A composition with soothing and repairing efficacy and its use

Abstract

The application belongs to the technical field of cosmetics, and particularly relates to a composition with soothing and repairing effects and application thereof in cosmetics. Specifically, the composition comprises the following components in mass fractions: glycerol glucoside 0.01-1 parts, hyaluronic acid disaccharide 0.005-0.2 parts, and sea buckthorn extract 1-12 parts. The soothing and repairing composition is compounded by glycerol glucoside, hyaluronic acid disaccharide and sea buckthorn extract. It is verified by experiments that the composition has the effects of significantly synergistically inhibiting mast cell degranulation and promoting the generation of barrier-related factors DSG1, DSC1 and Ki67, and can be used in cosmetics to improve skin sensitivity problems, and has good stability and good practical application value.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and particularly relates to a composition with soothing and repairing effects and its application in cosmetics. Background Art

[0002] The information disclosed in this background technology section is only intended to enhance understanding of the overall background of the invention and should not necessarily be regarded as an admission or any form of suggestion that the information constitutes the prior art already known to those skilled in the art.

[0003] Sensitive skin is more common in women. Data shows that one in three women has sensitive skin, with those aged 20 to 35 being the most vulnerable, accounting for over 70%. Sensitive skin often manifests as itching, stinging, and burning, and is associated with impaired skin barrier function and neuroimmune disorders. Inflammatory responses mediated by immune cells are a key factor in sensitive skin. Mast cells are immune cells that trigger allergic reactions and participate in host immune defense. When exposed to allergens, mast cells release substances from their granules outside the cell, a process known as mast cell degranulation. Degranulation releases inflammatory mediators such as histamine and leukotrienes, triggering allergic reactions such as itching. Inhibiting mast cell degranulation can effectively alleviate skin allergies such as itching.

[0004] The skin barrier, composed of the "brick wall structure" of the stratum corneum and the tight junctions of the stratum granulosum, is an important foundation for the skin's physiological functions. Damage to the barrier is directly related to the development of sensitive skin. The causes and mechanisms of barrier damage are very complex. Abnormalities in corneodesmosomes can easily lead to excessive desquamation of keratinocytes, which can cause skin barrier defects and subsequent skin inflammation. Desmosomes are the main structures connecting keratinocytes and are composed of oval, dense, and thickened adjacent cell membranes. Desmoglein 1 (DSG1) and desmocollin 1 (DSC1) are the main proteins that make up desmosomes. DSC1 and DSG1 form the electron-transparent intercellular spaces and intercellular contact layers of desmosomes. When cells undergo a cascade reaction, they cause changes in the desmosomal structure, leading to a loss of intercellular adhesion. The normal expression of desmosomal protein structures and the coordinated functions between them can promote the integrity of the epidermal structure and the restoration of barrier function.

[0005] Although there are many soothing and repairing products that hope to solve the problem of skin sensitivity, these products often have relatively simple functions and little effect. Sensitivity often recurs after discontinuation of use. Even if multiple ingredients are stacked in the product or the amount of ingredients added is increased, the expected effect cannot be achieved. Summary of the Invention

[0006] To address the shortcomings of the prior art, the present invention aims to provide a soothing and repairing composition and its application. This soothing and repairing composition is a combination of glycerol glucoside, hyaluronic acid disaccharide, and seabuckthorn extract. Experimental results have shown that this composition significantly synergistically inhibits mast cell degranulation and promotes the production of barrier-related factors DSG1, DSC1, and Ki67. It can be used in cosmetics to improve skin sensitivity and exhibits excellent stability. Based on these research findings, the present invention was completed.

[0007] In order to achieve the above technical objectives, the technical solutions provided by the present invention are as follows:

[0008] The first aspect of the present invention provides a composition having soothing and repairing effects, comprising the following components in parts by weight:

[0009] Glyceryl glucoside 0.01-1 parts, hyaluronic acid disaccharide 0.005-0.2 parts, sea buckthorn extract 1-12 parts.

