Pathological slide dyeing device based on advanced fluid control technology

Abstract

The invention relates to the field of automation of pathological slide staining, in particular to an automation device for cytological slide staining and histological immunohistochemistry and special staining based on an advanced fluid control technology, which comprises an upper centrifugal component and a lower slide staining component, and the slide-making and dyeing assembly comprises a circular or arc-shaped dyeing turntable and a matched traditional dyeing or immunohistochemical and special dyeing liquid path component. According to the invention, a traditional transfer mode is not adopted, such as a pipette arm, a pipette and other structures which are easy to generate mechanical faults, key function modules such as sample oscillation, centrifugation, flaking, dyeing and the like are integrated in one machine, cytology sample pretreatment and flaking dyeing operations are fully automatically completed, and special dyeing functions such as histology immunohistochemistry and the like can be compatible; tedious steps are greatly simplified, the functional diversity, efficiency and stability of the equipment are improved, and the maintenance cost of the equipment is also reduced.

Description

Technical Field

[0001] The invention relates to the field of automated pathological slide preparation and staining, and in particular to an automated device for cytological slide preparation and staining, histological immunohistochemistry and special staining based on advanced fluid control technology. Background Art

[0002] Clinical pathology can be divided into histopathology and cytopathology. The most commonly used sample processing method for histology is paraffin sectioning; centrifugal sedimentation preparation and single drop staining are ideal sample pretreatment methods for cytology; histopathology and cytopathology staining methods include traditional chemical staining, such as HE staining, Papanicolaou staining, etc.; and special staining, such as immunohistochemistry and special staining. However, the traditional manual and various semi-automatic preparation and staining processes developed later are cumbersome and inefficient. Most of the semi-automatic equipment on the market can only realize a single histology, cytology or tissue chip preparation or a certain staining function, but cannot complete multiple functions at the same time, as well as the automation of the entire process. Among them, the traditional sedimentation liquid-based cell preparation and staining semi-automatic process usually involves multiple independent steps, including sample shaking, transfer, centrifugation and preparation and staining, and requires manual multiple sample transfers, transfers between equipment and labeling operations. The workflow is not only complicated and time-consuming, but also prone to human errors, leading to errors in experimental results.

[0003] In addition, the traditional method also requires multiple instruments, such as oscillating transfer machines, centrifuges, slide preparation and staining machines, immunohistochemical staining machines, etc., which not only increases the equipment cost, but also takes up a lot of laboratory space, and the equipment failure rate is high. Therefore, the market is in urgent need of a device and method that can integrate and automate the entire process of cytology and histology slide preparation and staining. Large laboratories also need high-throughput production lines to improve experimental efficiency, reduce operational complexity and costs, reduce equipment space, and reduce failure rates.

[0004] Traditional fluid control methods are used in in vitro diagnostic applications, such as transferring sample liquid from one container to another, usually with the help of an intermediate carrier, such as a pipette gun and a disposable pipette tip. However, due to the need for repeated high-frequency operations and the workmanship of disposable pipette tips, it is easy to cause empty sample aspiration, missed aspiration, inaccurate quantification, etc., resulting in erroneous experimental results.

[0005] The traditional fluid control method commonly used in pathological slide preparation and staining uses a three-axis robotic arm to move the gun head, needle, and pipeline. Due to the multi-directional mechanical movement, the positioning is inaccurate. To prevent contamination, it is necessary to clean the parts or replace disposable consumables such as the pipette tip after each use; a set of mechanical systems can only complete one liquid transfer at a time, or complete a functional action, such as centrifugation, oscillation, or pipetting, etc.; if the oscillation function module is installed in the integrated instrument, the ultrasonic vibration motor is usually sampled, resulting in high instrument costs, cell samples are easily deformed, affecting diagnosis, and other problems, therefore, the efficiency is low; the path is too long, and the pipeline has too many bends, resulting in leakage of liquid and air, and errors in adding liquid and aspirating liquid are prone to occur. Therefore, traditional fluid-controlled instruments require more frequent maintenance, calibration, and other work to ensure that the instrument works stably and reliably. However, advanced fluid control methods and technologies can solve the above problems well. Summary of the invention

[0006] The purpose of the present invention is to provide a pathology slide preparation and staining device based on advanced fluid control technology to solve the problems of the traditional manual and various semi-automatic slide preparation and staining equipment developed later proposed in the above background technology, such as being cumbersome, unstable, requiring multiple devices, having high manufacturing and use costs and low efficiency.

