Comprehensive Disease-Resistant and Stress-Resistant Cultivation System and Method for Pea Germplasm with Rain-Heat in the Same Season on the Plateau
Through the comprehensive disease-resistant and stress-resistant cultivation system of pea germplasm in the same season of plateau rain heat, pea growth adaptability, disease resistance and stress resistance were evaluated, and the optimal germplasm was screened out, which solved the problem that genetic stability was difficult to ensure quality, and achieved efficient breeding and stable production.
Patent Information
- Application Number
- CN202411697728.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-26
- Publication Date
- 2025-07-18
- Estimated Expiration
- 2044-11-26
AI Technical Summary
The comprehensive disease-resistant and stress-resistant cultivation system of pea germplasm in the middle and upper plateau rain heat season pays more attention to the genetic stability of pea and is difficult to ensure quality.
Germplasm data was obtained through the pea data acquisition module, and the growth cycle matching module and the comprehensive performance analysis module were used to evaluate the growth adaptability, disease resistance and stress resistance of pea, and combined with the pea performance evaluation module to screen out the optimal germplasm.
Rapidly identify potential pea germplasms, shorten breeding cycles, improve pea yield and quality, meet market demand, reduce production risks, and ensure the stability and sustainability of pea production.
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Figure CN119522801B_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of planting, and specifically to a comprehensive disease-resistant and stress-resistant cultivation system and method for pea germplasms with concurrent rain and heat seasons on the plateau. Background Art
[0002] At present, the comprehensive disease-resistant and stress-resistant cultivation of pea germplasms with concurrent rain and heat seasons on the plateau is a very important part of the planting technology field. With the progress of technology and the growth of demand, improving the more refined comprehensive disease-resistant and stress-resistant cultivation system for pea germplasms with concurrent rain and heat seasons on the plateau has become the norm. An efficient and accurate comprehensive disease-resistant and stress-resistant cultivation method for pea germplasms with concurrent rain and heat seasons is of great significance for improving the disease-resistant and stress-resistant abilities, as well as the yield and quality of peas.
[0003] For example, the invention patent with the publication number CN116472956B is a parental combination for screening pea umbrella-shaped inflorescence plants and its application, belonging to the technical field of plant breeding. The pea plants selected by using the method in the present invention have a plant height of 82 - 86 cm, an initial pod height of 40 - 44 cm, an effective pod-bearing length of 41 - 43 cm, 2 branches, 9 - 11 pod-bearing layers, 28 - 32 pods per plant, a pod length of 8.9 - 9.3 cm, 7 - 9 seeds per pod, a 100-seed weight of 22 - 23 g, dark green and wrinkled seeds that do not dehisce, that is, the target plant, the pea umbrella-shaped inflorescence plant, has been successfully screened out. Moreover, only by using the parental combination in the present invention can this target plant be screened out, and other parental combinations cannot.
[0004] For example, the invention patent with the publication number CN118696814A is a pea cross-breeding method in alpine regions, belonging to the technical field of pea breeding; it includes Step 1, pollen preservation; Step 2, emasculation treatment; Step 3, hybridization; Step 4, seed treatment; Step 5, repeated hybridization; Step 6, cultivation. When pollinating in the present invention, by using sodium hypochlorite solution, the influence of microorganisms on pollination can be reduced. The plant extract contains various plant active ingredients that can improve the activity of holly pollen and increase the pollination success rate; through the combined use of a buffer solution, it can not only protect the pollen activity but also avoid its influence by environmental factors such as light, and can also improve the hybridization success rate in alpine environments; through iterative repeated hybridization treatment, it is beneficial for peas to obtain stable excellent genetic traits, further improving the cold resistance of pea plants and ensuring the stability of genetic characteristics in subsequent plantings.
[0005] However, in the process of implementing the technical solutions of the invention in the embodiments of the present application, it is found that the above technologies have at least the following technical problems: Currently, the comprehensive disease-resistant and stress-resistant cultivation system for pea germplasms with concurrent rain and heat seasons on the plateau pays more attention to the genetic stability of peas, and it is difficult to guarantee the quality of peas. Summary of the Invention
[0006] In view of the deficiencies of the prior art, the present invention provides a comprehensive disease-resistant and stress-resistant cultivation system and method for pea germplasms with concurrent rain and heat in the plateau, which can effectively solve the problems involved in the above-mentioned background art.
[0007] To achieve the above objectives, the present invention is realized through the following technical solutions: In the first aspect of the present invention, a comprehensive disease-resistant and stress-resistant cultivation system for pea germplasms with concurrent rain and heat in the plateau is provided, including: a pea data collection module, which is used to obtain pea germplasms with concurrent rain and heat in the plateau, label each pea germplasm, and select sample plants from each pea germplasm for cultivation, and collect the growth and development data of each sample plant of each pea germplasm.
[0008] A growth cycle matching module, which is used to process the growth and development data of each sample plant of each pea germplasm to obtain the growth adaptability evaluation index of each pea germplasm, and match the growth cycle of each pea germplasm according to the growth adaptability evaluation index of each pea germplasm.
[0009] A comprehensive performance analysis module, which is used to collect the cultivation data of each sample plant of each pea germplasm in a preset growth cycle, including disease resistance data and stress resistance data, process the disease resistance data to obtain the pea disease resistance evaluation index of each pea germplasm, process the stress resistance data to obtain the pea stress resistance evaluation index of each pea germplasm, and comprehensively analyze the growth adaptability evaluation index, pea disease resistance evaluation index and pea stress resistance evaluation index of each pea germplasm to obtain the pea comprehensive performance evaluation value of each pea germplasm.
[0010] A pea performance evaluation module, which is used to evaluate the pea performance according to the pea comprehensive performance evaluation value of each pea germplasm, obtain the evaluation result and give feedback.
[0011] As a further method, the process of processing the growth and development data of each sample plant of each pea germplasm to obtain the growth adaptability evaluation index of each pea germplasm is as follows: The growth and development data includes the average leaf area, plant height and number of branches.
[0012] Extract the critical average leaf area, critical plant height and critical number of branches from the pea germplasm database, and comprehensively analyze to obtain the growth adaptability evaluation index of each pea germplasm.
[0013] As a further method, the process of matching the growth cycle of each pea germplasm according to the growth adaptability evaluation index of each pea germplasm is as follows: Input the growth adaptability evaluation index of each pea germplasm into the pea germplasm database to match the growth cycle of each pea germplasm corresponding to the growth adaptability evaluation index range of each pea germplasm, and the growth cycle is the required time for pea development to maturity.
[0014] As a further method, the disease resistance data is processed to obtain the pea disease resistance evaluation index for each pea germplasm. The specific processing process is as follows: The disease resistance data includes the incidence rate, the leaf withering rate, and the average disease resistance enzyme activity.
