Cell culture device for regenerative medicine

Abstract

The invention discloses a regenerative medicine cell culture device which comprises a culture box, partition plates, heat conduction particles, a culture bottle and a temperature controller, the multiple layers of partition plates are arranged in the culture box, the partition plates are provided with grooves, the multiple heat conduction particles are arranged in the grooves, the heat conduction particles are used for covering the periphery of the culture bottle, and the temperature controller is arranged on the periphery of the culture bottle. The temperature controller is arranged on the partition plate, an air outlet is formed in the bottom of the partition plate, the air outlet is communicated with the air inlet end of a first pump body through a first pipeline, and the air outlet end of the first pump body is communicated into the incubator. The invention provides a regenerative medicine cell culture device which can accurately control the temperature of cell culture.

Description

Technical Field

[0001] The invention relates to the technical field of cell culture, in particular to a cell culture device for regenerative medicine. Background Art

[0002] With the rapid development of stem cell technology, gene editing technology, and tissue engineering technology, cell culture in regenerative medicine has become a key means in the fields of disease treatment, tissue repair, and organ regeneration. In the cell culture process of regenerative medicine, in addition to specific culture media, growth factors, cytokines and other key elements, precise control of the culture environment is also crucial. This includes but is not limited to strict regulation of parameters such as temperature, humidity, and oxygen concentration to ensure that cells can grow and differentiate normally under optimal conditions.

[0003] However, the existing temperature control methods still have many shortcomings in terms of cell culture temperature control. The direct heating method usually heats the bottom of the cell culture bottle, and then conducts heat through the inside of the culture bottle. This method often leads to uneven temperature distribution inside the culture bottle, affecting cell growth. Although the air heating method can directly conduct heat to the surroundings of the culture bottle, the air is easily blocked during the flow, especially when multiple culture bottles are placed in the incubator. The temperature difference in different areas will be more obvious, and it is difficult to achieve accurate control of the temperature of the culture bottle. Therefore, the existing temperature control method has certain limitations in cell culture in regenerative medicine, and cannot meet the needs of high-precision control of culture temperature. Summary of the invention

[0004] In view of the above-mentioned prior art, the present invention provides a cell culture device for regenerative medicine, which can more accurately control the temperature of cell culture.

[0005] To achieve the above object, the technical solution of the embodiment of the present invention is implemented as follows:

[0006] A cell culture device for regenerative medicine comprises an incubator, a partition, heat-conducting particles, a culture bottle and a temperature controller. The incubator is provided with multiple layers of the partition, the partition is provided with a groove, a plurality of the heat-conducting particles are provided in the groove, the heat-conducting particles are used to cover the outer circumference of the culture bottle, the partition is provided with the temperature controller, the bottom of the partition is provided with an air outlet, the air outlet is connected to the air inlet end of a first pump body through a first pipe, and the air outlet end of the first pump body is connected to the incubator.

[0007] Furthermore, the temperature controller includes a heating wire, a controller and a temperature sensor. The temperature sensor is arranged in the groove, and the thermal conductive particles cover the temperature sensor. The heating wire is located at the bottom of the partition. The heating wire and the temperature sensor are connected to the controller signal.

[0008] Furthermore, the partition is provided with a through hole, and the through hole is provided with an air duct, the air duct connects the upper and lower sides of the partition, and the top of the air duct is flush with the top of the partition.

[0009] Furthermore, a light guide tube is provided in the groove, the light guide tube is provided with a light outlet, the light guide tube is connected to a light shield, and an ultraviolet lamp is provided in the light shield.

[0010] Furthermore, a shielding cover is provided above the partition, and a reflective layer is provided below the shielding cover.

[0011] Furthermore, a second air pump is provided in the incubator, an air inlet of the second air pump is connected to the outside, an air outlet of the second air pump is connected to a filter and an air sterilizing device in sequence, and the sterilizing device is connected to the inner cavity of the incubator.

[0012] Furthermore, a horizontal plate is provided at the bottom of the partition, and a plurality of first through holes are provided on the horizontal plate. The inner diameter of the first through holes is smaller than the diameter of the thermally conductive particles, and the air outlet is located below the horizontal plate.