[0007] To achieve the above-mentioned purpose, the present invention provides an automated device for pathological slide preparation and staining based on advanced fluid control technology, characterized in that: one machine integrates and fully automatically completes the centrifugal sedimentation cytology sample pre-treatment and slide preparation and staining functions, and can be optionally installed on the same device and replace some consumables during use, switching to cytology, histology, tissue chips, high-throughput assembly line forms, and adapting to special staining such as immunohistochemistry or other needs. Its structure includes an upper centrifugal assembly and a lower slide preparation and staining assembly, the centrifugal assembly includes a centrifugal drive component and an upper centrifugal liquid path component, the slide preparation and staining assembly includes an arc-shaped staining turntable component and an upper staining liquid path component; a plurality of slide preparation and staining units and a plurality of waste liquid tanks are arranged on the staining turntable; the liquid after the centrifugal assembly completes the centrifugation is directly dumped and transferred to the slide preparation and staining assembly or the waste liquid tank below.

[0008] As a preferred solution of the present invention, a plurality of centrifugal units are annularly installed on the outer side of the centrifugal drive component, and the centrifugal units include hanging baskets, and a plurality of centrifugal test tubes are arranged inside the hanging baskets.

[0009] As a preferred embodiment of the present invention, the upper centrifugal assembly and the lower slide-making and staining assembly can be controlled by an external motor to perform relative up and down movement and rotational positioning, and a lifting plate is installed on the staining turntable, and the lifting plate is located below the centrifugal unit; the lifting plate lifts the centrifugal unit so that the positions of the slide-making and staining unit or the waste liquid tank and the centrifugal unit correspond one to one through positioning, and the centrifugal unit is flipped to directly pour the liquid in the centrifugal test tube into the slide-making and staining unit or the waste liquid tank.

[0010] As a preferred embodiment of the present invention, the centrifugal test tube can also be used as a cell sample preservation liquid bottle; a sleeve is added close to the inner wall of the centrifugal test tube, the outlet at the lower end of the sleeve is close to the bottom of the centrifugal test tube, and a filter is installed, the filter is close to the bottom of the centrifugal test tube, after sampling, a sampling brush or a substitute stirring object is placed on the outside of the sleeve, and the tip of the sampling brush or the substitute stirring object is facing the bottom of the centrifugal test tube; a vibration motor (328) is installed below the centrifugal test tube (32), and the two cooperate to oscillate more fully to disperse the cell sample.

[0011] As a preferred embodiment of the present invention, the centrifugal fluid path component includes a centrifugal test tube liquid adding needle, a driving pump and matching pipelines and reagent bottles; the centrifugal test tube liquid adding needle is fixed above the centrifugal unit, and the centrifugal driving component is rotated by a centrifugal positioning motor to position the corresponding liquid adding position.

[0012] As a preferred embodiment of the present invention, the liquid path components of the slide preparation and staining assembly are divided into two types: one is a liquid path component used for traditional cytological staining, such as Papanicolaou staining and HE staining; the other is a liquid path component specifically used for immunohistochemistry and special staining (including tissue chips) of cytology and histology.

[0013] As a preferred embodiment of the present invention, the conventional staining liquid path component comprises a plurality of liquid adding needles, a plurality of waste liquid suction needles, matching pipelines and a driving pump and a reagent bottle / liquid storage bottle, a glass slide and a conventional staining special slide making chamber.

[0014] As a preferred embodiment of the present invention, a liquid circuit component specifically used for immunohistochemistry and special staining includes a number of special antibody reagent bottles and precise liquid adding needles, a number of single-head or multi-head immunohistochemistry and special staining aspiration needles, supporting pipelines and drive pumps and reagent bottles / liquid storage bottles, special staining special slides, and a slide preparation chamber-dispenser.