[0010] The seabuckthorn extract can be obtained by any of the following extraction and preparation methods:

[0011] Extraction method 1 is as follows:

[0012] S1-1, mixing the crushed seabuckthorn fruit with water and performing microwave extraction, performing vacuum decompression reflux extraction on the resulting mixture, and filtering the extract to obtain a filtrate;

[0013] S1-2. The filtrate obtained in step S1 is concentrated under reduced pressure, and ethanol is added, and the mixture is allowed to stand at low temperature. After filtering, the filtrate is concentrated under reduced pressure a second time, 1,3-butanediol is added, and the mixture is stirred and mixed evenly. After filtering, the sea buckthorn extract is obtained.

[0014] Extraction method 2 is as follows:

[0015] S2-1. Take the crushed seabuckthorn fruit, add ethanol aqueous solution and perform reflux extraction, filter the filtrate, repeat the extraction of the residue 1-3 times, and combine the filtrates;

[0016] S2-2. The filtrate obtained in step S1 is concentrated under reduced pressure, 1,3-butanediol is added thereto, the mixture is stirred and mixed evenly, and the sea buckthorn extract is obtained after filtering.

[0017] The second aspect of the present invention provides the use of the above composition in soothing and repairing skin care products.

[0018] The third aspect of the present invention provides a soothing, repairing and skin-care cosmetic, which comprises at least the above-mentioned composition; the cosmetic may be a skin-care cosmetic.

[0019] The fourth aspect of the present invention provides a method for preparing the soothing and repairing skin care cosmetics, which comprises the step of mixing the composition and other raw material components.

[0020] Beneficial technical effects of one or more of the above technical solutions:

[0021] (1) The soothing and repairing composition obtained by the above technical solution has a significant synergistic effect. The soothing and repairing composition obtained by the above technical solution has a significant synergistic effect, which achieves a good soothing and repairing effect by inhibiting mast cell degranulation and promoting the production of barrier-related factors DSG1, DSC1, and Ki67;

[0022] (2) The test results show that sea buckthorn extract ① has better skin care effect than sea buckthorn extract ②, thus making the product more effective.

[0023] (3) Experimental results show that hyaluronic acid disaccharide (400 Da) has better synergistic effect than dimethylsilanol hyaluronate, hydrolyzed sodium hyaluronate (<1W Da), glycerol glucoside, and sea buckthorn extract.

[0024] (4) Sea buckthorn extract is prone to color change, which affects the stability of the product. The stability of the product can be significantly improved by combining it with hyaluronic acid disaccharide (400Da) and glycerol glucoside.

[0025] (5) The preparation method of the soothing and repairing skin care cosmetics obtained by the above technical solution is simple and easy, saves costs, and the raw materials are easy to obtain. It is suitable for mass production and has passed human efficacy experimental tests and has good soothing and repairing effects. Therefore, it has good practical application value.

[0026] At the same time, the cosmetic preparation method obtained by the above technical solution is simple and easy, the raw materials are easy to obtain, and it is suitable for mass production, so it has good practical application value. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are merely embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without any creative work.

[0028] Figure 1 : Histogram of DSC1 immunofluorescence results (## indicates p<0.01 compared with blank control; ** indicates p<0.01 compared with negative control).

[0029] Figure 2: Histogram of DSG1 immunofluorescence results (## indicates p<0.01 compared with blank control; ** indicates p<0.01 compared with negative control).

[0030] Figure 3 : Bar graph of Ki67 immunohistochemistry results (## indicates p<0.01 compared with blank control; ** indicates p<0.01 compared with negative control).

[0031] Figure 4 : Pictures of changes in facial red areas before and after using the sample. DETAILED DESCRIPTION

[0032] It should be noted that the following detailed descriptions are illustrative and intended to provide further explanation of the present invention. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which the present invention belongs.

[0033] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, they indicate the presence of features, steps, operations, devices, components and / or combinations thereof.

[0034] In a typical embodiment of the present invention, a composition with soothing and repairing effects is provided, wherein the composition comprises the following components in parts by weight:

[0035] Glyceryl glucoside 0.01-1 parts, hyaluronic acid disaccharide 0.005-0.2 parts, sea buckthorn extract 1-12 parts.

[0036] In the present invention, the glycerol glucoside is specifically 2-α-glycerol glucoside (2-O-α-D-glucopyranosyl-sn-glycerol, 2-αGG), which has antioxidant activity and high moisturizing ability.

