A whitening, moisturizing and anti-wrinkle emulsion mask and its preparation method and application

Abstract

The present invention relates to a kind of emulsion mask with whitening, moisturizing and anti-wrinkle effects and its preparation method and application. In particular, oriental orientalis leaf fermentation liquid and ginkgo leaf extract are selected as the main components of the mask liquid. These components are highly compatible with the skin, mild and non-irritating, and hypoallergenic. In addition, silk protein, vitamin C, niacinamide and inositol are also specially added to the mask. These components work together to effectively inhibit tyrosinase activity, reduce free radicals, block melanin production, thereby reducing spots, making the skin smooth and delicate, brightening the skin tone, and enhancing the whitening effect. The mask base cloth uses silk protein fiber as the core material, and is integrated with polyvinyl alcohol and chitosan, which not only helps to lift and tighten the skin, but also is beneficial to the load of the film liquid components. The mask not only has a significant moisturizing and hydrating effect, low irritation, but also can enhance skin elasticity, whiten the skin, resist aging, and effectively control oil secretion, meeting the market demand for multifunctional and high-performance masks.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to an emulsion mask with whitening, moisturizing and anti-wrinkle effects, and a preparation method and application thereof. Background Art

[0002] As a branch of skin care products, facial masks carry multiple functions of beauty care, such as hydrating, whitening, anti-aging, and regulating oil balance. Its mechanism of action is that when the mask covers the face, it can temporarily isolate the outside air and pollution, while raising the skin temperature, opening the pores, accelerating the secretion of sweat glands and the metabolic process, increasing the oxygen content of the skin, and helping the skin to excrete metabolic waste and oil. The moisture in the mask can penetrate into the surface of the skin, making the skin soft, thus making the skin look more shiny and elastic.

[0003] Moisturizing is an eternal theme in the field of skin care. It is essential for maintaining skin health and delaying aging. As we age, the skin's moisturizing mechanism will gradually be damaged, resulting in a decrease in the moisture in the skin tissue cells and between them. When the moisture content of the stratum corneum drops below 10%, the skin will become dry, lose elasticity, and wrinkle, thereby accelerating the aging process. In addition, the skin will be affected by external factors such as heat, cold, wind, detergents and ultraviolet radiation, making the skin surface drier. Therefore, keeping the skin hydrated and reducing water loss are essential for maintaining skin health. With the improvement of living standards, people's demand for skin care is increasing, especially for whitening and anti-wrinkle. This trend has promoted the popularity of various functional masks on the market, including moisturizing, anti-wrinkle, freckle removal, whitening and anti-aging. Although there are many types of masks on the market, their effects are often relatively single and the effects vary greatly, which makes it difficult for many products to meet consumers' expectations. In order to achieve their multi-faceted effects, the current mask products on the market often need to add a large amount of chemical ingredients such as moisturizers and antibacterial agents, which leads to mask products containing chemical additives that may cause allergic reactions during use and may even cause irreversible skin damage. Therefore, consumers have put forward higher requirements for the safety of skin care products. Currently, most facial masks on the market are mainly for moisturizing or anti-aging, with relatively single functions, or even if they have multiple functions, the functional effects are uneven and the comprehensive ability is not strong.

[0004] Ginkgo leaf extract is rich in a variety of bioactive ingredients, including flavonoids, terpene lactones, organic acids, organic alcohols, vitamins and amino acids. Among them, flavonoids are highly effective oxygen free radical scavengers that can protect skin cells from excessive oxidation damage, thereby prolonging the life of cells. In addition, flavonoids can also slow down the formation of peroxy lipids and enhance the skin's anti-aging ability. Alisma leaves are rich in protein, starch and triterpenoid compounds. The fermented Alisma leaves are richer in flavonoids and polyphenols. These ingredients can provide nutrition to the skin, eliminate excess free radicals in the body, make the skin smooth and delicate, reduce wrinkles, and improve the overall appearance of the skin. The present invention provides a new facial mask and a preparation method thereof, which aims to solve the problem that existing facial mask products have a single function or insufficient multiple functions. We innovatively use ginkgo leaf extract and Alisma leaf fermentation liquid as core ingredients to develop a facial mask with simple ingredients and comprehensive functions. This mask not only has excellent moisturizing and hydrating capabilities, but is also low in irritation. It can also whiten the skin, enhance skin elasticity, resist aging, and effectively control oil secretion, meeting the market demand for multifunctional, high-performance masks. Summary of the invention

[0005] The purpose of the present invention is to overcome the defects of the above-mentioned prior art and to provide a whitening, moisturizing and anti-wrinkle emulsion mask and its preparation method and application. The mask can not only deeply hydrate and moisturize, but also effectively reduce the deposition of melanin and whiten the skin. At the same time, it has the effect of significantly resisting aging and effectively controlling oil secretion.

[0006] In order to achieve the above-mentioned purpose of the present invention, the following technical scheme is adopted:

[0007] The invention provides an emulsion mask with whitening, moisturizing and anti-wrinkle effects, comprising a membrane liquid and a silk protein fiber-based cloth. The membrane liquid comprises, by weight, 0.5-2 parts of silk protein, 0.3-1.2 parts of vitamin C, 1-3 parts of inositol, 0.1-0.8 parts of oriental water rhizome fermentation liquid, 0.5-2 parts of ginkgo leaf extract, 0.5-1.5 parts of bird's nest acid, 0.5-2.5 parts of niacinamide, 1-5 parts of auxiliary ingredients and 80-120 parts of water.

[0008] The silk protein fiber base fabric comprises, by weight, 20-40 parts of silk protein peptides, 5-10 parts of polyvinyl alcohol, and 8-15 parts of chitosan, and the silk protein fiber mask base fabric is prepared by electrostatic spinning.

[0009] The preparation method of the oriental oriental leaf fermented liquid comprises the following steps:

[0010] S11: freeze-dry and grind fresh oriental oriental leaves into oriental ...

[0011] S12: sterilize and cool the mixed solution prepared in S11, inoculate probiotics, and then ferment and culture. After centrifugation, take the supernatant to obtain the fermented liquid of Alisma orientalis leaves; wherein the probiotics are Aspergillus niger and Lactobacillus plantarum mixed in a volume ratio of 1:3-6, and the effective live bacteria count of Aspergillus niger and Lactobacillus plantarum is 1×10 8 -8×10 8 CFU / mL.

[0012] The fermentation culture conditions are as follows: the inoculation amount of probiotics relative to the mixed solution is 0.5-15 vol%, the temperature is 30-45° C., the initial pH of the mixed solution is 5-7, and the fermentation time is 12-48 hours.

