Oil-in-water composition containing ceramides and preparation method and use thereof

Abstract

The embodiment of the present application discloses an oil-in-water composition containing ceramides, and a preparation method and use thereof, which can reduce the phenomenon of crystallization. The oil-in-water composition containing ceramides includes: ceramides, wherein the ceramides are selected from ceramides or ceramide analogs; oil; an emulsifier including sucrose polystearate and sodium di(laurylamide glutamine) lysine; and water; wherein the content of the ceramides is 0.1-10%; the content of the emulsifier is 0.2-6.3%; the mass ratio of the sucrose polystearate to the sodium di(laurylamide glutamine) lysine is 5-60:1; the oil-in-water composition containing ceramides is formed by emulsifying the ceramides, the emulsifier, the oil and the water.

Description

Technical Field

[0001] The present invention relates to the technical field of oil-in-water compositions, and in particular to an oil-in-water composition containing ceramides, a preparation method thereof and uses thereof. Background Art

[0002] Ceramides have relatively low solubility in water and oil. Usually, when added as raw material components to an oil-in-water composition, they tend to precipitate in the form of crystals, resulting in major defects in the stability of the oil-in-water composition. Summary of the invention

[0003] One purpose of the embodiments of the present application is to solve at least one problem in the above background and provide corresponding beneficial effects.

[0004] Another object of the embodiments of the present application is to provide an oil-in-water composition and a preparation method thereof, which can reduce the phenomenon of ceramide substances precipitating in the form of crystals, thereby improving the stability of the oil-in-water composition.

[0005] Another purpose of the embodiments of the present application is to provide a use of an oil-in-water composition.

[0006] The embodiments of the present application mainly achieve the above objectives through the following technical solutions.

[0007] In one aspect, the present invention provides an oil-in-water composition, comprising:

[0008] Ceramides, wherein the ceramides are selected from ceramides (Cers) or ceramide analogs;

[0009] Oil;

[0010] an emulsifier comprising sucrose polystearate and sodium di(laurylamidoglutamine) lysine; and

[0011] water.

[0012] In some technical solutions, the content of ceramides is 0.1-10%.

[0013] In some technical solutions, the content of the emulsifier is 0.2% to 6%.

[0014] In some technical schemes, in the emulsifier, the mass ratio of the sucrose polystearate to the sodium di(laurylamide glutamine) lysine is 5 to 60:1.

[0015] In some technical solutions, the content of sucrose polystearate is 0.1-6%.

[0016] The content of sodium di(lauroyl glutamine) lysine is 0.01% to 0.3%.

[0017] In some technical solutions, the oil includes at least one of vegetable oil, non-polar hydrocarbon oil, and synthetic ester oil.

[0018] In some technical schemes, the oil is selected from one or more of neopentyl glycol dicaprylate / dicaprate, pentaerythritol tetra(ethylhexanoate), olive oil, sunflower seed oil, squalane, mineral oil, dibutyl adipate, and coconut oil caprylate / caprate.

[0019] In some technical solutions, the ceramide is selected from one or more of ceramide EOP, ceramide AS, ceramide AP, ceramide NS / ceramide NG, and ceramide NP;

[0020] The ceramide analog is selected from one or more of cetyl-PG hydroxyethyl palmitamide, hydroxyethyl palmitoxy hydroxypropyl palmitamide, dihydrosphingosine, hexanoylsphingosine, hydroxypalmitoyl dihydrosphingosine, salicylphytosphingosine, tetraacetylphytosphingosine, acetylphytosphingosine, and phytosphingosine.

[0021] In some technical schemes, the oil phase formed by the ceramides, the mixed emulsifier, and the oily substance, and the water phase formed by the water, after emulsification, form the water-in-oil composition containing the amides.

[0022] In some technical solutions, the oily substance also includes oil-soluble components, and the aqueous liquid also includes water-soluble components.

[0023] In another aspect, the present invention also provides a method for preparing an oil-in-water composition, comprising:

[0024] heating water to a temperature of 75 to 85° C. to obtain an aqueous phase;

[0025] The emulsifier, ceramides and oil are mixed and heated to 80-95° C., and then stirred to obtain an oil phase; wherein the ceramides are selected from ceramides or ceramide analogs;

[0026] At a temperature of 75 to 85° C., the oil phase and the water phase are mixed and homogenized for 2 to 5 minutes at a homogenization speed of 1000 to 3000 rpm to obtain a mixed phase;

[0027] The mixed phase is stirred continuously at a stirring speed of 15 to 60 rpm / min until the temperature is no higher than 45° C. to obtain an oil-in-water composition containing amides.

[0028] In some technical solutions, according to the method for preparing the oil-in-water composition,

[0029] The ceramide is selected from one or more of ceramide EOP, ceramide AS, ceramide AP, ceramide NS / ceramide NG, and ceramide NP;

[0030] The ceramide analog is selected from one or more of cetyl-PG hydroxyethyl palmitamide, hydroxyethyl palmitoxy hydroxypropyl palmitamide, dihydrosphingosine, caproylsphingosine, hydroxypalmitoyl dihydrosphingosine, salicyloyl phytosphingosine, tetraacetyl phytosphingosine, acetyl phytosphingosine, and phytosphingosine; the oil is selected from one or more of neopentyl glycol dicaprylate / dicaprate, pentaerythritol tetra(ethylhexanoate), olive oil, sunflower seed oil, squalane, mineral oil, dibutyl adipate, and coconut oil alcohol-caprylate / caprate;

[0031] The content of the ceramides is 0.1 to 10%;

[0032] The content of the emulsifier is 0.2% to 6%;

[0033] In the emulsifier, the mass ratio of the sucrose polystearate to the sodium di(lauroyl glutamine) lysine is 5 to 60:1;

[0034] The content of sucrose polystearate is 0.1-6%,

[0035] The content of sodium di(lauroyl glutamine) lysine is 0.01% to 0.3%.

[0036] In yet another aspect, the embodiments of the present application further provide an oil-in-water composition prepared according to the aforementioned method for preparing the oil-in-water composition.

[0037] In yet another aspect, the embodiments of the present application also provide a use of the oil-in-water composition in preparing a skin care product.

