliding groove 1524, and the sliding plate 1521 drives the stimulation plate 1522 to slide along the second sliding groove 1523 through the first sliding rod 1525. By adjusting the position of the sliding pin 1520 and thus the stimulation part 1519, the tail-flick experimental structure 15 can meet the needs of small animals of different sizes.

[0058] A use process of this embodiment is as follows: the small animal enters the straight passage 1502 through the third passage 1501, and continues to move forward and steps on the pedal 1506. Due to the small animal's own gravity, the pedal 1506 compresses the second spring 1507 and sinks. A vacancy is formed above the spring installation slot 1508 due to the sinking of the pedal 1506, providing a channel for the compressed third spring 1510 to stretch out. Based on the elastic deformation of the compressed third spring 1510, the round rod of the L-shaped slide bar 1511 is extended and retracted to enter the vacancy formed by the spring installation slot 1508, thereby triggering the square rod of the L-shaped slide bar 1511 to move from the valve The door 1516 is disengaged from the slot 1518. Due to the gravity of the valve 1516, the valve 1516 falls. When the valve 1516 falls, the support rods on both sides of the valve 1516 clamp the small animal from both sides, and the rubber fixing pad 1517 fixes the small animal from the back. At the same time, the support rods on both sides of the valve 1516 enter the valve installation hole 1513 to trigger the switch 1515, so that the surface of the stimulation plate 1522 is electrified, and the tail of the small animal is stimulated by the current to cause pain in the tail of the small animal. The reaction time of the small animal to quickly flick its tail after feeling the pain is recorded to evaluate the animal's sensitivity to pain and pain threshold. After the experiment is completed, reset it for the next experiment.

[0059] The above shows and describes the basic principles, main features and advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments, and the above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A multi-channel small animal inhalation anesthesia induction box, comprising an induction box base (1), an induction box body (2) and an induction box cover (3), wherein the induction box base (1) is rotatably connected to the induction box body (2), the induction box cover (3) is arranged above the induction box body (2), and the induction box cover (3) and the induction box body (2) enclose a test chamber, characterized in that: It also includes an anesthesia induction core cylinder concentric with the induction box body (2), and a plurality of partition plates arranged in the induction box body (2) divide the space between the anesthesia induction core cylinder and the induction box body (2) into a plurality of experimental areas; wherein: The anesthesia induction core tube is provided with a plurality of identical anesthesia induction areas for anesthetizing small animals and a regulator capable of adjusting the size of an anesthesia channel for small animals; The experimental area comprises, in sequence, a righting experimental mechanism (12), a maze experimental mechanism (13), a balance experimental mechanism (14), and a tail-flicking experimental mechanism (15) which are arranged in a connected manner.

2. The multi-channel small animal inhalation anesthesia induction box according to claim 1, characterized in that: The anesthesia induction area comprises a curved induction channel and an anesthesia injection cavity arranged adjacent to the curved induction channel, and an anesthesia device is arranged in the anesthesia injection cavity.

3. The multi-channel small animal inhalation anesthesia induction box according to claim 2, characterized in that: The anesthesia device comprises an anesthesia device for anesthetizing small animals and a concentration monitoring mechanism (10) for monitoring the concentration of anesthetic gas in the anesthesia device.

4. The multi-channel small animal inhalation anesthesia induction box according to claim 1, characterized in that: The regulator is arranged on the side wall of the curved induction channel (402), and comprises a channel regulating mechanism (5) and a channel sealing switch mechanism (6); the channel regulating mechanism (5) and the channel sealing switch mechanism (6) are connected via a ventilation duct (502).