[0037] In the present invention, the hyaluronic acid disaccharide has an INCI name of hydrolyzed sodium hyaluronate and a molecular weight of 400 Da.

[0038] The seabuckthorn extract can be obtained by any of the following extraction and preparation methods; specifically,

[0039] Extraction method 1 is as follows:

[0040] S1-1, mixing the crushed seabuckthorn fruit with water and performing microwave extraction, performing vacuum decompression reflux extraction on the resulting mixture, and filtering the extract to obtain a filtrate;

[0041] S1-2. The filtrate obtained in step S1 is concentrated under reduced pressure, and ethanol is added, and the mixture is allowed to stand at low temperature. After filtering, the filtrate is concentrated under reduced pressure a second time, 1,3-butanediol is added, and the mixture is stirred and mixed evenly. After filtering, the sea buckthorn extract is obtained.

[0042] In step S1-1, the mass ratio of the crushed sea buckthorn fruit to water is 1:15 to 1:20; the microwave power is 300 to 400 W, and the microwave treatment time is 20 to 30 minutes; the extraction temperature of the vacuum decompression reflux extraction is 45 to 60° C., the extraction time is 0.5 to 2 hours, and the number of reflux extractions can be 1 to 3 times. When the number of reflux extractions is greater than 1 time, the extracts obtained each time are combined and then filtered.

[0043] In the step S1-2, the reduced pressure concentration temperature is controlled at 50-70° C., and the reduced pressure concentration is performed to 6-10 times the mass of the crushed sea buckthorn fruit in step S1; ethanol is added until the alcohol content in the obtained concentrated solution is 60-80%; the solution is placed at 4±2° C. and allowed to stand for 10-30 hours; the reduced pressure concentration is performed again to 2-6 times the mass of the crushed sea buckthorn fruit in step S1, and then 1-1.5 times the mass of the obtained secondary concentrated solution is added with 1,3-butanediol.

[0044] The obtained seabuckthorn extract has a concentration of 0.10-0.12 g / g based on the crude drug amount.

[0045] Extraction method 2 is as follows:

[0046] S2-1. Take the crushed seabuckthorn fruit, add ethanol aqueous solution and perform reflux extraction, filter the filtrate, repeat the extraction of the residue 1-3 times, and combine the filtrates;

[0047] S2-2. The filtrate obtained in step S1 is concentrated under reduced pressure, 1,3-butanediol is added thereto, the mixture is stirred and mixed evenly, and the sea buckthorn extract is obtained after filtering.

[0048] Wherein, in the step S2-1, the ethanol concentration in the ethanol aqueous solution is 20-40%, the mass ratio of the crushed sea buckthorn fruit to the ethanol aqueous solution is 1:8-1:12, and the specific conditions of the reflux extraction are: reflux extraction at 60-80° C. for 0.2-1 h;

[0049] In step S2-2, the reduced pressure concentration temperature is controlled at 50-70° C., and the reduced pressure concentration is performed to 2-6 times the mass of the crushed sea buckthorn fruit in step S1; then 1-1.5 times the mass of the concentrate is added with 1,3-butanediol.

[0050] The obtained seabuckthorn extract has a concentration of 0.10-0.12 g / g based on the crude drug amount.

[0051] In another specific embodiment of the present application, the use of the above-mentioned composition in soothing and repairing skin care products is provided. Through experimental verification, the above-mentioned composition can achieve the effects of soothing by inhibiting mast cell degranulation, repairing by promoting the production of barrier-related factors DSG1, DSC1 and Ki67, and is safe and non-toxic, and thus can be applied to soothing and repairing skin care cosmetics. Therefore, the product can be a cosmetic, and further can be a skin care cosmetic (i.e. skin care product). It should be noted that in the present application, the cosmetic refers to a daily-use chemical industry product that is spread on any part of the human body, especially the skin, by rubbing, spraying or other similar methods.

[0052] In another specific embodiment of the present application, a soothing and repairing skin care cosmetic is provided, which comprises at least the above-mentioned composition. The cosmetic can be a skin care cosmetic.

[0053] The cosmetic can further comprise other raw material components allowed to be added in the cosmetic field, including but not limited to emulsifiers, emollients, humectants and thickening agents, etc.