[0038] The beneficial effects of the embodiments of the present invention include:

[0039] 1. In some embodiments, the ceramide-containing oil-in-water composition provided in the embodiments of the present application, due to the use of a specific mixed emulsifier, can reduce the phenomenon of ceramide crystallization and effectively improve the stability of the oil-in-water composition, thereby overcoming the defects mentioned in the background technology.

[0040] 2. In some embodiments, the ceramide-containing oil-in-water composition provided in the embodiments of the present application contains ceramides, so it can be used as a skin care product to provide ceramide ingredients to the skin and play a role in protecting the skin.

[0041] 3. If the oil-in-water composition has the defect of a large amount of crystallization of ceramide components, using this oil-in-water composition for skin care is likely to cause or increase damage to the stratum corneum of the skin. It is understood that in some embodiments, the oil-in-water composition containing ceramides provided in the embodiments of the present application can reduce the phenomenon of crystallization of ceramides, so when used as a skin care product, it can also reduce or avoid damage to the skin.

[0042] 4. In some embodiments, the ceramide-containing oil-in-water composition of the embodiments of the present application contains a specific mixed emulsifier, which can improve the performance of the oil-in-water composition, and can also effectively reduce the transepidermal water loss of the skin and improve the moisturizing effect. Therefore, it can be used to prepare moisturizing skin care products with better moisturizing effect.

[0043] 5. In some embodiments, the ceramide-containing oil-in-water composition of the embodiments of the present application can improve the performance of the oil-in-water composition due to the presence of a specific mixed emulsifier, and can also effectively reduce the transepidermal water loss of the skin in a low-temperature environment and improve the moisturizing effect. Therefore, it can be used to prepare moisturizing skin care products with better moisturizing effect and more suitable for low-temperature environments. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 This is a schematic diagram of the structure of the oil-in-water composition of Example 1 of the present application at 3.5 months at room temperature;

[0045] Figure 2 A schematic diagram of the structure of the oil-in-water composition of Comparative Example 1-2 at room temperature at 3.5 months;

[0046] Figure 3 A schematic diagram of the structure of the oil-in-water composition of Comparative Example 1-1 at room temperature at 3.5 months;

[0047] Figure 4 A schematic diagram of the structure of the oil-in-water composition of Comparative Examples 1-7 at room temperature at 3.5 months;

[0048] Figure 5 A schematic diagram showing a comparison of moisture distribution images of the experimental group in the initial state and the state after the test in the transdermal moisture control test in the embodiment of the present application is shown;

[0049] Figure 6 A schematic diagram showing a comparison of moisture distribution images of the control group in the transdermal moisture control test in the embodiment of the present application in the initial state and the state after the test. DETAILED DESCRIPTION

[0050] The present invention is further described in detail below in conjunction with the accompanying drawings so that those skilled in the art can implement the invention with reference to the description.

[0051] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0052] The terms "first", "second", etc. in the embodiments of the present application are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first", "second", etc. may explicitly or implicitly include at least one of the features.

[0053] Furthermore, the terms "comprises," "comprising," "including," "having," and any variations thereof are intended to cover non-exclusive inclusions. For example, a process, method, system, product, or apparatus comprising a series of steps or units is not limited to the listed steps or units, but may optionally include steps or units not listed, or may optionally include other steps or units inherent to the process, method, product, or apparatus.

[0054] <Amide-containing oil-in-water composition>

[0055] In a first aspect, the present invention provides an oil-in-water composition containing ceramides, comprising:

[0056] Ceramides, wherein the ceramides are selected from ceramide or ceramide analogs;

[0057] Oil;

[0058] an emulsifier comprising sucrose polystearate and sodium di(laurylamidoglutamine) lysine; and

[0059] water.

[0060] The oil-in-water composition can usually be formed by emulsifying the oil phase (oil-containing liquid) and the water phase (water-containing liquid). Emulsification is a phenomenon in which a liquid is uniformly dispersed in another immiscible liquid in the form of particles (droplets or liquid crystals); two immiscible liquids, such as oil and water, are separated into two layers in a container. If an emulsifier is added and stirred, the oil is dispersed in the water to form an emulsion. This process is called emulsification. Therefore, it can be understood that after knowing the above formula components of the oil-in-water composition containing ceramides in the embodiment of the present application, those skilled in the art can form the oil-in-water composition containing ceramides by conventional emulsification means without creative labor. Exemplarily, the oil phase formed by mixing the ceramides, the emulsifier, and the oily substance and the water phase formed by the water are emulsified to obtain the oil-in-water composition containing ceramides.

[0061] In the oil-in-water composition containing ceramides provided in the embodiments of the present application, the emulsifier comprises sucrose polystearate and sodium di(laurylamide glutamine) lysine, which is a specific mixed emulsifier. Therefore, it is possible to reduce the crystallization of ceramides and effectively improve the stability of the oil-in-water composition, thereby overcoming the defects mentioned in the background technology.

[0062] In addition, ceramide is the main component of the intercellular lipids in the stratum corneum, which can not only form a waterproof barrier and help the skin lock in moisture, but also help to repair the barrier and protect the skin from external stimuli. On the contrary, the lack of ceramide will lead to problems such as dry skin and aging. The ceramide-containing oil-in-water composition provided in the embodiment of the present application contains ceramides, so when used as a skin care product, it can provide ceramide substances to the skin and play a role in protecting the skin.

[0063] In addition, if the oil-in-water composition has the defect of a large amount of crystallization of ceramide substances, using such an oil-in-water composition for skin care is likely to cause or increase damage to the stratum corneum of the skin. It is understandable that the oil-in-water composition containing ceramides provided in the embodiments of the present application can reduce the phenomenon of crystallization of ceramides, so when used as a skin care product, it can also reduce or avoid damage to the skin.

[0064] Furthermore, in a low-temperature environment, the transepidermal water loss of the skin is usually aggravated; for example, in a cold and dry environment, the moisture content in the air is low, which easily leads to the evaporation of moisture on the skin surface, making the skin dry; in addition, the stratum corneum of the skin is more easily damaged in a cold environment, which hinders the skin's moisturizing function, thereby aggravating the transepidermal water loss of the skin. Therefore, in moisturizing skin care products, how to reduce transepidermal water loss is the key to product development. The ceramide-containing oil-in-water composition of the embodiment of the present application contains a specific mixed emulsifier, which improves the performance of the oil-in-water composition, and can also effectively reduce the transepidermal water loss of the skin in a low-temperature environment and improve the moisturizing effect, so it can be used to prepare moisturizing skin care products with better moisturizing effect.