[0013] Furthermore, the horizontal plate is made of a transparent material, a reflective coating is provided on the bottom of the partition, and an ultraviolet lamp is provided below the horizontal plate.

[0014] Furthermore, the side surface of one side of the first through hole is perpendicular to the top surface of the horizontal plate, and the side surface of the other side is smoothly transitioned to the top surface of the horizontal plate. The horizontal plate is slidably connected to the partition, and the partition is provided with a first spring connected to the horizontal plate. The partition is provided with a guide block, and the guide block is elastic. The outlet is facing the guide block.

[0015] Furthermore, a vertical plate is provided on one side of the partition, and the vertical plate is slidably connected to the partition. The partition is provided with a second spring connected to the vertical plate, and the vertical plate is provided with a second through hole. The side surface of the second through hole facing one side is perpendicular to the surface of the vertical plate, and the side surface of the second through hole facing the upper side smoothly transitions to the surface of the vertical plate. The vertical plate is rotatably connected to one end of the connecting rod, and the horizontal plate is rotatably connected to the other end of the connecting rod.

[0016] The beneficial effects of the present invention are as follows: heat is conducted by covering the periphery of the culture bottle with heat-conductive particles, and the heat-conductive particles will not evaporate, thereby maintaining the environmental humidity in the incubator, which is beneficial to the culture of cells. The heat-conductive particles in the partition are heated by a thermostat, and the heat is conducted to the culture bottle through the heat-conductive particles, thereby achieving effective control of the temperature of the culture bottle and promoting the growth and differentiation of cells. When the first pump body is working, it drives the air to be discharged from the air outlet, promotes heat exchange between the heat-conductive particles, and makes the temperature of the heat-conductive particles in the groove uniform. At the same time, the air circulates in the incubator, avoiding differences in air quality in different areas, reducing dead corners of air flow in the incubator, and ensuring the stability of the environment in the incubator. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic diagram of the structure of a cell culture device for regenerative medicine in Example 1 of the present application;

[0018] Figure 2 This is a schematic diagram of the structure of a cell culture device for regenerative medicine in Example 2 of the present application;

[0019] Figure 3 This is a schematic diagram of the connection structure of 2 horizontal plates and vertical plates in an embodiment of the present application;

[0020] Description of Figure Numbers:

[0021] 1. Incubator; 2. Partition; 3. Heat-conducting particles; 4. Culture bottle; 5. Heating wire; 6. Temperature sensor; 7. Air outlet; 8. First pipeline; 9. First pump body; 10. Air guide tube; 11. Light guide tube; 12. Light outlet; 13. Light cover; 14. Ultraviolet lamp; 15. Shielding cover; 16. Second air pump; 17. Filter; 18. Air sterilization device; 19. Horizontal plate; 20. First through hole; 21. First spring; 22. Guide block; 23. Vertical plate; 24. Second spring; 25. Second through hole; 26. Connecting rod; 27. Groove. DETAILED DESCRIPTION

[0022] The technical solution of the present invention is further described in detail below in conjunction with the drawings and specific embodiments of the specification. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used in the specification of the present invention herein are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

[0023] It should also be noted that when an element is referred to as being "fixed to" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "inside", "outside", "left", "right" and similar expressions used herein are for illustrative purposes only and are not intended to be the only implementation method.