[0054] Meanwhile, the present application can also be used to prepare different cosmetic dosage forms, such as liquid, gel, paste, cream, emulsion, spray, mask, etc. by reasonably adding the above-mentioned raw material components, which are not specifically limited herein.

[0055] In another specific embodiment of the present application, the content of the soothing and repairing composition is 0.5-10.0% based on the total mass of the soothing and repairing skin care cosmetic.

[0056] In another specific embodiment of the present application, the content of the soothing and repairing composition is 2.0-5.0% based on the total mass of the soothing and repairing skin care cosmetic.

[0057] In another specific embodiment of the present application, a preparation method of the above-mentioned soothing and repairing skin care cosmetic is provided, which comprises the step of mixing the composition and other raw material components.

[0058] The present application will be further described below in conjunction with examples. The present application will be further described below by way of examples, but the present application is not limited in the scope of the examples. Based on the examples in the present application, any changes to the present application made by those skilled in the art without creativity shall fall within the protection scope of the present application. The hyaluronic acid disaccharide used in the examples and comparative examples has the trade name of HAF400, the INCI name of sodium hyaluronate, the molecular weight of 400 Da, and is purchased from Shandong Bai Fu Fu Ruida Pharmaceutical Co., Ltd.; the glycerol glucoside is specifically 2-α-glycerol glucoside, which is purchased from Qingdao Zhongke Lan Zhi Biological Technology Development Co., Ltd.; other reagents and materials are ordinary commercially available products unless otherwise specified.

[0059] Examples 1-4 and Comparative Examples 1-9:

[0060] The specific scheme is shown in Table 1 below, calculated by weight. The preparation method is as follows: glycerol glucoside, hyaluronic acid disaccharide, and sea buckthorn extract are mixed together and dissolved in purified water.

[0061] The preparation method of the seabuckthorn extract ① comprises the following specific steps:

[0062] (1) Take an appropriate amount of dried sea buckthorn fruit powder and mix it with water for microwave extraction. The mass ratio of material to liquid is 1:20, the microwave power is 350W, and the microwave time is 30min. The treated mixed solution is subjected to vacuum decompression reflux extraction at an extraction temperature of 50℃ for 60min. Reflux extraction is performed twice, and the two extracts are combined and filtered.

[0063] (2) The filtrate obtained in step (1) was concentrated under reduced pressure at 60°C to 8 times the mass of the sea buckthorn fruit powder, ethanol was added until the alcohol content of the concentrate was 70%, and the concentrate was placed in a 4°C refrigerator for 24 hours, filtered, and the filtrate was concentrated under reduced pressure to about 4.5 times the mass of the sea buckthorn fruit powder. 1,3-butanediol was added in an amount of about 1.1 times the mass of the concentrate, stirred and mixed uniformly, and filtered to obtain sea buckthorn extract ①. The concentration of the extract was 0.1021 g / g based on the crude drug amount.

[0064] The preparation method of the seabuckthorn extract ② comprises the following specific steps:

[0065] (1) Take an appropriate amount of dried sea buckthorn fruit powder, add 40% ethanol aqueous solution, the material-liquid mass ratio is 1:10, reflux extraction at 60°C for 40 minutes, filter the filtrate, repeat the extraction of the filter residue twice, and combine the three filtrates.

[0066] (2) The filtrate obtained in step (1) was concentrated under reduced pressure at 60° C. to a concentration of about 4.5 times the mass of the seabuckthorn fruit powder, and 1,3-butanediol (about 1.1 times the mass of the concentrate) was added. The mixture was stirred and mixed uniformly, and filtered to obtain seabuckthorn extract ②. The concentration of the seabuckthorn extract was 0.1053 g / g based on the crude drug amount.

[0067] Table 1 Specific Examples and Comparative Examples Ingredients

[0068]

[0069]

[0070] Examples 5, 6, and Comparative Examples 10-16: Essence Water

[0071] Preparation method of essence water:

[0072] S1: Add the ingredients in phase A respectively, heat to 85℃ and stir to dissolve;

[0073] S2: After complete dissolution, start to cool down, when the temperature reaches 45℃, add the ingredients in phase B respectively, stir to dissolve and disperse evenly;

[0074] S3: When the temperature reaches 40℃, add the ingredients in phase C respectively, stir to dissolve and disperse evenly, and the product of essence water is obtained.