[0065] In some embodiments, the ceramide is selected from one or more of ceramide EOP, ceramide AS, ceramide AP, ceramide NS / ceramide NG, and ceramide NP.

[0066] Ceramide analogs are compounds with structures similar to ceramide and can play at least partially the same function as ceramide.

[0067] In some embodiments, the ceramide analog is selected from one or more of cetyl-PG hydroxyethyl palmitamide, hydroxyethyl palmitoxy hydroxypropyl palmitamide, dihydrosphingosine, hexanoylsphingosine, hydroxypalmitoyl dihydrosphingosine, salicylphytosphingosine, tetraacetylphytosphingosine, acetylphytosphingosine, and phytosphingosine.

[0068] In some embodiments, the oil (ie, fat) may include one or more of vegetable fat, non-polar hydrocarbon oil, and synthetic ester oil.

[0069] Exemplarily, the vegetable oil can be selected from but not limited to at least one of the following group of substances: linseed oil, camellia oil, macadamia nut oil, corn oil, olive oil, avocado oil, camellia oil, castor oil, safflower oil, cinnamon oil, grape oil, almond oil, rapeseed oil, liquid paraffin, squalane, sunflower oil, wheat germ oil, rice germ oil, rice bran oil, cottonseed oil, soybean oil, peanut oil, tea oil, evening primrose oil, shea butter, and cocoa butter.

[0070] Optionally, the non-polar hydrocarbon oil may be selected from at least one of the following group of substances, but not limited to: hydrogenated polyisobutene, squalane, isoparaffin, hydrogenated polydecene, liquid paraffin, petrolatum, vaseline, microcrystalline wax.

[0071] For the synthetic ester oil, optionally, at least one of the following group of substances can be selected, but not limited to: phytosterol / octyldodecyl lauroyl glutamate, tri(ethylhexanoin)glyceryl, caprylic / capric triglyceride, isopropyl palmitate, dicaprylyl carbonate, ethylhexyl hydrogenated olive oil, isopropyl myristate, pentaerythritol tetra(ethylhexanoate), isononyl isononanoate, neopentyl glycol diheptanoate, palmitic acid esters such as octyl palmitate, isocetyl stearate, isopropyl myristate, octyldodecyl myristate, caprylic acid esters such as cetyl caprylate, tri-2-ethylhexanoin glyceryl, tetra-2-ethylhexanoic acid neopentyl tetradecyl, diisopropyl adipate, diethyl sebacate, diester of adipate, diester of sebacate, polyoxybutylene polyoxypropylene glycol, polydimethylsiloxane, coconut oil alcohol-caprylate / caprate.

[0072] In some embodiments, the oil is selected from one or more of neopentyl glycol dicaprylate / dicaprate, pentaerythritol tetra(ethylhexanoate), olive oil, sunflower seed oil, squalane, mineral oil, dibutyl adipate, and coconut oil caprylate / caprate.

[0073] In some embodiments, the ceramide-containing oil-in-water composition further comprises a water-soluble component, and the water-soluble component comprises at least one of a first active ingredient, a first preservative, a first thickener, and a first chelating agent.

[0074] Exemplarily, the first active ingredient includes at least one of a first antioxidant, a first free radical scavenger, a first wetting agent, a first moisturizing agent, a first bleaching agent, a first whitening agent, a first fat regulating agent, a first anti-acne agent, a first hair growth agent, a first anti-dandruff agent, a first anti-aging agent, a first anti-wrinkle agent, a first anti-photoaging agent, a first skin softener, a first anti-inflammatory agent, a first cooling agent, a first antibacterial agent, a first antifungal agent, a first antiperspirant, a first deodorant, a first fragrance, a first sunscreen, a first ultraviolet absorber, a first immune enhancer, and a first nutrient.

[0075] In some embodiments, the ceramide-containing oil-in-water composition further comprises an oil-soluble component, and the oil-soluble component comprises at least one of a second active ingredient, a second preservative, and a second thickener.

[0076] Exemplarily, the second active ingredient includes at least one of a second antioxidant, a second free radical scavenger, a second wetting agent, a second moisturizing agent, a second bleaching agent, a second whitening agent, a second fat regulating agent, a second anti-acne agent, a second hair growth agent, a second anti-dandruff agent, a second anti-aging agent, a second anti-wrinkle agent, a second anti-photoaging agent, a second skin softener, a second anti-inflammatory agent, a second cooling agent, a second antibacterial agent, a second antifungal agent, a second antiperspirant, a second deodorant, a second fragrance, a second sunscreen, a second ultraviolet absorber, a second immune enhancer, and a second nutrient.

[0077] In some embodiments, the mass content of the ceramides is 0.1-10%, optionally 1-3%.

[0078] In some embodiments, the mass content of the emulsifier is 0.2% to 6.3%, optionally 0.6 to 6.1%, optionally 0.6 to 2.1%, optionally 2.1 to 6.1%.

[0079] In some embodiments, in the emulsifier, the mass ratio of the sucrose polystearate to the sodium di(lauroyl glutamine) lysine is 5 to 60:1; optionally, the mass ratio is 5 to 20:1; optionally, the mass ratio is 20 to 60:1; optionally, the mass ratio is 20:1.

[0080] In some embodiments, the mass content of the sucrose polystearate is 0.1-6%, optionally 0.5-6%, optionally 0.5-2%, optionally 2-6%.

[0081] In some embodiments, the mass content of sodium di(laurylamide glutamine) lysine is 0.01% to 0.3%, optionally 0.1%.

[0082] In some embodiments, the mass content of the oil is 0.5-30%, optionally 10-15%.

[0083] In some embodiments, the sum of the mass contents of the oil and the oil-soluble component is 0.5-30%.

[0084] In some embodiments, the mass content of the oil-soluble component is 0-10%, optionally 0.1-10%.

[0085] In some embodiments, based on 100% of the total weight, in the oil-in-water composition containing ceramides, the water content is the balance.

[0086] In some embodiments, the water content is 50-95% by mass.