[0024] Example 1

[0025] Please refer to the attached Figure 1 The present application provides a cell culture device for regenerative medicine, comprising a culture box 1, a partition 2, heat-conducting particles 3, a culture bottle 4 and a temperature controller. The culture box 1 is provided with multiple layers of the partition 2, the partition 2 is provided with a groove 27, and a plurality of the heat-conducting particles 3 are provided in the groove 27. The heat-conducting particles 3 are used to cover the outer periphery of the culture bottle 4. The partition 2 is provided with the temperature controller. The bottom of the partition 2 is provided with an air outlet 7, and the air outlet 7 is connected to the air inlet end of the first pump body 9 through a first pipe 8. The air outlet end of the first pump body 9 is connected to the culture box 1. During the culture process, the cells are placed in the culture bottle 4, and then the culture bottle 4 is placed in the groove 27 of the partition 2. The culture bottle 4 is covered with the heat-conducting particles 3. The heat-conducting particles 3 have good thermal conductivity and can conduct heat to the culture bottle 4. The heat-conducting particles 3 will not evaporate during heat conduction, so the environmental humidity in the culture box 1 can be maintained, and the humidity of the culture environment can be well controlled, which is convenient for cell culture. During cell culture, the metal particles in the partition 2 are heated by a thermostat, and the metal particles are used to conduct heat to the culture bottle 4, which can effectively control the temperature of the culture bottle 4 and promote cell growth and differentiation. When the first pump body 9 is working, it drives air to be discharged from the air outlet 7, and the air is discharged from the top after passing through the gaps of the heat-conducting particles 3. The airflow promotes heat exchange between the heat-conducting particles 3, so that the temperature of the heat-conducting particles 3 in the groove 27 is uniform. At the same time, under the action of the air pump, the air circulates in the incubator 1, which can promote the air temperature contacted by the culture bottle 4, avoid different air quality in different areas, reduce the dead corners of air flow in the incubator 1, and ensure that the environment in the incubator 1 is maintained in a stable range.

[0026] Specifically, the temperature controller includes a heating wire 5, a controller and a temperature sensor 6. The temperature sensor is arranged in the groove 27, and the heat-conducting particles 3 cover the temperature sensor 6. The heating wire 5 is located at the bottom of the partition 2. The heating wire 5 and the temperature sensor 6 are connected to the controller signal. The heating wire 5 is controlled by the controller to heat, and the temperature in the groove 27 is monitored in real time by the temperature sensor 6, so as to accurately control the temperature of the culture bottle 4, which is conducive to the growth and differentiation of cells.

[0027] Specifically, the partition 2 is provided with a through hole, and the through hole is provided with an air duct 10, the air duct 10 connects the upper and lower sides of the partition 2, and the top of the air duct 10 is flush with the top of the partition 2. The air duct 10 connects the upper and lower sides of the partition 2, allowing the upper and lower air of the partition 2 to circulate directly. When the incubator 1 is turned on, when the partition 2 is raised upward or lowered downward, the air above and below the partition 2 can circulate with each other, which can prevent the outside air from entering the incubator 1 in large quantities due to the up and down movement of the partition 2, and can reduce the possibility of the incubator 1 environment being polluted. The top of the air duct 10 is parallel to the partition 2, which can prevent the thermal conductive particles 3 in the groove 27 from falling into the air duct 10.

[0028] Optionally, a light guide 11 is provided in the groove 27, the light guide 11 is provided with a light outlet 12, the light guide 11 is connected to a light shield 13, and an ultraviolet lamp 14 is provided in the light shield 13. When the ultraviolet lamp 14 is working, ultraviolet light is emitted, the light propagates along the light guide 11, and then is emitted from the light outlet 12 to irradiate the thermally conductive particles 3. Under the irradiation of ultraviolet light, the bacteria on the thermally conductive particles 3 can be inactivated, thereby reducing the contamination in the incubator 1.

[0029] Specifically, a shielding cover 15 is provided above the partition 2, and a reflective layer is provided below the shielding cover 15. After the light overflows the surface of the thermally conductive particles 3, it is reflected back again through the shielding cover 15, thereby improving the sterilization effect of the ultraviolet lamp 14. Preferably, the surface of the thermally conductive particles 3 has a reflective layer, which can reflect ultraviolet rays and improve the sterilization effect of ultraviolet rays.