[0075] Table 2 Formulation of product of essence water

[0076]

[0077] Note: PHL is 1,3-propanediol, 1,2-hexanediol, octanoyl hydroxamic acid, purchased from Jingchenlong Chemical Co., Ltd.

[0078] In example 5, 6 and comparative examples 10-16, phase B in the formulation is added according to table 3.

[0079] Table 3 Summary of raw materials of phase B in example 6 and comparative examples 10-16

[0080]

[0081]

[0082] Test example 1: Stability test

[0083] Example 5, 6 and comparative examples 10-16 are subjected to stability test. Example 5, 6 and comparative examples 10-16 are respectively placed in cold and hot cycle (-18℃, 4℃, 45℃, cycle once every 3 days) for 4 weeks, 45℃ and light for 12 weeks. Observe once every two weeks. When observing, the sample needs to be cooled to room temperature. Check whether the sample has color change phenomenon.

[0084] Table 4 Stability test results

[0085]

[0086] The test results are shown in Table 4. Examples 5 and 6 did not undergo color change under hot and cold cycling, 45°C, and light conditions. Comparative Examples 10, 11, and 13-16 all experienced color change under hot and cold cycling, 45°C, and light conditions. Comparative Example 12 experienced color change under 45°C and light conditions. The results show that sea buckthorn extract ① prepared by method 1 and sea buckthorn extract ② prepared by method 2 are both prone to color change under 45°C and light conditions. Based on the color change time, sea buckthorn extract ① is more stable than sea buckthorn extract ②. Glycerol glucoside and hyaluronic acid disaccharide can significantly improve the color change of sea buckthorn extract when combined with sea buckthorn extract ① and sea buckthorn extract ②. However, when combined with sea buckthorn extract ②, color change still occurred within the test time and failed the stability test. When combined with sea buckthorn extract ①, no color change occurred within the test time and passed the stability test.

[0087] Test Example 2: In vitro soothing efficacy study

[0088] This experiment uses mast cells as experimental subjects. The degranulation model is established by stimulating mast cells with C48 / 80. The soothing effect of the test samples is evaluated based on the morphology and degranulation rate.

[0089] Press 1×10 5 Seed cells into 24-well plates at a seeding density of 100 cells / well and incubate overnight in an incubator (37°C, 5% CO2). Prepare the test substance working solution according to the test protocol in Table 5. When the cell plating rate in the 24-well plate reaches 40%-50%, administer the test substance to each well. Add 1 mL of culture medium containing the test substance at varying concentrations to each well, with three replicates per group. After administration, incubate the 24-well plates in an incubator (37°C, 5% CO2) for 2 hours.

[0090] After incubation for 2 hours, the samples were discarded. Based on the experimental grouping, the blank control group received 1 mL of serum-free, high-glucose DMEM medium; the negative control group received 1 mL of serum-free, high-glucose DMEM medium containing C48 / 80; the positive control group received 1 mL of serum-free, high-glucose DMEM medium containing C48 / 80 and sodium cromoglycate; and the sample group received 1 mL of serum-free, high-glucose DMEM medium containing C48 / 80 and the corresponding sample administration concentration. After administration, C48 / 80 stimulation was performed for 45 minutes, and the reaction was terminated in an ice bath. The inhibition rate was calculated as (OD value of the NC group - OD value of the sample) / OD value of the NC group * 100%.

[0091] After the culture, the degranulation of cells in each group was observed under an inverted microscope, and the degranulation rate of cells was counted and calculated using IPP software.

[0092] Table 5 Test groups

[0093]

[0094]

[0095] The test results are shown in Table 6. Compared with the unstimulated group (BC group), after C48 / 80 stimulation (NC group), the cells showed obvious degranulation morphology (P < 0.01). After treatment with sodium cromoglycate (PC group), the number of degranulated cells in the visual field decreased significantly (P < 0.01), indicating that the C48 / 80-induced mast cell degranulation model was successfully established and the experiment was effective. Compared with the NC group, the number of degranulated cells in the visual field of Examples 1-4 and Comparative Examples 1-9 decreased significantly, showing a significant inhibitory effect on mast cell degranulation. Examples 2 and 3 had the most significant inhibitory effect. The inhibition rate of the examples was higher than that of the comparative examples, indicating that the synergistic effect of the sea buckthorn extract extracted by method 1 was more significant; hyaluronic acid disaccharide was more significant than dimethylsilanol hyaluronate, hydrolyzed sodium hyaluronate (<1W Da) and glycerol glucoside, and sea buckthorn extract; the soothing repair composition can synergistically inhibit mast cell degranulation, thereby achieving a soothing effect.