[0015] Extract the critical incidence rate, the critical leaf withering rate, and the critical average disease resistance enzyme activity from the pea germplasm database, and comprehensively analyze to obtain the pea disease resistance evaluation index for each pea germplasm.
[0016] As a further method, the stress resistance data is processed to obtain the pea stress resistance evaluation index for each pea germplasm. The specific processing process is as follows: The stress resistance data includes the germination rate, the chlorophyll content, and the malondialdehyde content.
[0017] Extract the critical germination rate, the critical chlorophyll content, and the critical malondialdehyde content from the pea germplasm database, and comprehensively analyze to obtain the pea stress resistance evaluation index for each pea germplasm.
[0018] As a further method, the comprehensive performance evaluation value of each pea germplasm is obtained through comprehensive analysis. The specific analysis process is as follows: According to the growth adaptability evaluation index, the pea disease resistance evaluation index, and the pea stress resistance evaluation index of each pea germplasm, comprehensively analyze to obtain the comprehensive performance evaluation value of each pea germplasm. The comprehensive performance evaluation value of each pea germplasm is used to quantify the comprehensive performance of each pea germplasm.
[0019] As a further method, the performance of peas is evaluated according to the comprehensive performance evaluation value of each pea germplasm. The specific evaluation process is as follows: Extract the comprehensive performance evaluation threshold from the pea germplasm database, compare the comprehensive performance evaluation value of each pea germplasm with the comprehensive performance evaluation threshold. If the comprehensive performance evaluation value of a certain pea germplasm is greater than or equal to the comprehensive performance evaluation threshold, then evaluate this pea germplasm as qualified; if the comprehensive performance evaluation value of a certain pea germplasm is less than the comprehensive performance evaluation threshold, then evaluate this pea germplasm as unqualified, and count the number of qualified pea germplasms.
[0020] If the number of qualified pea germplasms is zero, then re-cultivate the pea germplasm; if the number of qualified pea germplasms is not zero, then screen out the optimal pea germplasm.
[0021] As a further method, the optimal pea germplasm is screened out. The specific screening process is as follows: Sort the comprehensive performance evaluation values of each qualified pea germplasm in descending order, and mark the pea germplasm with the largest comprehensive performance evaluation value as the optimal pea germplasm.
[0022] As a further method, the pea disease resistance evaluation index of each pea germplasm, the specific numerical expression is:
[0023]
[0024] Among them, Dr i represents the pea disease resistance evaluation index of the i-th pea germplasm, d i represents the incidence rate of the i-th pea germplasm, d0 represents the critical incidence rate, e represents the natural constant, K ij represents the leaf withering rate of the j-th sample plant of the i-th pea germplasm, K0 represents the critical leaf withering rate, M ij represents the average disease-resistant enzyme activity of the j-th sample plant of the i-th pea germplasm, M0 represents the critical average disease-resistant enzyme activity, ρ1 represents the pea disease resistance evaluation influencing factor corresponding to the set incidence rate, ρ2 represents the pea disease resistance evaluation influencing factor corresponding to the set leaf withering rate, ρ3 represents the pea disease resistance evaluation influencing factor corresponding to the set average disease-resistant enzyme activity, i represents the number of each pea germplasm, i = 1, 2, 3,..., m, m represents the total number of pea germplasms, j represents the number of each sample plant, j = 1, 2, 3,..., n, and n represents the total number of sample plants.
[0025] The second aspect of the present invention provides a comprehensive disease and stress resistance cultivation method for high-altitude peas with concurrent rain and heat seasons, including: obtaining high-altitude peas with concurrent rain and heat seasons, labeling them as each pea germplasm, selecting sample plants from each pea germplasm for cultivation, and collecting the growth and development data of each sample plant of each pea germplasm.
[0026] Processing the growth and development data of each sample plant of each pea germplasm to obtain the growth adaptability evaluation index of each pea germplasm, and matching the growth cycle of each pea germplasm according to the growth adaptability evaluation index of each pea germplasm.
[0027] Collecting the cultivation data of each sample plant of each pea germplasm in the preset growth cycle, including disease resistance data and stress resistance data, processing the disease resistance data to obtain the pea disease resistance evaluation index of each pea germplasm, processing the stress resistance data to obtain the pea stress resistance evaluation index of each pea germplasm, and comprehensively analyzing the growth adaptability evaluation index, pea disease resistance evaluation index, and pea stress resistance evaluation index of each pea germplasm to obtain the pea comprehensive performance evaluation value of each pea germplasm.
[0028] Evaluating the pea performance according to the pea comprehensive performance evaluation value of each pea germplasm, obtaining the evaluation result and giving feedback.
[0029] Compared with the prior art, the embodiments of the present invention at least have the following advantages or beneficial effects:
[0030] (1) By providing a comprehensive disease-resistant and stress-resistant cultivation system and method for pea germplasms with concurrent rain and heat in the plateau, the present invention can quickly identify potential pea germplasms, thereby shortening the breeding cycle, accelerating the promotion and application of new varieties, helping to meet the market demand for high-quality peas, and at the same time providing a scientific basis for farmers' planting decisions, further reducing production risks, and ensuring the stability and sustainability of pea production.
[0031] (2) By evaluating the growth adaptability evaluation index of each pea germplasm, the present invention can screen out varieties that are more suitable for specific regions and cultivation requirements, provide strong support for the improvement and renewal of pea varieties, optimize the growth space of pea plants, improve the light energy utilization rate and nutrient utilization efficiency, thereby further increasing the yield and quality and meeting the market demand.
[0032] (3) By evaluating the pea stress resistance evaluation index of each pea germplasm, the present invention can screen out pea varieties with strong stress resistance, help breed pea varieties with different stress resistance characteristics, thereby enriching pea germplasm resources and promoting the diversified development of varieties, and can also take targeted management measures for specific adversity conditions to improve the utilization efficiency of resources. BRIEF DESCRIPTION OF THE DRAWINGS
[0033] The present invention will be further described with reference to the accompanying drawings. However, the embodiments in the drawings do not constitute any limitation to the present invention. For those of ordinary skill in the art, other drawings can be obtained based on the following drawings without creative efforts.
[0034] Figure 1 It is a schematic diagram of the connection of system modules of the present invention.
[0035] Figure 2 It is a schematic diagram of the method flow of the present invention.
[0036] Figure 3 It is a schematic diagram of the functional relationship between the comprehensive performance evaluation value of peas of the present invention and the pea stress resistance evaluation index. DETAILED DESCRIPTION OF THE EMBODIMENTS
[0037] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts belong to the scope of protection of the present invention.
[0038] Refer to Figure 1As shown in the figure, the first aspect of the present invention provides a comprehensive disease-resistant and stress-resistant cultivation system for high-altitude pea germplasm with simultaneous rain and heat seasons, including: a pea data collection module, which is used to obtain high-altitude pea germplasm with simultaneous rain and heat seasons, label each pea germplasm, select sample plants from each pea germplasm for cultivation, and collect the growth and development data of each sample plant of each pea germplasm.