[0013] The Aspergillus niger and Lactobacillus plantarum of the present invention are obtained by inoculating the corresponding bacterial powder into the culture medium for fermentation. There are no particularly strict requirements for the culture medium used. The key is to ensure that the number of effective live bacteria of Aspergillus niger and Lactobacillus plantarum reaches 1×10 8 -8×10 8 The purpose of the present invention can be achieved in the range of CFU / mL.

[0014] The ginkgo leaf extract used in the present invention is prepared as follows:

[0015] After selection, obtain ginkgo leaves with intact leaves and normal color, freeze-dry the ginkgo leaves, grind them to obtain ginkgo leaf powder, set aside, weigh the ginkgo leaf powder and add it to 50% ethanol solution at a mass ratio of 1:30-50, stir and soak it, extract it with ultrasonic vibration for 1-3h, extract it twice, centrifuge it, collect and combine the supernatant, remove the solvent, and obtain ginkgo leaf extract.

[0016] Furthermore, the present invention relates to a method for preparing a whitening, moisturizing and anti-wrinkle emulsion mask, comprising the following preparation steps:

[0017] 1) Preparation of mask solution: weigh 0.5-2 parts of silk protein, 0.3-1.2 parts of vitamin C, 1-3 parts of inositol, 0.1-0.8 parts of oriental leaf fermentation liquid, 0.5-2 parts of ginkgo leaf extract, 0.5-1.5 parts of bird's nest acid, 0.5-2.5 parts of niacinamide, 1-5 parts of auxiliary ingredients and 80-120 parts of water, stir and mix evenly at 40-60° C. to obtain mask solution;

[0018] 2) Silk protein fiber base fabric, using electrospinning to prepare the silk protein fiber mask base fabric;

[0019] 3) Preparation of a whitening, moisturizing and anti-wrinkle emulsion mask: soak the base fabric in a membrane solution 40-60 times its mass at 15-30° C. for 30-60 minutes to prepare a whitening, moisturizing and anti-wrinkle emulsion mask.

[0020] Further, the preparation of the base fabric comprises the following steps:

[0021] S21: weighing 20-40 parts of silk protein peptide, 5-10 parts of polyvinyl alcohol, and 8-15 parts of chitosan, dissolving the polyvinyl alcohol in deionized water to prepare a polyvinyl alcohol solution with a mass fraction of 10%-15%; then adding the silk protein peptide and chitosan to the prepared polyvinyl alcohol solution to obtain a mixed solution;

[0022] S22: stirring the mixed solution obtained in step S21 at a constant temperature until a uniform solution is formed, and then standing to degas for standby use to obtain a spinning solution;

[0023] S23: electrospinning the spinning solution, and obtaining a mask base fabric containing silk protein fibers after drying the product.

[0024] Furthermore, the auxiliary components include, by weight, 0.10-1.00 parts of thickener, 0.02-0.10 parts of chelating agent, 0.2-10 parts of polyol, and 0.5-2 parts of pH regulator.

[0025] The thickener is at least one of sodium polyacrylate, carboxymethyl cellulose, hydroxyethyl cellulose and xanthan gum.

[0026] The chelating agent is selected from at least one of disodium ethylenediaminetetraacetate, trisodium ethylenediaminetetraacetate, tetrasodium ethylenediaminetetraacetate, ethylenediaminetetraacetic acid and trisodium ethylenediamine disuccinate.

[0027] The polyol is one or more of glycerol, butylene glycol and propylene glycol.

[0028] The pH regulator is selected from at least one of aminomethyl propanol, arginine, citric acid and sodium citrate.

[0029] The present invention also relates to the application of the whitening, moisturizing and anti-wrinkle facial mask, which is characterized in that it is used for moisturizing, whitening and anti-wrinkle of the skin.

[0030] The sericin used in the present invention contains multiple amino acids with long side chains and polar hydrophilic groups on its molecular chain. These groups are mainly distributed on the surface of the polypeptide chain, which can transfer moisture from the inside of the product to the stratum corneum of the skin and combine with it to directly promote the skin's absorption of moisture. In addition, sericin can also effectively inhibit the activity of tyrosinase, reduce the production of melanin in the skin, prevent ultraviolet damage to the skin, and reduce the formation of spots. Therefore, adding sericin to the mask liquid can significantly improve the whitening, moisturizing and anti-aging effects of the product. At the same time, silk protein is rich in amino acids and trace elements, has good affinity with human skin, and is easily absorbed by the skin. Its excellent biocompatibility also makes silk protein suitable for cell attachment and growth, further enhancing its affinity with human skin.

[0031] The facial mask liquid of the present invention uses the fermented liquor of Alisma orientalis leaves, which is rich in flavonoids, polyphenols and triterpenoid compounds, and exhibits a strong scavenging ability for DPPH free radicals and hydroxyl free radicals. In addition, the inhibitory effect of this fermented liquor on tyrosinase is particularly significant, which helps to reduce the production of melanin in the skin, so it not only has a whitening effect, but also provides antioxidant protection. The present invention particularly screens Aspergillus niger and Lactobacillus plantarum as composite fermentation bacteria, and the two act synergistically, and the effect is better. Among them, Aspergillus niger can efficiently secrete a variety of active enzymes such as cellulase and pectinase, which help to promote the dissolution of active ingredients such as flavonoids and polyphenols in Alisma orientalis leaves; while Lactobacillus plantarum can promote the dissolution of original polyphenols and polysaccharides, and increase the content of polyphenols through biotransformation. This synergistic effect further enhances the efficacy of the facial mask in resisting aging and effectively controlling oil secretion.

[0032] Ginkgo biloba extract has powerful antioxidant properties. It can effectively inhibit the activity of collagenase and elastase, prevent the reduction or denaturation of elastin content, thereby maintaining the elasticity of the skin and achieving anti-wrinkle effects. In addition, Ginkgo biloba extract also helps whiten the skin and improve the overall health and radiance of the skin.

[0033] The present invention particularly selects the fermented liquor of oriental orientalis leaves and the extract of ginkgo biloba leaves, which are all pure natural ingredients. They are not only extremely compatible with human skin, mild and non-irritating to the skin, and hypoallergenic, but also can synergistically enhance the moisturizing, whitening and anti-aging effects. Vitamin C, nicotinamide and inositol are also particularly added, and these ingredients act together to inhibit the activity of tyrosinase and reduce the generation of melanin from the source. They can also remove free radicals, block the conversion of tyrosine to melanin through antioxidant action, and promote the decomposition of melanin in the stratum corneum, help even out skin color, make the skin smoother and more delicate, brighten the skin color, enhance the skin whitening effect, and improve the anti-aging ability of the skin.