[0096] Table 6 Summary of mast cell degranulation rates

[0097]

[0098] (Note: ## indicates p < 0.01 compared with the blank control; ** indicates p < 0.01 compared with the negative control.)

[0099] Experimental Example 3: In vitro repair efficacy study

[0100] This test uses a 3D epidermal skin model To prepare the 0.1% SLS working solution, pipette 1 mL of 0.2% SLS solution and add 1 mL of PBS to make a 0.1% SLS working solution. To prepare the positive control group (WY14643) working solution, pipette 10 μL of 30 mM WY14643 stock solution into 6 mL of culture medium to make 50 μM WY14643.

[0101] According to the test grouping in Table 7, the model was transferred to a 6-well plate (0.9mL EpiGrowth culture medium was added in advance), and the test group number was marked on the 6-well plate. 25μL of 0.1% SLS solution was added to the surface of the negative control group, the positive control group, Examples 1-4, and Comparative Examples 1-6, and incubated for 30 minutes. After the incubation, the positive control group added a working solution of the corresponding concentration, took 12.5μL of the sample to be tested and added it to the surface of the model. After evenly coating, it was placed in a CO2 incubator (37°C, 5% CO2) and incubated for 24 hours. After the incubation was completed, the test substance remaining on the surface of the model was cleaned with sterile PBS solution, and the residual liquid inside and outside the model was wiped off with a sterile cotton swab.

[0102] Immunofluorescence test: take the model for detection, cut off the model ring, fix with 4% paraformaldehyde, after 24h fixation, detect the content of DSC1 and DSG1 by immunofluorescence, observe under microscope, collect pictures and analyze.

[0103] Immunohistochemical test: take the model for detection, cut off the model ring, fix with 4% paraformaldehyde, after 24h fixation, detect the content of Ki67 by immunohistochemistry, observe under microscope, collect pictures and analyze.

[0104] Table 7 test grouping

[0105]

[0106] The test results of immunofluorescence test are shown in Figure 1 , Figure 2 .

[0107] Compared with the BC group, the DSC1 content of the NC group decreased significantly, indicating that the stimulation condition of this test was effective. Compared with the NC group, the DSC1 content of the PC group increased significantly, with an increase rate of 155.17%, indicating that the positive control of this test was effective. Compared with the NC group, the DSC1 content of the groups of examples 1-4 and comparative examples 1-6 increased significantly, with an increase rate of 110.34%, 165.52%, 196.55, 144.83%, 55.17%, 34.48%, 51.72%, 58.62%, 48.28%, 55.17% respectively, and examples 2 and 3 had the most significant promoting effect, the increase rate of examples was higher than that of comparative examples, indicating that the synergistic effect of seabuckthorn extract extracted by method one was more significant; the synergistic effect of hyaluronic acid disaccharide, dimethylsilyl hyaluronate, hydrolyzed sodium hyaluronate (<1W Da), glycerol glucoside and seabuckthorn extract was more significant; the soothing and repairing composition can synergistically promote the expression of DSC1.

[0108] Compared with the BC group, the DSG1 content of the NC group decreased significantly, indicating that the test stimulation condition was effective. Compared with the NC group, the DSG1 content of the PC group increased significantly by 196.88%, indicating that the positive control of this test was effective. Compared with the NC group, the DSG1 content of the groups of Examples 1-4 and Comparative Examples 1-6 increased significantly by 187.50%, 278.13%, 318.75, 228.13%, 112.50%, 68.75%, 90.63%, 128.13%, 96.88%, and 87.50%, respectively, and the promotion effects of Examples 2 and 3 were the most significant, the promotion rates of the examples were higher than those of the comparative examples, indicating that the seabuckthorn extract extracted by Method One had a more significant synergistic effect; the synergistic effect of hyaluronic acid disaccharide was more significant than that of dimethylsilanol hyaluronate, hydrolyzed sodium hyaluronate (<1W Da), glycerol glucoside, and seabuckthorn extract; and the soothing and repairing composition can synergistically promote the expression of DSG1.