[0015] As a preferred embodiment of the present invention, the special staining glass slide is a glass slide with a hydrophobic coating separating several slide adhesion areas. The slide making chamber-dispenser is placed on the upper part of the special staining glass slide, and the upper layer is provided with a liquid storage tank, an overflow port and several flow channels. The thickness and length of the flow channels are designed according to the flow rate. The end of the flow channel has a downward channel, and the liquid can be dripped onto the slide making hole of the glass slide; the excess liquid is discharged through the overflow slot to ensure the consistency of the amount of liquid added each time. In addition, the liquid pipette is aimed at the channel for suction, and the waste liquid on the corresponding slide making hole and the slide making chamber-dispenser can be sucked away; the lower end of the dripping channel is close to the slide making area, and it plays the function of multi-porous equal distribution of liquid when dripping liquid. The bottom of the slide making chamber-dispenser is provided with several steam holes, and a water bath is provided below the slide making chamber-dispenser. The adjustable heating liquid generates steam, and the steam can enter the top of the glass slide through the steam hole at the bottom of the slide making chamber-dispenser, which plays the role of keeping the sample warm and moisturizing.

[0016] As a preferred embodiment of the present invention, a piston is provided on the reagent bottle of the immunohistochemistry and special staining dedicated liquid circuit system, which also serves as an injection pump; the reagent bottle is equipped with a single-head or multi-head precision liquid addition needle, and the immunohistochemistry and special staining dedicated liquid circuit system adopts a multi-head immunohistochemistry and special staining liquid aspiration needle.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] 1. The pathological slide preparation and staining device based on advanced fluid control technology realizes a high degree of integration and automation of the slide preparation and staining process. By integrating key components such as centrifugal components, turntables, centrifugal units, slide preparation and staining plates, and pipetting components, the present invention can complete the entire process from sample preparation, centrifugal treatment to slide preparation and staining on the same device, greatly simplifying the cumbersome steps of the traditional slide preparation and staining process. This not only significantly improves experimental efficiency and reduces operational complexity, but also reduces equipment costs and laboratory space occupancy, and reduces equipment failure rates. This device can be optionally equipped with different components and consumables to present itself in the form of cytology, histological staining, immunohistochemistry and special staining, tissue chip staining, and high-throughput assembly lines to meet different slide preparation and staining functions and improve the diversity of equipment functions.

[0019] 2. In this pathology preparation and staining device based on advanced fluid control technology, the centrifugal test tube is also used as a bottle for cell sample preservation fluid; the gradient density centrifugation and coarse impurity filtration steps are carried out simultaneously; and before centrifugation, the cell sample does not need to be pre-shaken and can be directly put into the machine; after centrifugation, the sample is broken up by a mechanical vibration motor, which is cheaper than an ultrasonic motor and will not cause cell deformation to affect staining and morphological effects; the gradient density centrifugation and coarse impurity filtration steps are carried out simultaneously. When performing special staining such as immunohistochemistry, the sample is added in one step to make a glass slide (tissue / cell chip) with samples from multiple preparation areas. The simplification of the above usage process improves efficiency and reduces costs.

[0020] 3. In the pathology slide preparation and staining device based on advanced fluid control technology, the centrifuge motor has not only the centrifugal effect but also the positioning function when adding liquid, and the centrifugation and positioning are completed in one step. The centrifuge motor can be installed above the centrifugal drive component, which further reduces the space of the equipment, and reduces the volume, complexity and production cost of the equipment.

[0021] 4. In the pathology preparation and staining device based on advanced fluid control technology, the liquid adding pipeline is designed based on advanced fluid control technology, which greatly shortens the liquid path stroke, and uses a peristaltic pump to drive the multi-liquid path design to improve efficiency; there is no need for the commonly used three-axis robotic arm, and no need for liquid transfer consumables such as pipettes, etc. The liquid can be directly poured or dripped into the preparation chamber, and multiple liquid paths are operated at the same time, which greatly simplifies the complexity of mechanical movements and reduces the accuracy requirements for motors and liquid pumps. Users can easily replace hoses, which exponentially improves efficiency, improves the efficiency and stability of the equipment, and also reduces the maintenance cost and use cost of the equipment.