[0039] A growth cycle matching module, which is used to process the growth and development data of each sample plant of each pea germplasm to obtain the growth adaptability evaluation index of each pea germplasm, and match the growth cycle of each pea germplasm according to the growth adaptability evaluation index of each pea germplasm.
[0040] A comprehensive performance analysis module, which is used to collect the cultivation data of each sample plant of each pea germplasm in a preset growth cycle, including disease resistance data and stress resistance data, process the disease resistance data to obtain the pea disease resistance evaluation index of each pea germplasm, process the stress resistance data to obtain the pea stress resistance evaluation index of each pea germplasm, and comprehensively analyze the growth adaptability evaluation index, pea disease resistance evaluation index and pea stress resistance evaluation index of each pea germplasm to obtain the pea comprehensive performance evaluation value of each pea germplasm.
[0041] A pea performance evaluation module, which is used to evaluate the pea performance according to the pea comprehensive performance evaluation value of each pea germplasm, obtain the evaluation result and give feedback.
[0042] Specifically, processing the growth and development data of each sample plant of each pea germplasm to obtain the growth adaptability evaluation index of each pea germplasm, the specific processing process is: the growth and development data includes the average leaf area, plant height and the number of branches.
[0043] Extract the critical average leaf area, critical plant height and critical number of branches from the pea germplasm database, and comprehensively analyze to obtain the growth adaptability evaluation index of each pea germplasm.
[0044] In a specific embodiment, the average leaf area is an important indicator for measuring the photosynthetic capacity of pea plants. A larger leaf area can capture more light energy, thereby improving the photosynthesis efficiency and providing more energy and nutrients for the plants. Therefore, by evaluating the average leaf area, the photosynthetic capacity and growth potential of pea germplasms can be indirectly understood. The leaf area of each leaf can be obtained by a leaf area meter, and the average leaf area can be obtained by summing up the leaf areas of all leaves and dividing by the total number of leaves. The plant height is an important indicator for measuring the growth space utilization ability of pea plants. Taller plants can better utilize the light energy in the upper space, thereby improving the light energy utilization rate and yield. Therefore, by evaluating the plant height, the growth space utilization ability of pea germplasms can be understood, and the plant height can be directly measured with a tape measure. The number of branches is an important indicator for measuring the growth vigor and yield potential of pea plants. More branches mean more growth points and pod-bearing parts, which may increase the yield. Therefore, by evaluating the number of branches, the growth vigor and yield potential of pea germplasms can be understood, and the number of branches can be obtained by counting.
[0045] Furthermore, the growth adaptability evaluation index of each pea germplasm has the following specific numerical expression:
[0046]
[0047] where Ga i represents the growth adaptability evaluation index of the i-th pea germplasm, e represents the natural constant, S ij represents the average leaf area of the j-th sample plant of the i-th pea germplasm, S0 represents the critical average leaf area, h ij represents the height of the j-th sample plant of the i-th pea germplasm, h0 represents the critical plant height, Z ij represents the number of branches of the j-th sample plant of the i-th pea germplasm, Z0 represents the critical number of branches, ω1 represents the growth adaptability evaluation influence factor corresponding to the set average leaf area, ω2 represents the growth adaptability evaluation influence factor corresponding to the set plant height, ω3 represents the growth adaptability evaluation influence factor corresponding to the set number of branches, i represents the numbers of each pea germplasm, i = 1, 2, 3,..., m, m represents the total number of pea germplasms, j represents the numbers of each sample plant, j = 1, 2, 3,..., n, and n represents the total number of sample plants.
[0048] The algorithm of this embodiment combines the average leaf area, plant height, and number of branches of each sample plant of each pea germplasm, and comprehensively analyzes to obtain the growth adaptability evaluation index of each pea germplasm. There is often a positive correlation trend between the height and the number of branches of pea plants. That is to say, taller plants tend to have more branches. This may be because taller plants can utilize light energy more effectively, promoting the growth and development of lateral branches; there may not be a direct linear relationship between the average leaf area and plant height, but the two may be indirectly related through other factors such as photosynthesis efficiency and nutrient distribution. Taller plants may have a larger leaf area, thus being able to capture more light energy for photosynthesis; in some cases, the average leaf area and the number of branches may show a trend of synergistic growth. That is to say, as the number of branches increases, the average leaf area may also increase accordingly. This may be because the increase in branches promotes the growth and development of leaves. Through comprehensive analysis, a more comprehensive, accurate, and in-depth growth adaptability evaluation index can be obtained.
[0049] Table 1 Example data of the growth adaptability evaluation index of each pea germplasm
[0050]
[0051]
[0052] As shown in Table 1, the growth adaptability evaluation index of each pea germplasm is jointly determined by the average leaf area, plant height, and number of branches of each sample plant of each pea germplasm. In a specific embodiment, n = 1, and the critical average leaf area is 8 cm 2, the critical plant height is 2m, the critical number of branches is 10, the growth adaptability evaluation influence factor corresponding to the set average leaf area is 0.3, the growth adaptability evaluation influence factor corresponding to the set plant height is 0.4, and the growth adaptability evaluation influence factor corresponding to the set number of branches is 0.3. This formula takes into account three key factors, namely average leaf area, plant height, and number of branches, and can screen out germplasms with excellent traits, which helps to accelerate the breeding process, improve breeding efficiency, and can also optimize and rationally allocate germplasm resources according to their growth characteristics and yield potential, thus promoting the sustainable development of the pea industry. It can also formulate more reasonable cultivation management measures, such as irrigation, fertilization, pruning, etc., to further improve the growth rate and yield of pea plants. By standardizing the average leaf area, plant height, and number of branches, it ensures that they are compared on the same scale, improving the fairness and comparability of the evaluation. At the same time, the setting of S0, h0, and Z0 helps to more effectively utilize land resources, water resources, and nutrient resources, improving production efficiency. By weighting the influences of the average leaf area, plant height, and number of branches, it reflects their relative importance in the evaluation index, and the weights of different factors can be adjusted according to different needs, making the model highly adaptable. It is not difficult to see that the larger the average leaf area, plant height, or number of branches, the larger the growth adaptability evaluation index of each pea germplasm. By evaluating the growth adaptability evaluation index of each pea germplasm, varieties more suitable for specific regions and cultivation requirements can be screened out, providing strong support for the improvement and update of pea varieties. It can also optimize the growth space of pea plants, improve the light energy utilization rate and nutrient utilization efficiency, thereby further increasing the yield and quality, helping to predict the yield potential of different varieties, providing a scientific basis for yield prediction for farmers and agricultural enterprises, helping them formulate more reasonable production plans and sales strategies, and further ensuring the stable and reliable quality of pea products to meet market demands.