[0034] Furthermore, the present invention uses silk protein fiber as the core material of the mask base fabric. This silk protein fiber is not only rich in silk protein, but also extremely friendly to the skin. After being absorbed by the skin, the amino acids in the silk protein can be converted into nutrients required by the skin, promoting the production of collagen, thereby making the skin more delicate, smooth, shiny and elastic. In order to enhance the effect, polyvinyl alcohol and chitosan are also specially incorporated into the mask cloth. The amino and hydroxyl groups on the surface of chitosan, as well as the hydroxyl groups on the surface of polyvinyl alcohol, form strong hydrogen bonds with the hydroxyl groups in the silk protein fiber. This interaction not only helps to lift and tighten the skin, but also significantly improves the comprehensive efficacy of the mask's moisturizing and oil-resistant properties.

[0035] Compared with the prior art, the beneficial effects of the present invention are:

[0036] 1) The mask of the present invention uses fermented broth of oriental orientalis leaves and extract of ginkgo biloba leaves as plant extracts, which are mild and non-irritating to the skin. The mask also contains phytosphingolipids, vitamin C, niacinamide, inositol and swan acid, which can inhibit the activity of tyrosinase from the source, thereby effectively improving the moisturizing, whitening and anti-aging effects of the mask.

[0037] 2) The bird's nest acid used in the mask of the present invention has a strong penetration ability, which can easily penetrate the skin barrier, directly reach the basal layer of the skin, activate cells, effectively repair and strengthen the skin barrier, lock in moisture, tighten loose skin, and make the skin smoother and more elastic; at the same time, inositol, as a highly efficient moisturizing ingredient, can absorb and retain moisture in the air and promote the penetration of active substances into the deep layers of the skin. Combining bird's nest acid with inositol not only helps inositol penetrate into the basal layer of the skin, but also synergistically enhances the moisturizing effect of the mask and improves the elasticity of the skin.

[0038] 3) The mask base fabric of the present invention uses silk protein fiber as the core material, and polyvinyl alcohol and chitosan are specially added. These ingredients all show excellent biocompatibility and fiber-forming and film-forming properties, are mild and non-irritating to human skin, and do not cause cytotoxicity. Electrospinning technology can be used to produce nanofiber mask base fabrics containing silk protein, which has better skin fit than traditional base fabrics. The amino and hydroxyl groups on the surface of chitosan, as well as the hydroxyl groups on the surface of polyvinyl alcohol, form strong hydrogen bonds with the hydroxyl groups in the silk protein fibers. This interaction not only helps to lift and tighten the skin, but also significantly enhances the moisturizing, whitening and anti-aging abilities of the mask, especially for improving skin elasticity and reducing skin oil content. DETAILED DESCRIPTION

[0039] The technical solution of the present invention is further described below by specific implementation methods. However, the following examples are only simplified examples of the present invention and do not represent or limit the scope of protection of the present invention. The scope of protection of the present invention shall be subject to the claims.

[0040] Experimental Materials:

[0041] The bird's nest acid used in the present invention was purchased from Xi'an Green Peptide Biotechnology Co., Ltd.

[0042] The nicotinamide used in the present invention was purchased from Shanghai Tongtian Biotechnology Co., Ltd.

[0043] The inositol used in the present invention was purchased from Guangzhou Hengtao Trading Co., Ltd.

[0044] The Aspergillus niger described in the present invention was purchased from China General Microorganism Collection Center, with a collection number of CGMCC No.3.15663.

[0045] The Lactobacillus plantarum described in the present invention is purchased from China Center for Type Culture Collection, with a collection number of CCTCC NO: M2015126.

[0046] Unless otherwise specified, a component (such as a thickener or a chelating agent) in the parallel embodiments and comparative examples of the present invention is the same commercially available product. The amount of the components in the following examples is 1 g per weight part or per portion unless otherwise specified.

[0047] Example 1

[0048] A whitening, moisturizing and anti-wrinkle emulsion mask comprises a membrane liquid and a silk protein fiber base cloth, wherein the membrane liquid comprises, by weight, 0.5 parts of silk protein, 0.5 parts of vitamin C, 1 part of inositol, 0.2 parts of orientalis leaf fermentation liquid, 1 part of ginkgo leaf extract, 0.5 parts of bird's nest acid, 1.5 parts of niacinamide, 3 parts of auxiliary ingredients and 100 parts of water, wherein the auxiliary ingredients are 0.2 parts of sodium polyacrylate, 0.8 parts of disodium ethylenediaminetetraacetic acid, 1.5 parts of glycerol and 0.5 parts of citric acid.

[0049] The silk protein fiber base fabric comprises, by weight, 40 parts of silk protein peptides, 10 parts of polyvinyl alcohol, and 15 parts of chitosan, and the silk protein fiber mask base fabric is prepared by electrostatic spinning.

[0050] The preparation method of the oriental oriental leaf fermented liquid comprises the following steps:

[0051] S11: freeze-dry and grind fresh oriental oriental leaves into oriental ...

[0052] S12: sterilize and cool the mixed solution prepared in S11, inoculate probiotics, and then ferment and culture, and take the supernatant after centrifugation to obtain the fermented solution of Alisma orientalis leaves. The inoculation amount of probiotics relative to the mixed solution is 5 vol%, the fermentation temperature is 35°C, the initial pH of the mixed solution is 6, and the fermentation time is 36 hours.

[0053] The probiotics are a mixture of Aspergillus niger and Lactobacillus plantarum in a volume ratio of 1:4, wherein the number of effective live bacteria in the Aspergillus niger solution is 1×10 8 CFU / mL; the number of effective live bacteria in Lactobacillus plantarum liquid is 8×10 8 CFU / mL.

[0054] The ginkgo leaf extract used in this example was prepared as follows:

[0055] Ginkgo leaves with intact leaves and normal color were selected, freeze-dried and crushed to obtain ginkgo leaf powder for later use. The ginkgo leaf powder was weighed and added to 50% ethanol solution in a mass ratio of 1:50, stirred and soaked, and extracted with ultrasonic vibration for 2 hours. The extraction was repeated twice, and the supernatant was collected and combined, and the solvent was removed to obtain ginkgo leaf extract.

[0056] The present embodiment relates to a method for preparing a whitening, moisturizing and anti-wrinkle emulsion mask, comprising the following preparation steps:

[0057] 1) Preparation of mask solution: weigh 0.5 parts of silk protein, 0.5 parts of vitamin C, 1 part of inositol, 0.2 parts of oriental leaf fermentation liquid, 1 part of ginkgo leaf extract, 0.5 parts of bird's nest acid, 1.5 parts of niacinamide, 3 parts of auxiliary ingredients and 100 parts of water, stir and mix evenly at 50°C to obtain a mask solution.