[0087] In some embodiments, the sum of the water and the water-soluble components is 50-95% by weight.

[0088] In some embodiments, the mass content of the water-soluble component is 0 to 20%, optionally 0.1 to 20%.

[0089] <Method for preparing oil-in-water composition containing ceramides>

[0090] In a second aspect, the present application provides a method for preparing an oil-in-water composition containing ceramides, which can be used to prepare the oil-in-water composition containing ceramides described in the first aspect, and the preparation method comprises the following steps S1 to S4:

[0091] S1. heating water to a temperature of 75 to 85° C. to obtain an aqueous phase;

[0092] S2, mixing the emulsifier, ceramides and oil and heating to 80-95° C., stirring to obtain an oil phase;

[0093] S3, at a temperature of 75 to 85° C., mixing the oil phase and the water phase and homogenizing for 2 to 5 minutes at a homogenizing speed of 1000 to 3000 rpm to obtain a mixed phase;

[0094] S4. Continue stirring the mixed phase at a stirring speed of 15 to 60 rpm / min until the temperature is no higher than 45° C. to obtain an amide-containing oil-in-water composition.

[0095] The preparation method provided in the second aspect can be used to prepare a liquid crystal oil-in-water composition containing ceramides, which is beneficial to improving the stability of the oil-in-water composition.

[0096] In some embodiments, the step of heating water to a temperature of 75 to 85° C. to obtain an aqueous phase comprises:

[0097] The water and water-soluble ingredients are heated to a temperature of 75 to 85°C.

[0098] In some embodiments, the emulsifier, ceramides and oil are mixed and heated to 80-95° C., and then stirred to obtain an oil phase; comprising:

[0099] The emulsifier, ceramides, oil and oil-soluble ingredients are mixed and heated to 80-95° C., and then stirred to obtain an oil phase.

[0100] In some embodiments, S4 specifically comprises: continuing to stir the mixed phase at a stirring speed of 15 to 60 rpm / min until the temperature reaches 35 to 45° C., to obtain an amide-containing oil-in-water composition.

[0101] In some embodiments, the mass content of the ceramides is 0.1-10%.

[0102] In some embodiments, the mass content of the mixed emulsifier is 0.2% to 6%.

[0103] In some embodiments, in the mixed emulsifier, the mass ratio of the sucrose polystearate to the sodium di(laurylamide glutamine) lysine is 5 to 60:1.

[0104] In some embodiments, the mass content of the sucrose polystearate is 0.1% to 6%, and the mass content of the sodium di(laurylamide glutamine) lysine is 0.01% to 0.3%.

[0105] In some embodiments, the mass content of the oily substance is 0.5-30%.

[0106] In some embodiments, the mass content of the oil-soluble component is 0.1-10%.

[0107] In some embodiments, the water content is 50-95% by mass.

[0108] In some embodiments, the mass content of the water-soluble component is 0.1-20%.

[0109] <Uses of oil-in-water compositions containing ceramides>

[0110] It can be understood that according to the ceramide-containing oil-in-water composition provided in the aforementioned embodiments, in certain embodiments, the embodiments of the present application also provide a use of the ceramide-containing oil-in-water composition in preparing a skin care product (ie, skin care product).

[0111] It is understood that, in some embodiments, the water-in-oil composition containing ceramides can be used directly as a skin care product; in certain embodiments, other ingredients (such as moisturizing ingredients, whitening ingredients, etc.) can be added as needed to the water-in-oil composition containing ceramides to form the required skin care product.

[0112] Optionally, the skin care product is a moisturizing skin care product having a moisturizing function.

[0113] <Preparation Example of Oil-in-Water Composition>

[0114] <Examples 1 to 33>

[0115] Example 1

[0116] Preparation of oil-in-water compositions

[0117] Combined with the formulation of Example 1 in Table 1, and according to the preparation method S100, a corresponding oil-in-water composition is prepared.

[0118] The preparation method S100 comprises the following steps:

[0119] S101, heating water to 85° C. to obtain an aqueous phase;

[0120] S102, mixing an emulsifier, ceramides and oil, heating the mixture to 95° C., and stirring the mixture to obtain an oil phase;

[0121] S103, mixing the oil phase and the water phase at a temperature of 85° C. and homogenizing for 5 min at a homogenizing speed of 2000 rpm to obtain a mixed phase;

[0122] S104, continue stirring the mixed phase at a stirring speed of 30 rpm / min until the temperature reaches 45°C to obtain an oil-in-water composition.

[0123] Embodiments 2 to 33

[0124] It is basically the same as Example 1, except that the formula of the oil-in-water composition is different, as shown in Tables 1 to 6.

[0125] Table 1. Formulations of Examples 1 to 7

[0126]

[0127] Table 2, formulations of Examples 8 to 13

[0128]

[0129] Table 3, formulations of Examples 14 to 19

[0130]

[0131] Table 4, formulations of Examples 20 to 25

[0132]

[0133] Table 5. Formulations of Examples 26 to 27

[0134]

[0135] Table 6, formulations of Examples 28 to 33

[0136]

[0137] <Examples 34-35>

[0138] Embodiment 34

[0139] Preparation of oil-in-water compositions

[0140] The method is basically the same as Example 1, except that the preparation conditions in the preparation method are as follows:

[0141] In S101, water is heated to a temperature of 75°C;

[0142] In S102, the emulsifier, ceramides and oil are mixed and heated to 80°C;

[0143] S103, at a temperature of 75° C., mixing the oil phase and the water phase and homogenizing for 2 min at a homogenizing speed of 1000 rpm to obtain a mixed phase;

[0144] S104, continue stirring the mixed phase at a stirring speed of 15 rpm / min until the temperature reaches 40° C. to obtain an oil-in-water composition.

[0145] Embodiment 35

[0146] Preparation of oil-in-water compositions

[0147] The method is basically the same as Example 1, except that the preparation conditions in the preparation method are as follows:

[0148] In S101, water is heated to a temperature of 80°C;

[0149] In S102, the emulsifier, ceramides and oil are mixed and heated to 85° C.;

[0150] S103, at a temperature of 80° C., mixing the oil phase and the water phase and homogenizing for 3 min at a homogenizing speed of 3000 rpm to obtain a mixed phase;

[0151] S104, continue stirring the mixed phase at a stirring speed of 60 rpm / min until the temperature reaches 35° C. to obtain an oil-in-water composition.