[0053] It should be understood that in this embodiment, ω1, ω2, and ω3 are respectively the growth adaptability evaluation influence factors corresponding to the preset average leaf area, plant height, and number of branches in the pea germplasm database, representing the numerical values of the influence degrees of the average leaf area, plant height, and number of branches on the growth adaptability evaluation index of the pea germplasm. They can be directly obtained from the pea germplasm database during use, and their corresponding relationships can be pre-set mapping relationships. For example, the average leaf area, plant height, and number of branches of each sample plant of each pea germplasm form a mapping set with the growth adaptability evaluation influence factors corresponding to the preset average leaf area, plant height, and number of branches in the pea germplasm database. Inputting the real-time average leaf area, plant height, and number of branches into the mapping set to obtain the growth adaptability evaluation influence factors corresponding to the average leaf area, plant height, and number of branches, and the mapping relationships therein can be one-to-one or many-to-one relationships. In this example, their value ranges are between 0 and 1.
[0054] It should be noted that the growth adaptability evaluation index of each pea germplasm in this embodiment is a quantitative index obtained by analyzing the average leaf area, plant height, and number of branches of each sample plant of each pea germplasm, and is used to quantify the growth adaptation degree of each pea germplasm.
[0055] Furthermore, the growth cycle of each pea germplasm is matched according to the growth adaptability evaluation index of each pea germplasm. The specific matching process is as follows: The growth adaptability evaluation index of each pea germplasm is input into the pea germplasm database to match the growth cycle of each pea germplasm corresponding to the growth adaptability evaluation index interval of each pea germplasm. The growth cycle is the required time for pea development to maturity.
[0056] Specifically, the pea disease resistance evaluation index of each pea germplasm is obtained by processing the disease resistance data. The specific processing process is as follows: The disease resistance data includes the incidence rate, leaf withering rate, and average disease resistance enzyme activity.
[0057] The critical incidence rate, critical leaf withering rate, and critical average disease resistance enzyme activity are extracted from the pea germplasm database, and the pea disease resistance evaluation index of each pea germplasm is obtained through comprehensive analysis.
[0058] In a specific embodiment, the incidence rate refers to the proportion of diseased plants to the total number of plants. The incidence rate is an important indicator for evaluating the health status of pea plants. By monitoring the incidence rate, early symptoms of diseases can be detected in a timely manner, providing a warning for disease prevention and control, and can be obtained by statistical calculation. The leaf withering rate refers to the proportion of withered leaves to the total number of leaves. The leaf withering rate is an intuitive indicator for evaluating the water status and health status of pea plants. Excessive leaf withering usually means that the plants have suffered severe stress such as drought, disease, or nutrient deficiency. By monitoring the leaf withering rate, field management measures such as irrigation and fertilization can be guided, and can be obtained by statistical calculation. The average disease resistance enzyme activity refers to the average value of the activities of superoxide dismutase, peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase. The disease resistance enzyme activity is an important defense line for pea plants to resist disease invasion. By monitoring the disease resistance enzyme activity, the physiological state of the plants can be understood in a timely manner, thereby protecting the integrity of cell structure and function, and can be obtained by calculating (ΔA / min) / ε×V, where ΔA is the change in absorbance per unit time, which can be measured by a spectrophotometer, ε is the molar extinction coefficient of the product, which is a constant, usually obtained through experimental determination or literature review, and V is the reaction volume, which can be measured by a volume measuring device.
[0059] Furthermore, the specific numerical expression of the pea disease resistance evaluation index of each pea germplasm is:
[0060]
[0061] Among them, Dr i represents the pea disease resistance evaluation index of the i-th pea germplasm, d i represents the incidence rate of the i-th pea germplasm, d0 represents the critical incidence rate, e represents the natural constant, K ij represents the leaf withering rate of the j-th sample plant of the i-th pea germplasm, K0 represents the critical leaf withering rate, M ij represents the average disease-resistant enzyme activity of the j-th sample plant of the i-th pea germplasm, M0 represents the critical average disease-resistant enzyme activity, ρ1 represents the pea disease resistance evaluation influencing factor corresponding to the set incidence rate, ρ2 represents the pea disease resistance evaluation influencing factor corresponding to the set leaf withering rate, ρ3 represents the pea disease resistance evaluation influencing factor corresponding to the set average disease-resistant enzyme activity, i represents the number of each pea germplasm, i = 1, 2, 3,..., m, m represents the total number of pea germplasms, j represents the number of each sample plant, j = 1, 2, 3,..., n, and n represents the total number of sample plants.
[0062] The algorithm of this embodiment combines the incidence rate, leaf withering rate and average disease-resistant enzyme activity of each pea germplasm, and comprehensively analyzes to obtain the pea disease resistance evaluation index. There is often a certain positive correlation between the incidence rate and the leaf withering rate. When the pea germplasm is invaded by diseases, the incidence rate increases, and at the same time, the leaf withering rate increases. This is because the disease will damage the cell structure of the leaves, resulting in the leaves being unable to carry out photosynthesis and nutrient absorption normally and finally withering; when the pea germplasm is invaded by diseases, the activity of disease-resistant enzymes in its body usually changes, and the activity of some enzymes related to disease resistance may increase to cope with the invasion of diseases, thereby reducing the incidence rate; the level of disease-resistant enzyme activity can affect the speed and degree of the leaf withering rate. If the disease-resistant enzyme activity is high, it may be able to protect the leaf cells from the invasion of diseases or slow down the development speed of the diseases, thereby reducing the leaf withering rate. Through comprehensive analysis, a more comprehensive, accurate and in-depth pea disease resistance evaluation index can be obtained.
[0063] It should be noted that in this embodiment, three key factors are considered, namely the incidence rate of each pea germplasm, the leaf withering rate, and the average disease-resistant enzyme activity. These can comprehensively evaluate the disease-resistant performance of pea germplasms, provide an important basis for disease-resistant breeding, and can also predict the occurrence of diseases in different germplasms under specific environments, so as to take corresponding preventive measures, such as reasonable fertilization, scientific irrigation, and strengthening field management, etc., to reduce the probability of disease occurrence. By regulating environmental conditions such as temperature, humidity, light, etc., or using measures such as biological pesticides and chemical pesticides, the level of disease-resistant enzyme activity in peas can be induced or increased, thereby enhancing the disease resistance of peas and providing rich genetic resources for disease-resistant breeding. By standardizing the incidence rate, leaf withering rate, and average disease-resistant enzyme activity of each pea germplasm, it is ensured that they are compared on the same scale, improving the fairness and comparability of the evaluation. At the same time, the setting of d0, K0, and M0 helps to timely monitor and control the spread of pea diseases, so as to take measures for prevention and control in a timely manner. By weighting the effects of the incidence rate, leaf withering rate, and average disease-resistant enzyme activity of each pea germplasm, their relative importance in the evaluation index is reflected, and the weights of different factors can be adjusted according to different needs, making the formula have good adaptability. It is not difficult to see that when the incidence rate of each pea germplasm is smaller, or the leaf withering rate is smaller, or the average disease-resistant enzyme activity is larger, the pea disease resistance evaluation index of each pea germplasm is larger. By evaluating the pea disease resistance evaluation index of each pea germplasm, it helps to screen out germplasms with excellent disease-resistant traits, reduce the impact of diseases on pea yield and quality, can more targeted select disease-resistant parents for cross-breeding, so as to breed new varieties with excellent disease resistance, and can also timely discover and promote new varieties with excellent disease resistance, thereby accelerating the renewal and replacement of varieties, and at the same time can reduce the use of pesticides, thereby reducing the pesticide residues in peas and ensuring food safety and the health of consumers, further enhancing market competitiveness.