[0058] 2) Silk protein fiber base fabric, specifically comprising the following steps:

[0059] S21: Weigh 40 parts of silk protein peptide, 10 parts of polyvinyl alcohol, and 15 parts of chitosan, dissolve the polyvinyl alcohol in deionized water, and prepare a polyvinyl alcohol solution with a mass fraction of 15%; then add the silk protein peptide and chitosan to the prepared polyvinyl alcohol solution to obtain a mixed solution;

[0060] S22: stirring the mixed solution obtained in step S21 at a constant temperature until a uniform solution is formed, and then standing to degas for standby use to obtain a spinning solution;

[0061] S23: electrospinning the spinning solution, and obtaining a mask base fabric containing silk protein fibers after drying the product.

[0062] 3) Preparation of a whitening, moisturizing and anti-wrinkle emulsion mask: The base fabric of step 2 was immersed in a membrane solution of step 1) with a mass 60 times that of the base fabric at 15° C. for 60 minutes to prepare a whitening, moisturizing and anti-wrinkle emulsion mask.

[0063] Example 2

[0064] A whitening, moisturizing and anti-wrinkle emulsion mask comprises a membrane liquid and a silk protein fiber base cloth, wherein the membrane liquid comprises, by weight, 1 part of silk protein, 1.2 parts of vitamin C, 3 parts of inositol, 0.6 parts of orientalis leaf fermentation liquid, 2 parts of ginkgo leaf extract, 1.5 parts of bird's nest acid, 2 parts of niacinamide, 3 parts of auxiliary ingredients and 120 parts of water, wherein the auxiliary ingredients are 0.5 parts of carboxymethyl cellulose, 0.8 parts of tetrasodium ethylenediaminetetraacetic acid, 1.2 parts of propylene glycol and 0.5 parts of arginine.

[0065] The silk protein fiber base fabric comprises, by weight, 30 parts of silk protein peptides, 5 parts of polyvinyl alcohol, and 8 parts of chitosan, and the silk protein fiber mask base fabric is prepared by electrostatic spinning.

[0066] The preparation method of the oriental oriental leaf fermented liquid comprises the following steps:

[0067] S11: freeze-dry fresh oriental oriental leaves and grind them into oriental ...

[0068] S12: sterilize and cool the mixed solution prepared in S11, inoculate probiotics, and then ferment and culture, and take the supernatant after centrifugation to obtain the fermented solution of Alisma orientalis leaves. The inoculation amount of probiotics relative to the mixed solution is 10 vol%, the temperature is 30°C, the initial pH of the mixed solution is 5, and the fermentation time is 12 hours.

[0069] The probiotics are a mixture of Aspergillus niger and Lactobacillus plantarum in a volume ratio of 1:6, wherein the number of effective live bacteria in the Aspergillus niger solution is 5×10 8 CFU / mL; the number of effective live bacteria in Lactobacillus plantarum liquid is 2×10 8 CFU / mL.

[0070] The ginkgo leaf extract used in this example was prepared as follows:

[0071] After selection, obtain ginkgo leaves with intact leaves and normal color, freeze-dry the ginkgo leaves, grind them to obtain ginkgo leaf powder, set aside, weigh the ginkgo leaf powder, add it into 50% ethanol solution at a mass ratio of 1:30, stir and soak, extract with ultrasonic vibration for 3 hours, extract twice, centrifuge, collect and combine the supernatant, remove the solvent, and obtain ginkgo leaf extract.

[0072] The present embodiment relates to a method for preparing a whitening, moisturizing and anti-wrinkle emulsion mask, comprising the following preparation steps:

[0073] 1) Preparation of mask solution: weigh 1 part of silk protein, 1.2 parts of vitamin C, 3 parts of inositol, 0.6 parts of oriental leaf fermentation liquid, 2 parts of ginkgo leaf extract, 1.5 parts of bird's nest acid, 2 parts of niacinamide, 3 parts of auxiliary ingredients and 120 parts of water, stir and mix evenly at 40°C to obtain mask solution;

[0074] 2) Silk protein fiber base fabric, specifically comprising the following steps:

[0075] S21: weighing 30 parts of silk protein peptide, 5 parts of polyvinyl alcohol, and 8 parts of chitosan, dissolving the polyvinyl alcohol in deionized water to prepare a polyvinyl alcohol solution with a mass fraction of 10%; then adding the silk protein peptide and chitosan to the prepared polyvinyl alcohol solution to obtain a mixed solution;

[0076] S22: stirring the mixed solution obtained in step S21 at a constant temperature until a uniform solution is formed, and then standing to degas for standby use to obtain a spinning solution;

[0077] S23: electrospinning the spinning solution, and obtaining a mask base fabric containing silk protein fibers after drying the product.

[0078] 3) Preparation of a whitening, moisturizing and anti-wrinkle emulsion mask: The base fabric of step 2 was immersed in the membrane solution of step 1) with a mass 40 times that of the base fabric at 30° C. for 30 min to prepare a whitening, moisturizing and anti-wrinkle emulsion mask.

[0079] Example 3

[0080] A whitening, moisturizing and anti-wrinkle emulsion mask comprises a membrane liquid and a silk protein fiber-based cloth, wherein the membrane liquid comprises, by weight, 2 parts of silk protein, 0.3 parts of vitamin C, 2.5 parts of inositol, 0.2 parts of orientalis leaf fermentation liquid, 1 part of ginkgo leaf extract, 1 part of bird's nest acid, 2.5 parts of niacinamide, 2.5 parts of auxiliary components and 90 parts of water, wherein the auxiliary components are 0.4 parts of xanthan gum, 0.8 parts of ethylenediaminetetraacetic acid, 1 part of butylene glycol and 0.3 parts of aminomethyl propanol.

[0081] The silk protein fiber base fabric comprises, by weight, 20 parts of silk protein peptides, 10 parts of polyvinyl alcohol, and 15 parts of chitosan, and the silk protein fiber mask base fabric is prepared by electrostatic spinning.

[0082] The preparation method of the oriental oriental leaf fermented liquid comprises the following steps:

[0083] S11: freeze-dry and grind fresh oriental oriental leaves into oriental ...

[0084] S12: sterilize and cool the mixed solution prepared in S11, inoculate probiotics, and then ferment and culture, and take the supernatant after centrifugation to obtain the fermented solution of Alisma orientalis leaves. The inoculation amount of probiotics relative to the mixed solution is 15 vol%, the fermentation temperature is 35°C, the initial pH of the mixed solution is 6, and the fermentation time is 30 hours.

[0085] The probiotics are a mixture of Aspergillus niger and Lactobacillus plantarum in a volume ratio of 1:5, wherein the effective viable bacteria count in the Aspergillus niger solution is 7×10 8 CFU / mL; the number of effective live bacteria in Lactobacillus plantarum liquid is 3×10 8 CFU / mL.