[0152] <Comparative Examples 1-1 to 1-9>

[0153] The oil-in-water composition was prepared according to the preparation method described in S100, which is basically the same as Example 1, except that the formula of the oil-in-water composition is different. The formulas of Comparative Examples 1-1 to 1-9 are shown in Tables 7 to 8.

[0154] Table 7, formula of comparative examples 1-1 to 1-5

[0155]

[0156] Table 8, formula of comparative examples 1-6 to 1-9

[0157]

[0158] <Experiment>

[0159] The compositions of the embodiments and comparative examples are taken as test objects, and the relevant properties are tested through stability test experiments, transepidermal water loss change test experiments, etc., as shown in Experimental Examples 1 to 4.

[0160] 1. Method of stability test experiment:

[0161] Place the object to be tested at the test temperature, store it for a predetermined test time, and observe the crystallization of the object to be tested.

[0162] 2. Method of transepidermal water loss change test experiment:

[0163] The transepidermal water loss change test experiment can reflect the effect of the oil-in-water composition on the moisture change of the skin by measuring the transepidermal water loss change rate over a period of time.

[0164] Trans-epidermal Water Loss (TEWL) refers to the amount of water that evaporates from the skin surface through the stratum corneum. It is usually used to evaluate the skin's barrier function and water retention capacity. It is an important indicator of skin health and can reflect the skin's hydration and the impact of the external environment on the skin. The measurement of trans-epidermal water loss usually uses a special instrument to place sensors on the skin surface to monitor the evaporation of water in real time. The trans-epidermal water loss rate can be further determined based on the trans-epidermal water loss.

[0165] The method of the transepidermal water loss change test experiment includes the following steps:

[0166] Select subjects according to a random table of a predetermined subject list, and use one side of the subject's face as the experimental group; measure the transepidermal water loss and record the data as the transepidermal water loss at the beginning of the test;

[0167] Apply the test object to the face side of the subjects in the experimental group; then place the subjects in a laboratory with a constant temperature and humidity environment at a predetermined test temperature and test humidity for a predetermined test time, and after the test time is reached, measure the transepidermal water loss of each group and record the data as the transepidermal water loss at the test terminal;

[0168] Combining the transepidermal water loss at the beginning of the test and the transepidermal water loss at the end of the test, the transepidermal water loss change rate of the test object is calculated according to the following formula (1);

[0169] Formula (1): Transepidermal water loss change rate = Transepidermal water loss change (△ difference) / Transepidermal water loss at the beginning of the test;

[0170] Among them, the change in transepidermal water loss (△ difference) = the transepidermal water loss at the end of the detection - the transepidermal water loss at the beginning of the detection, that is, the transepidermal water loss at the end of the detection minus the transepidermal water loss at the beginning of the detection is subtracted to get the change in transepidermal water loss (△ difference).

[0171] In addition, the transepidermal water loss can be tested using the Tewameter TM Hex (Courage & Khazaka, Germany).

[0172] <Experimental Example 1>

[0173] 1. Take the compositions prepared in Example 1 and Comparative Examples 1-1 to 1-9 as test objects, respectively, and perform the following tests, and record the results:

[0174] 1. Carry out stability tests at different test temperatures, such as room temperature (25℃±1), low temperature (-15℃±1) and high temperature (45℃±1); specifically, store the test object at the aforementioned different test temperatures for 3.5 months, and observe the crystallization of the test object at two weeks, one month, two months, three months and 3.5 months.

[0175] 2. Conduct transepidermal water loss change tests at different test temperatures, such as room temperature (21℃±1) and low temperature (-8℃±2); the details are as follows:

[0176] 2.1. Normal temperature test:

[0177] At room temperature (21°C ± 1), the transepidermal water loss change rate of each test object was tested after 4 hours and 8 hours of testing time respectively; the test humidity was 40%~60%RH.

[0178] 2.2 Low temperature test:

[0179] At low temperature (-8℃±2℃), the transepidermal water loss change rate of each test object was tested after 1 hour of testing time; the test humidity was 47.1%~86.8%RH.

[0180] 2. Take the composition prepared in Example 1 as the test object, conduct a transdermal moisture control test, and record the results.

[0181] The method for the transdermal moisture control test includes the following steps:

[0182] Subjects were selected according to a pre-determined random list of subjects, with one side of the subject's face as the experimental group and the other side as the control group; facial image capture equipment (MoistureMap MM100) was used to take photos of both sides of the subject's face, and the images were recorded as the moisture distribution images of each group in the initial state;

[0183] Then, the test object is applied to the face side of the subjects in the experimental group, and no product is applied to the face side of the subjects in the control group;

[0184] After using the test object, the subjects were placed in a constant temperature and humidity laboratory under the conditions of low test temperature (-8℃±2℃) and test humidity of 47.1%~86.8%RH for a test time of 1 hour (h). After the test time was reached, a facial image capture instrument (MoistureMap MM100) was used to take pictures of both sides of the subject's face and record them as the moisture distribution images of each group in the post-test state.

[0185] Results and analysis of Experimental Example 1

[0186] 1. Stability test results

[0187] The stability test results of Experimental Example 1 are shown in Table 9 and Figure 1-4 .

[0188] Table 9. Stability test results of the oil-in-water compositions of Example 1 and Comparative Examples 1-1 to 1-9

[0189]

[0190] Note: The meanings of A, B, C and D in the table are as follows:

[0191] A means that the liquid crystal emulsification structure is stable, and there is basically no crystallization of ceramide substances;

[0192] B indicates that the emulsified structure is slightly less stable, and a small amount of ceramide crystals are precipitated;

[0193] C means that there are problems with the stability of the emulsified structure, and a large amount of ceramide crystals are precipitated;

[0194] D indicates that the emulsion structure is unstable, emulsion breaks and a large amount of ceramide substances are precipitated.

[0195] (1) According to the results of Example 1, it can be seen that:

[0196] The oil-in-water composition formed after the oil phase and the water phase are emulsified contains a stable granular liquid crystal emulsified structure distributed in the water. The liquid crystal emulsified structure is stable and can play a good encapsulation role for ceramide substances, thereby preventing ceramides from precipitating in the form of crystals.