[0109] The results of the immunohistochemical test are shown in Table 2. Figure 3

[0110] Compared with the BC group, the Ki67 content of the NC group decreased significantly, indicating that the test stimulation condition was effective. Compared with the NC group, the Ki67 content of the PC group increased significantly by 451.04%, indicating that the positive control of this test was effective. Compared with the NC group, the Ki67 content of the groups of Examples 1-4 and Comparative Examples 1-6 increased significantly by 227.59%, 312.45%, 388.59%, 288.38%, 122.41%, 59.96%, 93.98%, 140.87%, 95.23%, and 98.13%, respectively, and the promotion effects of Examples 2 and 3 were the most significant, the promotion rates of the examples were higher than those of the comparative examples, indicating that the seabuckthorn extract extracted by Method One had a more significant synergistic effect; the synergistic effect of hyaluronic acid disaccharide was more significant than that of dimethylsilanol hyaluronate, hydrolyzed sodium hyaluronate (<1W Da), glycerol glucoside, and seabuckthorn extract; and the soothing and repairing composition can synergistically promote the expression of Ki67.

[0111] ​Test Example 4: Test results of the experiment on soothing and repairing effects of cosmetics containing the skin care composition of the present invention on human body The prepared essence water product of Example 5 was subjected to human skin efficacy test, and 30 volunteers aged 18-40 (skin condition: sensitive skin (lactic acid stinging positive)) were recruited for product testing. If the a* value of the red zone analysis at any visit time point after the use of the sample is significantly reduced compared with that before use (P<0.05), the product is considered to have the effect of soothing the skin, and is used to evaluate its soothing effect on the human face. If the transepidermal water loss, TEWL, at any visit time point after the use of the sample is significantly reduced compared with that before use (P<0.05), the product is considered to have the effect of repairing the skin barrier function, and is used to evaluate its repair effect on the human face.

[0112] Test environment: temperature: 20.1℃~21.8℃, relative humidity: 44%~50%;

[0113] Testing time: before using the product, 2 weeks after using the sample, and 4 weeks after using the sample;

[0114] Testing equipment: VISIA skin tester (CanField, USA); skin water loss tester AquaFlux (AF200, BIOX, UK).

[0115] Facial Red Zone Test: Using a VISIA skin analyzer, images of the left, center, and right sides of the subject's face were collected under three light sources: Standard Light 1, Standard Light 2, and cross-polarized light. Red zone images were then generated under cross-polarized light. Image analysis of the red zone images yielded a quantitative indicator, the red zone analysis a* value, which was used to assess improvements in facial redness and sensitivity.

[0116] Transepidermal water loss (TEWL) test: The skin water loss tester Aqua Flux is used. The instrument uses a single silicon crystal chip as a water vapor temperature and humidity sensor to measure the flow density of water vapor and then determine the transepidermal water loss of the skin.

[0117] Calculation formula:

[0118] SPSS Statistics 25 was used for statistical analysis, with two-tailed tests and an α level of 0.05.

[0119] The measurement data were analyzed using the normal distribution test results. If the measured values ​​were normally distributed, the t-test method was used for statistical analysis; if they were non-normally distributed, the rank sum test method was used for statistical analysis.

[0120] The results of the facial red zone test are shown in Tables 8 and 9:

[0121] like Figure 4The following are pictures showing the changes in facial red area of ​​2 subjects before and after using the samples.

[0122] Table 8 Descriptive statistics of a* value in red zone analysis (n=30)

[0123]

[0124] Table 9 Statistical analysis results of a* value in red zone analysis (n=30)

[0125]

[0126] Statistical analysis results: “-”: the difference is not statistically significant (P≥0.05); “*”: the difference is statistically significant (0.01≤P<0.05); “**”: the difference is statistically significant (0.001≤P<0.01); “***”: the difference is statistically significant (P<0.001).