[0064] It should be understood that in this embodiment, ρ1, ρ2, and ρ3 are respectively the pea disease resistance evaluation influencing factors corresponding to the preset incidence rate, leaf withering rate, and average disease-resistant enzyme activity in the pea germplasm database, representing the numerical values of the influence degrees of the incidence rate, leaf withering rate, and average disease-resistant enzyme activity on the pea disease resistance evaluation index. They can be directly obtained from the pea germplasm database during use, and their corresponding relationships can be pre-set mapping relationships. For example, the incidence rate, leaf withering rate, and average disease-resistant enzyme activity of each pea germplasm form a mapping set with the pea disease resistance evaluation influencing factors corresponding to the preset incidence rate, leaf withering rate, and average disease-resistant enzyme activity in the pea germplasm database. Inputting the real-time incidence rate, leaf withering rate, and average disease-resistant enzyme activity into the mapping set to obtain the pea disease resistance evaluation influencing factors corresponding to the incidence rate, leaf withering rate, and average disease-resistant enzyme activity. The mapping relationships therein can be one-to-one or many-to-one relationships. In this example, their value ranges are between 0 and 1.
[0065] It should be explained that the pea disease resistance evaluation index of each pea germplasm in this embodiment is a quantitative index obtained by analyzing the incidence rate, leaf withering rate and average disease resistance enzyme activity of each pea germplasm, and is used to quantify the disease resistance performance of each pea germplasm.
[0066] Specifically, the pea stress resistance evaluation index of each pea germplasm is obtained by processing the stress resistance data. The specific processing process is as follows: the stress resistance data includes germination rate, chlorophyll content and malondialdehyde content.
[0067] Extract the critical germination rate, critical chlorophyll content and critical malondialdehyde content from the pea germplasm database, and comprehensively analyze to obtain the pea stress resistance evaluation index of each pea germplasm.
[0068] In a specific embodiment, the germination rate refers to the ratio of the number of germinated seeds to the total number of seeds. The germination rate is an important index to measure the germination ability of pea seeds, which is directly related to the emergence rate and field performance of seeds. Pea seeds with a high germination rate can ensure neat emergence in the field and improve crop yield, and can be obtained by recording observations and statistical calculations; the chlorophyll content refers to the total chlorophyll content. Chlorophyll is a key pigment for plants to carry out photosynthesis, and its content directly affects the photosynthesis and growth and development of plants. Pea germplasms with a high chlorophyll content usually have stronger photosynthesis ability and growth potential, which is beneficial to the improvement of crop yield and quality, and can be calculated by (20.21×A645 + 8.02×A663)×Vext×D÷m÷1000, where A645 and A663 respectively represent the absorbance values at wavelengths of 645 nm and 663 nm, which are measured by a spectrophotometer; Vext represents the volume of the extraction solution, which can be measured by a container; D represents the dilution factor, which can be directly recorded; m represents the sample mass, which can be obtained by weighing; malondialdehyde is a lipid peroxidation product produced by plant cells under stress. The increase in its content usually means that the cells are damaged. By measuring the malondialdehyde content, the stress resistance of pea germplasms, such as drought resistance and heat resistance, can be evaluated, and the malondialdehyde content can be calculated by (malondialdehyde concentration × sample volume) / (sample mass × molar mass of malondialdehyde), where the malondialdehyde concentration can be obtained by gas chromatography, the sample volume can be measured by a volumetric measuring device, the sample mass can be measured by a weighing device, and the molar mass of malondialdehyde can be directly obtained.
[0069] Furthermore, the specific numerical expression of the pea stress resistance evaluation index of each pea germplasm is:
[0070]
[0071] Among them, Sr iDenote the pea stress resistance evaluation index of the \(i\)-th pea germplasm, \(L\). i Denote the germination rate of the \(i\)-th pea germplasm, \(L_0\) denotes the critical germination rate, \(g\). ij Denote the chlorophyll content of the \(j\)-th sample plant of the \(i\)-th pea germplasm, \(g_0\) denotes the critical chlorophyll content, \(D\). ij Denote the malondialdehyde content of the \(j\)-th sample plant of the \(i\)-th pea germplasm, \(D_0\) denotes the critical malondialdehyde content. Denote the influence factor of pea stress resistance evaluation corresponding to the set germination rate. Denote the influence factor of pea stress resistance evaluation corresponding to the set chlorophyll content. Denote the influence factor of pea stress resistance evaluation corresponding to the set malondialdehyde content. \(i\) denotes the number of each pea germplasm, \(i = 1, 2, 3, \cdots, m\), \(m\) denotes the total number of pea germplasms, \(j\) denotes the number of each sample plant, \(j = 1, 2, 3, \cdots, n\), \(n\) denotes the total number of sample plants.
[0072] The algorithm of this embodiment combines the germination rate, chlorophyll content and malondialdehyde content of each pea germplasm, and comprehensively analyzes to obtain the pea stress resistance evaluation index of each pea germplasm. There may be a certain positive correlation between the germination rate of pea seeds and their chlorophyll content. Chlorophyll is the key pigment for plants to carry out photosynthesis, and its content directly affects the photosynthesis and growth and development of plants. Pea seeds with high chlorophyll content may have stronger photosynthesis ability and growth potential, which is beneficial to seed germination and seedling growth. There may be a certain negative correlation between the germination rate of pea seeds and the malondialdehyde content. Malondialdehyde is a lipid peroxidation product produced by plant cells under stress such as drought, high temperature, low temperature, etc. The increase in its content usually means that the cells are damaged. Under stress conditions such as drought stress, the malondialdehyde content of pea seeds may increase, which may inhibit seed germination and seedling growth, thereby reducing the germination rate. There may be some association between the chlorophyll content and the malondialdehyde content, but this association may vary depending on the stress environment. For example, under drought stress, the chlorophyll content of pea leaves may decrease while the malondialdehyde content may increase. This may be because drought stress damages plant cells, affecting the synthesis and degradation processes of chlorophyll, and at the same time promoting the production of lipid peroxidation reactions. However, in other stress environments such as high temperature, low temperature, salt stress, etc., the association between the chlorophyll content and the malondialdehyde content may show different trends. Comprehensive analysis can obtain a more comprehensive, accurate and in-depth pea stress resistance evaluation index.