[0197] Specifically, at room temperature and low temperature, even after 3.5 months of storage time, the liquid crystal emulsion structure of the water-in-oil composition of Example 1 remained stable, and basically no crystallization of ceramide substances occurred; at high temperature, a small amount of crystallization of ceramide substances did not occur until the third month; this shows that the composition containing sodium di(laurylamide glutamine) lysine and sucrose polystearate in Example 1 has excellent stability and can effectively prevent the crystallization of ceramide substances.

[0198] also, Figure 1 A schematic diagram of the structure of the oil-in-water composition of Example 1 at room temperature (25°C±1) at 3.5 months is recorded, from which it can be seen that the liquid crystal emulsification structure is stable and almost no ceramide crystal precipitation occurs.

[0199] (2) Combining the experimental results of Comparative Examples 1-1 and 1-2, it can be seen that when the emulsifier contains only one of sucrose polystearate and sodium di(lauryl glutamine) lysine, for example, when only sucrose polystearate or sodium di(lauryl glutamine) lysine is contained as the sole emulsifier, there is a problem with the stability of the emulsified structure of the composition. Ceramide substances (cetyl-PG hydroxyethyl palmitamide) do not participate in emulsification at the emulsification interface, but will precipitate in the form of crystals (which may be dispersed in the water phase after precipitation), and therefore cannot form a liquid crystal type oil-in-water composition.

[0200] also, Figure 2 A schematic diagram of the structure of the oil-in-water composition of Comparative Example 1-2 at room temperature (25°C±1) at 3.5 months is recorded, from which it can be seen that the emulsified structure is slightly less stable and a small amount of ceramide substances are crystallized and precipitated. Figure 3 A schematic diagram of the structure of the composition of Comparative Example 1-1 at room temperature (25°C±1) at 3.5 months is recorded, from which it can be seen that there are problems with the stability of the emulsified structure and a large amount of ceramide crystals are precipitated.

[0201] (3) Combined with the experimental results of Comparative Examples 1-3 to Comparative Examples 1-9, it can be seen that when sodium di(lauryl glutamine) lysine is used in combination with other emulsifiers commonly used in the preparation of liquid crystal dosage forms (such as cetearyl alcohol, cetearyl glucoside, hydrogenated lecithin, sodium stearoyl glutamate, sodium methyl stearoyl taurate, sodium stearoyl lactylate, polysorbate 80, etc.), although it is possible to improve the stability of the water-in-oil composition, it cannot effectively prevent crystallization. In addition, when the content of ceramide substances is high (above 0.5%), water and oil are not dissolved, and it is easy to precipitate in the form of crystals at the emulsification interface, affecting the stability of the emulsification interface.

[0202] in addition, Figure 4A schematic diagram of the structure of the oil-in-water composition of Comparative Examples 1-7 at room temperature (25°C±1) at 3.5 months is recorded, from which it can be seen that the emulsified structure is unstable, the emulsion is broken, and a large amount of ceramide substances are precipitated.

[0203] 2. Transepidermal water loss change test results

[0204] The percutaneous test results of Experimental Example 1 are specifically shown in Table 10.

[0205] Table 10 Transepidermal water loss changes of the oil-in-water compositions of Example 1 and Comparative Examples 1-1 to 1-9

[0206]

[0207] It can be seen from Table 10 that, compared with the comparative example, Example 1 has significantly better water loss prevention effect at room temperature and low temperature, and has better moisturizing effect on the skin.

[0208] The oil-in-water composition of Example 1 can effectively reduce transepidermal water loss of the skin under low temperature environment. The possible reasons are: on the one hand, sucrose polystearate and sodium di(laurylamide glutamine) lysine can emulsify ceramide into a liquid crystal structure well; on the other hand, the mixed emulsifier combines well with water and can improve the moisturizing ability.

[0209] In addition, the pH of the skin is weakly acidic, and sodium di(laurylamide glutamine) lysine is an anionic surfactant. After being applied to the skin, under weakly acidic conditions, the HLB value decreases, and it is difficult for the surfactant to exert a good emulsification effect; however, after the emulsified oil-in-water composition of Example 1 is applied to the skin, a closed hydrophobic protective film can be formed on the skin surface, which better prevents the loss of skin moisture and has good product sealing properties.

[0210] 3. Transdermal moisture control test results

[0211] Transdermal moisture control test results are shown in Figures 5 and 6 .

[0212] Figure 5 A schematic diagram showing a comparison of moisture distribution images of the experimental group in the initial state and after the test in the transdermal moisture control test.

[0213] Figure 6 A schematic diagram showing a comparison of the moisture distribution images of the control group in the transdermal moisture control test in the initial state and the state after the test.

[0214] Figures 5 and 6 In the figure, the “BL” on the left corresponds to the moisture distribution image in the initial state, and the “Imm” on the right corresponds to the moisture distribution image in the state after detection.

[0215] according to Figures 5 and 6 The results show that the control group did not use any moisturizing products, so after 1 hour at low temperature (-8℃±2℃), the moisture distribution remained almost unchanged.

[0216] In contrast, since the experimental group used the water-in-oil composition of Example 1, after 1 hour at low temperature (-8°C ± 2°C), the moisture distribution image in the post-test state contained significantly more moisture than that in the initial state, indicating that the water-in-oil composition of Example 1 can enhance the moisturizing ability and better prevent skin moisture loss.

[0217] <Experimental Example 2>

[0218] The oil-in-water compositions prepared in Examples 2 to 27 were taken as test objects, respectively, and the following tests were performed, and the results were recorded:

[0219] 1. Carry out stability tests at different test temperatures, such as room temperature (25℃±1), low temperature (-15℃±1) and high temperature (45℃±1); specifically, store the test object at the aforementioned different test temperatures for 3.5 months, and observe the crystallization of the test object at two weeks, one month, two months, three months and 3.5 months.

[0220] 2. Conduct transepidermal water loss change tests at room temperature (25℃±1) and low temperature (-15℃±1); specifically:

[0221] At room temperature (25℃±1), the transepidermal water loss change rate of each test object was tested after 4 hours and 8 hours respectively;

[0222] At low temperature (-15°C ± 1), the transepidermal water loss change rate of each test object was tested after 1 hour.