[0127] The red zone analysis a* value test results showed that compared with before use, the red zone analysis a* value was significantly reduced after 2 weeks of use of the test sample (0.001≤P<0.01), and the red zone analysis a* value was significantly reduced after 4 weeks of use of the test sample (P<0.001), indicating that the product containing the soothing and repairing skin care composition has a soothing effect.

[0128] The transepidermal water loss TEWL test results are shown in Tables 10 and 11:

[0129] Table 10 Descriptive statistics of transepidermal water loss (TEWL) values ​​(n=30)

[0130]

[0131] Table 11 Statistical analysis results of transepidermal water loss (TEWL) values ​​(n=30)

[0132]

[0133] (Note: Statistical analysis results: “-”: no statistically significant difference (P ≥ 0.05); “*”: statistically significant difference (0.01 ≤ P < 0.05); “**”: statistically significant difference (0.001 ≤ P < 0.01); “***”: statistically significant difference (P < 0.001).)

[0134] The test results showed that after using cosmetics containing 3.0% soothing and repairing composition for 2 weeks and 4 weeks, the transepidermal water loss (TEWL) of the skin was significantly reduced, decreasing by 17.87% and 25.29% respectively compared with before use, indicating that cosmetics containing soothing and repairing skin care composition have a repairing effect.

[0135] It should be noted that the above examples are only used to illustrate the technical solutions of the present application but not to limit the present application. Although the present application is explained in detail with reference to the examples, the technical solutions of the present application can be modified or equivalently replaced by those skilled in the art according to the technical solutions of the present application without departing from the spirit and scope of the technical solutions of the present application.

Claims

1. A composition with soothing and repairing effects, characterized in that: The composition comprises the following components in parts by weight: 0.01-1 parts of glyceryl glucoside, 0.005-0.2 parts of hyaluronic acid disaccharide, 1-12 parts of sea buckthorn extract; The seabuckthorn extract is obtained by the following extraction and preparation method; specifically, The extraction method is as follows: S1-1, mixing the crushed seabuckthorn fruit with water and performing microwave extraction, performing vacuum decompression reflux extraction on the resulting mixture, and filtering the extract to obtain a filtrate; S1-2, concentrating the filtrate obtained in step S1-1 under reduced pressure, adding ethanol, and standing at low temperature. After filtering, the filtrate is concentrated under reduced pressure a second time, adding 1,3-butanediol, stirring and mixing, and filtering to obtain the sea buckthorn extract; In step S1-1, the mass ratio of the crushed sea buckthorn fruit to water is 1:15-1:20; the microwave power is 300-400W, and the microwave treatment time is 20-30min; the extraction temperature of the vacuum decompression reflux extraction is 45-60°C, the extraction time is 0.5-2h, and the number of reflux extractions is 1-3 times. When the number of reflux extractions is greater than 1 time, the extracts obtained each time are combined and then filtered; In the step S1-2, the reduced pressure concentration temperature is controlled at 50-70° C., and the reduced pressure concentration is performed to 6-10 times the mass of the crushed sea buckthorn fruits in step S1-1; ethanol is added until the alcohol content of the obtained concentrated solution is 60-80%; the solution is placed at 4±2° C. and allowed to stand for 10-30 hours; the reduced pressure concentration is performed a second time to 2-6 times the mass of the crushed sea buckthorn fruits in step S1-1, and then 1-1.5 times the mass of the obtained secondary concentrated solution is added with 1,3-butanediol.

2. Use of the composition according to claim 1 in soothing, repairing and skin care products.

3. The use according to claim 2, characterized in that The product is a cosmetic.

4. The use according to claim 3, characterized in that The cosmetics are skin care cosmetics.

5. A soothing and repairing skin care cosmetic, characterized in that: The cosmetics at least comprise the composition according to claim 1; the cosmetics are skin care cosmetics.

6. A soothing and repairing skin care cosmetic according to claim 5, characterized in that: Based on the total mass of the soothing and repairing skin care cosmetic, the amount of the composition added is 0.5-10.0%.

7. A soothing and repairing skin care cosmetic according to claim 6, characterized in that: Based on the total mass of the soothing and repairing skin care cosmetic, the amount of the composition added is 2.0-5.0%.

8. The method for preparing the soothing and repairing skin care cosmetic according to claim 5, characterized in that: The preparation method comprises the step of mixing the composition according to claim 1 and other raw material components.

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