[0022] 5. The pipeline form of the pathology slide preparation and staining device based on advanced fluid control technology is that the slide preparation and staining components are large annular pipeline structures, and the slide preparation and staining units can be replaced up and down without stopping the centrifugal drive components. The throughput of the equipment is greatly improved, and the function module of scanning and printing glass slide barcodes, subsequent sealing and scanning function modules, etc. can also be installed to further expand the throughput and functions. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a schematic diagram of the internal overall structure of the present invention;

[0024] Figure 2 It is a schematic diagram of the centrifugal assembly structure and motor installation in the present invention;

[0025] Figure 3 It is a structural schematic diagram of the traditional dyeing film-making and dyeing components plus the lifting plate in the present invention;

[0026] Figure 4 It is a schematic diagram of the structure of the slide preparation and staining components of the immunohistochemistry and special staining in the present invention;

[0027] Figure 5 This is a schematic diagram of the internal structure of the centrifugal test tube in the present invention;

[0028] Figure 6 It is a schematic diagram of the effect of the centrifugal test tube being lifted and dumped to transfer liquid to the film making chamber in the present invention;

[0029] Figure 7 It is a schematic diagram of the effect of gradient centrifugation separation and impurity dumping of the sample in the present invention;

[0030] Figure 8This is a schematic diagram of the effect of sample shaking, breaking up and transferring in the present invention;

[0031] Fig. 9 It is a schematic diagram of the slide preparation chamber-liquid dispenser and glass slide structure, and the filling of sample liquid in the present invention;

[0032] Fig.10 It is a schematic diagram of the film making chamber-liquid dispenser structure cross section and liquid adding and liquid aspiration in the present invention;

[0033] Fig.11 Schematic diagram of the pipeline form in the present invention

[0034] The meaning of each number in the figure is:

[0035] 1. Centrifugal assembly; 11. Centrifugal drive component; 101. Centrifugal fluid path component; 102. Centrifugal test tube dosing needle; 2. Slide preparation and staining component; 21. Staining turntable; 22. Waste liquid tank; 23. Lifting plate; 201. Conventional staining fluid path component; 202. Immunohistochemistry and special staining fluid path component; 2021. Immunohistochemistry and special staining dosing needle; 2022. Immunohistochemistry and special staining aspiration needle; 2023. Reagent bottle; 2024. Single-head precision dosing needle; 2025. Multi-head precision dosing needle; 2026. Piston; 3. Centrifugal unit; 31. Hanging basket; 32. Centrifugal test tube; 321. Sleeve; 322. Filter; 323, sampling brush; 324, gradient density separation liquid; 325, sample cells; 326, upper impurities; 327, cell suspension; 328, vibration motor; 329, diluent; 4, slide making and staining unit; 5, glass slide; 51, traditional staining glass slide; 52, special staining special glass slide; 53, slide making area; 6, slide making chamber; 61, traditional staining special slide making chamber; 62, slide making chamber-liquid dispenser; 621, liquid reservoir; 622, overflow port; 623, flow channel; 624, channel; 625, steam hole; 626, water bath; 7, centrifugal positioning motor; 8, staining positioning motor; 9, lifting motor. DETAILED DESCRIPTION

[0036] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] The present invention provides a pathology slide preparation and staining device based on advanced fluid control technology, the device adopts an upper and lower layered design, such as Figure 1-Figure 11As shown, it includes an upper centrifugal assembly 1 and a lower film-making and dyeing assembly 2, the centrifugal assembly 1 includes a centrifugal drive component 11 and an upper centrifugal liquid path component, the film-making and dyeing assembly 2 includes an arc-shaped dyeing turntable component 21 and an upper dyeing liquid path component; a plurality of film-making and dyeing units 4 and a plurality of waste liquid tanks 22 are arranged on the dyeing turntable 21; the liquid after centrifugation of the centrifugal assembly 1 is directly poured and transferred to the film-making and dyeing assembly 2 or the waste liquid tank 22 below. Through advanced fluid control technology, the present invention cleverly combines the centrifugal assembly with the film-making and dyeing assembly to achieve precise control and efficient transfer of liquid. This layout not only saves space, but also makes the entire device more compact and reasonable. At the same time, the arc-shaped dyeing turntable design facilitates the arrangement and mechanical action of the film-making and dyeing units and the waste liquid tank, thereby improving work efficiency. This design can complete multiple liquid transfer operations in one action; after the centrifugal component completes the centrifugal operation, the liquid can be directly poured into the slide preparation and staining component or the waste liquid tank. This process design simplifies the multiple transfer and operation steps in the traditional slide preparation and staining process, which not only improves work efficiency, but also reduces the use of consumables, reduces the cost of use, and also helps to improve the consistency and quality of production.