[0086] The ginkgo leaf extract used in this example was prepared as follows:

[0087] Ginkgo leaves with intact leaves and normal color were selected, freeze-dried and crushed to obtain ginkgo leaf powder for later use. The ginkgo leaf powder was weighed and added to 50% ethanol solution in a mass ratio of 1:45, stirred and soaked, and extracted with ultrasonic vibration for 3 hours. The extraction was performed twice, centrifuged, the supernatant was collected and combined, and the solvent was removed to obtain ginkgo leaf extract.

[0088] The present embodiment relates to a method for preparing a whitening, moisturizing and anti-wrinkle emulsion mask, comprising the following preparation steps:

[0089] 1) Preparation of mask solution: weigh 2 parts of silk protein, 0.3 parts of vitamin C, 2.5 parts of inositol, 0.2 parts of oriental leaf fermentation liquid, 1 part of ginkgo leaf extract, 1 part of sialic acid, 2.5 parts of niacinamide, 2.5 parts of auxiliary ingredients and 90 parts of water, stir and mix evenly at 60°C to obtain mask solution.

[0090] 2) Silk protein fiber base fabric, specifically comprising the following steps:

[0091] S21: weighing 20 parts of silk protein peptide, 10 parts of polyvinyl alcohol, and 15 parts of chitosan, dissolving the polyvinyl alcohol in deionized water to prepare a polyvinyl alcohol solution with a mass fraction of 12%; then adding the silk protein peptide and chitosan to the prepared polyvinyl alcohol solution to obtain a mixed solution;

[0092] S22: stirring the mixed solution obtained in step S21 at a constant temperature until a uniform solution is formed, and then standing to degas for standby use to obtain a spinning solution;

[0093] S23: electrospinning the spinning solution, and obtaining a mask base fabric containing silk protein fibers after drying the product.

[0094] 3) Preparation of a whitening, moisturizing and anti-wrinkle emulsion mask: The base fabric of step 2 was immersed in the membrane solution of step 1) with a weight 50 times that of the base fabric at 20° C. for 50 minutes to prepare a whitening, moisturizing and anti-wrinkle emulsion mask.

[0095] Example 4

[0096] A whitening, moisturizing and anti-wrinkle emulsion mask comprises a membrane liquid and a silk protein fiber base cloth, wherein the membrane liquid comprises, by weight, 1.5 parts of silk protein, 0.8 parts of vitamin C, 1.5 parts of inositol, 0.4 parts of orientalis leaf fermentation liquid, 0.7 parts of ginkgo leaf extract, 0.8 parts of bird's nest acid, 2 parts of niacinamide, 2.5 parts of auxiliary ingredients and 100 parts of water, wherein the auxiliary ingredients are 0.4 parts of xanthan gum, 0.8 parts of ethylenediaminetetraacetic acid, 1 part of butylene glycol and 0.3 parts of aminomethyl propanol.

[0097] The silk protein fiber base fabric comprises, by weight, 25 parts of silk protein peptides, 8 parts of polyvinyl alcohol, and 12 parts of chitosan, and the silk protein fiber mask base fabric is prepared by electrostatic spinning.

[0098] The preparation method of the oriental oriental leaf fermented liquid comprises the following steps:

[0099] S11: freeze-dry and grind fresh oriental oriental leaves into oriental ...

[0100] S12: sterilize and cool the mixed solution prepared in S11, inoculate probiotics, and then ferment and culture, and take the supernatant after centrifugation to obtain the fermented solution of Alisma orientalis leaves. The inoculation amount of probiotics relative to the mixed solution is 15 vol%, the fermentation temperature is 40°C, the initial pH of the mixed solution is 6, and the fermentation time is 30 hours.

[0101] The probiotics are a mixture of Aspergillus niger and Lactobacillus plantarum in a volume ratio of 1:3, wherein the number of effective live bacteria in the Aspergillus niger solution is 6×10 8 CFU / mL; effective viable bacteria count in Lactobacillus plantarum solution 6×10 8 CFU / mL.

[0102] Other conditions are the same as in Example 1.

[0103] Example 5

[0104] A whitening, moisturizing and anti-wrinkle emulsion mask comprises a membrane liquid and a silk protein fiber base cloth, wherein the membrane liquid comprises, by weight, 0.8 parts of silk protein, 0.6 parts of vitamin C, 1.2 parts of inositol, 0.7 parts of orientalis leaf fermentation liquid, 1.5 parts of ginkgo leaf extract, 1 part of bird's nest acid, 2 parts of niacinamide, 2 parts of auxiliary ingredients and 110 parts of water, wherein the auxiliary ingredients are 0.3 parts of xanthan gum, 0.3 parts of ethylenediaminetetraacetic acid, 1 part of butylene glycol and 0.4 parts of aminomethyl propanol.

[0105] The silk protein fiber base fabric comprises, by weight, 35 parts of silk protein peptides, 10 parts of polyvinyl alcohol, and 8 parts of chitosan, and the silk protein fiber mask base fabric is prepared by electrostatic spinning.

[0106] In the preparation process of the fermented liquor of oriental oriental leaves, the probiotics are a mixture of Aspergillus niger and Lactobacillus plantarum in a volume ratio of 1:5.5, wherein the number of effective live bacteria in the Aspergillus niger liquor is 3×10 8 CFU / mL; effective viable bacteria count in Lactobacillus plantarum solution 7×10 8 CFU / mL.

[0107] Other conditions are the same as in Example 1.

[0108] Comparative Example 1

[0109] The difference from Example 1 is that: in the preparation process of the fermented liquor of oriental orientalis leaves, no Aspergillus niger was added, and Lactobacillus plantarum with the same volume and effective viable count was used instead of Aspergillus niger.

[0110] Comparative Example 2

[0111] The difference from Example 1 is that Lactobacillus plantarum was not added during the preparation of the fermented liquor of Alisma orientalis leaves, and Aspergillus niger with the same volume and effective viable count was used instead of Lactobacillus plantarum.

[0112] Comparative Example 3

[0113] The difference from Example 1 is that in the preparation process of the fermented liquor of oriental oriental leaves, the probiotics are Bacillus subtilis, and the effective viable bacteria count in the Bacillus subtilis liquor is 8×10 8 CFU / mL.

[0114] Comparative Example 4

[0115] The difference from Example 1 is that: Alisma orientalis leaf extract is used instead of Alisma orientalis leaf fermentation product. The preparation method of Alisma orientalis leaf extract is as follows: fresh Alisma orientalis leaves are freeze-dried and crushed into Alisma orientalis leaf powder, the Alisma orientalis leaf powder is weighed and added to 50% ethanol solution in a mass ratio of 1:30, and stirred and soaked, ultrasonically extracted for 3 hours, extracted twice, centrifuged, the supernatant was collected and combined, and the solvent was removed to obtain the Alisma orientalis leaf extract.

[0116] Comparative Example 5

[0117] The difference from Example 1 is that no silk protein is added, and an equal amount of water is used instead of silk protein.