[0073] It should be noted that in this embodiment, three key factors are considered, namely the germination rate, chlorophyll content, and malondialdehyde content of each pea germplasm. This can comprehensively evaluate the quality of pea germplasms, provide a scientific basis for breeding high-yield, high-quality, and stress-resistant pea varieties, and also formulate targeted planting strategies. For example, for pea germplasms with high germination rates and high chlorophyll contents, management measures such as close planting and high fertilization can be adopted to fully exert their growth potential. It can also more accurately locate target genes, improve breeding efficiency, help to discover germplasm resources with potential utilization value, and provide new material sources for pea breeding. By standardizing the germination rate, chlorophyll content, and malondialdehyde content of each pea germplasm, ensuring that they are compared on the same scale, the fairness and comparability of the evaluation are improved. At the same time, the setting of L0, g, and D0 helps to screen out excellent pea germplasms with high germination rates, high chlorophyll contents, and low malondialdehyde contents, thereby increasing yield and quality. By weighting the effects of the germination rate, chlorophyll content, and malondialdehyde content of each pea germplasm, their relative importance in the evaluation index is reflected, and the weights of different factors can be adjusted according to different needs, making the formula highly adaptable. It is not difficult to see that when the germination rate, chlorophyll content, or malondialdehyde content of each pea germplasm is larger or smaller, respectively, the pea stress resistance evaluation index of each pea germplasm is larger. By evaluating the pea stress resistance evaluation index of each pea germplasm, pea varieties with strong stress resistance can be screened out, which helps to breed pea varieties with different stress resistance characteristics, thus enriching pea germplasm resources and promoting the diversified development of varieties. It can also take targeted management measures for specific adverse conditions, such as irrigation, fertilization, shading, etc., to improve the stress resistance and yield of peas, further more effectively cope with natural disasters and reduce the impact of disasters on agricultural production. It can help farmers make more effective use of resources such as water and fertilizers, reduce waste and pollution of resources, contribute to the green development of agriculture, and improve the utilization efficiency of resources.
[0074] It should be understood that in this embodiment and They are the influencing factors for evaluating the stress resistance of peas corresponding to the preset germination rate, chlorophyll content, and malondialdehyde content in the pea germplasm database, which are the numerical values indicating the influence degrees of the germination rate, chlorophyll content, and malondialdehyde content on the stress resistance evaluation index of peas. They can be directly obtained from the pea germplasm database during use, and their corresponding relationships can be pre-set mapping relationships. For example, the germination rate, chlorophyll content, and malondialdehyde content of each pea germplasm form a mapping set with the influencing factors for evaluating the stress resistance of peas corresponding to the preset germination rate, chlorophyll content, and malondialdehyde content in the pea germplasm database. Inputting the real-time germination rate, chlorophyll content, and malondialdehyde content into the mapping set to obtain the influencing factors for evaluating the stress resistance of peas corresponding to the germination rate, chlorophyll content, and malondialdehyde content. The mapping relationships therein can be one-to-one or many-to-one relationships. In this example, their value ranges are from 0 to 1.
[0075] It should be noted that the stress resistance evaluation index of each pea germplasm in this embodiment is a quantitative index obtained by analyzing the germination rate, chlorophyll content, and malondialdehyde content of each pea germplasm, and is used to quantify the stress resistance performance of each pea germplasm.
[0076] Specifically, the comprehensive performance evaluation value of each pea germplasm is obtained through comprehensive analysis. The specific analysis process is as follows: According to the growth adaptability evaluation index, pea disease resistance evaluation index, and pea stress resistance evaluation index of each pea germplasm, the comprehensive performance evaluation value of each pea germplasm is obtained through comprehensive analysis. The comprehensive performance evaluation value of each pea germplasm is used to quantify the comprehensive performance of each pea germplasm.
[0077] Furthermore, the specific numerical expression of the comprehensive performance evaluation value of each pea germplasm is:
[0078] Cp i =tanh(θ1*Ga i +θ2*Dr i +θ3*Sr i );
[0079] Among them, Cp i represents the comprehensive performance evaluation value of the i-th pea germplasm, Ga i represents the growth adaptability evaluation index of the i-th pea germplasm, Dr i represents the pea disease resistance evaluation index of the i-th pea germplasm, Sr i represents the pea stress resistance evaluation index of the i-th pea germplasm, θ1 represents the influencing factor for evaluating the comprehensive performance of peas corresponding to the set growth adaptability evaluation index, θ2 represents the influencing factor for evaluating the comprehensive performance of peas corresponding to the set pea disease resistance evaluation index, θ3 represents the influencing factor for evaluating the comprehensive performance of peas corresponding to the set pea stress resistance evaluation index, i represents the number of each pea germplasm, i = 1, 2, 3,..., m, and m represents the total number of pea germplasms.
[0080] As Figure 3 shown, in a specific embodiment, m = 1, θ1 = 0.3, θ2 = θ3 = 0.4. When Ga i = Dr i = 0.1, the functional relationship between the comprehensive performance evaluation value of peas and the stress resistance evaluation index of peas is shown as curve a; when Ga i = Dr i = 0.5, the functional relationship between the comprehensive performance evaluation value of peas and the stress resistance evaluation index of peas is shown as curve b; when Ga i = Dr i = 1, the functional relationship between the comprehensive performance evaluation value of peas and the stress resistance evaluation index of peas is shown as curve c.
[0081] The algorithm of this embodiment combines the growth adaptability evaluation index, disease resistance evaluation index and stress resistance evaluation index of each pea germplasm, and comprehensively analyzes to obtain the comprehensive performance evaluation value of each pea germplasm. Pea germplasms with strong growth adaptability often have stronger disease resistance. This is because these germplasms can better adapt to the environment, and thus show stronger resistance when facing diseases. Therefore, pea varieties with a high growth adaptability evaluation index often also have a high disease resistance evaluation index; similarly, pea germplasms with strong growth adaptability also have stronger stress resistance. These germplasms can maintain a good growth state under adverse conditions such as drought, high temperature and low temperature, thus ensuring the yield and quality of peas. Therefore, there is also a positive correlation between the growth adaptability evaluation index and the stress resistance evaluation index; and pea germplasms with strong disease resistance often have stronger growth adaptability because these germplasms can better resist the invasion of diseases, thus maintaining a good growth state and yield; at the same time, pea germplasms with strong disease resistance also often show stronger stress resistance under adverse conditions because these germplasms have stronger physiological regulation ability and metabolic adaptability, and can maintain good growth and development under adverse conditions. Therefore, there is also a certain positive correlation between the disease resistance evaluation index and the stress resistance evaluation index; and pea germplasms with strong stress resistance often have stronger growth adaptability because these germplasms can maintain a good growth state under adverse conditions, thus ensuring the yield and quality of peas. Therefore, there is a positive correlation between the stress resistance evaluation index and the growth adaptability evaluation index; although the stress resistance evaluation index and the disease resistance evaluation index are two different evaluation indicators, there is also a certain association between them. This is because pea germplasms with strong stress resistance often have stronger physiological regulation ability and metabolic adaptability, and these characteristics also help to improve the disease resistance ability of peas. Through comprehensive analysis, a more comprehensive, accurate and in-depth comprehensive performance evaluation value of peas can be obtained.