[0223] Results and analysis of Experimental Example 2

[0224] 1. Stability test results

[0225] The test results are shown in Tables 11 to 13.

[0226] Table 11. Stability results of the oil-in-water compositions of Examples 2 to 13

[0227]

[0228] Table 12 Stability results of oil-in-water compositions of Examples 14 to 25

[0229]

[0230] Table 13 Stability results of oil-in-water compositions of Examples 26 to 27

[0231]

[0232] Note: The meanings of A, B, C and D in the table are as follows:

[0233] A means that the liquid crystal emulsification structure is stable, and there is basically no crystallization of ceramide substances;

[0234] B indicates that the emulsified structure is slightly less stable, and a small amount of ceramide crystals are precipitated;

[0235] C means that there are problems with the stability of the emulsified structure, and a large amount of ceramide crystals are precipitated;

[0236] D indicates that the emulsion structure is unstable, emulsion breaks and a large amount of ceramide substances are precipitated.

[0237] 2. Transepidermal water loss change test results

[0238] The specific results of the transepidermal water loss change test are shown in Table 14.

[0239] Table 14 Transepidermal water loss changes of oil-in-water compositions of Examples 2 to 27

[0240]

[0241] 3. Results Analysis

[0242] It can be seen from the above results that Examples 2-27 all have the same / similar technical effects as Example 1, and can solve the technical problem of stability of the oil-in-water composition, indicating that the type of ceramide or its analogues is not a limiting factor for the embodiments of the present application to solve the technical problem of stability of the oil-in-water composition and produce the corresponding technical effects, and various types of ceramide or its analogues can be applied to the ceramide-containing oil-in-water composition described in the embodiments of the present application.

[0243] <Experimental Example 3>

[0244] The oil-in-water compositions prepared in Examples 28 to 33 were taken as test objects, respectively, and the following tests were performed, and the results were recorded:

[0245] 1. Carry out stability tests at different test temperatures, such as room temperature (25℃±1), low temperature (-15℃±1) and high temperature (45℃±1); specifically, store the test object at the aforementioned different test temperatures for 3.5 months, and observe the crystallization of the test object at two weeks, one month, two months, three months and 3.5 months.

[0246] 2. Conduct transepidermal water loss change tests at room temperature (25℃±1) and low temperature (-15℃±1); specifically:

[0247] At room temperature (25℃±1), the transepidermal water loss change rate of each test object was tested after 4 hours and 8 hours respectively;

[0248] At low temperature (-15°C ± 1), the transepidermal water loss change rate of each test object was tested after 1 hour.

[0249] Results and analysis of Experimental Example 3

[0250] 1. Stability test results

[0251] The test results are shown in Table 15.

[0252] Table 15. Stability test results of the oil-in-water compositions of Examples 28 to 33

[0253]

[0254] Note: The meanings of A, B, C and D in the table are as follows:

[0255] A means that the liquid crystal emulsification structure is stable, and there is basically no crystallization of ceramide substances;

[0256] B indicates that the emulsified structure is slightly less stable, and a small amount of ceramide crystals are precipitated;

[0257] C means that there are problems with the stability of the emulsified structure, and a large amount of ceramide crystals are precipitated;

[0258] D indicates that the emulsion structure is unstable, emulsion breaks and a large amount of ceramide substances are precipitated.

[0259] 2. Transepidermal water loss change test results

[0260] The specific results of the transepidermal water loss change test are shown in Table 16.

[0261] Table 16 Transepidermal water loss changes of oil-in-water compositions of Examples 28 to 33

[0262]

[0263] 3. Results Analysis

[0264] It can be seen from the above results that Examples 28-33 can achieve the same / similar technical effects as Example 1, and can be used to solve the technical problem of stability of the oil-in-water composition, which means that oil is not a limiting factor for solving the technical problem of stability of the oil-in-water composition and producing corresponding technical effects in the examples of the present application, and various types of oil can be applied to the oil-in-water composition containing ceramides described in the examples of the present application.

[0265] <Experimental Example 4>

[0266] The oil-in-water compositions prepared in Examples 34 to 35 were taken as test objects, respectively, and the following tests were performed, and the results were recorded:

[0267] 1. Carry out stability tests at different test temperatures, such as room temperature (25℃±1), low temperature (-15℃±1) and high temperature (45℃±1); specifically, store the test object at the aforementioned different test temperatures for 3.5 months, and observe the ceramide crystallization precipitation of the test object at two weeks, one month, two months, three months and 3.5 months.

[0268] 2. Conduct transepidermal water loss change tests at room temperature (25℃±1) and low temperature (-15℃±1); specifically:

[0269] At room temperature (25℃±1), the transepidermal water loss change rate of each test object was tested after 4 hours and 8 hours respectively;

[0270] At low temperature (-15°C ± 1), the transepidermal water loss change rate of each test object was tested after 1 hour.

[0271] Results and analysis of Experimental Example 4

[0272] 1. Stability test results

[0273] The test results are shown in Table 17.

[0274] Table 17. Stability test results of the oil-in-water compositions of Examples 34 to 35

[0275]

[0276] Note: The meanings of A, B, C and D in the table are as follows:

[0277] A means that the liquid crystal emulsification structure is stable, and there is basically no crystallization of ceramide substances;

[0278] B indicates that the emulsified structure is slightly less stable, and a small amount of ceramide crystals are precipitated;

[0279] C means that there are problems with the stability of the emulsified structure, and a large amount of ceramide crystals are precipitated;

[0280] D indicates that the emulsion structure is unstable, emulsion breaks and a large amount of ceramide substances are precipitated.

[0281] 2. Transepidermal water loss change test results

[0282] The specific results of the transepidermal water loss change test are shown in Table 18.

[0283] Table 18 Transepidermal water loss changes of the oil-in-water compositions of Examples 34 to 35

[0284]

[0285] 3. Results Analysis

[0286] It can be seen from the above results that Examples 34 to 35 can achieve the same / similar technical effects as Example 1, and all contain a relatively stable granular liquid crystal emulsion structure distributed in water. The liquid crystal emulsion structure is stable and can play a good encapsulation role for ceramides, which can reduce or prevent the precipitation of ceramide substances in the form of crystals, and can be used to solve the technical problem of the stability of water-in-oil compositions.