[0038] The device adopts an upper and lower layered design, with the centrifugal component located at the upper part and the slide-making and dyeing component located at the lower part. This layout not only saves space, but also makes the entire device more compact and reasonable. At the same time, the arc-shaped dyeing turntable design facilitates the arrangement and mechanical movement of the slide-making and dyeing units and the waste liquid tank, thereby improving work efficiency.

[0039] Several waste liquid tanks are arranged on the dyeing turntable, so that a plurality of waste liquids after centrifugation can be directly dumped into the designated positions, which is convenient for the collection and treatment of the waste liquids.

[0040] In this embodiment, a plurality of centrifugal units 3 are annularly installed outside the centrifugal driving component 11 . The centrifugal unit 3 includes a hanging basket 31 . A plurality of centrifugal test tubes 32 are arranged inside the hanging basket 31 .

[0041] Specifically, the upper centrifugal assembly 1 and the lower slide-staining assembly 2 can be controlled by external motors to perform relative up-and-down movement and rotation positioning. The external motors include a centrifugal positioning motor 7, a dyeing positioning motor 8, and a lifting motor 9. The dyeing positioning motor 8 controls the rotation positioning, so that the slide-staining unit 4 or the waste liquid tank 22 and the centrifugal unit 3 are positioned one by one. A lifting plate 23 is installed on the dyeing turntable 21, and the lifting plate 23 is located below the centrifugal unit 3. The centrifugal assembly 1 and the slide-staining assembly move up and down relative to each other, so that the lifting plate 23 lifts the centrifugal unit 3, and the centrifugal unit 3 is turned over to directly dump the liquid in the centrifugal test tube into the slide-staining unit 4 or the waste liquid tank 22. The liquid after the centrifugal drive assembly 1 completes the centrifugation is directly dumped into the slide-staining assembly 2 or the waste liquid tank 22 below. In addition to the function of centrifuging the sample, the centrifugal drive assembly 1 also has the function of shaking and breaking up the sample by shaking or vibrating the motor 328 back and forth.

[0042] Furthermore, the centrifugal test tube 32 can also be used as a cell sample preservation liquid bottle; a sleeve 321 is added close to the inner wall of the centrifugal test tube 32, the lower end outlet of the sleeve 321 is close to the bottom of the centrifugal test tube 32, and a filter 322 is installed. The filter 322 is close to the bottom of the centrifugal test tube 32. When sampling, the sampling brush is placed on the outside of the sleeve 321, and the tip of the sampling brush 323 or an alternative stirring object (such as a suction head of appropriate size) is facing the bottom of the centrifugal test tube. A vibration motor 328 is installed below the centrifugal test tube 32, and the two cooperate to shake and stir, so as to better break up the cell sample and suspend it. Add gradient density separation liquid before centrifugation, and slowly enter the bottom of the centrifugal test tube 32 from the sleeve 321, so that most of the suspended cells are on the upper part of the separation liquid. Add diluent or buffer after centrifugation and shaking to fully suspend and evenly distribute the cell sample.

[0043] Furthermore, the centrifugal fluid path component includes a centrifugal test tube liquid adding needle, a driving pump and matching pipelines and reagent bottles; the centrifugal test tube liquid adding needle is fixed above the centrifugal unit 3, and the centrifugal driving component 11 is rotated by the centrifugal positioning motor 7 to locate the corresponding liquid adding position.

[0044] Furthermore, the liquid path components of the slide preparation and staining assembly 2 are divided into two types: one is a liquid path component used for traditional staining, such as for Papanicolaou staining and HE staining; the other is a liquid path component specifically used for immunohistochemistry and special staining.

[0045] Furthermore, the slide making and staining unit 4 includes a slide making chamber 6, each of which is provided with a glass slide 5. The slide making chamber 6 is divided into two types, namely, a slide making chamber 61 for traditional staining and a special slide making chamber-dispenser 62 for special staining dedicated to immunohistochemistry and special staining. The glass slides are divided into two categories: traditional staining glass slides 51 and special staining glass slides 52 for immunohistochemistry and the like.