[0082] It should be noted that in this embodiment, three key factors are considered, namely, the growth adaptability evaluation index of each pea germplasm, the pea disease resistance evaluation index, and the pea stress resistance evaluation index. Pea germplasms with strong adaptability, good disease resistance, and strong stress resistance can be screened out, so as to better cope with various environmental conditions and disease challenges, improve the yield and quality of peas. Moreover, high-quality pea germplasms can reduce the occurrence of pests and diseases, reduce the use of pesticides and fertilizers, further improve the planting efficiency, enhance the adaptability of the variety, help reduce the risks of agricultural production, boost farmers' confidence in planting, and promote the progress and innovation of pea breeding technology. It can help breeders more accurately select pea germplasms with excellent traits for hybridization and breeding, thereby improving the genetic level of pea varieties, contributing to the sustainable and healthy development of the pea industry, and injecting new vitality and impetus into agricultural production. By weighting the impacts of the growth adaptability evaluation index of each pea germplasm, the pea disease resistance evaluation index, and the pea stress resistance evaluation index, their relative importance in the evaluation index is reflected. The weights of different factors can be adjusted according to different needs, making the formula highly adaptable. It is not difficult to see that the greater the growth adaptability evaluation index, the pea disease resistance evaluation index, or the pea stress resistance evaluation index of each pea germplasm, the greater the comprehensive performance evaluation value of the peas of each pea germplasm. By evaluating the comprehensive performance evaluation value of the peas of each pea germplasm, potential pea germplasms can be quickly identified, thus shortening the breeding cycle, accelerating the promotion and application of new varieties, selecting pea germplasms with excellent quality, helping to meet the market demand for high-quality peas, promoting the upgrading and development of the pea industry, driving the innovation and development of pea breeding technology, providing technical support for the sustainable development of the pea industry, and at the same time providing a scientific basis for farmers' planting decisions, helping farmers formulate countermeasures in advance, reducing production risks, and ensuring the stability and sustainability of pea production.
[0083] It should be understood that in this embodiment, θ1, θ2, and θ3 are respectively the influencing factors of pea stress resistance evaluation corresponding to the preset growth adaptability evaluation index, pea disease resistance evaluation index, and pea stress resistance evaluation index in the pea germplasm database, representing the numerical values of the influence degrees of the growth adaptability evaluation index, pea disease resistance evaluation index, and pea stress resistance evaluation index on the pea stress resistance evaluation index. They can be directly obtained from the pea germplasm database during use, and their corresponding relationships can be pre-set mapping relationships. For example, the growth adaptability evaluation index, pea disease resistance evaluation index, and pea stress resistance evaluation index of each pea germplasm form a mapping set with the influencing factors of pea stress resistance evaluation corresponding to the preset growth adaptability evaluation index, pea disease resistance evaluation index, and pea stress resistance evaluation index in the pea germplasm database. Inputting the real-time growth adaptability evaluation index, pea disease resistance evaluation index, and pea stress resistance evaluation index into the mapping set to obtain the influencing factors of pea stress resistance evaluation corresponding to the growth adaptability evaluation index, pea disease resistance evaluation index, and pea stress resistance evaluation index. The mapping relationship therein can be one-to-one or many-to-one. In this example, its value range is between 0 and 1.
[0084] Specifically, the performance of peas is evaluated according to the comprehensive performance evaluation values of each pea germplasm. The specific evaluation process is as follows: Extract the comprehensive performance evaluation threshold of peas from the pea germplasm database, compare the comprehensive performance evaluation values of each pea germplasm with the comprehensive performance evaluation threshold of peas. If the comprehensive performance evaluation value of a certain pea germplasm is greater than or equal to the comprehensive performance evaluation threshold of peas, then evaluate this pea germplasm as qualified; if the comprehensive performance evaluation value of a certain pea germplasm is less than the comprehensive performance evaluation threshold of peas, then evaluate this pea germplasm as unqualified, and count the number of qualified pea germplasms.
[0085] If the number of qualified pea germplasms is zero, then re-cultivate the pea germplasm; if the number of qualified pea germplasms is not zero, then screen out the optimal pea germplasm.
[0086] It should be explained that in this embodiment, the optimal pea germplasm is screened out. The specific screening process is as follows: Sort the comprehensive performance evaluation values of each qualified pea germplasm in descending order, and mark the pea germplasm with the largest comprehensive performance evaluation value as the optimal pea germplasm.
[0087] Refer to Figure 2 As shown, the second aspect of the present invention provides a comprehensive disease and stress resistance cultivation method for high-altitude peas with concurrent rain and heat seasons, including: obtaining high-altitude peas with concurrent rain and heat seasons, marking them as each pea germplasm, and selecting sample plants from each pea germplasm for cultivation, and collecting the growth and development data of each sample plant of each pea germplasm.
[0088] The growth and development data of each sample plant of each pea germplasm are processed to obtain the growth adaptability evaluation index of each pea germplasm, and the growth cycle of each pea germplasm is matched according to the growth adaptability evaluation index of each pea germplasm.
[0089] Collect the cultivation data of each sample plant of each pea germplasm in the preset growth cycle, including disease resistance data and stress resistance data. Process the disease resistance data to obtain the pea disease resistance evaluation index of each pea germplasm, process the stress resistance data to obtain the pea stress resistance evaluation index of each pea germplasm, and comprehensively analyze the growth adaptability evaluation index, pea disease resistance evaluation index and pea stress resistance evaluation index of each pea germplasm to obtain the pea comprehensive performance evaluation value of each pea germplasm.
[0090] Evaluate the pea performance according to the pea comprehensive performance evaluation value of each pea germplasm, obtain the evaluation result and give feedback.
[0091] Pea germplasm database, used to store pea germplasm related data, including: critical average leaf area, critical plant height, critical number of branches, critical incidence rate, critical leaf withering rate, critical average disease resistance enzyme activity, critical germination rate, critical chlorophyll content, critical malondialdehyde content, influence factor of pea comprehensive performance evaluation corresponding to the set growth adaptability evaluation index, influence factor of pea comprehensive performance evaluation corresponding to the set pea disease resistance evaluation index, influence factor of pea comprehensive performance evaluation corresponding to the set pea stress resistance evaluation index, and pea comprehensive performance evaluation threshold and other indicators.
[0092] The above content is only an example and illustration of the structure of the present invention. Those skilled in the art of this technology make various modifications or supplements to the described specific embodiments or use similar methods to replace them. As long as they do not deviate from the structure of the invention or exceed the scope defined by the present invention, they should all belong to the protection scope of the present invention.