[0118] Comparative Example 6

[0119] The difference from Example 1 is that no oriental oriental leaf fermentation liquid is added, and an equal amount of ginkgo leaf extract is used instead of oriental oriental leaf fermentation liquid.

[0120] Comparative Example 7

[0121] The difference from Example 1 is that no ginkgo leaf extract is added, and an equal amount of oriental oriental leaf fermentation liquid is used instead of the ginkgo leaf extract.

[0122] Comparative Example 8

[0123] The difference from Example 2 is that no silk protein peptide is added in the process of preparing the silk protein fiber-based cloth, and an equal amount of polyvinyl alcohol is used instead of silk protein peptide.

[0124] Comparative Example 9

[0125] The difference from Example 2 is that no polyvinyl alcohol is added in the process of preparing the silk protein fiber-based cloth, and an equal amount of silk protein peptide alcohol is used instead of polyvinyl alcohol.

[0126] Comparative Example 10

[0127] The difference from Example 2 is that chitosan is not added in the process of preparing the silk protein fiber-based cloth, and an equal amount of silk protein peptide alcohol is used instead of chitosan.

[0128] Performance Testing

[0129] The whitening, moisturizing and anti-wrinkle emulsion masks prepared in Examples 1-5 and Comparative Examples 1-10 were subjected to relevant performance tests, mainly including stimulation performance, moisturizing performance, whitening performance, anti-aging performance, skin elasticity and oil resistance, and the effects were evaluated as follows:

[0130] 1. Anti-irritation performance test

[0131] Healthy volunteers were selected, with an average of 15 groups, each group of 5 male and female volunteers. Each group of volunteers used the facial masks of Examples 1-5 and Comparative Examples 1-10 for 10 days, once in the morning and once in the evening; on the 11th day of using the facial masks, the irritation of the facial masks was tested for each user.

[0132] After the anti-irritation performance test, all volunteers participating in the test did not experience discomfort or allergic symptoms. Therefore, the facial mask of the present invention is confirmed to be mild and non-irritating, and will not cause allergic reactions on the human face. The moisturizing, whitening and anti-aging facial mask prepared by the present invention will not cause any discomfort or allergic symptoms.

[0133] 2. Determination of moisturizing effect

[0134] Healthy volunteers aged 30 to 45 years old, who were not allergic to cosmetics, had no acute facial inflammation, did not use hormone drugs or immunosuppressants, did not participate in other clinical trials, and did not apply external preparations, were randomly divided into 15 groups, 10 people in each group, and the masks provided by the above embodiments 1-5 and comparative examples 1-10 were used respectively. The facial skin moisture content was detected by using a skin moisture tester (Corneometer CM825) at 0min, 30min, 60min, 90min, and 120min, and the moisture content of the skin before and after using the mask was evaluated. The specific test results are shown in Table 1 below. The moisture content and the moisturizing rate after 2 hours of use are the average values ​​of each group of subjects. Moisturizing rate after 2h = moisture content at 120min / moisture content at 0min*100%.

[0135] Table 1 Moisturizing performance test

[0136]

[0137] According to the test results, the facial mask of the present invention exhibits excellent long-term moisturizing ability, and the moisturizing rate can still be maintained at more than 74% after 2 hours. In contrast, the facial masks of Comparative Examples 1-3, which only use the Alisma leaf membrane liquid fermented by Aspergillus niger, Lactobacillus plantarum or Bacillus subtilis, and the conventional Alisma leaf alcohol extract used in Comparative Example 4, have moisturizing properties similar to those of Examples 1-5 in the first 60 minutes, but their moisturizing properties significantly decrease after 60 minutes, showing a lack of long-term moisturizing ability. This phenomenon may be because the synergistic fermentation of Aspergillus niger and Lactobacillus plantarum increases the content of flavonoids and polyphenols in the Alisma leaf fermentation liquid, thereby enhancing the moisturizing effect. The facial masks of Comparative Examples 6-7 only use one of the Alisma leaf fermentation liquid or the Ginkgo leaf fermentation liquid, and their long-term moisturizing performance is significantly reduced, indicating that these two ingredients have a synergistic moisturizing effect. However, the mask base fabrics of Comparative Examples 8-10 lack one of the components of silk protein fiber, polyvinyl alcohol and chitosan, which weakens the hydrogen bonding effect between molecules and is not conducive to moisture retention, so their long-term moisturizing performance is also reduced.

[0138] 3. Whitening performance test

[0139] This study selected volunteers aged between 30 and 50 with whitening needs as subjects, and evenly distributed them into 15 different groups, with 10 people in each group. Each group used the mask products prepared by Examples 1-5 and Comparative Examples 1-10 respectively. During the three-week trial, the volunteers used the mask 6 times a week, only once a day, in the morning or evening, and 3 times each in the morning and evening. The skin red-melanin tester Hexameter MX18 was used to evaluate the changes in skin melanin content before and after the subjects used the mask composition. The measurement range of the instrument used is 0-999. The higher the measurement value, the higher the melanin content in the skin. The statistical results of the average content and change rate of melanin in the facial skin of the subjects before and after using the mask composition prepared by the present invention are shown in Table 2; wherein the average melanin content before use is recorded as M0, the average melanin content after one week of use is recorded as M1, and the average melanin content after three weeks of use is recorded as M2; the melanin change rate after one week of use is N1=100%*(M0-M1) / M0; the melanin change rate after three weeks of use is N2=100%*(M0-M2) / M0.

[0140] Table 2 Whitening performance test

[0141] Test samples M0 M1 M2 N1 N2 Example 1 264.2 236.3 193.8 10.56 26.65 Example 2 263.9 235.8 190.2 10.65 27.93 Example 3 264.1 231.4 191.4 12.38 27.53 Example 4 264.3 233.8 192.2 11.54 27.28 Example 5 263.8 234.7 190.6 11.03 27.75 Comparative Example 1 265.1 247.3 230.1 6.71 13.20 Comparative Example 2 264.8 246.9 231.5 6.76 12.58 Comparative Example 3 263.8 248.1 230.8 5.95 12.51 Comparative Example 4 265.1 247.8 232.4 6.53 12.33 Comparative Example 5 264.6 249.2 233.5 5.82 11.75 Comparative Example 6 263.9 248.3 231.2 5.91 12.39 Comparative Example 7 265.7 247.6 232.7 6.81 12.42 Comparative Example 8 265.4 247.3 236.1 6.82 11.04 Comparative Example 9 265.7 248.3 235.7 6.55 11.29 Comparative Example 10 264.2 247.9 235.2 6.17 10.98

[0142] According to the test results in Table 2, the mask of the present invention performs well in reducing the content of melanin. After three weeks of continuous use, the reduction in melanin content exceeds 26%. Compared with the embodiment, in Comparative Examples 1-3, only the leaf membrane liquid of Alisma orientalis fermented by Aspergillus niger, Lactobacillus plantarum or Bacillus subtilis, and the conventional leaf alcohol extract of Alisma orientalis used in Comparative Example 4, the content of flavonoids and polyphenols in the fermented species decreases, resulting in a decrease in whitening performance. The mask base fabrics of Comparative Examples 8-10 are not conducive to the loading of the membrane liquid, which is also not conducive to the removal of melanin, resulting in a decrease in whitening performance.