[0046] Furthermore, the conventional staining liquid path component 201 includes a plurality of liquid adding needles, a plurality of waste liquid suction needles, supporting pipelines and drive pumps and reagent bottles / liquid storage bottles. Furthermore, the immunohistochemistry and special staining dedicated liquid path component 202 includes a plurality of dedicated antibody reagent bottles 2023, a single-head precision liquid adding needle 2024, a multi-head precision liquid adding needle 2025; a plurality of immunohistochemistry and special staining liquid suction needles 2022, a plurality of liquid adding needles 2021; other supporting pipelines and drive pumps and reagent bottles / liquid storage bottles; a piston 2026 is set in the antibody reagent bottle 2023, which can also be used as an injection pump.

[0047] Furthermore, the liquid adding drive pump of the centrifugal liquid path component 101, and the liquid adding drive pump and waste liquid suction drive pump of the traditional dyeing liquid path component 201 are all sampling quantitative peristaltic pumps, which are lower in cost and are designed to allow users to easily replace hoses.

[0048] Furthermore, the slide 52 for immunohistochemistry and special staining has a hydrophobic coating to separate several slide adhesion areas 53. The upper layer of the slide chamber-liquid dispenser 62 is provided with a liquid reservoir 621, an overflow port 622 and several flow channels 623. The thickness and length of the flow channels 623 are set according to the flow rate. The end of the flow channel 623 has a downward hole 624. The lower end outlet of the hole 624 is close to the slide area 53. When dripping liquid, it plays the role of multi-porous equal distribution of liquid. The bottom of the slide chamber-liquid dispenser 62 is provided with several steam holes 625, and a water bath 626 is provided below the slide chamber-liquid dispenser 62. The water temperature can be controlled and set, and the water temperature can be transmitted to the slide 52 for immunohistochemistry and special staining and the sample. The steam in the water tank can enter the inside of the slide chamber-liquid dispenser 62 through the steam hole at the bottom of the slide chamber-liquid dispenser 62, which plays the role of keeping the sample warm and moisturizing.

[0049] Furthermore, the slide making and staining component 2 of the present invention can be designed as a large annular assembly line structure, and more slide making and staining units 4 can be installed. The slide making and staining units 4 can be replaced up and down without stopping the centrifugation; in order to further expand the throughput and functions; a function of scanning and printing glass slide barcodes, subsequent sealing and scanning functions, etc. can also be added.

[0050] The pathological slide preparation and staining device based on the advanced fluid control technology of the present invention comprises the following steps when in use:

[0051] 1. Cytology preparation and traditional staining - single machine solution as an example:

[0052] S1. Sample and slide preparation: When sampling, place the sampling brush on the outside of the sleeve, with the tip of the sampling brush or a substitute stirring object facing the tip of the bottom of the centrifuge tube, manually place the cell preservation solution bottle / centrifuge tube, install the filter, install the slide and the slide chamber to the designated position;

[0053] S2, shaking and centrifuging the cell sample: shaking the centrifugal test tube by rotating the centrifugal drive component back and forth to shake and break up the cells in the tube; adding separation liquid through the liquid adding device above, slowly entering from the bottom of the centrifugal test tube, and starting the centrifugal positioning motor for centrifugation;

[0054] S3, sample transfer and sedimentation preparation: lift the preparation staining plate and pour the upper centrifugal waste liquid into the waste liquid tank; add diluent to the centrifugal test tube through the liquid adding device above, start the vibration motor to vibrate the centrifugal test tube, and use a cervical brush or agitator to break up the sample into a cell suspension state;

[0055] S4, sedimentation preparation: lift the preparation staining plate to pour the cell suspension sample in the centrifugal test tube into the preparation chamber below, and leave it for a certain period of time to allow the cells to settle and adhere to the glass slide;

[0056] S5. Traditional staining of cell slides (Papanicolaou staining or HE staining, etc.): Use the waste liquid aspirator to suck away the waste liquid, set the program according to the reagent instructions, add staining solution, rinse solution and aspirate the waste liquid. In the last step, the slide will maintain a certain humidity to prevent drying;

[0057] S6. Take out the stained slide from the slide preparation chamber for subsequent processing such as sealing, scanning or observation.