Claims
1. Comprehensive disease-resistant and stress-resistant cultivation system for pea germplasm with concurrent rain and heat in the plateau, characterized in that, Including: A pea data collection module, which is used to obtain plateau pea germplasms with concurrent rainfall and heat, label each pea germplasm, select sample plants from each pea germplasm for cultivation, and collect the growth and development data of each sample plant of each pea germplasm; A growth cycle matching module, which is used to process the growth and development data of each sample plant of each pea germplasm to obtain the growth adaptability evaluation index of each pea germplasm. The specific processing process is as follows: The growth and development data includes average leaf area, plant height, and number of branches; Extract the critical average leaf area, critical plant height, and critical number of branches from the pea germplasm database, and comprehensively analyze to obtain the growth adaptability evaluation index of each pea germplasm; Match the growth cycle of each pea germplasm according to the growth adaptability evaluation index of each pea germplasm, A comprehensive performance analysis module, which is used to collect the cultivation data of each sample plant of each pea germplasm in a preset growth cycle, including disease resistance data and stress resistance data, and process the disease resistance data to obtain the pea disease resistance evaluation index of each pea germplasm. The specific processing process is as follows: The disease resistance data includes incidence rate, leaf withering rate, and average disease resistance enzyme activity; Extract the critical incidence rate, critical leaf withering rate, and critical average disease resistance enzyme activity from the pea germplasm database, and comprehensively analyze to obtain the pea disease resistance evaluation index of each pea germplasm; Process the stress resistance data to obtain the pea stress resistance evaluation index of each pea germplasm. The specific processing process is as follows: The stress resistance data includes germination rate, chlorophyll content, and malondialdehyde content; Extract the critical germination rate, critical chlorophyll content, and critical malondialdehyde content from the pea germplasm database, and comprehensively analyze to obtain the pea stress resistance evaluation index of each pea germplasm; According to the growth adaptability evaluation index, pea disease resistance evaluation index, and pea stress resistance evaluation index of each pea germplasm, comprehensively analyze to obtain the pea comprehensive performance evaluation value of each pea germplasm A pea performance evaluation module, which is used to evaluate the pea performance according to the pea comprehensive performance evaluation value of each pea germplasm, obtain the evaluation result and give feedback. The specific evaluation process is as follows: Extract the pea comprehensive performance evaluation threshold from the pea germplasm database, compare the pea comprehensive performance evaluation value of each pea germplasm with the pea comprehensive performance evaluation threshold. If the pea comprehensive performance evaluation value of a certain pea germplasm is greater than or equal to the pea comprehensive performance evaluation threshold, then evaluate this pea germplasm as qualified. If the pea comprehensive performance evaluation value of a certain pea germplasm is less than the pea comprehensive performance evaluation threshold, then evaluate this pea germplasm as unqualified, and count the number of qualified pea germplasms; If the number of qualified pea germplasms is zero, then re-cultivate the pea germplasms. If the number of qualified pea germplasms is not zero, then screen out the optimal pea germplasms.
2. The comprehensive disease and stress resistance cultivation system for pea germplasm with concurrent rain and heat in the plateau according to claim 1, characterized in that: The matching of the growth cycle of each pea germplasm according to the growth adaptability evaluation index of each pea germplasm, the specific matching process is as follows: Input the growth adaptability evaluation index of each pea germplasm into the pea germplasm database to match the growth cycle of each pea germplasm corresponding to the growth adaptability evaluation index interval of each pea germplasm. The growth cycle is the required time for pea development to maturity.
3. The comprehensive disease and stress resistance cultivation system for pea germplasms with concurrent rain and heat in the plateau according to claim 1, characterized in that: The comprehensive analysis obtains the pea comprehensive performance evaluation values of each pea germplasm. The specific analysis process is as follows: Based on the growth adaptability evaluation index, pea disease resistance evaluation index, and pea stress resistance evaluation index of each pea germplasm, the pea comprehensive performance evaluation values of each pea germplasm are obtained through comprehensive analysis. The pea comprehensive performance evaluation values of each pea germplasm are used to quantify the comprehensive performance of each pea germplasm.
4. The comprehensive disease and stress resistance cultivation system for pea germplasm with concurrent rain and heat in the plateau according to claim 1, characterized in that: The process of screening to obtain the optimal pea germplasm is as follows: Sort the pea comprehensive performance evaluation values of each qualified pea germplasm in descending order, and mark the pea germplasm with the largest pea comprehensive performance evaluation value as the optimal pea germplasm.
5. The comprehensive disease and stress resistance cultivation system for pea germplasm with concurrent rainfall and heat in the plateau according to claim 1, characterized in that: The pea disease resistance evaluation index of each pea germplasm has the following specific numerical expression: Among them, represents the pea disease resistance evaluation index of the i-th pea germplasm, represents the incidence rate of the i-th pea germplasm, represents the critical incidence rate, and e represents the natural constant, represents the leaf withering rate of the j-th sample plant of the i-th pea germplasm, represents the critical leaf withering rate, represents the average disease resistance enzyme activity of the j-th sample plant of the i-th pea germplasm, represents the critical average disease resistance enzyme activity, represents the influence factor of pea disease resistance evaluation corresponding to the set incidence rate, represents the influence factor of pea disease resistance evaluation corresponding to the set leaf withering rate, represents the influence factor of pea disease resistance evaluation corresponding to the set average disease resistance enzyme activity. i represents the number of each pea germplasm, i = 1, 2, 3,..., m, where m represents the total number of pea germplasms, and j represents the number of each sample plant, j = 1, 2, 3,..., n, where n represents the total number of sample plants.
6. A method for an integrated disease and stress resistance cultivation system applied to the high-altitude pea germplasm with simultaneous rain and heat in the same season according to any one of claims 1-5, characterized in that: It includes: Obtain pea germplasms with rain and heat in the same season on the plateau, mark them as each pea germplasm, select sample plants from each pea germplasm for cultivation, and collect the growth and development data of each sample plant of each pea germplasm. Process the growth and development data of each sample plant of each pea germplasm to obtain the growth adaptability evaluation index of each pea germplasm, and match the growth cycle of each pea germplasm according to the growth adaptability evaluation index of each pea germplasm. Collect the cultivation data of each sample plant of each pea germplasm in the preset growth cycle, including disease resistance data and stress resistance data. Process the disease resistance data to obtain the pea disease resistance evaluation index of each pea germplasm, process the stress resistance data to obtain the pea stress resistance evaluation index of each pea germplasm, and comprehensively analyze the growth adaptability evaluation index, pea disease resistance evaluation index, and pea stress resistance evaluation index of each pea germplasm to obtain the pea comprehensive performance evaluation value of each pea germplasm. Evaluate the pea performance according to the pea comprehensive performance evaluation value of each pea germplasm, obtain the evaluation result and give feedback.
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