5. The multi-channel small animal inhalation anesthesia induction box according to claim 1, characterized in that: The uprighting experimental mechanism (12) comprises an upper uprighting bracket (1201), a lower uprighting bracket (1202), a middle sliding bracket (1203) and a folding enclosure (1207); the middle sliding bracket (1203) is slidably connected to the lower uprighting bracket (1202); the middle sliding bracket (1203) supports the upper uprighting bracket (1201); a first magnet (1206) is installed on one side of the middle sliding bracket (1203); a second magnet (1208) is arranged at a corresponding position of the folding enclosure (1207); a magnetic attraction force is formed between the first magnet (1206) and the second magnet (1208); the middle sliding bracket (1203) moves downward, driving the first magnet (1206) to move downward, causing the first magnet (1206) and the second magnet (1208) to be misaligned, and the helical torsion spring (1204) and the torsion shaft (1219) to rotate synchronously, driving the uprighting experimental mechanism to rotate together, thereby realizing uprighting of the device.

6. The multi-channel small animal inhalation anesthesia induction box according to claim 1, characterized in that: The maze experiment mechanism (13) comprises a cross-shaped maze (1301), a first passageway (1302) and a second passageway (1303). The cross-shaped maze (1301) is a cross-shaped hollow structure, with a first passageway (1302) provided at one end, a folding enclosure (1207) inserted into the open end of the first passageway (1302), and a second passageway (1303) provided at the other end, and the second passageway (1303) is set on the maze experiment mechanism (13).

7. The multi-channel small animal inhalation anesthesia induction box according to claim 1, characterized in that: The balance experiment mechanism (14) comprises an arc-shaped balance lever (1401), a balance spring (1402) and a balance connecting rod (1403); the middle portion of the arc-shaped balance lever (1401) is fixedly connected to the balance connecting rod (1403); one end of the arc-shaped balance lever (1401) is overlapped below the second door (1303) of the cross-shaped maze (1301); and the other end is fixedly connected to the balance spring (1402).

8. The multi-channel small animal inhalation anesthesia induction box according to claim 1, characterized in that: The tail-flick experiment mechanism (15) comprises a restraining part (1505) and a tail-flick experiment part (1504). The restraining part (1505) and the tail-flick experiment part (1504) are arranged adjacent to each other. The valve (1516) of the restraining part (1505) is mounted above the valve mounting hole (1513) by an L-shaped sliding rod (1511). After the L-shaped sliding rod (1511) moves, the valve (1516) falls to restrain and fix the head of the small animal, and the tail-flick experiment part (1504) stimulates the tail of the small animal.

9. A multi-channel small animal inhalation anesthesia induction experimental method, characterized in that: This is achieved using a multi-channel small animal inhalation anesthesia induction box, which includes the following steps: Place the small animal into the induction box body (2), rotate the induction box body (2) so that the induction box base (1) and the induction box body (2) are staggered and sealed, and close the induction box cover (3); After the small animal enters the curved induction channel (402) through the straight induction channel (401), the space of the curved induction channel (402) is regulated by the channel regulating mechanism (5), and the synchronous channel sealing switch mechanism (6) seals the curved induction channel (402) to form an anesthesia device; The concentration of the anesthetic gas in the anesthesia device is monitored in real time by a concentration monitoring mechanism (10), and the concentration of the anesthetic gas in the anesthesia device is adjusted by a control anesthesia device according to experimental needs; After the small animals are anesthetized with different concentrations of anesthetic doses, the uprighting experimental data can be recorded through the uprighting experimental mechanism (12) to test the degree of anesthesia of different concentrations of anesthetic doses and compare the experimental data of the four channels at the same time; After the small animal righting experiment is completed, the time and number of errors of the small animal are measured through the maze test device (13) to determine the effects of different concentrations of anesthetics on the cognitive function of the small animal after anesthesia; After the small animal maze test, the time it takes for the small animal to pass through and the number of errors are measured using a balance test mechanism (14) to determine the effects of different concentrations of anesthetics on the balance of the small animal after anesthesia. After the small animal balance experiment is completed, the small animal's tail is stimulated with electric current through the tail-flicking experiment device (15), and the reaction time of the small animal to quickly flick its tail after feeling pain is recorded to evaluate the animal's sensitivity to pain and pain threshold.

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