[0058] 2. Cytological preparation immunohistochemical staining - single machine solution as an example:

[0059] S1. Sample and slide preparation: When sampling, place the sampling brush on the outside of the sleeve, with the tip of the sampling brush or a substitute stirring object facing the tip of the bottom of the centrifuge tube. Manually place the cell preservation solution bottle / centrifuge tube, install the filter, install the slide, and select a suitable immunohistochemistry special preparation chamber-dispenser and install it in the designated position;

[0060] S2-S4 are the same as the steps corresponding to 1 above;

[0061] S5, immunohistochemical staining of cell slides: turn on the water bath in advance and set the temperature according to the reagent instructions; use the waste liquid aspirator to suck away the waste liquid on the slide; set the program according to the reagent instructions, such as: use the reagent bottle and the single-head precision liquid-adding needle to add the primary antibody reagent, incubate at a constant temperature and moisturizing, add the rinse solution and use the multi-head liquid-adding needle to suck out the waste liquid, use the reagent bottle and the multi-head precision liquid-adding needle to add the secondary antibody reagent, incubate at a constant temperature and moisturizing, add the rinse solution and suck out the waste liquid. In the last step, the slide will maintain a certain humidity to prevent drying;

[0062] S6. Take out the stained slide from the slide preparation chamber-dispenser for subsequent sealing, scanning or observation.

[0063] 3. Histological immunohistochemical staining (taking the single-machine solution as an example):

[0064] S1. Prepare the slides manually according to the experimental procedures: such as sample dehydration, embedding, slicing, bleaching, scooping, baking, dewaxing, hydration, antigen repair, etc. Notes on slides and scooping: Use adhesive slides that prevent detachment. The slides have a waterproof coating and are divided into several areas. When scooping, the tissue section sample must fall on the adhesive area within the waterproof isolation zone of the slide.

[0065] S2-S6 are consistent with the corresponding steps in 2 above.

[0066] Finally, it should be noted that the centrifugal positioning motor 7 and the like in this embodiment, the electronic components in the above components are all universal standard parts or components known to those skilled in the art, and their structures and principles are known to those skilled in the art through technical manuals or through conventional experimental methods. In the idle part of this device, all the above electrical components are connected by wires, and the specific connection means should refer to the working sequence between the electrical components in the above working principle to complete the electrical connection, which are all well-known technologies in the art. The centrifugal liquid circuit components include pipe fittings and reagent bottles / liquid storage bottles connected to the centrifugal test tube liquid addition needle and tee; the dyeing liquid circuit components include pipe fittings and reagent bottles / liquid storage bottles and waste liquid bottles connected to the liquid addition needle, liquid aspiration needle, and dyeing needle. The above components are all universal standard parts or components known to those skilled in the art, and their structures and principles are known to those skilled in the art through technical manuals or through conventional experimental methods. In the idle part of this device, all the above components are connected by pipes, and the specific connection means should refer to the working sequence between the accessories in the above working principle to complete the physical connection, which are all well-known technologies in the art.

[0067] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments, and the above embodiments and descriptions are only preferred examples of the present invention, and are not intended to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, and these changes and improvements all fall within the scope of the present invention to be protected.

Claims

1. A pathology slide preparation and staining device based on advanced fluid control technology, comprising an upper centrifugal component (1) and a lower slide preparation and staining component (2), characterized in that: The centrifugal assembly (1) comprises a centrifugal drive component (11) and an upper centrifugal liquid path component (101); the slide-making and dyeing component (2) comprises an arc-shaped dyeing turntable component (21) and an upper dyeing liquid path component; a plurality of slide-making and dyeing units (4) and a plurality of waste liquid tanks (22) are arranged on the dyeing turntable (21); and the liquid after centrifugation of the centrifugal assembly (1) is directly poured and transferred to the slide-making and dyeing component (2) or the waste liquid tank (22) below.

2. The pathological slide preparation and staining device based on advanced fluid control technology according to claim 1, characterized in that: A plurality of centrifugal units (3) are annularly mounted on the outer side of the centrifugal driving component (11). The centrifugal units (3) include a hanging basket (31). A plurality of centrifugal test tubes (32) are arranged inside the hanging basket (31).