[0143] 4. Skin elasticity test

[0144] In this test, we selected the masks of Examples 1-5 and Comparative Examples 1-10 as samples. The volunteers participating in the test ranged in age from 35 to 50 years old and were randomly assigned to 15 different groups, each containing 10 members. The test required each volunteer to apply a mask three times a week for five weeks. We used the Cutometer skin elasticity tester produced by Courage&Khazaka of Germany to measure skin elasticity. The test was carried out on the 0th, 21st and 35th days. The cheek area was selected as the test point, and the measurement was repeated five times in the same test area to obtain the average value. Among them, the skin elasticity growth rate W% = 100% * (NT-N0) / N0; N0-elasticity test value on the 0th day; NT-elasticity test value during the Tth day. The specific skin elasticity test results are shown in Table 3.

[0145] Table 3 Skin elasticity test

[0146] Test samples Week 0 Week 3 Week 5 Week 3 Growth Rate Week 5 Growth Rate Example 1 0.54 0.61 0.71 12.96 31.48 Example 2 0.58 0.65 0.72 12.07 24.14 Example 3 0.61 0.69 0.79 13.11 29.51 Example 4 0.63 0.70 0.78 11.11 23.81 Example 5 0.64 0.72 0.79 12.50 23.44 Comparative Example 1 0.62 0.64 0.67 3.23 8.06 Comparative Example 2 0.61 0.63 0.65 3.28 6.56 Comparative Example 3 0.62 0.66 0.69 6.45 11.29 Comparative Example 4 0.55 0.59 0.63 7.27 14.55 Comparative Example 5 0.58 0.61 0.66 5.17 13.79 Comparative Example 6 0.56 0.59 0.63 5.36 12.50 Comparative Example 7 0.57 0.61 0.64 7.02 12.28 Comparative Example 8 0.59 0.62 0.69 5.08 16.95 Comparative Example 9 0.58 0.62 0.68 6.90 17.24 Comparative Example 10 0.62 0.66 0.72 6.45 16.13

[0147] According to the test results of Table 3, it can be learned that after using the facial mask of the embodiment of the present invention for five consecutive weeks, the growth rate of skin elasticity exceeds 20%, showing that the facial mask has a significant effect in improving skin elasticity and can give the skin better elasticity and vitality. By comparing Example 1 with Comparative Examples 1-10, it is found that each component in the facial mask of the present invention has a positive promoting effect on enhancing skin elasticity. In particular, in Comparative Examples 1-2, only the leaves of Alisma orientalis fermented with Aspergillus niger or Lactobacillus plantarum cannot be effectively dissolved, and its skin elasticity has a significant decrease. In addition, the facial mask base fabric of Comparative Examples 8-10 lacks one of silk protein fiber, polyvinyl alcohol, and chitosan, resulting in a weakening of the intermolecular force, which is not conducive to the lifting and tightening of the skin, and then leads to a significant decrease in skin elasticity.

[0148] 5. Anti-aging test

[0149] This study recruited volunteers aged 35 to 50 as subjects and evenly distributed them into 15 different groups. The facial mask products prepared by Examples 1-5 and Comparative Examples 1-10 were used respectively. Before the test began, all subjects used the same cleaning product to clean their faces, and then used the American VISIA facial image analyzer to conduct a preliminary test on facial wrinkles. After that, the subjects in each group used the product once every 3 nights. After using the product continuously for 1 month, 2 months and 3 months, the facial wrinkles were tested again using the VISIA facial image analyzer, and the effect of reducing skin wrinkles was analyzed to evaluate the wrinkle removal effect of each test group product and calculate the reduction rate of facial wrinkles. The wrinkle reduction rate results of the anti-aging performance test of each test group product are described in Table 4 below.

[0150] Table 4 Anti-aging performance test

[0151] Test samples Wrinkle reduction rate in 1 month / % 2-month wrinkle reduction rate / % 3-month wrinkle reduction rate / % Example 1 25.33 33.43 45.87 Example 2 26.12 32.81 46.32 Example 3 24.84 32.16 45.98 Example 4 23.68 34.05 45.87 Example 5 26.36 33.57 46.05 Comparative Example 1 15.41 22.31 30.88 Comparative Example 2 14.39 21.94 31.06 Comparative Example 3 13.96 20.88 30.68 Comparative Example 4 14.65 19.97 29.74 Comparative Example 5 15.02 21.36 29.99 Comparative Example 6 14.91 22.07 30.12 Comparative Example 7 13.88 21.48 30.35 Comparative Example 8 14.25 20.69 30.53 Comparative Example 9 14.66 20.41 29.86 Comparative Example 10 13.21 21.06 30.14

[0152] According to the test results in Table 4, the facial mask prepared by the present invention performs well in anti-aging and wrinkle reduction. After three consecutive months of use, the wrinkles on the facial skin were reduced by 45%. The fermented oriental leaves used in Comparative Examples 1-4 and 6-7 contain less flavonoids and polyphenols, and the antioxidant properties are reduced, which is manifested as insufficient in anti-aging and wrinkle reduction. At the same time, Comparative Examples 8-10 lack one of the silk protein fiber, polyvinyl alcohol, and chitosan, which destroys the equilibrium force between molecules, resulting in reduced stability of the mask structure, affecting the effect of lifting and tightening the skin, thereby reducing the anti-wrinkle performance.

[0153] 6. Oil control effect test:

[0154] This study recruited volunteers aged 35 to 50 and evenly distributed them into 15 different groups, each containing 10 members. These groups used the facial mask products prepared by Examples 1-5 and Comparative Examples 1-10, respectively. The Sebumeter SM815 skin oil tester produced by German CK company was used to test the skin oil content before using the mask, recorded as M0, and the oil content of the skin 90 minutes after completing a mask use, recorded as M90, and the skin oil reduction rate was calculated. The calculation formula of the oil reduction rate is: (M90-M0) / M0*100%.