[0287] The above results indicate that, in the method for preparing the oil-in-water composition provided in the examples of the present application, the preparation conditions such as the heating temperature for obtaining the water phase (75-85°C), the heating temperature for obtaining the oil phase (80-95°C), the temperature for obtaining the mixed phase (75-85°C), the homogenization time (2-5 min), the homogenization speed (1000-3000 rpm), and the stirring speed (15-60 rpm / min) are not limiting factors for solving the technical problem of the stability of the oil-in-water composition and producing the corresponding technical effects in the examples of the present application.

[0288] The above specific embodiments have been described in detail for the present invention, but these do not constitute limitations on the present invention. The protection scope of the present invention is not limited to the above embodiments, and any equivalent modifications or changes made by ordinary technicians in the field based on the content disclosed by the present invention should be included in the protection scope recorded in the claims.

Claims

1. An oil-in-water composition containing ceramides, characterized in that: Ceramides, wherein the ceramides are selected from ceramides or ceramide analogs, wherein the ceramide analogs are selected from one or more of cetyl-PG hydroxyethyl palmitamide, hydroxyethyl palmitoxy hydroxypropyl palmitamide, dihydrosphingosine, hexanoylsphingosine, hydroxypalmitoyl dihydrosphingosine, salicylphytosphingosine, tetraacetylphytosphingosine, acetylphytosphingosine, and phytosphingosine, and the content of the ceramides is 1-3%; Oil, wherein the oil is selected from one or more of neopentyl glycol dicaprylate / dicaprate, pentaerythritol tetra(ethylhexanoate), olive oil, sunflower seed oil, squalane, mineral oil, dibutyl adipate, coconut oil alcohol-caprylate / caprate; An emulsifier comprising sucrose polystearate and sodium di(laurylamide glutamine) lysine, wherein the mass ratio of the sucrose polystearate to the sodium di(laurylamide glutamine) lysine is 5-60:1, the content of the emulsifier is 0.6-6.1%, the content of the sucrose polystearate is 0.5-6%, and the content of the sodium di(laurylamide glutamine) lysine is 0.01-0.3%; and water; among them, The ceramide-containing oil-in-water composition is emulsified based on the ceramides, the emulsifier, the oil, and the water.

2. The oil-in-water composition containing ceramides according to claim 1, characterized in that The content of the sucrose polystearate is 2-6%.

3. The oil-in-water composition containing ceramides according to claim 1, characterized in that: The content of the sucrose polystearate is 0.5-2%.

4. The oil-in-water composition containing ceramides according to claim 1, characterized in that: The ceramide is selected from one or more of ceramide EOP, ceramide AS, ceramide AP, ceramide NS / ceramide NG, and ceramide NP.

5. The oil-in-water composition containing ceramides according to claim 1, characterized in that: The mass content of the oil is 0.5-30%.

6. The oil-in-water composition containing ceramides according to claim 1, characterized in that: The content of sodium di(laurylamide glutamine) lysine is 0.1%; the content of the oil is 10-15%.

7. The oil-in-water composition containing ceramides according to claim 1, characterized in that: It also includes one or more of oil-soluble components and water-soluble components.

8. A method for preparing an oil-in-water composition containing ceramides, characterized in that: include: heating water to a temperature of 75 to 85° C. to obtain an aqueous phase; The emulsifier, ceramides and oil are mixed and heated to 80-95° C., and the oil phase is obtained after stirring; wherein the ceramide is selected from ceramide or ceramide analogs, the ceramide analogs are selected from one or more of cetyl-PG hydroxyethyl palmitamide, hydroxyethyl palmitoxy hydroxypropyl palmitamide, dihydro(ceramide)sphingosine, caproyl ceramide, hydroxypalmitoyl dihydrosphingosine, salicyloyl phytosphingosine, tetraacetyl phytosphingosine, acetyl phytosphingosine, and phytosphingosine, the oil is selected from one or more of neopentyl glycol dicaprylate / dicaprate, pentaerythritol tetra(ethylhexanoate), olive oil, sunflower seed oil, squalane, mineral oil, dibutyl adipate, coconut oil-caprylate / caprate, the emulsifier includes sucrose polystearate and sodium di(lauroyl glutamine) lysine, the content of the ceramide is 1-3%, and the content of the emulsifier is 0. 6-6.1%, the mass ratio of the sucrose polystearate to the di(laurylamide glutamine) sodium lysine is 5-60:1, the content of the sucrose polystearate is 0.5-6%, and the content of the di(laurylamide glutamine) sodium lysine is 0.01-0.3%; At a temperature of 75 to 85° C., the oil phase and the water phase are mixed and homogenized for 2 to 5 minutes at a homogenization speed of 1000 to 3000 rpm to obtain a mixed phase; The mixed phase is stirred continuously at a stirring speed of 15 to 60 rpm / min until the temperature is no higher than 45° C. to obtain an oil-in-water composition containing amides.

9. The method for preparing the oil-in-water composition containing ceramides according to claim 8, characterized in that: The ceramide is selected from one or more of ceramide EOP, ceramide AS, ceramide AP, ceramide NS / ceramide NG, and ceramide NP; The content of sucrose polystearate is 0.5-2% or 2-6%; The content of sodium di(laurylamide glutamine) lysine is 0.1%; The oil content is 0.5-30%; The water content is the balance.

10. The method for preparing the oil-in-water composition containing ceramides according to claim 9, characterized in that: The oil content is 10-15%; The step of heating water to a temperature of 75 to 85° C. to obtain the aqueous phase comprises: heating water and water-soluble components to a temperature of 75 to 85° C.; The method comprises: mixing the emulsifier, ceramides and oil, heating the mixture to 80-95° C., and stirring the mixture to obtain the oil phase; and comprising: mixing the emulsifier, ceramides, oil and oil-soluble components, heating the mixture to 80-95° C., and stirring the mixture to obtain the oil phase.

11. An oil-in-water composition containing ceramides prepared according to the method for preparing an oil-in-water composition containing ceramides according to any one of claims 8 to 10.

12. Use of the ceramide-containing oil-in-water composition according to any one of claims 1 to 7 and 11 in the preparation of skin care products.

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