3. The pathological slide preparation and staining device based on advanced fluid control technology according to claim 1, characterized in that: The upper centrifugal assembly (1) and the lower slide-making and dyeing assembly (2) can be controlled by an external motor to perform relative up-and-down movement and rotation positioning, so that the slide-making and dyeing unit (4) or the waste liquid tank (22) and the centrifugal unit (3) are located one by one in the upper and lower positions; a lifting plate (23) is installed on the dyeing turntable (21) and is located below the centrifugal unit (3); the lifting plate (23) lifts the centrifugal unit (3) through the relative up-and-down movement, and the centrifugal unit (3) is turned over to directly pour the liquid in the centrifugal test tube (32) therein into the slide-making and dyeing unit (4) or the waste liquid tank (22).

4. The pathological slide preparation and staining device based on advanced fluid control technology according to claim 1, characterized in that: The centrifugal test tube (32) can also be used as a cell sample preservation liquid bottle; a sleeve (321) is added close to the inner wall of the centrifugal test tube (32); the lower end outlet of the sleeve (321) is close to the bottom of the centrifugal test tube (32) and is installed with a filter (322); the filter (322) is close to the bottom of the centrifugal test tube (32); after sampling, a sampling brush (323) or a substitute stirring object is placed outside the sleeve (321), and the tip of the sampling brush (323) or the substitute stirring object is facing the bottom of the centrifugal test tube; a vibration motor (328) is installed below the centrifugal test tube (32).

5. The pathological slide preparation and staining device based on advanced fluid control technology according to claim 1, characterized in that: The centrifugal liquid path component (101) comprises a centrifugal test tube liquid adding needle (102), a driving pump and supporting pipelines and reagent bottles, and a centrifugal positioning motor (7). The centrifugal test tube liquid adding needle (102) is fixed above the centrifugal unit (3). The centrifugal positioning motor (7) can be installed below the centrifugal driving component (11) or above it. The centrifugal driving component (11) is rotated by the centrifugal positioning motor (7) to position the corresponding liquid adding position.

6. The pathological slide preparation and staining device based on advanced fluid control technology according to claim 1, characterized in that: The dyeing liquid path components of the slide making and dyeing assembly (2) are divided into two types: A conventional staining fluid path component (201), such as that used for Papanicolaou staining and HE staining; and another immunohistochemical and special staining fluid path component (202).

7. The pathological slide preparation and staining device based on advanced fluid control technology according to claim 6 is characterized in that: The conventional dyeing liquid path component (201) comprises a plurality of liquid adding needles, a plurality of waste liquid suction needles, matching pipelines, a driving pump and a reagent bottle / liquid storage bottle, a slide making and dyeing unit (4) and a conventional dyeing special slide making chamber (61).

8. The pathological slide preparation and staining device based on advanced fluid control technology according to any one of claims 6 or 7, characterized in that: The immunohistochemistry and special staining liquid path component (202) includes a plurality of immunohistochemistry and special staining liquid adding needles (2021), a plurality of immunohistochemistry and special staining liquid aspiration needles (2022), a plurality of dedicated antibody reagent bottles (2023), a plurality of single-head precision liquid adding needles (2024), and a plurality of multi-head precision liquid adding needles (2025); the slide making and staining unit (4) includes a special staining dedicated glass slide (52), a slide making chamber-liquid dispenser (62), supporting pipelines, a drive pump, and reagent bottles, etc.

9. The pathological slide preparation and staining device based on advanced fluid control technology according to any one of claims 6, 7 or 8, characterized in that: The film making chamber-liquid dispenser (62) plays the role of evenly distributing the liquid to multiple film making areas (53) when dripping liquid. It is placed on the upper part of the special staining dedicated glass slide (52). Its upper layer is provided with a liquid storage tank (621), an overflow port (622) and a plurality of flow channels (623). The thickness and length of the flow channels (623) are set according to the required flow rate. The end of the flow channel (623) is provided with a downward dripping channel (624), and its lower end is closely facing the film making area (53).

10. The pathological slide preparation and staining device based on advanced fluid control technology according to claim 8, characterized in that: A piston (2026) is arranged in the reagent bottle (2023) of the immunohistochemistry and special staining dedicated liquid circuit system, which also serves as an injection pump; the reagent bottle is equipped with a single-head precision liquid addition needle (2024) or a multi-head precision liquid addition needle (2025).

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