[0030] Specifically, the incubator 1 is provided with a second air pump 16, the air inlet end of the second air pump 16 is connected to the outside, the air outlet end of the second air pump 16 is connected to a filter 17 and an air sterilizing device 18 in sequence, and the sterilizing device is connected to the inner cavity of the incubator 1. The outside air is pumped into the incubator 1 by the second air pump 16. After the door of the incubator 1 is opened, the air is continuously pumped into the incubator 1, so that the air pressure in the incubator 1 is slightly higher than the atmospheric pressure, which prevents the outside air from entering the incubator 1 through the opening, and prevents the incubator 1 from being contaminated. The air pumped away by the second air pump 16 is filtered by the filter 17, and then sterilized by the air sterilizing device 18 before entering the incubator 1, ensuring that the air entering the incubator 1 is clean.

[0031] Example 2

[0032] Please refer to the attached Figures 2-3The difference between this embodiment and embodiment 1 is that a horizontal plate 19 is provided at the bottom of the partition plate 2, and the horizontal plate 19 is provided with a plurality of first through holes 20, the inner diameter of the first through holes 20 is smaller than the diameter of the thermally conductive particles 3, and the gas outlet 7 is located below the horizontal plate 19. The thermally conductive particles 3 cannot pass through the first through holes 20, and the gas entering the bottom of the horizontal plate 19 from the gas outlet 7 needs to pass through the first through holes 20 of the horizontal plate 19 when flowing upward. The horizontal plate 19 is used to promote the uniform passage of the airflow at the bottom, which can improve the uniformity of the airflow and reduce the airflow difference in different areas.

[0033] Specifically, the horizontal plate 19 is made of a transparent material, a reflective coating is provided on the bottom of the partition 2, and an ultraviolet lamp 14 is provided below the horizontal plate 19. The transparent horizontal plate 19 allows light to pass directly, and the ultraviolet lamp 14 emits ultraviolet light to sterilize the thermal conductive particles 3, thereby eliminating bacteria on the thermal conductive particles 3 and maintaining a stable environment in the incubator 1.

[0034] Specifically, the side surface of one side of the first through hole 20 is perpendicular to the top surface of the horizontal plate 19, and the side surface of the other side is smoothly transitioned to the top surface of the horizontal plate 19. The horizontal plate 19 is slidably connected to the partition 2. The partition 2 is provided with a first spring 21 connected to the horizontal plate 19. The partition 2 is provided with a guide block 22, and the guide block 22 is elastic. The outlet is directly opposite to the guide block 22. When the airflow blows to the guide block 22, the guide block 22 vibrates under the action of the airflow, and this vibration is transmitted to the horizontal plate 19, thereby causing the vibration of the horizontal plate 19. The reciprocating vibration of the horizontal plate 19 in the horizontal direction will generate thrust on the thermal conductive particles 3. Since the friction forces on the thermal conductive particles 3 on both sides of the first through hole 20 are different, the thermal conductive particles 3 have a situation of moving to the other side during the reciprocating vibration. The thermal conductive particles 3 located at the bottom will change during the continuous vibration, so that different thermal conductive particles 3 can be exposed to ultraviolet radiation. Meanwhile, during the movement of the thermally conductive particles 3 , different surfaces of the thermally conductive particles 3 will also face the ultraviolet lamp 14 , so that the thermally conductive particles 3 are more evenly exposed to the ultraviolet rays.

[0035] Specifically, a vertical plate 23 is provided on one side of the partition 2, and the vertical plate 23 is slidably connected to the partition 2. The partition 2 is provided with a second spring 24 connected to the vertical plate 23. The vertical plate 23 is provided with a second through hole 25, and the side of the second through hole 25 facing one side is perpendicular to the surface of the vertical plate 23, and the side of the second through hole 25 facing the upper side is smoothly transitioned to the surface of the vertical plate 23. The vertical plate 23 is rotatably connected to one end of the connecting rod 26, and the horizontal plate 19 is rotatably connected to the other end of the connecting rod 26. When the horizontal plate 19 vibrates, the vertical plate 23 will be driven to move through the connecting rod 26, so that the vertical plate 23 vibrates in the vertical direction. The reciprocating vibration of the vertical plate 23 in the vertical direction will generate thrust on the thermal conductive particles 3. Since the friction forces on the thermal conductive particles 3 on both sides of the second through hole 25 are different, the thermal conductive particles 3 have a situation of moving upward in the reciprocating vibration. In the continuous vibration, the thermal conductive particles 3 located on the side will change, and the thermal conductive particles 3 on the side will move upward. The horizontal plate 19 pushes the thermally conductive particles 3 at the bottom to the side close to the vertical plate 23, and the vertical plate 23 pushes the thermally conductive particles 3 at the bottom upward, thereby forming an effective flow of the thermally conductive particles 3, allowing the thermally conductive particles 3 to contact with ultraviolet rays for sterilization.