[0155] Table 5 Oil control performance test

[0156] Test samples 0 min oil content Oil content at 90 min Grease reduction rate / % Example 1 210.36 51.38 75.58 Example 2 226.14 62.04 72.57 Example 3 198.64 52.13 73.76 Example 4 220.36 58.96 73.24 Example 5 216.45 55.74 74.25 Comparative Example 1 196.40 103.41 47.35 Comparative Example 2 231.65 145.33 37.26 Comparative Example 3 245.23 163.21 33.45 Comparative Example 4 210.36 120.94 42.51 Comparative Example 5 231.66 153.65 33.67 Comparative Example 6 210.98 125.91 40.32 Comparative Example 7 220.85 140.65 36.31 Comparative Example 8 206.89 129.54 37.39 Comparative Example 9 210.94 136.47 35.30 Comparative Example 10 209.64 139.66 33.38

[0157] According to the experimental data in Table 5, we observed that after using the facial mask of the present invention, the skin oil content decreased by an average of more than 73%, showing a significant oil control effect of the facial mask. Compared with Example 1, the facial masks of Comparative Examples 1-10 lack some key ingredients, although they still have a certain oil control effect, but are obviously not as good as Example 1. In particular, the facial mask base fabrics of Comparative Examples 8-10, due to the lack of a component in silk protein fiber, polyvinyl alcohol or chitosan, destroy the equilibrium force between the base fabric molecules, which is not conducive to the load of the membrane liquid, resulting in a decrease in the oil resistance of the facial mask. Comparative Example 5 omits silk protein, which affects the air permeability and fit of the facial mask, and then affects the penetration and absorption of the effective ingredients, so that the oil resistance is significantly reduced. These experimental results emphasize the importance of the balance of facial mask ingredients to achieve the best oil resistance effect.

[0158] The present invention has been described in detail through the general description, specific embodiments and experimental part. However, it is obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present invention. Therefore, any such modifications or improvements made without departing from the core spirit of the present invention should be regarded as within the scope of the protection claimed by the present invention.

Claims

1. A whitening, moisturizing and anti-wrinkle facial mask, characterized in that: It comprises a membrane liquid and a silk protein fiber-based cloth, wherein the membrane liquid comprises, by weight: 0.5-2 parts of silk protein, 0.3-1.2 parts of vitamin C, 1-3 parts of inositol, 0.1-0.8 parts of oriental leaf fermentation liquid, 0.5-2 parts of ginkgo leaf extract, 0.5-1.5 parts of bird's nest acid, 0.5-2.5 parts of niacinamide, 1-5 parts of auxiliary ingredients and 80-120 parts of water; The silk protein fiber base fabric comprises, by weight: 20-40 parts of silk protein peptide, 5-10 parts of polyvinyl alcohol, and 8-15 parts of chitosan, and the silk protein fiber mask base fabric is prepared by electrospinning; The preparation method of the oriental oriental leaf fermented liquid comprises the following steps: S11: freeze-dry and grind fresh oriental oriental leaves into oriental ... S12: sterilize and cool the mixed solution prepared in S11, inoculate probiotics, and then ferment and culture. After centrifugation, take the supernatant to obtain the fermented liquid of Alisma orientalis leaves; the inoculation amount of the probiotics relative to the mixed solution is 0.5-15 vol%; the probiotics are Aspergillus niger and Lactobacillus plantarum mixed in a volume ratio of 1:3-6, and the effective live bacteria count of Aspergillus niger and Lactobacillus plantarum is 1×10 8 -8×10 8 CFU / mL; The ginkgo leaf extract is prepared as follows: Ginkgo leaves with intact leaves and normal color are selected, and the ginkgo leaves are freeze-dried and crushed to obtain ginkgo leaf powder for later use. The ginkgo leaf powder is weighed and added to 50% ethanol solution at a mass ratio of 1:30-50, stirred and soaked, and extracted with ultrasonic vibration for 1-3h, extracted twice, centrifuged, the supernatant was collected and combined, and the solvent was removed to obtain ginkgo leaf extract.

2. The whitening, moisturizing and anti-wrinkle facial mask according to claim 1, characterized in that: The fermentation culture conditions are as follows: fermentation temperature is 30-45° C., initial pH of the mixed solution is 5-7, and fermentation time is 12-48 hours.

3. The whitening, moisturizing and anti-wrinkle facial mask according to claim 1, characterized in that: The inoculation amount of the probiotics relative to the mixed solution is 5-15 vol%.

4. The whitening, moisturizing and anti-wrinkle facial mask according to claim 1, characterized in that: The auxiliary components include, by weight, 0.10-1.00 parts of a thickener, 0.02-0.10 parts of a chelating agent, 0.2-10 parts of a polyol, and 0.5-2 parts of a pH regulator.

5. The whitening, moisturizing and anti-wrinkle facial mask according to claim 4, characterized in that: The thickener is at least one of sodium polyacrylate, carboxymethyl cellulose, hydroxyethyl cellulose and xanthan gum; the chelating agent is at least one of disodium ethylenediaminetetraacetate, trisodium ethylenediaminetetraacetate, tetrasodium ethylenediaminetetraacetate, ethylenediaminetetraacetic acid and trisodium ethylenediamine disuccinate; the polyol is one or more of glycerol, butylene glycol and propylene glycol; the pH adjuster is at least one of aminomethyl propanol, arginine, citric acid and sodium citrate.

6. A method for preparing the whitening, moisturizing and anti-wrinkle facial mask according to any one of claims 1 to 5, characterized in that: The following steps are included: 1) Preparation of mask solution: weigh 0.5-2 parts of silk protein, 0.3-1.2 parts of vitamin C, 1-3 parts of inositol, 0.1-0.8 parts of oriental leaf fermentation liquid, 0.5-2 parts of ginkgo leaf extract, 0.5-1.5 parts of bird's nest acid, 0.5-2.5 parts of niacinamide, 1-5 parts of auxiliary ingredients and 80-120 parts of water, stir and mix evenly at 40-60° C. to obtain mask solution; 2) Silk protein fiber base fabric, using electrospinning to prepare the silk protein fiber mask base fabric; 3) Preparation of a whitening, moisturizing and anti-wrinkle emulsion mask: Soak the base fabric in a membrane solution 40 to 60 times its mass for 30 to 60 minutes at 15 to 30°C to prepare a whitening, moisturizing and anti-wrinkle emulsion mask.

7. The method for preparing the whitening, moisturizing and anti-wrinkle facial mask according to claim 6, characterized in that: The preparation of the silk protein fiber-based fabric comprises the following steps: S21: weighing 20-40 parts of silk protein peptide, 5-10 parts of polyvinyl alcohol, and 8-15 parts of chitosan; dissolving the polyvinyl alcohol in deionized water to prepare a polyvinyl alcohol solution with a mass fraction of 10%-15%; then adding the silk protein peptide and chitosan to the prepared polyvinyl alcohol solution to obtain a mixed solution; S22: stirring the mixed solution obtained in step S21 at a constant temperature until a uniform solution is formed, and then standing to degas for standby use to obtain a spinning solution; S23: electrospinning the spinning solution, and obtaining a mask base fabric containing silk protein fibers after drying the product.

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