[0036] The above are only specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art can easily think of changes or substitutions within the technical scope disclosed by the present invention, which should be included in the protection scope of the present invention. The protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. A cell culture apparatus for regenerative medicine, characterized in that: It includes an incubator, a partition, heat-conductive particles, a culture bottle and a temperature controller. The incubator is provided with multiple layers of the partition, the partition is provided with a groove, a number of the heat-conductive particles are provided in the groove, the heat-conductive particles are used to cover the outer circumference of the culture bottle, the partition is provided with the temperature controller, and the bottom of the partition is provided with an air outlet, the air outlet is connected to the air inlet end of the first pump body through a first pipe, and the air outlet end of the first pump body is connected to the incubator.

2. A cell culture device for regenerative medicine according to claim 1, characterized in that: The temperature controller includes a heating wire, a controller and a temperature sensor. The temperature sensor is arranged in the groove, and the thermal conductive particles cover the temperature sensor. The heating wire is located at the bottom of the partition. The heating wire and the temperature sensor are connected to the controller signal.

3. A cell culture device for regenerative medicine according to claim 1, characterized in that: The partition is provided with a through hole, and the through hole is provided with an air guide pipe, the air guide pipe is connected with the upper and lower sides of the partition, and the top of the air guide pipe is flush with the top of the partition.

4. The cell culture device for regenerative medicine according to claim 1, characterized in that: A light guide tube is arranged in the groove, the light guide tube is provided with a light outlet, the light guide tube is connected with a light cover, and an ultraviolet lamp is arranged in the light cover.

5. The cell culture device for regenerative medicine according to claim 4, characterized in that: A shielding cover is provided above the partition, and a reflective layer is provided below the shielding cover.

6. The cell culture device for regenerative medicine according to claim 1, characterized in that: A second air pump is provided in the incubator, an air inlet of the second air pump is connected to the outside, an air outlet of the second air pump is connected to a filter and an air sterilizing device in sequence, and the sterilizing device is connected to the inner cavity of the incubator.

7. The cell culture apparatus for regenerative medicine according to claim 1, characterized in that: A horizontal plate is provided at the bottom of the partition, and a plurality of first through holes are provided on the horizontal plate. The inner diameter of the first through holes is smaller than the diameter of the heat-conducting particles, and the air outlet is located below the horizontal plate.

8. The cell culture device for regenerative medicine according to claim 7, characterized in that: The horizontal plate is made of transparent material, a reflective coating is provided on the bottom of the partition, and an ultraviolet lamp is provided below the horizontal plate.

9. The cell culture device for regenerative medicine according to claim 7, characterized in that: The side surface of one side of the first through hole is perpendicular to the top surface of the horizontal plate, and the side surface of the other side is smoothly transitioned to the top surface of the horizontal plate. The horizontal plate is slidably connected to the partition, and the partition is provided with a first spring connected to the horizontal plate. The partition is provided with a guide block, and the guide block is elastic. The outlet is facing the guide block.

10. The cell culture device for regenerative medicine according to claim 9, characterized in that: A vertical plate is provided on one side of the partition, and the vertical plate is slidably connected to the partition. The partition is provided with a second spring connected to the vertical plate. The vertical plate is provided with a second through hole. The side surface of the second through hole facing one side is perpendicular to the surface of the vertical plate, and the side surface of the second through hole facing the upper side smoothly transitions to the surface of the vertical plate. The vertical plate is rotatably connected to one end of the connecting rod, and the horizontal plate is rotatably connected to the other end of the connecting rod.

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